Chy5G092540 (gene) Cucumber (hystrix) v1

Overview
NameChy5G092540
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPoly(A)-specific ribonuclease
LocationchrH05: 1754876 .. 1755673 (-)
RNA-Seq ExpressionChy5G092540
SyntenyChy5G092540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAATCCAAACCCGTCAGGTCTGGGATCTCAATCTCCTTCATGAATTCAATCTCATCACTCAACTCCTCCACCGCTACCCTTTCATCTCCATCGACACTGAATTCCCAGGTGTCCTCATCCGCCCCACCCTCCACCGCCACCCTCTCCGCCCTTCCGATCACTACCTCCTCCTCAAGTCCAACGTCGACGCTCTCAACCTAATCCAACTCGGTCTCACCCTCTCCGACGCCGACGGCAACCTCCCCACACTCGGCACCAAAAACTCCTTCATCTGGGAGTTCAATTTCAGAGATTTCGACGTTGCACGCGACCCCCACGCCCCGGATTCCATCGAGCTCTTAAAACAACAGGGGATTGATTTCCACCGGAACAGAACTCATGGGGTTTGCTCCTCCCGGTTCGCCGACTTGCTGATGTCATCCGGTCTTTTGTGCAATGACTCCCTCACTTGGGTTACCTTTCACAGCGCCTATGACTTTGGTTACTTGGTTAAGATTCTCACCCGGACCAAACTGCCGTCTCGGCTTCAGGATTTCTTGAGCATTCTCAAAGGGTTGTTTGGGAAAAAGGTATATGACGTGAAGCATATGATGCGGTTCTGTGATGGGCTTTATGGGGGTTTGGACCGCCTTGCTAAGACCCTTGATTTGGACCGGGCGGTTGGGAAATCCCATCAGGCCGGTTCTGATTCCCTCCTCACATTGCAAGCTTTTAAGAAGATGAGTGATGTTTACTTCTCAAAGGATGGCCCGGACAAGCACGCCGGCGTCTTGTTTGGTTTAGAATTGTATTGA

mRNA sequence

ATGTTAATCCAAACCCGTCAGGTCTGGGATCTCAATCTCCTTCATGAATTCAATCTCATCACTCAACTCCTCCACCGCTACCCTTTCATCTCCATCGACACTGAATTCCCAGGTGTCCTCATCCGCCCCACCCTCCACCGCCACCCTCTCCGCCCTTCCGATCACTACCTCCTCCTCAAGTCCAACGTCGACGCTCTCAACCTAATCCAACTCGGTCTCACCCTCTCCGACGCCGACGGCAACCTCCCCACACTCGGCACCAAAAACTCCTTCATCTGGGAGTTCAATTTCAGAGATTTCGACGTTGCACGCGACCCCCACGCCCCGGATTCCATCGAGCTCTTAAAACAACAGGGGATTGATTTCCACCGGAACAGAACTCATGGGGTTTGCTCCTCCCGGTTCGCCGACTTGCTGATGTCATCCGGTCTTTTGTGCAATGACTCCCTCACTTGGGTTACCTTTCACAGCGCCTATGACTTTGGTTACTTGGTTAAGATTCTCACCCGGACCAAACTGCCGTCTCGGCTTCAGGATTTCTTGAGCATTCTCAAAGGGTTGTTTGGGAAAAAGGTATATGACGTGAAGCATATGATGCGGTTCTGTGATGGGCTTTATGGGGGTTTGGACCGCCTTGCTAAGACCCTTGATTTGGACCGGGCGGTTGGGAAATCCCATCAGGCCGGTTCTGATTCCCTCCTCACATTGCAAGCTTTTAAGAAGATGAGTGATGTTTACTTCTCAAAGGATGGCCCGGACAAGCACGCCGGCGTCTTGTTTGGTTTAGAATTGTATTGA

Coding sequence (CDS)

ATGTTAATCCAAACCCGTCAGGTCTGGGATCTCAATCTCCTTCATGAATTCAATCTCATCACTCAACTCCTCCACCGCTACCCTTTCATCTCCATCGACACTGAATTCCCAGGTGTCCTCATCCGCCCCACCCTCCACCGCCACCCTCTCCGCCCTTCCGATCACTACCTCCTCCTCAAGTCCAACGTCGACGCTCTCAACCTAATCCAACTCGGTCTCACCCTCTCCGACGCCGACGGCAACCTCCCCACACTCGGCACCAAAAACTCCTTCATCTGGGAGTTCAATTTCAGAGATTTCGACGTTGCACGCGACCCCCACGCCCCGGATTCCATCGAGCTCTTAAAACAACAGGGGATTGATTTCCACCGGAACAGAACTCATGGGGTTTGCTCCTCCCGGTTCGCCGACTTGCTGATGTCATCCGGTCTTTTGTGCAATGACTCCCTCACTTGGGTTACCTTTCACAGCGCCTATGACTTTGGTTACTTGGTTAAGATTCTCACCCGGACCAAACTGCCGTCTCGGCTTCAGGATTTCTTGAGCATTCTCAAAGGGTTGTTTGGGAAAAAGGTATATGACGTGAAGCATATGATGCGGTTCTGTGATGGGCTTTATGGGGGTTTGGACCGCCTTGCTAAGACCCTTGATTTGGACCGGGCGGTTGGGAAATCCCATCAGGCCGGTTCTGATTCCCTCCTCACATTGCAAGCTTTTAAGAAGATGAGTGATGTTTACTTCTCAAAGGATGGCCCGGACAAGCACGCCGGCGTCTTGTTTGGTTTAGAATTGTATTGA

Protein sequence

MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLSILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKMSDVYFSKDGPDKHAGVLFGLELY*
Homology
BLAST of Chy5G092540 vs. ExPASy Swiss-Prot
Match: Q9FMS6 (Probable CCR4-associated factor 1 homolog 11 OS=Arabidopsis thaliana OX=3702 GN=CAF1-11 PE=2 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.3e-97
Identity = 175/268 (65.30%), Postives = 218/268 (81.34%), Query Frame = 0

Query: 2   LIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLH-RHPLRPSDHYLLLK 61
           +I  R VW  NL  EF+LI  ++  YPFIS+DTEFPGV+ +  L       P+  Y LLK
Sbjct: 11  VIVIRDVWAYNLESEFDLIRGIVEDYPFISMDTEFPGVIYKADLDVLRRGNPNYLYNLLK 70

Query: 62  SNVDALNLIQLGLTLSDADGNLPTLGTKNS--FIWEFNFRDFDVARDPHAPDSIELLKQQ 121
           SNVDAL+LIQ+GLTLSDADGNLP LG + +  +IWEFNFRDFDV RDPHAPDSIELL++ 
Sbjct: 71  SNVDALSLIQVGLTLSDADGNLPDLGGQKNRRYIWEFNFRDFDVERDPHAPDSIELLRRH 130

Query: 122 GIDFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQ 181
           GIDF RNR  GV S RFA+L+MSSGL+CN+S++WVTFHSAYDFGYLVKILTR +LP  L+
Sbjct: 131 GIDFERNRREGVESERFAELMMSSGLICNESVSWVTFHSAYDFGYLVKILTRRQLPVALR 190

Query: 182 DFLSILKGLFGKKVYDVKHMMRFCD-GLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQ 241
           +FL +L+  FG +VYDVKH+MRFC+  LYGGLDR+A++L+++RAVGK HQAGSDSLLT Q
Sbjct: 191 EFLGLLRAFFGDRVYDVKHIMRFCEQRLYGGLDRVARSLEVNRAVGKCHQAGSDSLLTWQ 250

Query: 242 AFKKMSDVYFSKDGPDKHAGVLFGLELY 266
           AF++M D+YF +DG +KHAGVL+GLE++
Sbjct: 251 AFQRMRDLYFVEDGAEKHAGVLYGLEVF 278

BLAST of Chy5G092540 vs. ExPASy Swiss-Prot
Match: Q9LXM2 (Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=CAF1-9 PE=2 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 5.1e-97
Identity = 171/266 (64.29%), Postives = 216/266 (81.20%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           + TR+VW  NL  EF LI++++  YPFIS+DTEFPGV+ +  L      P D Y LLK+N
Sbjct: 17  VVTREVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLRF--TNPDDLYTLLKAN 76

Query: 63  VDALNLIQLGLTLSDADGNLPTLG--TKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI 122
           VDAL+LIQ+GLTLSD +GNLP LG      FIWEFNFRDFDVARD HAPDSIELL++QGI
Sbjct: 77  VDALSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDSIELLRRQGI 136

Query: 123 DFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF 182
           DF RN   GV S RFA+L+MSSGL+CN+ ++WVTFHSAYDFGYL+KILTR +LP  L +F
Sbjct: 137 DFERNCRDGVESERFAELMMSSGLVCNEEVSWVTFHSAYDFGYLMKILTRRELPGALGEF 196

Query: 183 LSILKGLFGKKVYDVKHMMRFCD-GLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAF 242
             +++ LFG++VYDVKHMM+FC+  L+GGLDR+A+TL+++RAVGK HQAGSDSLLT  AF
Sbjct: 197 KRVMRVLFGERVYDVKHMMKFCERRLFGGLDRVARTLEVNRAVGKCHQAGSDSLLTWHAF 256

Query: 243 KKMSDVYFSKDGPDKHAGVLFGLELY 266
           ++M D+YF +DGP+KHAGVL+GLE++
Sbjct: 257 QRMRDLYFVQDGPEKHAGVLYGLEVF 280

BLAST of Chy5G092540 vs. ExPASy Swiss-Prot
Match: Q9LEU4 (Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=CAF1-10 PE=2 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.3e-71
Identity = 133/260 (51.15%), Postives = 186/260 (71.54%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           I  R+VWD NL+ EF LI +++ ++ +I++DTEFPGV+++P +         +Y  LK N
Sbjct: 10  IMIREVWDYNLVEEFALIREIVDKFSYIAMDTEFPGVVLKP-VATFKYNNDLNYRTLKEN 69

Query: 63  VDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDF 122
           VD L LIQ+GLT SD +GNLPT GT    IW+FNFR+F++  D +A +SIELL+Q GIDF
Sbjct: 70  VDLLKLIQVGLTFSDENGNLPTCGTDKFCIWQFNFREFNIGEDIYASESIELLRQCGIDF 129

Query: 123 HRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLS 182
            +N   G+   RF +L+MSSG++ ND+++WVTFH  YDFGYLVK+LT  +LP +  DF  
Sbjct: 130 KKNIEKGIDVVRFGELMMSSGIVLNDAISWVTFHGGYDFGYLVKLLTCKELPLKQADFFK 189

Query: 183 ILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKM 242
           +L   F   VYD+KH+M FC+GL+GGL+RLA+ + ++R VG  HQAGSDSLLTL +F+K+
Sbjct: 190 LLYVYF-PTVYDIKHLMTFCNGLFGGLNRLAELMGVER-VGICHQAGSDSLLTLGSFRKL 249

Query: 243 SDVYFSKDGPDKHAGVLFGL 263
            + YF     +K+ GVL+GL
Sbjct: 250 KERYF-PGSTEKYTGVLYGL 265

BLAST of Chy5G092540 vs. ExPASy Swiss-Prot
Match: Q9SKZ2 (Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=CAF1-7 PE=2 SV=2)

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-70
Identity = 134/261 (51.34%), Postives = 186/261 (71.26%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           IQ R+VW+ NL  E  LI +++  +PF+++DTEFPG++ RP +         HY  LK+N
Sbjct: 10  IQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIVCRP-VGTFKTNTEYHYETLKTN 69

Query: 63  VDALNLIQLGLTLSDADGNLPTLGTKNSF-IWEFNFRDFDVARDPHAPDSIELLKQQGID 122
           V+ L +IQLGLT SD  GNLPT GT N + IW+FNFR+FD+  D +A DSIELL+Q GID
Sbjct: 70  VNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIELLRQSGID 129

Query: 123 FHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFL 182
           F +N   G+ S RFA+LLMSSG++ N+++ WVTFHS YDFGYL+K+LT   LP     F 
Sbjct: 130 FVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTGFF 189

Query: 183 SILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKK 242
            ++   F  +VYD+KH+M+FC+ L+GGL++LA+ LD++R VG  HQAGSDSLLT   F+K
Sbjct: 190 EMISVYF-PRVYDIKHLMKFCNSLHGGLNKLAELLDVER-VGICHQAGSDSLLTSCTFRK 249

Query: 243 MSDVYFSKDGPDKHAGVLFGL 263
           + + +F     +K++GVL+GL
Sbjct: 250 LQENFFI-GSMEKYSGVLYGL 266

BLAST of Chy5G092540 vs. ExPASy Swiss-Prot
Match: Q9SAI2 (Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=CAF1-6 PE=1 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 1.6e-69
Identity = 133/261 (50.96%), Postives = 184/261 (70.50%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           IQ R+VW+ NL  E +LI  ++  +P++++DTEFPG+++RP +         HY  LK+N
Sbjct: 10  IQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRP-VGTFKSNADYHYETLKTN 69

Query: 63  VDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDF 122
           V+ L +IQLGLT S+  GNLPT GT    IW+FNFR+FD+  D  A DSIELLKQ GID 
Sbjct: 70  VNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIELLKQSGIDL 129

Query: 123 HRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLS 182
            +N   G+ S RFA+LLMSSG++ N+++ WVTFHS YDFGYL+K+LT   LP    DF  
Sbjct: 130 AKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFK 189

Query: 183 ILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKM 242
           ++   F   VYD+KH+M+FC+ L+GGL++LA+ L+++R VG  HQAGSDSLLT   F+K+
Sbjct: 190 LINVYF-PTVYDIKHLMKFCNSLHGGLNKLAELLEVER-VGICHQAGSDSLLTSCTFRKL 249

Query: 243 SDVYFSKDGP-DKHAGVLFGL 263
            + +F   GP  K++GVL+GL
Sbjct: 250 KENFFV--GPLHKYSGVLYGL 265

BLAST of Chy5G092540 vs. ExPASy TrEMBL
Match: A0A0A0KI60 (Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_6G425790 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 1.4e-150
Identity = 261/265 (98.49%), Postives = 263/265 (99.25%), Query Frame = 0

Query: 1   MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLK 60
           MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLK
Sbjct: 1   MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLK 60

Query: 61  SNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI 120
           SNVDALNLIQLGLTLSDA GNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI
Sbjct: 61  SNVDALNLIQLGLTLSDAHGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI 120

Query: 121 DFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF 180
           DFHRNRTHGVCSSRFADL+MSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF
Sbjct: 121 DFHRNRTHGVCSSRFADLVMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF 180

Query: 181 LSILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFK 240
           L+ILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLT QAFK
Sbjct: 181 LNILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTFQAFK 240

Query: 241 KMSDVYFSKDGPDKHAGVLFGLELY 266
           KMSDVYFSKDGPDKHAGVLFGLELY
Sbjct: 241 KMSDVYFSKDGPDKHAGVLFGLELY 265

BLAST of Chy5G092540 vs. ExPASy TrEMBL
Match: A0A5D3B887 (Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00210 PE=3 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 1.9e-142
Identity = 248/265 (93.58%), Postives = 255/265 (96.23%), Query Frame = 0

Query: 1   MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLK 60
           MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPL PSDHYLLLK
Sbjct: 1   MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLHPSDHYLLLK 60

Query: 61  SNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI 120
           SNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVAR PH P SIELLKQQGI
Sbjct: 61  SNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARHPHNPASIELLKQQGI 120

Query: 121 DFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF 180
           DFHRNRTHGVCSS+FADLLMSSGLLCN+SLTWVTFHSAYDFGYLVKILTRTKLPSRL DF
Sbjct: 121 DFHRNRTHGVCSSQFADLLMSSGLLCNNSLTWVTFHSAYDFGYLVKILTRTKLPSRLHDF 180

Query: 181 LSILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFK 240
           L+IL  LFG KVYDVKHMMRFCDGLYGGLDR+AKTLDL+RAVGKSHQAGSDSLLT QAFK
Sbjct: 181 LNILGKLFGNKVYDVKHMMRFCDGLYGGLDRVAKTLDLERAVGKSHQAGSDSLLTWQAFK 240

Query: 241 KMSDVYFSKDGPDKHAGVLFGLELY 266
           KM DVYFSKDGP+KHAGVLFGLE+Y
Sbjct: 241 KMRDVYFSKDGPEKHAGVLFGLEIY 265

BLAST of Chy5G092540 vs. ExPASy TrEMBL
Match: A0A1S3BNU2 (Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103491668 PE=3 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.1e-140
Identity = 243/260 (93.46%), Postives = 250/260 (96.15%), Query Frame = 0

Query: 6   RQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSNVDA 65
           RQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPL PSDHYLLLKSNVDA
Sbjct: 1   RQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLHPSDHYLLLKSNVDA 60

Query: 66  LNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDFHRN 125
           LNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVAR PH P SIELLKQQGIDFHRN
Sbjct: 61  LNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARHPHNPASIELLKQQGIDFHRN 120

Query: 126 RTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLSILK 185
           RTHGVCSS+FADLLMSSGLLCN+SLTWVTFHSAYDFGYLVKILTRTKLPSRL DFL+IL 
Sbjct: 121 RTHGVCSSQFADLLMSSGLLCNNSLTWVTFHSAYDFGYLVKILTRTKLPSRLHDFLNILG 180

Query: 186 GLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKMSDV 245
            LFG KVYDVKHMMRFCDGLYGGLDR+AKTLDL+RAVGKSHQAGSDSLLT QAFKKM DV
Sbjct: 181 KLFGNKVYDVKHMMRFCDGLYGGLDRVAKTLDLERAVGKSHQAGSDSLLTWQAFKKMRDV 240

Query: 246 YFSKDGPDKHAGVLFGLELY 266
           YFSKDGP+KHAGVLFGLE+Y
Sbjct: 241 YFSKDGPEKHAGVLFGLEIY 260

BLAST of Chy5G092540 vs. ExPASy TrEMBL
Match: A0A6J1GZY2 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111459188 PE=3 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.4e-118
Identity = 207/263 (78.71%), Postives = 229/263 (87.07%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           I  R+VWD NL HEF LI +L+ RYPFIS+DTEFPGV++RP    HP RPSDHYLLLKSN
Sbjct: 10  IHVREVWDFNLHHEFRLINRLVDRYPFISMDTEFPGVVVRPAF--HPSRPSDHYLLLKSN 69

Query: 63  VDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDF 122
           VDALNLIQ+GLTLSDADGNLPTLGT   FIWEFNFRDFDV RD HAPDSI+LLK+QGIDF
Sbjct: 70  VDALNLIQVGLTLSDADGNLPTLGTDKRFIWEFNFRDFDVTRDLHAPDSIDLLKRQGIDF 129

Query: 123 HRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLS 182
            RNR HGV S+RF++LLMSSGLLCN S++WVTFHSAYDFGYLVKILTR KLPSRL +FL 
Sbjct: 130 ERNRAHGVDSARFSELLMSSGLLCNPSVSWVTFHSAYDFGYLVKILTRRKLPSRLDEFLK 189

Query: 183 ILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKM 242
           ILK  FG KVYDVKHMMRFCD LYGGL+R+AKTL +DRAVGK HQAGSDSLLT  AF+KM
Sbjct: 190 ILKAFFGNKVYDVKHMMRFCDSLYGGLERVAKTLAVDRAVGKCHQAGSDSLLTWHAFQKM 249

Query: 243 SDVYFSKDGPDKHAGVLFGLELY 266
            DVYF KDGP+KHAGVL+GLE+Y
Sbjct: 250 RDVYFGKDGPEKHAGVLYGLEVY 270

BLAST of Chy5G092540 vs. ExPASy TrEMBL
Match: A0A6J1K4S7 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111490755 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.9e-118
Identity = 207/263 (78.71%), Postives = 228/263 (86.69%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           I  R+VWD NL HEF LI  L+ RYPFIS+DTEFPGV++RP    HP RPSDHYLLLKSN
Sbjct: 60  IHVREVWDFNLHHEFRLINHLVDRYPFISMDTEFPGVVVRPAF--HPSRPSDHYLLLKSN 119

Query: 63  VDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDF 122
           VDALNLIQ+GLTLSDADGNLPTLGT   FIWEFNFRDFDV RD HAPDSI+LLK+QGIDF
Sbjct: 120 VDALNLIQVGLTLSDADGNLPTLGTDKRFIWEFNFRDFDVTRDLHAPDSIDLLKRQGIDF 179

Query: 123 HRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLS 182
            RNR HGV S+RF++LLMSSGLLCN S++WVTFHSAYDFGYLVKILTR KLPSRL +FL 
Sbjct: 180 ERNRAHGVDSARFSELLMSSGLLCNPSVSWVTFHSAYDFGYLVKILTRRKLPSRLDEFLK 239

Query: 183 ILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKM 242
           ILK  FG KVYDVKHMMRFCD LYGGL+R+AKTL +DRAVGK HQAGSDSLLT  AF+KM
Sbjct: 240 ILKAFFGNKVYDVKHMMRFCDSLYGGLERVAKTLAVDRAVGKCHQAGSDSLLTWHAFQKM 299

Query: 243 SDVYFSKDGPDKHAGVLFGLELY 266
            DVYF KDGP+KHAGVL+GLE+Y
Sbjct: 300 RDVYFGKDGPEKHAGVLYGLEVY 320

BLAST of Chy5G092540 vs. NCBI nr
Match: XP_004140166.1 (probable CCR4-associated factor 1 homolog 9 [Cucumis sativus] >KGN48042.1 hypothetical protein Csa_004151 [Cucumis sativus])

HSP 1 Score: 538 bits (1385), Expect = 7.43e-193
Identity = 261/265 (98.49%), Postives = 263/265 (99.25%), Query Frame = 0

Query: 1   MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLK 60
           MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLK
Sbjct: 1   MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLK 60

Query: 61  SNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI 120
           SNVDALNLIQLGLTLSDA GNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI
Sbjct: 61  SNVDALNLIQLGLTLSDAHGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI 120

Query: 121 DFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF 180
           DFHRNRTHGVCSSRFADL+MSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF
Sbjct: 121 DFHRNRTHGVCSSRFADLVMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF 180

Query: 181 LSILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFK 240
           L+ILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLT QAFK
Sbjct: 181 LNILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTFQAFK 240

Query: 241 KMSDVYFSKDGPDKHAGVLFGLELY 265
           KMSDVYFSKDGPDKHAGVLFGLELY
Sbjct: 241 KMSDVYFSKDGPDKHAGVLFGLELY 265

BLAST of Chy5G092540 vs. NCBI nr
Match: KAA0061726.1 (putative CCR4-associated factor 1-like protein 11 [Cucumis melo var. makuwa] >TYJ96062.1 putative CCR4-associated factor 1-like protein 11 [Cucumis melo var. makuwa])

HSP 1 Score: 511 bits (1315), Expect = 3.49e-182
Identity = 248/265 (93.58%), Postives = 255/265 (96.23%), Query Frame = 0

Query: 1   MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLK 60
           MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPL PSDHYLLLK
Sbjct: 1   MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLHPSDHYLLLK 60

Query: 61  SNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI 120
           SNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVAR PH P SIELLKQQGI
Sbjct: 61  SNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARHPHNPASIELLKQQGI 120

Query: 121 DFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF 180
           DFHRNRTHGVCSS+FADLLMSSGLLCN+SLTWVTFHSAYDFGYLVKILTRTKLPSRL DF
Sbjct: 121 DFHRNRTHGVCSSQFADLLMSSGLLCNNSLTWVTFHSAYDFGYLVKILTRTKLPSRLHDF 180

Query: 181 LSILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFK 240
           L+IL  LFG KVYDVKHMMRFCDGLYGGLDR+AKTLDL+RAVGKSHQAGSDSLLT QAFK
Sbjct: 181 LNILGKLFGNKVYDVKHMMRFCDGLYGGLDRVAKTLDLERAVGKSHQAGSDSLLTWQAFK 240

Query: 241 KMSDVYFSKDGPDKHAGVLFGLELY 265
           KM DVYFSKDGP+KHAGVLFGLE+Y
Sbjct: 241 KMRDVYFSKDGPEKHAGVLFGLEIY 265

BLAST of Chy5G092540 vs. NCBI nr
Match: XP_008449941.1 (PREDICTED: probable CCR4-associated factor 1 homolog 11, partial [Cucumis melo])

HSP 1 Score: 503 bits (1295), Expect = 3.25e-179
Identity = 243/260 (93.46%), Postives = 250/260 (96.15%), Query Frame = 0

Query: 6   RQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSNVDA 65
           RQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPL PSDHYLLLKSNVDA
Sbjct: 1   RQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLHPSDHYLLLKSNVDA 60

Query: 66  LNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDFHRN 125
           LNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVAR PH P SIELLKQQGIDFHRN
Sbjct: 61  LNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARHPHNPASIELLKQQGIDFHRN 120

Query: 126 RTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLSILK 185
           RTHGVCSS+FADLLMSSGLLCN+SLTWVTFHSAYDFGYLVKILTRTKLPSRL DFL+IL 
Sbjct: 121 RTHGVCSSQFADLLMSSGLLCNNSLTWVTFHSAYDFGYLVKILTRTKLPSRLHDFLNILG 180

Query: 186 GLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKMSDV 245
            LFG KVYDVKHMMRFCDGLYGGLDR+AKTLDL+RAVGKSHQAGSDSLLT QAFKKM DV
Sbjct: 181 KLFGNKVYDVKHMMRFCDGLYGGLDRVAKTLDLERAVGKSHQAGSDSLLTWQAFKKMRDV 240

Query: 246 YFSKDGPDKHAGVLFGLELY 265
           YFSKDGP+KHAGVLFGLE+Y
Sbjct: 241 YFSKDGPEKHAGVLFGLEIY 260

BLAST of Chy5G092540 vs. NCBI nr
Match: XP_038901771.1 (probable CCR4-associated factor 1 homolog 11 [Benincasa hispida])

HSP 1 Score: 465 bits (1196), Expect = 4.79e-164
Identity = 225/265 (84.91%), Postives = 241/265 (90.94%), Query Frame = 0

Query: 1   MLIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLK 60
           M IQ RQVWD NL +EFNLI  L HR+PFIS+DTEFPGV+IRP LHR  LRPSDHYLLLK
Sbjct: 1   MFIQIRQVWDFNLDYEFNLINHLFHRFPFISMDTEFPGVVIRPALHRPRLRPSDHYLLLK 60

Query: 61  SNVDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI 120
           SNVDALNLIQ+GLTLSDADGNLPTLGT N FIWEFNFRDFDV RD HAPDSIELLK+QGI
Sbjct: 61  SNVDALNLIQVGLTLSDADGNLPTLGTDNCFIWEFNFRDFDVVRDLHAPDSIELLKRQGI 120

Query: 121 DFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF 180
           DF RNR HGV SSRFA+LLMSSGLLCNDS+TWVTFHSAYDFGYLVKILTRTKLP+RL DF
Sbjct: 121 DFERNRIHGVPSSRFAELLMSSGLLCNDSVTWVTFHSAYDFGYLVKILTRTKLPARLHDF 180

Query: 181 LSILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFK 240
           L IL+GLFG KVYDVKHMMRFCDGLYGGLDR+AKTL +DRAVGKSHQAGSDSLLT QAF+
Sbjct: 181 LKILRGLFGNKVYDVKHMMRFCDGLYGGLDRVAKTLAVDRAVGKSHQAGSDSLLTWQAFQ 240

Query: 241 KMSDVYFSKDGPDKHAGVLFGLELY 265
           KM DVYF KDGP+KHAGVL+GLE+Y
Sbjct: 241 KMRDVYFFKDGPEKHAGVLYGLEVY 265

BLAST of Chy5G092540 vs. NCBI nr
Match: XP_022957732.1 (probable CCR4-associated factor 1 homolog 11 [Cucurbita moschata] >KAG6605970.1 putative CCR4-associated factor 1-like 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 431 bits (1109), Expect = 1.03e-150
Identity = 207/263 (78.71%), Postives = 229/263 (87.07%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           I  R+VWD NL HEF LI +L+ RYPFIS+DTEFPGV++RP  H  P RPSDHYLLLKSN
Sbjct: 10  IHVREVWDFNLHHEFRLINRLVDRYPFISMDTEFPGVVVRPAFH--PSRPSDHYLLLKSN 69

Query: 63  VDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDF 122
           VDALNLIQ+GLTLSDADGNLPTLGT   FIWEFNFRDFDV RD HAPDSI+LLK+QGIDF
Sbjct: 70  VDALNLIQVGLTLSDADGNLPTLGTDKRFIWEFNFRDFDVTRDLHAPDSIDLLKRQGIDF 129

Query: 123 HRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLS 182
            RNR HGV S+RF++LLMSSGLLCN S++WVTFHSAYDFGYLVKILTR KLPSRL +FL 
Sbjct: 130 ERNRAHGVDSARFSELLMSSGLLCNPSVSWVTFHSAYDFGYLVKILTRRKLPSRLDEFLK 189

Query: 183 ILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKM 242
           ILK  FG KVYDVKHMMRFCD LYGGL+R+AKTL +DRAVGK HQAGSDSLLT  AF+KM
Sbjct: 190 ILKAFFGNKVYDVKHMMRFCDSLYGGLERVAKTLAVDRAVGKCHQAGSDSLLTWHAFQKM 249

Query: 243 SDVYFSKDGPDKHAGVLFGLELY 265
            DVYF KDGP+KHAGVL+GLE+Y
Sbjct: 250 RDVYFGKDGPEKHAGVLYGLEVY 270

BLAST of Chy5G092540 vs. TAIR 10
Match: AT5G22250.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 356.7 bits (914), Expect = 1.6e-98
Identity = 175/268 (65.30%), Postives = 218/268 (81.34%), Query Frame = 0

Query: 2   LIQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLH-RHPLRPSDHYLLLK 61
           +I  R VW  NL  EF+LI  ++  YPFIS+DTEFPGV+ +  L       P+  Y LLK
Sbjct: 11  VIVIRDVWAYNLESEFDLIRGIVEDYPFISMDTEFPGVIYKADLDVLRRGNPNYLYNLLK 70

Query: 62  SNVDALNLIQLGLTLSDADGNLPTLGTKNS--FIWEFNFRDFDVARDPHAPDSIELLKQQ 121
           SNVDAL+LIQ+GLTLSDADGNLP LG + +  +IWEFNFRDFDV RDPHAPDSIELL++ 
Sbjct: 71  SNVDALSLIQVGLTLSDADGNLPDLGGQKNRRYIWEFNFRDFDVERDPHAPDSIELLRRH 130

Query: 122 GIDFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQ 181
           GIDF RNR  GV S RFA+L+MSSGL+CN+S++WVTFHSAYDFGYLVKILTR +LP  L+
Sbjct: 131 GIDFERNRREGVESERFAELMMSSGLICNESVSWVTFHSAYDFGYLVKILTRRQLPVALR 190

Query: 182 DFLSILKGLFGKKVYDVKHMMRFCD-GLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQ 241
           +FL +L+  FG +VYDVKH+MRFC+  LYGGLDR+A++L+++RAVGK HQAGSDSLLT Q
Sbjct: 191 EFLGLLRAFFGDRVYDVKHIMRFCEQRLYGGLDRVARSLEVNRAVGKCHQAGSDSLLTWQ 250

Query: 242 AFKKMSDVYFSKDGPDKHAGVLFGLELY 266
           AF++M D+YF +DG +KHAGVL+GLE++
Sbjct: 251 AFQRMRDLYFVEDGAEKHAGVLYGLEVF 278

BLAST of Chy5G092540 vs. TAIR 10
Match: AT3G44260.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 355.5 bits (911), Expect = 3.6e-98
Identity = 171/266 (64.29%), Postives = 216/266 (81.20%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           + TR+VW  NL  EF LI++++  YPFIS+DTEFPGV+ +  L      P D Y LLK+N
Sbjct: 17  VVTREVWAENLESEFELISEIIDDYPFISMDTEFPGVIFKSDLRF--TNPDDLYTLLKAN 76

Query: 63  VDALNLIQLGLTLSDADGNLPTLG--TKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGI 122
           VDAL+LIQ+GLTLSD +GNLP LG      FIWEFNFRDFDVARD HAPDSIELL++QGI
Sbjct: 77  VDALSLIQVGLTLSDVNGNLPDLGDDLHRGFIWEFNFRDFDVARDAHAPDSIELLRRQGI 136

Query: 123 DFHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDF 182
           DF RN   GV S RFA+L+MSSGL+CN+ ++WVTFHSAYDFGYL+KILTR +LP  L +F
Sbjct: 137 DFERNCRDGVESERFAELMMSSGLVCNEEVSWVTFHSAYDFGYLMKILTRRELPGALGEF 196

Query: 183 LSILKGLFGKKVYDVKHMMRFCD-GLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAF 242
             +++ LFG++VYDVKHMM+FC+  L+GGLDR+A+TL+++RAVGK HQAGSDSLLT  AF
Sbjct: 197 KRVMRVLFGERVYDVKHMMKFCERRLFGGLDRVARTLEVNRAVGKCHQAGSDSLLTWHAF 256

Query: 243 KKMSDVYFSKDGPDKHAGVLFGLELY 266
           ++M D+YF +DGP+KHAGVL+GLE++
Sbjct: 257 QRMRDLYFVQDGPEKHAGVLYGLEVF 280

BLAST of Chy5G092540 vs. TAIR 10
Match: AT5G10960.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 9.0e-73
Identity = 133/260 (51.15%), Postives = 186/260 (71.54%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           I  R+VWD NL+ EF LI +++ ++ +I++DTEFPGV+++P +         +Y  LK N
Sbjct: 10  IMIREVWDYNLVEEFALIREIVDKFSYIAMDTEFPGVVLKP-VATFKYNNDLNYRTLKEN 69

Query: 63  VDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDF 122
           VD L LIQ+GLT SD +GNLPT GT    IW+FNFR+F++  D +A +SIELL+Q GIDF
Sbjct: 70  VDLLKLIQVGLTFSDENGNLPTCGTDKFCIWQFNFREFNIGEDIYASESIELLRQCGIDF 129

Query: 123 HRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLS 182
            +N   G+   RF +L+MSSG++ ND+++WVTFH  YDFGYLVK+LT  +LP +  DF  
Sbjct: 130 KKNIEKGIDVVRFGELMMSSGIVLNDAISWVTFHGGYDFGYLVKLLTCKELPLKQADFFK 189

Query: 183 ILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKM 242
           +L   F   VYD+KH+M FC+GL+GGL+RLA+ + ++R VG  HQAGSDSLLTL +F+K+
Sbjct: 190 LLYVYF-PTVYDIKHLMTFCNGLFGGLNRLAELMGVER-VGICHQAGSDSLLTLGSFRKL 249

Query: 243 SDVYFSKDGPDKHAGVLFGL 263
            + YF     +K+ GVL+GL
Sbjct: 250 KERYF-PGSTEKYTGVLYGL 265

BLAST of Chy5G092540 vs. TAIR 10
Match: AT2G32070.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 268.1 bits (684), Expect = 7.7e-72
Identity = 134/261 (51.34%), Postives = 186/261 (71.26%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           IQ R+VW+ NL  E  LI +++  +PF+++DTEFPG++ RP +         HY  LK+N
Sbjct: 10  IQIREVWNDNLESEMALIREVVDDFPFVAMDTEFPGIVCRP-VGTFKTNTEYHYETLKTN 69

Query: 63  VDALNLIQLGLTLSDADGNLPTLGTKNSF-IWEFNFRDFDVARDPHAPDSIELLKQQGID 122
           V+ L +IQLGLT SD  GNLPT GT N + IW+FNFR+FD+  D +A DSIELL+Q GID
Sbjct: 70  VNILKMIQLGLTFSDEKGNLPTCGTDNKYCIWQFNFREFDLESDIYATDSIELLRQSGID 129

Query: 123 FHRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFL 182
           F +N   G+ S RFA+LLMSSG++ N+++ WVTFHS YDFGYL+K+LT   LP     F 
Sbjct: 130 FVKNNEFGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPETQTGFF 189

Query: 183 SILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKK 242
            ++   F  +VYD+KH+M+FC+ L+GGL++LA+ LD++R VG  HQAGSDSLLT   F+K
Sbjct: 190 EMISVYF-PRVYDIKHLMKFCNSLHGGLNKLAELLDVER-VGICHQAGSDSLLTSCTFRK 249

Query: 243 MSDVYFSKDGPDKHAGVLFGL 263
           + + +F     +K++GVL+GL
Sbjct: 250 LQENFFI-GSMEKYSGVLYGL 266

BLAST of Chy5G092540 vs. TAIR 10
Match: AT1G80780.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 264.2 bits (674), Expect = 1.1e-70
Identity = 133/261 (50.96%), Postives = 184/261 (70.50%), Query Frame = 0

Query: 3   IQTRQVWDLNLLHEFNLITQLLHRYPFISIDTEFPGVLIRPTLHRHPLRPSDHYLLLKSN 62
           IQ R+VW+ NL  E +LI  ++  +P++++DTEFPG+++RP +         HY  LK+N
Sbjct: 10  IQIREVWNDNLQEEMDLIRDVVDDFPYVAMDTEFPGIVVRP-VGTFKSNADYHYETLKTN 69

Query: 63  VDALNLIQLGLTLSDADGNLPTLGTKNSFIWEFNFRDFDVARDPHAPDSIELLKQQGIDF 122
           V+ L +IQLGLT S+  GNLPT GT    IW+FNFR+FD+  D  A DSIELLKQ GID 
Sbjct: 70  VNILKMIQLGLTFSNEQGNLPTCGTDKYCIWQFNFREFDLDSDIFALDSIELLKQSGIDL 129

Query: 123 HRNRTHGVCSSRFADLLMSSGLLCNDSLTWVTFHSAYDFGYLVKILTRTKLPSRLQDFLS 182
            +N   G+ S RFA+LLMSSG++ N+++ WVTFHS YDFGYL+K+LT   LP    DF  
Sbjct: 130 AKNTLDGIDSKRFAELLMSSGIVLNENVHWVTFHSGYDFGYLLKLLTCQNLPDSQTDFFK 189

Query: 183 ILKGLFGKKVYDVKHMMRFCDGLYGGLDRLAKTLDLDRAVGKSHQAGSDSLLTLQAFKKM 242
           ++   F   VYD+KH+M+FC+ L+GGL++LA+ L+++R VG  HQAGSDSLLT   F+K+
Sbjct: 190 LINVYF-PTVYDIKHLMKFCNSLHGGLNKLAELLEVER-VGICHQAGSDSLLTSCTFRKL 249

Query: 243 SDVYFSKDGP-DKHAGVLFGL 263
            + +F   GP  K++GVL+GL
Sbjct: 250 KENFFV--GPLHKYSGVLYGL 265

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMS62.3e-9765.30Probable CCR4-associated factor 1 homolog 11 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LXM25.1e-9764.29Probable CCR4-associated factor 1 homolog 9 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9LEU41.3e-7151.15Probable CCR4-associated factor 1 homolog 10 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9SKZ21.1e-7051.34Probable CCR4-associated factor 1 homolog 7 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Q9SAI21.6e-6950.96Probable CCR4-associated factor 1 homolog 6 OS=Arabidopsis thaliana OX=3702 GN=C... [more]
Match NameE-valueIdentityDescription
A0A0A0KI601.4e-15098.49Poly(A)-specific ribonuclease OS=Cucumis sativus OX=3659 GN=Csa_6G425790 PE=3 SV... [more]
A0A5D3B8871.9e-14293.58Poly(A)-specific ribonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A1S3BNU25.1e-14093.46Poly(A)-specific ribonuclease OS=Cucumis melo OX=3656 GN=LOC103491668 PE=3 SV=1[more]
A0A6J1GZY21.4e-11878.71Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111459188 PE=3... [more]
A0A6J1K4S71.9e-11878.71Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111490755 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_004140166.17.43e-19398.49probable CCR4-associated factor 1 homolog 9 [Cucumis sativus] >KGN48042.1 hypoth... [more]
KAA0061726.13.49e-18293.58putative CCR4-associated factor 1-like protein 11 [Cucumis melo var. makuwa] >TY... [more]
XP_008449941.13.25e-17993.46PREDICTED: probable CCR4-associated factor 1 homolog 11, partial [Cucumis melo][more]
XP_038901771.14.79e-16484.91probable CCR4-associated factor 1 homolog 11 [Benincasa hispida][more]
XP_022957732.11.03e-15078.71probable CCR4-associated factor 1 homolog 11 [Cucurbita moschata] >KAG6605970.1 ... [more]
Match NameE-valueIdentityDescription
AT5G22250.11.6e-9865.30Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT3G44260.13.6e-9864.29Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT5G10960.19.0e-7351.15Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT2G32070.17.7e-7251.34Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
AT1G80780.11.1e-7050.96Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 1..264
e-value: 1.1E-90
score: 305.4
IPR006941Ribonuclease CAF1PFAMPF04857CAF1coord: 11..130
e-value: 5.3E-8
score: 32.5
NoneNo IPR availablePANTHERPTHR10797:SF48CCR4-ASSOCIATED FACTOR 1 HOMOLOG 11-RELATEDcoord: 4..265
IPR039637CCR4-NOT transcription complex subunit 7/8/Pop2PANTHERPTHR10797CCR4-NOT TRANSCRIPTION COMPLEX SUBUNITcoord: 4..265
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 4..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G092540.1Chy5G092540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043928 exonucleolytic catabolism of deadenylated mRNA
biological_process GO:0090503 RNA phosphodiester bond hydrolysis, exonucleolytic
cellular_component GO:0030015 CCR4-NOT core complex
cellular_component GO:0005634 nucleus
cellular_component GO:0000932 P-body
cellular_component GO:0030014 CCR4-NOT complex
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004535 poly(A)-specific ribonuclease activity
molecular_function GO:0016740 transferase activity