Chy6G108720 (gene) Cucumber (hystrix) v1

Overview


Name	Chy6G108720
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Werner Syndrome-like exonuclease
Location	chrH06: 2284502 .. 2287351 (+)
RNA-Seq Expression	Chy6G108720
Synteny	Chy6G108720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTTCGGGGGAGTAGCAACAGTGGCCATCCTTCGAAGGAGCTCTCTGCATGCTCCAAATCTTTTCCGTCGTGGTCGTTCTCCGCATCGGAGTTCGATCAGCCGTTTACAGAGCAACAACTCCAAGCAATCGAGGCCATTGAAGCTGCTTATCAATCCACCAGCGCCAAAAAACGACGACCAAATTCCACACCCGACGGAAACGAGGTCTCATGCGTTTCTCCCGACACCGGCCGTCGACTGCCTCGTTCCATTTTTTCTCAGCAGTCTCCACATTTTTCTCCCCTATCGCCCTGTCGAGGTATTTTGTTATTTCTACTGTAAAATGTATTGTAGCAGAGGCGGGGAATTTGGGGAACCATGGCTTCTGGTTTTTGAACTATCTTTTGGGGATCTAGGGTTTTTACTTTTGTGCTGAAATTGGAACTATTGGTATGTATGTACTGTTTTGGGTTTCAGTAAACTCAAAGATGAGGTTTCCTGCATTGAATTATGGTGGCCGAATTGTTTACAGTAGAACTGTGAGTGAGGTCGATAGAGCGTCTAGGGAGCTAGCGAAGAAAATCAACTCCACGAGGAAAGCAATGGATCAAATTACTATTGGGTTCGACATCGAGTGGAGACCCTCATTTAAAAGAGGTCAGCAGTTGATTCGATTGAACAAACTTGCACGTGAAATTTGAAGTTTCATACACTACTAGAAAATGCAAATGCTTCTTTCGGTTTCTTGTTACACATTTCTCCTCTCATCTGTCGCTATTTCGCCAAACAATTGATTCGATTACGGTTTTCTTTATTCAAAAGTATGTTAGTTGTGTTTATCGTAGGAACCAAATCTGTAGTGTCTTCAAGAACCAGAAATATTATTTGGAGCAATGTGATGTTCCTTCGTTCATTGAATTTTCTTTTTGTTAAAAGTTTCCCCTCAAGAAATTTATGCTTGACAACAGAACAAATGCATGAAAATATTCATTTCGTCATCAATTAACTTCAATCATGCACAACCTTATTTGATAAGATTGAAATTGTTGCTGGTTCTTATATCTGCGAGAAGTTATTCTTGGAGAGAGCTATGAGGATAGCTCAAGTGGTCAAAACATATATATTTAACCAAAAAGTTAAAGGTTTGAATCTCCTAGTTCCCACATGTTGCTGATCTAAAAGCAAAAGAAAAAGAGAAAGAAAGCGATTTATTGTTTCTTTCTTGCGGTATTTGCTGATTGTTTAATGAAATTACGGCTGAGAATTAGACCTGGATTGTCGATTCCCCCAAGTTCTTTGTGACTATAGTTTGTTGCTTTATCTGGTTTTTGTGGTTAAACTCAAATCTTGGTTGGTTGGTGGCTGGTAACTCAAAAGAACTTAAGCTAATTACTCCTATCATTCTTACAACCTGTTTAATTGGTTGTGGCTTCGGCAAAATCTGTTGATCAAAACTAGAATTTCTACTTCTTTGACTCTTCTAAGATGAATATATACGCAGGTGTTCCACCCGGAAAAGCAGCAGTTATGCAACTGTGCCTGGAAAATTCTGAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCAAAGTTTGCAGGTTCTTCTAGAGGATGACACACTCAGCAAGGTTTGAAACATCACTATAGTTGCTTCTATAAGTTTGTTTTCATATATTACTTAGAAGTTTTAAGCCATTTTAATCAACCATGTCGAATAAACTTCAGGCTGGAGTTGGGATTGCCAGTGATGCTTCAAAAGTTTTTAAAGATTATAACGTGTCTGTCAAACCATTGAACGAAATTTCGGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGGGTCTTCGGGCTCTAACTGAGACGCTTATATCAAAAGAGGTGGTCTATTACTACTACTACTTCTGTTTGTATGAGTTTAAGCTGTTTGAATTGAAATATTTCTTATAAATGTAATAAAGTAAAAAGTTTCGGAAATGTTTTGGAATTCTTTTTGAAGTACTTAAAAAAGAGTTTTTAAATTTTGAAAAATGAGTTAAATCTAAGCCCTTGTTTGGTAAATCATTCTCTTTGTTCTTGGTTTTTAAAAACTAACCTTCTAAAACCTACTTTCTCCTCTTAATTATGTTATATTGCAAACTAAAAGGAGCAACTTTTGAAAACAGAGTTTTTTTTTTTTTTTTTTTGGATTTTCAAAAAGAATCCAAATGTTTCTTCAAAGAAAGTGAAACCAGGTAATAAAATTGAGATAAAACAAGTTTAAATTTAAAAAATCAAGTCATGGTTAACAGAGCTGTAAATTTTCTGCTTGAACGATTTTCAAACATTCCAACCCGTTTACTAGTTACTTAGATGCTGAAAGACAATTTTAATTTTTGAAACTTGTGTAATTCTCTCTTCTTCATCCATAACCCCAATACCATACTCTTATATTCATATGAATGAAGTAAATGTTCATAGAGATCCTAAGCTCAGAGATACTTACAATTTGCAAATCAAATTTTCATCTTTACAATTGAACATTGTAGCTTCAAAAGCCCGACAGAATCAGACTTGGGAACTGGGAGGCGGCTGTGTTGTCAAAGGATCAGCTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACACGAGGTATTATCAAATGACCCTTTTTTCTCTTTTTATGGTAATTAATTCAGAATTTCTTCAAATCTTCATAATCAAAAAAATGTTTGAAGTAACCTTTTCAATTACCTAAAAACTATTGTTCACTCCCAGAGTTCTTTATCATTGTAACAATCTTAACCTCAGATCTTAAAAGGATTTCCACATGTTGAAAAAGTAGCAGATTCAGAGCCAATAGCGTGA

mRNA sequence

ATGGAATTTCGGGGGAGTAGCAACAGTGGCCATCCTTCGAAGGAGCTCTCTGCATGCTCCAAATCTTTTCCGTCGTGGTCGTTCTCCGCATCGGAGTTCGATCAGCCGTTTACAGAGCAACAACTCCAAGCAATCGAGGCCATTGAAGCTGCTTATCAATCCACCAGCGCCAAAAAACGACGACCAAATTCCACACCCGACGGAAACGAGGTCTCATGCGTTTCTCCCGACACCGGCCGTCGACTGCCTCGTTCCATTTTTTCTCAGCAGTCTCCACATTTTTCTCCCCTATCGCCCTGTCGAGTAAACTCAAAGATGAGGTTTCCTGCATTGAATTATGGTGGCCGAATTGTTTACAGTAGAACTGTGAGTGAGGTCGATAGAGCGTCTAGGGAGCTAGCGAAGAAAATCAACTCCACGAGGAAAGCAATGGATCAAATTACTATTGGGTTCGACATCGAGTGGAGACCCTCATTTAAAAGAGGTGTTCCACCCGGAAAAGCAGCAGTTATGCAACTGTGCCTGGAAAATTCTGAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCAAAGTTTGCAGGTTCTTCTAGAGGATGACACACTCAGCAAGGCTGGAGTTGGGATTGCCAGTGATGCTTCAAAAGTTTTTAAAGATTATAACGTGTCTGTCAAACCATTGAACGAAATTTCGGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGGGTCTTCGGGCTCTAACTGAGACGCTTATATCAAAAGAGCTTCAAAAGCCCGACAGAATCAGACTTGGGAACTGGGAGGCGGCTGTGTTGTCAAAGGATCAGCTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACACGAGATCTTAAAAGGATTTCCACATGTTGAAAAAGTAGCAGATTCAGAGCCAATAGCGTGA

Coding sequence (CDS)

ATGGAATTTCGGGGGAGTAGCAACAGTGGCCATCCTTCGAAGGAGCTCTCTGCATGCTCCAAATCTTTTCCGTCGTGGTCGTTCTCCGCATCGGAGTTCGATCAGCCGTTTACAGAGCAACAACTCCAAGCAATCGAGGCCATTGAAGCTGCTTATCAATCCACCAGCGCCAAAAAACGACGACCAAATTCCACACCCGACGGAAACGAGGTCTCATGCGTTTCTCCCGACACCGGCCGTCGACTGCCTCGTTCCATTTTTTCTCAGCAGTCTCCACATTTTTCTCCCCTATCGCCCTGTCGAGTAAACTCAAAGATGAGGTTTCCTGCATTGAATTATGGTGGCCGAATTGTTTACAGTAGAACTGTGAGTGAGGTCGATAGAGCGTCTAGGGAGCTAGCGAAGAAAATCAACTCCACGAGGAAAGCAATGGATCAAATTACTATTGGGTTCGACATCGAGTGGAGACCCTCATTTAAAAGAGGTGTTCCACCCGGAAAAGCAGCAGTTATGCAACTGTGCCTGGAAAATTCTGAATGCCATGTGATGCATATCATTCATTCGGGAATACCTCAAAGTTTGCAGGTTCTTCTAGAGGATGACACACTCAGCAAGGCTGGAGTTGGGATTGCCAGTGATGCTTCAAAAGTTTTTAAAGATTATAACGTGTCTGTCAAACCATTGAACGAAATTTCGGATCTAGCTAATCAAAAGCTTGCTGGAGTTCCCAAAAAATGGGGTCTTCGGGCTCTAACTGAGACGCTTATATCAAAAGAGCTTCAAAAGCCCGACAGAATCAGACTTGGGAACTGGGAGGCGGCTGTGTTGTCAAAGGATCAGCTGCAGTATGCTGCCACAGACGCATTTGCTTCTTGGTATTTACACGAGATCTTAAAAGGATTTCCACATGTTGAAAAAGTAGCAGATTCAGAGCCAATAGCGTGA

Protein sequence

MEFRGSSNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTPDGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYSRTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKGFPHVEKVADSEPIA*

Homology

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BLAST of Chy6G108720 vs. ExPASy Swiss-Prot
Match: Q84LH3 (Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 6.1e-73
Identity = 144/284 (50.70%), Postives = 194/284 (68.31%), Query Frame = 0


Query: 29  SASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTPDGNEVSCVS---------PDTG 88
           S++  D  FTE++L AI+AIEA+Y  + +     ++ P     + V          P+  
Sbjct: 3   SSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNI 62

Query: 89  RR-LPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYSRTVSEVDRASRELAKKIN 148
           RR LPRSI S  S    PLS CR  +   FPA+ +GGRI+YS+T +EVD+ + +L K ++
Sbjct: 63  RRQLPRSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLD 122

Query: 149 STRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQVLL 208
           + R       +G DIEWRPSF++GV PGK A +Q+C++++ C VMHI HSGIPQSLQ L+
Sbjct: 123 TKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLI 182

Query: 209 EDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISK 268
           ED TL K G+GI  D+ K+F DY VS+K + ++SDLANQK+ G  KKWGL +LTETL+ K
Sbjct: 183 EDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCK 242

Query: 269 ELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKGFP 303
           EL KP+RIRLGNWE   LSK QLQYAATDA+ASW+L+++LK  P
Sbjct: 243 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLP 282

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BLAST of Chy6G108720 vs. ExPASy Swiss-Prot
Match: Q14191 (Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2)

HSP 1 Score: 136.7 bits (343), Expect = 4.4e-31
Identity = 71/192 (36.98%), Postives = 115/192 (59.90%), Query Frame = 0


Query: 109 PALNYGGRIVYSRTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKA 168
           P L + G IVYS   S+    S +++  ++      D   +GFD+EW P + RG   GK 
Sbjct: 45  PFLEFTGSIVYSYDASDCSFLSEDISMSLS------DGDVVGFDMEWPPLYNRG-KLGKV 104

Query: 169 AVMQLCLENSECHVMHIIHSGI-PQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKP 228
           A++QLC+  S+C++ H+    + PQ L++LLE+  + KAGVGI  D  K+ +D+++ +K 
Sbjct: 105 ALIQLCVSESKCYLFHVSSMSVFPQGLKMLLENKAVKKAGVGIEGDQWKLLRDFDIKLKN 164

Query: 229 LNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATD 288
             E++D+AN+KL    + W L +L + L+ K+L K   IR  NW    L++DQ  YAATD
Sbjct: 165 FVELTDVANKKLK-CTETWSLNSLVKHLLGKQLLKDKSIRCSNWSKFPLTEDQKLYAATD 224

Query: 289 AFASWYLHEILK 300
           A+A + ++  L+
Sbjct: 225 AYAGFIIYRNLE 228

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BLAST of Chy6G108720 vs. ExPASy Swiss-Prot
Match: O09053 (Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn PE=1 SV=3)

HSP 1 Score: 132.9 bits (333), Expect = 6.4e-30
Identity = 70/191 (36.65%), Postives = 114/191 (59.69%), Query Frame = 0


Query: 109 PALNYGGRIVYSRTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKA 168
           P L + G IVYS   S+    S +++ +++      D   +GFD+EW P +K G    + 
Sbjct: 39  PFLEFPGSIVYSYEASDCSFLSEDISMRLS------DGDVVGFDMEWPPIYKPG-KRSRV 98

Query: 169 AVMQLCLENSECHVMHIIHSGI-PQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKP 228
           AV+QLC+  S+C++ HI    + PQ L++LLE+ ++ KAGVGI  D  K+ +D++V ++ 
Sbjct: 99  AVIQLCVSESKCYLFHISSMSVFPQGLKMLLENKSIKKAGVGIEGDQWKLLRDFDVKLES 158

Query: 229 LNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATD 288
             E++D+AN+KL    + W L  L + ++ K+L K   IR  NW    L++DQ  YAATD
Sbjct: 159 FVELTDVANEKLK-CAETWSLNGLVKHVLGKQLLKDKSIRCSNWSNFPLTEDQKLYAATD 218

Query: 289 AFASWYLHEIL 299
           A+A   +++ L
Sbjct: 219 AYAGLIIYQKL 221

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BLAST of Chy6G108720 vs. ExPASy Swiss-Prot
Match: O93530 (Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 6.4e-30
Identity = 68/193 (35.23%), Postives = 113/193 (58.55%), Query Frame = 0


Query: 109 PALNYGGRIVYSRTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKA 168
           P + + G IVYS   ++    S ++   +      +++  +GFDIEW P + +G   GK 
Sbjct: 39  PFMKFNGSIVYSYESNDCSLLSEDIRSSL------LEEDVLGFDIEWPPVYTKG-KTGKV 98

Query: 169 AVMQLCLENSECHVMHII-HSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKP 228
           A++Q+C+   +C++ HI   +G P+ L+ LLED+++ K GVGI  D  K+  DY + +K 
Sbjct: 99  ALIQVCVSEKKCYLFHISPMAGFPKGLKRLLEDESVRKVGVGIEGDQWKLMSDYELKLKG 158

Query: 229 LNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATD 288
             E+S++ANQKL    +KW    L + L  ++L K    R  NW+  +L++DQ  YAATD
Sbjct: 159 FIELSEMANQKLR-CKEKWTFNGLIKHLFKEQLYKRKSYRCSNWDIFLLTEDQKLYAATD 218

Query: 289 AFASWYLHEILKG 301
           A+A   +++ L+G
Sbjct: 219 AYAGLLIYKKLEG 223

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BLAST of Chy6G108720 vs. ExPASy Swiss-Prot
Match: Q9VGN7 (Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227 GN=Exd2 PE=2 SV=2)

HSP 1 Score: 78.6 bits (192), Expect = 1.4e-13
Identity = 54/151 (35.76%), Postives = 75/151 (49.67%), Query Frame = 0


Query: 149 IGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIH-SGIPQSLQVLLEDDTLSKAG 208
           +GFD EW      G      A++QL      C +  + H   IPQ L+ LLEDD++ K G
Sbjct: 80  LGFDCEW---ITVGGSRRPVALLQLSSHRGLCALFRLCHMKQIPQDLRELLEDDSVIKVG 139

Query: 209 VGIASDASKVFKDYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISKELQKPDRIR 268
           V    DA K+  DY V V    ++  L    +AG  K  GL  L++T ++  L K  R+ 
Sbjct: 140 VAPQEDAMKLSHDYGVGVASTLDLRFLC--VMAG-HKPEGLGKLSKTHLNYTLDKHWRLA 199

Query: 269 LGNWEAAVLSKDQLQYAATDAFASWYLHEIL 299
             NWEA  L   QL YAA DA  +  +++ L
Sbjct: 200 CSNWEAKTLEPKQLDYAANDALMAVAIYQKL 224

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BLAST of Chy6G108720 vs. ExPASy TrEMBL
Match: A0A0A0L9B9 (3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G129680 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 3.3e-170
Identity = 303/314 (96.50%), Postives = 305/314 (97.13%), Query Frame = 0


Query: 1   MEFRGSSNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFRGSSNS H S ELSACS SFP WSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYS 120
           RPNSTPDGNEVSCVSPDTGRRLPRSIFS QSP FSPLSPCRVNSKMRFPALNYGGRI+YS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIPQSLQ LLEDDTLSKAGVGIASDASKVFK+YNVSVKPLNEISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKELQKPDRIRLGNWE AVLSKDQLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 315
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

[Close]

BLAST of Chy6G108720 vs. ExPASy TrEMBL
Match: A0A1S3AWQ6 (Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103483463 PE=4 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 291/314 (92.68%), Postives = 301/314 (95.86%), Query Frame = 0


Query: 1   MEFRGSSNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF GSSNSGH S ELSACS SFPSWSFS+SEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYS 120
           RPNST D N VSCVSP+TGRRLPRSIFSQQSPHFSPLS CRVNSKMRFPAL+YGGRI+YS
Sbjct: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRK M+QITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIPQSLQ LLEDDTL+KAGVGIASDASKVFKDYNVS+K L+EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKEL+KPDRIRLGNWE AVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 315
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

[Close]

BLAST of Chy6G108720 vs. ExPASy TrEMBL
Match: A0A5A7TZU7 (Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002920 PE=4 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.9e-160
Identity = 286/308 (92.86%), Postives = 296/308 (96.10%), Query Frame = 0


Query: 7   SNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTP 66
           SNSGH S ELSACS SFPSWSFS+SEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNST 
Sbjct: 32  SNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTL 91

Query: 67  DGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYSRTVSEV 126
           D N VSCVSP+TGRRLPRSIFSQQSPHFSPLS CRVNSKMRFPAL+YGGRI+YSRTVSEV
Sbjct: 92  DENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEV 151

Query: 127 DRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII 186
           DRASRELAKKINSTRK M+QITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII
Sbjct: 152 DRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII 211

Query: 187 HSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLAGVPKKW 246
           HSGIPQSLQ LLEDDTL+KAGVGIASDASKVFKDYNVS+K L+EISDLANQKLAGVPKKW
Sbjct: 212 HSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLAGVPKKW 271

Query: 247 GLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKGFPHVEK 306
           GLRALTETLISKEL+KPDRIRLGNWE AVLSK+QL YAATDAFASWYLHEILKGFPHVEK
Sbjct: 272 GLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVEK 331

Query: 307 VADSEPIA 315
           VADSEPIA
Sbjct: 332 VADSEPIA 339

[Close]

BLAST of Chy6G108720 vs. ExPASy TrEMBL
Match: A0A6J1G5L3 (Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451092 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 1.0e-131
Identity = 243/311 (78.14%), Postives = 267/311 (85.85%), Query Frame = 0


Query: 1   MEFRGSSNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF  SSN    S ELS CS    S+SFS SEFDQPFTEQQLQAIEAIEAAYQ+T+AK+R
Sbjct: 1   MEFPCSSNRAGASTELSVCSSPSSSYSFSLSEFDQPFTEQQLQAIEAIEAAYQATTAKRR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYS 120
           R       NEV CVSP++GRRLPRSI S QSPH SPLSPCRV SKMRFPAL +GGRIVYS
Sbjct: 61  RE------NEVLCVSPNSGRRLPRSISSLQSPHSSPLSPCRVTSKMRFPALKFGGRIVYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RT SEVDRASRELAKKINS RK MDQITIGFDIEW+P+F+RGVPPGKAAVMQLCL++S+C
Sbjct: 121 RTASEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDHSQC 180

Query: 181 HVMHIIHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIP+SLQ LLEDDTL+KAGVGI +DA KVFKDYNVS+K ++EIS+LANQKLA
Sbjct: 181 HVMHIIHSGIPRSLQALLEDDTLNKAGVGIVNDAMKVFKDYNVSIKAVDEISNLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKG 300
           GVPKKW L +LTE LISKEL KP +IRLGNWE AVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWSLGSLTEALISKELPKPSKIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSE 312
           FP  +  ADSE
Sbjct: 301 FPDPQAAADSE 305

[Close]

BLAST of Chy6G108720 vs. ExPASy TrEMBL
Match: A0A6J1D021 (Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016124 PE=4 SV=1)

HSP 1 Score: 478.8 bits (1231), Expect = 1.8e-131
Identity = 240/311 (77.17%), Postives = 266/311 (85.53%), Query Frame = 0


Query: 1   MEFRGSSNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF G S +   S  LS  S SF S SFS SEFDQPFTEQQLQ I+AIEA YQSTS K+R
Sbjct: 1   MEFPGCSRNAGASPPLSVSSSSFSSRSFSVSEFDQPFTEQQLQEIDAIEAMYQSTSGKRR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYS 120
           R +ST D NE SCVSP+ GRRLP SI S Q P+ SPLSP RVNSKMRFPALN+ GRI+YS
Sbjct: 61  RGSSTADENEASCVSPNAGRRLPHSILSLQLPYSSPLSPRRVNSKMRFPALNFDGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
            T SEVDRASRELAKKI+STRK  DQITIGFDIEW+P+F+RGVPPGK AVMQLCL+NS+C
Sbjct: 121 TTASEVDRASRELAKKISSTRKGTDQITIGFDIEWKPTFRRGVPPGKTAVMQLCLDNSQC 180

Query: 181 HVMHIIHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGI  SL+ LLEDDTLSKAGVGIA+DA KVFKDYNVS+K ++EISDLANQKLA
Sbjct: 181 HVMHIIHSGITPSLKTLLEDDTLSKAGVGIANDAVKVFKDYNVSIKAVSEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKG 300
           GVP+KWGL +LT+ L+SKELQKP++IRLGNWE AVLSK QLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPQKWGLGSLTKALVSKELQKPNKIRLGNWEVAVLSKQQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSE 312
           FPH +KV DSE
Sbjct: 301 FPHAQKVEDSE 311

[Close]

BLAST of Chy6G108720 vs. NCBI nr
Match: XP_004133981.1 (Werner Syndrome-like exonuclease isoform X1 [Cucumis sativus] >KGN56711.1 hypothetical protein Csa_010757 [Cucumis sativus])

HSP 1 Score: 604 bits (1557), Expect = 2.00e-217
Identity = 303/314 (96.50%), Postives = 305/314 (97.13%), Query Frame = 0


Query: 1   MEFRGSSNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFRGSSNS H S ELSACS SFP WSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYS 120
           RPNSTPDGNEVSCVSPDTGRRLPRSIFS QSP FSPLSPCRVNSKMRFPALNYGGRI+YS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIPQSLQ LLEDDTLSKAGVGIASDASKVFK+YNVSVKPLNEISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKELQKPDRIRLGNWE AVLSKDQLQYAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 314
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

[Close]

BLAST of Chy6G108720 vs. NCBI nr
Match: XP_008438323.1 (PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo])

HSP 1 Score: 579 bits (1492), Expect = 1.61e-207
Identity = 291/314 (92.68%), Postives = 301/314 (95.86%), Query Frame = 0


Query: 1   MEFRGSSNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF GSSNSGH S ELSACS SFPSWSFS+SEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFPGSSNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYS 120
           RPNST D N VSCVSP+TGRRLPRSIFSQQSPHFSPLS CRVNSKMRFPAL+YGGRI+YS
Sbjct: 61  RPNSTLDENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRK M+QITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIPQSLQ LLEDDTL+KAGVGIASDASKVFKDYNVS+K L+EISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKG 300
           GVPKKWGLRALTETLISKEL+KPDRIRLGNWE AVLSK+QL YAATDAFASWYLHEILKG
Sbjct: 241 GVPKKWGLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPIA 314
           FPHVEKVADSEPIA
Sbjct: 301 FPHVEKVADSEPIA 314

[Close]

BLAST of Chy6G108720 vs. NCBI nr
Match: XP_031738583.1 (Werner Syndrome-like exonuclease isoform X2 [Cucumis sativus])

HSP 1 Score: 569 bits (1466), Expect = 8.81e-204
Identity = 286/297 (96.30%), Postives = 288/297 (96.97%), Query Frame = 0


Query: 1   MEFRGSSNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEFRGSSNS H S ELSACS SFP WSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR
Sbjct: 1   MEFRGSSNSSHSSTELSACSSSFPPWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYS 120
           RPNSTPDGNEVSCVSPDTGRRLPRSIFS QSP FSPLSPCRVNSKMRFPALNYGGRI+YS
Sbjct: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSHQSPRFSPLSPCRVNSKMRFPALNYGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC
Sbjct: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180

Query: 181 HVMHIIHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIPQSLQ LLEDDTLSKAGVGIASDASKVFK+YNVSVKPLNEISDLANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLSKAGVGIASDASKVFKEYNVSVKPLNEISDLANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEI 297
           GVPKKWGLRALTETLISKELQKPDRIRLGNWE AVLSKDQLQYAATDAFASWYLHEI
Sbjct: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEVAVLSKDQLQYAATDAFASWYLHEI 297

[Close]

BLAST of Chy6G108720 vs. NCBI nr
Match: KAA0049133.1 (Werner Syndrome-like exonuclease [Cucumis melo var. makuwa] >TYK17428.1 Werner Syndrome-like exonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 570 bits (1468), Expect = 1.84e-203
Identity = 286/308 (92.86%), Postives = 296/308 (96.10%), Query Frame = 0


Query: 7   SNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTP 66
           SNSGH S ELSACS SFPSWSFS+SEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNST 
Sbjct: 32  SNSGHSSTELSACSSSFPSWSFSSSEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTL 91

Query: 67  DGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYSRTVSEV 126
           D N VSCVSP+TGRRLPRSIFSQQSPHFSPLS CRVNSKMRFPAL+YGGRI+YSRTVSEV
Sbjct: 92  DENGVSCVSPNTGRRLPRSIFSQQSPHFSPLSSCRVNSKMRFPALSYGGRIIYSRTVSEV 151

Query: 127 DRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII 186
           DRASRELAKKINSTRK M+QITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII
Sbjct: 152 DRASRELAKKINSTRKGMEQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII 211

Query: 187 HSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLAGVPKKW 246
           HSGIPQSLQ LLEDDTL+KAGVGIASDASKVFKDYNVS+K L+EISDLANQKLAGVPKKW
Sbjct: 212 HSGIPQSLQALLEDDTLNKAGVGIASDASKVFKDYNVSMKQLDEISDLANQKLAGVPKKW 271

Query: 247 GLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKGFPHVEK 306
           GLRALTETLISKEL+KPDRIRLGNWE AVLSK+QL YAATDAFASWYLHEILKGFPHVEK
Sbjct: 272 GLRALTETLISKELRKPDRIRLGNWEVAVLSKEQLHYAATDAFASWYLHEILKGFPHVEK 331

Query: 307 VADSEPIA 314
           VADSEPIA
Sbjct: 332 VADSEPIA 339

[Close]

BLAST of Chy6G108720 vs. NCBI nr
Match: XP_038897657.1 (Werner Syndrome-like exonuclease [Benincasa hispida])

HSP 1 Score: 531 bits (1369), Expect = 8.57e-189
Identity = 264/313 (84.35%), Postives = 285/313 (91.05%), Query Frame = 0


Query: 1   MEFRGSSNSGHPSKELSACSKSFPSWSFSASEFDQPFTEQQLQAIEAIEAAYQSTSAKKR 60
           MEF  SSN GH S +LS CS S  SWSFSASEFDQPFTEQQLQ IEAIEAAYQSTSAKKR
Sbjct: 1   MEFHCSSNGGHSSTKLSVCSSS-SSWSFSASEFDQPFTEQQLQEIEAIEAAYQSTSAKKR 60

Query: 61  RPNSTPDGNEVSCVSPDTGRRLPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYS 120
           RPNSTPD NEVSCVSPDTGRRLPRSI S QSPH SPLSPCRVNSKMRFPALN+GGRI+YS
Sbjct: 61  RPNSTPDENEVSCVSPDTGRRLPRSILSHQSPHSSPLSPCRVNSKMRFPALNFGGRIIYS 120

Query: 121 RTVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSEC 180
           RT SEVDRASRELAKKINS RK MDQITIGFDIEW+P+F+RGVPPGKAAVMQLCL+NS+C
Sbjct: 121 RTWSEVDRASRELAKKINSMRKGMDQITIGFDIEWKPTFRRGVPPGKAAVMQLCLDNSQC 180

Query: 181 HVMHIIHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLA 240
           HVMHIIHSGIPQSLQ LLEDDTL+KAGVGI +DA+KV KDYNVS+K ++EISD+ANQKLA
Sbjct: 181 HVMHIIHSGIPQSLQALLEDDTLTKAGVGIVNDATKVLKDYNVSIKAVDEISDIANQKLA 240

Query: 241 GVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKG 300
           G P+KW L +LTETLISKELQKP++IRLGNWE AVLS +QLQYAATDAFASWYLHEILKG
Sbjct: 241 GFPRKWSLGSLTETLISKELQKPNKIRLGNWEVAVLSTEQLQYAATDAFASWYLHEILKG 300

Query: 301 FPHVEKVADSEPI 313
           FPH +KVAD EPI
Sbjct: 301 FPHAQKVADPEPI 312

[Close]

BLAST of Chy6G108720 vs. TAIR 10
Match: AT4G13870.2 (Werner syndrome-like exonuclease )

HSP 1 Score: 275.8 bits (704), Expect = 4.3e-74
Identity = 144/284 (50.70%), Postives = 194/284 (68.31%), Query Frame = 0


Query: 29  SASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTPDGNEVSCVS---------PDTG 88
           S++  D  FTE++L AI+AIEA+Y  + +     ++ P     + V          P+  
Sbjct: 3   SSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNI 62

Query: 89  RR-LPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYSRTVSEVDRASRELAKKIN 148
           RR LPRSI S  S    PLS CR  +   FPA+ +GGRI+YS+T +EVD+ + +L K ++
Sbjct: 63  RRQLPRSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLD 122

Query: 149 STRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQVLL 208
           + R       +G DIEWRPSF++GV PGK A +Q+C++++ C VMHI HSGIPQSLQ L+
Sbjct: 123 TKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLI 182

Query: 209 EDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISK 268
           ED TL K G+GI  D+ K+F DY VS+K + ++SDLANQK+ G  KKWGL +LTETL+ K
Sbjct: 183 EDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCK 242

Query: 269 ELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKGFP 303
           EL KP+RIRLGNWE   LSK QLQYAATDA+ASW+L+++LK  P
Sbjct: 243 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKVLKDLP 282

[Close]

BLAST of Chy6G108720 vs. TAIR 10
Match: AT4G13870.1 (Werner syndrome-like exonuclease )

HSP 1 Score: 268.9 bits (686), Expect = 5.3e-72
Identity = 142/280 (50.71%), Postives = 191/280 (68.21%), Query Frame = 0


Query: 29  SASEFDQPFTEQQLQAIEAIEAAYQSTSAKKRRPNSTPDGNEVSCVS---------PDTG 88
           S++  D  FTE++L AI+AIEA+Y  + +     ++ P     + V          P+  
Sbjct: 3   SSNWIDDAFTEEELLAIDAIEASYNFSRSSSSSSSAAPTVQATTSVHGHEEDPNQIPNNI 62

Query: 89  RR-LPRSIFSQQSPHFSPLSPCRVNSKMRFPALNYGGRIVYSRTVSEVDRASRELAKKIN 148
           RR LPRSI S  S    PLS CR  +   FPA+ +GGRI+YS+T +EVD+ + +L K ++
Sbjct: 63  RRQLPRSITSSTSYKRFPLSRCRARN---FPAMRFGGRILYSKTATEVDKRAMQLIKVLD 122

Query: 149 STRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHIIHSGIPQSLQVLL 208
           + R       +G DIEWRPSF++GV PGK A +Q+C++++ C VMHI HSGIPQSLQ L+
Sbjct: 123 TKRDESGIAFVGLDIEWRPSFRKGVLPGKVATVQICVDSNYCDVMHIFHSGIPQSLQHLI 182

Query: 209 EDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLAGVPKKWGLRALTETLISK 268
           ED TL K G+GI  D+ K+F DY VS+K + ++SDLANQK+ G  KKWGL +LTETL+ K
Sbjct: 183 EDSTLVKVGIGIDGDSVKLFHDYGVSIKDVEDLSDLANQKIGG-DKKWGLASLTETLVCK 242

Query: 269 ELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEIL 299
           EL KP+RIRLGNWE   LSK QLQYAATDA+ASW+L++ L
Sbjct: 243 ELLKPNRIRLGNWEFYPLSKQQLQYAATDAYASWHLYKDL 278

[Close]

BLAST of Chy6G108720 vs. TAIR 10
Match: AT4G13885.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 84.0 bits (206), Expect = 2.4e-16
Identity = 47/109 (43.12%), Postives = 56/109 (51.38%), Query Frame = 0


Query: 194 LQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEISDLANQKLAGVPKKWGLRALTE 253
           LQ L+ED TL K G+GI  D+ K+F DY                                
Sbjct: 186 LQHLIEDSTLVKVGIGIDGDSVKLFHDYG------------------------------- 245

Query: 254 TLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHEILKGFP 303
                 L KP+RIRLGNWE   LSK QLQYAATDA+ASW+LH++LK  P
Sbjct: 246 ------LLKPNRIRLGNWEVHPLSKQQLQYAATDAYASWHLHQVLKDLP 257

[Close]

BLAST of Chy6G108720 vs. TAIR 10
Match: AT1G56310.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.7e-07
Identity = 44/186 (23.66%), Postives = 83/186 (44.62%), Query Frame = 0


Query: 136 KINSTRKAMDQI----TIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECHVMHII----- 195
           ++N  RKA   +     +G D EW+P++ +G    K ++MQ+   +++  ++ +I     
Sbjct: 364 EVNELRKATSFLEGCRVVGIDCEWKPNYIKGSKQNKVSIMQIG-SDTKIFILDLIKLYND 423

Query: 196 -HSGIPQSLQVLLEDDTLSKAGVGIASDAS---------KVFKDYNVSVKPLNEISDLAN 255
               +   L  +L+  +  K G     D           K F+ Y++    L +I ++ N
Sbjct: 424 ASEILDNCLSHILQSKSTLKLGYNFQCDIKQLALSYGDLKCFERYDM----LLDIQNVFN 483

Query: 256 QKLAGVPKKWGLRALTETLISKELQKPDRIRLGNWEAAVLSKDQLQYAATDAFASWYLHE 303
           +         GL  LT+ ++   L K    R  +WE   LS++QL+YAA DA    ++  
Sbjct: 484 EPFG------GLAGLTKKILGVSLNKTR--RNSDWEQRPLSQNQLEYAALDAAVLIHIFR 536

[Close]

BLAST of Chy6G108720 vs. TAIR 10
Match: AT5G48350.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.9e-05
Identity = 46/194 (23.71%), Postives = 83/194 (42.78%), Query Frame = 0


Query: 122 TVSEVDRASRELAKKINSTRKAMDQITIGFDIEWRPSFKRGVPPGKAAVMQLCLENSECH 181
           TV+E +R    L K   S +    +I IG D E     ++G    K  ++QLC +   C 
Sbjct: 16  TVTEKERDINRLVKTFLSNKNNRKKI-IGLDTE---RVQKGRKLNKTVLLQLC-DGDNCL 75

Query: 182 VMHI----------IHSGIPQSLQVLLEDDTLSKAGVGIASDASKVFKDYNVSVKPLNEI 241
           ++ +              +P  L   L     +  G+GI     ++  ++ ++ K + EI
Sbjct: 76  IVQLPDEDEDEGEGEDDNLPLPLFNFLNLPEFTFVGIGINKTMMRLESEFGLTCKNVVEI 135

Query: 242 SDLANQKLAGVPKKWGLRALT-------ETLISKELQKPDRIRLGNWEAAVLSKDQLQYA 299
                      P  W L  +T         ++S E  +P    L +WE  VL+K+Q++ A
Sbjct: 136 G----------PATWNLTNMTTDVKFRISAIVSTE--RPSNAVLEDWEKFVLNKNQIKLA 192

The following BLAST results are available for this feature:

BLAST of Chy6G108720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84LH3	6.1e-73	50.70	Werner Syndrome-like exonuclease OS=Arabidopsis thaliana OX=3702 GN=WEX PE=1 SV=...	[more]
Q14191	4.4e-31	36.98	Werner syndrome ATP-dependent helicase OS=Homo sapiens OX=9606 GN=WRN PE=1 SV=2	[more]
O09053	6.4e-30	36.65	Werner syndrome ATP-dependent helicase homolog OS=Mus musculus OX=10090 GN=Wrn P...	[more]
O93530	6.4e-30	35.23	Werner syndrome ATP-dependent helicase homolog OS=Xenopus laevis OX=8355 GN=wrn ...	[more]
Q9VGN7	1.4e-13	35.76	Exonuclease 3'-5' domain-containing protein 2 OS=Drosophila melanogaster OX=7227...	[more]

BLAST of Chy6G108720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9B9	3.3e-170	96.50	3'-5' exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]
A0A1S3AWQ6	1.1e-162	92.68	Werner Syndrome-like exonuclease OS=Cucumis melo OX=3656 GN=LOC103483463 PE=4 SV...	[more]
A0A5A7TZU7	6.9e-160	92.86	Werner Syndrome-like exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A6J1G5L3	1.0e-131	78.14	Werner Syndrome-like exonuclease OS=Cucurbita moschata OX=3662 GN=LOC111451092 P...	[more]
A0A6J1D021	1.8e-131	77.17	Werner Syndrome-like exonuclease OS=Momordica charantia OX=3673 GN=LOC111016124 ...	[more]

BLAST of Chy6G108720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004133981.1	2.00e-217	96.50	Werner Syndrome-like exonuclease isoform X1 [Cucumis sativus] >KGN56711.1 hypoth...	[more]
XP_008438323.1	1.61e-207	92.68	PREDICTED: Werner Syndrome-like exonuclease [Cucumis melo]	[more]
XP_031738583.1	8.81e-204	96.30	Werner Syndrome-like exonuclease isoform X2 [Cucumis sativus]	[more]
KAA0049133.1	1.84e-203	92.86	Werner Syndrome-like exonuclease [Cucumis melo var. makuwa] >TYK17428.1 Werner S...	[more]
XP_038897657.1	8.57e-189	84.35	Werner Syndrome-like exonuclease [Benincasa hispida]	[more]

BLAST of Chy6G108720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G13870.2	4.3e-74	50.70	Werner syndrome-like exonuclease	[more]
AT4G13870.1	5.3e-72	50.71	Werner syndrome-like exonuclease	[more]
AT4G13885.1	2.4e-16	43.12	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G56310.1	2.7e-07	23.66	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT5G48350.1	1.9e-05	23.71	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002562	3'-5' exonuclease domain	SMART	SM00474	35exoneu6	coord: 117..303 e-value: 1.4E-17 score: 74.4
IPR002562	3'-5' exonuclease domain	PFAM	PF01612	DNA_pol_A_exo1	coord: 135..299 e-value: 3.8E-16 score: 59.3
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 101..301 e-value: 1.2E-51 score: 176.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..91
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..72
None	No IPR available	PANTHER	PTHR13620:SF45	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 46..306
None	No IPR available	PANTHER	PTHR13620	3-5 EXONUCLEASE	coord: 46..306
None	No IPR available	CDD	cd06141	WRN_exo	coord: 121..299 e-value: 1.89714E-56 score: 178.541
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 147..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy6G108720.1	Chy6G108720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0006139	nucleobase-containing compound metabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008408	3'-5' exonuclease activity
molecular_function	GO:0003676	nucleic acid binding