Chy7G129720 (gene) Cucumber (hystrix) v1

Overview
NameChy7G129720
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionheat stress transcription factor B-4
LocationchrH07: 1211443 .. 1212546 (-)
RNA-Seq ExpressionChy7G129720
SyntenyChy7G129720
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTCTAATGCTTGATACTTGTGATGGCGTTTTGCTTTCTTTAGACTCACACAAAGCAATCCCAGCTCCCTTTTTAACCAAAACTTACCAACTTGTCGATGATCCTTCCACTGACCATATTGTTTCATGGGGTGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTCCTCCCTAACTATTTCAAACACAACAATTTCTCTAGTTTTGTACGCCAGCTCAACACTTATGTATGTTCTCTTCATCATCATCGTCATCATCGTTTTTTTTTTTTTTTTTATAAAACTTTCAATCCCCATTTATTTATTTAACTTGAATTTCTCTTTTTTATTTAGGGTTTTAGAAAAATTGTACCTGATAGATGGGAGTTTGCGAACGAGTTCTTTAGAAAAGGGGAGAAGCATTTATTATGTGAGATCCATAGACGGAAAACTGCTCAACCACAAGTTACCGTTAACCAACACCATCAATCTCATTCTCCACTTAATCCCGGTTTCTACCATTTCCCCACTGCTCGACTTAGTATTTCCCCTTCCGATTCCGACGACCAAAACAATTACTGGTGTGACTCGCCGTCGCCCAACAATAATAATAACAACAACTCCGTTACCGCTCTGTCGGAAGACAATGAACGACTCCGTCGGAGTAACAATATGCTGATCTCGGAGTTAGCCCATATGAAAAAACTCTACAACGATATCATTTATTTCGTTCAAAACCACGTGAAGCCGGTTGCTCCCAGTAATTCATATCAATACTCAACCACCACGTCGTTACTCTCCGATGGGTTTCCGGTTGTACGACAACCCAACCATTACCATCATCATCATCATCATCAACAAGTATCGAGTCAGATCCGGAATAATACCGTTGGAACGAAAAGCTTCGTGACGATTCTAGAAGAAGAACAGCAACAACAACAAACGAAAACGAAGCTTTTCGGTGTGGCGATTCAATCCAAGAAACGGTTACATCCAGAGTATGGTAATTCTAATAGCAGCAACAACAACAACAACAATAAAGCGAGATTAGTTTTGGAAAAAGACGATTTAGGCCTTAATCTCATGCCTCCTTCTGCTTGTTAA

mRNA sequence

ATGGCTCTAATGCTTGATACTTGTGATGGCGTTTTGCTTTCTTTAGACTCACACAAAGCAATCCCAGCTCCCTTTTTAACCAAAACTTACCAACTTGTCGATGATCCTTCCACTGACCATATTGTTTCATGGGGTGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTCCTCCCTAACTATTTCAAACACAACAATTTCTCTAGTTTTGTACGCCAGCTCAACACTTATGGTTTTAGAAAAATTGTACCTGATAGATGGGAGTTTGCGAACGAGTTCTTTAGAAAAGGGGAGAAGCATTTATTATGTGAGATCCATAGACGGAAAACTGCTCAACCACAAGTTACCGTTAACCAACACCATCAATCTCATTCTCCACTTAATCCCGGTTTCTACCATTTCCCCACTGCTCGACTTAGTATTTCCCCTTCCGATTCCGACGACCAAAACAATTACTGGTGTGACTCGCCGTCGCCCAACAATAATAATAACAACAACTCCGTTACCGCTCTGTCGGAAGACAATGAACGACTCCGTCGGAGTAACAATATGCTGATCTCGGAGTTAGCCCATATGAAAAAACTCTACAACGATATCATTTATTTCGTTCAAAACCACGTGAAGCCGGTTGCTCCCAGTAATTCATATCAATACTCAACCACCACGTCGTTACTCTCCGATGGGTTTCCGGTTGTACGACAACCCAACCATTACCATCATCATCATCATCATCAACAAGTATCGAGTCAGATCCGGAATAATACCGTTGGAACGAAAAGCTTCGTGACGATTCTAGAAGAAGAACAGCAACAACAACAAACGAAAACGAAGCTTTTCGGTGTGGCGATTCAATCCAAGAAACGGTTACATCCAGAGTATGGTAATTCTAATAGCAGCAACAACAACAACAACAATAAAGCGAGATTAGTTTTGGAAAAAGACGATTTAGGCCTTAATCTCATGCCTCCTTCTGCTTGTTAA

Coding sequence (CDS)

ATGGCTCTAATGCTTGATACTTGTGATGGCGTTTTGCTTTCTTTAGACTCACACAAAGCAATCCCAGCTCCCTTTTTAACCAAAACTTACCAACTTGTCGATGATCCTTCCACTGACCATATTGTTTCATGGGGTGAAGATGACACTACCTTCGTCGTTTGGCGTCCTCCTGAATTCGCTAGAGATCTCCTCCCTAACTATTTCAAACACAACAATTTCTCTAGTTTTGTACGCCAGCTCAACACTTATGGTTTTAGAAAAATTGTACCTGATAGATGGGAGTTTGCGAACGAGTTCTTTAGAAAAGGGGAGAAGCATTTATTATGTGAGATCCATAGACGGAAAACTGCTCAACCACAAGTTACCGTTAACCAACACCATCAATCTCATTCTCCACTTAATCCCGGTTTCTACCATTTCCCCACTGCTCGACTTAGTATTTCCCCTTCCGATTCCGACGACCAAAACAATTACTGGTGTGACTCGCCGTCGCCCAACAATAATAATAACAACAACTCCGTTACCGCTCTGTCGGAAGACAATGAACGACTCCGTCGGAGTAACAATATGCTGATCTCGGAGTTAGCCCATATGAAAAAACTCTACAACGATATCATTTATTTCGTTCAAAACCACGTGAAGCCGGTTGCTCCCAGTAATTCATATCAATACTCAACCACCACGTCGTTACTCTCCGATGGGTTTCCGGTTGTACGACAACCCAACCATTACCATCATCATCATCATCATCAACAAGTATCGAGTCAGATCCGGAATAATACCGTTGGAACGAAAAGCTTCGTGACGATTCTAGAAGAAGAACAGCAACAACAACAAACGAAAACGAAGCTTTTCGGTGTGGCGATTCAATCCAAGAAACGGTTACATCCAGAGTATGGTAATTCTAATAGCAGCAACAACAACAACAACAATAAAGCGAGATTAGTTTTGGAAAAAGACGATTTAGGCCTTAATCTCATGCCTCCTTCTGCTTGTTAA

Protein sequence

MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQSHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQPNHYHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQQTKTKLFGVAIQSKKRLHPEYGNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC*
Homology
BLAST of Chy7G129720 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 1.5e-85
Identity = 183/334 (54.79%), Postives = 226/334 (67.66%), Query Frame = 0

Query: 19  KAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 78
           KA+PAPFLTKTYQLVDDP+TDH+VSWG+DDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 79  QLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQSHSPLNP--- 138
           QLNTYGFRKIVPDRWEFANEFF++GEKHLLCEIHRRKT+Q     +    SH    P   
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMSHHHAPPQIP 148

Query: 139 ----GFYHFPTARLSISPSDSDDQNNYWCDS--PS-----PNNNNNNNSVTALSEDNERL 198
                F+  P  R++     + ++++YWCD   PS     P   +    VTALSEDNERL
Sbjct: 149 FSGGSFFPLPPPRVT-----TPEEDHYWCDDSPPSRPRVIPQQIDTAAQVTALSEDNERL 208

Query: 199 RRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQP--- 258
           RRSN +L+SELAHMKKLYNDIIYFVQNHVKPVAPSN+  Y   +S L       +QP   
Sbjct: 209 RRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAPSNNSSY--LSSFLQK--QQQQQPPTL 268

Query: 259 NHYHHHHHHQQVSSQIRNNTVGTKSFVTILEEE-----QQQQQTKTKLFGVAIQSKKRLH 318
           ++Y+    +    + + ++   ++S +T+LE++      Q    KTKLFGV++ S K+  
Sbjct: 269 DYYNTATVNATNLNALNSSPPTSQSSITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRS 328

Query: 319 PEYGNSNSSNNNNNNKARLVLEKDDLGLNLMPPS 331
             + +  S       K RLVL++ DL LNLM  S
Sbjct: 329 HHFSDQTS-------KTRLVLDQSDLALNLMTAS 346

BLAST of Chy7G129720 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.2e-68
Identity = 139/248 (56.05%), Postives = 167/248 (67.34%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLD----------SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTT 60
           MA +++ C  +++S++          + K +PAPFLTKTYQLVDDP TDHIVSWGEDDTT
Sbjct: 1   MAFLVERCGEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAE 120

Query: 121 IHRRKTAQPQVTVNQH--HQSHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNN 180
           IHRRK++QP      H  +  H  LNP     P           ++       + + +  
Sbjct: 121 IHRRKSSQPPPPPMPHQPYHHHHHLNPFSLPPPPPAYHHHHLIQEEPATTAHCTVAGDGG 180

Query: 181 NNNNSVTALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTT 237
              + + ALSEDN +LRR N++L+SELAHMKKLYNDIIYF+QNHV PV  + +   ST  
Sbjct: 181 EGGDFLAALSEDNRQLRRRNSLLLSELAHMKKLYNDIIYFLQNHVAPVTTTTTTPSSTAM 240

BLAST of Chy7G129720 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 2.0e-66
Identity = 140/237 (59.07%), Postives = 169/237 (71.31%), Query Frame = 0

Query: 1   MALMLDTCDG-VLLSLD-SH----------KAIPAPFLTKTYQLVDDPSTDHIVSWGEDD 60
           MA +++ C G +++S++ SH           A PAPFL+KTYQLVDDPSTD +VSWGED+
Sbjct: 1   MAFLVERCGGEMVVSMERSHGRSTTTAAAVTAAPAPFLSKTYQLVDDPSTDDVVSWGEDE 60

Query: 61  TTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLL 120
            TFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL
Sbjct: 61  ATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLL 120

Query: 121 CEIHRRKT---AQPQVTVNQHHQSHSPLNPGFYHFPTARLS----ISPSDSDDQNNYWCD 180
            EIHRRK+   +QPQ         H PL+   +  PT   S     + + +      +C 
Sbjct: 121 SEIHRRKSSSCSQPQPPPPFPMHQHYPLS--LFSPPTTPRSPPVGAAAAAAYHFQEEYCS 180

Query: 181 SPSPNNNNNNNSVTALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAP 219
           SP+       + + ALSEDN +LRR N++L+SELAHM+KLYNDIIYF+QNHV+PVAP
Sbjct: 181 SPADYAGGGGDLLAALSEDNRQLRRRNSLLLSELAHMRKLYNDIIYFLQNHVEPVAP 235

BLAST of Chy7G129720 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.9e-65
Identity = 172/390 (44.10%), Postives = 208/390 (53.33%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDD-TTFVVWRPPEFARDLLPNYFKHNNFSS 76
           + KA+PAPFLTKTYQLVDDP+TDHIVSWG+D  +TFVVWRPPEFARD+LPNYFKHNNFSS
Sbjct: 17  AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILPNYFKHNNFSS 76

Query: 77  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQSHSPLNP 136
           FVRQLNTYGFRK+VP+RWEFANEFFRKGEK LL EIHRRKT+             SP  P
Sbjct: 77  FVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTSSASTA--------SPSPP 136

Query: 137 GFYHFPTARLSISPSDSDDQNN----------------------YWCD------------ 196
            F+  P   L   P  +  Q++                      +W +            
Sbjct: 137 PFFAPPHFPLFHHPGVAAAQHHHAFVGDDGVVAAHGIGMPFPQPHWREPNLPVATRLLAL 196

Query: 197 ---SPSPNNNNNNN-----SVTALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHV 256
              +PSP++          +   L E+NERLRRSN  L+ ELAHM+KLYNDIIYFVQNHV
Sbjct: 197 GGPAPSPSSAEAGGAGRAATAAVLMEENERLRRSNTALLQELAHMRKLYNDIIYFVQNHV 256

Query: 257 KPVAPSNSYQYSTTTSLLSDGFPVVRQPNHYHHHHHHQQVSSQIRNNTVG---TKSFVTI 316
           +PVAPS     +    L   G    ++P            ++ + NN+ G   + S +TI
Sbjct: 257 RPVAPSP----AAAAFLQGLGMQARKKP-----------AAANVLNNSGGSTTSSSSLTI 316

Query: 317 LEE----EQQQ------------------QQTKTKLFGVAIQ-------SKKRLHPEYGN 332
            EE     QQQ                      TKLFGV +        SK+   PE   
Sbjct: 317 AEEPSPPPQQQHLAGEKSGGEAGNSSAARSSAPTKLFGVHLSAAPCGAGSKRASSPEEHP 376

BLAST of Chy7G129720 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 6.5e-57
Identity = 125/235 (53.19%), Postives = 151/235 (64.26%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDD-----TTFVVWRPPEFARDLLPNYFKHN 76
           S   +PAPFLTKTYQLVDDP+TDH+VSW +DD     ++FVVWRPPEFARD+LPNYFKH+
Sbjct: 18  SASVVPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHS 77

Query: 77  NFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA----------QPQV 136
           NFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LLCEIHRRK+A           P  
Sbjct: 78  NFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAAATWPPFPPPPPPF 137

Query: 137 TVNQHHQSHSPLNPG-----------------FYHFPTARLSISPSDSDDQNNYWCDSPS 196
              +H  + +    G                  + F +A L ++PS           +P+
Sbjct: 138 FAPRHFAAGAFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPSSRLLSQLGPVIAPA 197

Query: 197 PNNNNNNNSVTALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPS 220
                      AL ++N RL R N  L+ ELAHM+KLY+DIIYFVQNHV+PVAPS
Sbjct: 198 -RRAAATPEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHVRPVAPS 251

BLAST of Chy7G129720 vs. ExPASy TrEMBL
Match: A0A0A0L4W8 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 PE=3 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.1e-183
Identity = 326/333 (97.90%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180
           VTVNQHHQ HSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSP NNNNNNSVTALSED
Sbjct: 121 VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSP-NNNNNNSVTALSED 180

Query: 181 NERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240
           NERLRRSNNML+SELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ
Sbjct: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240

Query: 241 PNHY-HHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQQTKTKLFGVAIQSKKRLHPEY 300
           PNHY HHHHHHQQVSSQIRNNTVGTKSFVTILEEE QQQQTKTKLFGVAIQSKKRLHPEY
Sbjct: 241 PNHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEE-QQQQTKTKLFGVAIQSKKRLHPEY 300

Query: 301 GNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 333
           GNSNS  NNNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 GNSNS--NNNNNKARLVLEKDDLGLNLMPPSAC 329

BLAST of Chy7G129720 vs. ExPASy TrEMBL
Match: A0A5A7VAY1 (Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003260 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.4e-179
Identity = 320/333 (96.10%), Postives = 324/333 (97.30%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD+CDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDSCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180
           VTVNQHHQS SPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED
Sbjct: 121 VTVNQHHQSLSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180

Query: 181 NERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240
           NERLRRSNNML+SELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST+TSLLSDGFPVVRQ
Sbjct: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSTSLLSDGFPVVRQ 240

Query: 241 PNHYHHHHHHQQVSSQIRNN-TVGTKSFVTILEEEQQQQQTKTKLFGVAIQSKKRLHPEY 300
           PNHYHHHH   QVSSQIRNN TVGTKSFVTILEEEQQQQQ KTKLFGVAIQSKKRLHPEY
Sbjct: 241 PNHYHHHH---QVSSQIRNNTTVGTKSFVTILEEEQQQQQPKTKLFGVAIQSKKRLHPEY 300

Query: 301 GNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 333
           GNS+S   NNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 GNSHS---NNNNKARLVLEKDDLGLNLMPPSAC 327

BLAST of Chy7G129720 vs. ExPASy TrEMBL
Match: A0A1S3BUY5 (heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103493553 PE=3 SV=1)

HSP 1 Score: 637.9 bits (1644), Expect = 2.4e-179
Identity = 320/333 (96.10%), Postives = 324/333 (97.30%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD+CDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDSCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180
           VTVNQHHQS SPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED
Sbjct: 121 VTVNQHHQSLSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180

Query: 181 NERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240
           NERLRRSNNML+SELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST+TSLLSDGFPVVRQ
Sbjct: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSTSLLSDGFPVVRQ 240

Query: 241 PNHYHHHHHHQQVSSQIRNN-TVGTKSFVTILEEEQQQQQTKTKLFGVAIQSKKRLHPEY 300
           PNHYHHHH   QVSSQIRNN TVGTKSFVTILEEEQQQQQ KTKLFGVAIQSKKRLHPEY
Sbjct: 241 PNHYHHHH---QVSSQIRNNTTVGTKSFVTILEEEQQQQQPKTKLFGVAIQSKKRLHPEY 300

Query: 301 GNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 333
           GNS+S   NNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 GNSHS---NNNNKARLVLEKDDLGLNLMPPSAC 327

BLAST of Chy7G129720 vs. ExPASy TrEMBL
Match: A0A6J1IW73 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111481146 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.5e-144
Identity = 283/352 (80.40%), Postives = 301/352 (85.51%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSP--LNPGFYHFPTARLSISPSDSDDQNNYWCDSP--SPNNNNN---NNS 180
           VTVNQHHQS+SP  LN GFYH+P AR+SISPSDSDD  N WCDSP  S NNNNN   NNS
Sbjct: 121 VTVNQHHQSYSPLGLNAGFYHYP-ARVSISPSDSDDNPN-WCDSPPLSSNNNNNNVFNNS 180

Query: 181 VTALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSD 240
           VTALSEDNERLRRSNNML+SELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS  TSLLSD
Sbjct: 181 VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS--TSLLSD 240

Query: 241 GFPV-VRQPNHY---HHHHHHQQVSSQI---------RNNTVGTKSFVTILEEEQQQQQT 300
           GFPV  RQPNH+   +H  + +QVS Q           NN   +KSFVTI+EE       
Sbjct: 241 GFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVGGTPNNNNNNGSSKSFVTIVEE------P 300

Query: 301 KTKLFGVAIQSKKRLHPEYGNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 333
           KTKLFGV++QSKKR+HPEYG++N +    NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 KTKLFGVSLQSKKRVHPEYGSNNIA--KENNKARLVLEKDDLGLNLMPPSAC 340

BLAST of Chy7G129720 vs. ExPASy TrEMBL
Match: A0A6J1FJH2 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111445920 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 2.5e-144
Identity = 283/349 (81.09%), Postives = 300/349 (85.96%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSP--LNPGFYHFPTARLSISPSDSDDQNNYWCDSP--SPNNNNN--NNSV 180
           VTVNQHHQS+SP  LN GFYH+P AR+SISPSDSDD  N WCDSP  S NNNNN  NNSV
Sbjct: 121 VTVNQHHQSYSPLGLNAGFYHYP-ARVSISPSDSDDNPN-WCDSPPLSSNNNNNNFNNSV 180

Query: 181 TALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDG 240
           TALSEDNERLRRSNNML+SELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS  TSLLSDG
Sbjct: 181 TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS--TSLLSDG 240

Query: 241 FPV-VRQPNHY---HHHHHHQQVSSQI-------RNNTVGTKSFVTILEEEQQQQQTKTK 300
           FPV  RQPNH+   +H  + +QVS Q         NN   +KSFVTI+EE       KTK
Sbjct: 241 FPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPNNNNGSSKSFVTIVEE------PKTK 300

Query: 301 LFGVAIQSKKRLHPEYGNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 333
           LFGV++QSKKR+HPEYG++N      NNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 LFGVSLQSKKRVHPEYGSNNIV--KENNKARLVLEKDDLGLNLMPPSAC 337

BLAST of Chy7G129720 vs. NCBI nr
Match: XP_004141262.2 (heat stress transcription factor B-4 [Cucumis sativus] >KAE8649863.1 hypothetical protein Csa_012628 [Cucumis sativus])

HSP 1 Score: 649 bits (1675), Expect = 7.46e-235
Identity = 325/333 (97.60%), Postives = 327/333 (98.20%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180
           VTVNQHHQ HSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNN SVTALSED
Sbjct: 121 VTVNQHHQPHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNN-SVTALSED 180

Query: 181 NERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240
           NERLRRSNNML+SELAH+KKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ
Sbjct: 181 NERLRRSNNMLMSELAHIKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240

Query: 241 PNHYHHHHHH-QQVSSQIRNNTVGTKSFVTILEEEQQQQQTKTKLFGVAIQSKKRLHPEY 300
           PNHYHHHHHH QQVSSQIRNNTVGTKSFVTILEEEQQQQ TKTKLFGVAIQSKKRLHPEY
Sbjct: 241 PNHYHHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQ-TKTKLFGVAIQSKKRLHPEY 300

Query: 301 GNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 332
           GNSNS  NNNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 GNSNS--NNNNNKARLVLEKDDLGLNLMPPSAC 329

BLAST of Chy7G129720 vs. NCBI nr
Match: XP_008452568.1 (PREDICTED: heat stress transcription factor B-4 [Cucumis melo] >KAA0064394.1 heat stress transcription factor B-4 [Cucumis melo var. makuwa] >TYK20192.1 heat stress transcription factor B-4 [Cucumis melo var. makuwa])

HSP 1 Score: 639 bits (1647), Expect = 1.28e-230
Identity = 320/333 (96.10%), Postives = 324/333 (97.30%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD+CDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDSCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180
           VTVNQHHQS SPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED
Sbjct: 121 VTVNQHHQSLSPLNPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTALSED 180

Query: 181 NERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQ 240
           NERLRRSNNML+SELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST+TSLLSDGFPVVRQ
Sbjct: 181 NERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSTSLLSDGFPVVRQ 240

Query: 241 PNHYHHHHHHQQVSSQIRNNT-VGTKSFVTILEEEQQQQQTKTKLFGVAIQSKKRLHPEY 300
           PNHYHHHH   QVSSQIRNNT VGTKSFVTILEEEQQQQQ KTKLFGVAIQSKKRLHPEY
Sbjct: 241 PNHYHHHH---QVSSQIRNNTTVGTKSFVTILEEEQQQQQPKTKLFGVAIQSKKRLHPEY 300

Query: 301 GNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 332
           GNS+S   NNNNKARLVLEKDDLGLNLMPPSAC
Sbjct: 301 GNSHS---NNNNKARLVLEKDDLGLNLMPPSAC 327

BLAST of Chy7G129720 vs. NCBI nr
Match: XP_038897232.1 (heat stress transcription factor B-4-like [Benincasa hispida])

HSP 1 Score: 558 bits (1439), Expect = 1.65e-198
Identity = 295/360 (81.94%), Postives = 307/360 (85.28%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPL---NPGFYHFPTARLSISPSDSDDQNNYWCDSPSPNN----NNNNNS 180
           VTVNQHHQSHSPL   NPGFYHFP  R+SISPSDSDDQNN WCDSPS ++    NNNNNS
Sbjct: 121 VTVNQHHQSHSPLGINNPGFYHFP-GRVSISPSDSDDQNN-WCDSPSLSSPAPHNNNNNS 180

Query: 181 VTALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSD 240
           VTALSEDNERLRRSNNML+SELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTT SLLSD
Sbjct: 181 VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTT-SLLSD 240

Query: 241 GFPVVRQ--PNHYHHHHH-------------------HQQVSSQIRNNTVGTKSFVTILE 300
            FPVV+Q  PNH+HHH+H                   H   ++ I NN  GTKSFVTILE
Sbjct: 241 RFPVVQQRPPNHHHHHYHQQSQIHLVTGTTPTNNNNNHNNNNNIIINNNSGTKSFVTILE 300

Query: 301 EEQQQQQTKTKLFGVAIQSKKRLHPEYGNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 332
           E    Q TKTKLFGVAIQSKKRLHPEYG++N    NNNNKARLVLE DDLGLNLMPPS C
Sbjct: 301 E----QPTKTKLFGVAIQSKKRLHPEYGSNNIGKENNNNKARLVLENDDLGLNLMPPSTC 353

BLAST of Chy7G129720 vs. NCBI nr
Match: XP_022940239.1 (heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 523 bits (1347), Expect = 9.40e-185
Identity = 283/349 (81.09%), Postives = 300/349 (85.96%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPL--NPGFYHFPTARLSISPSDSDDQNNYWCDSP--SPNNNNNN--NSV 180
           VTVNQHHQS+SPL  N GFYH+P AR+SISPSDSDD  N WCDSP  S NNNNNN  NSV
Sbjct: 121 VTVNQHHQSYSPLGLNAGFYHYP-ARVSISPSDSDDNPN-WCDSPPLSSNNNNNNFNNSV 180

Query: 181 TALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDG 240
           TALSEDNERLRRSNNML+SELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST  SLLSDG
Sbjct: 181 TALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST--SLLSDG 240

Query: 241 FPV-VRQPNHY---HHHHHHQQVSSQIR-------NNTVGTKSFVTILEEEQQQQQTKTK 300
           FPV  RQPNH+   +H  + +QVS Q         NN   +KSFVTI+EE       KTK
Sbjct: 241 FPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPNNNNGSSKSFVTIVEEP------KTK 300

Query: 301 LFGVAIQSKKRLHPEYGNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 332
           LFGV++QSKKR+HPEYG++N    NN  KARLVLEKDDLGLNLMPPSAC
Sbjct: 301 LFGVSLQSKKRVHPEYGSNNIVKENN--KARLVLEKDDLGLNLMPPSAC 337

BLAST of Chy7G129720 vs. NCBI nr
Match: XP_022982267.1 (heat stress transcription factor B-4-like [Cucurbita maxima])

HSP 1 Score: 523 bits (1347), Expect = 1.05e-184
Identity = 283/352 (80.40%), Postives = 301/352 (85.51%), Query Frame = 0

Query: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 VTVNQHHQSHSPL--NPGFYHFPTARLSISPSDSDDQNNYWCDSP--SPNNNNNN---NS 180
           VTVNQHHQS+SPL  N GFYH+P AR+SISPSDSDD  N WCDSP  S NNNNNN   NS
Sbjct: 121 VTVNQHHQSYSPLGLNAGFYHYP-ARVSISPSDSDDNPN-WCDSPPLSSNNNNNNVFNNS 180

Query: 181 VTALSEDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSD 240
           VTALSEDNERLRRSNNML+SELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST  SLLSD
Sbjct: 181 VTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST--SLLSD 240

Query: 241 GFPV-VRQPNHY---HHHHHHQQVSSQIR---------NNTVGTKSFVTILEEEQQQQQT 300
           GFPV  RQPNH+   +H  + +QVS Q           NN   +KSFVTI+EE       
Sbjct: 241 GFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVGGTPNNNNNNGSSKSFVTIVEEP------ 300

Query: 301 KTKLFGVAIQSKKRLHPEYGNSNSSNNNNNNKARLVLEKDDLGLNLMPPSAC 332
           KTKLFGV++QSKKR+HPEYG++N +  NN  KARLVLEKDDLGLNLMPPSAC
Sbjct: 301 KTKLFGVSLQSKKRVHPEYGSNNIAKENN--KARLVLEKDDLGLNLMPPSAC 340

BLAST of Chy7G129720 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 317.8 bits (813), Expect = 1.1e-86
Identity = 183/334 (54.79%), Postives = 226/334 (67.66%), Query Frame = 0

Query: 19  KAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 78
           KA+PAPFLTKTYQLVDDP+TDH+VSWG+DDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR
Sbjct: 29  KAVPAPFLTKTYQLVDDPATDHVVSWGDDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVR 88

Query: 79  QLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQSHSPLNP--- 138
           QLNTYGFRKIVPDRWEFANEFF++GEKHLLCEIHRRKT+Q     +    SH    P   
Sbjct: 89  QLNTYGFRKIVPDRWEFANEFFKRGEKHLLCEIHRRKTSQMIPQQHSPFMSHHHAPPQIP 148

Query: 139 ----GFYHFPTARLSISPSDSDDQNNYWCDS--PS-----PNNNNNNNSVTALSEDNERL 198
                F+  P  R++     + ++++YWCD   PS     P   +    VTALSEDNERL
Sbjct: 149 FSGGSFFPLPPPRVT-----TPEEDHYWCDDSPPSRPRVIPQQIDTAAQVTALSEDNERL 208

Query: 199 RRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVRQP--- 258
           RRSN +L+SELAHMKKLYNDIIYFVQNHVKPVAPSN+  Y   +S L       +QP   
Sbjct: 209 RRSNTVLMSELAHMKKLYNDIIYFVQNHVKPVAPSNNSSY--LSSFLQK--QQQQQPPTL 268

Query: 259 NHYHHHHHHQQVSSQIRNNTVGTKSFVTILEEE-----QQQQQTKTKLFGVAIQSKKRLH 318
           ++Y+    +    + + ++   ++S +T+LE++      Q    KTKLFGV++ S K+  
Sbjct: 269 DYYNTATVNATNLNALNSSPPTSQSSITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKKRS 328

Query: 319 PEYGNSNSSNNNNNNKARLVLEKDDLGLNLMPPS 331
             + +  S       K RLVL++ DL LNLM  S
Sbjct: 329 HHFSDQTS-------KTRLVLDQSDLALNLMTAS 346

BLAST of Chy7G129720 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 181.0 bits (458), Expect = 1.5e-45
Identity = 101/205 (49.27%), Postives = 130/205 (63.41%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           + +++PAPFL+KTYQLVDD STD +VSW E+ T FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRK-----TAQPQVTVNQHHQSHS 136
           +RQLNTYGFRK VPD+WEFAN++FR+G + LL +I RRK     TA   V V    +S+S
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRKSVIASTAGKCVVVGSPSESNS 126

Query: 137 PLNPGFYHFPTARLSISPSDSDDQNNYWCDSP--SPNNNNNNNSVTALSEDNERLRRSNN 196
                                DD  +    SP  S N  +  N V  LS +NE+L+R NN
Sbjct: 127 ------------------GGGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKRENN 186

Query: 197 MLISELAHMKKLYNDIIYFVQNHVK 215
            L SELA  KK  ++++ F+  H+K
Sbjct: 187 NLSSELAAAKKQRDELVTFLTGHLK 193

BLAST of Chy7G129720 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 178.7 bits (452), Expect = 7.7e-45
Identity = 102/237 (43.04%), Postives = 138/237 (58.23%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           S ++IP PFLTKT+ LV+D S D ++SW ED ++F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTV-----NQHHQSHS 136
           VRQLNTYGF+K+VPDRWEF+N+FF++GEK LL EI RRK      TV      Q +Q+  
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITTTHQTVVAPSSEQRNQT-- 135

Query: 137 PLNPGFYHFPTARLSISPSDS-DDQNNYWCDSPSPNN--------NNNNNSVTALSEDNE 196
                        + +SPS+S +D NN    S SP++          N      L E+NE
Sbjct: 136 -------------MVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLEENE 195

Query: 197 RLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVVR 240
           +LR  N  L  EL  MK + ++I   + N+V       SY    ++S   +  P  R
Sbjct: 196 KLRSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSYSPGGSSSQPMEFLPAKR 237

BLAST of Chy7G129720 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.4e-43
Identity = 119/316 (37.66%), Postives = 164/316 (51.90%), Query Frame = 0

Query: 16  DSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSS 75
           DS ++IP PFLTKTYQLV+DP  D ++SW ED TTF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 76  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQSHSPLNP 135
           FVRQLNTYGFRK+VPDRWEF+N+ F++GEK LL +I RRK +QP +       + +    
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAAS 170

Query: 136 G--FYHFPTARLSISPSDSDDQNNYWCDSP---------------SPNNNNNNNSVTALS 195
                  P     +SPS+S ++     +S                S     +  +   L 
Sbjct: 171 AVTVAAVPVVAHIVSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCTTAPELV 230

Query: 196 EDNERLRRSNNMLISELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSLLSDGFPVV 255
           E+NERLR+ N  L  E+  +K LY +I   + N                  LL +G P+ 
Sbjct: 231 EENERLRKDNERLRKEMTKLKGLYANIYTLMANFTP--------GQEDCAHLLPEGKPLD 290

Query: 256 RQPNHYHHHHHHQQVSSQIRNNTVGTKSFVTILEEEQQQQQTKTKLFGVAIQSKK-RLHP 314
             P         Q++S  I  + + T   + + E+         +LFGV+I  K+ R   
Sbjct: 291 LLP-------ERQEMSEAIMASEIETGIGLKLGED------LTPRLFGVSIGVKRARREE 345

BLAST of Chy7G129720 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 156.8 bits (395), Expect = 3.1e-38
Identity = 89/187 (47.59%), Postives = 111/187 (59.36%), Query Frame = 0

Query: 22  PAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLN 81
           P PFL+KTY +VDD +TD IVSW  ++ +F+VW+PPEFARDLLP  FKHNNFSSFVRQLN
Sbjct: 35  PPPFLSKTYDMVDDHNTDSIVSWSANNNSFIVWKPPEFARDLLPKNFKHNNFSSFVRQLN 94

Query: 82  TYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQVTVNQHHQSHSPLNPGFYHFP 141
           TYGFRK+ PDRWEFANE F +G+KHLL  I RRK A  Q    Q HQ             
Sbjct: 95  TYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQ---GQGHQR------------ 154

Query: 142 TARLSISPSDSDDQNNYWCDSPSPNNNNNNNSVTA--------LSEDNERLRRSNNMLIS 201
                                 S ++N  N+SV+A        L E+ ERL+R  N+L+ 
Sbjct: 155 ----------------------SQHSNGQNSSVSACVEVGKFGLEEEVERLKRDKNVLMQ 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6351.5e-8554.79Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q7XHZ02.2e-6856.05Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q10KX82.0e-6659.07Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q67U942.9e-6544.10Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R86.5e-5753.19Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
A0A0A0L4W86.1e-18397.90HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 ... [more]
A0A5A7VAY12.4e-17996.10Heat stress transcription factor B-4 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S3BUY52.4e-17996.10heat stress transcription factor B-4 OS=Cucumis melo OX=3656 GN=LOC103493553 PE=... [more]
A0A6J1IW732.5e-14480.40heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FJH22.5e-14481.09heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_004141262.27.46e-23597.60heat stress transcription factor B-4 [Cucumis sativus] >KAE8649863.1 hypothetica... [more]
XP_008452568.11.28e-23096.10PREDICTED: heat stress transcription factor B-4 [Cucumis melo] >KAA0064394.1 hea... [more]
XP_038897232.11.65e-19881.94heat stress transcription factor B-4-like [Benincasa hispida][more]
XP_022940239.19.40e-18581.09heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 h... [more]
XP_022982267.11.05e-18480.40heat stress transcription factor B-4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G46264.11.1e-8654.79heat shock transcription factor B4 [more]
AT4G36990.11.5e-4549.27heat shock factor 4 [more]
AT5G62020.17.7e-4543.04heat shock transcription factor B2A [more]
AT4G11660.11.4e-4337.66winged-helix DNA-binding transcription factor family protein [more]
AT1G32330.13.1e-3847.59heat shock transcription factor A1D [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 76..88
score: 84.62
coord: 25..48
score: 47.38
coord: 63..75
score: 78.68
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 5.1E-60
score: 215.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 2.5E-32
score: 111.4
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..116
e-value: 1.2E-37
score: 130.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 149..178
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 12..330
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 12..330
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy7G129720.1Chy7G129720.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding