Chy7G143760 (gene) Cucumber (hystrix) v1

Overview


Name	Chy7G143760
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	sterol 14-demethylase
Location	chrH07: 19702299 .. 19704064 (+)
RNA-Seq Expression	Chy7G143760
Synteny	Chy7G143760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACCTGATAACATCAAATTCTTCAATGTGGGTCTTCTTCTTCTCGCCACTTTACTCCTCGCAAAGCTTATTTCTGCTCTTCTCAATTCCAGATCTAACAAACGGCCTCCTCCCACTGTTAAAGGCATCCCCTTTATTGGGGGATTGCTCCGTTTTCTTAAAGGTCCGATTGTGATGCTGAGGAAGGAGTACCCTAAACTTGGGAGTGTTTTCACCGTTAACTTATTGCATAAAAAAATTACCTTCTTGATTGGCCCTGAGGTTTCTGCACATTTCTTCAAAGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTCCCAACTTTTGGCCCTGGCGTTGTGTTCGATGTGGATTACTCCATTCGTCAAGAACAGTTCAGGTTCTTCACTGAGGCTCTCAGAGTCACAAAACTGAAAGGATACGTAGATCAGATGGTTAGAGAAGCAGAGGTCAGTATCAATGCATCATAGATCTAATAAATTGATAAGAATTTAAGAATGAAAGTGAAAGGACTATAATATTCTTATTCATTTAATGCAAGCAAATGTTAAGGATTGTAAATGTTAGGATTCTCTAAGAACCTGTGCTCTGTCTAGTTTTACTCATCACCTTGCTAATGTGAATGCATGCTAAATTTAACATCCATCCAACTTTTCTGATGATTACGTCCAAATCTTCGATTTCCATTCTCTTTGTTGTACATTATTCTTTAGAATCTGTGTAACCTTGGGATGTGGTGGGGTATTTTGAGCAGGATTTCTTCTCTAAGTGGGGTGACTGTGGGGAGGTGGATCTCAAGTATGAGTTGGAACATCTTATCATCTTAACAGCTAGTAGATGCCTTTTGGGAAGTGAGGTTCGTGATAAGCTTTTTGCGGATGTTTCAGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTTATGTTTCCGTACCTTCCTATCCCGGCTCATCGTCGTCGTGATCAAGCACGCAGCAAGCTTGCGGATATCTTTGCAAAAATCATAGCTTCCAGAAAATGCAATGGGAGTTCAGATAATGATATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGGCGGTCAACAACAGATTCTGAAGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACATACCAGTTCTATTACCTCAACTTGGACTGGGGCTTATCTCCTATGCCACAAAGAGTACATGTCTGCTGTTTTAGAGGAACAGCAAAGGCTGATGGGTAAGCATGGCGACAAGATTGATCATGATATCTTGTCTGAAATGGACAATCTCCATAGGTGCATTAAAGAAGCTCTCAGACTTCACCCTCCATTGATTATGTTGATGCGAAGTTCGCATAGTGATTTTAGTGTTACAACCAGAGAGGGGAAAGAATATGATATTCCAAAGGGTCATATAATTGCCACATCTCCAGCTTTTGCTAATCGACTTCCTCATGTGTATAAGGATCCAGACAGGTATGATCCAGATAGGTTCGCCCCTGGGAGAGATGAAGACAAGGCGGCTGGACCGTTCTCCTACATCTCGTTCGGAGGTGGCAGGCATGGGTGCCTTGGCGAACCCTTTGCTTATCTACAGATAAAAGCAATATGGAGTCAGTTGCTAAGAAATTTCGAACTGGAGCTAATATCTCCGTTTCCTGAAATTGATTGGAATGCTATGGTTGTGGGTGTCAAAGGCAAAGTTATGGTCAGGTACAAGAGAAGGAAGCTTTCCTGTTAG

mRNA sequence

ATGGAACCTGATAACATCAAATTCTTCAATGTGGGTCTTCTTCTTCTCGCCACTTTACTCCTCGCAAAGCTTATTTCTGCTCTTCTCAATTCCAGATCTAACAAACGGCCTCCTCCCACTGTTAAAGGCATCCCCTTTATTGGGGGATTGCTCCGTTTTCTTAAAGGTCCGATTGTGATGCTGAGGAAGGAGTACCCTAAACTTGGGAGTGTTTTCACCGTTAACTTATTGCATAAAAAAATTACCTTCTTGATTGGCCCTGAGGTTTCTGCACATTTCTTCAAAGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTCCCAACTTTTGGCCCTGGCGTTGTGTTCGATGTGGATTACTCCATTCGTCAAGAACAGTTCAGGTTCTTCACTGAGGCTCTCAGAGTCACAAAACTGAAAGGATACGTAGATCAGATGGTTAGAGAAGCAGAGGATTTCTTCTCTAAGTGGGGTGACTGTGGGGAGGTGGATCTCAAGTATGAGTTGGAACATCTTATCATCTTAACAGCTAGTAGATGCCTTTTGGGAAGTGAGGTTCGTGATAAGCTTTTTGCGGATGTTTCAGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTTATGTTTCCGTACCTTCCTATCCCGGCTCATCGTCGTCGTGATCAAGCACGCAGCAAGCTTGCGGATATCTTTGCAAAAATCATAGCTTCCAGAAAATGCAATGGGAGTTCAGATAATGATATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGGCGGTCAACAACAGATTCTGAAGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACATACCAGTTCTATTACCTCAACTTGGACTGGGGCTTATCTCCTATGCCACAAAGAGTACATGTCTGCTGTTTTAGAGGAACAGCAAAGGCTGATGGGTAAGCATGGCGACAAGATTGATCATGATATCTTGTCTGAAATGGACAATCTCCATAGGTGCATTAAAGAAGCTCTCAGACTTCACCCTCCATTGATTATGTTGATGCGAAGTTCGCATAGTGATTTTAGTGTTACAACCAGAGAGGGGAAAGAATATGATATTCCAAAGGGTCATATAATTGCCACATCTCCAGCTTTTGCTAATCGACTTCCTCATGTGTATAAGGATCCAGACAGGTATGATCCAGATAGGTTCGCCCCTGGGAGAGATGAAGACAAGGCGGCTGGACCGTTCTCCTACATCTCGTTCGGAGGTGGCAGGCATGGGTGCCTTGGCGAACCCTTTGCTTATCTACAGATAAAAGCAATATGGAGTCAGTTGCTAAGAAATTTCGAACTGGAGCTAATATCTCCGTTTCCTGAAATTGATTGGAATGCTATGGTTGTGGGTGTCAAAGGCAAAGTTATGGTCAGGTACAAGAGAAGGAAGCTTTCCTGTTAG

Coding sequence (CDS)

ATGGAACCTGATAACATCAAATTCTTCAATGTGGGTCTTCTTCTTCTCGCCACTTTACTCCTCGCAAAGCTTATTTCTGCTCTTCTCAATTCCAGATCTAACAAACGGCCTCCTCCCACTGTTAAAGGCATCCCCTTTATTGGGGGATTGCTCCGTTTTCTTAAAGGTCCGATTGTGATGCTGAGGAAGGAGTACCCTAAACTTGGGAGTGTTTTCACCGTTAACTTATTGCATAAAAAAATTACCTTCTTGATTGGCCCTGAGGTTTCTGCACATTTCTTCAAAGCATCTGAGTCTGATCTTAGCCAGCAGGAAGTTTACCAGTTTAATGTCCCAACTTTTGGCCCTGGCGTTGTGTTCGATGTGGATTACTCCATTCGTCAAGAACAGTTCAGGTTCTTCACTGAGGCTCTCAGAGTCACAAAACTGAAAGGATACGTAGATCAGATGGTTAGAGAAGCAGAGGATTTCTTCTCTAAGTGGGGTGACTGTGGGGAGGTGGATCTCAAGTATGAGTTGGAACATCTTATCATCTTAACAGCTAGTAGATGCCTTTTGGGAAGTGAGGTTCGTGATAAGCTTTTTGCGGATGTTTCAGCTTTGTTTCATGACCTTGACAATGGAATGCTCCCAATCAGTGTTATGTTTCCGTACCTTCCTATCCCGGCTCATCGTCGTCGTGATCAAGCACGCAGCAAGCTTGCGGATATCTTTGCAAAAATCATAGCTTCCAGAAAATGCAATGGGAGTTCAGATAATGATATGTTGCAATGTTTTATTGACTCAAAGTACAAAGATGGGCGGTCAACAACAGATTCTGAAGTCACTGGTTTGCTCATAGCTGCTCTTTTTGCAGGTCAACATACCAGTTCTATTACCTCAACTTGGACTGGGGCTTATCTCCTATGCCACAAAGAGTACATGTCTGCTGTTTTAGAGGAACAGCAAAGGCTGATGGGTAAGCATGGCGACAAGATTGATCATGATATCTTGTCTGAAATGGACAATCTCCATAGGTGCATTAAAGAAGCTCTCAGACTTCACCCTCCATTGATTATGTTGATGCGAAGTTCGCATAGTGATTTTAGTGTTACAACCAGAGAGGGGAAAGAATATGATATTCCAAAGGGTCATATAATTGCCACATCTCCAGCTTTTGCTAATCGACTTCCTCATGTGTATAAGGATCCAGACAGGTATGATCCAGATAGGTTCGCCCCTGGGAGAGATGAAGACAAGGCGGCTGGACCGTTCTCCTACATCTCGTTCGGAGGTGGCAGGCATGGGTGCCTTGGCGAACCCTTTGCTTATCTACAGATAAAAGCAATATGGAGTCAGTTGCTAAGAAATTTCGAACTGGAGCTAATATCTCCGTTTCCTGAAATTGATTGGAATGCTATGGTTGTGGGTGTCAAAGGCAAAGTTATGGTCAGGTACAAGAGAAGGAAGCTTTCCTGTTAG

Protein sequence

MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILTASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKLSC*

Homology

[Close]

BLAST of Chy7G143760 vs. ExPASy Swiss-Prot
Match: Q9SAA9 (Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 7.4e-235
Identity = 395/481 (82.12%), Postives = 439/481 (91.27%), Query Frame = 0


Query: 7   KFFNVGLLLLATLLLAKLISALLNSRS-NKRPPPTVKG-IPFIGGLLRFLKGPIVMLRKE 66
           K    GL+++ATL++AKLI +   S S  KR PPT+K   P +G L++FLKGPI+MLR+E
Sbjct: 8   KLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPIIMLREE 67

Query: 67  YPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 126
           YPKLGSVFTVNL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 68  YPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 127

Query: 127 SIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILTASRC 186
           S+RQEQFRFFTEALRV KLKGYVD MV EAED+FSKWG+ GEVD+K ELE LIILTASRC
Sbjct: 128 SVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIILTASRC 187

Query: 187 LLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIAS 246
           LLG EVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD+AR KL++IFAKII S
Sbjct: 188 LLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFAKIIGS 247

Query: 247 RKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCH 306
           RK +G ++NDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWTGAYL+ +
Sbjct: 248 RKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGAYLMRY 307

Query: 307 KEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFSV 366
           KEY SA L+EQ+ L+ KHGDKIDHDILSEMD L+RCIKEALRLHPPLIMLMR+SHSDFSV
Sbjct: 308 KEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASHSDFSV 367

Query: 367 TTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFG 426
           T R+GK YDIPKGHI+ATSPAFANRLPH++KDPD YDP+RF+PGR+EDKAAG FSYI+FG
Sbjct: 368 TARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFG 427

Query: 427 GGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKL 486
           GGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRYKRR+L
Sbjct: 428 GGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRYKRRQL 487

[Close]

BLAST of Chy7G143760 vs. ExPASy Swiss-Prot
Match: P93596 (Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP51 PE=2 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 6.7e-220
Identity = 360/449 (80.18%), Postives = 408/449 (90.87%), Query Frame = 0


Query: 36  RPPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFK 95
           RPPPT+ G P +GGLLRFL+GPI ++R EY +LG VFTV +L ++ITFLIGP+VSAHFFK
Sbjct: 1   RPPPTIPGAPVVGGLLRFLRGPIPLIRAEYARLGPVFTVPILTRRITFLIGPDVSAHFFK 60

Query: 96  ASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAE 155
           ++ESD+SQQEVY+FNVPTFGPGVVFDVDY +RQEQFRFFTEALR  KL+ YVDQMV EAE
Sbjct: 61  SNESDMSQQEVYRFNVPTFGPGVVFDVDYQVRQEQFRFFTEALRANKLRSYVDQMVAEAE 120

Query: 156 DFFSKWGDCGEVDLKYELEHLIILTASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVM 215
           ++FSKWG+ G VDLKYELEHLIILTASRCLLG EVR+KLF DVSALFHDLDNGMLPISV+
Sbjct: 121 EYFSKWGESGTVDLKYELEHLIILTASRCLLGREVREKLFDDVSALFHDLDNGMLPISVI 180

Query: 216 FPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEV 275
           FPYLPIPAHRRRDQAR++LA+IFA II SRK +G S+ DMLQCFIDSKYK+GR TT+SEV
Sbjct: 181 FPYLPIPAHRRRDQARTRLAEIFATIIKSRKASGQSEEDMLQCFIDSKYKNGRQTTESEV 240

Query: 276 TGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMD 335
           TGLLIAALFAGQHTSSITSTWTGAYLL  ++Y +  +EEQ+ +M +HGDKIDHDIL+EMD
Sbjct: 241 TGLLIAALFAGQHTSSITSTWTGAYLLKFQQYFAEAVEEQKEVMKRHGDKIDHDILAEMD 300

Query: 336 NLHRCIKEALRLHPPLIMLMRSSHSDFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYK 395
            L+RCIKEALRLHPPLIML+R SHSDFSVTTREGKE+DIPKGHI+ATSPAFANRLPH++K
Sbjct: 301 VLYRCIKEALRLHPPLIMLLRQSHSDFSVTTREGKEFDIPKGHIVATSPAFANRLPHIFK 360

Query: 396 DPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELEL 455
           +PD YDPDRFA GR+EDK AG FSYISFGGGRHGCLGEPFAYLQIKAIW+ LLRNFE EL
Sbjct: 361 NPDSYDPDRFAAGREEDKVAGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEFEL 420

Query: 456 ISPFPEIDWNAMVVGVKGKVMVRYKRRKL 485
           +SPFPE DWNAMVVG+KG+VMV YKRRKL
Sbjct: 421 VSPFPENDWNAMVVGIKGEVMVNYKRRKL 449

[Close]

BLAST of Chy7G143760 vs. ExPASy Swiss-Prot
Match: P93846 (Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 4.4e-219
Identity = 357/471 (75.80%), Postives = 420/471 (89.17%), Query Frame = 0


Query: 14  LLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKLGSVFT 73
           L++AT++  KL+ +  +    KR PPT+ G P +GGL++F++GPI M+R++Y  LGSVFT
Sbjct: 18  LVVATVIFLKLLLSFRSGGGKKRLPPTIPGAPVVGGLVKFMRGPIPMIREQYAALGSVFT 77

Query: 74  VNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRF 133
           V ++ ++ITFLIGPEVSAHFFK +E+++SQQEVY+FNVPTFGPGVVFDVDYS+RQEQFRF
Sbjct: 78  VPIITRRITFLIGPEVSAHFFKGNEAEMSQQEVYRFNVPTFGPGVVFDVDYSVRQEQFRF 137

Query: 134 FTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILTASRCLLGSEVRDK 193
           FTEALR  KL+ YVDQMV EAE++FSKWG+ G VDLKYELEHLIILTASRCLLG EVR+K
Sbjct: 138 FTEALRANKLRSYVDQMVAEAEEYFSKWGESGTVDLKYELEHLIILTASRCLLGREVREK 197

Query: 194 LFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSDN 253
           LF DVSALFHDLDNG+ PISV+FPYLPIPAH+RRD+AR++LA+IFA II SRK +G S+ 
Sbjct: 198 LFDDVSALFHDLDNGIQPISVLFPYLPIPAHKRRDKARARLAEIFATIIKSRKASGQSEE 257

Query: 254 DMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVLE 313
           DMLQCFIDSKYK+GR TT+ EVTGLLIAALFAGQHTSSITSTWTGAY+L  K+Y +  +E
Sbjct: 258 DMLQCFIDSKYKNGRPTTEGEVTGLLIAALFAGQHTSSITSTWTGAYMLRFKQYFAEAVE 317

Query: 314 EQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFSVTTREGKEYD 373
           EQ+ +M +HGDKIDHDIL+EMD L+RCIKEALRLHPPLIML+R SHSDF+VTT+EGKEYD
Sbjct: 318 EQKDVMKRHGDKIDHDILAEMDVLYRCIKEALRLHPPLIMLLRQSHSDFTVTTKEGKEYD 377

Query: 374 IPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHGCLGE 433
           IPKGHI+ATSP+FANRLPH+YK+PD YDPDRF PGR+EDKAAG FSYISFGGGRHGCLGE
Sbjct: 378 IPKGHIVATSPSFANRLPHIYKNPDSYDPDRFGPGREEDKAAGAFSYISFGGGRHGCLGE 437

Query: 434 PFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKL 485
           PFAYLQIKAIW+ LLRNFE EL+SPFPE DWNAMVVG+KG+VMV YKRRKL
Sbjct: 438 PFAYLQIKAIWTHLLRNFEFELVSPFPENDWNAMVVGIKGEVMVNYKRRKL 488

[Close]

BLAST of Chy7G143760 vs. ExPASy Swiss-Prot
Match: Q1ZXH9 (Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN=cyp51 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.6e-104
Identity = 212/474 (44.73%), Postives = 294/474 (62.03%), Query Frame = 0


Query: 16  LATLLLAKL-ISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKLGSVFTV 75
           +A L++A L I A   S SN   PP V+ IPFIG   +F K P+ ++R  Y +LG +FT+
Sbjct: 5   IAVLVIAILVIFAFKKSPSN--IPPIVETIPFIGCFYQFAKNPLQLVRNSYDRLGEIFTL 64

Query: 76  NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFF 135
           +L+  K+TF++GPE  A FF+ ++ +LS +E Y+F  P FG GVV+D +  I  EQ RF 
Sbjct: 65  HLMGFKMTFVLGPEAQALFFRGTDEELSPKEAYRFVTPVFGKGVVYDSETEIMYEQLRFV 124

Query: 136 TEALRVTKLKGYVDQMVREAEDFF-SKWGDCGEVDLKYELEHLIILTASRCLLGSEVRDK 195
              L +++LK  V  +  E E +F +KWGD GE+DL YE+  L ILTASRCL+G  +   
Sbjct: 125 KNGLVLSQLKKAVGIIQEETEKYFETKWGDSGEIDLLYEMNKLTILTASRCLMGKSINKS 184

Query: 196 L--FADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIAS-RKCNGS 255
           L     ++ L+H+L+ G+ PIS  FP LP+P+ ++RD AR+K+A IF  II   R+    
Sbjct: 185 LGQSGQLADLYHELEEGLNPISFFFPNLPLPSFKKRDAARAKVAAIFHSIIQERRRSTDD 244

Query: 256 SDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSA 315
           S +D+L   ++SKYKDG    D ++ GL+I  LFAGQHTSSIT T+T  YLL + EY   
Sbjct: 245 SVDDVLYTLMNSKYKDGSVLEDEQIVGLMIGLLFAGQHTSSITLTYTIFYLLNNLEYFDE 304

Query: 316 VLEEQQRLMGKHGD-KIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFSVTTREG 375
             ++   ++ K    +I+ D L  M+ L   I+E LRLHPPLI LMR       +T  E 
Sbjct: 305 TQKDINDIVQKENQGEINFDGLKRMNRLETVIREVLRLHPPLIFLMRK-----VMTPMEY 364

Query: 376 KEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRHG 435
           K   IP GHI+A SP    RLP VYK+PD ++P RF     EDK   PFS+I+FGGG+HG
Sbjct: 365 KGKTIPAGHILAVSPQVGMRLPTVYKNPDSFEPKRFDV---EDKT--PFSFIAFGGGKHG 424

Query: 436 CLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRK 484
           C GE F  LQIK IW+ L   + LE + P P  D+ ++V G KG  MV+Y +++
Sbjct: 425 CPGENFGILQIKTIWTVLSTKYNLE-VGPVPPTDFTSLVAGPKGPCMVKYSKKQ 465

[Close]

BLAST of Chy7G143760 vs. ExPASy Swiss-Prot
Match: Q4PJW3 (Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 8.5e-90
Identity = 184/477 (38.57%), Postives = 269/477 (56.39%), Query Frame = 0


Query: 9   FNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVMLRKEYPKL 68
           F + L+ L  L +  L   L      K PP  V  IPF+G  + F K PI  L   Y K 
Sbjct: 34  FTLSLVYLFRLAVGHLAPPL--PTGAKSPPYIVSPIPFLGHAIAFGKSPIEFLEDAYEKY 93

Query: 69  GSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVY-QFNVPTFGPGVVFDVDYSIR 128
           G VF+  ++ K  T+L+G E +A  F +   DL+ +EVY +   P FG GV +DV  ++ 
Sbjct: 94  GPVFSFTMVGKTFTYLLGSEAAALLFNSKNEDLNAEEVYSRLTTPVFGKGVAYDVPNTVF 153

Query: 129 QEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILTASRCLLG 188
            EQ +     L +   + +V  + +E +++F  WG+ GE +L   L  LIILTAS CL G
Sbjct: 154 LEQKKMLKSGLNIAHFRQHVSIIEKETKEYFKSWGESGEKNLFEALSELIILTASHCLHG 213

Query: 189 SEVRDKLFADVSALFHDLDNGMLPISVMFP-YLPIPAHRRRDQARSKLADIFAKIIASRK 248
            E+R +L   V+ L+ DLD G    + + P +LP+P+ RRRD+A  ++ +IF K I  R+
Sbjct: 214 KEIRSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKNIFYKAIQKRR 273

Query: 249 CNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKE 308
            +G   +D+LQ  ++S YKDGR  TD EV G+LI  L AGQHTSS TS W G +L   K 
Sbjct: 274 ESGEKIDDILQTLLESTYKDGRPLTDDEVAGMLIGLLLAGQHTSSTTSAWMGFFLARDKT 333

Query: 309 YMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFSVTT 368
                  EQ+ + G++   + +D L +++ L RCIKE LRL PP++ +MR + +  +V  
Sbjct: 334 LQEKCFLEQKTVCGENLPPLTYDQLKDLNLLDRCIKETLRLRPPIMTMMRLAKTPLTVA- 393

Query: 369 REGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGG 428
                Y IP GH +  SP    RL   + +   ++PDR+    D   +   F+Y+ FG G
Sbjct: 394 ----GYTIPPGHQVCVSPTVNQRLKDSWVERLDFNPDRYL--EDSPASGEKFAYVPFGAG 453

Query: 429 RHGCLGEPFAYLQIKAIWSQLLRNFELELISP-FPEIDWNAMVVGVKGKVMVRYKRR 483
           RH C+GE FAY+QIK IWS +LR +E +LI   FP +++  M +    K ++RYKRR
Sbjct: 454 RHRCIGENFAYVQIKTIWSTMLRLYEFDLIDGYFPTVNYTTM-IHTPEKPIIRYKRR 500

[Close]

BLAST of Chy7G143760 vs. ExPASy TrEMBL
Match: A0A0A0KYP3 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1)

HSP 1 Score: 975.3 bits (2520), Expect = 9.3e-281
Identity = 481/485 (99.18%), Postives = 482/485 (99.38%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDNIKFFNVGLL+LATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNIKFFNVGLLVLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQ LMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQELMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAG FSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGAFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 486
           RRKLS
Sbjct: 481 RRKLS 485

[Close]

BLAST of Chy7G143760 vs. ExPASy TrEMBL
Match: A0A5A7TLX5 (Sterol 14-demethylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G002430 PE=3 SV=1)

HSP 1 Score: 973.4 bits (2515), Expect = 3.5e-280
Identity = 481/485 (99.18%), Postives = 482/485 (99.38%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KFFNVGLLLLATLLLAKLISALLNSRSNKR PPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 486
           RRKLS
Sbjct: 481 RRKLS 485

[Close]

BLAST of Chy7G143760 vs. ExPASy TrEMBL
Match: A0A1S3BXE7 (sterol 14-demethylase OS=Cucumis melo OX=3656 GN=LOC103494635 PE=3 SV=1)

HSP 1 Score: 973.4 bits (2515), Expect = 3.5e-280
Identity = 481/485 (99.18%), Postives = 482/485 (99.38%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KFFNVGLLLLATLLLAKLISALLNSRSNKR PPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 486
           RRKLS
Sbjct: 481 RRKLS 485

[Close]

BLAST of Chy7G143760 vs. ExPASy TrEMBL
Match: A0A6J1J0F8 (sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1)

HSP 1 Score: 936.4 bits (2419), Expect = 4.8e-269
Identity = 462/485 (95.26%), Postives = 475/485 (97.94%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KF NVGLL++ATLLLAK+ISA+LNSRSNKR PPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 486
           RRKLS
Sbjct: 481 RRKLS 484

[Close]

BLAST of Chy7G143760 vs. ExPASy TrEMBL
Match: A0A6J1GUN6 (sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.3e-268
Identity = 460/485 (94.85%), Postives = 474/485 (97.73%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KF NVGLL++ATLLLAK+ISA+LNSRSNKR PPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYP+LG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPELGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRV KLKGYVDQMV EAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVAKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 486
           RRKLS
Sbjct: 481 RRKLS 484

[Close]

BLAST of Chy7G143760 vs. NCBI nr
Match: NP_001295809.1 (sterol 14-demethylase [Cucumis sativus] >AIY67848.1 cytochrome P450 [Cucumis sativus] >KGN52966.1 hypothetical protein Csa_014475 [Cucumis sativus])

HSP 1 Score: 973 bits (2516), Expect = 0.0
Identity = 481/485 (99.18%), Postives = 482/485 (99.38%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDNIKFFNVGLL+LATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNIKFFNVGLLVLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQ LMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQELMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAG FSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGAFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 485
           RRKLS
Sbjct: 481 RRKLS 485

[Close]

BLAST of Chy7G143760 vs. NCBI nr
Match: XP_008454145.1 (PREDICTED: sterol 14-demethylase [Cucumis melo] >KAA0044503.1 sterol 14-demethylase [Cucumis melo var. makuwa] >TYK29631.1 sterol 14-demethylase [Cucumis melo var. makuwa])

HSP 1 Score: 971 bits (2511), Expect = 0.0
Identity = 481/485 (99.18%), Postives = 482/485 (99.38%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KFFNVGLLLLATLLLAKLISALLNSRSNKR PPTVKGIPFIGGLLRFLKGPIVM
Sbjct: 1   MEPDNNKFFNVGLLLLATLLLAKLISALLNSRSNKRAPPTVKGIPFIGGLLRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 485
           RRKLS
Sbjct: 481 RRKLS 485

[Close]

BLAST of Chy7G143760 vs. NCBI nr
Match: XP_038882542.1 (sterol 14-demethylase [Benincasa hispida])

HSP 1 Score: 946 bits (2445), Expect = 0.0
Identity = 467/485 (96.29%), Postives = 477/485 (98.35%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           M+PDN KFFNVGLL+LATL LAKLIS +LNSRSNKRPPPTVKG PFIGGLLRFLKGPI+M
Sbjct: 1   MDPDN-KFFNVGLLILATLFLAKLISVVLNSRSNKRPPPTVKGFPFIGGLLRFLKGPIMM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLGSVFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGSVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRKCNGSS+NDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKCNGSSENDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYM+AVLEEQQRLMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSHS
Sbjct: 301 LLRHKEYMAAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHS 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DF+VTTREGKEYDIPKGHIIATSPAFANRL HVYKDP+RYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFTVTTREGKEYDIPKGHIIATSPAFANRLSHVYKDPNRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWS LLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 485
           RRKLS
Sbjct: 481 RRKLS 484

[Close]

BLAST of Chy7G143760 vs. NCBI nr
Match: XP_023527569.1 (sterol 14-demethylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 462/485 (95.26%), Postives = 475/485 (97.94%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KF NVGLL++ATLLLAK+ISA+LNSRSNKR PPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVTEAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 485
           RRKLS
Sbjct: 481 RRKLS 484

[Close]

BLAST of Chy7G143760 vs. NCBI nr
Match: XP_022980879.1 (sterol 14-demethylase-like [Cucurbita maxima])

HSP 1 Score: 935 bits (2416), Expect = 0.0
Identity = 462/485 (95.26%), Postives = 475/485 (97.94%), Query Frame = 0


Query: 1   MEPDNIKFFNVGLLLLATLLLAKLISALLNSRSNKRPPPTVKGIPFIGGLLRFLKGPIVM 60
           MEPDN KF NVGLL++ATLLLAK+ISA+LNSRSNKR PPTVKG P +GGL+RFLKGPIVM
Sbjct: 1   MEPDN-KFLNVGLLVVATLLLAKVISAVLNSRSNKRAPPTVKGFPLVGGLVRFLKGPIVM 60

Query: 61  LRKEYPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120
           LRKEYPKLG+VFT+NLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 61  LRKEYPKLGAVFTLNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVF 120

Query: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILT 180
           DVDYSIRQEQFRFFTEALRVTKLKGYVDQMV EAEDFFSKWGD GEVDLKYELEHLIILT
Sbjct: 121 DVDYSIRQEQFRFFTEALRVTKLKGYVDQMVSEAEDFFSKWGDSGEVDLKYELEHLIILT 180

Query: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAK 240
           ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRD+ARSKLADIFAK
Sbjct: 181 ASRCLLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDRARSKLADIFAK 240

Query: 241 IIASRKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300
           IIASRK +GSS+NDMLQCFIDSKYKDGR TTDSEVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 241 IIASRKSSGSSENDMLQCFIDSKYKDGRPTTDSEVTGLLIAALFAGQHTSSITSTWTGAY 300

Query: 301 LLCHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHS 360
           LL HKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNL+RCIKEALRLHPPLIMLMRSSH+
Sbjct: 301 LLRHKEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLYRCIKEALRLHPPLIMLMRSSHT 360

Query: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420
           DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY
Sbjct: 361 DFSVTTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSY 420

Query: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480
           ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK
Sbjct: 421 ISFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYK 480

Query: 481 RRKLS 485
           RRKLS
Sbjct: 481 RRKLS 484

[Close]

BLAST of Chy7G143760 vs. TAIR 10
Match: AT1G11680.1 (CYTOCHROME P450 51G1 )

HSP 1 Score: 814.3 bits (2102), Expect = 5.3e-236
Identity = 395/481 (82.12%), Postives = 439/481 (91.27%), Query Frame = 0


Query: 7   KFFNVGLLLLATLLLAKLISALLNSRS-NKRPPPTVKG-IPFIGGLLRFLKGPIVMLRKE 66
           K    GL+++ATL++AKLI +   S S  KR PPT+K   P +G L++FLKGPI+MLR+E
Sbjct: 8   KLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPIIMLREE 67

Query: 67  YPKLGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 126
           YPKLGSVFTVNL+HKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 68  YPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDY 127

Query: 127 SIRQEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILTASRC 186
           S+RQEQFRFFTEALRV KLKGYVD MV EAED+FSKWG+ GEVD+K ELE LIILTASRC
Sbjct: 128 SVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLIILTASRC 187

Query: 187 LLGSEVRDKLFADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIAS 246
           LLG EVRD+LF DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD+AR KL++IFAKII S
Sbjct: 188 LLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEIFAKIIGS 247

Query: 247 RKCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCH 306
           RK +G ++NDMLQCFI+SKYKDGR TT+SEVTGLLIAALFAGQHTSSITSTWTGAYL+ +
Sbjct: 248 RKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWTGAYLMRY 307

Query: 307 KEYMSAVLEEQQRLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFSV 366
           KEY SA L+EQ+ L+ KHGDKIDHDILSEMD L+RCIKEALRLHPPLIMLMR+SHSDFSV
Sbjct: 308 KEYFSAALDEQKNLIAKHGDKIDHDILSEMDVLYRCIKEALRLHPPLIMLMRASHSDFSV 367

Query: 367 TTREGKEYDIPKGHIIATSPAFANRLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFG 426
           T R+GK YDIPKGHI+ATSPAFANRLPH++KDPD YDP+RF+PGR+EDKAAG FSYI+FG
Sbjct: 368 TARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFG 427

Query: 427 GGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKRRKL 486
           GGRHGCLGEPFAYLQIKAIWS LLRNFELEL+SPFPEIDWNAMVVGVKG VMVRYKRR+L
Sbjct: 428 GGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRYKRRQL 487

[Close]

BLAST of Chy7G143760 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 129.8 bits (325), Expect = 6.0e-30
Identity = 116/465 (24.95%), Postives = 200/465 (43.01%), Query Frame = 0


Query: 19  LLLAKLISALLNSRSNKRP-PPTVKGIPFIGGLLR-FLKGPIVMLRKEYPKLGSVFTVNL 78
           L L + I+ +  S S K P PP   G P++G   + + + P V    +  + GSVF  ++
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHV 76

Query: 79  LHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTE 138
           L      +  PE +A F   ++S L +           G   +F        +  +    
Sbjct: 77  LGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLR 136

Query: 139 ALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILTASRCLLGSEVRDKLF- 198
           A     ++  V  +   A++  + W D  +++   E++      A   +LG   +D+++ 
Sbjct: 137 AFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISILG---KDEVYY 196

Query: 199 -ADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSD 258
             D+   ++ L+ G   +PI+     LP     +  +AR +LA I A I++ R+ N SS 
Sbjct: 197 REDLKRCYYILEKGYNSMPIN-----LPGTLFHKAMKARKELAQILANILSKRRQNPSSH 256

Query: 259 NDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVL 318
            D+L  F++ K       TD ++   +I  +FA + T++   TW   YL  +   + AV 
Sbjct: 257 TDLLGSFMEDK----AGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVT 316

Query: 319 EEQQ--RLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFSVTTREGK 378
           EEQ   R   K G+ +  +   +M   +R I+E LR    L    R +  D      E +
Sbjct: 317 EEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV-----EYE 376

Query: 379 EYDIPKGHIIATSPAFAN--RLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRH 438
            Y IPKG  +   P F N      ++ DP ++DP RF      + A  P +++ FG G H
Sbjct: 377 GYLIPKGWKVL--PLFRNIHHNADIFSDPGKFDPSRF------EVAPKPNTFMPFGSGIH 436

Query: 439 GCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKG 474
            C G   A L+I  +   L   +   ++ P   I +    +   G
Sbjct: 437 SCPGNELAKLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNG 454

[Close]

BLAST of Chy7G143760 vs. TAIR 10
Match: AT5G45340.2 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 125.2 bits (313), Expect = 1.5e-28
Identity = 114/445 (25.62%), Postives = 194/445 (43.60%), Query Frame = 0


Query: 19  LLLAKLISALLNSRSNKRP-PPTVKGIPFIGGLLR-FLKGPIVMLRKEYPKLGSVFTVNL 78
           L L + I+ +  S S K P PP   G P++G   + + + P V    +  + GSVF  ++
Sbjct: 17  LCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLYSQDPNVFFAAKQRRYGSVFKTHV 76

Query: 79  LHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIRQEQFRFFTE 138
           L      +  PE +A F   ++S L +           G   +F        +  +    
Sbjct: 77  LGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRKLVLR 136

Query: 139 ALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILTASRCLLGSEVRDKLF- 198
           A     ++  V  +   A++  + W D  +++   E++      A   +LG   +D+++ 
Sbjct: 137 AFMPDAIRNMVPHIESIAQESLNSW-DGTQLNTYQEMKTYTFNVALISILG---KDEVYY 196

Query: 199 -ADVSALFHDLDNG--MLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASRKCNGSSD 258
             D+   ++ L+ G   +PI+     LP     +  +AR +LA I A I++ R+ N SS 
Sbjct: 197 REDLKRCYYILEKGYNSMPIN-----LPGTLFHKAMKARKELAQILANILSKRRQNPSSH 256

Query: 259 NDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHKEYMSAVL 318
            D+L  F++ K       TD ++   +I  +FA + T++   TW   YL  +   + AV 
Sbjct: 257 TDLLGSFMEDK----AGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLEAVT 316

Query: 319 EEQQ--RLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFSVTTREGK 378
           EEQ   R   K G+ +  +   +M   +R I+E LR    L    R +  D      E +
Sbjct: 317 EEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDV-----EYE 376

Query: 379 EYDIPKGHIIATSPAFAN--RLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYISFGGGRH 438
            Y IPKG  +   P F N      ++ DP ++DP RF      + A  P +++ FG G H
Sbjct: 377 GYLIPKGWKVL--PLFRNIHHNADIFSDPGKFDPSRF------EVAPKPNTFMPFGSGIH 434

Query: 439 GCLGEPFAYLQIKAIWSQLLRNFEL 454
            C G   A L+I  +   L   + L
Sbjct: 437 SCPGNELAKLEISVLIHHLTTKYRL 434

[Close]

BLAST of Chy7G143760 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 118.6 bits (296), Expect = 1.4e-26
Identity = 114/483 (23.60%), Postives = 203/483 (42.03%), Query Frame = 0


Query: 13  LLLLATLLLAKLISALLNSR---SNKRP-PPTVKGIPFIGGLLR-FLKGPIVMLRKEYPK 72
           L L A  L    +  L++ R   S+K P PP   G P++G   + + + P V  + +  +
Sbjct: 8   LTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKR 67

Query: 73  LGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIR 132
            GSVF  ++L      +  PE +A F   ++S L +           G   +F       
Sbjct: 68  YGSVFKTHVLGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYH 127

Query: 133 QEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILTASRCLLG 192
            +  +    A     ++  V  +   A+D    W    E  +    + +   T +  LL 
Sbjct: 128 AKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW----EGTMINTYQEMKTYTFNVALLS 187

Query: 193 SEVRDKLF--ADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASR 252
              +D++    D+   ++ L+ G   + V    LP     +  +AR +L+ I A+I++ R
Sbjct: 188 IFGKDEVLYREDLKRCYYILEKGYNSMPV---NLPGTLFHKSMKARKELSQILARILSER 247

Query: 253 KCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHK 312
           + NGSS ND+L  F+  K       TD ++   +I  +FA + T++   +W   YL  + 
Sbjct: 248 RQNGSSHNDLLGSFMGDK----EELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENP 307

Query: 313 EYMSAVLEEQQ--RLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFS 372
             + AV EEQ   R   + G+ +      +M    R I+E LR+   L    R +  D  
Sbjct: 308 NVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDV- 367

Query: 373 VTTREGKEYDIPKGHIIATSPAFAN--RLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 432
               E + Y IPKG  +   P F N      ++ +P ++DP RF      + A  P +++
Sbjct: 368 ----EYEGYLIPKGWKVL--PLFRNIHHSADIFSNPGKFDPSRF------EVAPKPNTFM 427

Query: 433 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNFELELISPFPEIDWNAMVVGVKGKVMVRYKR 485
            FG G H C G   A L++  +   L   +   ++     I +    +   G  +V  ++
Sbjct: 428 PFGNGTHSCPGNELAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPIVLARK 465

[Close]

BLAST of Chy7G143760 vs. TAIR 10
Match: AT4G19230.2 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 114.8 bits (286), Expect = 2.0e-25
Identity = 111/450 (24.67%), Postives = 191/450 (42.44%), Query Frame = 0


Query: 13  LLLLATLLLAKLISALLNSR---SNKRP-PPTVKGIPFIGGLLR-FLKGPIVMLRKEYPK 72
           L L A  L    +  L++ R   S+K P PP   G P++G   + + + P V  + +  +
Sbjct: 8   LTLFAGSLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLYSQDPNVFFQSKQKR 67

Query: 73  LGSVFTVNLLHKKITFLIGPEVSAHFFKASESDLSQQEVYQFNVPTFGPGVVFDVDYSIR 132
            GSVF  ++L      +  PE +A F   ++S L +           G   +F       
Sbjct: 68  YGSVFKTHVLGCPCVMISSPE-AAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYH 127

Query: 133 QEQFRFFTEALRVTKLKGYVDQMVREAEDFFSKWGDCGEVDLKYELEHLIILTASRCLLG 192
            +  +    A     ++  V  +   A+D    W    E  +    + +   T +  LL 
Sbjct: 128 AKLRKLVLRAFMPESIRNMVPDIESIAQDSLRSW----EGTMINTYQEMKTYTFNVALLS 187

Query: 193 SEVRDKLF--ADVSALFHDLDNGMLPISVMFPYLPIPAHRRRDQARSKLADIFAKIIASR 252
              +D++    D+   ++ L+ G   + V    LP     +  +AR +L+ I A+I++ R
Sbjct: 188 IFGKDEVLYREDLKRCYYILEKGYNSMPV---NLPGTLFHKSMKARKELSQILARILSER 247

Query: 253 KCNGSSDNDMLQCFIDSKYKDGRSTTDSEVTGLLIAALFAGQHTSSITSTWTGAYLLCHK 312
           + NGSS ND+L  F+  K       TD ++   +I  +FA + T++   +W   YL  + 
Sbjct: 248 RQNGSSHNDLLGSFMGDK----EELTDEQIADNIIGVIFAARDTTASVMSWILKYLAENP 307

Query: 313 EYMSAVLEEQQ--RLMGKHGDKIDHDILSEMDNLHRCIKEALRLHPPLIMLMRSSHSDFS 372
             + AV EEQ   R   + G+ +      +M    R I+E LR+   L    R +  D  
Sbjct: 308 NVLEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDV- 367

Query: 373 VTTREGKEYDIPKGHIIATSPAFAN--RLPHVYKDPDRYDPDRFAPGRDEDKAAGPFSYI 432
               E + Y IPKG  +   P F N      ++ +P ++DP RF      + A  P +++
Sbjct: 368 ----EYEGYLIPKGWKVL--PLFRNIHHSADIFSNPGKFDPSRF------EVAPKPNTFM 427

Query: 433 SFGGGRHGCLGEPFAYLQIKAIWSQLLRNF 452
            FG G H C G   A L++  +   L   +
Sbjct: 428 PFGNGTHSCPGNELAKLEMSIMIHHLTTKY 432

The following BLAST results are available for this feature:

BLAST of Chy7G143760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SAA9	7.4e-235	82.12	Sterol 14-demethylase OS=Arabidopsis thaliana OX=3702 GN=CYP51G1 PE=1 SV=1	[more]
P93596	6.7e-220	80.18	Obtusifoliol 14-alpha demethylase (Fragment) OS=Triticum aestivum OX=4565 GN=CYP...	[more]
P93846	4.4e-219	75.80	Obtusifoliol 14-alpha demethylase OS=Sorghum bicolor OX=4558 GN=CYP51 PE=1 SV=1	[more]
Q1ZXH9	1.6e-104	44.73	Probable lanosterol 14-alpha demethylase OS=Dictyostelium discoideum OX=44689 GN...	[more]
Q4PJW3	8.5e-90	38.57	Lanosterol 14-alpha demethylase OS=Bos taurus OX=9913 GN=CYP51A1 PE=2 SV=1	[more]

BLAST of Chy7G143760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KYP3	9.3e-281	99.18	Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa4G007710 PE=2 SV=1	[more]
A0A5A7TLX5	3.5e-280	99.18	Sterol 14-demethylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold65...	[more]
A0A1S3BXE7	3.5e-280	99.18	sterol 14-demethylase OS=Cucumis melo OX=3656 GN=LOC103494635 PE=3 SV=1	[more]
A0A6J1J0F8	4.8e-269	95.26	sterol 14-demethylase-like OS=Cucurbita maxima OX=3661 GN=LOC111480132 PE=3 SV=1	[more]
A0A6J1GUN6	5.3e-268	94.85	sterol 14-demethylase-like OS=Cucurbita moschata OX=3662 GN=LOC111457642 PE=3 SV...	[more]

BLAST of Chy7G143760 vs. NCBI nr


Match Name	Identity	Description
NP_001295809.1	99.18	sterol 14-demethylase [Cucumis sativus] >AIY67848.1 cytochrome P450 [Cucumis sat...	[more]
XP_008454145.1	99.18	PREDICTED: sterol 14-demethylase [Cucumis melo] >KAA0044503.1 sterol 14-demethyl...	[more]
XP_038882542.1	96.29	sterol 14-demethylase [Benincasa hispida]	[more]
XP_023527569.1	95.26	sterol 14-demethylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022980879.1	95.26	sterol 14-demethylase-like [Cucurbita maxima]	[more]

BLAST of Chy7G143760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11680.1	5.3e-236	82.12	CYTOCHROME P450 51G1	[more]
AT5G45340.1	6.0e-30	24.95	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT5G45340.2	1.5e-28	25.62	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]
AT4G19230.1	1.4e-26	23.60	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT4G19230.2	2.0e-25	24.67	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 421..430 score: 40.95 coord: 430..441 score: 38.9 coord: 339..350 score: 40.69 coord: 285..302 score: 32.57
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 38..457 e-value: 9.1E-66 score: 222.5
IPR002403	Cytochrome P450, E-class, group IV	PRINTS	PR00465	EP450IV	coord: 372..386 score: 37.18 coord: 388..406 score: 28.34 coord: 430..448 score: 43.52 coord: 276..302 score: 37.32 coord: 414..430 score: 34.39 coord: 37..54 score: 34.83 coord: 334..350 score: 46.61 coord: 59..82 score: 35.9
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 29..485 e-value: 1.7E-122 score: 411.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 33..482
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 11..482 e-value: 1.1E-35 score: 121.3
None	No IPR available	PANTHER	PTHR24286:SF251	STEROL 14-DEMETHYLASE	coord: 10..480
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 10..480
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 423..432

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy7G143760.1	Chy7G143760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0016126	sterol biosynthetic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen