Chy7G137210 (gene) Cucumber (hystrix) v1

Overview


Name	Chy7G137210
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	5'-nucleotidase domain-containing protein DDB_G0275467
Location	chrH07: 12488127 .. 12491157 (-)
RNA-Seq Expression	Chy7G137210
Synteny	Chy7G137210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGTTTTTCTCCATCTTTTATCATAACAACGCTTTGTTTCGTTTGTCTTTTTTGTTTCAACTTAGTTACCTCCTTTATATGGCTCTTTCACTACTATCCAGTCATGAAATAATCTGTCATGAAGTTAGTTAATTGCAGATCTAATAATAAAATCATTTTCAAACGGTAAAAAAACAAAAAAAAAAGTTCATGATAGCAAGATTTTTAGAGGGATAGTGACAGCACAATGTGACACAGAGTCTAGAAAACGTACAAATTTCAAAGGAATGGTAGTGTATCAATAATAGGGTGTAGATAAATGGTTATAGAAAAGTATTGTTGTGAAAGTATGATATTGTTACTATGTATCTCACCGATAATTCAAAGAAAGTTGTATATTTGAACTTTGGGATTGGAATGATTTTTTTAAGGGAAATAGGAATCATGGTGTCTTAATTATTTACTTCAATTACAACTAAATTGTGTATCGCAGGTCTATAATGAAGAGGTCACTTTCAGATAGCAATATTTTCTTAGGAAGTGAAATTCCTACGGCCTAACATGATAGTGAACTTCTGCACAAAAAATCGCAAAATAAAGGACTCGTAGAATTATTGCCAGAAATTTCAACCTATTGCAGGTATGCATCCTTTCTAAACATCTCAATTAATATTCACATTTAGAAAGTTTACAAGGATTCTTGGAGTTAATATTCATACAAATTTCTAAAATTTAGTTAAAGGTCTTGTTTCACATATAGAAAGTTTACATTATTGGTCTACTCTCAAATAAGAGTTCTTTTTTGGCGATTGTACACAATCTGTAGTCTTGCTTAGTTTTCTAGTTTTCTGGCAATATTTCTTCTCCTGTGTAGTCATATTTTTATATAAGTTTTTTTTTTCTATGGTTAATAGGCATGCTTTATTGCAGATGTTGTTATCGAGAGAATCTTCGTTGGGCGTTTACTATTTCACTGAAGTTTCAAACTTTAAAATATAATGATAACTTTTATTAATTAGTATAGTGTAATCATCATCTTGCGCATAAAATGTTCTCTTGTTATCAGGCTTCTATTGTTTCAAGGATTCTTGGAGAGAAATCTTTGATGTTGTGATAGCTAAGGCTAATATGCCAGAATTTTCCTCTGTCTATTGCATATAGAGTTTTTACTTTCCTATGTTTTCAGAGAGAAATGGAAGATATGATACTGATTGTTTTCTTTCTACTTATTCTCTAAAACATCAATATTAGTTGTTATGACACAGAAAAGGACACTTTAACTTTTACAAAGGTGGATGCATTCCTTCCAAATAAAATTTATTTCCATGGATGCCATAAGTCTTTCCTTCAAATTACCAAGTGGAATGGTCCTGAGGTAGTTTGATAAAAAGCATAATGTTTTGTTGTTATTTGGATGACATTGCTCTCACATGTTGTAGCCTGAGCATTATGTATTTGTTTCTTCCGACAAGGTTATATACTTTGGGGATCACCTTTTTAGCGATCTAAGAGGCCCTTCAAAAGCTAGTTGGTGCACTGCTGCTATCATTTATGAATTGAAGGTATGTATTATTTCTAGGTGCAACAAGGGGTGTAGTTTCAAGATTTGTGAATGGAATTTTATTCAATTTAAGAATCGATTTTATCGATTTTTTGTTTGAAGACATTATGGAATTTTTTAATAAGAGTTTTATTATGCAAGGTCGAAAGCGGTTGAACGATAAGCGATTGTTTGTGCTAGTAGAGTACCAATAATGCAGGTAGATGAACTTTCATCCATTGATGCACCAAAGATGGAAATTGCATCTGAGTATGGTATGGGCTGCTTCTCAATATCCACCTTATAAGTTTTTATATTCACCTCAAAAGCCAATGTGACAAATCAAGCCATCAACCCTTTCAACCTCTGCCCCACTTTCTAAGGAGAATCTGGTCGAATGCCATTTTCACTGTCCAATGATTTGGTGAGTTTGGGTAGGAGCCCTAAAATCTATCTTGTACACCATTCAATAATAATAAAGGTTTTTCTGATAGATCCTTGTAGTTGAGAACTTGAACACTCTGTCTTTCTAGTTTCCTTCAAATAAATGTCATCACCAATGTCTAAAACTAGAAAGTCTAATTGCATATATTCACAATGGTATGGTATTATTCACTTTCATCTCCAATCTTCAGTGTTTTATTCTTAGACTCTTTTGAAAGAGACGCACACCCATATTTCAATTGATGCACGTTTATGTTCACGTCCCTACTTATCAATGTGTAGGCGAAAATAGGAAACTAAACTACATATAGGAGAATTGTGGATTCATTTGTTTCATTAATTGGATGTTACTTTAATGCAAAGGACTTTGCTTGGAAGCATAGATGAGGAATTTGATGTTGAAGATTAAAAAGAAAACTGGTTGGAGCTTTTATAATAGAGTGAAGAACATGTATATAGAGGATTTTGATAACTCTATGCCCCTTTGTTAAATAGGGGAATTAAAAATGGAAAAGTTGGTTGTGAAAGATAGTGAAATGGCAACATCTAAATAAGTTTTTATATGAATGTGCCTTTCATTTTCTGTCAAGGAAAGCAAGATTTGTGCAAGATTTGTATTATTTCAAGATTTGTTCGATTTTATTCAAGTTACATAATGTCTTATCGTGTTTTTAATATTAAAAAAAAACGGGCACATAGTATTTTCTGTAACATTTTTCAGAGAAGTGAAGCTCAATGTTTGATCCTTCAGGACTATGGGATGAAAATGTGTCTTGTGCAAAGCAGCACATTTCATATGCTTATGATTTAGTTTGTTTTGTTTTACTTCTGCACTCTGCAGACTTAAGATATAATAGTGTAATTTTGTTAATTATTTTTTCAGATCCGAGCTTATTCTCTCAATAGCAATAAATCTCCATTTATTATTTGCTTTTGGCATTAAGTGATTAAGGTCAATGTGCATTGTTGTGATGTATCAGGATGATTTCTTGCCTCGGACCATTACAGCTTTGGAGGATGTATTCAAATCTCGTCTGGTAAACATACAGTTTTTTGTATTATGA

mRNA sequence

ATGGAGGTTATATACTTTGGGGATCACCTTTTTAGCGATCTAAGAGGCCCTTCAAAAGCTAGTTGGTGCACTGCTGCTATCATTTATGAATTGAAGGTAGATGAACTTTCATCCATTGATGCACCAAAGATGGAAATTGCATCTGAGTATGGACTATGGGATGAAAATGTGTCTTGTGCAAAGCAGCACATTTCATATGCTTATGATTTAGTTTGTTTTGTTTTACTTCTGCACTCTGCAGACTTAAGATATAATAGTGATGATTTCTTGCCTCGGACCATTACAGCTTTGGAGGATGTATTCAAATCTCGTCTGGTAAACATACAGTTTTTTGTATTATGA

Coding sequence (CDS)

ATGGAGGTTATATACTTTGGGGATCACCTTTTTAGCGATCTAAGAGGCCCTTCAAAAGCTAGTTGGTGCACTGCTGCTATCATTTATGAATTGAAGGTAGATGAACTTTCATCCATTGATGCACCAAAGATGGAAATTGCATCTGAGTATGGACTATGGGATGAAAATGTGTCTTGTGCAAAGCAGCACATTTCATATGCTTATGATTTAGTTTGTTTTGTTTTACTTCTGCACTCTGCAGACTTAAGATATAATAGTGATGATTTCTTGCCTCGGACCATTACAGCTTTGGAGGATGTATTCAAATCTCGTCTGGTAAACATACAGTTTTTTGTATTATGA

Protein sequence

MEVIYFGDHLFSDLRGPSKASWCTAAIIYELKVDELSSIDAPKMEIASEYGLWDENVSCAKQHISYAYDLVCFVLLLHSADLRYNSDDFLPRTITALEDVFKSRLVNIQFFVL*

Homology

[Close]

BLAST of Chy7G137210 vs. ExPASy TrEMBL
Match: A0A2P5FNG5 (Purine 5'-nucleotidase OS=Trema orientale OX=63057 GN=TorRG33x02_048260 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.9e-07
Identity = 41/88 (46.59%), Postives = 51/88 (57.95%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELKVD-ELSSIDAPKMEIAS----EYGLWDEN 61
           EVIYFGDHLFSDLRGPSKA W TAAII+EL+ +    + DA + E A     +  L   +
Sbjct: 395 EVIYFGDHLFSDLRGPSKAGWRTAAIIHELESEIRTQNEDAYRFEQAKFHIIQELLGKLH 454

Query: 62  VSCAKQHISYAYDLVCFVLLLHSADLRY 85
            + A  H S AY L+   L L     R+
Sbjct: 455 STVASNHQSKAYKLLLEELNLQRLKARH 482

[Close]

BLAST of Chy7G137210 vs. ExPASy TrEMBL
Match: A0A5A7T188 (5'-nucleotidase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold271G00280 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.4e-07
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELK 33
           EVIYFGDHLFSDLRGPSKA W TAAIIYEL+
Sbjct: 395 EVIYFGDHLFSDLRGPSKAGWRTAAIIYELE 425

[Close]

BLAST of Chy7G137210 vs. ExPASy TrEMBL
Match: A0A1S3UTP4 (5'-nucleotidase domain-containing protein DDB_G0275467 OS=Vigna radiata var. radiata OX=3916 GN=LOC106768617 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.4e-07
Identity = 30/47 (63.83%), Postives = 38/47 (80.85%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELKVD-ELSSIDAPKMEIA 48
           EVIYFGDHLFSDLRGPSKA W TAAII+EL+ + ++ + D  + E+A
Sbjct: 381 EVIYFGDHLFSDLRGPSKAGWRTAAIIHELESEIQMQNEDTYRSELA 427

[Close]

BLAST of Chy7G137210 vs. ExPASy TrEMBL
Match: A0A6J1JHK0 (LOW QUALITY PROTEIN: 5'-nucleotidase domain-containing protein DDB_G0275467 OS=Cucurbita maxima OX=3661 GN=LOC111485769 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.4e-07
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELK 33
           EVIYFGDHLFSDLRGPSKA W TAAIIYEL+
Sbjct: 395 EVIYFGDHLFSDLRGPSKAGWRTAAIIYELE 425

[Close]

BLAST of Chy7G137210 vs. ExPASy TrEMBL
Match: A0A067LFD2 (Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_00488 PE=3 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 6.4e-07
Identity = 37/72 (51.39%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELKV------DELSSIDAPKMEIASE-YGLWD 61
           EVIYFGDHLFSDLRGPSKA W TAAII+EL+       DE    +  K  I  E  G   
Sbjct: 396 EVIYFGDHLFSDLRGPSKAGWHTAAIIHELESEIHIQNDETYRFEQAKFHIIQELLGKLH 455

Query: 62  ENVSCAKQHISY 67
             V+  K+  SY
Sbjct: 456 STVANCKRSDSY 467

[Close]

BLAST of Chy7G137210 vs. NCBI nr
Match: XP_012066429.1 (5'-nucleotidase domain-containing protein DDB_G0275467 isoform X2 [Jatropha curcas])

HSP 1 Score: 65.1 bits (157), Expect = 1.83e-09
Identity = 29/34 (85.29%), Postives = 31/34 (91.18%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELKVDE 35
           EVIYFGDHLFSDLRGPSKA W TAAII+EL+ DE
Sbjct: 398 EVIYFGDHLFSDLRGPSKAGWHTAAIIHELEEDE 431

[Close]

BLAST of Chy7G137210 vs. NCBI nr
Match: PON99283.1 (Purine 5'-nucleotidase [Trema orientale])

HSP 1 Score: 63.9 bits (154), Expect = 4.78e-09
Identity = 40/88 (45.45%), Postives = 52/88 (59.09%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELKVD-ELSSIDAPKMEIASEYGLWDE----N 61
           EVIYFGDHLFSDLRGPSKA W TAAII+EL+ +    + DA + E A  + + +     +
Sbjct: 395 EVIYFGDHLFSDLRGPSKAGWRTAAIIHELESEIRTQNEDAYRFEQAKFHIIQELLGKLH 454

Query: 62  VSCAKQHISYAYDLVCFVLLLHSADLRY 84
            + A  H S AY L+   L L     R+
Sbjct: 455 STVASNHQSKAYKLLLEELNLQRLKARH 482

[Close]

BLAST of Chy7G137210 vs. NCBI nr
Match: XP_021856560.1 (5'-nucleotidase domain-containing protein DDB_G0275467 isoform X1 [Spinacia oleracea] >KNA19154.1 hypothetical protein SOVF_063970 isoform A [Spinacia oleracea])

HSP 1 Score: 63.5 bits (153), Expect = 6.50e-09
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELK 32
           EVIYFGDHLFSDLRGPSKA W TAAIIYEL+
Sbjct: 378 EVIYFGDHLFSDLRGPSKAGWRTAAIIYELE 408

[Close]

BLAST of Chy7G137210 vs. NCBI nr
Match: XP_021856561.1 (5'-nucleotidase domain-containing protein DDB_G0275467 isoform X2 [Spinacia oleracea] >KNA19155.1 hypothetical protein SOVF_063970 isoform B [Spinacia oleracea])

HSP 1 Score: 63.5 bits (153), Expect = 6.50e-09
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELK 32
           EVIYFGDHLFSDLRGPSKA W TAAIIYEL+
Sbjct: 378 EVIYFGDHLFSDLRGPSKAGWRTAAIIYELE 408

[Close]

BLAST of Chy7G137210 vs. NCBI nr
Match: XP_010687674.1 (PREDICTED: 5'-nucleotidase domain-containing protein DDB_G0275467 [Beta vulgaris subsp. vulgaris] >KMT03516.1 hypothetical protein BVRB_8g191940 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 63.5 bits (153), Expect = 6.51e-09
Identity = 28/31 (90.32%), Postives = 29/31 (93.55%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELK 32
           EVIYFGDHLFSDLRGPSKA W TAAIIYEL+
Sbjct: 380 EVIYFGDHLFSDLRGPSKAGWRTAAIIYELE 410

[Close]

BLAST of Chy7G137210 vs. TAIR 10
Match: AT2G23890.1 (HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase )

HSP 1 Score: 62.0 bits (149), Expect = 3.6e-10
Identity = 30/47 (63.83%), Postives = 38/47 (80.85%), Query Frame = 0


Query: 2   EVIYFGDHLFSDLRGPSKASWCTAAIIYELKVD-ELSSIDAPKMEIA 48
           EVIYFGDHLFSDLRGPSKA W TAAII+EL+ + ++ + D+ + E A
Sbjct: 393 EVIYFGDHLFSDLRGPSKAGWRTAAIIHELEREIQIQNDDSYRFEQA 439

The following BLAST results are available for this feature:

BLAST of Chy7G137210 vs.


Match Name	E-value	Identity	Description

BLAST of Chy7G137210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2P5FNG5	4.9e-07	46.59	Purine 5'-nucleotidase OS=Trema orientale OX=63057 GN=TorRG33x02_048260 PE=3 SV=...	[more]
A0A5A7T188	6.4e-07	90.32	5'-nucleotidase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3UTP4	6.4e-07	63.83	5'-nucleotidase domain-containing protein DDB_G0275467 OS=Vigna radiata var. rad...	[more]
A0A6J1JHK0	6.4e-07	90.32	LOW QUALITY PROTEIN: 5'-nucleotidase domain-containing protein DDB_G0275467 OS=C...	[more]
A0A067LFD2	6.4e-07	51.39	Uncharacterized protein OS=Jatropha curcas OX=180498 GN=JCGZ_00488 PE=3 SV=1	[more]

BLAST of Chy7G137210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_012066429.1	1.83e-09	85.29	5'-nucleotidase domain-containing protein DDB_G0275467 isoform X2 [Jatropha curc...	[more]
PON99283.1	4.78e-09	45.45	Purine 5'-nucleotidase [Trema orientale]	[more]
XP_021856560.1	6.50e-09	90.32	5'-nucleotidase domain-containing protein DDB_G0275467 isoform X1 [Spinacia oler...	[more]
XP_021856561.1	6.50e-09	90.32	5'-nucleotidase domain-containing protein DDB_G0275467 isoform X2 [Spinacia oler...	[more]
XP_010687674.1	6.51e-09	90.32	PREDICTED: 5'-nucleotidase domain-containing protein DDB_G0275467 [Beta vulgaris...	[more]

BLAST of Chy7G137210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23890.1	3.6e-10	63.83	HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008380	HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase	PFAM	PF05761	5_nucleotid	coord: 2..43 e-value: 6.7E-7 score: 28.5
IPR023214	HAD superfamily	GENE3D	3.40.50.1000		coord: 1..52 e-value: 5.4E-6 score: 27.7
IPR036412	HAD-like superfamily	SUPERFAMILY	56784	HAD-like	coord: 2..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy7G137210.1	Chy7G137210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity
molecular_function	GO:0046872	metal ion binding