Chy6G123590 (gene) Cucumber (hystrix) v1

Overview
NameChy6G123590
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionribonuclease 3-like protein 2
LocationchrH06: 22078134 .. 22080023 (+)
RNA-Seq ExpressionChy6G123590
SyntenyChy6G123590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCCTCCGTCATAGCCGTTGAGCGCATTCTTTCCTATAACTTTAAGAACAAGAATCTCTTAGAACAAGCCCTCACTCACCCCTCTTACTCTGCCTCTCCCTCCTATGAGCGTCTTGAGTTTGTCGGCGATTCAGCTATTGGCCTTACTATTACTAATTACTTTTTTCTCGCTTATCCCCACCTTCATCAAGGCCATCTCTCTTTACTTAGAGCTGCTAATGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGCGCGATGCACCTGCTATGGATGATAAGGTTTTCAAATTAACCTTCACTTCTCGTTTGTTCCTAAAAATCTTTGATTTTTTTTTGCCCTGTTTTAACTCCATCTCTGTATTTTTTTTTTTATTGTTCAGGTTAAAGAGTTTGTTGATTCCGTTGCCTTAGAAGGTAGCTCAGTTCCTTATGGTGGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGGTGAGTTCTTAGTTACATTTGAAGTATGATTATTATTACTTTTTTAGCTGGGCACCAATTAGTTTTCTTTTCGTCTTCAATCTTTTGTAGATTATTAGGGGTTTGCTTGAGCCTATCTATACTCTTGAAGACTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTCAAATTTGCCAAAGAAATGGGAAGCAGGTTGATATTAAGCATTGGAAAAACGGATCCAAGAGCATCGCGAGTGTCTATGTTGATGGTACTTTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCAAGGCTCAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAGAATAAAAACATTTGTAACTATTGATGGTGTCGATGAATCGTTTGAGATTGAAGGGGCCAAGCAGAAGCTACATGATGCTTGTTGCAAGAGAAAGTGGCAGAAACCAAATTACAGGTATATATATTTATTGTATGATATTTGAGATGTTATACTTGCATTGTTTGGACTCTGAATTAGGAGGTAGTTTATATGTTGTAGTTGTCGATTTTTCATTTAAAACCATGCACCTTAATTATATATTTGCTTTCTAACCAAGTAGGATGCTGAATTTTTCAGTAATTTATAATGAAATGTATTTTTTTCTAAAGCCTGCCTGCTTCAATTGTAATCTTATATCTTCATCGAACTATTTTCATTCTTTCTGTTCATTGTAAAAGTTGCAGCATTTTTTTAAATTTAAGATTCATTTCACTGATCAATGCTCACATATTTTAGAGAAATTACTTTCAGCAATGACAGTGACCTTATCAATTAATTTTAATGTTATTATATCTGTACACTTATTTGTTTAAAAATTATTTTCTTTTGAATCTCCACCCTCTGAGCATACCTTATCAACTTAAGGCCTGTTTCCCTTCCTTTTCCATCTTACACATTGACTTTTGATGTGAATAAAATTCTTCGATATCTGTGCGGATTATCTCGAGTCGTTTTGTGCGGGTCATAAACAAAAGATAAAACTCAGAGAGATCATTTACATACCATGAAGTTATTTGACTATCTTTTTCCCTTCTCCTTGTACTCCTTTTTCAAACGTGTTCTAGTGCTAAATACTTTATGAATTTCTTACTTGCAGTGTGGAGAAAGATCTAGGACCTTCACATGAAAGGAGATTTGTTTGCTCGGTTAAAATTGCCACTTGTTATGGTACGTTCTATATTGTGGGAGATGAGAAATCAAGAGTAAAGGATGCAGAGAACTCGGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCATCTATAA

mRNA sequence

ATGGAATCCTCCGTCATAGCCGTTGAGCGCATTCTTTCCTATAACTTTAAGAACAAGAATCTCTTAGAACAAGCCCTCACTCACCCCTCTTACTCTGCCTCTCCCTCCTATGAGCGTCTTGAGTTTGTCGGCGATTCAGCTATTGGCCTTACTATTACTAATTACTTTTTTCTCGCTTATCCCCACCTTCATCAAGGCCATCTCTCTTTACTTAGAGCTGCTAATGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGCGCGATGCACCTGCTATGGATGATAAGGTTAAAGAGTTTGTTGATTCCGTTGCCTTAGAAGGTAGCTCAGTTCCTTATGGTGGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGATTATTAGGGGTTTGCTTGAGCCTATCTATACTCTTGAAGACTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTCAAATTTGCCAAAGAAATGGGAAGCAGGTTGATATTAAGCATTGGAAAAACGGATCCAAGAGCATCGCGAGTGTCTATGTTGATGGTACTTTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCAAGGCTCAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAGAATAAAAACATTTGTAACTATTGATGGTGTCGATGAATCGTTTGAGATTGAAGGGGCCAAGCAGAAGCTACATGATGCTTGTTGCAAGAGAAAGTGGCAGAAACCAAATTACAGTGTGGAGAAAGATCTAGGACCTTCACATGAAAGGAGATTTGTTTGCTCGGTTAAAATTGCCACTTGTTATGGTACGTTCTATATTGTGGGAGATGAGAAATCAAGAGTAAAGGATGCAGAGAACTCGGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCATCTATAA

Coding sequence (CDS)

ATGGAATCCTCCGTCATAGCCGTTGAGCGCATTCTTTCCTATAACTTTAAGAACAAGAATCTCTTAGAACAAGCCCTCACTCACCCCTCTTACTCTGCCTCTCCCTCCTATGAGCGTCTTGAGTTTGTCGGCGATTCAGCTATTGGCCTTACTATTACTAATTACTTTTTTCTCGCTTATCCCCACCTTCATCAAGGCCATCTCTCTTTACTTAGAGCTGCTAATGTTAGCACTGAGAAGCTTGCTCGTGTTGCAGTCACCCATGGATTGTATAACTATGTTAGGCGCGATGCACCTGCTATGGATGATAAGGTTAAAGAGTTTGTTGATTCCGTTGCCTTAGAAGGTAGCTCAGTTCCTTATGGTGGGTTGATGAAGGCTCCTAAAGTTCTTGCTGACATTGTGGAATCAGTGGCTGGTGCTGTCTATGTAGATGTTAATTTTGATCTTCAAAAACTATGGGTGATTATTAGGGGTTTGCTTGAGCCTATCTATACTCTTGAAGACTTACAAGTAGAGCCCCAACCTGTAACTGTACTCTTTCAAATTTGCCAAAGAAATGGGAAGCAGGTTGATATTAAGCATTGGAAAAACGGATCCAAGAGCATCGCGAGTGTCTATGTTGATGGTACTTTTGTTGCTTCCGGCTCTTCAATGCAAAAAGAAATCGCAAGGCTCAATGCTGCCAGAGAAGCTCTCATCAAATTGTCGGATTCTATGGATACCAGAATAAAAACATTTGTAACTATTGATGGTGTCGATGAATCGTTTGAGATTGAAGGGGCCAAGCAGAAGCTACATGATGCTTGTTGCAAGAGAAAGTGGCAGAAACCAAATTACAGTGTGGAGAAAGATCTAGGACCTTCACATGAAAGGAGATTTGTTTGCTCGGTTAAAATTGCCACTTGTTATGGTACGTTCTATATTGTGGGAGATGAGAAATCAAGAGTAAAGGATGCAGAGAACTCGGCAGCCTCATTGATGATTCGTGCTTTACAGGAAAGAAAGCATCTATAA

Protein sequence

MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAYPHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVPYGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVLFQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSMDTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKIATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL*
Homology
BLAST of Chy6G123590 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 8.8e-102
Identity = 193/337 (57.27%), Postives = 256/337 (75.96%), Query Frame = 0

Query: 4   SVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAYPHL 63
           S+ AVE+IL+Y F NK+LL++A+TH S +  PSYERLEF+GDSAIGL I+NY +L YP L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 64  HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGS-SVPYG 123
               LSLLRAANVSTEKLARV++ HGLY+++RR+AP++D+KVKEF ++V  E   SV YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 124 GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVLFQ 183
           GL+KAPKVLAD+ ES+AGAVYVDVNFDLQ+LWVI RGLLEPI TL+DLQ +PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 184 ICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSMDT 243
           +C ++ K++DIK+WK+G+ SIA +Y+D   +ASG +  K+IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVFPV 298

Query: 244 RIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI-A 303
                + ID      ++  AK KL++ C K+KW KP YSVE+D      +RFVCS KI  
Sbjct: 299 E----MVIDEDSVEIQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 304 TCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 339
           T   T Y+ GDE+S++K AE+S+A  MIRAL++  +L
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRKSHYL 391

BLAST of Chy6G123590 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 256.1 bits (653), Expect = 5.4e-67
Identity = 140/277 (50.54%), Postives = 189/277 (68.23%), Query Frame = 0

Query: 8   VERILSYNFKNKNLLEQALTHPSYS-ASPSYERLEFVGDSAIGLTITNYFFLAYPHLHQG 67
           VER+L Y F++  LLE+ALTH S++  + SY+RLEFVGDSA+GL  +N+ +L  P L  G
Sbjct: 40  VERLLRYQFRDGRLLEEALTHQSFADDAVSYQRLEFVGDSALGLAFSNFLYLTNPTLGPG 99

Query: 68  HLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEG----SSVPYG 127
            LS LRAAN+STEKLARVAV H LY  +RR+ P +D  V +F+++V  E     S+VPYG
Sbjct: 100 PLSTLRAANISTEKLARVAVRHDLYPLLRRNCPRLDLLVGQFIETVKQEPEDDLSTVPYG 159

Query: 128 G-LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVLF 187
           G ++KAPKVLADIVE++A AVYVD  FDL+KLW + R L EPI T E   ++ QPVT+L 
Sbjct: 160 GSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLWKVTRWLFEPIITAE--TIDEQPVTMLH 219

Query: 188 QICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSMD 247
           ++CQ++GK    K W+ G  ++ +V+V G  V  GSS QK IA+LNAAR+A  KL+ +  
Sbjct: 220 ELCQKHGKMAQFKTWQKGGMTVVNVFVAGELVGIGSSEQKVIAKLNAARDATRKLAGAKK 279

Query: 248 TRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKP 279
             + T V     DE  E+   KQKL++ C ++ W KP
Sbjct: 280 QVLTTGVGNGLGDEIGELRECKQKLNEQCSRQNWPKP 314

BLAST of Chy6G123590 vs. ExPASy Swiss-Prot
Match: Q9FKF0 (Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 3.2e-59
Identity = 141/384 (36.72%), Postives = 208/384 (54.17%), Query Frame = 0

Query: 3   SSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAYPH 62
           +SV AVE+IL+Y+F NK LL++A+T      SP ++RLEF GDS + +  TNY    YP+
Sbjct: 2   NSVEAVEKILNYSFVNKTLLKEAIT----QKSPLFDRLEFFGDSILEVAFTNYICHTYPN 61

Query: 63  LHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVPYG 122
           L    L  LR ANVS EK AR+AV H L++++    P++  KVK F ++V  E   VPYG
Sbjct: 62  LKVKELRDLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYG 121

Query: 123 GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQ-PVTVLF 182
           GL+KAPK+LAD +ES+A  V++DVN+D+++LW I R LLEPIYT +DL ++P+ P   LF
Sbjct: 122 GLVKAPKILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLF 181

Query: 183 QICQRNGKQ-----------------------VDIKHWKNGSKSIASVYVDGTFVASGSS 242
           ++  ++G +                       VD++  K  S  I S  +      S +S
Sbjct: 182 RLADKHGSESTSGIRKMTTSTKILLRNKDCLDVDLEDVKGKSFEICSTELFSLSTVSENS 241

Query: 243 MQKEIARLNAAREALIKLSDSMDTRIKTF---------------------------VTID 302
           +  E+++     +      +  D + K F                           + +D
Sbjct: 242 LTDEMSQEEVVIDEDSPNVEPEDVKGKLFEICYTRKLQIQTGSSGNPLTYEMTTKQMVVD 301

Query: 303 GVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVK--IATCYGTFYI 334
                 E    + +L + C K KW +P +SV+++ GP +E +FVCSVK  I    GTF++
Sbjct: 302 KDSLHVEPVNGRGELIEICTKNKWPRPIFSVQEEKGPKNEPKFVCSVKIEIPNIEGTFHM 361

BLAST of Chy6G123590 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 1.3e-49
Identity = 123/349 (35.24%), Postives = 192/349 (55.01%), Query Frame = 0

Query: 6    IAVERILSYNFKNKNLLEQALTHPSYSAS--PSYERLEFVGDSAIGLTITNYFFLAYPHL 65
            + +ER L Y FK K LL +A+TH S  +S    Y+RLEFVGD+ +   IT + F  Y  L
Sbjct: 1560 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1619

Query: 66   HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVPYG- 125
              G L+ LRAA V+ E  ARVAV H L+ Y+R  + A++ +++EFV  V  E S   +  
Sbjct: 1620 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1679

Query: 126  ---GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTV 185
               G  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1680 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1739

Query: 186  LFQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREAL--IKLS 245
            L + CQ+  + ++ K  ++G+ +   V++DG  V    + QK++A+  AAR AL  +K  
Sbjct: 1740 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1799

Query: 246  DSMDTRIKTFVTIDGVDESFEIEG----------AKQKLHDACCKRKWQKPNYSVEKDLG 305
            +  +++ K     +  ++  E E            +Q L+D C ++ W  P+Y   K+ G
Sbjct: 1800 EIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGG 1859

Query: 306  PSHERRFVCSVKIATC--YGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
            P+H +RF   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1860 PAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1906

BLAST of Chy6G123590 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 6.6e-49
Identity = 122/330 (36.97%), Postives = 188/330 (56.97%), Query Frame = 0

Query: 11   ILSYNFKNKNLLEQALTHPSYSAS--PSYERLEFVGDSAIGLTITNYFFLAYPHLHQGHL 70
            +L   F+NK LL +A+TH S  +S    Y+RLEFVGD+ +   IT + F  Y  L  G L
Sbjct: 1544 VLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTDLPPGRL 1603

Query: 71   SLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSV---ALEGSSVPYG-GL 130
            + LRAA V+ E  ARVAV H L+ ++R  + A++ +++EFV  V    L+     +G G 
Sbjct: 1604 TDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQEELLKPGFNSFGLGD 1663

Query: 131  MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVLFQIC 190
             KAPKVL DIVES+AGA+++D  +D   +W + + LL P+ T E L +   PV  L + C
Sbjct: 1664 CKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPETLPM--HPVRELQERC 1723

Query: 191  QRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSMDTRI 250
            Q+  + ++ K  + G+ +   V+VDG  +    + QK++A+  AAR AL+ L +  +T  
Sbjct: 1724 QQQAEGLEYKASRAGNIATVEVFVDGVQIGVAQNPQKKMAQKLAARNALVVLKEK-ETAT 1783

Query: 251  KTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKIATC- 310
            K     DG  ++      +Q L+D C +R+W  P Y    + GP+H +RFV SV++ T  
Sbjct: 1784 KKEDERDGEKKNGAQMFTRQTLNDICLRRQWPMPQYRCVNEGGPAHAKRFVYSVRVNTSD 1843

Query: 311  -YGTFYIVGDEKSRVKDAENSAASLMIRAL 333
               T   +G+    VK A++SAA L++  L
Sbjct: 1844 RGWTDECIGEPMPSVKKAKDSAAVLLLELL 1870

BLAST of Chy6G123590 vs. ExPASy TrEMBL
Match: A0A0A0L1E2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192230 PE=4 SV=1)

HSP 1 Score: 636.7 bits (1641), Expect = 5.4e-179
Identity = 319/338 (94.38%), Postives = 329/338 (97.34%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           MESSVIA+ERILSYNF NK+LLE+ALTHPSYSAS SYERLEFVGDSAIGL +TNYFFLA 
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFFLAS 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
           PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRD+PAMDDKVKEFVDSVALEGSSVP
Sbjct: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YGG+MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLE+LQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQ+CQRNGKQVDIKHWKNGS SIASV+VDG FVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVHVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           DTRIKTFVTIDG+DESFEIEGAKQKLHD C KRKWQKPNYSVEKDLGPSHER FVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 339
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of Chy6G123590 vs. ExPASy TrEMBL
Match: A0A5D3DKE4 (Ribonuclease 3-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00580 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.5e-168
Identity = 303/338 (89.64%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           M SSVIAVERILSY FKNK LLE+ALTH SY  S SYERLEF+GDSAIGL +TNYFFLAY
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
           P+LHQG LSLLRAANVSTEKLARVAVTHGLYNYVRR+A  MD KVKEFVDSVALEGSSVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLE+LQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F +CQRNGKQVDIK+WKNGSK+IASVYVDGTFVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           DT+IKT VTI+G+DESFEIEGAKQKLHD CC++KWQKP YS+EK LGPSH RRFVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 339
            TCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of Chy6G123590 vs. ExPASy TrEMBL
Match: A0A1S3BM12 (ribonuclease 3-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103491097 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.5e-168
Identity = 303/338 (89.64%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           M SSVIAVERILSY FKNK LLE+ALTH SY  S SYERLEF+GDSAIGL +TNYFFLAY
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
           P+LHQG LSLLRAANVSTEKLARVAVTHGLYNYVRR+A  MD KVKEFVDSVALEGSSVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLE+LQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F +CQRNGKQVDIK+WKNGSK+IASVYVDGTFVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           DT+IKT VTI+G+DESFEIEGAKQKLHD CC++KWQKP YS+EK LGPSH RRFVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 339
            TCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of Chy6G123590 vs. ExPASy TrEMBL
Match: A0A6J1HKS4 (ribonuclease 3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111465061 PE=4 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.7e-153
Identity = 271/338 (80.18%), Postives = 304/338 (89.94%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           MESSV+AVE I+SYNFKNK LLE+ALTH SY  S SY+RLEF+GDSAIGL  TNYFFLAY
Sbjct: 1   MESSVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
           P L QG LSLLRAAN+STEKLARVAV HGLY+YVRR A A+DDKV+EFVDSVALE +SVP
Sbjct: 61  PDLQQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGA+YVDVNFDLQ+LWVIIRGLLEPIYT EDL+VEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F++CQ+NGKQVDIK+W NGSK+++SV+VDGTFVASGSS  KEIARLNAAREAL KLS +M
Sbjct: 181 FEVCQKNGKQVDIKNWSNGSKTVSSVFVDGTFVASGSSTHKEIARLNAAREALAKLSKTM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           D  I+T VTIDG+DESFEIEGAKQKLHD CCK+KW KPNYS EKD GPSH++RF CSVKI
Sbjct: 241 DINIRTAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 339
           +T  G  Y+VGDEKSRVKD+ENSAAS+MIRALQE+ +L
Sbjct: 301 STSSGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

BLAST of Chy6G123590 vs. ExPASy TrEMBL
Match: A0A6J1F189 (ribonuclease 3-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111438633 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.5e-149
Identity = 265/338 (78.40%), Postives = 300/338 (88.76%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           MESSV+AVE I+SYNFKNK LLE+ALTH SY  S SY+RLEF+GDSAIGL  TNYFFLAY
Sbjct: 1   MESSVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
           P L QG LSLLRAAN+STEKLARVAV HGLY+YVRR A A+DDKV+EFVDSVALE +S+P
Sbjct: 61  PDLQQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSIP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGA+YVDVNFDLQ+LWVIIRGLLEPIYT EDL+VEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F++CQ+NGKQVDIK+W NGSK+++SV+VDG FVASGSS  K IARLNAAREAL K+S +M
Sbjct: 181 FEVCQKNGKQVDIKNWSNGSKTVSSVFVDGIFVASGSSTHKVIARLNAAREALAKMSKTM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           D  I+  VTIDG+DESFEIEGAKQKLHD CCK+KW KPNYS EKD GPSH++RF CSVKI
Sbjct: 241 DINIRAAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 339
           +   G  Y+VGDEKSRVKD+ENSAAS+MIRALQE+ +L
Sbjct: 301 SPPSGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

BLAST of Chy6G123590 vs. NCBI nr
Match: XP_031740595.1 (ribonuclease 3-like protein 2 [Cucumis sativus] >KAE8649376.1 hypothetical protein Csa_021577 [Cucumis sativus])

HSP 1 Score: 624 bits (1610), Expect = 1.07e-224
Identity = 319/338 (94.38%), Postives = 326/338 (96.45%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           MESSVIA+ERILSYNF NK+LLE+ALTHPSYSAS SYERLEFVGDSAIGL ITNYFFLA 
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAITNYFFLAS 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
           PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRD+PAMD KVKEFVDSVALEGSSV 
Sbjct: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDVKVKEFVDSVALEGSSVH 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YG LMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLE+LQVEPQPVTVL
Sbjct: 121 YGRLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQ+CQRNGKQVDIKHWKNGS SIASVYVDG FVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           DTRIKTFVTIDG+DESFEIEGAKQKLHD C KRKWQKPNYSVEKDLGPSHER FVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCSKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of Chy6G123590 vs. NCBI nr
Match: XP_031738803.1 (LOW QUALITY PROTEIN: ribonuclease 3-like protein 3 [Cucumis sativus])

HSP 1 Score: 629 bits (1622), Expect = 5.44e-221
Identity = 317/338 (93.79%), Postives = 327/338 (96.75%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           MESSVIA+ERILSYNF NK+LLE+ALTHPSYSAS SYERLEFVGDSAIGL +TNYF   +
Sbjct: 1   MESSVIALERILSYNFNNKSLLEEALTHPSYSASASYERLEFVGDSAIGLAVTNYFXSRF 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
            HLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRD+PAMDDKVKEFVDSVALEGSSVP
Sbjct: 61  SHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDSPAMDDKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YGG+MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLE+LQVEPQPVTVL
Sbjct: 121 YGGMMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           FQ+CQRNGKQVDIKHWKNGS SIASVYVDG FVASGSSMQKEIARLNAAREALIKLSDSM
Sbjct: 181 FQVCQRNGKQVDIKHWKNGSNSIASVYVDGKFVASGSSMQKEIARLNAAREALIKLSDSM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           DTRIKTFVTIDG+DESFEIEGAKQKLHD CCKRKWQKPNYSVEKDLGPSHER FVCSVKI
Sbjct: 241 DTRIKTFVTIDGIDESFEIEGAKQKLHDVCCKRKWQKPNYSVEKDLGPSHERIFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338
           ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of Chy6G123590 vs. NCBI nr
Match: XP_008449146.1 (PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo] >KAA0055862.1 ribonuclease 3-like protein 2 [Cucumis melo var. makuwa] >TYK24086.1 ribonuclease 3-like protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 596 bits (1536), Expect = 2.03e-213
Identity = 303/338 (89.64%), Postives = 318/338 (94.08%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           M SSVIAVERILSY FKNK LLE+ALTH SY  S SYERLEF+GDSAIGL +TNYFFLAY
Sbjct: 1   MGSSVIAVERILSYKFKNKKLLEEALTHCSYPDSASYERLEFIGDSAIGLALTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
           P+LHQG LSLLRAANVSTEKLARVAVTHGLYNYVRR+A  MD KVKEFVDSVALEGSSVP
Sbjct: 61  PNLHQGQLSLLRAANVSTEKLARVAVTHGLYNYVRRNAATMDAKVKEFVDSVALEGSSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLE+LQVEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEELQVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F +CQRNGKQVDIK+WKNGSK+IASVYVDGTFVASGSSMQKEIARLNAAREALIKLS SM
Sbjct: 181 FDVCQRNGKQVDIKNWKNGSKNIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSGSM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           DT+IKT VTI+G+DESFEIEGAKQKLHD CC++KWQKP YS+EK LGPSH RRFVCSVKI
Sbjct: 241 DTKIKTVVTINGIDESFEIEGAKQKLHDFCCRKKWQKPKYSIEKKLGPSHGRRFVCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338
            TCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL
Sbjct: 301 PTCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338

BLAST of Chy6G123590 vs. NCBI nr
Match: XP_022965096.1 (ribonuclease 3-like protein 2 [Cucurbita maxima])

HSP 1 Score: 545 bits (1403), Expect = 3.67e-193
Identity = 271/338 (80.18%), Postives = 304/338 (89.94%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           MESSV+AVE I+SYNFKNK LLE+ALTH SY  S SY+RLEF+GDSAIGL  TNYFFLAY
Sbjct: 1   MESSVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
           P L QG LSLLRAAN+STEKLARVAV HGLY+YVRR A A+DDKV+EFVDSVALE +SVP
Sbjct: 61  PDLQQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGA+YVDVNFDLQ+LWVIIRGLLEPIYT EDL+VEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F++CQ+NGKQVDIK+W NGSK+++SV+VDGTFVASGSS  KEIARLNAAREAL KLS +M
Sbjct: 181 FEVCQKNGKQVDIKNWSNGSKTVSSVFVDGTFVASGSSTHKEIARLNAAREALAKLSKTM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           D  I+T VTIDG+DESFEIEGAKQKLHD CCK+KW KPNYS EKD GPSH++RF CSVKI
Sbjct: 241 DINIRTAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338
           +T  G  Y+VGDEKSRVKD+ENSAAS+MIRALQE+ +L
Sbjct: 301 STSSGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

BLAST of Chy6G123590 vs. NCBI nr
Match: XP_023551903.1 (ribonuclease 3-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 542 bits (1397), Expect = 3.01e-192
Identity = 270/338 (79.88%), Postives = 303/338 (89.64%), Query Frame = 0

Query: 1   MESSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAY 60
           MESSV+AVE I+SYNFKNK LLE+ALTH SY  S SY+RLEF+GDSAIGL  TNYFFLAY
Sbjct: 1   MESSVLAVELIVSYNFKNKKLLEEALTHSSYVESASYQRLEFIGDSAIGLAFTNYFFLAY 60

Query: 61  PHLHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVP 120
           P L QG LSLLRAAN+STEKLARVAV HGLY+YVRR A A+DDKV+EFVDSVALE +SVP
Sbjct: 61  PDLQQGQLSLLRAANISTEKLARVAVRHGLYHYVRRKAVALDDKVREFVDSVALENNSVP 120

Query: 121 YGGLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVL 180
           YGGLMKAPKVLADIVESVAGA+YVDVNFDLQ+LWVIIRGLLEPIYT EDL+VEPQPVTVL
Sbjct: 121 YGGLMKAPKVLADIVESVAGAIYVDVNFDLQRLWVIIRGLLEPIYTPEDLEVEPQPVTVL 180

Query: 181 FQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSM 240
           F++CQ+NGKQVDIK+W NGSK+++SV+VDG FVASGSS  KEIARLNAAREAL KLS +M
Sbjct: 181 FEVCQKNGKQVDIKNWSNGSKTVSSVFVDGIFVASGSSTHKEIARLNAAREALAKLSKTM 240

Query: 241 DTRIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI 300
           D  I+T VTIDG+DESFEIEGAKQKLHD CCK+KW KPNYS EKD GPSH++RF CSVKI
Sbjct: 241 DINIRTAVTIDGIDESFEIEGAKQKLHDFCCKKKWPKPNYSTEKDFGPSHDKRFTCSVKI 300

Query: 301 ATCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 338
           +T  G  Y+VGDEKSRVKD+ENSAAS+MIRALQE+ +L
Sbjct: 301 STSSGMLYMVGDEKSRVKDSENSAASMMIRALQEKGYL 338

BLAST of Chy6G123590 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 371.7 bits (953), Expect = 6.3e-103
Identity = 193/337 (57.27%), Postives = 256/337 (75.96%), Query Frame = 0

Query: 4   SVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAYPHL 63
           S+ AVE+IL+Y F NK+LL++A+TH S +  PSYERLEF+GDSAIGL I+NY +L YP L
Sbjct: 59  SMEAVEKILNYKFSNKSLLKEAITHTSCTDFPSYERLEFIGDSAIGLAISNYLYLTYPSL 118

Query: 64  HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGS-SVPYG 123
               LSLLRAANVSTEKLARV++ HGLY+++RR+AP++D+KVKEF ++V  E   SV YG
Sbjct: 119 EPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVGKEDDLSVSYG 178

Query: 124 GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVLFQ 183
           GL+KAPKVLAD+ ES+AGAVYVDVNFDLQ+LWVI RGLLEPI TL+DLQ +PQPV++LF+
Sbjct: 179 GLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQKQPQPVSMLFK 238

Query: 184 ICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREALIKLSDSMDT 243
           +C ++ K++DIK+WK+G+ SIA +Y+D   +ASG +  K+IARL AA+EAL KLS+    
Sbjct: 239 LCHKHKKRIDIKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKEALRKLSEVFPV 298

Query: 244 RIKTFVTIDGVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVKI-A 303
                + ID      ++  AK KL++ C K+KW KP YSVE+D      +RFVCS KI  
Sbjct: 299 E----MVIDEDSVEIQLTHAKTKLNEICLKKKWPKPIYSVEEDRSSVQGKRFVCSAKIKI 358

Query: 304 TCYGTFYIVGDEKSRVKDAENSAASLMIRALQERKHL 339
           T   T Y+ GDE+S++K AE+S+A  MIRAL++  +L
Sbjct: 359 TEEKTLYMKGDEQSKIKKAESSSAYHMIRALRKSHYL 391

BLAST of Chy6G123590 vs. TAIR 10
Match: AT5G45150.1 (RNAse THREE-like protein 3 )

HSP 1 Score: 230.3 bits (586), Expect = 2.3e-60
Identity = 141/384 (36.72%), Postives = 208/384 (54.17%), Query Frame = 0

Query: 3   SSVIAVERILSYNFKNKNLLEQALTHPSYSASPSYERLEFVGDSAIGLTITNYFFLAYPH 62
           +SV AVE+IL+Y+F NK LL++A+T      SP ++RLEF GDS + +  TNY    YP+
Sbjct: 2   NSVEAVEKILNYSFVNKTLLKEAIT----QKSPLFDRLEFFGDSILEVAFTNYICHTYPN 61

Query: 63  LHQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVPYG 122
           L    L  LR ANVS EK AR+AV H L++++    P++  KVK F ++V  E   VPYG
Sbjct: 62  LKVKELRDLRTANVSNEKFARIAVNHNLHHFLLLQNPSLFKKVKNFAEAVRKEDDPVPYG 121

Query: 123 GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQ-PVTVLF 182
           GL+KAPK+LAD +ES+A  V++DVN+D+++LW I R LLEPIYT +DL ++P+ P   LF
Sbjct: 122 GLVKAPKILADTLESIAATVFIDVNYDVKRLWEIFRSLLEPIYTPDDLLLQPKLPFLTLF 181

Query: 183 QICQRNGKQ-----------------------VDIKHWKNGSKSIASVYVDGTFVASGSS 242
           ++  ++G +                       VD++  K  S  I S  +      S +S
Sbjct: 182 RLADKHGSESTSGIRKMTTSTKILLRNKDCLDVDLEDVKGKSFEICSTELFSLSTVSENS 241

Query: 243 MQKEIARLNAAREALIKLSDSMDTRIKTF---------------------------VTID 302
           +  E+++     +      +  D + K F                           + +D
Sbjct: 242 LTDEMSQEEVVIDEDSPNVEPEDVKGKLFEICYTRKLQIQTGSSGNPLTYEMTTKQMVVD 301

Query: 303 GVDESFEIEGAKQKLHDACCKRKWQKPNYSVEKDLGPSHERRFVCSVK--IATCYGTFYI 334
                 E    + +L + C K KW +P +SV+++ GP +E +FVCSVK  I    GTF++
Sbjct: 302 KDSLHVEPVNGRGELIEICTKNKWPRPIFSVQEEKGPKNEPKFVCSVKIEIPNIEGTFHM 361

BLAST of Chy6G123590 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 198.4 bits (503), Expect = 9.5e-51
Identity = 123/349 (35.24%), Postives = 192/349 (55.01%), Query Frame = 0

Query: 6    IAVERILSYNFKNKNLLEQALTHPSYSAS--PSYERLEFVGDSAIGLTITNYFFLAYPHL 65
            + +ER L Y FK K LL +A+TH S  +S    Y+RLEFVGD+ +   IT + F  Y  L
Sbjct: 1560 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1619

Query: 66   HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVPYG- 125
              G L+ LRAA V+ E  ARVAV H L+ Y+R  + A++ +++EFV  V  E S   +  
Sbjct: 1620 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1679

Query: 126  ---GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTV 185
               G  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1680 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1739

Query: 186  LFQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREAL--IKLS 245
            L + CQ+  + ++ K  ++G+ +   V++DG  V    + QK++A+  AAR AL  +K  
Sbjct: 1740 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1799

Query: 246  DSMDTRIKTFVTIDGVDESFEIEG----------AKQKLHDACCKRKWQKPNYSVEKDLG 305
            +  +++ K     +  ++  E E            +Q L+D C ++ W  P+Y   K+ G
Sbjct: 1800 EIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGG 1859

Query: 306  PSHERRFVCSVKIATC--YGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
            P+H +RF   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1860 PAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1906

BLAST of Chy6G123590 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 198.4 bits (503), Expect = 9.5e-51
Identity = 123/349 (35.24%), Postives = 192/349 (55.01%), Query Frame = 0

Query: 6    IAVERILSYNFKNKNLLEQALTHPSYSAS--PSYERLEFVGDSAIGLTITNYFFLAYPHL 65
            + +ER L Y FK K LL +A+TH S  +S    Y+RLEFVGD+ +   IT + F  Y  L
Sbjct: 1561 VGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLITRHLFFTYTSL 1620

Query: 66   HQGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVPYG- 125
              G L+ LRAA V+ E  ARVAV H L+ Y+R  + A++ +++EFV  V  E S   +  
Sbjct: 1621 PPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREFVKEVQTESSKPGFNS 1680

Query: 126  ---GLMKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTV 185
               G  KAPKVL DIVES+AGA+++D   D    W + + LL+P+ T E L +   PV  
Sbjct: 1681 FGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPMVTPETLPM--HPVRE 1740

Query: 186  LFQICQRNGKQVDIKHWKNGSKSIASVYVDGTFVASGSSMQKEIARLNAAREAL--IKLS 245
            L + CQ+  + ++ K  ++G+ +   V++DG  V    + QK++A+  AAR AL  +K  
Sbjct: 1741 LQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQKLAARNALAALKEK 1800

Query: 246  DSMDTRIKTFVTIDGVDESFEIEG----------AKQKLHDACCKRKWQKPNYSVEKDLG 305
            +  +++ K     +  ++  E E            +Q L+D C ++ W  P+Y   K+ G
Sbjct: 1801 EIAESKEKHINNGNAGEDQGENENGNKKNGHQPFTRQTLNDICLRKNWPMPSYRCVKEGG 1860

Query: 306  PSHERRFVCSVKIATC--YGTFYIVGDEKSRVKDAENSAASLMIRALQE 335
            P+H +RF   V++ T     T   +G+    VK A++SAA L++  L +
Sbjct: 1861 PAHAKRFTFGVRVNTSDRGWTDECIGEPMPSVKKAKDSAAVLLLELLNK 1907

BLAST of Chy6G123590 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 154.1 bits (388), Expect = 2.1e-37
Identity = 97/231 (41.99%), Postives = 141/231 (61.04%), Query Frame = 0

Query: 7   AVERILSYNFKNKNLLEQALTHPSY--SASPSYERLEFVGDSAIGLTITNYFFLAYPHLH 66
           ++E+IL+Y FK+K+LL +A T  SY    S SYE LE +GDS + + I   F   YP   
Sbjct: 31  SLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELLGDSILNMGIIYDFIKLYPKEA 90

Query: 67  QGHLSLLRAANVSTEKLARVAVTHGLYNYVRRDAPAMDDKVKEFVDSVALEGSSVPYGGL 126
            G L+ LRA NV TEKLARVAV H LY+Y+R   P +++++ EFV+  A+E   +   GL
Sbjct: 91  PGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQILEFVE--AMEKYPLHSNGL 150

Query: 127 MKAPKVLADIVESVAGAVYVDVNFDLQKLWVIIRGLLEPIYTLEDLQVEPQPVTVLFQIC 186
           +K PKVLADIVES  GA+++D N   + +W +I+ LLEPI  L+  +++  P+T L ++C
Sbjct: 151 LKVPKVLADIVESTIGAIFMDCN-STETVWKVIKPLLEPIIHLD--KMKSHPMTELNEMC 210

Query: 187 Q-RNGKQVDIKHWKNGSKSIASVYVDGTFVASG-SSMQKEIARLNAAREAL 234
           Q RN K      W+         +++  FV  G   ++KE AR  AA+ A+
Sbjct: 211 QKRNLKLTSKDTWE--ENQTYCFHIEDKFVGCGHHPVKKETARNFAAKNAI 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LTQ08.8e-10257.27Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1[more]
Q6ATG65.4e-6750.54Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0... [more]
Q9FKF03.2e-5936.72Ribonuclease 3-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=RTL3 PE=2 SV=1[more]
Q9SP321.3e-4935.24Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV... [more]
Q8LMR26.6e-4936.97Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL... [more]
Match NameE-valueIdentityDescription
A0A0A0L1E25.4e-17994.38Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G192230 PE=4 SV=1[more]
A0A5D3DKE42.5e-16889.64Ribonuclease 3-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BM122.5e-16889.64ribonuclease 3-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103491097 PE=4 SV=1[more]
A0A6J1HKS46.7e-15380.18ribonuclease 3-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111465061 PE=4 S... [more]
A0A6J1F1891.5e-14978.40ribonuclease 3-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111438633 PE=4... [more]
Match NameE-valueIdentityDescription
XP_031740595.11.07e-22494.38ribonuclease 3-like protein 2 [Cucumis sativus] >KAE8649376.1 hypothetical prote... [more]
XP_031738803.15.44e-22193.79LOW QUALITY PROTEIN: ribonuclease 3-like protein 3 [Cucumis sativus][more]
XP_008449146.12.03e-21389.64PREDICTED: ribonuclease 3-like protein 2 [Cucumis melo] >KAA0055862.1 ribonuclea... [more]
XP_022965096.13.67e-19380.18ribonuclease 3-like protein 2 [Cucurbita maxima][more]
XP_023551903.13.01e-19279.88ribonuclease 3-like protein 2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G20420.16.3e-10357.27RNAse THREE-like protein 2 [more]
AT5G45150.12.3e-6036.72RNAse THREE-like protein 3 [more]
AT1G01040.19.5e-5135.24dicer-like 1 [more]
AT1G01040.29.5e-5135.24dicer-like 1 [more]
AT4G15417.12.1e-3741.99RNAse II-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000999Ribonuclease III domainSMARTSM00535riboneu5coord: 20..170
e-value: 2.7E-34
score: 129.9
IPR000999Ribonuclease III domainPFAMPF00636Ribonuclease_3coord: 38..145
e-value: 7.8E-21
score: 74.7
IPR000999Ribonuclease III domainPROSITEPS00517RNASE_3_1coord: 38..46
IPR000999Ribonuclease III domainPROSITEPS50142RNASE_3_2coord: 5..147
score: 34.288834
IPR000999Ribonuclease III domainCDDcd00593RIBOccoord: 21..170
e-value: 2.75891E-41
score: 138.9
IPR014720Double-stranded RNA-binding domainSMARTSM00358DRBM_3coord: 176..237
e-value: 0.014
score: 22.7
coord: 262..333
e-value: 2.4E-9
score: 47.0
IPR014720Double-stranded RNA-binding domainPFAMPF00035dsrmcoord: 262..332
e-value: 1.2E-13
score: 51.6
coord: 176..236
e-value: 4.1E-6
score: 27.4
IPR014720Double-stranded RNA-binding domainPROSITEPS50137DS_RBDcoord: 261..334
score: 10.435972
IPR014720Double-stranded RNA-binding domainPROSITEPS50137DS_RBDcoord: 175..238
score: 9.804789
NoneNo IPR availableGENE3D3.30.160.20coord: 259..335
e-value: 2.5E-17
score: 64.9
NoneNo IPR availableGENE3D3.30.160.20coord: 172..245
e-value: 6.2E-8
score: 34.5
NoneNo IPR availablePANTHERPTHR14950:SF49RIBONUCLEASE 3-LIKE PROTEIN 2-RELATEDcoord: 7..329
NoneNo IPR availablePANTHERPTHR14950DICER-RELATEDcoord: 7..329
NoneNo IPR availableSUPERFAMILY54768dsRNA-binding domain-likecoord: 129..236
NoneNo IPR availableSUPERFAMILY54768dsRNA-binding domain-likecoord: 261..335
IPR036389Ribonuclease III, endonuclease domain superfamilyGENE3D1.10.1520.10Ribonuclease III domaincoord: 1..171
e-value: 1.8E-48
score: 166.7
IPR036389Ribonuclease III, endonuclease domain superfamilySUPERFAMILY69065RNase III domain-likecoord: 3..166

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G123590.1Chy6G123590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030422 production of siRNA involved in RNA interference
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process GO:0006396 RNA processing
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0004525 ribonuclease III activity
molecular_function GO:0003723 RNA binding