Chy5G091400 (gene) Cucumber (hystrix) v1

Overview
NameChy5G091400
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionDNA topoisomerase 6 subunit B
LocationchrH05: 639374 .. 646223 (-)
RNA-Seq ExpressionChy5G091400
SyntenyChy5G091400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGCAGGAGGTGGCAGTGAAAGTCCTGATGAGCCGAAGAAGGGCAAATCAAAAACACCCAGGAAGCCCAAAGAAAGCATCCTCAAGCAAAGTATCTTCTCCCTCTTTTACGAGTTCGATTTCTTTGTTGTTTTTCTACTTTTACTAATTTGATTGAGTTTTGTCTCACTTTGCAGAATCACCAGCTGAGTTCTTTGCAGAAAACAAGAATATTGCTGGGTTTGATAATGTGAGCTGCATTCAGTTTTTTCTCTCTCTCTCTGTTCATTTCTTACGGGTTAGTTTTAGTGCTAGGAATCGCCGGAATTAACCAAGGTGAATTGTGGTTGTTTTTCTTGATATAATTGAATTGGGAAGAAGTTAGGATGCTAGTTAAGAGTATTTTCGTACGCGAGTTTTGTTGTTATTGTTATTGATACTTCGAGATCTTCTATGAATTGCCAGCCTGGGAAGTGTCTCTATACTACTGTGAGGGAGCTTGTGGAAAATTCTCTTGATTCTGCAGAGTCTATATCTGAGCTTCCCGTGGTAGAAATTACTATGTAAGTTTACGTTCGCATGAACATAACTCAAATTTTATTTTAGAAAATTCCACATAAATGGTCGACTATGCAAATCAAATTGGTTAAGATATGTGAGTTCCATGGATGTTTTGCAATGCAGTGAAGAGATATTGAAAACCAAGTTTAATTCTATGATTGGTCTGGTTGATCGAGAACGTGTTGATGAGGAACTTTATGATGATTATGAGACTGCTAAGGCTCGTGAGGTTTGTTAATCTTAAATTACCCAGGTCTTCACCAAATCAGATGTAGAAAGATATTTCTGATAAAATTCGTACTGTGCATTTCATTTTTTTTTTTTTTTTTGTCTCAGAAAAGATTAGCGAAAGAAGCACGTGCTCAAGAAATACAGGCGAAAAATGCTGCCCTTGGGAAGAAAGGCAGAGAACCTGCAGCCTCTAAGGGTATGAAAGGTCGTGGTGAGGCTTCATTCTACCGGGTGACTTGCAAAGTACGATTTTTCTCCATTCCCGCATCCTTTGGTCAATCATGCAAAGCATACCTTTTTGTTCCCTTAAAATTTTTCTTCTTCTGCAGATATAGATCTTGAATATCTTATGTTGTAATCAAACTTAGTTCTTTTTCTTTTTTTTCTTTTTTTAAATCTCAAGGATAATGGAAAAGGAATGCCCCATGATGACATACCAAATATGTTTGGCCGAGGTTAATATATTACGTTGATGGTTTACTTCCTTCTCTCACCTGTGTTTATGCCAATGAGATCGAATAGTTACTTCAAACTAATAAAACCTATGCTGCTCGATAGTATTTCATAAAACTCTCAGTATTTTGTGTGCAGTGCTCTCCGGAACAAAATATGGCTTGAAGCAAACCCGTGGAAAGTTTGGTCTGGGTGCAAAGATGGTAGGCACTGTTCTATATACTGAGGAAATGACTCTATTCGACTGAATGCTTTATAACACAGTTGCATATTCACTTGCACTTGGAAAGTATTGTAATTACTATGATTTGCATACTAAATTCTAATTCCATGTTAGCTTTCATGCATAGGGGTTTAACTAAGGCAAGTTGAATTAATATGAGACACCATTGTCATTATTTAACATAGCCTTATGCCTAAACGAAGTAGCTTCACATATACTGTATGGGTTCAACCAGAATGTTATGTCATATGTGTAACTCTTAATAGCAATTAGTTCTCATTTTTAGTTATGTAAATGACTACGTATTATATTATGTGTACGAAATGCAATCAATCTATTACCTTTGTTTTTCCACTCGTTAAGATGGGACACATTTTTAAATTCCTATAATTTTGCAGGCATTAATTTGGTCTAAGATGAGTACTGGTCTTCCTATAGAGATATCATCATCAATGAAGGGTCAAAACTATACTTCATTCTGCCGCTTAGATATTGATATTCATCGGTATGCTAGGCTTTTTGGATGCATTGATGATTTTATGATATATTTTTCTTGCATACTTGATTTTGTTCTTCTCATTAGCACACAATAAATCATATATTCCCTTAAATTTTTTTTTTTTTAATAAGATTGTTTGTCCATTTTAACAATATCTGCTTGGCTTTCATGTAATTTCCTTTTTGGTCATTGGTTCCTGTTGACCTCATTTTATGTGCATCTCCCTACAGGAACATTCCACACATTCATATCCAAGAAAAACGTGATAACAAGGATCTATGGCATGGGGCTGAAATTCAAGTTGTAATTGAAGGAAATTGGACAACTTACCGTGTATGCTGTACTTTAATGATACTTACTATTTCCCATTTTGAGGGAACTTCCAGTCCCTAGAGTTGTTGAGTAAATATTTCACATCTTTAATACATTCCATATTGTGCAAATGCAGTCGAAAATTTTGCATTATATGCGACAGATGGCAGTTATTACACCTTATGCTCAATTTCTTTTCAGATTTGTTTCAGACACACCTGAGTATGTTCTCTCTCTCTCTTTCTCACGTGCACATCCCAAAAACTATTGTGTATCTTATATGCCCTTTTTATGCAGTAAAAATATCACAATTAGATTTTCTCGAAGAACCGATGTTATGCCTCCAGTTCCTCTAGAGACTAAACACCATCCATCTGCTGTTGATTTACTGTTGATAAAGCGACTTATTGCAGAAACAGCTAAACAGAACCTTTTACAGTTTCTCCAGCATGAATTTGTAAACATAGGGAAGCCATTTGCTGAACGGTTAATTGGTTAGCATATCATCCCTTTCTTAATCATCGTGCCTATTGTCTACTTACAGAAACTTTTATTTTTGCAAGAGATTTTCTACTTCTTAATTTCTTCTCCCAGTACCTTTTTCTGTGGGTTTAGAAACACTTACACACATCAAAAGGAAAAAGAGAGTTAAAAAAAAACAGAAAAATGAAAACATTTATGACATAATAATGAATAGATAAATGATGGAAACCAGAAACTATAGAGAAATTTTACTTTTATTGATATTAGATTCAACAATGAATCTGTAGTACAAACACTAATTTGTAAACAAATGAAAACTCAAACCCTCCTCTGTTATCCTATCTCTCAAGGAAGAACAGAAAACTTTTCTTCAAAAAACTCTAACTGCCTCTCCCGCCCAATCCTTATCCTTTATTTATACTAACCTAACTCACACGCGTCACTCTACACACGTGCCACTACTCTTCTTCCCTTTCCTACTATATTGATATAGTACCGGGGGTCTATCAATAAAGTTCTCCAATACTACTCCTTGTTATCAAAGATGGCTTGTGTTTATAGTTATGTTACTTGGGGTCCTGGGAGGTGTTTACTTTCACGAGTGCCTGGCCGTGCCTGAACAACGCTGCACGGGGCTAAAAATCTGTTAAGCTCTTAAGTAATTCTTTTGATTGGTATTGCTAGAGGCTGTTTTAATATTATCTTAAATTAGTTGCTTGTGATGATTTATTTTTAGATTTTTAGTTCCAGTTGATTACATTCTTAATAAGTAAAATTAGAAGTTGATTGCATATTCTCTTATGAATAATCTTTCCCAGGCGAAATGGGCCCAGAATTTAGCCCAAAAATGTCTGTTAAGTCTCTGACATCTCAGCAAATAGTCCGTATTCATCAGTTGTTTCGTCAAGCTAAATTTGATGATCCTAGTGGTGATGTAAGTTGCCTCATTCTTTTTGTTAACTATGAGCCTTGGTTCTTTGAATCTCTTCTTGGTTTCTGTATATAAGTTTGTACGAATTATTTGTGCACATTTTTGCAGTGTCTTAGTCCTGCTGGGGAGTACAATCTTCGACTTGGAATTATAAAGGAACTGCATCCTGACATGGTTGCTACTTATTCAGGCAGGTTGTACTTGAATTTGGAACTCTGCAAATTGTGCTAGATCTGCATGCTTCTTATTCTTACTTGAAGCTGATATTGATATGATGTGCGTATATATTCCTTCTAAATAAACTAGAGGCCTTGATTTTTTGCCTTGGTAATTCATATTAGCTTCCAAGTATGTTAATCTTATGTCAAATGTTGACAGTGCTCAAGTTTTCGAGGGACATCCATTCATTGTTGAAGCTGGTGTTAGTATTGGAGGAAAAGATGTGAAACAGGTTTCTTCCTAATTGGTTTAACTTATTTTATTTGCCATTTAAGTACTATGCTAGCTTGCGTTATTTTCAGCATTCTATTCGATCTAATTTTAGGGTGGTGCTAGTGCTAGTCTGCCCGAGGAAGTGAAGAGACAAAAATGATGCCCTCCCTTAATATCAGTCTTCCTGCATGTACATTGACTGAAATTTAACGATGATAGACATTTGAACGTCTTATTTTAATTCCAATTACTTATAAAATAAAGGTTAAATTACAAAAAAAAAAAAAAAAAAAAAAAAGCCCCTAAACTTTCAATAGTTTTAAAAATACCCTTAAACTTTCGAAAAGGGTTAAAAAATGTCTTTACCGTTACTTTTGGATGGAAACCGATAAAGTTTTGTTTAAAAAATACTCCTAAACTATTGAAAAGTTTCATAAATACCTTTGAACTAAAAAAAAAAAAAAACTCTCATAAATCCAATCTTACGCCAAATTTCTTAACACCCAAAATAAAAGCAAAAAATTTAAGTTAAAACTATAGTTTTAAATTTCCATCAATATGTCGATATTTTCATAGTTCCATGGGTTCGTTAATGATATTTTCATTACATCTTAAAAAAATATTTAAAAAATCCCTGAAATACAAAAAATAAACATCAAAATGACACTGATATAAATATAATATAAATAATAGACATGTTTACTTATTTAAAAATAATAAAATATTTATTTAATTGTTCAAATTTATTTTTTGATTTTTTTTGTAATTTTTCCAGAAATATCCATTAAAATTATAATTTCTGTAAAATTAAGACTTTGATATATCCATTAAACATATTAAAATAATAATTAGTCAAACAAAAAAAAGTCTCACACGGATAGATCTATCATAGCAGCAACCCACAAAGTCATAACTAGTGTCATATCTCTTAGTTTTCATTTGCTAGCTTTATATACTCTATATTTTGAGTCCACGCAAGTAAAATACTTCGATAAAACTTTATTTGATTGTGAAAAAGTAAATGTAAATAAATATTGGAACTTGTTCTTATTACAGGGTCTGAATATTTTCCGGTTTGCTAATCGGATACCCCTTCTTTTTGAGCAAGGTGCTGATGTCGTTACTAGGACTGCACTTAAGAGGATCAAGTATGAACGGAAACATTTGATATTTCCATTTAAATTCTTTCTTTTTTTGATAGCGGAATGAAGATTGTTGGCGGAATCTTGTTTTGGCCAATCTTGTAAATTGAAGTTCTAGTTTTTTAACAGAAGACTGACTTCCATCTTAACCATTTTACCTCTCTTCCTTTCTCCTTCTTTAGTTGGAATAGTTATAAAATCAATCAGACTCAAGACAAAATTGGTGTGTTCGTGAGTATTGTTAGTACTAAGATTCCTTTCAAGGGAACTGGAAAGGAGTACATTGGAGTTGACATATCTGAAATAGCATCTGCTGTCAAGGTATACATTTTCATTATAACTACAATATGCATTTGTTAAGAACCGTCACCATTAACTTTATGCTGACGTTGTTAGTTTTACAGTCTGCCATTCAGCAGTGCTGCATTCAATTGAAAAGCAAAATAGTGAAGAAAATACAGGCACGCGAGCAACAGGAGAGGAAACGGAATTTGAGCAGGTAAATTTTACATTTCCTCTTTTGAATGATTGATATAATTAAATTTATTATCTAAGTTTTTTGGTTGATTATAGGTGATTTGAAATATACCTTTCTTGGCCTTTTGGCTAAGATCAAATGTAGGTGATTTAAAATGGAATTAAAACAGGAGGTCATGTGTTCGACCTCTTGTGGAGGAAGGGTTGAAGTATTATTTTACTATAACTCATCAACTTAAGCTTTTGGATTGATTGTTGGTTTAACAATCTCAACTCATGCCTCTTGGATTTCTTTCTTCATAATGTTCTACCATTTTGAAGATTTTGCCGTTGTATATATTTTCCTTGAGCTAATATATTCACATTGTATTGACTTGGTTTATAGATATATCCCGGATGCAACAAATGCTGTTTATAATGTTTTGAAAGAAATGGCTCAAGTGCATGCATCAAAGAAGAAACGTCATGACTGTGAGGATGCTGAAATACTCAGTAAAGTATCTGCTCGCTTGATTACAAATGAGACATTGAGGGATAAACTTGCACAACATGTTGAACAGGTGAGGTCAAGCCATATTCTGCTTGTGCCTAATTTTAAGTTATAAACTGATCCGTGGAGTATTAATTTAACTTCAACGCTAGTTTGCTGAGAAATTATTCAAGGAAGCTACAGGGTGGTGACCGTTGGGTGATCCAATACAATTACAGAAACTAAAGATTTCGTTTAACCATACATCCAAGGGTGGTTTAGTGGGGAGTAGCACATAGTTTTTTCAGTTTGAGACATCTGTTTAAACCATCATAGCCAAAAAGATAAAAAAAGTCTGGTTCTGTTTGGTTATCTATGGAATCTGCTTGGTTTTGGACTGAAAACAGACCAAAGTGGTTTACGATTCTATCTTAGCAAAACTATAGTTTGGCCTAGTGGAATCGAGTTTACTACATATAACAAATTTCCATTTCATTTCATTTTTCAGGTCGACCACGAAATGGCCATGGAATATGCCACACAGAGTGGAGCAAGTGCAGAAGCCAAAGAAGAAATGTATATTCAATCACTGGATGCAACACATAACTGCACTGACTTCCAGAGCCCTTTGTTTGTTTTCAGACTGATTTGTTAA

mRNA sequence

ATGGAGGCAGGAGGTGGCAGTGAAAGTCCTGATGAGCCGAAGAAGGGCAAATCAAAAACACCCAGGAAGCCCAAAGAAAGCATCCTCAAGCAAAAATCACCAGCTGAGTTCTTTGCAGAAAACAAGAATATTGCTGGGTTTGATAATCCTGGGAAGTGTCTCTATACTACTGTGAGGGAGCTTGTGGAAAATTCTCTTGATTCTGCAGAGTCTATATCTGAGCTTCCCGTGGTAGAAATTACTATTGAAGAGATATTGAAAACCAAGTTTAATTCTATGATTGGTCTGGTTGATCGAGAACGTGTTGATGAGGAACTTTATGATGATTATGAGACTGCTAAGGCTCGTGAGAAAAGATTAGCGAAAGAAGCACGTGCTCAAGAAATACAGGCGAAAAATGCTGCCCTTGGGAAGAAAGGCAGAGAACCTGCAGCCTCTAAGGGTATGAAAGGTCGTGGTGAGGCTTCATTCTACCGGGTGACTTGCAAAGATAATGGAAAAGGAATGCCCCATGATGACATACCAAATATGTTTGGCCGAGTGCTCTCCGGAACAAAATATGGCTTGAAGCAAACCCGTGGAAAGTTTGGTCTGGGTGCAAAGATGGCATTAATTTGGTCTAAGATGAGTACTGGTCTTCCTATAGAGATATCATCATCAATGAAGGGTCAAAACTATACTTCATTCTGCCGCTTAGATATTGATATTCATCGGAACATTCCACACATTCATATCCAAGAAAAACGTGATAACAAGGATCTATGGCATGGGGCTGAAATTCAAGTTGTAATTGAAGGAAATTGGACAACTTACCGTTCGAAAATTTTGCATTATATGCGACAGATGGCAGTTATTACACCTTATGCTCAATTTCTTTTCAGATTTGTTTCAGACACACCTGATAAAAATATCACAATTAGATTTTCTCGAAGAACCGATGTTATGCCTCCAGTTCCTCTAGAGACTAAACACCATCCATCTGCTGTTGATTTACTGTTGATAAAGCGACTTATTGCAGAAACAGCTAAACAGAACCTTTTACAGTTTCTCCAGCATGAATTTGTAAACATAGGGAAGCCATTTGCTGAACGGTTAATTGGCGAAATGGGCCCAGAATTTAGCCCAAAAATGTCTGTTAAGTCTCTGACATCTCAGCAAATAGTCCGTATTCATCAGTTGTTTCGTCAAGCTAAATTTGATGATCCTAGTGGTGATTGTCTTAGTCCTGCTGGGGAGTACAATCTTCGACTTGGAATTATAAAGGAACTGCATCCTGACATGGTTGCTACTTATTCAGGCAGTGCTCAAGTTTTCGAGGGACATCCATTCATTGTTGAAGCTGGTGTTAGTATTGGAGGAAAAGATGTGAAACAGGGTCTGAATATTTTCCGGTTTGCTAATCGGATACCCCTTCTTTTTGAGCAAGGTGCTGATGTCGTTACTAGGACTGCACTTAAGAGGATCAATTGGAATAGTTATAAAATCAATCAGACTCAAGACAAAATTGGTGTGTTCGTGAGTATTGTTAGTACTAAGATTCCTTTCAAGGGAACTGGAAAGGAGTACATTGGAGTTGACATATCTGAAATAGCATCTGCTGTCAAGTCTGCCATTCAGCAGTGCTGCATTCAATTGAAAAGCAAAATAGTGAAGAAAATACAGGCACGCGAGCAACAGGAGAGGAAACGGAATTTGAGCAGATATATCCCGGATGCAACAAATGCTGTTTATAATGTTTTGAAAGAAATGGCTCAAGTGCATGCATCAAAGAAGAAACGTCATGACTGTGAGGATGCTGAAATACTCAGTAAAGTATCTGCTCGCTTGATTACAAATGAGACATTGAGGGATAAACTTGCACAACATGTTGAACAGGTCGACCACGAAATGGCCATGGAATATGCCACACAGAGTGGAGCAAGTGCAGAAGCCAAAGAAGAAATGTATATTCAATCACTGGATGCAACACATAACTGCACTGACTTCCAGAGCCCTTTGTTTGTTTTCAGACTGATTTGTTAA

Coding sequence (CDS)

ATGGAGGCAGGAGGTGGCAGTGAAAGTCCTGATGAGCCGAAGAAGGGCAAATCAAAAACACCCAGGAAGCCCAAAGAAAGCATCCTCAAGCAAAAATCACCAGCTGAGTTCTTTGCAGAAAACAAGAATATTGCTGGGTTTGATAATCCTGGGAAGTGTCTCTATACTACTGTGAGGGAGCTTGTGGAAAATTCTCTTGATTCTGCAGAGTCTATATCTGAGCTTCCCGTGGTAGAAATTACTATTGAAGAGATATTGAAAACCAAGTTTAATTCTATGATTGGTCTGGTTGATCGAGAACGTGTTGATGAGGAACTTTATGATGATTATGAGACTGCTAAGGCTCGTGAGAAAAGATTAGCGAAAGAAGCACGTGCTCAAGAAATACAGGCGAAAAATGCTGCCCTTGGGAAGAAAGGCAGAGAACCTGCAGCCTCTAAGGGTATGAAAGGTCGTGGTGAGGCTTCATTCTACCGGGTGACTTGCAAAGATAATGGAAAAGGAATGCCCCATGATGACATACCAAATATGTTTGGCCGAGTGCTCTCCGGAACAAAATATGGCTTGAAGCAAACCCGTGGAAAGTTTGGTCTGGGTGCAAAGATGGCATTAATTTGGTCTAAGATGAGTACTGGTCTTCCTATAGAGATATCATCATCAATGAAGGGTCAAAACTATACTTCATTCTGCCGCTTAGATATTGATATTCATCGGAACATTCCACACATTCATATCCAAGAAAAACGTGATAACAAGGATCTATGGCATGGGGCTGAAATTCAAGTTGTAATTGAAGGAAATTGGACAACTTACCGTTCGAAAATTTTGCATTATATGCGACAGATGGCAGTTATTACACCTTATGCTCAATTTCTTTTCAGATTTGTTTCAGACACACCTGATAAAAATATCACAATTAGATTTTCTCGAAGAACCGATGTTATGCCTCCAGTTCCTCTAGAGACTAAACACCATCCATCTGCTGTTGATTTACTGTTGATAAAGCGACTTATTGCAGAAACAGCTAAACAGAACCTTTTACAGTTTCTCCAGCATGAATTTGTAAACATAGGGAAGCCATTTGCTGAACGGTTAATTGGCGAAATGGGCCCAGAATTTAGCCCAAAAATGTCTGTTAAGTCTCTGACATCTCAGCAAATAGTCCGTATTCATCAGTTGTTTCGTCAAGCTAAATTTGATGATCCTAGTGGTGATTGTCTTAGTCCTGCTGGGGAGTACAATCTTCGACTTGGAATTATAAAGGAACTGCATCCTGACATGGTTGCTACTTATTCAGGCAGTGCTCAAGTTTTCGAGGGACATCCATTCATTGTTGAAGCTGGTGTTAGTATTGGAGGAAAAGATGTGAAACAGGGTCTGAATATTTTCCGGTTTGCTAATCGGATACCCCTTCTTTTTGAGCAAGGTGCTGATGTCGTTACTAGGACTGCACTTAAGAGGATCAATTGGAATAGTTATAAAATCAATCAGACTCAAGACAAAATTGGTGTGTTCGTGAGTATTGTTAGTACTAAGATTCCTTTCAAGGGAACTGGAAAGGAGTACATTGGAGTTGACATATCTGAAATAGCATCTGCTGTCAAGTCTGCCATTCAGCAGTGCTGCATTCAATTGAAAAGCAAAATAGTGAAGAAAATACAGGCACGCGAGCAACAGGAGAGGAAACGGAATTTGAGCAGATATATCCCGGATGCAACAAATGCTGTTTATAATGTTTTGAAAGAAATGGCTCAAGTGCATGCATCAAAGAAGAAACGTCATGACTGTGAGGATGCTGAAATACTCAGTAAAGTATCTGCTCGCTTGATTACAAATGAGACATTGAGGGATAAACTTGCACAACATGTTGAACAGGTCGACCACGAAATGGCCATGGAATATGCCACACAGAGTGGAGCAAGTGCAGAAGCCAAAGAAGAAATGTATATTCAATCACTGGATGCAACACATAACTGCACTGACTTCCAGAGCCCTTTGTTTGTTTTCAGACTGATTTGTTAA

Protein sequence

MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKPFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHNCTDFQSPLFVFRLIC*
Homology
BLAST of Chy5G091400 vs. ExPASy Swiss-Prot
Match: Q9C5V6 (DNA topoisomerase 6 subunit B OS=Arabidopsis thaliana OX=3702 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1087.4 bits (2811), Expect = 0.0e+00
Identity = 545/662 (82.33%), Postives = 601/662 (90.79%), Query Frame = 0

Query: 12  EPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAES 71
           E KKG SK  +   ES LKQKSPAEFFAENKNIAGFDNPGK LYTTVRELVEN+LDSAES
Sbjct: 8   ETKKGSSKNSKDSNESKLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAES 67

Query: 72  ISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQA 131
           ISELP VE+TIEEI+K+KFNSMIGL+DRERVD +LYDDYET KAR KRLAKEARA EIQA
Sbjct: 68  ISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKARGKRLAKEARASEIQA 127

Query: 132 KNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 191
           KN A GKK +EP  SK +K RGEAS+Y+VTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ
Sbjct: 128 KNLASGKKNKEPGVSKVLKARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 187

Query: 192 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDN 251
           TRGKFGLGAKMALIWSKMSTGLPIEISSSMK QNY +FCRLDIDIHRNIPHIH+ EK+ N
Sbjct: 188 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGN 247

Query: 252 KDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTPDKNITIRFSRR 311
           K+ WHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRF+S+TP+KN+TI+F+RR
Sbjct: 248 KEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFISETPEKNVTIKFTRR 307

Query: 312 TDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKPFAERLIGEMGP 371
           TDVMPP+P+ETKHHPS+VDLLLIKRLI +T+K+ LLQFLQ+EFVNI K  A RLIGEMGP
Sbjct: 308 TDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLAARLIGEMGP 367

Query: 372 EFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 431
           +F P M+VKS+TSQQ+VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY
Sbjct: 368 DFGPSMAVKSVTSQQMVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 427

Query: 432 SGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWN 491
           SGSAQVFEGHPFIVEAGVS+GG+DVKQG+NIFRFANRIPLLFEQGADVVTRTALKRINWN
Sbjct: 428 SGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWN 487

Query: 492 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQCCIQLKSKIVK 551
           SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIG DISEIA+AVKSAIQQCCIQLKSKIVK
Sbjct: 488 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVK 547

Query: 552 KIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLIT 611
           ++QAREQQERKR+LSRYIPDAT AVY VLK+M + H +K+KR+  ED  +L KVS ++IT
Sbjct: 548 RLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHKTKRKRYGEEDIVMLDKVSKQIIT 607

Query: 612 NETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDAT-HNCTDFQSPLFVFR 671
            ETL++KLA+HVEQVD+EMA+EYATQSG S E +E +Y+Q LD    N  D  SP FVFR
Sbjct: 608 KETLKEKLAEHVEQVDYEMALEYATQSGVSEEPRENIYLQHLDPNKSNFIDLHSPTFVFR 667

Query: 672 LI 673
           L+
Sbjct: 668 LM 669

BLAST of Chy5G091400 vs. ExPASy Swiss-Prot
Match: B8BDQ4 (DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. indica OX=39946 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1038.5 bits (2684), Expect = 3.3e-302
Identity = 521/677 (76.96%), Postives = 594/677 (87.74%), Query Frame = 0

Query: 3   AGGGSESPDEPKKGKSKTPRK-------PKESILKQKSPAEFFAENKNIAGFDNPGKCLY 62
           A   S +      GK K   K        + S+LKQKSPAEFFAENKNIAGFDNPGK LY
Sbjct: 20  AASSSAAAKGKAAGKGKAASKASALATAKESSLLKQKSPAEFFAENKNIAGFDNPGKSLY 79

Query: 63  TTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKA 122
           TT+RELVEN+LDSAESISELP +EI IEEI K+KFN+MIGLVDR+R+DEELYDD+E+AKA
Sbjct: 80  TTMRELVENALDSAESISELPDIEIIIEEITKSKFNTMIGLVDRQRIDEELYDDFESAKA 139

Query: 123 REKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIP 182
           REKRLAKEAR QE QAKNAALGKK +E  A++G KGRGEA+F+RVTCKDNG+GMPHDDIP
Sbjct: 140 REKRLAKEARFQETQAKNAALGKKVKEAPAARG-KGRGEAAFFRVTCKDNGRGMPHDDIP 199

Query: 183 NMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDID 242
           NM GRVLSGTKYGL+QTRGKFGLGAKMALIWSKMSTGLPIEI SSMKGQN+ SFCRLDID
Sbjct: 200 NMLGRVLSGTKYGLRQTRGKFGLGAKMALIWSKMSTGLPIEIKSSMKGQNFISFCRLDID 259

Query: 243 IHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRF 302
           IH+N+PH+H+ EKR+NKD WHGAE+QV+IEGNWTT+RSKILHYMRQMAVITPYAQFLFRF
Sbjct: 260 IHKNVPHVHLHEKRENKDRWHGAELQVIIEGNWTTHRSKILHYMRQMAVITPYAQFLFRF 319

Query: 303 VSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFV 362
           +SD+PDKN+TI+F+RRTDVMPP+PL+TKHHPSAVDLLLIKRLI+ET KQNLLQFLQHEFV
Sbjct: 320 LSDSPDKNLTIQFARRTDVMPPIPLQTKHHPSAVDLLLIKRLISETTKQNLLQFLQHEFV 379

Query: 363 NIGKPFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNL 422
           NI K  AERLIGEMGP+FS K +VKSLTSQQ+VRIHQLFRQAKFDDPSG+CLSPAGEYNL
Sbjct: 380 NISKSHAERLIGEMGPDFSAKTTVKSLTSQQLVRIHQLFRQAKFDDPSGNCLSPAGEYNL 439

Query: 423 RLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQ 482
           RLGIIKELHPD+VAT++ S QVFEGHPFIVEAG+SIGGKDVK GLNIFR+ANRIPLLFEQ
Sbjct: 440 RLGIIKELHPDLVATHASSPQVFEGHPFIVEAGISIGGKDVKHGLNIFRYANRIPLLFEQ 499

Query: 483 GADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVK 542
           GADV+TRTALKRINW+SYKINQ QDKIGVFVSIVSTKIPFKGTGKEYIG DI+EIASAV+
Sbjct: 500 GADVITRTALKRINWSSYKINQQQDKIGVFVSIVSTKIPFKGTGKEYIGDDITEIASAVQ 559

Query: 543 SAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHD 602
           SA++QCC+QLKSKIVKK+QARE+Q+RKRNL+RYIPD   A+   L E+A     K+ R+D
Sbjct: 560 SALKQCCLQLKSKIVKKLQARERQDRKRNLNRYIPDVARAIMETLGEIADESPPKRPRYD 619

Query: 603 CEDAEILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDA 662
            ED E+L KV++  +T  T RD L QHVEQVD+EMA+EYA QSG S E +E +Y+ SL+ 
Sbjct: 620 KEDEELLEKVNSEEVTEMTFRDCLTQHVEQVDYEMALEYAMQSGVSEEPREALYLNSLEG 679

Query: 663 THNCTDFQSPLFVFRLI 673
           ++   DFQSP+FVFR I
Sbjct: 680 SYKFIDFQSPVFVFRFI 695

BLAST of Chy5G091400 vs. ExPASy Swiss-Prot
Match: Q6H442 (DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=TOP6B PE=1 SV=1)

HSP 1 Score: 1035.8 bits (2677), Expect = 2.2e-301
Identity = 520/677 (76.81%), Postives = 593/677 (87.59%), Query Frame = 0

Query: 3   AGGGSESPDEPKKGKSKTPRK-------PKESILKQKSPAEFFAENKNIAGFDNPGKCLY 62
           A   S +      GK K   K        + S+LKQKSPAEFFAENKNIAGFDNPGK LY
Sbjct: 20  AASSSAAAKGKAAGKGKAASKASALATAKESSLLKQKSPAEFFAENKNIAGFDNPGKSLY 79

Query: 63  TTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKA 122
           TT+RELVEN+LDSAESISELP +EI IEEI K+KFN+MIGLVDR+R+DEELYDD+E+AKA
Sbjct: 80  TTMRELVENALDSAESISELPDIEIIIEEITKSKFNTMIGLVDRQRIDEELYDDFESAKA 139

Query: 123 REKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIP 182
           REKRLAKEAR QE QAKNAALGKK +E  A++G KGRGEA+F+RVTCKDNG+GMPHDDIP
Sbjct: 140 REKRLAKEARFQETQAKNAALGKKVKEAPAARG-KGRGEAAFFRVTCKDNGRGMPHDDIP 199

Query: 183 NMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDID 242
           NM GRVLSGTKYGL+QTRGKFGLGAKMALIWSKMSTGLPIEI SSMKGQN+ SFC LDID
Sbjct: 200 NMLGRVLSGTKYGLRQTRGKFGLGAKMALIWSKMSTGLPIEIKSSMKGQNFISFCLLDID 259

Query: 243 IHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRF 302
           IH+N+PH+H+ EKR+NKD WHGAE+QV+IEGNWTT+RSKILHYMRQMAVITPYAQFLFRF
Sbjct: 260 IHKNVPHVHLHEKRENKDRWHGAELQVIIEGNWTTHRSKILHYMRQMAVITPYAQFLFRF 319

Query: 303 VSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFV 362
           +SD+PDKN+TI+F+RRTDVMPP+PL+TKHHPSAVDLLLIKRLI+ET KQNLLQFLQHEFV
Sbjct: 320 LSDSPDKNLTIQFARRTDVMPPIPLQTKHHPSAVDLLLIKRLISETTKQNLLQFLQHEFV 379

Query: 363 NIGKPFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNL 422
           NI K  AERLIGEMGP+FS K +VKSLTSQQ+VRIHQLFRQAKFDDPSG+CLSPAGEYNL
Sbjct: 380 NISKSHAERLIGEMGPDFSAKTTVKSLTSQQLVRIHQLFRQAKFDDPSGNCLSPAGEYNL 439

Query: 423 RLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQ 482
           RLGIIKELHPD+VAT++ S QVFEGHPFIVEAG+SIGGKDVK GLNIFR+ANRIPLLFEQ
Sbjct: 440 RLGIIKELHPDLVATHASSPQVFEGHPFIVEAGISIGGKDVKHGLNIFRYANRIPLLFEQ 499

Query: 483 GADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVK 542
           GADV+TRTALKRINW+SYKINQ QDKIGVFVSIVSTKIPFKGTGKEYIG DI+EIASAV+
Sbjct: 500 GADVITRTALKRINWSSYKINQQQDKIGVFVSIVSTKIPFKGTGKEYIGDDITEIASAVQ 559

Query: 543 SAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHD 602
           SA++QCC+QLKSKIVKK+QARE+Q+RKRNL+RYIPD   A+   L E+A     K+ R+D
Sbjct: 560 SALKQCCLQLKSKIVKKLQARERQDRKRNLNRYIPDVARAIMETLGEIADESPPKRPRYD 619

Query: 603 CEDAEILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDA 662
            ED E+L KV++  +T  T RD L QHVEQVD+EMA+EYA QSG S E +E +Y+ SL+ 
Sbjct: 620 KEDEELLEKVNSEEVTEMTFRDCLTQHVEQVDYEMALEYAMQSGVSEEPREALYLNSLEG 679

Query: 663 THNCTDFQSPLFVFRLI 673
           ++   DFQSP+FVFR I
Sbjct: 680 SYKFIDFQSPVFVFRFI 695

BLAST of Chy5G091400 vs. ExPASy Swiss-Prot
Match: Q9YE64 (Type 2 DNA topoisomerase 6 subunit B OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) OX=272557 GN=top6B PE=3 SV=2)

HSP 1 Score: 308.1 bits (788), Expect = 2.4e-82
Identity = 188/584 (32.19%), Postives = 302/584 (51.71%), Query Frame = 0

Query: 30  KQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTK 89
           ++ S AEFFA+NK +AGF NP + LY T+RELVENSLD+ ++   LP + I+I +I    
Sbjct: 29  REMSVAEFFAKNKELAGFANPTRALYQTIRELVENSLDATDAKGILPWIHISIRQI---- 88

Query: 90  FNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGM 149
                                                                    +G 
Sbjct: 89  ---------------------------------------------------------EGS 148

Query: 150 KGRGEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKM 209
           +GRG    Y VT +DNG G+P   +   FG+VL  +KY ++QTRG +GLG K A+++ +M
Sbjct: 149 EGRGGRPRYTVTVEDNGIGVPVTSMAMAFGKVLFSSKYVIRQTRGMYGLGVKAAILYGQM 208

Query: 210 STGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWT 269
           + G P+E+ S+ KG  Y    +L ID+ RN P I  + + + +  WHG  + + +EG+W+
Sbjct: 209 TAGTPVEVYSATKGSQYVYMMKLVIDVQRNEPRILERGEWNKRGSWHGTRVTLSLEGDWS 268

Query: 270 TYRSKILHYMRQMAVITPYAQFLFRFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAV 329
             +SKIL Y+++ AVI PYA+ +     +TPD  I   F R T  +P  P E+K HP  V
Sbjct: 269 RAKSKILEYIKRTAVIAPYAEIIL----ETPDGEI-YYFPRSTRKLPKPPRESKPHPHGV 328

Query: 330 DLLLIKRLIAETAKQNLLQFLQHEFVNIGKPFAERLIGEMG--PEFSPKMSVKSLTSQQI 389
           D+  +K ++  T    L +FL  EF +IG   A+  + + G  P+  P+  +K    + +
Sbjct: 329 DIEQLKSMLRSTRASTLREFLVKEFQSIGDKTAQDFLEKYGLDPDLKPRELLKKGRDKLL 388

Query: 390 VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEA 449
            R+     +  F  P  D LSP G   + +G+ +  +P+ V   S S + + GHPFIVE 
Sbjct: 389 RRLADALMEYPFRAPKSDYLSPIGSDIIEIGLRRMFNPEWVGAVSRSPKAYRGHPFIVEV 448

Query: 450 GVSIGGK-DVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFV 509
           G++ GG  + +    + R+AN+IPLL+E+  D V+   +  INW  Y ++     + V V
Sbjct: 449 GIAYGGGIEARSEPLLLRYANKIPLLYEERED-VSYKVVSSINWRQYNVDFPAPLV-VLV 508

Query: 510 SIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLS 569
            I STK+P+KG GKE +  D+ EI + +++A+Q+   +L+  + +K +  E   R   L+
Sbjct: 509 HIASTKVPYKGVGKESVS-DVPEIEAEIRNAVQEVARRLRLYLSRKAREEEAIRRSVTLA 542

Query: 570 RYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLI 611
           +YIP+   ++   L+  ++    K +    +  E L K+ AR I
Sbjct: 569 KYIPEVAVSLAYFLRPPSKWQPPKPEEVK-KIQEALIKIVARHI 542

BLAST of Chy5G091400 vs. ExPASy Swiss-Prot
Match: C3NHT6 (Type 2 DNA topoisomerase 6 subunit B OS=Sulfolobus islandicus (strain Y.N.15.51 / Yellowstone #2) OX=419942 GN=top6B PE=3 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.6e-78
Identity = 198/603 (32.84%), Postives = 309/603 (51.24%), Query Frame = 0

Query: 33  SPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNS 92
           SPAEFF  N  +AGF NP + LY TVREL+ENSLD+ +    LP ++ITI+         
Sbjct: 11  SPAEFFKRNPELAGFPNPARALYQTVRELIENSLDATDVHGILPNIKITID--------- 70

Query: 93  MIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGR 152
              L+D  R                                                   
Sbjct: 71  ---LIDEAR--------------------------------------------------- 130

Query: 153 GEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTG 212
                Y+V   DNG G+P  ++PN FGRVL  +KY  +QTRG +GLG K A+++S+M   
Sbjct: 131 ---QIYKVNVVDNGIGIPPQEVPNAFGRVLYSSKYANRQTRGMYGLGVKAAVLYSQMHQD 190

Query: 213 LPIEISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYR 272
            PIEI +S          +L IDI++N P I  +   +N   +HG  + + I G+W   +
Sbjct: 191 KPIEIETSPANSKRIYTFKLKIDINKNEPIIVERGSVENTRGFHGTSVAISIPGDWPKAK 250

Query: 273 SKILHYMRQMAVITPYAQFLFRFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLL 332
           S+I  Y+++  +ITPYA+F+F+     P+ N+T  + R T+ +P  P E K HP  VD  
Sbjct: 251 SRIYEYIKRTYIITPYAEFIFK----DPEGNVTY-YPRLTNKIPKPPQEVKPHPYGVDRE 310

Query: 333 LIKRLIAETAKQ-NLLQFLQHEFVNIGKPFAERLIGEMGPEFSPKMSVKSLTSQQIVRIH 392
            IK LI    +   + +FL +EF +IG   A++++   G    P   VK+LT ++I R+ 
Sbjct: 311 EIKILINNLKRDYTIKEFLVNEFQSIGDTTADKILELAG--LKPNKKVKNLTEEEITRLV 370

Query: 393 QLFRQAK-FDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVS 452
           + F++ + F  PS D LS  GE  + LG+ K  +PD  A+ +   + ++GHPFIVEAGV+
Sbjct: 371 ETFKKYEDFRSPSADSLSVIGEDLIELGLKKIFNPDFAASITRKPKAYQGHPFIVEAGVA 430

Query: 453 IGGK-DVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIV 512
            GG   V +   + R+AN+IPL++++ +DV+ +  ++ ++W  Y I   Q ++ V V + 
Sbjct: 431 FGGSIPVGEEPIVLRYANKIPLIYDEKSDVIWK-VVEELDWKRYGIESDQYQMVVMVHLC 490

Query: 513 STKIPFKGTGKEYIGVDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNL---S 572
           STKIP+K  GKE I  ++ +I   +K+A+ +   +LK  + +K   R++QE K+ L    
Sbjct: 491 STKIPYKSAGKESI-AEVEDIEKEIKNALMEVARKLKQYLSEK---RKEQEAKKKLLAYL 530

Query: 573 RYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAQHVEQV 630
           +YIP+ + ++   L    +   SK +    E    L      LI  E  R      V +V
Sbjct: 551 KYIPEVSRSLATFLASGNKELVSKYQNEISEGLFKLISKKLDLINIEEYR-----KVYRV 530

BLAST of Chy5G091400 vs. ExPASy TrEMBL
Match: A0A5A7TIV7 (DNA topoisomerase 6 subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6B PE=3 SV=1)

HSP 1 Score: 1303.1 bits (3371), Expect = 0.0e+00
Identity = 665/673 (98.81%), Postives = 668/673 (99.26%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+FVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGK 
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIG DISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMAQVHASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHNCT 660
           ILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYATQSGASAE KEEMYIQSLDAT N T
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLIC 674
           DFQSPLFVFRLIC
Sbjct: 661 DFQSPLFVFRLIC 673

BLAST of Chy5G091400 vs. ExPASy TrEMBL
Match: A0A1S3BM58 (DNA topoisomerase 6 subunit B OS=Cucumis melo OX=3656 GN=LOC103491545 PE=3 SV=1)

HSP 1 Score: 1303.1 bits (3371), Expect = 0.0e+00
Identity = 665/673 (98.81%), Postives = 668/673 (99.26%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+FVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGK 
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIG DISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMAQVHASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHNCT 660
           ILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYATQSGASAE KEEMYIQSLDAT N T
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLIC 674
           DFQSPLFVFRLIC
Sbjct: 661 DFQSPLFVFRLIC 673

BLAST of Chy5G091400 vs. ExPASy TrEMBL
Match: A0A5D3DDA2 (DNA topoisomerase 6 subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6B PE=3 SV=1)

HSP 1 Score: 1274.6 bits (3297), Expect = 0.0e+00
Identity = 658/691 (95.22%), Postives = 661/691 (95.66%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDN----------- 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDN           
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNVSCIQLLSLSL 60

Query: 61  --------------PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120
                         PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG
Sbjct: 61  SLSPSLSLSVHFLRPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120

Query: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180
           LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA
Sbjct: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180

Query: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240
           SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI
Sbjct: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240

Query: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300
           EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI
Sbjct: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300

Query: 301 LHYMRQMAVITPYAQFLFRFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360
           LHYMRQMAVITPYAQFLF+FVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK
Sbjct: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360

Query: 361 RLIAETAKQNLLQFLQHEFVNIGKPFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420
           RLIAETAKQNLLQFLQHEFVNIGK FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR
Sbjct: 361 RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420

Query: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480
           QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD
Sbjct: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480

Query: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540
           VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF
Sbjct: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540

Query: 541 KGTGKEYIGVDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600
           KGTGKEYIG DISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA
Sbjct: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600

Query: 601 VYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYA 660
           +YNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYA
Sbjct: 601 IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA 660

Query: 661 TQSGASAEAKEEMYIQSLDATHNCTDFQSPL 667
           TQSGASAE KEEMYIQSLDAT N TDFQSPL
Sbjct: 661 TQSGASAEPKEEMYIQSLDATDNFTDFQSPL 691

BLAST of Chy5G091400 vs. ExPASy TrEMBL
Match: A0A6J1H1C2 (DNA topoisomerase 6 subunit B OS=Cucurbita moschata OX=3662 GN=LOC111459466 PE=3 SV=1)

HSP 1 Score: 1264.2 bits (3270), Expect = 0.0e+00
Identity = 644/672 (95.83%), Postives = 661/672 (98.36%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNA+LGKKGREPAASKGMKGRGEASFYRVTC+DNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCRDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300
           PH+H+QEKRDNKDLWHGAEIQVVIEGNWTTYRSKIL+YMRQMAVITPYAQFLF+FVSDTP
Sbjct: 241 PHVHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILYYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAETAKQ+LLQFLQHEFVN+GK 
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQ 540
           TRTALKRINW+SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIG DISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMA VHASKKKRHD E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHNCT 660
           ILSKVSAR+ITNETLRDKLAQHVEQVDHEMAMEYATQSGASAE+KEE+YIQSLDAT N T
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLI 673
           DF+SPLFVFRL+
Sbjct: 661 DFKSPLFVFRLV 672

BLAST of Chy5G091400 vs. ExPASy TrEMBL
Match: A0A6J1K0W3 (DNA topoisomerase 6 subunit B OS=Cucurbita maxima OX=3661 GN=LOC111490712 PE=3 SV=1)

HSP 1 Score: 1263.4 bits (3268), Expect = 0.0e+00
Identity = 644/672 (95.83%), Postives = 659/672 (98.07%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
            KEARAQEI AKNA+LGKKGREPA SKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 VKEARAQEILAKNASLGKKGREPAVSKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDI+RNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDINRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300
           PHIH+QEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+FVSDTP
Sbjct: 241 PHIHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360
           DKNI IRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAETAKQ+LLQFLQHEFVN+GK 
Sbjct: 301 DKNIAIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFFPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQ 540
           TRTALKRINW+SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIG DISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMA VHASKKKRHD E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHNCT 660
           ILSKVSAR+ITNETLRDKLAQHVEQVDHEMAMEYATQSGASAE+KEE+YIQSLDAT N T
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLI 673
           DF+SPLFVFRL+
Sbjct: 661 DFKSPLFVFRLV 672

BLAST of Chy5G091400 vs. NCBI nr
Match: XP_011653575.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucumis sativus] >KAE8649491.1 hypothetical protein Csa_018197 [Cucumis sativus])

HSP 1 Score: 1299 bits (3361), Expect = 0.0
Identity = 669/673 (99.41%), Postives = 670/673 (99.55%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQ QDKIGVFVSIVSTKIPFKGTGKEYIG DISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQAQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHNCT 660
           ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATH+ T
Sbjct: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHSFT 660

Query: 661 DFQSPLFVFRLIC 673
           DFQSPLFVFRLIC
Sbjct: 661 DFQSPLFVFRLIC 673

BLAST of Chy5G091400 vs. NCBI nr
Match: XP_008449755.1 (PREDICTED: DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo] >KAA0041365.1 DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1291 bits (3342), Expect = 0.0
Identity = 665/673 (98.81%), Postives = 668/673 (99.26%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300
           PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+FVSDTP
Sbjct: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGK 
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIG DISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA+YNVLKEMAQVHASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAIYNVLKEMAQVHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHNCT 660
           ILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYATQSGASAE KEEMYIQSLDAT N T
Sbjct: 601 ILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYATQSGASAEPKEEMYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLIC 673
           DFQSPLFVFRLIC
Sbjct: 661 DFQSPLFVFRLIC 673

BLAST of Chy5G091400 vs. NCBI nr
Match: XP_038902757.1 (DNA topoisomerase 6 subunit B isoform X1 [Benincasa hispida])

HSP 1 Score: 1275 bits (3299), Expect = 0.0
Identity = 657/672 (97.77%), Postives = 664/672 (98.81%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           M+AGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE
Sbjct: 1   MDAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNY SFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYISFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300
           PHIHIQEKRDNKD WHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+FVSDTP
Sbjct: 241 PHIHIQEKRDNKDQWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360
           DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGK 
Sbjct: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQ 540
           TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIG DISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQ HASKKKRHDCEDAE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQAHASKKKRHDCEDAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHNCT 660
           ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAE KEE+YIQSLDAT + T
Sbjct: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEPKEEIYIQSLDATDSFT 660

Query: 661 DFQSPLFVFRLI 672
           DF+SPLFVFRLI
Sbjct: 661 DFRSPLFVFRLI 672

BLAST of Chy5G091400 vs. NCBI nr
Match: TYK21651.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1263 bits (3267), Expect = 0.0
Identity = 658/691 (95.22%), Postives = 661/691 (95.66%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDN----------- 60
           MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDN           
Sbjct: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNVSCIQLLSLSL 60

Query: 61  --------------PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120
                         PGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG
Sbjct: 61  SLSPSLSLSVHFLRPGKCLYTTVRELVENSLDSAESISELPVVEITIEEILKTKFNSMIG 120

Query: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180
           LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA
Sbjct: 121 LVDRERVDEELYDDYETAKAREKRLAKEARAQEIQAKNAALGKKGREPAASKGMKGRGEA 180

Query: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240
           SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI
Sbjct: 181 SFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPI 240

Query: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300
           EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI
Sbjct: 241 EISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKI 300

Query: 301 LHYMRQMAVITPYAQFLFRFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360
           LHYMRQMAVITPYAQFLF+FVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK
Sbjct: 301 LHYMRQMAVITPYAQFLFKFVSDTPDKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIK 360

Query: 361 RLIAETAKQNLLQFLQHEFVNIGKPFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420
           RLIAETAKQNLLQFLQHEFVNIGK FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR
Sbjct: 361 RLIAETAKQNLLQFLQHEFVNIGKSFAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFR 420

Query: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480
           QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD
Sbjct: 421 QAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKD 480

Query: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540
           VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF
Sbjct: 481 VKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPF 540

Query: 541 KGTGKEYIGVDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600
           KGTGKEYIG DISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA
Sbjct: 541 KGTGKEYIGDDISEIASAVKSAIQQCCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNA 600

Query: 601 VYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYA 660
           +YNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLA+HVEQVDHEMAMEYA
Sbjct: 601 IYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLITNETLRDKLAEHVEQVDHEMAMEYA 660

Query: 661 TQSGASAEAKEEMYIQSLDATHNCTDFQSPL 666
           TQSGASAE KEEMYIQSLDAT N TDFQSPL
Sbjct: 661 TQSGASAEPKEEMYIQSLDATDNFTDFQSPL 691

BLAST of Chy5G091400 vs. NCBI nr
Match: XP_023532908.1 (DNA topoisomerase 6 subunit B isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1256 bits (3251), Expect = 0.0
Identity = 647/672 (96.28%), Postives = 661/672 (98.36%), Query Frame = 0

Query: 1   MEAGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRE 60
           ME GGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGK LYTTVRE
Sbjct: 1   MEIGGGSESPDEPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKSLYTTVRE 60

Query: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRL 120
           LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVD ELYDDYETAKAREKRL
Sbjct: 61  LVENSLDSAESISELPVVEITIEEILKTKFNSMIGLVDRERVDAELYDDYETAKAREKRL 120

Query: 121 AKEARAQEIQAKNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGR 180
           AKEARAQEIQAKNA+LGKKGREPAASKGMKGRGEASFYRVTCKDNG+GMPHDDIPNMFGR
Sbjct: 121 AKEARAQEIQAKNASLGKKGREPAASKGMKGRGEASFYRVTCKDNGRGMPHDDIPNMFGR 180

Query: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240
           VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI
Sbjct: 181 VLSGTKYGLKQTRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNI 240

Query: 241 PHIHIQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTP 300
           PHIH+QEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLF+FVSDTP
Sbjct: 241 PHIHVQEKRDNKDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFKFVSDTP 300

Query: 301 DKNITIRFSRRTDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKP 360
           DKNITIRFSRRTDVMPPVP ETKHHPSAVDLLLIKRLIAETAKQ+LLQFLQHEFVN+GK 
Sbjct: 301 DKNITIRFSRRTDVMPPVPQETKHHPSAVDLLLIKRLIAETAKQSLLQFLQHEFVNLGKS 360

Query: 361 FAERLIGEMGPEFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420
           +AERLIGEMGPEF PKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII
Sbjct: 361 YAERLIGEMGPEFCPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGII 420

Query: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480
           KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV
Sbjct: 421 KELHPDMVATYSGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVV 480

Query: 481 TRTALKRINWNSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQ 540
           TRTALKRINW+SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIG DISEIASAVKSAIQQ
Sbjct: 481 TRTALKRINWSSYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIASAVKSAIQQ 540

Query: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAE 600
           CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMA VHASKKKRHD E+AE
Sbjct: 541 CCIQLKSKIVKKIQAREQQERKRNLSRYIPDATNAVYNVLKEMAHVHASKKKRHDGENAE 600

Query: 601 ILSKVSARLITNETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDATHNCT 660
           ILSKVSAR+ITNETLRDKLAQHVEQVDHEMAMEYATQSGASAE+KEE+YIQSLDAT N T
Sbjct: 601 ILSKVSARVITNETLRDKLAQHVEQVDHEMAMEYATQSGASAESKEEIYIQSLDATDNFT 660

Query: 661 DFQSPLFVFRLI 672
           DF+SPLFVFRL+
Sbjct: 661 DFKSPLFVFRLV 672

BLAST of Chy5G091400 vs. TAIR 10
Match: AT3G20780.1 (topoisomerase 6 subunit B )

HSP 1 Score: 1087.4 bits (2811), Expect = 0.0e+00
Identity = 545/662 (82.33%), Postives = 601/662 (90.79%), Query Frame = 0

Query: 12  EPKKGKSKTPRKPKESILKQKSPAEFFAENKNIAGFDNPGKCLYTTVRELVENSLDSAES 71
           E KKG SK  +   ES LKQKSPAEFFAENKNIAGFDNPGK LYTTVRELVEN+LDSAES
Sbjct: 8   ETKKGSSKNSKDSNESKLKQKSPAEFFAENKNIAGFDNPGKSLYTTVRELVENALDSAES 67

Query: 72  ISELPVVEITIEEILKTKFNSMIGLVDRERVDEELYDDYETAKAREKRLAKEARAQEIQA 131
           ISELP VE+TIEEI+K+KFNSMIGL+DRERVD +LYDDYET KAR KRLAKEARA EIQA
Sbjct: 68  ISELPEVEVTIEEIVKSKFNSMIGLIDRERVDTQLYDDYETEKARGKRLAKEARASEIQA 127

Query: 132 KNAALGKKGREPAASKGMKGRGEASFYRVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 191
           KN A GKK +EP  SK +K RGEAS+Y+VTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ
Sbjct: 128 KNLASGKKNKEPGVSKVLKARGEASYYKVTCKDNGKGMPHDDIPNMFGRVLSGTKYGLKQ 187

Query: 192 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKGQNYTSFCRLDIDIHRNIPHIHIQEKRDN 251
           TRGKFGLGAKMALIWSKMSTGLPIEISSSMK QNY +FCRLDIDIHRNIPHIH+ EK+ N
Sbjct: 188 TRGKFGLGAKMALIWSKMSTGLPIEISSSMKSQNYVTFCRLDIDIHRNIPHIHLHEKKGN 247

Query: 252 KDLWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFVSDTPDKNITIRFSRR 311
           K+ WHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRF+S+TP+KN+TI+F+RR
Sbjct: 248 KEKWHGAEIQVVIEGNWTTYRSKILHYMRQMAVITPYAQFLFRFISETPEKNVTIKFTRR 307

Query: 312 TDVMPPVPLETKHHPSAVDLLLIKRLIAETAKQNLLQFLQHEFVNIGKPFAERLIGEMGP 371
           TDVMPP+P+ETKHHPS+VDLLLIKRLI +T+K+ LLQFLQ+EFVNI K  A RLIGEMGP
Sbjct: 308 TDVMPPIPIETKHHPSSVDLLLIKRLITDTSKKTLLQFLQNEFVNINKTLAARLIGEMGP 367

Query: 372 EFSPKMSVKSLTSQQIVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 431
           +F P M+VKS+TSQQ+VRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY
Sbjct: 368 DFGPSMAVKSVTSQQMVRIHQLFRQAKFDDPSGDCLSPAGEYNLRLGIIKELHPDMVATY 427

Query: 432 SGSAQVFEGHPFIVEAGVSIGGKDVKQGLNIFRFANRIPLLFEQGADVVTRTALKRINWN 491
           SGSAQVFEGHPFIVEAGVS+GG+DVKQG+NIFRFANRIPLLFEQGADVVTRTALKRINWN
Sbjct: 428 SGSAQVFEGHPFIVEAGVSLGGRDVKQGINIFRFANRIPLLFEQGADVVTRTALKRINWN 487

Query: 492 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGVDISEIASAVKSAIQQCCIQLKSKIVK 551
           SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIG DISEIA+AVKSAIQQCCIQLKSKIVK
Sbjct: 488 SYKINQTQDKIGVFVSIVSTKIPFKGTGKEYIGDDISEIATAVKSAIQQCCIQLKSKIVK 547

Query: 552 KIQAREQQERKRNLSRYIPDATNAVYNVLKEMAQVHASKKKRHDCEDAEILSKVSARLIT 611
           ++QAREQQERKR+LSRYIPDAT AVY VLK+M + H +K+KR+  ED  +L KVS ++IT
Sbjct: 548 RLQAREQQERKRSLSRYIPDATGAVYEVLKQMTEEHKTKRKRYGEEDIVMLDKVSKQIIT 607

Query: 612 NETLRDKLAQHVEQVDHEMAMEYATQSGASAEAKEEMYIQSLDAT-HNCTDFQSPLFVFR 671
            ETL++KLA+HVEQVD+EMA+EYATQSG S E +E +Y+Q LD    N  D  SP FVFR
Sbjct: 608 KETLKEKLAEHVEQVDYEMALEYATQSGVSEEPRENIYLQHLDPNKSNFIDLHSPTFVFR 667

Query: 672 LI 673
           L+
Sbjct: 668 LM 669

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C5V60.0e+0082.33DNA topoisomerase 6 subunit B OS=Arabidopsis thaliana OX=3702 GN=TOP6B PE=1 SV=1[more]
B8BDQ43.3e-30276.96DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. indica OX=39946 GN=TOP6B PE... [more]
Q6H4422.2e-30176.81DNA topoisomerase 6 subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=TOP6B ... [more]
Q9YE642.4e-8232.19Type 2 DNA topoisomerase 6 subunit B OS=Aeropyrum pernix (strain ATCC 700893 / D... [more]
C3NHT61.6e-7832.84Type 2 DNA topoisomerase 6 subunit B OS=Sulfolobus islandicus (strain Y.N.15.51 ... [more]
Match NameE-valueIdentityDescription
A0A5A7TIV70.0e+0098.81DNA topoisomerase 6 subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6B PE... [more]
A0A1S3BM580.0e+0098.81DNA topoisomerase 6 subunit B OS=Cucumis melo OX=3656 GN=LOC103491545 PE=3 SV=1[more]
A0A5D3DDA20.0e+0095.22DNA topoisomerase 6 subunit B OS=Cucumis melo var. makuwa OX=1194695 GN=TOP6B PE... [more]
A0A6J1H1C20.0e+0095.83DNA topoisomerase 6 subunit B OS=Cucurbita moschata OX=3662 GN=LOC111459466 PE=3... [more]
A0A6J1K0W30.0e+0095.83DNA topoisomerase 6 subunit B OS=Cucurbita maxima OX=3661 GN=LOC111490712 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_011653575.10.099.41DNA topoisomerase 6 subunit B isoform X1 [Cucumis sativus] >KAE8649491.1 hypothe... [more]
XP_008449755.10.098.81PREDICTED: DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo] >KAA0041365.1... [more]
XP_038902757.10.097.77DNA topoisomerase 6 subunit B isoform X1 [Benincasa hispida][more]
TYK21651.10.095.22DNA topoisomerase 6 subunit B isoform X1 [Cucumis melo var. makuwa][more]
XP_023532908.10.096.28DNA topoisomerase 6 subunit B isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G20780.10.0e+0082.33topoisomerase 6 subunit B [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 107..130
NoneNo IPR availablePFAMPF13589HATPase_c_3coord: 159..238
e-value: 7.9E-7
score: 29.1
NoneNo IPR availableGENE3D1.10.8.50coord: 323..400
e-value: 2.2E-17
score: 65.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..150
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..33
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..30
NoneNo IPR availablePANTHERPTHR1087130S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18coord: 1..655
IPR014721Ribosomal protein S5 domain 2-type fold, subgroupGENE3D3.30.230.10coord: 401..626
e-value: 1.5E-72
score: 245.4
IPR036890Histidine kinase/HSP90-like ATPase superfamilyGENE3D3.30.565.10coord: 23..322
e-value: 7.1E-70
score: 237.1
IPR036890Histidine kinase/HSP90-like ATPase superfamilySUPERFAMILY55874ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinasecoord: 155..294
IPR015320DNA topoisomerase VI, subunit B, transducerPFAMPF09239Topo-VIb_transcoord: 402..561
e-value: 4.5E-57
score: 192.3
IPR015320DNA topoisomerase VI, subunit B, transducerCDDcd00823TopoIIB_Transcoord: 411..561
e-value: 5.19265E-66
score: 212.146
IPR005734DNA topoisomerase VI, subunit BPANTHERPTHR10871:SF4DNA TOPOISOMERASE 6 SUBUNIT Bcoord: 1..655
IPR005734DNA topoisomerase VI, subunit BHAMAPMF_00322Top6Bcoord: 26..605
score: 28.52516
IPR010979Ribosomal protein S13-like, H2THSUPERFAMILY46946S13-like H2TH domaincoord: 324..396
IPR020568Ribosomal protein S5 domain 2-type foldSUPERFAMILY54211Ribosomal protein S5 domain 2-likecoord: 401..562

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy5G091400.1Chy5G091400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006265 DNA topological change
cellular_component GO:0009330 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) complex
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
molecular_function GO:0042803 protein homodimerization activity
molecular_function GO:0003676 nucleic acid binding