Chy7G130840 (gene) Cucumber (hystrix) v1

Overview
NameChy7G130840
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionProtein of unknown function (DUF803)
LocationchrH07: 2001104 .. 2005097 (-)
RNA-Seq ExpressionChy7G130840
SyntenyChy7G130840
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGAAGGGAATGATGGGAAAACGTGGGGTCAAAATTGGAGAACGAAAGCGAGTAGAGCTTGGGATATTCTTAATCTTGCTTTTCTTATTGCTTGGCTGACTTTTTGGAATTAATTTTCGGTGGCGTGCTCCTGTGCACTTTGTAATAAATTTTTAAGAGATTTTACCACTGATTTGTTGTTGTTGTCGTTGATCATATGCATTTCAATTTGTTGATTATGGTCAACTTTGCTGTTTGGAAGAGGATGAATTAGCTTGACTTCCACTGTGTTATTTTAAAAGAGAGAGAAAAAAAAAAAAAAACAAGTAGTGTTTTTTATTCTCAATTTTCTATATTTCACAAACATCATTGTTTAAGCAAATTGCGAAAATCATTATTAGAACTGCATATTATATTTGATTATATAAGTTTAATTTTAACCTTTTTTTTTAGGGTAAGATCTAATCGCTATTTTTTAAAATTTAAGAGTGTAATTACAACTATTCCAGTATTTTATTAATAGTTTAAAAATATTTTTTTTACAACTTGAATTATATTAGTTAGTAAGAGTGTCTAATAAGTTATTACTCTCAATGTATTCATATAAATTAACATTTTCTTCATCTACGAATTTAGTAGCCATTCATTTAAAGGTTGGTAAACTTGTGGTAGATAATTTGATTAGAGCTTTATAACCTTTTTTTTTATGCAAAATTAACCATTAATATATATAGAGTCACTTGTGATTTTGAGTTTTGGTGAATCATATTGATAGATTAGTTTAATTGAATTAATTTTATTTGGTTTTGTGTCAATAAAAATAATTGACAAAATTAAAATAGAAAAATTAATTTCAGTTCGATTTAATTTTAAATTAAAAAAACCGAATTAAACCACCAAACCGACCAGTGATTATTATATTGGTTTCAATTTGAGATAAAAAAACGGTTCGGTTCGGTTGAACTGGCAACTCTAATTAAAGGGCGGAAAAAGTTTAGCCAATAAAAAGGTAGCACGTAGCAGGTAAAAAAAGATGAAGGAACATATGTGAGTCGCACGTGATTAGATCCCATGGGCTTTGTTGGTGGGCCTGCTTTCGTTAGAAATACAAAGAGAGAAAGAAGAAGTCGAAAGGACAAAATTCTGTCGTTGGCGCAAAAAATTTCGAGGCAATGTTCTTCTAACGTAAATCCTAATTCTCCCTTAAATTTCTTGATTTCTGCTTCTCAATCTCTTTCCTTCTTTTGTTTTCTGCGTTTCGATTTTTAAAGAAGATATGTGGGAACCGATTTGCTTAACTTTGGCCGCCGCGGCCGGCAACAATATTGGCAAAATACTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTTCGCTTTCTCTTTTTCTTTCTCTTACCTTTGTTTGTTTGTTTGTTTTTTTTTTTCACATTTTCTTCTAGGTCTTTTAATTTCATGCGATTCGTTTTCTTATTCTTCCTTGAGCTGTCCGATCGCTTGCAGTATGGTGTTTCTTTGATTCTCTAATTTTTCGGATTTCATTAAAGATTTGAGCGAATTTTATTTTCGCTTTCTTCTGGTGAATTCCTTGAATGAAGTTGTGAATTCTCATTGGTTTTTTGTGTTGTATAGGTGATAAGGGCTTACGCTTTTAACAAAACTTGGATCATTGGTTTTCTGATGGATATATTTGGAGCCGTTTTAATGTTGAGAGCGCTATCCCTCGCCCCTGTAAGCTATTTTATTCAACTACATACGCCATTTTCTTTTATGTTTTTTGATTGGCATTCGATGATTGGATCTTAGGCATTATTGAGCGAATATGCTGTTTTTGTTCTGAGATCTAGATGAATTTAGCGCGCCTTTATAGTCTCCCTACAGGCTACATTTGATGGAAAATATCAATTGCTGTTGTGATGGCTAACTGTCTCCTCTTTCATTCAGCATCGCCTTTATAGTCGTATTTATTATATCGTATTTATCTCTATGAAAATTACATATAATCTACGATTCTAAATATTTCATAGATTTTTTTCCCAAGAATCCGTACCTCACAGGAAAATTTGTATGTTGCGCACATTTTCGTCTCTAATATGCATTTCCTTTCTAATTTTTGAATTTCAGGTTTCTATAATTCAACCAGTTTCTGGTTGTGGACTCGCCATCCTCTCTATATTTTCACATTTTTATCTGAAGGAAATTATGAATGTTGTTGATTGGATGGGGATTATTCTTGCTGGTATTGGCACCATAGGTAATGTAAGTTCATCTTTGTGTCTTTATTAGTTTAGTTATTGTTTGATCTCTTGATCTCTATTTTATGCATTTCAGGTTTGGCCATGCCTTGTATGCCAAGTTTTTATTGATCTTCATCTTCCTTGCACACATTTGTAATTTATTTGGCTATTTTCTTTTTTCTCCCTTTTTTCACCATTTCTAGGGGTTGGTGCTGGAGGCGAGGAGCAAAAGGCCTCTGCAATATCAGTTTTTCATTTGCCATGGCTAGCATTCATCATGACCATCTTGTTTGTAGGTATCTTATATTATTATAACCCCACGTTTTATAACAATCTCTGGTCATTTATGTTAGAAGTTTTATGGGCTTGCTTGCAGAAACAAATATAACATACTAGTACTTTGTAAATAAGGCTGTAAAAGTTGAGTGAATTGACATCTAGAAATGATTGGCTACACATATTTTTAACAGGTTCTTCTAAATGGCTGGCTTCATTTCTACAAACGTCAAAGGAGAGAACAAGAGTTGGTAAATTCATTGCTTTTATATTGACATTTCAATTGTTTTCTTTTTCCTCCCAGAAAAATTATATGTTACATATGTATATATGTTGCAGATGGAATTCGAAGTTGTTGAAGAAATAATATATGGCTTGGAATCTGGCATTTTGTTTGGGTACTTTTCTTGTTTGCACTCTTTTACTTGATGGATTACATTTCCTTAAATCATTAAGGTTTTAGAGGATGACTATTAGGAATTAGAACTTTGCTTTACTGTGAGGAATTGGGGCTTCTGCCTGACTGTGGTGGTATCCTAGTTGTTTCAAATTGGATCTTAGCCTGCTTGTATTTTGACCTTCCAGTTGTACTAGGCATTGAAAAGTAGAATTTTGAATAATCGTTGTTGTGGTTTTCTGGAACATAAGCATACCATAGAATTGTTAGTTTAGTCTTTTACATATATGGCATAAATATAGAATGGAAATAATCCAATTGGCCTTTTAGATTCATAATAGCAAAATTCACAAGTTAAATCTGCAATTGCAGGATGGCGTCTGTAATATCAAAGATGGGATTCTTATTCTTGGAGCAGGGTTTCCACCAAATATTGGTTCCCATATGCATCTTGATAAGTATTTGTTGCAGCGCTACAGGATTTTACTATCAGGTACTTCAAATTCTTGGTGATTTGATCTCTTTACGACTCTATTTTCACTCGGTGTTCGTTGTTTTATCAACTTTTCTCTGTGTTCTTCTAGTTCATTGTCTTCTTTTTTAGTAGACTCGGGGACTAAAACATGGGAGGGCAATTGTGGTTTCTACATGTGCTGCGGTGGCATCCATCGTCACTGGTGTCCTTGCTGGCATGCTCGCCCTGGGTGAAGAATTGCCCTCGTCACCAACTGGTCGCCTTTTCCTTCTGCTGGGATGGTAAACTCCCTTACCTATAATTCCCTTTCGAGTTTACAATGACTGACCAGACTTGATAAAGAGTGGTTTCTGACCATGTGAATGTGTTCATTCATCTTCCTCTGTACAGGCTGCTAATCATCATAGGTGTTATTCTGCTCGTGAGTTCCTCACGACTGATCCGACGCGTTACTTGGCTATACCGGCGCTTTAAGCGAAGTGGTGTTGATAGGAACTATGGGCATAGATCTGGATCAGCTGTCCGCTTAAGAGACTCAAGCCCAAGTGCAATCATTCAGACAACAACGTTACAAAATTTGCTGTCTTCAAAAGCAAAGGCTGACGCTTGA

mRNA sequence

ATGGGGGAAGGGAATGATGGGAAAACGTGGGGTCAAAATTGGAGAACGAAAGCGAAAGATATGTGGGAACCGATTTGCTTAACTTTGGCCGCCGCGGCCGGCAACAATATTGGCAAAATACTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTGATAAGGGCTTACGCTTTTAACAAAACTTGGATCATTGGTTTTCTGATGGATATATTTGGAGCCGTTTTAATGTTGAGAGCGCTATCCCTCGCCCCTGTTTCTATAATTCAACCAGTTTCTGGTTGTGGACTCGCCATCCTCTCTATATTTTCACATTTTTATCTGAAGGAAATTATGAATGTTGTTGATTGGATGGGGATTATTCTTGCTGGTATTGGCACCATAGGGGTTGGTGCTGGAGGCGAGGAGCAAAAGGCCTCTGCAATATCAGTTTTTCATTTGCCATGGCTAGCATTCATCATGACCATCTTGTTTGTTCTTCTAAATGGCTGGCTTCATTTCTACAAACGTCAAAGGAGAGAACAAGAGTTGATGGAATTCGAAGTTGTTGAAGAAATAATATATGGCTTGGAATCTGGCATTTTGTTTGGGATGGCGTCTGTAATATCAAAGATGGGATTCTTATTCTTGGAGCAGGGTTTCCACCAAATATTGGTTCCCATATGCATCTTGATAAGTATTTGTTGCAGCGCTACAGGATTTTACTATCAGACTCGGGGACTAAAACATGGGAGGGCAATTGTGGTTTCTACATGTGCTGCGGTGGCATCCATCGTCACTGGTGTCCTTGCTGGCATGCTCGCCCTGGGTGAAGAATTGCCCTCGTCACCAACTGGTCGCCTTTTCCTTCTGCTGGGATGGCTGCTAATCATCATAGGTGTTATTCTGCTCGTGAGTTCCTCACGACTGATCCGACGCGTTACTTGGCTATACCGGCGCTTTAAGCGAAGTGGTGTTGATAGGAACTATGGGCATAGATCTGGATCAGCTGTCCGCTTAAGAGACTCAAGCCCAAGTGCAATCATTCAGACAACAACGTTACAAAATTTGCTGTCTTCAAAAGCAAAGGCTGACGCTTGA

Coding sequence (CDS)

ATGGGGGAAGGGAATGATGGGAAAACGTGGGGTCAAAATTGGAGAACGAAAGCGAAAGATATGTGGGAACCGATTTGCTTAACTTTGGCCGCCGCGGCCGGCAACAATATTGGCAAAATACTTCAGAAGAAGGGCACCGTCATTCTTCCTCCTCTCTCTTTCAAGCTCAAGGTGATAAGGGCTTACGCTTTTAACAAAACTTGGATCATTGGTTTTCTGATGGATATATTTGGAGCCGTTTTAATGTTGAGAGCGCTATCCCTCGCCCCTGTTTCTATAATTCAACCAGTTTCTGGTTGTGGACTCGCCATCCTCTCTATATTTTCACATTTTTATCTGAAGGAAATTATGAATGTTGTTGATTGGATGGGGATTATTCTTGCTGGTATTGGCACCATAGGGGTTGGTGCTGGAGGCGAGGAGCAAAAGGCCTCTGCAATATCAGTTTTTCATTTGCCATGGCTAGCATTCATCATGACCATCTTGTTTGTTCTTCTAAATGGCTGGCTTCATTTCTACAAACGTCAAAGGAGAGAACAAGAGTTGATGGAATTCGAAGTTGTTGAAGAAATAATATATGGCTTGGAATCTGGCATTTTGTTTGGGATGGCGTCTGTAATATCAAAGATGGGATTCTTATTCTTGGAGCAGGGTTTCCACCAAATATTGGTTCCCATATGCATCTTGATAAGTATTTGTTGCAGCGCTACAGGATTTTACTATCAGACTCGGGGACTAAAACATGGGAGGGCAATTGTGGTTTCTACATGTGCTGCGGTGGCATCCATCGTCACTGGTGTCCTTGCTGGCATGCTCGCCCTGGGTGAAGAATTGCCCTCGTCACCAACTGGTCGCCTTTTCCTTCTGCTGGGATGGCTGCTAATCATCATAGGTGTTATTCTGCTCGTGAGTTCCTCACGACTGATCCGACGCGTTACTTGGCTATACCGGCGCTTTAAGCGAAGTGGTGTTGATAGGAACTATGGGCATAGATCTGGATCAGCTGTCCGCTTAAGAGACTCAAGCCCAAGTGCAATCATTCAGACAACAACGTTACAAAATTTGCTGTCTTCAAAAGCAAAGGCTGACGCTTGA

Protein sequence

MGEGNDGKTWGQNWRTKAKDMWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAVLMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFKRSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA*
Homology
BLAST of Chy7G130840 vs. ExPASy Swiss-Prot
Match: Q8RWH8 (Probable magnesium transporter NIPA9 OS=Arabidopsis thaliana OX=3702 GN=At5g11960 PE=2 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 4.7e-141
Identity = 252/341 (73.90%), Postives = 299/341 (87.68%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWE ICLTLAA AGNNIGK+LQKKGT+ILPPLS KLKV+RAYA NK W +GFLMDI GA+
Sbjct: 1   MWESICLTLAATAGNNIGKVLQKKGTIILPPLSLKLKVLRAYAENKPWALGFLMDIVGAL 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVS++QPVSGCGLAILS+FSHFYLKE+MNV DW+GI +AGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSVVQPVSGCGLAILSVFSHFYLKEVMNVFDWIGITVAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQ+AS ISVF L WLA ++ ILFVLLN WLH +KRQRREQEL E+EVVEEIIYGLESGIL
Sbjct: 121 EQEASLISVFQLLWLALVVAILFVLLNAWLHIFKRQRREQELGEYEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASV+SKMGF+F+EQGF  + +P+CI ISICCS TGF+YQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVVSKMGFVFVEQGFSTMFIPMCISISICCSGTGFFYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASIVTGV+AGM ALGE+LP+SP+GRL LLLGWLLI++GV+LLV+SSRLIR +   +RR +
Sbjct: 241 ASIVTGVVAGMFALGEKLPTSPSGRLLLLLGWLLIMLGVVLLVTSSRLIRHLPRSFRRSR 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAK 362
           ++ ++R +  R  ++   +D++PSA+IQ  TL +LLSS +K
Sbjct: 301 QTSLERGFNIRRTTSHTPKDTNPSAVIQAATLHHLLSSPSK 341

BLAST of Chy7G130840 vs. ExPASy Swiss-Prot
Match: P55580 (Uncharacterized protein y4nH OS=Sinorhizobium fredii (strain NBRC 101917 / NGR234) OX=394 GN=NGR_a02340 PE=4 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 3.7e-05
Identity = 37/117 (31.62%), Postives = 55/117 (47.01%), Query Frame = 0

Query: 24  PICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAVLML 83
           PI L  AA   + IG IL K G   LPP +          F    + G +      +L  
Sbjct: 5   PIILVFAAGLNSCIGNILLKWGRASLPPSAGLADTFLTPGF----VGGVVFYGINVLLFA 64

Query: 84  RALSLAPVSIIQPV-SGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGG 140
           +AL    VS+  P+ +G G A+L I SH++  E  ++  W+G+ L  +G I +  GG
Sbjct: 65  KALDSLEVSVAYPILAGSGFAMLIIASHYFFGEPFHLHKWIGVALVLVGIIFLARGG 117

BLAST of Chy7G130840 vs. ExPASy Swiss-Prot
Match: Q8BGN5 (NIPA-like protein 3 OS=Mus musculus OX=10090 GN=Nipal3 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 2.4e-04
Identity = 65/268 (24.25%), Postives = 114/268 (42.54%), Query Frame = 0

Query: 60  RAYAFNKTWIIGFLMDIFGAVLMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN- 119
           RAY   KTW +G L+ + G + +  + + AP+S+I P+S   +   +I    ++KE    
Sbjct: 72  RAYFKTKTWWLGLLLLLLGELGVFASYAFAPLSLIVPLSAVSVIASAIIGIIFIKEKWKP 131

Query: 120 -------VVDWMGIILAGIGT---IGVGAGGEEQKASAISVFHL---PWLAFIMT--ILF 179
                  V+ ++G  LA +GT   +       E+        HL   P+L +++   +LF
Sbjct: 132 KDFVRRYVLSFVGCGLAIVGTYLLVTFAPNSHEKMTGENIARHLVSWPFLLYMLVAIVLF 191

Query: 180 VLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQIL 239
            LL   L+FYK +          VV  ++  L   +       +S M  L + QG  Q+ 
Sbjct: 192 CLL---LYFYKERNANS-----IVVILLLVALLGSMTVVTVKAVSGMLVLSI-QGNLQLD 251

Query: 240 VPICILISICCSATGFYYQTRGLKHGRAI----VVSTCAAVASIVTGVLAGMLALGEELP 299
            PI  ++ +C  AT   YQ   L     I    ++++   + S    + AG +   + L 
Sbjct: 252 YPIFYVMFVCMVATAI-YQATFLSEASQIYDSSLIASVGYILSTTAAITAGAIFYLDFLG 311

Query: 300 SSPTGRLFLLLGWLLIIIGVILLVSSSR 308
                     LG L+  +GV L+  + +
Sbjct: 312 EEALHICMFALGCLIAFLGVFLITRNRK 329

BLAST of Chy7G130840 vs. ExPASy TrEMBL
Match: A0A0A0L5B6 (Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_4G646200 PE=3 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 3.2e-185
Identity = 341/344 (99.13%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRR+TWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 365
           RSGVDRN+GHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of Chy7G130840 vs. ExPASy TrEMBL
Match: A0A5A7VA96 (Probable magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G002250 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.6e-184
Identity = 339/344 (98.55%), Postives = 343/344 (99.71%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGILLAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRR+TWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 365
           R+GVDRN+GHRSGS VRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RNGVDRNFGHRSGSGVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of Chy7G130840 vs. ExPASy TrEMBL
Match: A0A1S3BUH9 (Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103493671 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.6e-184
Identity = 339/344 (98.55%), Postives = 343/344 (99.71%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGILLAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRR+TWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 365
           R+GVDRN+GHRSGS VRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RNGVDRNFGHRSGSGVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of Chy7G130840 vs. ExPASy TrEMBL
Match: A0A6J1FDL9 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111443049 PE=3 SV=1)

HSP 1 Score: 631.3 bits (1627), Expect = 2.5e-177
Identity = 326/345 (94.49%), Postives = 339/345 (98.26%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGKI+QKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKIIQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGI LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGITLAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQKASAIS+FHLPWLAFI+TILFVLL+GWLHFYKRQR+EQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISIFHLPWLAFIITILFVLLSGWLHFYKRQRKEQELMEFEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASVISKMGFLFLEQGFH+ILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASIVTGV+AGMLALGEELPSSPT RLFLLLGWLLII GVILLVSS+RL RR+TWLYRRFK
Sbjct: 241 ASIVTGVVAGMLALGEELPSSPTARLFLLLGWLLIITGVILLVSSARLTRRLTWLYRRFK 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQN-LLSSKAKADA 365
           RSGVDR++GHRSGS+VRLRDSSPSAIIQTTTLQN +LSSKAKADA
Sbjct: 301 RSGVDRSFGHRSGSSVRLRDSSPSAIIQTTTLQNMMLSSKAKADA 345

BLAST of Chy7G130840 vs. ExPASy TrEMBL
Match: A0A6J1FKB2 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111446202 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 4.2e-177
Identity = 321/344 (93.31%), Postives = 336/344 (97.67%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGK+LQKKGTVILPPLSFKLKVIRAYAFN+ WIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKVLQKKGTVILPPLSFKLKVIRAYAFNRAWIIGFLMDIFGAV 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN+VDWMGI LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNIVDWMGITLAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQKAS IS+F LPWLAF +TILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASEISIFRLPWLAFFITILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASVISK GFLFLEQGFH+ILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKTGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASI+TGV+AGMLALGEELPSSPT RLFLLLGWLLII+GVILLVSSSRLIRR+TWLYRRFK
Sbjct: 241 ASIITGVVAGMLALGEELPSSPTARLFLLLGWLLIIVGVILLVSSSRLIRRLTWLYRRFK 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 365
           +SG DRN+GHRSGS+VR+RDSSPSAIIQTTTLQNLLSSKAKAD+
Sbjct: 301 QSGTDRNFGHRSGSSVRIRDSSPSAIIQTTTLQNLLSSKAKADS 344

BLAST of Chy7G130840 vs. NCBI nr
Match: XP_004141325.1 (probable magnesium transporter NIPA9 isoform X2 [Cucumis sativus] >KGN55346.1 hypothetical protein Csa_012133 [Cucumis sativus])

HSP 1 Score: 656 bits (1693), Expect = 8.55e-237
Identity = 341/344 (99.13%), Postives = 344/344 (100.00%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRR+TWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 364
           RSGVDRN+GHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RSGVDRNFGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of Chy7G130840 vs. NCBI nr
Match: XP_008452740.1 (PREDICTED: probable magnesium transporter NIPA9 [Cucumis melo] >KAA0064508.1 putative magnesium transporter NIPA9 [Cucumis melo var. makuwa])

HSP 1 Score: 654 bits (1687), Expect = 7.02e-236
Identity = 339/344 (98.55%), Postives = 343/344 (99.71%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGILLAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRR+TWLYRRFK
Sbjct: 241 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRLTWLYRRFK 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAKADA 364
           R+GVDRN+GHRSGS VRLRDSSPSAIIQTTTLQNLLSSKAKADA
Sbjct: 301 RNGVDRNFGHRSGSGVRLRDSSPSAIIQTTTLQNLLSSKAKADA 344

BLAST of Chy7G130840 vs. NCBI nr
Match: XP_038896971.1 (probable magnesium transporter NIPA9 [Benincasa hispida])

HSP 1 Score: 642 bits (1655), Expect = 5.48e-231
Identity = 332/345 (96.23%), Postives = 341/345 (98.84%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGA+
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAI 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQKASAISVFHLPWLAFIMTILF+LLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISVFHLPWLAFIMTILFILLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASVISKMGFLFLEQGFH+ILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASI+TGV+AGMLALGEELPSSPTGRL LLLGWLLII+GVILLVSSSRLIRR TWLYRRFK
Sbjct: 241 ASIITGVVAGMLALGEELPSSPTGRLLLLLGWLLIIVGVILLVSSSRLIRRFTWLYRRFK 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNL-LSSKAKADA 364
           RSGVDRN+GHRSGS+VRLRDSSPSAIIQTTTLQNL LSSK KADA
Sbjct: 301 RSGVDRNFGHRSGSSVRLRDSSPSAIIQTTTLQNLMLSSKEKADA 345

BLAST of Chy7G130840 vs. NCBI nr
Match: XP_031740158.1 (probable magnesium transporter NIPA9 isoform X1 [Cucumis sativus])

HSP 1 Score: 640 bits (1650), Expect = 1.00e-229
Identity = 341/376 (90.69%), Postives = 344/376 (91.49%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIG------ 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGI+LAGIGTIG      
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIMLAGIGTIGNVWPCL 120

Query: 141 --------------------------VGAGGEEQKASAISVFHLPWLAFIMTILFVLLNG 200
                                     VGAGGEEQKASAISVFHLPWLAFIMTILFVLLNG
Sbjct: 121 VCQVFINIHLPCTHLQFTWLFSPFLGVGAGGEEQKASAISVFHLPWLAFIMTILFVLLNG 180

Query: 201 WLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICI 260
           WLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICI
Sbjct: 181 WLHFYKRQRREQELMEFEVVEEIIYGLESGILFGMASVISKMGFLFLEQGFHQILVPICI 240

Query: 261 LISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVLAGMLALGEELPSSPTGRLFL 320
           LISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVLAGMLALGEELPSSPTGRLFL
Sbjct: 241 LISICCSATGFYYQTRGLKHGRAIVVSTCAAVASIVTGVLAGMLALGEELPSSPTGRLFL 300

Query: 321 LLGWLLIIIGVILLVSSSRLIRRVTWLYRRFKRSGVDRNYGHRSGSAVRLRDSSPSAIIQ 364
           LLGWLLIIIGVILLVSSSRLIRR+TWLYRRFKRSGVDRN+GHRSGSAVRLRDSSPSAIIQ
Sbjct: 301 LLGWLLIIIGVILLVSSSRLIRRLTWLYRRFKRSGVDRNFGHRSGSAVRLRDSSPSAIIQ 360

BLAST of Chy7G130840 vs. NCBI nr
Match: XP_022936425.1 (probable magnesium transporter NIPA9 [Cucurbita moschata] >XP_023535010.1 probable magnesium transporter NIPA9 [Cucurbita pepo subsp. pepo] >KAG6591472.1 putative magnesium transporter NIPA9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 630 bits (1625), Expect = 2.04e-226
Identity = 326/345 (94.49%), Postives = 339/345 (98.26%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWEPICLTLAAAAGNNIGKI+QKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV
Sbjct: 1   MWEPICLTLAAAAGNNIGKIIQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMN VDWMGI LAGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNAVDWMGITLAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQKASAIS+FHLPWLAFI+TILFVLL+GWLHFYKRQR+EQELMEFEVVEEIIYGLESGIL
Sbjct: 121 EQKASAISIFHLPWLAFIITILFVLLSGWLHFYKRQRKEQELMEFEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASVISKMGFLFLEQGFH+ILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVISKMGFLFLEQGFHEILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASIVTGV+AGMLALGEELPSSPT RLFLLLGWLLII GVILLVSS+RL RR+TWLYRRFK
Sbjct: 241 ASIVTGVVAGMLALGEELPSSPTARLFLLLGWLLIITGVILLVSSARLTRRLTWLYRRFK 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNL-LSSKAKADA 364
           RSGVDR++GHRSGS+VRLRDSSPSAIIQTTTLQN+ LSSKAKADA
Sbjct: 301 RSGVDRSFGHRSGSSVRLRDSSPSAIIQTTTLQNMMLSSKAKADA 345

BLAST of Chy7G130840 vs. TAIR 10
Match: AT5G11960.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 502.3 bits (1292), Expect = 3.3e-142
Identity = 252/341 (73.90%), Postives = 299/341 (87.68%), Query Frame = 0

Query: 21  MWEPICLTLAAAAGNNIGKILQKKGTVILPPLSFKLKVIRAYAFNKTWIIGFLMDIFGAV 80
           MWE ICLTLAA AGNNIGK+LQKKGT+ILPPLS KLKV+RAYA NK W +GFLMDI GA+
Sbjct: 1   MWESICLTLAATAGNNIGKVLQKKGTIILPPLSLKLKVLRAYAENKPWALGFLMDIVGAL 60

Query: 81  LMLRALSLAPVSIIQPVSGCGLAILSIFSHFYLKEIMNVVDWMGIILAGIGTIGVGAGGE 140
           LMLRALSLAPVS++QPVSGCGLAILS+FSHFYLKE+MNV DW+GI +AGIGTIGVGAGGE
Sbjct: 61  LMLRALSLAPVSVVQPVSGCGLAILSVFSHFYLKEVMNVFDWIGITVAGIGTIGVGAGGE 120

Query: 141 EQKASAISVFHLPWLAFIMTILFVLLNGWLHFYKRQRREQELMEFEVVEEIIYGLESGIL 200
           EQ+AS ISVF L WLA ++ ILFVLLN WLH +KRQRREQEL E+EVVEEIIYGLESGIL
Sbjct: 121 EQEASLISVFQLLWLALVVAILFVLLNAWLHIFKRQRREQELGEYEVVEEIIYGLESGIL 180

Query: 201 FGMASVISKMGFLFLEQGFHQILVPICILISICCSATGFYYQTRGLKHGRAIVVSTCAAV 260
           FGMASV+SKMGF+F+EQGF  + +P+CI ISICCS TGF+YQTRGLKHGRAIVVSTCAAV
Sbjct: 181 FGMASVVSKMGFVFVEQGFSTMFIPMCISISICCSGTGFFYQTRGLKHGRAIVVSTCAAV 240

Query: 261 ASIVTGVLAGMLALGEELPSSPTGRLFLLLGWLLIIIGVILLVSSSRLIRRVTWLYRRFK 320
           ASIVTGV+AGM ALGE+LP+SP+GRL LLLGWLLI++GV+LLV+SSRLIR +   +RR +
Sbjct: 241 ASIVTGVVAGMFALGEKLPTSPSGRLLLLLGWLLIMLGVVLLVTSSRLIRHLPRSFRRSR 300

Query: 321 RSGVDRNYGHRSGSAVRLRDSSPSAIIQTTTLQNLLSSKAK 362
           ++ ++R +  R  ++   +D++PSA+IQ  TL +LLSS +K
Sbjct: 301 QTSLERGFNIRRTTSHTPKDTNPSAVIQAATLHHLLSSPSK 341

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8RWH84.7e-14173.90Probable magnesium transporter NIPA9 OS=Arabidopsis thaliana OX=3702 GN=At5g1196... [more]
P555803.7e-0531.62Uncharacterized protein y4nH OS=Sinorhizobium fredii (strain NBRC 101917 / NGR23... [more]
Q8BGN52.4e-0424.25NIPA-like protein 3 OS=Mus musculus OX=10090 GN=Nipal3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0L5B63.2e-18599.13Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_4G646200 PE=3 S... [more]
A0A5A7VA961.6e-18498.55Probable magnesium transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_s... [more]
A0A1S3BUH91.6e-18498.55Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103493671 PE=3 SV=1[more]
A0A6J1FDL92.5e-17794.49Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111443049 PE=... [more]
A0A6J1FKB24.2e-17793.31Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111446202 PE=... [more]
Match NameE-valueIdentityDescription
XP_004141325.18.55e-23799.13probable magnesium transporter NIPA9 isoform X2 [Cucumis sativus] >KGN55346.1 hy... [more]
XP_008452740.17.02e-23698.55PREDICTED: probable magnesium transporter NIPA9 [Cucumis melo] >KAA0064508.1 put... [more]
XP_038896971.15.48e-23196.23probable magnesium transporter NIPA9 [Benincasa hispida][more]
XP_031740158.11.00e-22990.69probable magnesium transporter NIPA9 isoform X1 [Cucumis sativus][more]
XP_022936425.12.04e-22694.49probable magnesium transporter NIPA9 [Cucurbita moschata] >XP_023535010.1 probab... [more]
Match NameE-valueIdentityDescription
AT5G11960.13.3e-14273.90Protein of unknown function (DUF803) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008521Magnesium transporter NIPAPFAMPF05653Mg_trans_NIPAcoord: 60..303
e-value: 3.2E-8
score: 33.1
IPR008521Magnesium transporter NIPAPANTHERPTHR12570UNCHARACTERIZEDcoord: 21..361
NoneNo IPR availableGENE3D1.10.3730.20coord: 25..138
e-value: 1.1E-6
score: 30.9
NoneNo IPR availablePANTHERPTHR12570:SF65MAGNESIUM TRANSPORTER NIPA9-RELATEDcoord: 21..361
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 45..139
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 210..307

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy7G130840.1Chy7G130840.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0015693 magnesium ion transport
cellular_component GO:0005769 early endosome
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity