Chy6G120990 (gene) Cucumber (hystrix) v1

Overview


Name	Chy6G120990
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	alpha-1,2-Mannosidase
Location	chrH06: 17763095 .. 17763952 (+)
RNA-Seq Expression	Chy6G120990
Synteny	Chy6G120990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAGTTCTTCCGGTGAAACTTCCAATTCTGTTAACTATAGCGTGAGTTTTCATCCCTAAACTATACTTTTGATTATGAACTATATATTGTTGTGTGGCTTGTATCATTCTTCTTCCTCATTACTCTTCTTATTGAAATGAGCTAATCCATTATTTTTCTTTTGTCGAATGTGTAGTTTCTGCTATTCAGTCCTCAATAATTTTTGCATCATGTATTATTACGAAGAAAAGACATTGTAATAATGGATTTCCCAATTAAGTCACATCAAATAAGGAAGTTGGAAATAAATTCTTATTTGTTCTAATCGCTTAATACTAAACATGAACATTTTGATTGACTGAGTAGGAAGAAGCTTATATAGAATTTCTTTCCATAAGAAAGATTTCTATACAAAGTGAGAATTTATTGTAGTGATGCATCAAATATAGTTCCACAAATGAGGGTGTTGATTTTGGTGTTGAATGTTCTTAATTTTTTATTGGGTATGCATCATATTATGTTGAATGCATCGGATATAGCTCCAAAAGCCATAACTTTTCTCTTTTTTTTTTTTTTATTGTCTTCATTGAAGGAGGATAGGGGTATGAGATTTAGAATTAGTGTTTTCATGTTATTATGGATAACTTGGAAATATTGTCTGCTTTGAGTTTCATTTGCCTATGATTTGCCCCAATTTCTCTACTGTTCATCTATTTTCTAATTGTTTTAAGTAAGATGTAATTGTTATGTAGGCCATCTGTAAGATGTTTTATCATGCTTTCAATGGATACATGATGCATGCTTTTCCCTTAGATGAATTAAGACCTCTATTGTGTGAAGGAGAAGATTCACTTGGTGGTTATGCCTTGACTTTG

mRNA sequence

ATGTCGAGTTCTTCCGGTGAAACTTCCAATTCTGTTAACTATAGCGCCATCTGTAAGATGTTTTATCATGCTTTCAATGGATACATGATGCATGCTTTTCCCTTAGATGAATTAAGACCTCTATTGTGTGAAGGAGAAGATTCACTTGGTGGTTATGCCTTGACTTTG

Coding sequence (CDS)

ATGTCGAGTTCTTCCGGTGAAACTTCCAATTCTGTTAACTATAGCGCCATCTGTAAGATGTTTTATCATGCTTTCAATGGATACATGATGCATGCTTTTCCCTTAGATGAATTAAGACCTCTATTGTGTGAAGGAGAAGATTCACTTGGTGGTTATGCCTTGACTTTG

Protein sequence

MSSSSGETSNSVNYSAICKMFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL

Homology

[Close]

BLAST of Chy6G120990 vs. ExPASy Swiss-Prot
Match: Q9FG93 (Alpha-mannosidase I MNS4 OS=Arabidopsis thaliana OX=3702 GN=MNS4 PE=1 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 1.4e-12
Identity = 31/37 (83.78%), Postives = 35/37 (94.59%), Query Frame = 0


Query: 20 MFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 57
          MFYHAF+GYM +AFPLDELRPL C+GED+LGGYALTL
Sbjct: 47 MFYHAFDGYMNNAFPLDELRPLSCQGEDTLGGYALTL 83

[Close]

BLAST of Chy6G120990 vs. ExPASy Swiss-Prot
Match: Q9BV94 (ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Homo sapiens OX=9606 GN=EDEM2 PE=1 SV=2)

HSP 1 Score: 57.8 bits (138), Expect = 4.7e-08
Identity = 22/37 (59.46%), Postives = 30/37 (81.08%), Query Frame = 0


Query: 20 MFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 57
          MFYHA++ Y+ +AFP DELRPL C+G D+ G ++LTL
Sbjct: 42 MFYHAYDSYLENAFPFDELRPLTCDGHDTWGSFSLTL 78

[Close]

BLAST of Chy6G120990 vs. ExPASy Swiss-Prot
Match: Q8BJT9 (ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Mus musculus OX=10090 GN=Edem2 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 6.1e-08
Identity = 22/37 (59.46%), Postives = 30/37 (81.08%), Query Frame = 0


Query: 20 MFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 57
          MFYHA++ Y+ +AFP DELRPL C+G D+ G ++LTL
Sbjct: 42 MFYHAYDSYLENAFPYDELRPLTCDGHDTWGSFSLTL 78

[Close]

BLAST of Chy6G120990 vs. ExPASy Swiss-Prot
Match: Q6GQB9 (ER degradation-enhancing alpha-mannosidase-like protein 3 OS=Xenopus laevis OX=8355 GN=edem3 PE=2 SV=2)

HSP 1 Score: 49.3 bits (116), Expect = 1.7e-05
Identity = 23/54 (42.59%), Postives = 31/54 (57.41%), Query Frame = 0


Query: 15 SAICKMFYHAFNGYMMHAFPLDELRPLLCEG------------EDSLGGYALTL 57
          S + +MF HA+  YM HA+P DEL PL C G            +D+LG ++LTL
Sbjct: 40 SQVLEMFDHAYGNYMQHAYPADELMPLTCRGRIRGQEPSRGDVDDALGKFSLTL 93

[Close]

BLAST of Chy6G120990 vs. ExPASy Swiss-Prot
Match: D4AV26 (Probable mannosyl-oligosaccharide alpha-1,2-mannosidase ARB_00035 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_00035 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.7e-05
Identity = 21/40 (52.50%), Postives = 27/40 (67.50%), Query Frame = 0


Query: 16 AICKMFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALT 56
          AI   F HA+ GYM HA+P+DELRP+  EG + L G+  T
Sbjct: 43 AIKDAFNHAWKGYMTHAYPMDELRPVSNEGSNPLNGWGAT 82

[Close]

BLAST of Chy6G120990 vs. ExPASy TrEMBL
Match: A0A5A7U245 (alpha-1,2-Mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001010 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.5e-12
Identity = 35/38 (92.11%), Postives = 36/38 (94.74%), Query Frame = 0


Query: 19 KMFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 57
          +MFYHAFNGYM HAFPLDELRPL CEGEDSLGGYALTL
Sbjct: 47 EMFYHAFNGYMRHAFPLDELRPLSCEGEDSLGGYALTL 84

[Close]

BLAST of Chy6G120990 vs. ExPASy TrEMBL
Match: A0A6J1IG88 (alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111473105 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.5e-12
Identity = 35/38 (92.11%), Postives = 36/38 (94.74%), Query Frame = 0


Query: 19 KMFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 57
          +MFYHAFNGYM HAFPLDELRPL CEGEDSLGGYALTL
Sbjct: 47 EMFYHAFNGYMKHAFPLDELRPLSCEGEDSLGGYALTL 84

[Close]

BLAST of Chy6G120990 vs. ExPASy TrEMBL
Match: A0A0A0L5B0 (alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_3G143590 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.5e-12
Identity = 35/38 (92.11%), Postives = 36/38 (94.74%), Query Frame = 0


Query: 19 KMFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 57
          +MFYHAFNGYM HAFPLDELRPL CEGEDSLGGYALTL
Sbjct: 47 EMFYHAFNGYMKHAFPLDELRPLSCEGEDSLGGYALTL 84

[Close]

BLAST of Chy6G120990 vs. ExPASy TrEMBL
Match: A0A6J1CZW1 (alpha-1,2-Mannosidase OS=Momordica charantia OX=3673 GN=LOC111016308 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.5e-12
Identity = 35/38 (92.11%), Postives = 36/38 (94.74%), Query Frame = 0


Query: 19 KMFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 57
          +MFYHAFNGYM HAFPLDELRPL CEGEDSLGGYALTL
Sbjct: 47 EMFYHAFNGYMKHAFPLDELRPLSCEGEDSLGGYALTL 84

[Close]

BLAST of Chy6G120990 vs. ExPASy TrEMBL
Match: A0A6J1G9M4 (alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111452215 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 2.5e-12
Identity = 35/38 (92.11%), Postives = 36/38 (94.74%), Query Frame = 0


Query: 19 KMFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 57
          +MFYHAFNGYM HAFPLDELRPL CEGEDSLGGYALTL
Sbjct: 47 EMFYHAFNGYMKHAFPLDELRPLSCEGEDSLGGYALTL 84

[Close]

BLAST of Chy6G120990 vs. NCBI nr
Match: RYR38446.1 (hypothetical protein Ahy_A09g043489 isoform C [Arachis hypogaea])

HSP 1 Score: 79.0 bits (193), Expect = 1.90e-17
Identity = 32/40 (80.00%), Postives = 37/40 (92.50%), Query Frame = 0


Query: 17 ICKMFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 56
          +C+MFYHAFNGYM +AFPLDELRP+ C GED+LGGYALTL
Sbjct: 42 VCEMFYHAFNGYMDNAFPLDELRPMSCAGEDTLGGYALTL 81

[Close]

BLAST of Chy6G120990 vs. NCBI nr
Match: XP_023521335.1 (alpha-mannosidase I MNS4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 81.6 bits (200), Expect = 3.66e-17
Identity = 35/37 (94.59%), Postives = 35/37 (94.59%), Query Frame = 0


Query: 20 MFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 56
          MFYHAFNGYM HAFPLDELRPL CEGEDSLGGYALTL
Sbjct: 1  MFYHAFNGYMKHAFPLDELRPLSCEGEDSLGGYALTL 37

[Close]

BLAST of Chy6G120990 vs. NCBI nr
Match: KDO53923.1 (hypothetical protein CISIN_1g0475971mg, partial [Citrus sinensis])

HSP 1 Score: 79.7 bits (195), Expect = 6.61e-17
Identity = 34/38 (89.47%), Postives = 36/38 (94.74%), Query Frame = 0


Query: 19 KMFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 56
          +MFYHAF+GYM HAFPLDELRPL CEGEDSLGGYALTL
Sbjct: 20 EMFYHAFDGYMEHAFPLDELRPLSCEGEDSLGGYALTL 57

[Close]

BLAST of Chy6G120990 vs. NCBI nr
Match: VAH80449.1 (unnamed protein product [Triticum turgidum subsp. durum])

HSP 1 Score: 79.0 bits (193), Expect = 1.13e-16
Identity = 33/37 (89.19%), Postives = 35/37 (94.59%), Query Frame = 0


Query: 20 MFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 56
          MFYHAF+GYM HAFPLDELRPL C+GEDSLGGYALTL
Sbjct: 1  MFYHAFDGYMQHAFPLDELRPLSCQGEDSLGGYALTL 37

[Close]

BLAST of Chy6G120990 vs. NCBI nr
Match: VAH64601.1 (unnamed protein product [Triticum turgidum subsp. durum])

HSP 1 Score: 79.0 bits (193), Expect = 1.13e-16
Identity = 33/37 (89.19%), Postives = 35/37 (94.59%), Query Frame = 0


Query: 20 MFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 56
          MFYHAF+GYM HAFPLDELRPL C+GEDSLGGYALTL
Sbjct: 1  MFYHAFDGYMQHAFPLDELRPLSCQGEDSLGGYALTL 37

[Close]

BLAST of Chy6G120990 vs. TAIR 10
Match: AT5G43710.1 (Glycosyl hydrolase family 47 protein )

HSP 1 Score: 72.8 bits (177), Expect = 9.9e-14
Identity = 31/37 (83.78%), Postives = 35/37 (94.59%), Query Frame = 0


Query: 20 MFYHAFNGYMMHAFPLDELRPLLCEGEDSLGGYALTL 57
          MFYHAF+GYM +AFPLDELRPL C+GED+LGGYALTL
Sbjct: 47 MFYHAFDGYMNNAFPLDELRPLSCQGEDTLGGYALTL 83

[Close]

BLAST of Chy6G120990 vs. TAIR 10
Match: AT1G27520.1 (Glycosyl hydrolase family 47 protein )

HSP 1 Score: 41.2 bits (95), Expect = 3.2e-04
Identity = 21/48 (43.75%), Postives = 27/48 (56.25%), Query Frame = 0


Query: 2  SSSSGETSNSVNYSAICKMFYHAFNGYMMHAFPLDELRPLLCEGEDSL 50
          SS   E         + +MFYHA++ YM +AFP DEL+PL     DSL
Sbjct: 26 SSQHIEVKKKQMREKVREMFYHAYDNYMTYAFPHDELKPLTKSFTDSL 73

The following BLAST results are available for this feature:

BLAST of Chy6G120990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FG93	1.4e-12	83.78	Alpha-mannosidase I MNS4 OS=Arabidopsis thaliana OX=3702 GN=MNS4 PE=1 SV=1	[more]
Q9BV94	4.7e-08	59.46	ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Homo sapiens OX=960...	[more]
Q8BJT9	6.1e-08	59.46	ER degradation-enhancing alpha-mannosidase-like protein 2 OS=Mus musculus OX=100...	[more]
Q6GQB9	1.7e-05	42.59	ER degradation-enhancing alpha-mannosidase-like protein 3 OS=Xenopus laevis OX=8...	[more]
D4AV26	1.7e-05	52.50	Probable mannosyl-oligosaccharide alpha-1,2-mannosidase ARB_00035 OS=Arthroderma...	[more]

BLAST of Chy6G120990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U245	2.5e-12	92.11	alpha-1,2-Mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold43...	[more]
A0A6J1IG88	2.5e-12	92.11	alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111473105 PE=3 SV=1	[more]
A0A0A0L5B0	2.5e-12	92.11	alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_3G143590 PE=3 SV=1	[more]
A0A6J1CZW1	2.5e-12	92.11	alpha-1,2-Mannosidase OS=Momordica charantia OX=3673 GN=LOC111016308 PE=3 SV=1	[more]
A0A6J1G9M4	2.5e-12	92.11	alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111452215 PE=3 SV=1	[more]

BLAST of Chy6G120990 vs. NCBI nr


Match Name	E-value	Identity	Description
RYR38446.1	1.90e-17	80.00	hypothetical protein Ahy_A09g043489 isoform C [Arachis hypogaea]	[more]
XP_023521335.1	3.66e-17	94.59	alpha-mannosidase I MNS4-like [Cucurbita pepo subsp. pepo]	[more]
KDO53923.1	6.61e-17	89.47	hypothetical protein CISIN_1g0475971mg, partial [Citrus sinensis]	[more]
VAH80449.1	1.13e-16	89.19	unnamed protein product [Triticum turgidum subsp. durum]	[more]
VAH64601.1	1.13e-16	89.19	unnamed protein product [Triticum turgidum subsp. durum]	[more]

BLAST of Chy6G120990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43710.1	9.9e-14	83.78	Glycosyl hydrolase family 47 protein	[more]
AT1G27520.1	3.2e-04	43.75	Glycosyl hydrolase family 47 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012341	Six-hairpin glycosidase-like superfamily	GENE3D	1.50.10.10		coord: 2..56 e-value: 6.4E-12 score: 46.8
IPR001382	Glycoside hydrolase family 47	PFAM	PF01532	Glyco_hydro_47	coord: 20..56 e-value: 7.3E-8 score: 31.7
None	No IPR available	PANTHER	PTHR45679:SF6	ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2	coord: 16..56
IPR044674	ER degradation-enhancing alpha-mannosidase-like protein 1/2/3	PANTHER	PTHR45679	ER DEGRADATION-ENHANCING ALPHA-MANNOSIDASE-LIKE PROTEIN 2	coord: 16..56
IPR036026	Seven-hairpin glycosidases	SUPERFAMILY	48225	Seven-hairpin glycosidases	coord: 15..56

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy6G120990.1	Chy6G120990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:1904380	endoplasmic reticulum mannose trimming
biological_process	GO:1904382	mannose trimming involved in glycoprotein ERAD pathway
cellular_component	GO:0044322	endoplasmic reticulum quality control compartment
cellular_component	GO:0016020	membrane
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0004571	mannosyl-oligosaccharide 1,2-alpha-mannosidase activity