Chy4G089660 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G089660
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	RING-type domain-containing protein
Location	chrH04: 27181534 .. 27183360 (-)
RNA-Seq Expression	Chy4G089660
Synteny	Chy4G089660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGGTAGGAGTTGTTGCTGCTGATACTGGAAGTGGTGGATGGGAGTTTTTTGGTGTGGAAATTAACTCAAAATGCGAAAATCTTTCAAGGATTCCCTTAAGGCACTCGAGGCTGATATTCAGTTCGCCAATACCTTGTAATTTCTTCATATGCTGTTCAACTTTCTTATAATTTGATATCTACTCTGGTCTCTGGACTGTGTTTGAATTGCTATTGCAATGTCGGCGTTTCCATTGTCTGGAAATTATCCGCTATGTTTGTTAAATTTTCATGATTCGAACTGAATCGAGTTTCAATATTTTGTTTCTTGTGATATATGCTTTCTCCATGCTTAGATTAGAATGGTATTCGTTCGCTGCGCATGGAGTTTGAAGTTCAGGGAAGTTTCTATGAAAGTGGTTTGATAGGTTATGATTTCTGAGAGAGCCACGAGTTTTAGATTTTGCATCACATTATCTGTACATTCTTGACGACTTAGTAGATTAGATATTTAGTCATCTCGATCTTATTTCTGGAATTGTTCTCCGCCTGTTCATATATGTTATTTTCTGCTTAAGATTTATGAAACGGTTTATTTAAGATTTGTTATCAACTGCTGTGTTTCTTATTTTCTAGATGTTAAGTGTGTTGTAAGCTATCTCGGCTTCTCGGGATTGTATAACTACAGAATTTTACTGCAACAGATCATTTCTCTTGTTTTTAGTGTGCAATAGTTCCGGCAAGCCTTGTTTAATTCATAAGCATTGAAATATAATGCTTTAATACTGAACAGGGCTTCTGATTATCCCAAGGAATACGATGGAGCATGCCTGCAAATGAGATTATCCTATAGCCCGGCTGCGCAGTTTTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGCCTGCTCAGAATTCTAATATATAAGGTACTTCAGACTGCATGAAGTATGACATTTTGACTCCTACATTGCCCAAGTGTGGTGATTGAAGTGGATCAAACTGATCACTAAAGAAAAAAAATTTGATGAAGTAGCTTATACTACTTGCCTCTACACATAATCTTGTCAATCCCGTGAATGTGCTGAATTTGTTTTTAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGTAAGTGGCTAATAAGATAAAGACTGCCCTCTCGTCAGCTTGAGGTGTATTAGCATTTGGAGTCTTGCTAATTCTATTTTTTAGTTCTGCAAATACAGGGGTGATATTTCCTTCGCTAGTGCAACTTCAAAGAGGAATTACTGACATAGAAGAAAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAACAGATCGGTTGAACAAAGGAAAGATTTCTGAAATCGACTTGGAAAGAGAGGAAGAATGCGGTATTTGCATGGAGCTAAACAACAAGGTTGTCTTGCCTAATTGCAATCATTCAATGTGCATGAAGTGCTACAGGAGTTGGTATGCTCTCTGCTGTTTTCATGTGCTATGGTTACTAACTGAGGGCTACTTTGTTCTTAGCAAAAAAGTGAACTGGATTATGTGCAGCTGATTTTCTGCAACTTATTATCTCTTTTTCAGGCGCACTCGATCGCAGTCATGCCCCTTCTGCCGTGACAGTCTCAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATTGTCGACTTATCCTTCATCTCTGGGGAAAATCTGAAGAGGCTTTTCATGTTCATTAATAAGTTGCCTCTCATTGTTCCTGATCCAAAACTCATCTCTTATTATCTGAATCATTATTGA

mRNA sequence

ATGAGGGCTTCTGATTATCCCAAGGAATACGATGGAGCATGCCTGCAAATGAGATTATCCTATAGCCCGGCTGCGCAGTTTTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGCCTGCTCAGAATTCTAATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATATTTCCTTCGCTAGTGCAACTTCAAAGAGGAATTACTGACATAGAAGAAAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAACAGATCGGTTGAACAAAGGAAAGATTTCTGAAATCGACTTGGAAAGAGAGGAAGAATGCGGTATTTGCATGGAGCTAAACAACAAGGTTGTCTTGCCTAATTGCAATCATTCAATGTGCATGAAGTGCTACAGGAGTTGGCGCACTCGATCGCAGTCATGCCCCTTCTGCCGTGACAGTCTCAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATTGTCGACTTATCCTTCATCTCTGGGGAAAATCTGAAGAGGCTTTTCATGTTCATTAATAAGTTGCCTCTCATTGTTCCTGATCCAAAACTCATCTCTTATTATCTGAATCATTATTGA

Coding sequence (CDS)

ATGAGGGCTTCTGATTATCCCAAGGAATACGATGGAGCATGCCTGCAAATGAGATTATCCTATAGCCCGGCTGCGCAGTTTTTTCTCTTCTTCGTGCAATGGACTGATTGTCATCTTGCTGGTGCATTGGGCCTGCTCAGAATTCTAATATATAAGGCATATGAAGACGGGAAGACTACCATGTCTATTCAAGAAAGAAAGGCTAGTCTCAAAGAGTTCTATGGGGTGATATTTCCTTCGCTAGTGCAACTTCAAAGAGGAATTACTGACATAGAAGAAAGAAAACAGAGAGAAGTTTATGCTGCAAAATTCAAAAGAACAGATCGGTTGAACAAAGGAAAGATTTCTGAAATCGACTTGGAAAGAGAGGAAGAATGCGGTATTTGCATGGAGCTAAACAACAAGGTTGTCTTGCCTAATTGCAATCATTCAATGTGCATGAAGTGCTACAGGAGTTGGCGCACTCGATCGCAGTCATGCCCCTTCTGCCGTGACAGTCTCAAAAGAGTCAACTCTGGAGATCTTTGGATATGTACCAGTAGTAGTGAAATTGTCGACTTATCCTTCATCTCTGGGGAAAATCTGAAGAGGCTTTTCATGTTCATTAATAAGTTGCCTCTCATTGTTCCTGATCCAAAACTCATCTCTTATTATCTGAATCATTATTGA

Protein sequence

MRASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMSIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSSEIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY*

Homology

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BLAST of Chy4G089660 vs. ExPASy Swiss-Prot
Match: Q9M022 (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 6.7e-90
Identity = 153/215 (71.16%), Postives = 181/215 (84.19%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           AS+YP+EYDG  +QMRLSYSPAA  FLF +QWTDCH AGALGLLRILIYKAY DGKTTMS
Sbjct: 23  ASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           + ERK S++EFY V+FPSL+QL  GITD+EERKQ+E+   ++++ DR +KGK+SEIDLER
Sbjct: 83  LHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSEIDLER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGIC+E+ NKVVLP CNHSMC+ CYR+WR RSQSCPFCR SLKRVNSGDLWI T S+
Sbjct: 143 EEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWIYTCSA 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISY 218
           EI DL  I  ENLKRL ++I+KLPL+  DP L+ Y
Sbjct: 203 EIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

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BLAST of Chy4G089660 vs. ExPASy Swiss-Prot
Match: Q803C1 (E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 7.8e-06
Identity = 34/105 (32.38%), Postives = 51/105 (48.57%), Query Frame = 0


Query: 64  QERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK-FKRTDRLNKGKI----SEI 123
           ++ + +LKE   VI   L    +G  ++ + K +E+   K  K   +  K ++    +E+
Sbjct: 327 KQLEEALKEHRKVI-EELKHAWQGFKEVLQAKDKELEVTKEEKEKAKAQKEEVVTQMTEV 386

Query: 124 DLEREEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFC 164
            LE E +C IC EL  + V  NC HS C  C   WR R   CP C
Sbjct: 387 -LESELQCSICSELFIEAVTLNCAHSFCQHCISEWRNRKDKCPMC 429

[Close]

BLAST of Chy4G089660 vs. ExPASy Swiss-Prot
Match: Q13064 (Probable E3 ubiquitin-protein ligase makorin-3 OS=Homo sapiens OX=9606 GN=MKRN3 PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.3e-05
Identity = 26/71 (36.62%), Postives = 39/71 (54.93%), Query Frame = 0


Query: 123 EEECGICMEL-------NNK--VVLPNCNHSMCMKCYRSWRTRSQ-------SCPFCRDS 178
           ++ CGICME+       N++   +L NCNHS C++C R WR+  Q       SCP CR +
Sbjct: 308 DKVCGICMEVVYEKANPNDRRFGILSNCNHSFCIRCIRRWRSARQFENRIVKSCPQCRVT 367

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BLAST of Chy4G089660 vs. ExPASy Swiss-Prot
Match: Q13434 (Putative E3 ubiquitin-protein ligase makorin-4 OS=Homo sapiens OX=9606 GN=MKRN4P PE=5 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 2.3e-05
Identity = 24/76 (31.58%), Postives = 38/76 (50.00%), Query Frame = 0


Query: 118 IDLEREEECGICMEL---------NNKVVLPNCNHSMCMKCYRSWRTRSQ-------SCP 177
           +   +++ CGICME+         +   +L NCNH+ C+KC R WR+  +       SCP
Sbjct: 308 VQRSKDKVCGICMEVVYEKANPNEHRFGILSNCNHTFCLKCIRKWRSAKEFESRIVKSCP 367

[Close]

BLAST of Chy4G089660 vs. ExPASy Swiss-Prot
Match: Q9UHC7 (E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3)

HSP 1 Score: 50.1 bits (118), Expect = 3.9e-05
Identity = 25/76 (32.89%), Postives = 36/76 (47.37%), Query Frame = 0


Query: 118 IDLEREEECGICMEL---------NNKVVLPNCNHSMCMKCYRSWRTRSQ-------SCP 177
           +   ++  CGICME+             +L NCNH+ C+KC R WR+  Q       SCP
Sbjct: 273 VQRSKDMVCGICMEVVYEKANPSERRFGILSNCNHTYCLKCIRKWRSAKQFESKIIKSCP 332

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BLAST of Chy4G089660 vs. ExPASy TrEMBL
Match: A0A0A0LA78 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 PE=4 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.8e-122
Identity = 217/220 (98.64%), Postives = 219/220 (99.55%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS
Sbjct: 143 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 223
           EIVDLSFIS +NLKRLFMFINKLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. ExPASy TrEMBL
Match: A0A5D3DMZ7 (E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00850 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 9.9e-121
Identity = 214/220 (97.27%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK++RTD LNKGKISEID+ER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISEIDMER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELN KVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS
Sbjct: 143 EEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 223
           EIVDLSFIS ENLKRLFMFINKLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. ExPASy TrEMBL
Match: A0A1S3B5N9 (uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 9.9e-121
Identity = 214/220 (97.27%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK++RTD LNKGKISEID+ER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISEIDMER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELN KVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS
Sbjct: 143 EEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 223
           EIVDLSFIS ENLKRLFMFINKLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. ExPASy TrEMBL
Match: A0A6J1CVC0 (uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015127 PE=4 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 2.6e-113
Identity = 202/220 (91.82%), Postives = 212/220 (96.36%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAK+KR D+L++GKI+EIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELN  VVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI T SS
Sbjct: 143 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 223
           EIVDLSFIS ENLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. ExPASy TrEMBL
Match: A0A6J1F2V6 (LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441644 PE=4 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 6.4e-112
Identity = 199/220 (90.45%), Postives = 208/220 (94.55%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSLV LQRG  DIE+R+ RE YAAK+KR D+L+KGKISEIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLVLLQRGTNDIEDRQHREAYAAKYKRKDQLDKGKISEIDLER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELN+KVVLP C HSMCMKCYRSWR RSQSCPFCRDSL+RVNSGDLWICTSS+
Sbjct: 143 EEECGICMELNSKVVLPTCCHSMCMKCYRSWRARSQSCPFCRDSLRRVNSGDLWICTSST 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 223
           EIVDL FIS ENLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLPFISSENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. NCBI nr
Match: XP_011651863.1 (E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >KGN58763.1 hypothetical protein Csa_000946 [Cucumis sativus])

HSP 1 Score: 443 bits (1140), Expect = 1.26e-156
Identity = 217/220 (98.64%), Postives = 219/220 (99.55%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSLVQLQ+GITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQKGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS
Sbjct: 143 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 222
           EIVDLSFIS +NLKRLFMFINKLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRDNLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. NCBI nr
Match: XP_008442427.1 (PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa] >TYK24968.1 E3 ubiquitin-protein ligase RNF8-B [Cucumis melo var. makuwa])

HSP 1 Score: 437 bits (1125), Expect = 2.43e-154
Identity = 214/220 (97.27%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAK++RTD LNKGKISEID+ER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKYRRTDPLNKGKISEIDMER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELN KVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS
Sbjct: 143 EEECGICMELNTKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 222
           EIVDLSFIS ENLKRLFMFINKLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFINKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. NCBI nr
Match: XP_038904042.1 (E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida])

HSP 1 Score: 429 bits (1104), Expect = 3.86e-151
Identity = 211/220 (95.91%), Postives = 215/220 (97.73%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCH AGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHFAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKR DRL+KGKISEIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRKDRLDKGKISEIDLER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELN+KVVLP+CNHSMCMKCYRSWR RSQSCPFCRDSLKRVNSGDLWICTSSS
Sbjct: 143 EEECGICMELNSKVVLPSCNHSMCMKCYRSWRARSQSCPFCRDSLKRVNSGDLWICTSSS 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 222
           EIVDLSFIS ENLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. NCBI nr
Match: XP_022145744.1 (uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145745.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145746.1 uncharacterized protein LOC111015127 isoform X1 [Momordica charantia])

HSP 1 Score: 413 bits (1061), Expect = 1.38e-144
Identity = 202/220 (91.82%), Postives = 212/220 (96.36%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYP+EYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPREYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSL+QLQ GI DIE+RKQREVYAAK+KR D+L++GKI+EIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLLQLQGGINDIEDRKQREVYAAKYKRKDQLDRGKITEIDLER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELN  VVLP+CNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWI T SS
Sbjct: 143 EEECGICMELNCMVVLPSCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWIYTGSS 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 222
           EIVDLSFIS ENLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLSFISRENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. NCBI nr
Match: XP_023527445.1 (LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 409 bits (1052), Expect = 3.25e-143
Identity = 200/220 (90.91%), Postives = 208/220 (94.55%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           ASDYPKEY GACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS
Sbjct: 23  ASDYPKEYGGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           IQERKASLKEFYGVIFPSLV LQRG  DIE+R+ RE YAAK+KR D+L+KGKISEIDLER
Sbjct: 83  IQERKASLKEFYGVIFPSLVLLQRGTNDIEDRQHREAYAAKYKRKDQLDKGKISEIDLER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICMELN+KVVLP C HSMCMKCYRSWR RSQSCPFCRDSL+RVNSGDLWICTSSS
Sbjct: 143 EEECGICMELNSKVVLPTCCHSMCMKCYRSWRARSQSCPFCRDSLRRVNSGDLWICTSSS 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISYYLNHY 222
           EIVDL FIS ENLKRLFMFI+KLPLIVPDPKLISYYLNHY
Sbjct: 203 EIVDLPFISSENLKRLFMFIDKLPLIVPDPKLISYYLNHY 242

[Close]

BLAST of Chy4G089660 vs. TAIR 10
Match: AT5G01520.1 (RING/U-box superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 4.7e-91
Identity = 153/215 (71.16%), Postives = 181/215 (84.19%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           AS+YP+EYDG  +QMRLSYSPAA  FLF +QWTDCH AGALGLLRILIYKAY DGKTTMS
Sbjct: 23  ASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDGKTTMS 82

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           + ERK S++EFY V+FPSL+QL  GITD+EERKQ+E+   ++++ DR +KGK+SEIDLER
Sbjct: 83  LHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSEIDLER 142

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGIC+E+ NKVVLP CNHSMC+ CYR+WR RSQSCPFCR SLKRVNSGDLWI T S+
Sbjct: 143 EEECGICLEIRNKVVLPTCNHSMCINCYRNWRARSQSCPFCRGSLKRVNSGDLWIYTCSA 202

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPDPKLISY 218
           EI DL  I  ENLKRL ++I+KLPL+  DP L+ Y
Sbjct: 203 EIADLPAIYKENLKRLLIYIDKLPLVTSDPNLVPY 237

[Close]

BLAST of Chy4G089660 vs. TAIR 10
Match: AT3G47160.2 (RING/U-box superfamily protein )

HSP 1 Score: 314.7 bits (805), Expect = 6.0e-86
Identity = 144/210 (68.57%), Postives = 173/210 (82.38%), Query Frame = 0


Query: 2   RASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTM 61
           RA DYP+E DGA +QMRLSY+PAAQF LF VQWTDCHLAG LGLLR+LIY  Y DGKTTM
Sbjct: 35  RALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTTM 94

Query: 62  SIQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLE 121
           S+ ERK S+K+FY VIFPSL+QL+RGITD+++RKQ+EV   +++  D   K K+SEID+E
Sbjct: 95  SVYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDIE 154

Query: 122 REEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSS 181
           REEECGICME+NN VVLPNC HS+C+KCYR W  RS+SCPFCRDSLKRVNSGDLW+    
Sbjct: 155 REEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLMEK 214

Query: 182 SEIVDLSFISGENLKRLFMFINKLPLIVPD 212
           S+ V++  I  EN KRLF++I KLPL+VPD
Sbjct: 215 SDTVNMYTIERENKKRLFVYIEKLPLVVPD 244

[Close]

BLAST of Chy4G089660 vs. TAIR 10
Match: AT3G47160.1 (RING/U-box superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 2.3e-85
Identity = 143/209 (68.42%), Postives = 172/209 (82.30%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           A DYP+E DGA +QMRLSY+PAAQF LF VQWTDCHLAG LGLLR+LIY  Y DGKTTMS
Sbjct: 24  ALDYPREKDGARVQMRLSYTPAAQFLLFLVQWTDCHLAGTLGLLRVLIYMTYADGKTTMS 83

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           + ERK S+K+FY VIFPSL+QL+RGITD+++RKQ+EV   +++  D   K K+SEID+ER
Sbjct: 84  VYERKTSIKDFYAVIFPSLLQLERGITDLDDRKQKEVCKIRYRNKDETEKVKLSEIDIER 143

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICME+NN VVLPNC HS+C+KCYR W  RS+SCPFCRDSLKRVNSGDLW+    S
Sbjct: 144 EEECGICMEMNNMVVLPNCTHSLCIKCYRDWHGRSESCPFCRDSLKRVNSGDLWMLMEKS 203

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPD 212
           + V++  I  EN KRLF++I KLPL+VPD
Sbjct: 204 DTVNMYTIERENKKRLFVYIEKLPLVVPD 232

[Close]

BLAST of Chy4G089660 vs. TAIR 10
Match: AT5G58787.1 (RING/U-box superfamily protein )

HSP 1 Score: 300.4 bits (768), Expect = 1.2e-81
Identity = 141/209 (67.46%), Postives = 173/209 (82.78%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           A DYP+E DGA +QMRLSYSP AQFFLF VQWTDC LAG LGLLR+LIY  Y DGKTTMS
Sbjct: 24  ALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTTMS 83

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           + ERKAS++EF  VI PSL QLQRG+TDI++ KQ+EV   ++++ D   + ++SEI++ER
Sbjct: 84  VYERKASIREFQAVILPSLSQLQRGVTDIDDSKQKEVCKMRYRKKD---ESEMSEIEIER 143

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICME+N+KVVLPNC HS+C+KCYR WR RSQSCPFCRDSLKRV+SGDLW+    +
Sbjct: 144 EEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLDQN 203

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPD 212
           + V+L+ I+ EN KRLFM+I KLPL+VPD
Sbjct: 204 DTVNLTAIARENQKRLFMYIEKLPLVVPD 229

[Close]

BLAST of Chy4G089660 vs. TAIR 10
Match: AT5G58787.2 (RING/U-box superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 2.9e-72
Identity = 130/209 (62.20%), Postives = 161/209 (77.03%), Query Frame = 0


Query: 3   ASDYPKEYDGACLQMRLSYSPAAQFFLFFVQWTDCHLAGALGLLRILIYKAYEDGKTTMS 62
           A DYP+E DGA +QMRLSYSP AQFFLF VQWTDC LAG LGLLR+LIY  Y DGKTTMS
Sbjct: 24  ALDYPREKDGARVQMRLSYSPTAQFFLFLVQWTDCKLAGFLGLLRVLIYMTYADGKTTMS 83

Query: 63  IQERKASLKEFYGVIFPSLVQLQRGITDIEERKQREVYAAKFKRTDRLNKGKISEIDLER 122
           + ERKAS++EF                DI++ KQ+EV   ++++ D   + ++SEI++ER
Sbjct: 84  VYERKASIREF---------------QDIDDSKQKEVCKMRYRKKD---ESEMSEIEIER 143

Query: 123 EEECGICMELNNKVVLPNCNHSMCMKCYRSWRTRSQSCPFCRDSLKRVNSGDLWICTSSS 182
           EEECGICME+N+KVVLPNC HS+C+KCYR WR RSQSCPFCRDSLKRV+SGDLW+    +
Sbjct: 144 EEECGICMEMNSKVVLPNCTHSLCIKCYRDWRGRSQSCPFCRDSLKRVDSGDLWMFLDQN 203

Query: 183 EIVDLSFISGENLKRLFMFINKLPLIVPD 212
           + V+L+ I+ EN KRLFM+I KLPL+VPD
Sbjct: 204 DTVNLTAIARENQKRLFMYIEKLPLVVPD 214

The following BLAST results are available for this feature:

BLAST of Chy4G089660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M022	6.7e-90	71.16	E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 ...	[more]
Q803C1	7.8e-06	32.38	E3 ubiquitin-protein ligase rnf8 OS=Danio rerio OX=7955 GN=rnf8 PE=2 SV=1	[more]
Q13064	2.3e-05	36.62	Probable E3 ubiquitin-protein ligase makorin-3 OS=Homo sapiens OX=9606 GN=MKRN3 ...	[more]
Q13434	2.3e-05	31.58	Putative E3 ubiquitin-protein ligase makorin-4 OS=Homo sapiens OX=9606 GN=MKRN4P...	[more]
Q9UHC7	3.9e-05	32.89	E3 ubiquitin-protein ligase makorin-1 OS=Homo sapiens OX=9606 GN=MKRN1 PE=1 SV=3	[more]

BLAST of Chy4G089660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LA78	1.8e-122	98.64	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731740 P...	[more]
A0A5D3DMZ7	9.9e-121	97.27	E3 ubiquitin-protein ligase RNF8-B OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B5N9	9.9e-121	97.27	uncharacterized protein LOC103486301 OS=Cucumis melo OX=3656 GN=LOC103486301 PE=...	[more]
A0A6J1CVC0	2.6e-113	91.82	uncharacterized protein LOC111015127 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1F2V6	6.4e-112	90.45	LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 OS=Cuc...	[more]

BLAST of Chy4G089660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011651863.1	1.26e-156	98.64	E3 ubiquitin-protein ligase AIRP2 [Cucumis sativus] >XP_031738614.1 E3 ubiquitin...	[more]
XP_008442427.1	2.43e-154	97.27	PREDICTED: uncharacterized protein LOC103486301 [Cucumis melo] >KAA0044165.1 E3 ...	[more]
XP_038904042.1	3.86e-151	95.91	E3 ubiquitin-protein ligase AIRP2 isoform X1 [Benincasa hispida]	[more]
XP_022145744.1	1.38e-144	91.82	uncharacterized protein LOC111015127 isoform X1 [Momordica charantia] >XP_022145...	[more]
XP_023527445.1	3.25e-143	90.91	LON peptidase N-terminal domain and RING finger protein 1-like isoform X2 [Cucur...	[more]

BLAST of Chy4G089660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G01520.1	4.7e-91	71.16	RING/U-box superfamily protein	[more]
AT3G47160.2	6.0e-86	68.57	RING/U-box superfamily protein	[more]
AT3G47160.1	2.3e-85	68.42	RING/U-box superfamily protein	[more]
AT5G58787.1	1.2e-81	67.46	RING/U-box superfamily protein	[more]
AT5G58787.2	2.9e-72	62.20	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 126..163 e-value: 2.0E-6 score: 37.3
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 126..164 score: 12.179465
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 123..169 e-value: 1.4E-9 score: 37.7
None	No IPR available	PANTHER	PTHR15315	RING FINGER PROTEIN 41, 151	coord: 3..219
None	No IPR available	PANTHER	PTHR15315:SF31	E3 UBIQUITIN-PROTEIN LIGASE AIRP2	coord: 3..219
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 108..169
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 100..172 e-value: 2.7E-15 score: 57.5
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 141..150

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G089660.1	Chy4G089660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009789	positive regulation of abscisic acid-activated signaling pathway
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0009651	response to salt stress
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0061630	ubiquitin protein ligase activity