Chy6G114790 (gene) Cucumber (hystrix) v1

Overview


Name	Chy6G114790
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Cytochrome P450
Location	chrH06: 6983859 .. 6986465 (+)
RNA-Seq Expression	Chy6G114790
Synteny	Chy6G114790

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGGGAAGAAGTTTGTATATTGGTTGGGGACAGAGCCATTTTTGTACATAGCAGATCCACAGCTTGTGAAGAAGATATCTGAAGCTGTGTTGGGTAAGAGTTGGGGGAAGCCAGCAGTGTTCAGAAACGACCGAGAACCGATGTTCGGCGATGGCCTTGTCATGACTGAGGGAGACAACTGGATTCGCCATCGCCATATCCTCACTCCCGCCTTCATCCCTGCCAATTTGAAGGTAATTTGACTTGCATGTTTCACTCCTAATTTAAGTTAATTGTGTAACGAAAGATTATATTATTTTTAGTTCAACGATATTTGAAAAAGATTCGTCATACGTTTGATCTTTTGAGTTATGTTATTGTCATGGCAACTACTTTTATAAAATATCTAACCTAATTAAGCTTTCAAGTATTACAATATGACATTCTGTTTAGTCTTTTAAATTCAAACTTGTTCAAAGTTATATATGGTAAAATGATTATGAATCTTCCAACAAATTAGGTAAAAGAAATATTAATATTAGTATTAATATTAATATTATTTAGAAAAAACAAATTTAATTTGTTCATTAATATATTGGTGGTGGAGATATGACAGATTCTATATGAACCAATATTTATAAGGACCGAATAAATGAGATGATTAGTTGATGGTTTGGTTGACAACATTGTAATATTTGTCTCTTTTATGGTAATAAAAAAATTAAATATTAAATAACGAATTTTGTTTTTCTTTTTTCAAATTTATTTTTTAATACTCATTATTCTTACCTTTCTAGTTTAATTTTCATTGGTTTGTAGTTTCTAAAATATTAGACATAAGTTAAATTAACTTTTCTTTTAAGTTTCAAACGTTATCGTATCTGTTTTAATTTTTTTTAAGTTTCAATATTTTATGTTTTCATTCTCAATTTTCGTTAAAGTACAATTTATAATTTTATTTTTAGTGTTAACTTCGAATTGATTAATTTGAAATATTATAATCTAATAAATTTTAATTAATTTTAATCGTGATAAAAAATTAGTAAAAATTAATTATAATCAACAACAAAGTTGATATTTCTTGAAATAAAACTAAAATATAAGTAACTTATTTTCCTAGCATGTCTCTTAGTAACTAACATAATGAAGTAAAATATTAATTTTATTAATATAATTTAAAATTTTGTTATTTTTTTAATACATATTTTCAATAATTTTGTAATATATATGTATATATATATATAGTTATTTTTAAATGCAAATAATAATGTGAATTTTTTTTTATTTTTTTTTTTAAATTTCAGGCAATGACAAAATTCATGGTGGAGTCGACGACAAAAATGCTAGAAGAATGGAGAAGCCTAGTAAAATCAGGGCAGTCAGAAATAGAGGTGGAAAGAGAAATAACGGCCACTGCGGGTGAAATAATCGCAAAAGCAAGCTTTGGAATATCGAAATGCGGCGGCGGCGGAGAAGCATTGAACAACCTGAGAGCTCTACAGTTAACTCTTTTTAAGAACAGTAGCTACGTGGGGGTCCCATTCAGCAGCCTAGTTCTTTACCCAGCACGAACCCTAGCCGCCAAGCGACTGGGTGCTCAAATCGACCGCCTATTTTTCTCCATCATATCTGATAGGAAAATTTCGGGTTCGGGTTCGGCATACTCGGATTTGCTGAGCAGGTTGATTGGGAGTGAGTATAAGGGCGGCGCGGGGCTGAGTCCGAGAGAAGTGGTGGATGAGTGTAAGACATTTTTCTTTGGGGGACATGAGACCACAGCGCTGGCTATTAGTTGGACGTTGCTGTTGTTGGCCACGTCTCCCGAATGGCAAACCATTTTGCGAAATGAGATTAAAGAAGTGGTTGGTGATAAAGATATTGACTTCTCCATGCTTTCTTCCCTCAAAAAGGTTCCCTTTTCTATCTTTCTTTTTAACTTACACTGTAAATTCTATTTACTTAATATTCTCCTATTTTCTTTCATTTTCTTTTTGGAGTCATATTACTATATTTTGCAAGTATTTTCAACACCTTTCTCATTTACTGTAAATTTCTTTTTCTTTTTAAGTATCTAATTATAATTAAAAATTAAACCTTATTTATTTAAATTAAGCATATTTCTTTAATTAACCATGCATGCATACATTTTAACCAGGAAACATGAATATTTATTTACTATGTGGATGCAGATGGGATGGGTATGGAACGAGGTACTTAGACTATACCCCTCAGCTCCAAACATACAAAGACAAGCAAAAGGAGACATCGATTTAGGGGACGTAAAAATCCCAAAGGGGACCAACATATGGATAGACATTGTTGCAATGCATCACGACCCCACCCTATGGGGCGACGATGTTAACGAGTTCAATCCAAAGAGGTTCCAACACGACACCATTCATGGCGGATGCAACCACAAAATGGGATATTTGCCTTTTGGCTTTGGAGGGAGAATGTGCATTGGCAGAAACTTATCTTCTATGGAGTATAAGATTGTCCTAACCCTAATTCTTTCTACCTTTTCACTCTCACTTTCTCCTTCTTACTCCCATTCTCCTGCTACTTTACTCTCTCTGAGGCCAGCCCATGGCATTCCTCTCATTCTAACCCCTCTTCACCCTTAA

mRNA sequence

ATGACAGGGAAGAAGTTTGTATATTGGTTGGGGACAGAGCCATTTTTGTACATAGCAGATCCACAGCTTGTGAAGAAGATATCTGAAGCTGTGTTGGGTAAGAGTTGGGGGAAGCCAGCAGTGTTCAGAAACGACCGAGAACCGATGTTCGGCGATGGCCTTGTCATGACTGAGGGAGACAACTGGATTCGCCATCGCCATATCCTCACTCCCGCCTTCATCCCTGCCAATTTGAAGGCAATGACAAAATTCATGGTGGAGTCGACGACAAAAATGCTAGAAGAATGGAGAAGCCTAGTAAAATCAGGGCAGTCAGAAATAGAGGTGGAAAGAGAAATAACGGCCACTGCGGGTGAAATAATCGCAAAAGCAAGCTTTGGAATATCGAAATGCGGCGGCGGCGGAGAAGCATTGAACAACCTGAGAGCTCTACAGTTAACTCTTTTTAAGAACAGTAGCTACGTGGGGGTCCCATTCAGCAGCCTAGTTCTTTACCCAGCACGAACCCTAGCCGCCAAGCGACTGGGTGCTCAAATCGACCGCCTATTTTTCTCCATCATATCTGATAGGAAAATTTCGGGTTCGGGTTCGGCATACTCGGATTTGCTGAGCAGGTTGATTGGGAGTGAGTATAAGGGCGGCGCGGGGCTGAGTCCGAGAGAAGTGGTGGATGAGTGTAAGACATTTTTCTTTGGGGGACATGAGACCACAGCGCTGGCTATTAGTTGGACGTTGCTGTTGTTGGCCACGTCTCCCGAATGGCAAACCATTTTGCGAAATGAGATTAAAGAAGTGGTTGGTGATAAAGATATTGACTTCTCCATGCTTTCTTCCCTCAAAAAGATGGGATGGGTATGGAACGAGGTACTTAGACTATACCCCTCAGCTCCAAACATACAAAGACAAGCAAAAGGAGACATCGATTTAGGGGACGTAAAAATCCCAAAGGGGACCAACATATGGATAGACATTGTTGCAATGCATCACGACCCCACCCTATGGGGCGACGATGTTAACGAGTTCAATCCAAAGAGGTTCCAACACGACACCATTCATGGCGGATGCAACCACAAAATGGGATATTTGCCTTTTGGCTTTGGAGGGAGAATGTGCATTGGCAGAAACTTATCTTCTATGGAGTATAAGATTGTCCTAACCCTAATTCTTTCTACCTTTTCACTCTCACTTTCTCCTTCTTACTCCCATTCTCCTGCTACTTTACTCTCTCTGAGGCCAGCCCATGGCATTCCTCTCATTCTAACCCCTCTTCACCCTTAA

Coding sequence (CDS)

ATGACAGGGAAGAAGTTTGTATATTGGTTGGGGACAGAGCCATTTTTGTACATAGCAGATCCACAGCTTGTGAAGAAGATATCTGAAGCTGTGTTGGGTAAGAGTTGGGGGAAGCCAGCAGTGTTCAGAAACGACCGAGAACCGATGTTCGGCGATGGCCTTGTCATGACTGAGGGAGACAACTGGATTCGCCATCGCCATATCCTCACTCCCGCCTTCATCCCTGCCAATTTGAAGGCAATGACAAAATTCATGGTGGAGTCGACGACAAAAATGCTAGAAGAATGGAGAAGCCTAGTAAAATCAGGGCAGTCAGAAATAGAGGTGGAAAGAGAAATAACGGCCACTGCGGGTGAAATAATCGCAAAAGCAAGCTTTGGAATATCGAAATGCGGCGGCGGCGGAGAAGCATTGAACAACCTGAGAGCTCTACAGTTAACTCTTTTTAAGAACAGTAGCTACGTGGGGGTCCCATTCAGCAGCCTAGTTCTTTACCCAGCACGAACCCTAGCCGCCAAGCGACTGGGTGCTCAAATCGACCGCCTATTTTTCTCCATCATATCTGATAGGAAAATTTCGGGTTCGGGTTCGGCATACTCGGATTTGCTGAGCAGGTTGATTGGGAGTGAGTATAAGGGCGGCGCGGGGCTGAGTCCGAGAGAAGTGGTGGATGAGTGTAAGACATTTTTCTTTGGGGGACATGAGACCACAGCGCTGGCTATTAGTTGGACGTTGCTGTTGTTGGCCACGTCTCCCGAATGGCAAACCATTTTGCGAAATGAGATTAAAGAAGTGGTTGGTGATAAAGATATTGACTTCTCCATGCTTTCTTCCCTCAAAAAGATGGGATGGGTATGGAACGAGGTACTTAGACTATACCCCTCAGCTCCAAACATACAAAGACAAGCAAAAGGAGACATCGATTTAGGGGACGTAAAAATCCCAAAGGGGACCAACATATGGATAGACATTGTTGCAATGCATCACGACCCCACCCTATGGGGCGACGATGTTAACGAGTTCAATCCAAAGAGGTTCCAACACGACACCATTCATGGCGGATGCAACCACAAAATGGGATATTTGCCTTTTGGCTTTGGAGGGAGAATGTGCATTGGCAGAAACTTATCTTCTATGGAGTATAAGATTGTCCTAACCCTAATTCTTTCTACCTTTTCACTCTCACTTTCTCCTTCTTACTCCCATTCTCCTGCTACTTTACTCTCTCTGAGGCCAGCCCATGGCATTCCTCTCATTCTAACCCCTCTTCACCCTTAA

Protein sequence

MTGKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNWIRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRLFFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAISWTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTPLHP*

Homology

[Close]

BLAST of Chy6G114790 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 288.1 bits (736), Expect = 1.6e-76
Identity = 166/429 (38.69%), Postives = 241/429 (56.18%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKI---SEAVLGKSWGKPAVFRNDREPMFGDGLVMTEG 62
           GK+F+ W GTEP L + + +++K++      V GKSW    + +   +   G GL+M  G
Sbjct: 95  GKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSW----LQQQGTKGFIGRGLLMANG 154

Query: 63  DNWIRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGE 122
           + W   RH+  PAF    LK   K MVE T  M E  R   K    E+E+  E+     +
Sbjct: 155 EAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLR---KEVGEEVEIGEEMRRLTAD 214

Query: 123 IIAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQI 182
           II++  FG S C  G E  + L  LQ    + + ++  P  S  L        K L  ++
Sbjct: 215 IISRTEFG-SSCDKGKELFSLLTVLQRLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEV 274

Query: 183 DRLFFSIISDRKIS---GSGSAY-SDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHE 242
           +RL   II  RK S   G  S+Y  DLL  L+         L+ + ++DECKTFFF GHE
Sbjct: 275 ERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKNNLNVQMIMDECKTFFFTGHE 334

Query: 243 TTALAISWTLLLLATSPEWQTILRNEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLYP 302
           TT+L ++WTL+LLA +P WQ  +R+E+++V G   +     LSSL  +  V NE LRLYP
Sbjct: 335 TTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYP 394

Query: 303 SAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGG 362
            A  + R A  DI LGD+ IPKG +IWI ++A+HH   LWG+D NEFNP+RF   +    
Sbjct: 395 PATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-AS 454

Query: 363 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAH 422
             H   ++PF  G R CIG+  + ME KI+L +++S FS ++S +Y H+P  +L+++P +
Sbjct: 455 SRH---FMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKY 510

Query: 423 GIPLILTPL 424
           G+ L+L PL
Sbjct: 515 GVQLVLKPL 510

[Close]

BLAST of Chy6G114790 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 9.8e-74
Identity = 160/435 (36.78%), Postives = 240/435 (55.17%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKI---SEAVLGKSWGKPAVFRNDREPMFGDGLVMTEG 62
           GK+F+ W GT+P L + + +L+K++      V G+SW +    +N      G GL+M  G
Sbjct: 94  GKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRSWLQQQGTKN----FIGRGLLMANG 153

Query: 63  DNWIRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGE 122
            +W   RH+  PAF    LK   + MVE T+K++E  R  V  G +E+E+  E+     +
Sbjct: 154 QDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTAD 213

Query: 123 IIAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQI 182
           II++  FG S    G E  N+L  LQ    + + ++  P  S  L        K L  ++
Sbjct: 214 IISRTKFG-SSFEKGKELFNHLTVLQRRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEV 273

Query: 183 DRLFFSIISDRK---ISGSGSAYSD-----LLSRLIGSEYKGGAGLSPREVVDECKTFFF 242
           +RL   II  R+     G  S + D     LL+ +   +       + + ++DECKTFFF
Sbjct: 274 ERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKTFFF 333

Query: 243 GGHETTALAISWTLLLLATSPEWQTILRNEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVL 302
            GHETTAL ++WT +LLA +P WQ  +R E++EV G   +     LS L  +  V NE L
Sbjct: 334 AGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESL 393

Query: 303 RLYPSAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDT 362
           RLYP A  + R A  D+ LGD+ IPKG +IWI ++A+HH   LWG D N+FNP+RF    
Sbjct: 394 RLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERF---- 453

Query: 363 IHGGCNHKMG--YLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLL 422
             GG     G  ++PF  G R CIG+  + ME KI+L  ++S F+ ++S +Y H+P  +L
Sbjct: 454 --GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVL 513

Query: 423 SLRPAHGIPLILTPL 424
           +++P +G+ +IL PL
Sbjct: 514 TIKPKYGVQVILKPL 516

[Close]

BLAST of Chy6G114790 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.2e-73
Identity = 156/426 (36.62%), Postives = 233/426 (54.69%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           G  ++Y  G+   L + DP +VK+++        GKP   + +R  + G G++ + GD W
Sbjct: 102 GSIYLYSTGSIQVLNVTDPNMVKELANC-KSLDLGKPCYLQKERGALLGMGILTSNGDLW 161

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLV--KSGQSEIEVEREITATAGEI 122
           +  R ++ P      +K M   M+E+   ML  W++ V  + G +EI V+  +   + ++
Sbjct: 162 VHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSWKNEVEDRGGSAEIVVDEFLRTFSADV 221

Query: 123 IAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPART-LAAKRLGAQI 182
           I++A FG S    G E    +R LQ  + K S  +GVP S  +  P R+      L + I
Sbjct: 222 ISRACFG-SSFSEGKEIFIKIRQLQKAMAKQSMLIGVPGSRYL--PTRSNRGIWNLDSSI 281

Query: 183 DRLFFSIISDRKISGSGSAYSDLLSRLI-GSEYKGGAGLSPRE-VVDECKTFFFGGHETT 242
             L  +I    +   S S   DLL  +I GS+    A  +P + +VD CK  +F GHETT
Sbjct: 282 RTLILNISKKYEHDSSTSVNKDLLHSIIQGSKDGPFASCTPEDFIVDNCKNIYFAGHETT 341

Query: 243 ALAISWTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAP 302
           +   +W L+LLA+  EWQ+  R E  ++   + +DF +L  LKK+  V  E LRLYP A 
Sbjct: 342 STTAAWCLMLLASHHEWQSRARVESLDICQGRPLDFDILRKLKKLTMVIQETLRLYPPAS 401

Query: 303 NIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNH 362
            + R+A  D+ LG + IPKGTNIWI I   H DP++WG   ++F+P RF +  I G C  
Sbjct: 402 FVAREALNDMKLGGIDIPKGTNIWIPIAMAHRDPSVWGPSADKFDPDRFANG-IAGACKP 461

Query: 363 KMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIP 422
              Y+PFG G R C G+NL+ +E K+VL+L+LS F   LSP+Y H PA  L++ P  G+P
Sbjct: 462 PHMYMPFGVGVRTCAGQNLAMVELKVVLSLLLSKFEFKLSPNYVHCPAFRLTIEPGKGVP 521

Query: 423 LILTPL 424
           LI   L
Sbjct: 522 LIFREL 522

[Close]

BLAST of Chy6G114790 vs. ExPASy Swiss-Prot
Match: B6SSW8 (Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 3.6e-68
Identity = 154/426 (36.15%), Postives = 228/426 (53.52%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           G  F Y +G   FL+++ P +V+ I+  V     GK +  +   EP+FG G++ + G+ W
Sbjct: 106 GPIFTYSMGNVVFLHVSRPDVVRDINLCV-SLDLGKSSYLKATHEPLFGGGILKSNGEAW 165

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLV--KSGQSEIEVEREITATAGEI 122
           +  R I+ P F    +K M   MV+S   +L  W   V    G ++I+++ +I A + ++
Sbjct: 166 LHQRKIIAPEFFLDKVKGMVDLMVDSAQPLLMSWEERVDRNGGITDIKIDDDIRAYSADV 225

Query: 123 IAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPA-RTLAAKRLGAQI 182
           I++  FG S    G E    +R LQ  + K +  V    + L  +P+ R   A  L  Q+
Sbjct: 226 ISRTCFGSSYI-KGKEIFMKIRELQQAVSKPN--VLAEMTGLRFFPSMRNKQAWELHKQV 285

Query: 183 DRLFFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPRE--VVDECKTFFFGGHETT 242
            +L   I+   K SG      +LLS ++ S      G++  E  +VD CK+ +F GHE+T
Sbjct: 286 RKLILEIV---KESGED---RNLLSAILHSASTSRVGIAEAENFIVDNCKSIYFAGHEST 345

Query: 243 ALAISWTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAP 302
           A+  +W L+LL   PEWQ  +R E+ EV   + +D   L  +K +  V  E LRLYP+  
Sbjct: 346 AVTAAWCLMLLGLHPEWQNRVREEVHEVCRGQPVDSRSLQKMKNLTMVIQETLRLYPAGA 405

Query: 303 NIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQ--HDTIHGGC 362
            + RQA  ++ LG V IPKG NI+I +  MH DP LWG DV EFNP+RF      +H   
Sbjct: 406 FVSRQALQELKLGGVHIPKGVNIYIPVSTMHLDPELWGPDVKEFNPERFSDVRPQLH--- 465

Query: 363 NHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHG 422
                YLPFG G R C+G+  +  E KI+++LI+S F L LSP Y HSP   L + P  G
Sbjct: 466 ----SYLPFGAGARTCLGQGFAMAELKILISLIVSKFVLKLSPHYQHSPTLKLIVEPELG 514

[Close]

BLAST of Chy6G114790 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 8.0e-68
Identity = 166/433 (38.34%), Postives = 234/433 (54.04%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLG---KSWGKPAVFRNDREPMFGDGLVMTEG 62
           G+ F+YW GTEP + I+DP+L K +    LG   KS  +P   +     +   GLV  EG
Sbjct: 93  GETFLYWNGTEPRICISDPELAKTMLSNKLGFFVKSKARPEAVK----LVGSKGLVFIEG 152

Query: 63  DNWIRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSE-----IEVEREIT 122
            +W+RHR IL PAF    LK MT  MV+ T KMLEEWR      ++E      E+  E  
Sbjct: 153 ADWVRHRRILNPAFSIDRLKIMTTVMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQ 212

Query: 123 ATAGEIIAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKR 182
               +IIA ++FG S    G E   +   L+     + + V +P +  +  P+  +   +
Sbjct: 213 RLTADIIATSAFG-SSYVEGIEVFRSQMELKRCYTTSLNQVSIPGTQYLPTPS-NIRVWK 272

Query: 183 LGAQIDRLFFSIISDRKISGSGSAYSDLLSRLIGSEYKGG--AGLSPREVVDECKTFFFG 242
           L  ++D     IIS R  S S     DLL  L+ +    G    +S  E++ EC+TFFFG
Sbjct: 273 LERKMDNSIKRIISSRLQSKSDYG-DDLLGILLKAYNTEGKERKMSIEEIIHECRTFFFG 332

Query: 243 GHETTALAISWTLLLLATSPEWQTILRNEI-KEVVGDKDIDFSMLSSLKKMGWVWNEVLR 302
           GHETT+  ++WT +LL+   +WQ  LR EI KE   +K  D    S LK M  V  E LR
Sbjct: 333 GHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLR 392

Query: 303 LYPSAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTI 362
           LY     + R+A  +I LGD++IPKGT + I ++ MH D TLWG D ++FNP RF +  +
Sbjct: 393 LYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANG-V 452

Query: 363 HGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFS-LSLSPSYSHSPATLLSL 422
               NH    L F  G R CIG+N   +E K VLT+IL  F  +SL   Y H+P   +++
Sbjct: 453 SRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTI 512

Query: 423 RPAHGIPLILTPL 424
           +P +G+P++L PL
Sbjct: 513 QPQYGLPVMLQPL 517

[Close]

BLAST of Chy6G114790 vs. ExPASy TrEMBL
Match: A0A5D3DI76 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold386G00640 PE=3 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 1.8e-235
Identity = 403/423 (95.27%), Postives = 410/423 (96.93%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW
Sbjct: 43  GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 102

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           IRHRHILTPAF P NLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVER+ITATAGEIIA
Sbjct: 103 IRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIA 162

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGIS CGGGGEALNNLRALQLTLFKNSSYVGVPFSSL LYPARTLAAKRLGAQIDRL
Sbjct: 163 KASFGISNCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRL 222

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 242
           F SIIS+RK+SGSGS YSDLLSRLIGSEY GG  LSPREVVDECKTFFFGGHETTALAIS
Sbjct: 223 FLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAIS 282

Query: 243 WTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 302
           WTLLLLATSP+WQ ILR EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ
Sbjct: 283 WTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 342

Query: 303 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 362
           AKGDIDLGD+KIPKGTNIWID+VAMHHDPTLWGDDVN+FNP RFQHDTIHGGCNHKMGYL
Sbjct: 343 AKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYL 402

Query: 363 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 422
           PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP
Sbjct: 403 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 462

Query: 423 LHP 426
           L P
Sbjct: 463 LQP 465

[Close]

BLAST of Chy6G114790 vs. ExPASy TrEMBL
Match: A0A5A7SWW2 (Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold239G001260 PE=3 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.5e-234
Identity = 402/423 (95.04%), Postives = 409/423 (96.69%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW
Sbjct: 43  GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 102

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           IRHRHILTPAF P NLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVER+ITATAGEIIA
Sbjct: 103 IRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIA 162

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGIS CGGGGEALNNLRALQLTLFKNSSYVGVPFSSL LYPARTLAAKRLGAQIDRL
Sbjct: 163 KASFGISNCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRL 222

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 242
           F SIIS+RK+SGSGS YSDLLSRLIGSEY GG  LSPREVVDECKTFFFGGHETTALAIS
Sbjct: 223 FLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAIS 282

Query: 243 WTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 302
           WTLLLLATSP+WQ ILR EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ
Sbjct: 283 WTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 342

Query: 303 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 362
           AKGDIDLGD+KIPKGTNIWID+VAMHHDPTLWGDDVN+FNP RFQHDTIHGGC HKMGYL
Sbjct: 343 AKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYL 402

Query: 363 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 422
           PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP
Sbjct: 403 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 462

Query: 423 LHP 426
           L P
Sbjct: 463 LQP 465

[Close]

BLAST of Chy6G114790 vs. ExPASy TrEMBL
Match: A0A1S3AXX2 (cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 1.5e-234
Identity = 402/423 (95.04%), Postives = 409/423 (96.69%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW
Sbjct: 103 GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 162

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           IRHRHILTPAF P NLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVER+ITATAGEIIA
Sbjct: 163 IRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIA 222

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGIS CGGGGEALNNLRALQLTLFKNSSYVGVPFSSL LYPARTLAAKRLGAQIDRL
Sbjct: 223 KASFGISNCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRL 282

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 242
           F SIIS+RK+SGSGS YSDLLSRLIGSEY GG  LSPREVVDECKTFFFGGHETTALAIS
Sbjct: 283 FLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAIS 342

Query: 243 WTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 302
           WTLLLLATSP+WQ ILR EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ
Sbjct: 343 WTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 402

Query: 303 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 362
           AKGDIDLGD+KIPKGTNIWID+VAMHHDPTLWGDDVN+FNP RFQHDTIHGGC HKMGYL
Sbjct: 403 AKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYL 462

Query: 363 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 422
           PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP
Sbjct: 463 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 522

Query: 423 LHP 426
           L P
Sbjct: 523 LQP 525

[Close]

BLAST of Chy6G114790 vs. ExPASy TrEMBL
Match: A0A6J1CIQ8 (cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1)

HSP 1 Score: 687.6 bits (1773), Expect = 3.4e-194
Identity = 340/429 (79.25%), Postives = 374/429 (87.18%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIA+PQ VK +SEAV+GK WGKPAVFRNDREPMFGDGLVMTEGD+W
Sbjct: 108 GKKFVYWLGTEPFLYIAEPQFVKNMSEAVMGKGWGKPAVFRNDREPMFGDGLVMTEGDDW 167

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           IRHRHILTPAF PANLKAMT+ MV STTKML+EW   + SG  E++VEREITATAG+IIA
Sbjct: 168 IRHRHILTPAFNPANLKAMTRLMVGSTTKMLDEWARRLSSGGPEMDVEREITATAGDIIA 227

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGI     G +A N LR LQ TLF+NSSYVGVPFSS+ LYP RTL AKRLG++IDRL
Sbjct: 228 KASFGICD-RSGRDAFNKLRDLQFTLFRNSSYVGVPFSSIFLYPTRTLTAKRLGSEIDRL 287

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYK------GGAGLSPREVVDECKTFFFGGHET 242
           F SIIS+RK S   SA SDLLSRLI S+        G + L+ RE+VDECKTFFFGGHET
Sbjct: 288 FLSIISERKQSPDASA-SDLLSRLIESQRNDSAPAAGKSWLTTRELVDECKTFFFGGHET 347

Query: 243 TALAISWTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSA 302
           TALAISWT+LLLAT PEWQTILR EIKEV+GD++IDF+MLS LKKMGWV+NEVLRLYPSA
Sbjct: 348 TALAISWTMLLLATRPEWQTILREEIKEVIGDREIDFAMLSRLKKMGWVFNEVLRLYPSA 407

Query: 303 PNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCN 362
           PNIQRQAKGDI +GD  IPKGTNIWIDIVAMHHDP+LWGDDVNEF P+RF+ D+IHGGC 
Sbjct: 408 PNIQRQAKGDIKVGDTTIPKGTNIWIDIVAMHHDPSLWGDDVNEFKPERFEKDSIHGGCK 467

Query: 363 HKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGI 422
           HKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLI+S FSLS+SPSYSHSPA LLSLRPAHG+
Sbjct: 468 HKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLIISRFSLSISPSYSHSPAILLSLRPAHGL 527

Query: 423 PLILTPLHP 426
           PLIL PLHP
Sbjct: 528 PLILKPLHP 534

[Close]

BLAST of Chy6G114790 vs. ExPASy TrEMBL
Match: A0A6J1HYD5 (cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.7e-190
Identity = 336/427 (78.69%), Postives = 369/427 (86.42%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIADP  +K+IS AV+ K WGKPAVFRNDR PMFGDGLVMTEGD W
Sbjct: 100 GKKFVYWLGTEPFLYIADPHFMKQISVAVMAKGWGKPAVFRNDRRPMFGDGLVMTEGDVW 159

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           +RHRHILTPAF PANLKAMTKFMVESTTKML EW SLV S   EI+VEREITATAG+IIA
Sbjct: 160 VRHRHILTPAFNPANLKAMTKFMVESTTKMLNEWSSLVNSASGEIDVEREITATAGDIIA 219

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGI   GGG +A + LRALQ TLF+NS+YVGVPFSSL   P RTLAAKRLGA IDRL
Sbjct: 220 KASFGICN-GGGRDAFDYLRALQFTLFQNSTYVGVPFSSLFFCPGRTLAAKRLGAAIDRL 279

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYK----GGAGLSPREVVDECKTFFFGGHETTA 242
           F S++S+RK+S + S  SDLLSRLI  +      G  GL+ RE+VDECKTFFFGGHETTA
Sbjct: 280 FLSLVSERKLSQNSS--SDLLSRLIEGQKSTGDVGKMGLTTRELVDECKTFFFGGHETTA 339

Query: 243 LAISWTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPN 302
           LA SWTLLLLAT PEWQ ILR EIKEV+GDK+IDF+MLS LKKMG V++EVLRLYPSAPN
Sbjct: 340 LATSWTLLLLATRPEWQDILREEIKEVIGDKEIDFNMLSGLKKMGCVFSEVLRLYPSAPN 399

Query: 303 IQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHK 362
           IQRQAKGDI++G + IPKGTNIWIDIVAMH DP LWGDD NEF P+RF  D+I GGCNHK
Sbjct: 400 IQRQAKGDIEVGSMTIPKGTNIWIDIVAMHRDPDLWGDDANEFKPERFVEDSIRGGCNHK 459

Query: 363 MGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPL 422
           MGYLPFGFGGRMCIG++LSSMEYKIVLTLILS FSLSLSP+YSHSPATLLSLRPAHG+PL
Sbjct: 460 MGYLPFGFGGRMCIGKSLSSMEYKIVLTLILSRFSLSLSPNYSHSPATLLSLRPAHGLPL 519

Query: 423 ILTPLHP 426
           I+TPLHP
Sbjct: 520 IVTPLHP 523

[Close]

BLAST of Chy6G114790 vs. NCBI nr
Match: KGN57414.2 (hypothetical protein Csa_010508 [Cucumis sativus])

HSP 1 Score: 843 bits (2178), Expect = 5.43e-307
Identity = 415/423 (98.11%), Postives = 419/423 (99.05%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW
Sbjct: 84  GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 143

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           IRHRHILTPAF PANLKAMTKFMVEST KMLEEWR  VKSGQSEIEVEREITATAGEIIA
Sbjct: 144 IRHRHILTPAFNPANLKAMTKFMVESTKKMLEEWRRQVKSGQSEIEVEREITATAGEIIA 203

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL
Sbjct: 204 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 263

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 242
           FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS
Sbjct: 264 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 323

Query: 243 WTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 302
           WTLLLLATSP+WQTILR+EIKEV+GDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ
Sbjct: 324 WTLLLLATSPDWQTILRDEIKEVIGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 383

Query: 303 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 362
           AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL
Sbjct: 384 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 443

Query: 363 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 422
           PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSY+HSPATLLSLRPAHGIPLILTP
Sbjct: 444 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYTHSPATLLSLRPAHGIPLILTP 503

Query: 423 LHP 425
           LHP
Sbjct: 504 LHP 506

[Close]

BLAST of Chy6G114790 vs. NCBI nr
Match: XP_004151435.2 (cytokinin hydroxylase [Cucumis sativus])

HSP 1 Score: 843 bits (2178), Expect = 1.11e-306
Identity = 415/423 (98.11%), Postives = 419/423 (99.05%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW
Sbjct: 103 GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 162

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           IRHRHILTPAF PANLKAMTKFMVEST KMLEEWR  VKSGQSEIEVEREITATAGEIIA
Sbjct: 163 IRHRHILTPAFNPANLKAMTKFMVESTKKMLEEWRRQVKSGQSEIEVEREITATAGEIIA 222

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL
Sbjct: 223 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 282

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 242
           FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS
Sbjct: 283 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 342

Query: 243 WTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 302
           WTLLLLATSP+WQTILR+EIKEV+GDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ
Sbjct: 343 WTLLLLATSPDWQTILRDEIKEVIGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 402

Query: 303 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 362
           AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL
Sbjct: 403 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 462

Query: 363 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 422
           PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSY+HSPATLLSLRPAHGIPLILTP
Sbjct: 463 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYTHSPATLLSLRPAHGIPLILTP 522

Query: 423 LHP 425
           LHP
Sbjct: 523 LHP 525

[Close]

BLAST of Chy6G114790 vs. NCBI nr
Match: TYK22959.1 (cytokinin hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 822 bits (2122), Expect = 3.96e-299
Identity = 403/423 (95.27%), Postives = 410/423 (96.93%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW
Sbjct: 43  GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 102

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           IRHRHILTPAF P NLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVER+ITATAGEIIA
Sbjct: 103 IRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIA 162

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGIS CGGGGEALNNLRALQLTLFKNSSYVGVPFSSL LYPARTLAAKRLGAQIDRL
Sbjct: 163 KASFGISNCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRL 222

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 242
           F SIIS+RK+SGSGS YSDLLSRLIGSEY GG  LSPREVVDECKTFFFGGHETTALAIS
Sbjct: 223 FLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAIS 282

Query: 243 WTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 302
           WTLLLLATSP+WQ ILR EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ
Sbjct: 283 WTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 342

Query: 303 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 362
           AKGDIDLGD+KIPKGTNIWID+VAMHHDPTLWGDDVN+FNP RFQHDTIHGGCNHKMGYL
Sbjct: 343 AKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCNHKMGYL 402

Query: 363 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 422
           PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP
Sbjct: 403 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 462

Query: 423 LHP 425
           L P
Sbjct: 463 LQP 465

[Close]

BLAST of Chy6G114790 vs. NCBI nr
Match: KAA0033659.1 (cytokinin hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 818 bits (2114), Expect = 6.55e-298
Identity = 402/423 (95.04%), Postives = 409/423 (96.69%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW
Sbjct: 43  GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 102

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           IRHRHILTPAF P NLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVER+ITATAGEIIA
Sbjct: 103 IRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIA 162

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGIS CGGGGEALNNLRALQLTLFKNSSYVGVPFSSL LYPARTLAAKRLGAQIDRL
Sbjct: 163 KASFGISNCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRL 222

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 242
           F SIIS+RK+SGSGS YSDLLSRLIGSEY GG  LSPREVVDECKTFFFGGHETTALAIS
Sbjct: 223 FLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAIS 282

Query: 243 WTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 302
           WTLLLLATSP+WQ ILR EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ
Sbjct: 283 WTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 342

Query: 303 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 362
           AKGDIDLGD+KIPKGTNIWID+VAMHHDPTLWGDDVN+FNP RFQHDTIHGGC HKMGYL
Sbjct: 343 AKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYL 402

Query: 363 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 422
           PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP
Sbjct: 403 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 462

Query: 423 LHP 425
           L P
Sbjct: 463 LQP 465

[Close]

BLAST of Chy6G114790 vs. NCBI nr
Match: XP_008439236.1 (PREDICTED: cytokinin hydroxylase [Cucumis melo])

HSP 1 Score: 818 bits (2114), Expect = 6.20e-297
Identity = 402/423 (95.04%), Postives = 409/423 (96.69%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW
Sbjct: 103 GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 162

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
           IRHRHILTPAF P NLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVER+ITATAGEIIA
Sbjct: 163 IRHRHILTPAFNPTNLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVERQITATAGEIIA 222

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           KASFGIS CGGGGEALNNLRALQLTLFKNSSYVGVPFSSL LYPARTLAAKRLGAQIDRL
Sbjct: 223 KASFGISNCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLFLYPARTLAAKRLGAQIDRL 282

Query: 183 FFSIISDRKISGSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTALAIS 242
           F SIIS+RK+SGSGS YSDLLSRLIGSEY GG  LSPREVVDECKTFFFGGHETTALAIS
Sbjct: 283 FLSIISNRKMSGSGSGYSDLLSRLIGSEYTGGEALSPREVVDECKTFFFGGHETTALAIS 342

Query: 243 WTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 302
           WTLLLLATSP+WQ ILR EIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ
Sbjct: 343 WTLLLLATSPDWQRILREEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNIQRQ 402

Query: 303 AKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKMGYL 362
           AKGDIDLGD+KIPKGTNIWID+VAMHHDPTLWGDDVN+FNP RFQHDTIHGGC HKMGYL
Sbjct: 403 AKGDIDLGDIKIPKGTNIWIDVVAMHHDPTLWGDDVNDFNPSRFQHDTIHGGCYHKMGYL 462

Query: 363 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 422
           PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP
Sbjct: 463 PFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLILTP 522

Query: 423 LHP 425
           L P
Sbjct: 523 LQP 525

[Close]

BLAST of Chy6G114790 vs. TAIR 10
Match: AT5G52400.1 (cytochrome P450, family 715, subfamily A, polypeptide 1 )

HSP 1 Score: 529.3 bits (1362), Expect = 3.0e-150
Identity = 256/424 (60.38%), Postives = 318/424 (75.00%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGDNW 62
           GK FVYWLG EPF+Y+ADP+ +  +S+ VLGKSWGKP VF+ DREPMFG GLVM EGD+W
Sbjct: 102 GKVFVYWLGIEPFVYVADPEFLSVMSKGVLGKSWGKPNVFKKDREPMFGTGLVMVEGDDW 161

Query: 63  IRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGEIIA 122
            RHRHI+TPAF P NLK MT  MVES + ML+ W   + SG  E ++E EI  TAGEIIA
Sbjct: 162 TRHRHIITPAFAPLNLKVMTNMMVESVSNMLDRWGIQINSGNPEFDMESEIIGTAGEIIA 221

Query: 123 KASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQIDRL 182
           K SFG++    G + L NLRA+Q  LF ++ YVGVPFS+++ Y  +T+ AK LG +ID L
Sbjct: 222 KTSFGVTG-ENGTQVLKNLRAVQFALFNSNRYVGVPFSNILSY-KQTVKAKGLGHEIDGL 281

Query: 183 FFSIISDRKIS---GSGSAYSDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHETTAL 242
             S I+ RKIS   G    + DLL  L+ ++ KG    + +E+VDECKTFFF GHETTAL
Sbjct: 282 LLSFINKRKISLAEGDDQGH-DLLGMLLKADQKG--NFTAKELVDECKTFFFAGHETTAL 341

Query: 243 AISWTLLLLATSPEWQTILRNEIKEVVGDKDIDFSMLSSLKKMGWVWNEVLRLYPSAPNI 302
           A++WT +LLA  PEWQ  +R EI+EV+GD  I+++ L+ LKKM WV NEVLRLYP APN 
Sbjct: 342 ALTWTFMLLAIHPEWQDTIREEIREVIGDSKIEYNKLAGLKKMSWVMNEVLRLYPPAPNA 401

Query: 303 QRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGGCNHKM 362
           QRQA+ DI++    IP GTNIWID+VAMHHD  LWGDDVNEF P+RF    +HGGC +KM
Sbjct: 402 QRQARNDIEVNGRVIPNGTNIWIDVVAMHHDVELWGDDVNEFKPERFD-GNLHGGCKNKM 461

Query: 363 GYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAHGIPLI 422
           GY+PFGFGGRMCIGRNL++MEYKIVL+L+LS F +S+SP Y HSP  +LSLRP +G+PLI
Sbjct: 462 GYMPFGFGGRMCIGRNLTTMEYKIVLSLVLSRFEISVSPGYRHSPTYMLSLRPGYGLPLI 519

Query: 423 LTPL 424
           + PL
Sbjct: 522 IRPL 519

[Close]

BLAST of Chy6G114790 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 288.1 bits (736), Expect = 1.1e-77
Identity = 166/429 (38.69%), Postives = 241/429 (56.18%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKI---SEAVLGKSWGKPAVFRNDREPMFGDGLVMTEG 62
           GK+F+ W GTEP L + + +++K++      V GKSW    + +   +   G GL+M  G
Sbjct: 95  GKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSW----LQQQGTKGFIGRGLLMANG 154

Query: 63  DNWIRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGE 122
           + W   RH+  PAF    LK   K MVE T  M E  R   K    E+E+  E+     +
Sbjct: 155 EAWHHQRHMAAPAFTRDRLKGYAKHMVECTKMMAERLR---KEVGEEVEIGEEMRRLTAD 214

Query: 123 IIAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQI 182
           II++  FG S C  G E  + L  LQ    + + ++  P  S  L        K L  ++
Sbjct: 215 IISRTEFG-SSCDKGKELFSLLTVLQRLCAQATRHLCFP-GSRFLPSKYNREIKSLKTEV 274

Query: 183 DRLFFSIISDRKIS---GSGSAY-SDLLSRLIGSEYKGGAGLSPREVVDECKTFFFGGHE 242
           +RL   II  RK S   G  S+Y  DLL  L+         L+ + ++DECKTFFF GHE
Sbjct: 275 ERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLLNQMDSNKNNLNVQMIMDECKTFFFTGHE 334

Query: 243 TTALAISWTLLLLATSPEWQTILRNEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVLRLYP 302
           TT+L ++WTL+LLA +P WQ  +R+E+++V G   +     LSSL  +  V NE LRLYP
Sbjct: 335 TTSLLLTWTLMLLAHNPTWQDNVRDEVRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYP 394

Query: 303 SAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTIHGG 362
            A  + R A  DI LGD+ IPKG +IWI ++A+HH   LWG+D NEFNP+RF   +    
Sbjct: 395 PATLLPRMAFEDIKLGDLIIPKGLSIWIPVLAIHHSNELWGEDANEFNPERFTTRSF-AS 454

Query: 363 CNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLSLRPAH 422
             H   ++PF  G R CIG+  + ME KI+L +++S FS ++S +Y H+P  +L+++P +
Sbjct: 455 SRH---FMPFAAGPRNCIGQTFAMMEAKIILAMLVSKFSFAISENYRHAPIVVLTIKPKY 510

Query: 423 GIPLILTPL 424
           G+ L+L PL
Sbjct: 515 GVQLVLKPL 510

[Close]

BLAST of Chy6G114790 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 278.9 bits (712), Expect = 6.9e-75
Identity = 160/435 (36.78%), Postives = 240/435 (55.17%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKI---SEAVLGKSWGKPAVFRNDREPMFGDGLVMTEG 62
           GK+F+ W GT+P L + + +L+K++      V G+SW +    +N      G GL+M  G
Sbjct: 94  GKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRSWLQQQGTKN----FIGRGLLMANG 153

Query: 63  DNWIRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSEIEVEREITATAGE 122
            +W   RH+  PAF    LK   + MVE T+K++E  R  V  G +E+E+  E+     +
Sbjct: 154 QDWHHQRHLAAPAFTGERLKGYARHMVECTSKLVERLRKEVGEGANEVEIGEEMHKLTAD 213

Query: 123 IIAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKRLGAQI 182
           II++  FG S    G E  N+L  LQ    + + ++  P  S  L        K L  ++
Sbjct: 214 IISRTKFG-SSFEKGKELFNHLTVLQRRCAQATRHLCFP-GSRFLPSKYNREIKSLKKEV 273

Query: 183 DRLFFSIISDRK---ISGSGSAYSD-----LLSRLIGSEYKGGAGLSPREVVDECKTFFF 242
           +RL   II  R+     G  S + D     LL+ +   +       + + ++DECKTFFF
Sbjct: 274 ERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLLNEMDIDKNNNNNNNNLQLIMDECKTFFF 333

Query: 243 GGHETTALAISWTLLLLATSPEWQTILRNEIKEVVGDKDI-DFSMLSSLKKMGWVWNEVL 302
            GHETTAL ++WT +LLA +P WQ  +R E++EV G   +     LS L  +  V NE L
Sbjct: 334 AGHETTALLLTWTTMLLADNPTWQEKVREEVREVFGRNGLPSVDQLSKLTSLSKVINESL 393

Query: 303 RLYPSAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDT 362
           RLYP A  + R A  D+ LGD+ IPKG +IWI ++A+HH   LWG D N+FNP+RF    
Sbjct: 394 RLYPPATLLPRMAFEDLKLGDLTIPKGLSIWIPVLAIHHSEELWGKDANQFNPERF---- 453

Query: 363 IHGGCNHKMG--YLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLL 422
             GG     G  ++PF  G R CIG+  + ME KI+L  ++S F+ ++S +Y H+P  +L
Sbjct: 454 --GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIILATLISKFNFTISKNYRHAPIVVL 513

Query: 423 SLRPAHGIPLILTPL 424
           +++P +G+ +IL PL
Sbjct: 514 TIKPKYGVQVILKPL 516

[Close]

BLAST of Chy6G114790 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 259.2 bits (661), Expect = 5.7e-69
Identity = 166/433 (38.34%), Postives = 234/433 (54.04%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKISEAVLG---KSWGKPAVFRNDREPMFGDGLVMTEG 62
           G+ F+YW GTEP + I+DP+L K +    LG   KS  +P   +     +   GLV  EG
Sbjct: 93  GETFLYWNGTEPRICISDPELAKTMLSNKLGFFVKSKARPEAVK----LVGSKGLVFIEG 152

Query: 63  DNWIRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWRSLVKSGQSE-----IEVEREIT 122
            +W+RHR IL PAF    LK MT  MV+ T KMLEEWR      ++E      E+  E  
Sbjct: 153 ADWVRHRRILNPAFSIDRLKIMTTVMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQ 212

Query: 123 ATAGEIIAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPARTLAAKR 182
               +IIA ++FG S    G E   +   L+     + + V +P +  +  P+  +   +
Sbjct: 213 RLTADIIATSAFG-SSYVEGIEVFRSQMELKRCYTTSLNQVSIPGTQYLPTPS-NIRVWK 272

Query: 183 LGAQIDRLFFSIISDRKISGSGSAYSDLLSRLIGSEYKGG--AGLSPREVVDECKTFFFG 242
           L  ++D     IIS R  S S     DLL  L+ +    G    +S  E++ EC+TFFFG
Sbjct: 273 LERKMDNSIKRIISSRLQSKSDYG-DDLLGILLKAYNTEGKERKMSIEEIIHECRTFFFG 332

Query: 243 GHETTALAISWTLLLLATSPEWQTILRNEI-KEVVGDKDIDFSMLSSLKKMGWVWNEVLR 302
           GHETT+  ++WT +LL+   +WQ  LR EI KE   +K  D    S LK M  V  E LR
Sbjct: 333 GHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMESLR 392

Query: 303 LYPSAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHDTI 362
           LY     + R+A  +I LGD++IPKGT + I ++ MH D TLWG D ++FNP RF +  +
Sbjct: 393 LYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANG-V 452

Query: 363 HGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFS-LSLSPSYSHSPATLLSL 422
               NH    L F  G R CIG+N   +E K VLT+IL  F  +SL   Y H+P   +++
Sbjct: 453 SRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTI 512

Query: 423 RPAHGIPLILTPL 424
           +P +G+P++L PL
Sbjct: 513 QPQYGLPVMLQPL 517

[Close]

BLAST of Chy6G114790 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 258.8 bits (660), Expect = 7.4e-69
Identity = 148/429 (34.50%), Postives = 238/429 (55.48%), Query Frame = 0


Query: 3   GKKFVYWLGTEPFLYIADPQLVKKI--SEAVLGKSWGKPAVFRNDREPMFGDGLVMTEGD 62
           G  F+ W G    L +ADP L+++I        K+   P V     + + GDGL+  +G+
Sbjct: 96  GATFLVWFGPTFRLTVADPDLIREIFSKSEFYEKNEAHPLV-----KQLEGDGLLSLKGE 155

Query: 63  NWIRHRHILTPAFIPANLKAMTKFMVESTTKMLEEWR-SLVKSGQSEIEVEREITATAGE 122
            W  HR I++P F   NLK +   +++S T M+++W   L ++G+ E++V         +
Sbjct: 156 KWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSENGEVEVDVYEWFQILTED 215

Query: 123 IIAKASFGISKCGGGGEALNNLRALQLTLFKNSSYVGVPFSSLVLYPAR-TLAAKRLGAQ 182
           +I++ +FG S     G A+  L+A Q+ L    ++  V       +P R  L + +L  +
Sbjct: 216 VISRTAFGSSY--EDGRAVFRLQAQQMLLCA-EAFQKVFIPGYRFFPTRGNLKSWKLDKE 275

Query: 183 IDRLFFSIISDRK---ISGSGS-----AYSDLLSRLIGSEYKGGAGLSPREVVDECKTFF 242
           I +    +I  R+   I G G      A  DLL  +I ++      ++ +++V+ECK+FF
Sbjct: 276 IRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMIQAK-----NVTVQDIVEECKSFF 335

Query: 243 FGGHETTALAISWTLLLLATSPEWQTILRNEIKEVVGDKDIDF-SMLSSLKKMGWVWNEV 302
           F G +TT+  ++WT +LL+  PEWQ   R+E+  V G +D+     +  LK +  + NE 
Sbjct: 336 FAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVPTKDHVVKLKTLSMILNES 395

Query: 303 LRLYPSAPNIQRQAKGDIDLGDVKIPKGTNIWIDIVAMHHDPTLWGDDVNEFNPKRFQHD 362
           LRLYP      R+AK D+ LG  KIP GT + I I+A+HHD  +WG+DVNEFNP RF  D
Sbjct: 396 LRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQAIWGNDVNEFNPARFA-D 455

Query: 363 TIHGGCNHKMGYLPFGFGGRMCIGRNLSSMEYKIVLTLILSTFSLSLSPSYSHSPATLLS 419
            +     H +G++PFG G R CIG+NL+ ++ K+ L +++  F+  L+P+Y H+P  L+ 
Sbjct: 456 GVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRFTFHLAPTYQHAPTVLML 510

The following BLAST results are available for this feature:

BLAST of Chy6G114790 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZW95	1.6e-76	38.69	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1	[more]
Q9FF18	9.8e-74	36.78	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1	[more]
Q2QYH7	2.2e-73	36.62	Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 ...	[more]
B6SSW8	3.6e-68	36.15	Cytochrome P450 714B3 OS=Zea mays OX=4577 GN=CYP714B3 PE=2 SV=1	[more]
Q9ASR3	8.0e-68	38.34	Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1	[more]

BLAST of Chy6G114790 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DI76	1.8e-235	95.27	Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold38...	[more]
A0A5A7SWW2	1.5e-234	95.04	Cytokinin hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold23...	[more]
A0A1S3AXX2	1.5e-234	95.04	cytokinin hydroxylase OS=Cucumis melo OX=3656 GN=LOC103484084 PE=3 SV=1	[more]
A0A6J1CIQ8	3.4e-194	79.25	cytokinin hydroxylase OS=Momordica charantia OX=3673 GN=LOC111011538 PE=3 SV=1	[more]
A0A6J1HYD5	1.7e-190	78.69	cytochrome P450 714C2-like OS=Cucurbita maxima OX=3661 GN=LOC111467387 PE=3 SV=1	[more]

BLAST of Chy6G114790 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN57414.2	5.43e-307	98.11	hypothetical protein Csa_010508 [Cucumis sativus]	[more]
XP_004151435.2	1.11e-306	98.11	cytokinin hydroxylase [Cucumis sativus]	[more]
TYK22959.1	3.96e-299	95.27	cytokinin hydroxylase [Cucumis melo var. makuwa]	[more]
KAA0033659.1	6.55e-298	95.04	cytokinin hydroxylase [Cucumis melo var. makuwa]	[more]
XP_008439236.1	6.20e-297	95.04	PREDICTED: cytokinin hydroxylase [Cucumis melo]	[more]

BLAST of Chy6G114790 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52400.1	3.0e-150	60.38	cytochrome P450, family 715, subfamily A, polypeptide 1	[more]
AT1G67110.1	1.1e-77	38.69	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT5G38450.1	6.9e-75	36.78	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]
AT2G46960.2	5.7e-69	38.34	cytochrome P450, family 709, subfamily B, polypeptide 1	[more]
AT2G26710.1	7.4e-69	34.50	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 221..238 score: 26.03 coord: 241..267 score: 33.95 coord: 371..394 score: 32.32 coord: 361..371 score: 51.95
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 362..371 score: 57.77 coord: 284..295 score: 40.99 coord: 371..382 score: 36.49 coord: 232..249 score: 39.24
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 3..399 e-value: 1.9E-78 score: 264.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 2..421 e-value: 2.7E-101 score: 341.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 3..422
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 3..419
None	No IPR available	PANTHER	PTHR24282:SF149	CYTOCHROME P450 FAMILY 72 PROTEIN	coord: 3..419
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 364..373

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy6G114790.1	Chy6G114790.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen