Chy6G115070 (gene) Cucumber (hystrix) v1

Overview
NameChy6G115070
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionSterol regulatory element-binding protein cleavage-activating protein, putative
LocationchrH06: 7241101 .. 7241578 (-)
RNA-Seq ExpressionChy6G115070
SyntenyChy6G115070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCAAAGAACCCTAATAATCAACGACTCAACAATCACGACAACATCCAGAGGCCAAGTCCAAGTAAACGCCAAAGGCTTCACTAATTCTCCAGCGACGGCGAAAAACAGAGAAACCGCCGCGAAAAACCTCTCCGCCCAAAACAACAACGAAGACGACGACGACGAAATTCCAGAAGCCGTATACAGCAGGATTATAACGAGAATCTTGGCATTCGTCGGAATTCCGATGGCGTTCGGTGTGACGTTGTTGAAGATTTTCCAAGCGATTAAGGAGCAAAACCTTTGGGATGTGCCTATTTGGGTGCCATTTTTTACGACTTTCTTGACATTCGGAGCTTCAACGATGGGGATTGCTTACGGGACGTTATCGACGAGTTTGGATCCGGAGAAGAAAGGATCTTTGTTGGGATGGGCAGAGGCGCAGAAGAATTGGGTTGAAATGTGGAAGGAGGAAGATGAAGCCCGGCGCTGA

mRNA sequence

ATGGAATCAAAGAACCCTAATAATCAACGACTCAACAATCACGACAACATCCAGAGGCCAAGTCCAAAAGCCGTATACAGCAGGATTATAACGAGAATCTTGGCATTCGTCGGAATTCCGATGGCGTTCGGTGTGACGTTGTTGAAGATTTTCCAAGCGATTAAGGAGCAAAACCTTTGGGATGTGCCTATTTGGGTGCCATTTTTTACGACTTTCTTGACATTCGGAGCTTCAACGATGGGGATTGCTTACGGGACGTTATCGACGAGTTTGGATCCGGAGAAGAAAGGATCTTTGTTGGGATGGGCAGAGGCGCAGAAGAATTGGGTTGAAATGTGGAAGGAGGAAGATGAAGCCCGGCGCTGA

Coding sequence (CDS)

ATGGAATCAAAGAACCCTAATAATCAACGACTCAACAATCACGACAACATCCAGAGGCCAAGTCCAAAAGCCGTATACAGCAGGATTATAACGAGAATCTTGGCATTCGTCGGAATTCCGATGGCGTTCGGTGTGACGTTGTTGAAGATTTTCCAAGCGATTAAGGAGCAAAACCTTTGGGATGTGCCTATTTGGGTGCCATTTTTTACGACTTTCTTGACATTCGGAGCTTCAACGATGGGGATTGCTTACGGGACGTTATCGACGAGTTTGGATCCGGAGAAGAAAGGATCTTTGTTGGGATGGGCAGAGGCGCAGAAGAATTGGGTTGAAATGTGGAAGGAGGAAGATGAAGCCCGGCGCTGA

Protein sequence

MESKNPNNQRLNNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR*
Homology
BLAST of Chy6G115070 vs. ExPASy Swiss-Prot
Match: Q9LTD9 (Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 2.3e-28
Identity = 53/97 (54.64%), Postives = 76/97 (78.35%), Query Frame = 0

Query: 20  PSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTTFLTFGAST 79
           P P+ V+ R++ RI+  VG P+  GV +LK+ + +K++N+WDVP+WVP+ TT +TFG+S 
Sbjct: 71  PIPQEVFERMMGRIVVSVGTPLGLGVAILKVLEVLKDRNVWDVPLWVPYLTTLVTFGSSA 130

Query: 80  MGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEE 117
           +GIAYG+LST+LDP K  SL G  EA++NWVEMWKE+
Sbjct: 131 LGIAYGSLSTNLDPAKTNSLFGLKEAKENWVEMWKED 167

BLAST of Chy6G115070 vs. ExPASy Swiss-Prot
Match: O49668 (Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 1.6e-16
Identity = 40/93 (43.01%), Postives = 60/93 (64.52%), Query Frame = 0

Query: 22  PKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTTFLTFGASTMG 81
           P+ V +R+I+R+   VG+P+  G+     F  +K     DVP WVPF  +F+ FG +  G
Sbjct: 113 PEIVTNRMISRMGFTVGLPLFIGLLFFPFFYYLKVGLKVDVPTWVPFIVSFVFFGTALAG 172

Query: 82  IAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWK 115
           ++YG +S+S DP ++GSLLGW EA+KNW   W+
Sbjct: 173 VSYGIVSSSWDPLREGSLLGWNEAKKNWPVFWQ 205

BLAST of Chy6G115070 vs. ExPASy TrEMBL
Match: A0A0A0LLM4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G213970 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.7e-48
Identity = 100/119 (84.03%), Postives = 106/119 (89.08%), Query Frame = 0

Query: 3   SKNPNNQRLNNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 62
           +KN      NN+++     P+AVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV
Sbjct: 62  AKNRETTAQNNNEDDDDEIPEAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 121

Query: 63  PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 122
           PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGS+LGW EAQKNWVEMWKEEDE RR
Sbjct: 122 PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSVLGWEEAQKNWVEMWKEEDEGRR 180

BLAST of Chy6G115070 vs. ExPASy TrEMBL
Match: A0A5A7STB5 (Putative Sterol regulatory element-binding protein cleavage-activating protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001970 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 6.8e-47
Identity = 99/119 (83.19%), Postives = 107/119 (89.92%), Query Frame = 0

Query: 3   SKNPNNQRLNNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 62
           +KN + Q  NN+++     P+AVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV
Sbjct: 68  AKNLSGQ--NNNEDDDDEIPEAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 127

Query: 63  PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 122
           PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGS++GW EAQKNWVEMWKEEDE  R
Sbjct: 128 PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSVMGWEEAQKNWVEMWKEEDEVSR 184

BLAST of Chy6G115070 vs. ExPASy TrEMBL
Match: A0A1S3AZ57 (uncharacterized protein PAM68-like OS=Cucumis melo OX=3656 GN=LOC103484134 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 6.8e-47
Identity = 99/119 (83.19%), Postives = 107/119 (89.92%), Query Frame = 0

Query: 3   SKNPNNQRLNNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 62
           +KN + Q  NN+++     P+AVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV
Sbjct: 68  AKNLSGQ--NNNEDDDDEIPEAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 127

Query: 63  PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 122
           PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGS++GW EAQKNWVEMWKEEDE  R
Sbjct: 128 PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSVMGWEEAQKNWVEMWKEEDEVSR 184

BLAST of Chy6G115070 vs. ExPASy TrEMBL
Match: A0A5D3DHC5 (Putative Sterol regulatory element-binding protein cleavage-activating protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G001260 PE=4 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 8.8e-47
Identity = 99/119 (83.19%), Postives = 107/119 (89.92%), Query Frame = 0

Query: 3   SKNPNNQRLNNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 62
           +KN + Q  NN+++     P+AVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV
Sbjct: 68  AKNLSGQ--NNNEDDDDEIPEAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 127

Query: 63  PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 122
           PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGS++GW EAQKNWVEMWKEEDE  R
Sbjct: 128 PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSVVGWEEAQKNWVEMWKEEDEVSR 184

BLAST of Chy6G115070 vs. ExPASy TrEMBL
Match: A0A6J1CGP3 (uncharacterized protein PAM68-like OS=Momordica charantia OX=3673 GN=LOC111011500 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 8.0e-40
Identity = 86/115 (74.78%), Postives = 96/115 (83.48%), Query Frame = 0

Query: 7   NNQRLNNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWV 66
           NN   ++ D I     +AV +RIITRILAFVG+PMAFGV LLKIF+ IKEQ +WDVPIW+
Sbjct: 74  NNSSNDDDDEIS----EAVSNRIITRILAFVGVPMAFGVALLKIFETIKEQKVWDVPIWL 133

Query: 67  PFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 122
           PF TTFLTFGAST+GIAYGTLS SLDPEK+GSL GW EAQKNWVEMWKEEDEA R
Sbjct: 134 PFLTTFLTFGASTIGIAYGTLSASLDPEKEGSLFGWEEAQKNWVEMWKEEDEASR 184

BLAST of Chy6G115070 vs. NCBI nr
Match: XP_004150568.1 (uncharacterized protein PAM68-like [Cucumis sativus] >KGN61652.1 hypothetical protein Csa_006286 [Cucumis sativus])

HSP 1 Score: 201 bits (510), Expect = 2.35e-63
Identity = 100/119 (84.03%), Postives = 106/119 (89.08%), Query Frame = 0

Query: 3   SKNPNNQRLNNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 62
           +KN      NN+++     P+AVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV
Sbjct: 62  AKNRETTAQNNNEDDDDEIPEAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDV 121

Query: 63  PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 121
           PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGS+LGW EAQKNWVEMWKEEDE RR
Sbjct: 122 PIWVPFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSVLGWEEAQKNWVEMWKEEDEGRR 180

BLAST of Chy6G115070 vs. NCBI nr
Match: XP_008439311.1 (PREDICTED: uncharacterized protein PAM68-like [Cucumis melo] >KAA0033723.1 putative Sterol regulatory element-binding protein cleavage-activating protein [Cucumis melo var. makuwa])

HSP 1 Score: 197 bits (500), Expect = 8.87e-62
Identity = 96/110 (87.27%), Postives = 102/110 (92.73%), Query Frame = 0

Query: 12  NNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTT 71
           NN+++     P+AVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTT
Sbjct: 75  NNNEDDDDEIPEAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTT 134

Query: 72  FLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 121
           FLTFGASTMGIAYGTLSTSLDPEKKGS++GW EAQKNWVEMWKEEDE  R
Sbjct: 135 FLTFGASTMGIAYGTLSTSLDPEKKGSVMGWEEAQKNWVEMWKEEDEVSR 184

BLAST of Chy6G115070 vs. NCBI nr
Match: TYK23016.1 (putative Sterol regulatory element-binding protein cleavage-activating protein [Cucumis melo var. makuwa])

HSP 1 Score: 196 bits (499), Expect = 1.26e-61
Identity = 96/110 (87.27%), Postives = 102/110 (92.73%), Query Frame = 0

Query: 12  NNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTT 71
           NN+++     P+AVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTT
Sbjct: 75  NNNEDDDDEIPEAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTT 134

Query: 72  FLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 121
           FLTFGASTMGIAYGTLSTSLDPEKKGS++GW EAQKNWVEMWKEEDE  R
Sbjct: 135 FLTFGASTMGIAYGTLSTSLDPEKKGSVVGWEEAQKNWVEMWKEEDEVSR 184

BLAST of Chy6G115070 vs. NCBI nr
Match: XP_038877436.1 (uncharacterized protein PAM68-like [Benincasa hispida])

HSP 1 Score: 190 bits (482), Expect = 5.01e-59
Identity = 94/115 (81.74%), Postives = 103/115 (89.57%), Query Frame = 0

Query: 7   NNQRLNNHDNIQRPSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWV 66
           NN++ ++ D I    P+AVYSRIITRILAFVG+PMAFGVTLLKIFQAIKEQN+WDVPIW+
Sbjct: 74  NNKKEDDDDEI----PEAVYSRIITRILAFVGVPMAFGVTLLKIFQAIKEQNVWDVPIWL 133

Query: 67  PFFTTFLTFGASTMGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 121
           PF TTFLTFGASTMGIAYGTLSTSLDPEKKGS+ GW EAQKNWVEMWKEEDE  R
Sbjct: 134 PFLTTFLTFGASTMGIAYGTLSTSLDPEKKGSVFGWEEAQKNWVEMWKEEDEEAR 184

BLAST of Chy6G115070 vs. NCBI nr
Match: XP_022140980.1 (uncharacterized protein PAM68-like [Momordica charantia])

HSP 1 Score: 172 bits (437), Expect = 3.37e-52
Identity = 82/99 (82.83%), Postives = 90/99 (90.91%), Query Frame = 0

Query: 23  KAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTTFLTFGASTMGI 82
           +AV +RIITRILAFVG+PMAFGV LLKIF+ IKEQ +WDVPIW+PF TTFLTFGAST+GI
Sbjct: 86  EAVSNRIITRILAFVGVPMAFGVALLKIFETIKEQKVWDVPIWLPFLTTFLTFGASTIGI 145

Query: 83  AYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEEDEARR 121
           AYGTLS SLDPEK+GSL GW EAQKNWVEMWKEEDEA R
Sbjct: 146 AYGTLSASLDPEKEGSLFGWEEAQKNWVEMWKEEDEASR 184

BLAST of Chy6G115070 vs. TAIR 10
Match: AT5G52780.1 (Protein of unknown function (DUF3464) )

HSP 1 Score: 126.3 bits (316), Expect = 1.7e-29
Identity = 53/97 (54.64%), Postives = 76/97 (78.35%), Query Frame = 0

Query: 20  PSPKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTTFLTFGAST 79
           P P+ V+ R++ RI+  VG P+  GV +LK+ + +K++N+WDVP+WVP+ TT +TFG+S 
Sbjct: 71  PIPQEVFERMMGRIVVSVGTPLGLGVAILKVLEVLKDRNVWDVPLWVPYLTTLVTFGSSA 130

Query: 80  MGIAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWKEE 117
           +GIAYG+LST+LDP K  SL G  EA++NWVEMWKE+
Sbjct: 131 LGIAYGSLSTNLDPAKTNSLFGLKEAKENWVEMWKED 167

BLAST of Chy6G115070 vs. TAIR 10
Match: AT4G19100.1 (Protein of unknown function (DUF3464) )

HSP 1 Score: 87.0 bits (214), Expect = 1.1e-17
Identity = 40/93 (43.01%), Postives = 60/93 (64.52%), Query Frame = 0

Query: 22  PKAVYSRIITRILAFVGIPMAFGVTLLKIFQAIKEQNLWDVPIWVPFFTTFLTFGASTMG 81
           P+ V +R+I+R+   VG+P+  G+     F  +K     DVP WVPF  +F+ FG +  G
Sbjct: 113 PEIVTNRMISRMGFTVGLPLFIGLLFFPFFYYLKVGLKVDVPTWVPFIVSFVFFGTALAG 172

Query: 82  IAYGTLSTSLDPEKKGSLLGWAEAQKNWVEMWK 115
           ++YG +S+S DP ++GSLLGW EA+KNW   W+
Sbjct: 173 VSYGIVSSSWDPLREGSLLGWNEAKKNWPVFWQ 205

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LTD92.3e-2854.64Uncharacterized protein PAM68-like OS=Arabidopsis thaliana OX=3702 GN=At5g52780 ... [more]
O496681.6e-1643.01Protein PAM68, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PAM68 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LLM42.7e-4884.03Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G213970 PE=4 SV=1[more]
A0A5A7STB56.8e-4783.19Putative Sterol regulatory element-binding protein cleavage-activating protein O... [more]
A0A1S3AZ576.8e-4783.19uncharacterized protein PAM68-like OS=Cucumis melo OX=3656 GN=LOC103484134 PE=4 ... [more]
A0A5D3DHC58.8e-4783.19Putative Sterol regulatory element-binding protein cleavage-activating protein O... [more]
A0A6J1CGP38.0e-4074.78uncharacterized protein PAM68-like OS=Momordica charantia OX=3673 GN=LOC11101150... [more]
Match NameE-valueIdentityDescription
XP_004150568.12.35e-6384.03uncharacterized protein PAM68-like [Cucumis sativus] >KGN61652.1 hypothetical pr... [more]
XP_008439311.18.87e-6287.27PREDICTED: uncharacterized protein PAM68-like [Cucumis melo] >KAA0033723.1 putat... [more]
TYK23016.11.26e-6187.27putative Sterol regulatory element-binding protein cleavage-activating protein [... [more]
XP_038877436.15.01e-5981.74uncharacterized protein PAM68-like [Benincasa hispida][more]
XP_022140980.13.37e-5282.83uncharacterized protein PAM68-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G52780.11.7e-2954.64Protein of unknown function (DUF3464) [more]
AT4G19100.11.1e-1743.01Protein of unknown function (DUF3464) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021855PAM68-likePFAMPF11947DUF3464coord: 7..115
e-value: 5.2E-37
score: 126.9
IPR021855PAM68-likePANTHERPTHR34575PROTEIN PAM68, CHLOROPLASTICcoord: 7..119
NoneNo IPR availablePANTHERPTHR34575:SF6EXPRESSED PROTEINcoord: 7..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy6G115070.1Chy6G115070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane