Chy7G129690 (gene) Cucumber (hystrix) v1

Overview
NameChy7G129690
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionLipase_3 domain-containing protein
LocationchrH07: 1171052 .. 1174788 (-)
RNA-Seq ExpressionChy7G129690
SyntenyChy7G129690
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTCTAACGAGATACAATTGAATGAAAAAGGTCTGATATTAAAGCCAGATAAGGCAACAGTAAGAGATGTATTTGAGTTCTTACTTCCTTCGGGTTCCAGAAAGATAAAAAAGTTGATGGAATGTCCAGACGACCAAATTGGGCTTTACACCAATTTCAAAGCTCGATGGATCATTTCTGTTTCAATTTTGACCCAGAAATTCCTTTTTGCCATCGCTTCTCTTTTCACAATCTTAGTTGCATTTTGGTCAATAATTCAACAGTTTTTCTACAAGTATGTGCTGGCAGCAGAATTTTGGGTTGCGCCATCTCGGATTATTAATTTCACGGATGTGCAGCCCAGAGTGAGACGTAGTGACTGGGAATTGCTGGTTCCCGACGACAATGTAGACATGAATTCACAAGACCGTGATTTCAAATATTATAGTGCCTTAACCATTATGGCTTCCAAATTGGCATATCAAGATTATTCACATTCCTCCTCCCTCGTTGAGTTCGTAGTAAATGACTGCTGGCAGGTACTCCAAAATTAATTTTAATTTTAACTTTAAGTAGTTGTTTTAAGTGTTGGGTTGAGTTATTTATAAGTTAGAAATTTTGTTTTAGTTTAAGAAAAAGTTATAGAATTTATATGTTTATCTTGAATTAACCCAATTATAGTAAGTTAGTTTTTTTTAATTTTATTTTACTGTGTGGTGCTTGTGGGTTAGACACTGCCTTGATGGAAGAAAAAGTTATATATTCTCATCTGGTTTTCTTGTTTAAATAATTATATATAAACACTTTAATTGATCGAGTACGAAAAAACTAAGGTCTCGTTTATTAATCCATTTTGGTTTTTGTTTTTTTTTTTAAATTTAGGTATATTTTCTCTCTATATCTTTATCTTGATTTGTATCTAATTCCTTAATTATAATAATTGAGTTTTTAGCTAAATTTTAAAAACAAAAAGAAGTAGAAAGTGATTATGAAATAGAAGTTAAGTAATTTTAAAGAAAAACAGCCAACCCTAATGTTAAATTCTGATTGGAATATATAAACTGATGAGTTTTCTTCTTCCCTTTCTGTTTCATTTGTGAATAATATTCATATGCAAGTAAACTTTGCAATATTTATAAACTTTGGGTAATTGGTTATTGGTTATTAGTTTTTCAGAGAACAAAAATTATTTTATAGCTAATTTTGATAAACACGTACAGATATGTATTAGAATTTCATATGTATTGTTCATTTTATGTTAAGTCTTGTAGTTTTTCTATTTAATCTCATCTCATTTGTAATATTTACAAGAACATTGAATGCAATATTCAAATCTTCTCTCAAACTTGATACAAATCCATGATGTTATTTTTTGGTTTTCGAATGCAGATGAAATTAATTGATTGTCGCAATTTCTGGAACGGTAAGATTTTTTTTTGTTGAACTTCTAATTATCAAATTAAATATAAATAAGGACTTTGGAATTCTCTTTTTCAAATAAATAAAGACTCTGAAATGAACTTCTACTTTTCTAACTAAATAAAGACGGTTGACTTCCATATTTTTTTGAATAAATAAAGACTTAGCTTGGTCAACTTTTTTTAGTTGTATTATAAAAAAAATGTATTTATATTATCAATCACAATAATATTAAAACTTAGGTGCACCTTGTTTAATTTACTATATTTTAGAGTTTAGGATTTGGGAAGATATTAAAGTTCTTACAAGCCTTTGGGCAAGCAGATCCTCCCCTTTGTTCTCAAACTATACAGCCAACACTATAGTTCTTAATATCAATATTTTAATTTGTTCAATTGTACTGATTATGGTTTTTCTTGGATTTTCCTCTAGCCATTGTTTTTATTTTCCTTGCTTTTTGTACTTATCGATTATCTATTAATGAAGTGGTAATGATGATGGTGCTACGGAGGCGTTCACCTAATGGAGATGTACTAGTGCACATACTTATTCATCTCGTATCTTTTTTTTAAGACAACTTTTTTACTTTTTTTGTCATATAAAGATTTCCATTTGTTGAATTCTTATCCAAATTCTAGAAACAAAAAGAATGTTTTTTAAAAACTACATATTTTGCAGACTTCCAAAACAAAGCTACGACTCATGCAATTATGTTTGAGAACACAGGCAAAGATCCAAACGTAACAGTGGTTGCATTCAGAGGGACATCCGTACTTGACATTAACGATTGGATGGTTGATCTGAACTTTTCTTGGTTCCTATTAGAAGGCAAGGTCGGCATCCATTCCGGGTTCATGCAAGCCCTTGGCTACCAAAAGTCCGGTGATTGGCCCAAAGAACTCACCGATCCCAAACATGAATTTGCATATTACTTCCTTCGCCAAAACCTAAGAGAAATTGCTAAATCCAATGACAATGCAAAATTCATCATCACTGGTCATAGTTTAGGTGGTGCCCTTGCTACCCTTTTTGTCACTTTGTTAGCCTACCACAATGAAACAATCCTTCTTGACAAGATTCAAGCTGTTTATACCTTTGGTCAGCCTAGAGTTGGGAATCAAAGTTTTGCGCAGCTTATGGTCAAAACTTTCAAAACTCATGACATTAAATATTATAGATATGTTTACTCTTTTGATTTGGTTCCTAGAATTCCTTTCGATTCTCTTGCTAATTTCAGCTACAGACACTTTGGTGGATGCGTCTACTTTGATGTCTTCTACAATGGCAAGGTGACTCTCTACCTGTTTGATCATTTATTCGGTTTCTTCGTGGTTCTATTAACAACACATTTTTTCAATAATATATTTGTCTCCTTCTCCATCAACTTTTGAATCAATTTAAAATGGTATCATACAACGTGATCTCTAAAGAGAGGTGGTCTTAGACCATAATGCTTTGATAATAACATCTTAAGACTTAAATCACTAATTGATGCAAAAGCTTTGGACTATACGGATGGAAAGATATTAAAGCACTTACGGGTCTTTGGACTAGTAGATCCAAACTTTTTTCAAATTATCCACCGAGTTCTATTGCTCTTAATTTAAATGCGTTTGACTAGTCTATTAGCTCTGCTTTTCTTGTTGGGTCTAAGGGCTTTTCTCTAGCCCTTTTTGTATTCTTCTTGCTTCATGTACTCATTTTTCTATATTAATGAAGCGAGAATGAAGAGGGTGTTAAGGGGTGTCTACATAGTGGAGATGTTCTGGTGCACCTGCTGACCCAATTGTATCTTTTTTAAAAAAATATATATAAAATATAAGAGAATTGTTGTGTAATTAAAATTAAATATATATTTTGGTTCATATAACAAAAGTTGTTGGCTGGGACAATAATCTATATCATTACTACACTGACATCTACATTATATTATATGTTGTGCTGGTATATCTTTGAATTTAATTTCTTTTATTTTCGATAATTAAAGAGTGAATAAGTTTTTTTTTTTTTTTTTTTTGGGTGTTTTATGTATAATCAGTTTTTGAAGGAGCAGCCAAACACAAACTACTTTTCACCAATATGGGTTATACCCAAATACTTGAGTGCAGATTGGGAGTTCATTAGAAGTTTAATAATAACCCCAATTGTGAAGGGTAGAAAGTACTTCGAGGGTTTTTTTACAATAATGGAAAGGACAGTTGGATTGGTTATTCCGGGAATATCAGCTCATGTATGTTCAAATTATGTCAACATGACTCGTTGGGGGAATATACAGTTGCCACCTGACCACCACGAACTCTTGAAGTTTGCTCACTACATTGAAGCTGACTACTAG

mRNA sequence

ATGGGTTCTAACGAGATACAATTGAATGAAAAAGGTCTGATATTAAAGCCAGATAAGGCAACAGTAAGAGATGTATTTGAGTTCTTACTTCCTTCGGGTTCCAGAAAGATAAAAAAGTTGATGGAATGTCCAGACGACCAAATTGGGCTTTACACCAATTTCAAAGCTCGATGGATCATTTCTGTTTCAATTTTGACCCAGAAATTCCTTTTTGCCATCGCTTCTCTTTTCACAATCTTAGTTGCATTTTGGTCAATAATTCAACAGTTTTTCTACAAGTATGTGCTGGCAGCAGAATTTTGGGTTGCGCCATCTCGGATTATTAATTTCACGGATGTGCAGCCCAGAGTGAGACGTAGTGACTGGGAATTGCTGGTTCCCGACGACAATGTAGACATGAATTCACAAGACCGTGATTTCAAATATTATAGTGCCTTAACCATTATGGCTTCCAAATTGGCATATCAAGATTATTCACATTCCTCCTCCCTCGTTGAGTTCGTAGTAAATGACTGCTGGCAGATGAAATTAATTGATTGTCGCAATTTCTGGAACGACTTCCAAAACAAAGCTACGACTCATGCAATTATGTTTGAGAACACAGGCAAAGATCCAAACGTAACAGTGGTTGCATTCAGAGGGACATCCGTACTTGACATTAACGATTGGATGGTTGATCTGAACTTTTCTTGGTTCCTATTAGAAGGCAAGGTCGGCATCCATTCCGGGTTCATGCAAGCCCTTGGCTACCAAAAGTCCGGTGATTGGCCCAAAGAACTCACCGATCCCAAACATGAATTTGCATATTACTTCCTTCGCCAAAACCTAAGAGAAATTGCTAAATCCAATGACAATGCAAAATTCATCATCACTGGTCATAGTTTAGGTGGTGCCCTTGCTACCCTTTTTGTCACTTTGTTAGCCTACCACAATGAAACAATCCTTCTTGACAAGATTCAAGCTGTTTATACCTTTGGTCAGCCTAGAGTTGGGAATCAAAGTTTTGCGCAGCTTATGGTCAAAACTTTCAAAACTCATGACATTAAATATTATAGATATGTTTACTCTTTTGATTTGGTTCCTAGAATTCCTTTCGATTCTCTTGCTAATTTCAGCTACAGACACTTTGGTGGATGCGTCTACTTTGATGTCTTCTACAATGGCAAGTTTTTGAAGGAGCAGCCAAACACAAACTACTTTTCACCAATATGGGTTATACCCAAATACTTGAGTGCAGATTGGGAGTTCATTAGAAGTTTAATAATAACCCCAATTGTGAAGGGTAGAAAGTACTTCGAGGGTTTTTTTACAATAATGGAAAGGACAGTTGGATTGGTTATTCCGGGAATATCAGCTCATGTATGTTCAAATTATGTCAACATGACTCGTTGGGGGAATATACAGTTGCCACCTGACCACCACGAACTCTTGAAGTTTGCTCACTACATTGAAGCTGACTACTAG

Coding sequence (CDS)

ATGGGTTCTAACGAGATACAATTGAATGAAAAAGGTCTGATATTAAAGCCAGATAAGGCAACAGTAAGAGATGTATTTGAGTTCTTACTTCCTTCGGGTTCCAGAAAGATAAAAAAGTTGATGGAATGTCCAGACGACCAAATTGGGCTTTACACCAATTTCAAAGCTCGATGGATCATTTCTGTTTCAATTTTGACCCAGAAATTCCTTTTTGCCATCGCTTCTCTTTTCACAATCTTAGTTGCATTTTGGTCAATAATTCAACAGTTTTTCTACAAGTATGTGCTGGCAGCAGAATTTTGGGTTGCGCCATCTCGGATTATTAATTTCACGGATGTGCAGCCCAGAGTGAGACGTAGTGACTGGGAATTGCTGGTTCCCGACGACAATGTAGACATGAATTCACAAGACCGTGATTTCAAATATTATAGTGCCTTAACCATTATGGCTTCCAAATTGGCATATCAAGATTATTCACATTCCTCCTCCCTCGTTGAGTTCGTAGTAAATGACTGCTGGCAGATGAAATTAATTGATTGTCGCAATTTCTGGAACGACTTCCAAAACAAAGCTACGACTCATGCAATTATGTTTGAGAACACAGGCAAAGATCCAAACGTAACAGTGGTTGCATTCAGAGGGACATCCGTACTTGACATTAACGATTGGATGGTTGATCTGAACTTTTCTTGGTTCCTATTAGAAGGCAAGGTCGGCATCCATTCCGGGTTCATGCAAGCCCTTGGCTACCAAAAGTCCGGTGATTGGCCCAAAGAACTCACCGATCCCAAACATGAATTTGCATATTACTTCCTTCGCCAAAACCTAAGAGAAATTGCTAAATCCAATGACAATGCAAAATTCATCATCACTGGTCATAGTTTAGGTGGTGCCCTTGCTACCCTTTTTGTCACTTTGTTAGCCTACCACAATGAAACAATCCTTCTTGACAAGATTCAAGCTGTTTATACCTTTGGTCAGCCTAGAGTTGGGAATCAAAGTTTTGCGCAGCTTATGGTCAAAACTTTCAAAACTCATGACATTAAATATTATAGATATGTTTACTCTTTTGATTTGGTTCCTAGAATTCCTTTCGATTCTCTTGCTAATTTCAGCTACAGACACTTTGGTGGATGCGTCTACTTTGATGTCTTCTACAATGGCAAGTTTTTGAAGGAGCAGCCAAACACAAACTACTTTTCACCAATATGGGTTATACCCAAATACTTGAGTGCAGATTGGGAGTTCATTAGAAGTTTAATAATAACCCCAATTGTGAAGGGTAGAAAGTACTTCGAGGGTTTTTTTACAATAATGGAAAGGACAGTTGGATTGGTTATTCCGGGAATATCAGCTCATGTATGTTCAAATTATGTCAACATGACTCGTTGGGGGAATATACAGTTGCCACCTGACCACCACGAACTCTTGAAGTTTGCTCACTACATTGAAGCTGACTACTAG

Protein sequence

MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWIISVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPRVRRSDWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGIHSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLVPRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPPDHHELLKFAHYIEADY*
Homology
BLAST of Chy7G129690 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 8.6e-66
Identity = 163/510 (31.96%), Postives = 252/510 (49.41%), Query Frame = 0

Query: 2   GSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKA----- 61
           GSN  Q++   LI++P +    D+F + +        K +E PD++              
Sbjct: 10  GSNPGQVSNY-LIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDH 69

Query: 62  RWIISVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLA-AEFWVAPSRIINFTDVQP 121
           RW+I VSIL +K    I  L    + F   +  FF     A   F+    R+I    V P
Sbjct: 70  RWVIVVSILVRK----IIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIP 129

Query: 122 R-----------------VRRSDWELLVPDDNVDMNSQDR-----DFKYYSALTIMASKL 181
                                 +W  +   + +D     R       +    L +MASKL
Sbjct: 130 ERGSVTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKL 189

Query: 182 AYQDYSHSSSLVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFR 241
           AY+    ++ +VE VV+  W+M L++  + WND+Q + +T   +F +  KD N+ V++FR
Sbjct: 190 AYE----NAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFR 249

Query: 242 GTSVLDINDWMVDLNFSWFLLEGKVGIHSGFMQALGYQKSGDW----------------- 301
           GT   D +DW  D ++SW+ +     +H GF++A+G     D                  
Sbjct: 250 GTEPFDADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEEN 309

Query: 302 -PKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETIL 361
             K L D     AYY +R  L+ +   ++NA+F++TGHSLGGALA LF TLL  + ET +
Sbjct: 310 SKKNLLDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEI 369

Query: 362 LDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLVPRIPFDSLANFSYRH 421
           + ++  VYTFGQPR+GN+     M         +Y+R VY  D+VPR+P+D    F Y+H
Sbjct: 370 MKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDD-KTFLYKH 429

Query: 422 FGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGF 466
           FG C+++D FYN    +++P+ N +   + I  ++ A WE +R L +     G  Y EG+
Sbjct: 430 FGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTM-GYTHGPDYKEGW 489

BLAST of Chy7G129690 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 6.4e-61
Identity = 132/379 (34.83%), Postives = 198/379 (52.24%), Query Frame = 0

Query: 146 LTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTGKDP 205
           L +MASKLAY+    +  +V  VVN  W+M  +D  N WNDF+ + +T   +  +  KD 
Sbjct: 190 LCMMASKLAYE----NEKVVRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDA 249

Query: 206 NVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGIHSGFMQALGY--------------- 265
           N+ +V+FRGT   D +DW+ D ++SW+ +     +H GF++ALG                
Sbjct: 250 NLILVSFRGTEPFDADDWITDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFV 309

Query: 266 ------------------------------------QKSGDWPKELTDPKHEF------- 325
                                                 S D  +     K +F       
Sbjct: 310 NNLKFANLENVHATIPPSESSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPER 369

Query: 326 -AYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFG 385
            AYY +R  L+ + K + NAKF++TGHSLGGALA LF  +L  H E  +++++  +YT+G
Sbjct: 370 TAYYVVRSKLKRLLKEHKNAKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYG 429

Query: 386 QPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLVPRIPFDSLANFSYRHFGGCVYFDVFY 445
           QPRVGN+   + M    +    KY+R VY  DLVPR+P+D+   F ++HFG C Y++  Y
Sbjct: 430 QPRVGNRQLGRFMEAHLEHPVPKYFRVVYCNDLVPRLPYDN-KTFLFKHFGICQYYNSLY 489

Query: 446 NGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLV 466
             + + E+PN NYF   +++P YL+A WE IRS  +   + G +Y E + ++M R +GL 
Sbjct: 490 IEQNINEEPNPNYFGMRFLVPLYLNAGWELIRSFTM-GYMYGSEYEECWESVMLRALGLF 549

BLAST of Chy7G129690 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 1.6e-59
Identity = 165/506 (32.61%), Postives = 250/506 (49.41%), Query Frame = 0

Query: 13  LILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWIISVSILTQKFLFA 72
           LI+ PD+ T  D+F+ ++ S      K     D ++     + +RW +SV I     LF 
Sbjct: 13  LIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWPVSVFICKILQLFQ 72

Query: 73  IASLFTILVAFWSIIQQFFYKY-----VLAAEFWV---APSR-------IINFTDVQ--- 132
           + +   +L      +  F+Y+      +L   F +    P R        I + D +   
Sbjct: 73  MPA--AMLGHLTDFLLNFYYQNHGFLGILRNIFLIRLKIPKRGEADFISTIGYLDSRMDL 132

Query: 133 ---PRVRRSDWELLVPDDNVDMNSQDRDFKYYSA--------LTIMASKLAYQDYSHSSS 192
              P V     E++   DN  +  +  + K   A        L IMASKLAY+    ++ 
Sbjct: 133 HGTPMVSHQADEVISNADNPSL-KEGHNSKIKGALGNRSLMDLCIMASKLAYE----NTK 192

Query: 193 LVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDW 252
           +VE VV + W+M  +      N FQ+   THA +F +  KD N+ V++FRGT    I +W
Sbjct: 193 VVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIPNW 252

Query: 253 MVDLNFSWFLLEGKVGIHSGFMQA--LGYQKS---------GDWPKELTD--------PK 312
             D +FS   L     +H GF++A  LG++ S            P  +T+        P 
Sbjct: 253 CTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMAPD 312

Query: 313 H-----EFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDKI 372
           H     +  Y+     L+ + K + NAKF++TGHSLGGALA LF  +L    ET +LD++
Sbjct: 313 HLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLDRL 372

Query: 373 QAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLVPRIPFDSLANFSYRHFGGC 432
             VYTFGQPR+GN +    M       + +Y+R VY  D+VPR+PFD +  F++ HFG C
Sbjct: 373 LNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVF-FTFEHFGTC 432

Query: 433 VYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGFFTIM 466
           +Y+D  + G F KE+P+ N F     I  +++A WE  RS I+   V G +Y E + + M
Sbjct: 433 IYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNH-VYGAEYKETWESRM 492

BLAST of Chy7G129690 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 8.7e-10
Identity = 72/248 (29.03%), Postives = 118/248 (47.58%), Query Frame = 0

Query: 132 DMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDCRNFWNDFQNKA 191
           D+ +Q   F  YSA    A+     + + + + +    N C +++  D   F   F++  
Sbjct: 27  DLFNQFNLFAQYSA----AAYCGKNNDAPAGTNITCTGNACPEVEKADA-TFLYSFEDSG 86

Query: 192 ---TTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGIHSGFMQAL 251
               T  +  +NT K   + V++FRG+    I +W+ +LNF    L+    I SG     
Sbjct: 87  VGDVTGFLALDNTNK---LIVLSFRGSR--SIENWIGNLNFD---LKEINDICSGCRGHD 146

Query: 252 GYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLA 311
           G+  S  W + + D         LRQ + +  + + + + + TGHSLGGALAT+    L 
Sbjct: 147 GFTSS--W-RSVADT--------LRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLR 206

Query: 312 YHNETILLDKIQAVYTFGQPRVGNQSFAQ-LMVKTFKTHDIKYYRYVYSFDLVPRIPFDS 371
            +   I       V+++G PRVGN++FA+ L V+T  T     YR  ++ D+VPR+P   
Sbjct: 207 GNGYDI------DVFSYGAPRVGNRAFAEFLTVQTGGT----LYRITHTNDIVPRLP--- 237

Query: 372 LANFSYRH 376
              F Y H
Sbjct: 267 PREFGYSH 237

BLAST of Chy7G129690 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 66.2 bits (160), Expect = 1.1e-09
Identity = 59/179 (32.96%), Postives = 80/179 (44.69%), Query Frame = 0

Query: 202 GKDPNVTVVAFRGTSVLDINDWMVDLNF---SWFLLEGKVGIHSGFMQALGYQKSGDWPK 261
           G       + FRG+S   I +W+ DL F   S+  + G   +H GF+ + G         
Sbjct: 163 GDSEKTIYIVFRGSS--SIRNWIADLTFVPVSYPPVSG-TKVHKGFLDSYG--------- 222

Query: 262 ELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETILLDK 321
              + ++E     L Q      K   + K  +TGHSLGGA A L   L  Y  E  L   
Sbjct: 223 ---EVQNELVATVLDQ-----FKQYPSYKVAVTGHSLGGATA-LLCALDLYQREEGLSSS 282

Query: 322 IQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLVPRIPFDSLANFSYRHFG 378
              +YT GQPRVG+ +FA  +V T     I Y R V   D+VP +P    A F + H G
Sbjct: 283 NLFLYTQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLP---PAAFGFLHAG 313

BLAST of Chy7G129690 vs. ExPASy TrEMBL
Match: A0A0A0KZS7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639880 PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.7e-274
Identity = 462/487 (94.87%), Postives = 468/487 (96.10%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MGSNEIQLNE GL LKPDKA VRDV EFLLPSGSRKIKKLMECPDDQI LYTNFKARW I
Sbjct: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPRVRRS 120
           SVSILTQKFL AIASLFTILVAFWSIIQ+F YKYVLAAEFWVAPSRIINFTDVQPRVR S
Sbjct: 61  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120

Query: 121 DWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDC 180
           DWELLVPDDN DMNS DRDFKYYSALTIMASKLAYQDYS S+S+VEFVVNDCWQMKLIDC
Sbjct: 121 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180

Query: 181 RNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGI 240
           RNFWNDFQNKATTHAIMFENT KDPNVTV+AFRGTSVLDINDWMVDL+FSWFLLEGKVGI
Sbjct: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240

Query: 241 HSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           HSGFMQALGYQKSG WPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA
Sbjct: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQ MV TFKTHDIKYYRYVYSFDLV
Sbjct: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360

Query: 361 PRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSL 420
           PRIPF SLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFS IWVIPKYLSADWEFIRSL
Sbjct: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 420

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPPDHHELLKFA 480
           IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVN+TRWGNI LPPDHHELLKFA
Sbjct: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 480

Query: 481 HYIEADY 488
           HYIEADY
Sbjct: 481 HYIEADY 487

BLAST of Chy7G129690 vs. ExPASy TrEMBL
Match: A0A6J1FDN5 (uncharacterized protein LOC111443149 OS=Cucurbita moschata OX=3662 GN=LOC111443149 PE=4 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 1.4e-172
Identity = 302/487 (62.01%), Postives = 366/487 (75.15%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MGS + + +E  LILKP +A++ DVF F+LP GSR+I+ L++CPD +   Y++FKAR  I
Sbjct: 3   MGSKQNEFSEDYLILKPKEASLVDVFLFILPLGSRRIRSLIDCPDAKEESYSSFKARCTI 62

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLA-AEFWVAPSRIINFTDVQPRVRR 120
            VSIL QKFL A+A+    L AF S+IQ  F+ Y++   EF+         +D  P VR 
Sbjct: 63  FVSILVQKFLLALAAPLLTLRAFLSVIQPLFFNYMIGKGEFYTR-------SDSGPEVRC 122

Query: 121 SDWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLID 180
            DW++  PD        D+ FKYY ALT+MAS+LAY+DY+ S S+V  VVN CWQM L+ 
Sbjct: 123 RDWQIEDPD--------DKGFKYYGALTMMASQLAYEDYTASPSVVHSVVNGCWQMTLLG 182

Query: 181 CRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVG 240
           C NFWNDFQNKATT A MF+NT KDPNVTVVAFRGTSV + +DWMVD N SW+ +EG   
Sbjct: 183 CFNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGR 242

Query: 241 IHSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGAL 300
           IHSGFMQALG QK+  WPKEL + +HEFAYY LR+ LR+I KSND AKFIITGHSLGGAL
Sbjct: 243 IHSGFMQALGLQKATGWPKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITGHSLGGAL 302

Query: 301 ATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDL 360
           ATLFVTLLA H ++ +L ++QAVYTFGQPRVG++ FA+ MV T   + +KYYRYVYSFDL
Sbjct: 303 ATLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYRYVYSFDL 362

Query: 361 VPRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRS 420
           VPR+PFDS+  F Y+HFGGC+YFD FYNGKF KEQPNTNYFS IWVIPKY SA WE I S
Sbjct: 363 VPRVPFDSII-FKYKHFGGCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAHWELISS 422

Query: 421 LIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPPDHHELLKF 480
           LII P+ KGR+YFEGF T +ER VGL  PG+SAH+C NYVN+TRWG I++ PD +E LK 
Sbjct: 423 LIIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETLKL 473

Query: 481 AHYIEAD 487
             YIEAD
Sbjct: 483 VRYIEAD 473

BLAST of Chy7G129690 vs. ExPASy TrEMBL
Match: A0A6J1CEU2 (uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010582 PE=4 SV=1)

HSP 1 Score: 516.9 bits (1330), Expect = 9.0e-143
Identity = 259/466 (55.58%), Postives = 322/466 (69.10%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MGS E + +   LILKP++AT+  +F F LP G RK++ L++CP  +   YT F  RW+I
Sbjct: 1   MGSQENKFSSDYLILKPEEATLLHLFLFTLPFGYRKLRNLVDCPVAKENSYTTFGNRWVI 60

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPRVRRS 120
            +SIL QKFL AIA+    L+ +   ++  F+K                 T   P+V   
Sbjct: 61  FISILLQKFLLAIAN----LIKYCKSMRGKFHK-----------------TCAAPKVNCK 120

Query: 121 DWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDY-SHSSSLVEFVVNDCWQMKLID 180
           +W+++  + N+ + + D  FKYY ALT+MAS LAYQDY S   S+V+ VVN CW+M L+ 
Sbjct: 121 EWKIIGMEKNIKL-ADDDTFKYYGALTVMASNLAYQDYNSPEPSVVQTVVNGCWKMNLLG 180

Query: 181 CRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVG 240
           C +FWNDFQNKATT   MF+NT  DPNVTVVAFRG+S  +I DWMVDLN SW+ +EG   
Sbjct: 181 CYDFWNDFQNKATTQVFMFQNTATDPNVTVVAFRGSS--EIYDWMVDLNLSWYNIEGIGK 240

Query: 241 IHSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGAL 300
           IH GFMQALG QK   WPKEL +P H+FAYY LRQ LR+I KSNDNA+FI TGHSLGGAL
Sbjct: 241 IHGGFMQALGLQKGTGWPKELANPDHDFAYYTLRQQLRDIVKSNDNARFIFTGHSLGGAL 300

Query: 301 ATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDL 360
           A LF TLLA+H++  LL+K+QAVYTFGQPRVG++ FAQ M  T + +  KYYRYVYS DL
Sbjct: 301 AVLFATLLAFHDDATLLEKLQAVYTFGQPRVGDRRFAQFMENTIEKYGFKYYRYVYSSDL 360

Query: 361 VPRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRS 420
           VPR+PFD   N  Y HFGGCVYF+  YNGKFL+ QPN NYFSPIW+I KY+SA WE IRS
Sbjct: 361 VPRVPFDFNDNLWYEHFGGCVYFNCSYNGKFLEVQPNKNYFSPIWLISKYVSAWWELIRS 420

Query: 421 LIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWG 466
           L+I  I     Y EGF  ++ R  GL+ PG SAH+C NY+N  RWG
Sbjct: 421 LMIPVIKCSSDYLEGFSNLLLRLFGLLFPGASAHMCLNYINSIRWG 442

BLAST of Chy7G129690 vs. ExPASy TrEMBL
Match: A0A6J1C148 (uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 1.0e-130
Identity = 248/474 (52.32%), Postives = 329/474 (69.41%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MG  + Q +   LILKP++AT+ D+F F+LP G  +++KL++CP+++   Y +F  RW+I
Sbjct: 1   MGPEDYQFSNDYLILKPEEATLLDLFLFILPFGYLRLRKLVDCPEEKEESYKSFGNRWVI 60

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPRVRRS 120
            +SIL+QKFL AIA+    L+  W           +  +FW    R+  +      V   
Sbjct: 61  FISILSQKFLLAIAN----LLEKWE---------PMRGKFW----RVQTYAS---EVNCR 120

Query: 121 DWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYS-HSSSLVEFVVNDCWQMKLID 180
           DW+ +  ++N+ + + D  F YY A+T+MAS L YQD S   SS+V  VVN CW+MKL+ 
Sbjct: 121 DWK-IGKEENIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKLLG 180

Query: 181 CRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVG 240
           C +F N F++KA T A MF+NT  DPNVTVVAF+GT+   I D ++DLNFSW+ ++ ++G
Sbjct: 181 CYDFENMFESKARTQATMFQNTATDPNVTVVAFKGTNT--IYDAILDLNFSWYNVDQRIG 240

Query: 241 -IHSGFMQALGYQKSG-DWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGG 300
            IHSGFM+ALG QK    WPKEL    H+FAYY+LRQ LR+IAKSND AKFI TGHSLGG
Sbjct: 241 NIHSGFMEALGLQKGTIGWPKELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSLGG 300

Query: 301 ALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSF 360
           ALA LFVT+L+YHNE+ +L+K+QAVYTFGQPRVGN  F + M  T K +  KYYRYVYS 
Sbjct: 301 ALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVYSM 360

Query: 361 DLVPRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFI 420
           DLVPRIPFD    + Y+HFGGCVYF+  Y+G+F++ QPN NYFSP+W+IPKYL A WE I
Sbjct: 361 DLVPRIPFDIFDEW-YKHFGGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWELI 420

Query: 421 RSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPP 472
           RSL++  I     YFEGF T++ R  GL+IPG SAH+C NY+ + R G  ++PP
Sbjct: 421 RSLMMPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQG--KMPP 448

BLAST of Chy7G129690 vs. ExPASy TrEMBL
Match: A0A6J1C321 (uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111007483 PE=4 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 8.8e-130
Identity = 249/476 (52.31%), Postives = 324/476 (68.07%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MGS E + +   L LKP +AT+ D+F FLLP GS K++KL++CP ++   YT+F  RW+I
Sbjct: 1   MGSLEYEFSNDYLKLKPKEATLLDLFIFLLPFGSIKLRKLVDCPPEKEESYTSFTNRWVI 60

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFT--DVQPRVR 120
             SIL+QK LFA+A+L                      E W + +  +          V 
Sbjct: 61  FFSILSQKSLFALANLL---------------------EKWESSTEKLRTAPQTYASEVN 120

Query: 121 RSDWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYS-HSSSLVEFVVNDCWQMKL 180
             DW+ +  ++N+ + + D  F YY A+T+MAS L YQD S   SS+V  VVN CW+MKL
Sbjct: 121 CRDWK-IGKEENIKLLNDDDTFVYYGAVTVMASNLVYQDCSPPESSVVATVVNSCWKMKL 180

Query: 181 IDCRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGK 240
           + C +F N F++KA T A MF+NT  DPNVTVVAF+GT+   I D ++DLNFSW+ ++ +
Sbjct: 181 LGCYDFENMFESKARTQATMFQNTATDPNVTVVAFKGTNT--IYDAILDLNFSWYNVDQR 240

Query: 241 VG-IHSGFMQALGYQKSG-DWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSL 300
           +G IHSGFM+ALG QK    WPKEL    H+FAYY+LRQ LR+IAKSND AKFI TGHSL
Sbjct: 241 IGNIHSGFMEALGLQKGTIGWPKELPKSDHQFAYYYLRQQLRDIAKSNDKAKFIFTGHSL 300

Query: 301 GGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVY 360
           GGALA LFVT+L+YHNE+ +L+K+QAVYTFGQPRVGN  F + M  T K +  KYYRYVY
Sbjct: 301 GGALAILFVTILSYHNESDVLNKLQAVYTFGQPRVGNSQFVKFMESTSKQYAFKYYRYVY 360

Query: 361 SFDLVPRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWE 420
           S DLVPRIPFD    + Y+HFGGCVYF+  Y+G+F++ QPN NYFSP+W+IPKYL A WE
Sbjct: 361 SMDLVPRIPFDIFDEW-YKHFGGCVYFNCCYSGQFMEVQPNKNYFSPLWLIPKYLFALWE 420

Query: 421 FIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPP 472
            IRSL++  I     YFEGF T++ R  GL+IPG SAH+C NY+ + R G  ++PP
Sbjct: 421 LIRSLMMPLIKFSLYYFEGFKTLLLRFFGLLIPGASAHLCVNYIYLARQG--KMPP 449

BLAST of Chy7G129690 vs. NCBI nr
Match: XP_031739748.1 (uncharacterized protein LOC105435389 [Cucumis sativus])

HSP 1 Score: 948 bits (2451), Expect = 0.0
Identity = 462/487 (94.87%), Postives = 468/487 (96.10%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MGSNEIQLNE GL LKPDKA VRDV EFLLPSGSRKIKKLMECPDDQI LYTNFKARW I
Sbjct: 12  MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 71

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPRVRRS 120
           SVSILTQKFL AIASLFTILVAFWSIIQ+F YKYVLAAEFWVAPSRIINFTDVQPRVR S
Sbjct: 72  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 131

Query: 121 DWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDC 180
           DWELLVPDDN DMNS DRDFKYYSALTIMASKLAYQDYS S+S+VEFVVNDCWQMKLIDC
Sbjct: 132 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 191

Query: 181 RNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGI 240
           RNFWNDFQNKATTHAIMFENT KDPNVTV+AFRGTSVLDINDWMVDL+FSWFLLEGKVGI
Sbjct: 192 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 251

Query: 241 HSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           HSGFMQALGYQKSG WPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA
Sbjct: 252 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 311

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQ MV TFKTHDIKYYRYVYSFDLV
Sbjct: 312 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 371

Query: 361 PRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSL 420
           PRIPF SLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFS IWVIPKYLSADWEFIRSL
Sbjct: 372 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 431

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPPDHHELLKFA 480
           IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVN+TRWGNI LPPDHHELLKFA
Sbjct: 432 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 491

Query: 481 HYIEADY 487
           HYIEADY
Sbjct: 492 HYIEADY 498

BLAST of Chy7G129690 vs. NCBI nr
Match: KGN55200.1 (hypothetical protein Csa_012841 [Cucumis sativus])

HSP 1 Score: 948 bits (2451), Expect = 0.0
Identity = 462/487 (94.87%), Postives = 468/487 (96.10%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MGSNEIQLNE GL LKPDKA VRDV EFLLPSGSRKIKKLMECPDDQI LYTNFKARW I
Sbjct: 1   MGSNEIQLNENGLTLKPDKAKVRDVLEFLLPSGSRKIKKLMECPDDQIELYTNFKARWTI 60

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPRVRRS 120
           SVSILTQKFL AIASLFTILVAFWSIIQ+F YKYVLAAEFWVAPSRIINFTDVQPRVR S
Sbjct: 61  SVSILTQKFLSAIASLFTILVAFWSIIQKFCYKYVLAAEFWVAPSRIINFTDVQPRVRCS 120

Query: 121 DWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDC 180
           DWELLVPDDN DMNS DRDFKYYSALTIMASKLAYQDYS S+S+VEFVVNDCWQMKLIDC
Sbjct: 121 DWELLVPDDNADMNSHDRDFKYYSALTIMASKLAYQDYSRSASIVEFVVNDCWQMKLIDC 180

Query: 181 RNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGI 240
           RNFWNDFQNKATTHAIMFENT KDPNVTV+AFRGTSVLDINDWMVDL+FSWFLLEGKVGI
Sbjct: 181 RNFWNDFQNKATTHAIMFENTHKDPNVTVIAFRGTSVLDINDWMVDLDFSWFLLEGKVGI 240

Query: 241 HSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           HSGFMQALGYQKSG WPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA
Sbjct: 241 HSGFMQALGYQKSGGWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQ MV TFKTHDIKYYRYVYSFDLV
Sbjct: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQFMVDTFKTHDIKYYRYVYSFDLV 360

Query: 361 PRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSL 420
           PRIPF SLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFS IWVIPKYLSADWEFIRSL
Sbjct: 361 PRIPFHSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSLIWVIPKYLSADWEFIRSL 420

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPPDHHELLKFA 480
           IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVN+TRWGNI LPPDHHELLKFA
Sbjct: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNLTRWGNIHLPPDHHELLKFA 480

Query: 481 HYIEADY 487
           HYIEADY
Sbjct: 481 HYIEADY 487

BLAST of Chy7G129690 vs. NCBI nr
Match: XP_038896694.1 (triacylglycerol lipase OBL1-like [Benincasa hispida])

HSP 1 Score: 756 bits (1951), Expect = 1.08e-271
Identity = 364/487 (74.74%), Postives = 413/487 (84.80%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MGSNEI+  E  LILK   A VRDVF FLLPSGSRKIK ++ CPDDQIG YTNFKARW I
Sbjct: 1   MGSNEIEFRENYLILKAKDAKVRDVFLFLLPSGSRKIKNVIYCPDDQIGFYTNFKARWTI 60

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPRVRRS 120
           SVSIL QKFL  +A+L T+L AFWSIIQ+FFYKY+L AEFWVAP  I NF D++P+VR  
Sbjct: 61  SVSILAQKFLLTLATLLTLLAAFWSIIQRFFYKYILLAEFWVAPQTI-NFMDLKPKVRCR 120

Query: 121 DWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDC 180
           DW++L P+DN DMN ++RDF+YYS LTIMASKLAYQDYS   S+VE VVNDCWQM LIDC
Sbjct: 121 DWKILDPNDNADMNLEERDFRYYSNLTIMASKLAYQDYSQYPSVVESVVNDCWQMNLIDC 180

Query: 181 RNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGI 240
            NFWNDFQ+KATTHA MFENT KDP VTVVAFRGTSVL+ +DW VD NFSW  LEG   I
Sbjct: 181 FNFWNDFQDKATTHAFMFENTSKDPKVTVVAFRGTSVLEPSDWTVDFNFSWSKLEGVGNI 240

Query: 241 HSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           H+GFMQALG+Q    WPK+L  PKH++AYY LRQ L++IAK+NDNA+FIITGHSLGGALA
Sbjct: 241 HAGFMQALGFQDLAGWPKDLLQPKHQYAYYTLRQKLKDIAKANDNAEFIITGHSLGGALA 300

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLA+HNET +L KI+AVYT+GQPRVGNQ FAQ MV T + +  KYYRYVYSFDLV
Sbjct: 301 TLFVTLLAHHNETTILHKIRAVYTYGQPRVGNQEFAQFMVDTVERYGFKYYRYVYSFDLV 360

Query: 361 PRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSL 420
           PR+PFDS+  F Y+HFGGCVYFD FY+GKFLKEQPNTNYFSPIW+IPKYLSA WEFIRSL
Sbjct: 361 PRVPFDSVV-FKYKHFGGCVYFDCFYHGKFLKEQPNTNYFSPIWLIPKYLSACWEFIRSL 420

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPPDHHELLKFA 480
           IITPIVKGR YFEGFFTI+ERT+GL+IPGISAHVCSNYVN+TR GNIQLP D+ E L+ A
Sbjct: 421 IITPIVKGRDYFEGFFTILERTLGLLIPGISAHVCSNYVNVTRLGNIQLPSDYEETLQLA 480

Query: 481 HYIEADY 487
           HYI+A+Y
Sbjct: 481 HYIDANY 485

BLAST of Chy7G129690 vs. NCBI nr
Match: XP_022936593.1 (uncharacterized protein LOC111443149 [Cucurbita moschata])

HSP 1 Score: 610 bits (1573), Expect = 2.23e-214
Identity = 300/486 (61.73%), Postives = 363/486 (74.69%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MGS + + +E  LILKP +A++ DVF F+LP GSR+I+ L++CPD +   Y++FKAR  I
Sbjct: 3   MGSKQNEFSEDYLILKPKEASLVDVFLFILPLGSRRIRSLIDCPDAKEESYSSFKARCTI 62

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPRVRRS 120
            VSIL QKFL A+A+    L AF S+IQ  F+ Y+      +        +D  P VR  
Sbjct: 63  FVSILVQKFLLALAAPLLTLRAFLSVIQPLFFNYM------IGKGEFYTRSDSGPEVRCR 122

Query: 121 DWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDC 180
           DW++  PD        D+ FKYY ALT+MAS+LAY+DY+ S S+V  VVN CWQM L+ C
Sbjct: 123 DWQIEDPD--------DKGFKYYGALTMMASQLAYEDYTASPSVVHSVVNGCWQMTLLGC 182

Query: 181 RNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGI 240
            NFWNDFQNKATT A MF+NT KDPNVTVVAFRGTSV + +DWMVD N SW+ +EG   I
Sbjct: 183 FNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGRI 242

Query: 241 HSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           HSGFMQALG QK+  WPKEL + +HEFAYY LR+ LR+I KSND AKFIITGHSLGGALA
Sbjct: 243 HSGFMQALGLQKATGWPKELPEAEHEFAYYTLRRRLRDIVKSNDKAKFIITGHSLGGALA 302

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLA H ++ +L ++QAVYTFGQPRVG++ FA+ MV T   + +KYYRYVYSFDLV
Sbjct: 303 TLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGLKYYRYVYSFDLV 362

Query: 361 PRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSL 420
           PR+PFDS+  F Y+HFGGC+YFD FYNGKF KEQPNTNYFS IWVIPKY SA WE I SL
Sbjct: 363 PRVPFDSII-FKYKHFGGCLYFDCFYNGKFRKEQPNTNYFSLIWVIPKYSSAHWELISSL 422

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPPDHHELLKFA 480
           II P+ KGR+YFEGF T +ER VGL  PG+SAH+C NYVN+TRWG I++ PD +E LK  
Sbjct: 423 IIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETLKLV 473

Query: 481 HYIEAD 486
            YIEAD
Sbjct: 483 RYIEAD 473

BLAST of Chy7G129690 vs. NCBI nr
Match: KAG6591383.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 610 bits (1572), Expect = 3.16e-214
Identity = 300/486 (61.73%), Postives = 362/486 (74.49%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           MGS + + +E  LILKP +A++ DVF F+LP GSR+I+ L++CPD +   Y++FKAR  I
Sbjct: 3   MGSKQNEFSEDYLILKPKEASLVDVFLFILPLGSRRIRSLIDCPDAKEESYSSFKARCTI 62

Query: 61  SVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPRVRRS 120
            VSIL QKFL A+A+    L AF S+IQ  F+ Y+      +        +D  P VR  
Sbjct: 63  FVSILVQKFLLALAAPLLTLRAFLSVIQPLFFNYM------IGKGEFYTRSDSGPEVRCR 122

Query: 121 DWELLVPDDNVDMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDC 180
           DW++  PD        D+ FKYY ALT+MAS+LAY+DY+ S S+V  VVN CWQM L+ C
Sbjct: 123 DWQIEDPD--------DKGFKYYGALTMMASQLAYEDYTASPSVVHSVVNGCWQMTLLGC 182

Query: 181 RNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGI 240
            NFWNDFQNKATT A MF+NT KDPNVTVVAFRGTSV + +DWMVD N SW+ +EG   I
Sbjct: 183 FNFWNDFQNKATTGAFMFQNTAKDPNVTVVAFRGTSVFEASDWMVDFNISWYKIEGIGRI 242

Query: 241 HSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALA 300
           HSGFMQALG QK+  WPKEL   +HEFAYY LRQ LR+I KSND AKFI+TGHSLGGALA
Sbjct: 243 HSGFMQALGLQKATGWPKELPGAEHEFAYYTLRQRLRDIVKSNDKAKFIMTGHSLGGALA 302

Query: 301 TLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLV 360
           TLFVTLLA H ++ +L ++QAVYTFGQPRVG++ FA+ MV T   +  KYYRYVYSFDLV
Sbjct: 303 TLFVTLLALHQDSTILQRLQAVYTFGQPRVGDKDFAEFMVNTVTRYGFKYYRYVYSFDLV 362

Query: 361 PRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSL 420
           PR+PFDS+  F Y+HFGGC+YFD FYNGKF +EQPNTNYFS IWVIPKYLSA WE I SL
Sbjct: 363 PRVPFDSII-FKYKHFGGCLYFDCFYNGKFRREQPNTNYFSLIWVIPKYLSAHWELISSL 422

Query: 421 IITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPPDHHELLKFA 480
           II P+ KGR+YFEGF T +ER VGL  PG+SAH+C NYVN+TRWG I++ PD +E LK  
Sbjct: 423 IIIPVFKGREYFEGFVTTLERVVGLFFPGLSAHICPNYVNVTRWGKIKVLPDRNETLKLV 473

Query: 481 HYIEAD 486
            YIEAD
Sbjct: 483 RYIEAD 473

BLAST of Chy7G129690 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 326.2 bits (835), Expect = 4.3e-89
Identity = 191/485 (39.38%), Postives = 284/485 (58.56%), Query Frame = 0

Query: 14  ILKPDKATVRDVFEFLLPS--GSRK-IKKLMECPDDQIGLYTNFKARWIISVSILTQKFL 73
           +L P +ATV D+   L  S  G RK I    E  +D +   + F+ RWII VSI+ QK +
Sbjct: 9   VLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDL---SRFRRRWIIFVSIVIQKLI 68

Query: 74  -----------FAIASLFTILVA---FWSIIQQFFYKYVLAAEFWVAPSRIINFTDVQPR 133
                      F++A    +L +   F  I+   F   ++  E   A    +N       
Sbjct: 69  ILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLNGN----- 128

Query: 134 VRRSDWELLVPDDNVDMNSQ-DRDFKYYSA-LTIMASKLAYQDYSHSSSLVEFVVNDCWQ 193
                      D  V++N + +R  K Y A L+IMASKL+Y++ +  SS    V+++ W+
Sbjct: 129 ----------LDRRVELNPKVERGSKRYKAMLSIMASKLSYENINFVSS----VLHNHWK 188

Query: 194 MKLIDCRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLL 253
           M L+   + WN +Q + +T  I+ ++T  DPN+ +V+FRGT   D +DW  DL+ SW+ +
Sbjct: 189 MDLLGFYSCWNGYQKQRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEV 248

Query: 254 EGKVGIHSGFMQALGYQKSGDWPKELTDPKHE-----FAYYFLRQNLREIAKSNDNAKFI 313
           +    IH GFM+ALG QK G WPKE+   + +     +AYY +R++L+EI   N  +KFI
Sbjct: 249 KNVGKIHGGFMKALGLQKEG-WPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFI 308

Query: 314 ITGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIK 373
           +TGHSLGGALA LF  +L  H+E  +L++++ VYTFGQPRVG++ F   M  + K  D+K
Sbjct: 309 LTGHSLGGALAILFTAVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVK 368

Query: 374 YYRYVYSFDLVPRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKY 433
           Y RYVY  D+VPR+PFD      ++HFG C+Y+D FY GK  +E+PN NYF+ +WV+PK 
Sbjct: 369 YERYVYCNDMVPRLPFDD-KTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKI 428

Query: 434 LSADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQL 475
           ++A WE IRS ++ P  KG ++ EG+F    R V L+IPG+ AH  + Y+N+T  G++  
Sbjct: 429 MNALWELIRSFVM-PYWKGGEFREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLGDL-- 465

BLAST of Chy7G129690 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 321.2 bits (822), Expect = 1.4e-87
Identity = 176/481 (36.59%), Postives = 270/481 (56.13%), Query Frame = 0

Query: 1   MGSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWII 60
           M    ++      ++ P KA+  D+   L  S     + +   PD   G   +F +RWI+
Sbjct: 1   MSKTNMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWIL 60

Query: 61  SVSILTQKFLFAIASLFTIL---VAFWSII---QQFFYKYVLAAEFWVAPSRIINFTDVQ 120
           +++I  QK L  ++  F  +   + +W  +      F+  +L          +++   V+
Sbjct: 61  ALAIFLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLIL---------NLMSGKLVK 120

Query: 121 PRVRRSDWELLV--PDDNVDMNSQDR--DFKYYSALTIMASKLAYQDYSHSSSLVEFVVN 180
           P    + +   +   D  ++++ +      +Y S L+IMASK++Y+    S   +  VV 
Sbjct: 121 PDKSSATYTSFIGCSDRRIELDEKINVGSIEYKSMLSIMASKISYE----SKPYITSVVK 180

Query: 181 DCWQMKLIDCRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFS 240
           + W+M L+   +F+N FQ    T A +F+ +  +P++ VV+FRGT   +  DW  DL+ S
Sbjct: 181 NTWKMDLVGNYDFYNAFQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLS 240

Query: 241 WFLLEGKVGIHSGFMQALGYQKSGDWPKELTDPKHEFAYYFLRQNLREIAKSNDNAKFII 300
           W+ ++    +H+GF +ALG QK G WPKE     H++AYY +RQ LR+    N N K+I+
Sbjct: 241 WYEMKNVGKVHAGFSRALGLQKDG-WPKENISLLHQYAYYTIRQMLRDKLGRNKNLKYIL 300

Query: 301 TGHSLGGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKY 360
           TGHSLGGALA LF  +LA H E  LLDK++ +YTFGQPRVG++ F + M    K H I+Y
Sbjct: 301 TGHSLGGALAALFPAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEY 360

Query: 361 YRYVYSFDLVPRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYL 420
            R+VY+ D+VPR+PFD    FSY+H+G C  F+  Y GK  ++ PN NYF+ +W+IP+ L
Sbjct: 361 ERFVYNNDVVPRVPFDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLL 420

Query: 421 SADWEFIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLP 472
           +  WEFIRS I+    KG +Y E +     R VG+V PG S H   +YVN TR G +  P
Sbjct: 421 TGLWEFIRSFIL-QFWKGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLGGLVRP 466

BLAST of Chy7G129690 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 301.2 bits (770), Expect = 1.5e-81
Identity = 180/476 (37.82%), Postives = 258/476 (54.20%), Query Frame = 0

Query: 14  ILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWIISVSILTQKFLFAI 73
           +L P +ATV D+   L  S     K +    ++       F+ RWII VSI+ QK L  +
Sbjct: 9   VLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKLLIIL 68

Query: 74  ASLFTIL---VAFWSIIQQF---FYKYVL---AAEFWVAPSRIINFTDVQPRVRRSDWEL 133
               + L   + FW  +      F+K  L      F         F  +   +       
Sbjct: 69  RKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTSATFASINGNL------- 128

Query: 134 LVPDDNVDM----NSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDC 193
              D  V++    + +  D +Y   L+IMASKLAY+    +   +  V+ D WQM L+  
Sbjct: 129 ---DQKVELGLGRSIKIGDERYKPLLSIMASKLAYE----NEHFIRSVLQDHWQMDLLGF 188

Query: 194 RNFWNDFQNKATTHAIMFENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGI 253
            +  NDF    +T  I+  +T  +PN+ VV+FRGT   + +DW  DL+ SW  +     I
Sbjct: 189 YSCPNDFDQTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKI 248

Query: 254 HSGFMQALGYQKSGDWPKELTDPK-----HEFAYYFLRQNLREIAKSNDNAKFIITGHSL 313
           H GFM+ALG  K G W +E+   +      + AYY + + L+E+ + N  +KFI++GHSL
Sbjct: 249 HGGFMKALGLPKEG-WYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSL 308

Query: 314 GGALATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVY 373
           GGALA LF  +L  H+E  +L++++ VYTFGQPRVG++ F   M    K  D+KY RYVY
Sbjct: 309 GGALAILFTAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVY 368

Query: 374 SFDLVPRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWE 433
             D+VPR+PFD      ++HFGGC+Y D FY GK  +E+PN NYF+  WVIPK ++A WE
Sbjct: 369 CNDMVPRLPFDD-KTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWE 428

Query: 434 FIRSLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTRWGNIQLPP 472
            IRS II+   +GR+Y EG+     R V L+IPG+ AH  + YVN+   GN   PP
Sbjct: 429 LIRSFIIS-CWQGREYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGN--FPP 465

BLAST of Chy7G129690 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 292.0 bits (746), Expect = 9.1e-79
Identity = 170/465 (36.56%), Postives = 264/465 (56.77%), Query Frame = 0

Query: 13  LILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKARWIISVSILTQKFLFA 72
           LIL+P++    ++   L      K + +     ++     +F+ RW+I VS++  K L  
Sbjct: 14  LILRPEELRPWELVRLLFSGDIEKPRSVDSSETEE----HSFRHRWLIFVSLVLLKLLRF 73

Query: 73  IASLFTILVAFWSIIQQFFYKYVLAAEFW----VAPSRIINFTDVQPRVRRSDWELLVPD 132
            + L  ++ +       F      +  F     V P R     + Q  +   D  + +  
Sbjct: 74  FSKLLALVGSALEFSLNFLSNNSFSGLFLRGEVVMPQR--TSENYQSFIGHLDTRVSL-- 133

Query: 133 DNVDMNSQDRDFKYYSALTIMASKLAYQDYSHSSSLVEFVVNDCWQMKLIDCRNFWNDFQ 192
            ++ +N +D + KYY+AL+IMASK+AY+    +++ ++ VV + W MK +   ++WN++Q
Sbjct: 134 -DMTLNREDGE-KYYAALSIMASKIAYE----NAARIKHVVENHWNMKYLGLVDYWNEYQ 193

Query: 193 NKATTHAIMF-------ENTGKDPNVTVVAFRGTSVLDINDWMVDLNFSWFLLEGKVGIH 252
            K TT A +         + G++  V VVAFRGT + +  DW  D + +WF L     IH
Sbjct: 194 EKETTQAFIMSTDETTTRSNGQETTV-VVAFRGTELFNSEDWCSDFDITWFELPNIGNIH 253

Query: 253 SGFMQALGYQKSGDWPKE-LTDP--KHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGA 312
            GFM+ALG Q +  WPKE L++P  K   AYY +R +L+ +   N N KF++TGHSLGGA
Sbjct: 254 GGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGGA 313

Query: 313 LATLFVTLLAYHNETILLDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFD 372
           LA LF  +L  H+ET LL++IQ VYT+GQPRVG+  F + M K  + ++IKYYR+VY+ D
Sbjct: 314 LAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNND 373

Query: 373 LVPRIPFDSLANFSYRHFGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIR 432
           +VPR+P+D   +  ++HFG C+Y+D  Y  K ++EQ + N+F    +I    SA  EFIR
Sbjct: 374 IVPRLPYDD-KDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIR 433

Query: 433 SLIITPIVKGRKYFEGFFTIMERTVGLVIPGISAHVCSNYVNMTR 464
           S  I    KG +Y EG+     R +G+++PG+S H   +YVN TR
Sbjct: 434 SFTIV-AEKGSEYSEGWLLKGGRALGIIVPGVSNHTPQDYVNATR 461

BLAST of Chy7G129690 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 6.1e-67
Identity = 163/510 (31.96%), Postives = 252/510 (49.41%), Query Frame = 0

Query: 2   GSNEIQLNEKGLILKPDKATVRDVFEFLLPSGSRKIKKLMECPDDQIGLYTNFKA----- 61
           GSN  Q++   LI++P +    D+F + +        K +E PD++              
Sbjct: 10  GSNPGQVSNY-LIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDH 69

Query: 62  RWIISVSILTQKFLFAIASLFTILVAFWSIIQQFFYKYVLA-AEFWVAPSRIINFTDVQP 121
           RW+I VSIL +K    I  L    + F   +  FF     A   F+    R+I    V P
Sbjct: 70  RWVIVVSILVRK----IIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIP 129

Query: 122 R-----------------VRRSDWELLVPDDNVDMNSQDR-----DFKYYSALTIMASKL 181
                                 +W  +   + +D     R       +    L +MASKL
Sbjct: 130 ERGSVTFVSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKL 189

Query: 182 AYQDYSHSSSLVEFVVNDCWQMKLIDCRNFWNDFQNKATTHAIMFENTGKDPNVTVVAFR 241
           AY+    ++ +VE VV+  W+M L++  + WND+Q + +T   +F +  KD N+ V++FR
Sbjct: 190 AYE----NAKVVENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFR 249

Query: 242 GTSVLDINDWMVDLNFSWFLLEGKVGIHSGFMQALGYQKSGDW----------------- 301
           GT   D +DW  D ++SW+ +     +H GF++A+G     D                  
Sbjct: 250 GTEPFDADDWGTDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEEN 309

Query: 302 -PKELTDPKHEFAYYFLRQNLREIAKSNDNAKFIITGHSLGGALATLFVTLLAYHNETIL 361
             K L D     AYY +R  L+ +   ++NA+F++TGHSLGGALA LF TLL  + ET +
Sbjct: 310 SKKNLLDMVERSAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEI 369

Query: 362 LDKIQAVYTFGQPRVGNQSFAQLMVKTFKTHDIKYYRYVYSFDLVPRIPFDSLANFSYRH 421
           + ++  VYTFGQPR+GN+     M         +Y+R VY  D+VPR+P+D    F Y+H
Sbjct: 370 MKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDD-KTFLYKH 429

Query: 422 FGGCVYFDVFYNGKFLKEQPNTNYFSPIWVIPKYLSADWEFIRSLIITPIVKGRKYFEGF 466
           FG C+++D FYN    +++P+ N +   + I  ++ A WE +R L +     G  Y EG+
Sbjct: 430 FGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTM-GYTHGPDYKEGW 489

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4JFU88.6e-6631.96Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1[more]
A0A1S3ZP856.4e-6134.83Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1[more]
Q5VKJ71.6e-5932.61Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1[more]
O599528.7e-1029.03Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1[more]
P195151.1e-0932.96Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0KZS71.7e-27494.87Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639880 PE... [more]
A0A6J1FDN51.4e-17262.01uncharacterized protein LOC111443149 OS=Cucurbita moschata OX=3662 GN=LOC1114431... [more]
A0A6J1CEU29.0e-14355.58uncharacterized protein LOC111010582 OS=Momordica charantia OX=3673 GN=LOC111010... [more]
A0A6J1C1481.0e-13052.32uncharacterized protein LOC111007483 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1C3218.8e-13052.31uncharacterized protein LOC111007483 isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
XP_031739748.10.094.87uncharacterized protein LOC105435389 [Cucumis sativus][more]
KGN55200.10.094.87hypothetical protein Csa_012841 [Cucumis sativus][more]
XP_038896694.11.08e-27174.74triacylglycerol lipase OBL1-like [Benincasa hispida][more]
XP_022936593.12.23e-21461.73uncharacterized protein LOC111443149 [Cucurbita moschata][more]
KAG6591383.13.16e-21461.73Lipase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT5G42930.14.3e-8939.38alpha/beta-Hydrolases superfamily protein [more]
AT1G45201.11.4e-8736.59triacylglycerol lipase-like 1 [more]
AT1G56630.11.5e-8137.82alpha/beta-Hydrolases superfamily protein [more]
AT5G67050.19.1e-7936.56alpha/beta-Hydrolases superfamily protein [more]
AT3G14360.16.1e-6731.96alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 128..403
e-value: 1.3E-46
score: 160.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 142..393
IPR002921Fungal lipase-like domainPFAMPF01764Lipase_3coord: 209..366
e-value: 1.1E-34
score: 119.4
NoneNo IPR availablePANTHERPTHR46086:SF4ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 6..470
NoneNo IPR availableCDDcd00519Lipase_3coord: 140..383
e-value: 1.55496E-39
score: 141.075
IPR044819Triacylglycerol lipase OBL1-likePANTHERPTHR46086ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 6..470

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy7G129690.1Chy7G129690.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004806 triglyceride lipase activity