Chy4G084170 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G084170
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Cytoplasmic tRNA 2-thiolation protein 2
Location	chrH04: 23417055 .. 23420356 (+)
RNA-Seq Expression	Chy4G084170
Synteny	Chy4G084170

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATGTAATTCCTCAGCCTGCCAGTCCAACTGCTACAGAAATCAACATGATGAACATACAATCGAACGAGATTCACCTCCTTCAAGCAGGCCCGATACAGCAGCTGCCTTGGAGGCTCGTCGAGTTATCACGAACGACGACAATAGTAATACGAATCTCTGCTTTAAATGCAAGTCCAAGGAACGTATGTCTACCTCCGGAGCAGGAGACGATGGACGTTTTTGCAGTGATTGCTTTCGGAGCAACTTGTTTGCTAAGTTCCGGCAGGCTGTAACCTCCAATGCCATGATCTCTCCTTCTGATAAAGTGCTTGTTGCGTTCTCTGGAGGCCCTTGTTCCAGGTATTTTCTCTTATATATATATATATATATATATATATCTATATGTATGTATGTATGGATGGATGGATATTTGGACGTTGCTAGTGGGCAGAATAGATTTTACGGCTGAATTTTGTGTTAGAATCCATAGTGCCAATGAGTTTGTAATGTTTTAATGTTATTCGTGATCGACTTAACCTCCTTTCCCAATCACTTCCAGGGTGGCTTTGCAGTTTGTTCATGAAATGCAAGTAAAAGCACACAATAATTTTGAAGCAAGTAGGGATAGATCATTGCAAGTTTTTGGTGTTGGAGTTGCTTATATCAATGAAACTGCTGCTTGTTCTATTCCTTCGGACGAGATAGACAAAACAATTCAAACCATCCAATCGGTTGTGTCAAGTCTATGTCAACCAAGAAAAAATTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGAGTGATGGAAGGGAGAAGTTGAAGAAACTGTTAGATGCAGTCAATGACGTCACAGGAAAAGAAGACCTTATCTCATATTTGCGGATGTTATCCTTGCAAAAAGTTTGCCCCTCAATTGTTCCTTCATTTAGCTTGCAGTGAAAGAGGGGGAAATTCTCAGCCTGTTGTTTGTTGTGCAGATTGCAGCTGAAAATGGGTACAACAGACTTCTAGTTGGAACATGTACTTCAAGGATTGCATGCCATGTGATTTCGTCTACCGTTAAGGTTTTCCTTCGTTTGCCCCCCTCCCCCCCCCTCTAATATTCATTTGTATTTTAAGAGTCACTAGAAATTTTAGAGTCATGATAATGAATGACCAGAAGGAAGGTTATAAGGTTGATGCATTGACTGTTAAGGGTCCATGGATACGAGAACTCTGTTATTTGAGATAGAACAGAACAATTGGTCTATGCTTCAATCTTAGATCTTGAGGATATTATGTAAATAAGATCCAGTAATATGGCAAGCAAACACGTTGCTTATACAAAAATCTGTTTCCATGCTAAAAAATCCCGTAAAAATTGAACCCTCAAACTCACATAGTTGTAGAAATTGTAACAAATGCTTAAGTTTGAGGGTTTGATTTTTAGCATTCGAGGGTCAAATTTTTGTAACTATTGTAAGTTTGTGGGGGCCTTAATATTTACCATTGAAAACTTGAGGATATAATTCCAGTTACCACCATGGTTCAAAGGTGATTCTTACAATTTGTCCACAATAATATCATTTTTATCGGATTATTAACTAAGAGCTTGCCATGCACACTTTTATTTATCGTTCAGACAAAGTCAAGAGTGGAACTACTCTCTGCGGGGAAAACTGGGATGCTTTAAGGGGAGAAAGGGGGGGATCATTATCTTTCAGAAGACACCTCTATTAAGACGTAAAAAATAGCTAGGGTAGCTATGGAATCTTATGATGAATTATCTTGTTGTTTGAGATTTCACTACTACTTATTACAAGCTGCTTATCTTTTTCTACTTGAAAATCTTTCCGTTTGAGTTAGGTCCATGCACTAATTTTGACTACACTATTAATTCAAGCAGGGCCAAGGGTATTCCTTGCCGGCAGATGTACAGTATATTGATGCAAGGTGGGAGGTTCCAGTTGTACTTCCTCTACTTGACTGTCTTGCGCAAGAGCTCAACATGCTTTGCTCCGTTGATGGGTTTGTTAATTTTGTCCTCCATTCTTGGTTAACTTTGTCCTGTTTAGTACTTTACTGTCGTTAAATTTACTTGCTGTTGCATCTTTAATATGTTCACTTCGACTGAATCTAATTGATGATGGGAGCTGTTTCTTGGCAGGGCAGTCTAAAGACAGTAGAACTGACCAAAAAGTCCAGTTCTGGCATCAATGACCTGGTCTCCTCATTTGTGGCTCTTTTGCAGGTACAAGTAGGAAGTTCATTTAGAAAAATGTGCAGTTTTATTCATTTTGGCTGGAGAGCAACTTCTTGAAGCATGTTTTGGTTGAGTATGGAAACAATCACGCAAAGTATGGAAACAATTTTACCTTCTATTTTGCGTGATTGTTTCCGAGTAAGAAAGAGATTGTTCATTCAAGTTGTAGGCTACTTGTCCTACCCAGGAATGTTTTTCAGAACTTGACCGAAGATTTTTTTTCTTTTTCTACTTGCCTTTATAGGTAGAAAATCCTTCCCGTGAGTCTACGATTGTGAGAACTGCTGGCAAGCTTACTCCATTCAATTTTAGCAGAATACCAGATTTGAACGAAAGTAATAATGTTCCTTTGGCCACGAAAAGGCGTCAACAGAGAAATAACTTTAAGCATGTTAACTCTTTATCTTCGGAGTCATTTTGTGCAGTCTGTAATGGGCCACTTAACACGTCAGATGTGCAAACTCGCCATGCAAGTTCAAGTATTTCGGGTGCTTGTTGTTCAAGTTGTCAATTTCAGATTCTTCCCAAGGATTCTTCGTCTCAAGAACTGTTTCTTTCACTCTTGCCAACCTCTATGGTTGCTCGAGCCACACATGGCTGCCTTGGCAATTCCAATTCGCTAAGGTGAGAGTGAGATTAAGTCTTGTATTACTGTCGGTACTACATAGTTCAATTTTATGGTAACTGAATATTGTAATCTAAATCTCTGTTGTATATCATCTTTCTAAAAGAGGTTTCCCTTCGATTCTGGACCGAAGCACATCAAAGAAATCAAACGTAAAATATAAGGAAAATTAAACGACTTCAGCCTGTGGTTTACATGATTCAAAAAATGTCTATTTGAGTCACGTTAGGTGGACTCGATGAAGTTATCGAAATATTGTTGTTTTATGTTGTAAATAGTAATGTGCTTAAAGTGTATACTAGCATGAAGTCTGCCTCAGATATTACTATGTTGAACTTATTAAGTAATGCTTTTTGAAAATATCCCCGTTAATTAGTTATGTTATTAATTGCAGGAAGAAAATACAGGAGTTCTTACTCTCAGATGATGAAGCTGAATCATAA

mRNA sequence

ATGGCATGTAATTCCTCAGCCTGCCAGTCCAACTGCTACAGAAATCAACATGATGAACATACAATCGAACGAGATTCACCTCCTTCAAGCAGGCCCGATACAGCAGCTGCCTTGGAGGCTCGTCGAGTTATCACGAACGACGACAATAGTAATACGAATCTCTGCTTTAAATGCAAGTCCAAGGAACGTATGTCTACCTCCGGAGCAGGAGACGATGGACGTTTTTGCAGTGATTGCTTTCGGAGCAACTTGTTTGCTAAGTTCCGGCAGGCTGTAACCTCCAATGCCATGATCTCTCCTTCTGATAAAGTGCTTGTTGCGTTCTCTGGAGGCCCTTGTTCCAGGGTGGCTTTGCAGTTTGTTCATGAAATGCAAGTAAAAGCACACAATAATTTTGAAGCAAGTAGGGATAGATCATTGCAAGTTTTTGGTGTTGGAGTTGCTTATATCAATGAAACTGCTGCTTGTTCTATTCCTTCGGACGAGATAGACAAAACAATTCAAACCATCCAATCGGTTGTGTCAAGTCTATGTCAACCAAGAAAAAATTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGAGTGATGGAAGGGAGAAGTTGAAGAAACTGTTAGATGCAGTCAATGACGTCACAGGAAAAGAAGACCTTATCTCATATTTGCGGATGTTATCCTTGCAAAAAATTGCAGCTGAAAATGGGTACAACAGACTTCTAGTTGGAACATGTACTTCAAGGATTGCATGCCATGTGATTTCGTCTACCGTTAAGGGCCAAGGGTATTCCTTGCCGGCAGATGTACAGTATATTGATGCAAGGTGGGAGGTTCCAGTTGTACTTCCTCTACTTGACTGTCTTGCGCAAGAGCTCAACATGCTTTGCTCCGTTGATGGTCTAAAGACAGTAGAACTGACCAAAAAGTCCAGTTCTGGCATCAATGACCTGGTCTCCTCATTTGTGGCTCTTTTGCAGGTAGAAAATCCTTCCCGTGAGTCTACGATTGTGAGAACTGCTGGCAAGCTTACTCCATTCAATTTTAGCAGAATACCAGATTTGAACGAAAGTAATAATGTTCCTTTGGCCACGAAAAGGCGTCAACAGAGAAATAACTTTAAGCATGTTAACTCTTTATCTTCGGAGTCATTTTGTGCAGTCTGTAATGGGCCACTTAACACGTCAGATGTGCAAACTCGCCATGCAAGTTCAAGTATTTCGGGTGCTTGTTGTTCAAGTTGTCAATTTCAGATTCTTCCCAAGGATTCTTCGTCTCAAGAACTGTTTCTTTCACTCTTGCCAACCTCTATGGTTGCTCGAGCCACACATGGCTGCCTTGGCAATTCCAATTCGCTAAGGAAGAAAATACAGGAGTTCTTACTCTCAGATGATGAAGCTGAATCATAA

Coding sequence (CDS)

ATGGCATGTAATTCCTCAGCCTGCCAGTCCAACTGCTACAGAAATCAACATGATGAACATACAATCGAACGAGATTCACCTCCTTCAAGCAGGCCCGATACAGCAGCTGCCTTGGAGGCTCGTCGAGTTATCACGAACGACGACAATAGTAATACGAATCTCTGCTTTAAATGCAAGTCCAAGGAACGTATGTCTACCTCCGGAGCAGGAGACGATGGACGTTTTTGCAGTGATTGCTTTCGGAGCAACTTGTTTGCTAAGTTCCGGCAGGCTGTAACCTCCAATGCCATGATCTCTCCTTCTGATAAAGTGCTTGTTGCGTTCTCTGGAGGCCCTTGTTCCAGGGTGGCTTTGCAGTTTGTTCATGAAATGCAAGTAAAAGCACACAATAATTTTGAAGCAAGTAGGGATAGATCATTGCAAGTTTTTGGTGTTGGAGTTGCTTATATCAATGAAACTGCTGCTTGTTCTATTCCTTCGGACGAGATAGACAAAACAATTCAAACCATCCAATCGGTTGTGTCAAGTCTATGTCAACCAAGAAAAAATTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGAGTGATGGAAGGGAGAAGTTGAAGAAACTGTTAGATGCAGTCAATGACGTCACAGGAAAAGAAGACCTTATCTCATATTTGCGGATGTTATCCTTGCAAAAAATTGCAGCTGAAAATGGGTACAACAGACTTCTAGTTGGAACATGTACTTCAAGGATTGCATGCCATGTGATTTCGTCTACCGTTAAGGGCCAAGGGTATTCCTTGCCGGCAGATGTACAGTATATTGATGCAAGGTGGGAGGTTCCAGTTGTACTTCCTCTACTTGACTGTCTTGCGCAAGAGCTCAACATGCTTTGCTCCGTTGATGGTCTAAAGACAGTAGAACTGACCAAAAAGTCCAGTTCTGGCATCAATGACCTGGTCTCCTCATTTGTGGCTCTTTTGCAGGTAGAAAATCCTTCCCGTGAGTCTACGATTGTGAGAACTGCTGGCAAGCTTACTCCATTCAATTTTAGCAGAATACCAGATTTGAACGAAAGTAATAATGTTCCTTTGGCCACGAAAAGGCGTCAACAGAGAAATAACTTTAAGCATGTTAACTCTTTATCTTCGGAGTCATTTTGTGCAGTCTGTAATGGGCCACTTAACACGTCAGATGTGCAAACTCGCCATGCAAGTTCAAGTATTTCGGGTGCTTGTTGTTCAAGTTGTCAATTTCAGATTCTTCCCAAGGATTCTTCGTCTCAAGAACTGTTTCTTTCACTCTTGCCAACCTCTATGGTTGCTCGAGCCACACATGGCTGCCTTGGCAATTCCAATTCGCTAAGGAAGAAAATACAGGAGTTCTTACTCTCAGATGATGAAGCTGAATCATAA

Protein sequence

MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKSKERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFVHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQPRKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVDGLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSISGACCSSCQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES*

Homology

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BLAST of Chy4G084170 vs. ExPASy Swiss-Prot
Match: O65628 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 PE=2 SV=3)

HSP 1 Score: 495.0 bits (1273), Expect = 9.6e-139
Identity = 261/473 (55.18%), Postives = 340/473 (71.88%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSS C+S CY  + D  +   D   S                    ++ ++C KCK 
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDDAVSG-----------------GGNHESVCVKCKC 60

Query: 61  KERMSTSGAG-DDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQ 120
              M+    G DDGRFC+DCFR+N+F KFR AVTS+AMI+PSD VLVAFSGG  SRV+LQ
Sbjct: 61  NAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQ 120

Query: 121 FVHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQ 180
           FVHE+Q+KA  N+EASRDRSL VFGVGVA+++ETAA    S E+   I+ ++  VS L  
Sbjct: 121 FVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSP 180

Query: 181 PRKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYN 240
           P K+LH+V +ES++ S+  D R++L KLLD+V D TGKEDL+ +L+MLSLQK+AAENGYN
Sbjct: 181 PAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYN 240

Query: 241 RLLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSV 300
           RL++G+CTSRIA HV+++TVKG+GYSL AD+Q++DARW+VP+VLPL DC+  E+  LC +
Sbjct: 241 RLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLL 300

Query: 301 DGLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESN 360
           DGLKTVEL  +S  GINDLVSSFVALLQ ENPSRE TIVRTA KLTPF F++IP+ ++S 
Sbjct: 301 DGLKTVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDS- 360

Query: 361 NVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSD------VQTRHASSSISGACCS 420
           NVP+AT+RR +R N K+  S+++E+FC +CNGPLN SD       +    S  +  ACCS
Sbjct: 361 NVPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCS 420

Query: 421 SCQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDE 467
           SC+FQILP+D SS E F S LP  M+++  H  + +   LR+KI++ LL DDE
Sbjct: 421 SCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455

[Close]

BLAST of Chy4G084170 vs. ExPASy Swiss-Prot
Match: Q2QMW0 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CTU2 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.7e-103
Identity = 214/474 (45.15%), Postives = 300/474 (63.29%), Query Frame = 0


Query: 2   ACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKSK 61
           +C  + C  +C             S  ++  + AAA  A +V      S +  C KC   
Sbjct: 8   SCGGAGCGPHC-----------SSSASAAAVEDAAAAAAEKV---GRLSLSRECGKC-GG 67

Query: 62  ERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 121
              + + AG  G  C +CFR+NL  KF+ AVTSNAM+ P+D VL+AFSGGP SRVALQF+
Sbjct: 68  GAAAVAVAGGLG-LCGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFI 127

Query: 122 HEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQPR 181
           HEM+ KA  +++ S  ++L VFGVGVA+++E+  CS P DEI+  I+ I+S+VSSL    
Sbjct: 128 HEMRCKAIESWDVSNSQALPVFGVGVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGV 187

Query: 182 KNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNRL 241
           K +HI ++E V+S+   DG  +L++ +D + D TG+ED +  LRMLSLQKIA ENGY ++
Sbjct: 188 KAMHIARLEDVFSTESEDGERRLREAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKI 247

Query: 242 LVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVDG 301
           ++G+C S IACHV+S+TVKGQGYSLPADVQY+D RWE+PVVLPL DCLAQEL +LC +D 
Sbjct: 248 MLGSCASAIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDS 307

Query: 302 LKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNV 361
           LKT +   + S+GIN LV+SF+  L+ ENPSRE TIVRTA KL PF+F++       + +
Sbjct: 308 LKTQQHLDRPSNGINSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFL 367

Query: 362 PLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQ----TRHASSS----ISGACCS 421
           P  ++ R +   F    S  SE  C +C  P + S++Q    T+H +       +  CC 
Sbjct: 368 P--SRLRPKFQKFDSDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQ 427

Query: 422 SCQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNS-LRKKIQEFLLSDDE 467
           SC FQILP   +  E F SLLP     +     + +S+S LR +I+E+LL +++
Sbjct: 428 SCYFQILPAGENLNEHFFSLLPKLWTGKM--DTISDSHSLLRDQIEEYLLEEND 461

[Close]

BLAST of Chy4G084170 vs. ExPASy Swiss-Prot
Match: A2ZM73 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=CTU2 PE=3 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 3.8e-103
Identity = 214/474 (45.15%), Postives = 300/474 (63.29%), Query Frame = 0


Query: 2   ACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKSK 61
           +C  + C  +C             S  ++  + AAA  A +V      S +  C KC   
Sbjct: 8   SCGGAGCGPHC-----------SSSASAAAVEDAAAAAAEKV---GRLSLSRECGKC-GG 67

Query: 62  ERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQFV 121
              + + AG  G  C +CFR+NL  KF+ AVTSNAM+ P+D VL+AFSGGP SRVALQF+
Sbjct: 68  GAAAVAVAGGLG-LCGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGPASRVALQFI 127

Query: 122 HEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQPR 181
           HEM+ KA  +++ S  ++L VFGVGVA+++E+  CS P DEI+  I+ I+S+VSSL    
Sbjct: 128 HEMRCKAIESWDVSNSQALPVFGVGVAFVDESVLCSKPRDEIEMAIEDIRSIVSSLSTGV 187

Query: 182 KNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNRL 241
           K +HIV++E V+S+   DG  +L++ +D + D TG+ED +  LRMLSLQKIA ENGY ++
Sbjct: 188 KAMHIVRLEDVFSTESEDGERRLREAVDMIGDDTGREDFLRCLRMLSLQKIAMENGYAKI 247

Query: 242 LVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVDG 301
           ++G+C S IACHV+S+TVKGQGYSLPADVQY+D RWE+PVVLPL DCLAQEL +LC +D 
Sbjct: 248 MLGSCASAIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDCLAQELTLLCELDS 307

Query: 302 LKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNNV 361
           LKT +   + S+GIN LV+SF+  L+ ENPSRE TIVRTA KL PF+F++       + +
Sbjct: 308 LKTQQHLDRPSNGINSLVASFIKRLREENPSREHTIVRTAQKLKPFSFNKFSADGYHDFL 367

Query: 362 PLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQ----TRHASSS----ISGACCS 421
           P  ++ R +        S  SE  C +C  P + S++Q    T+H +       +  CC 
Sbjct: 368 P--SRLRPKFQKVDSDESTFSEILCLMCGSPFSESELQNLESTKHKAQKKIDLYTAHCCQ 427

Query: 422 SCQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNS-LRKKIQEFLLSDDE 467
           SC FQILP   +  E F SLLP     +     + +S+S LR +I+E+LL +++
Sbjct: 428 SCYFQILPAGENLNEHFFSLLPKLWTGKM--DTISDSHSLLRDQIEEYLLEEND 461

[Close]

BLAST of Chy4G084170 vs. ExPASy Swiss-Prot
Match: Q08B12 (Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE=2 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.4e-25
Identity = 135/514 (26.26%), Postives = 212/514 (41.25%), Query Frame = 0


Query: 54  LCFKCKSKERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPC 113
           +C KCK         AGD   FC  CF+     KFR  +  N +I P +KVL+A+SGGP 
Sbjct: 24  ICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAYSGGPS 83

Query: 114 SRVALQFVHEMQVKAHNNFEASRDRSLQV-FGVGVAYINETAACSIPSDEIDKTIQTIQS 173
           S   ++ V E           SRD   ++ F  G+ +I+E  AC +  +E     Q +  
Sbjct: 84  SSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEERQ---QILSE 143

Query: 174 VVSSLCQPRKNLHIVQIESVYS---SNLSDG----------------------------- 233
           + S L Q +   HIV +E V+S   S L  G                             
Sbjct: 144 ICSVLQQTKIPFHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQEREQGDAGCSE 203

Query: 234 -------------------------------REKLKKLLDAVNDVTGKEDLISYLRMLSL 293
                                           +KLK+L  +   +T K+ L+  LR   +
Sbjct: 204 MLERLEVTDSDSPGSSDKMYQSTCSHPPDMHTQKLKQLFASAKTLTAKQQLLHTLRSHLI 263

Query: 294 QKIAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARW-EVPVVLPLLDC 353
             IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V ++ P+ + 
Sbjct: 264 LHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVDIIRPMREY 323

Query: 354 LAQEL---NMLCSVDGLKTVELTKKSS--SGINDLVSSFVALLQVENPSRESTIVRTAGK 413
            ++E+   N   +V  +    L  K+S  S I  L   FV  LQ + PS  ST+ RT+ K
Sbjct: 324 SSKEIAYYNRFFNVSPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVSTLYRTSEK 383

Query: 414 LTPFNFSRIPDLNESNN--------VPLATKRRQ----QRNNFKH---------VNSLSS 470
           L   N S+I    E+           PL T+  +          H          N L++
Sbjct: 384 L---NVSKIDADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIPMKSNDLAN 443

[Close]

BLAST of Chy4G084170 vs. ExPASy Swiss-Prot
Match: Q32NV1 (Cytoplasmic tRNA 2-thiolation protein 2-B OS=Xenopus laevis OX=8355 GN=ctu2-b PE=2 SV=2)

HSP 1 Score: 113.2 bits (282), Expect = 7.8e-24
Identity = 134/514 (26.07%), Postives = 211/514 (41.05%), Query Frame = 0


Query: 54  LCFKCKSKERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPC 113
           +C KCK         AGD   FC  CF+     KFR  +  N +I P +KVL+A+SGGP 
Sbjct: 24  ICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAYSGGPS 83

Query: 114 SRVALQFVHEMQVKAHNNFEASRDRSLQV-FGVGVAYINETAACSIPSDEIDKTIQTIQS 173
           S   ++ V E           SRD   ++ F  G+ +I+E  AC +  +E     Q +  
Sbjct: 84  SSAMVRQVQE---------GLSRDAPKKLRFVPGILFIDEGTACGMSWEERQ---QILSE 143

Query: 174 VVSSLCQPRKNLHIVQIESVYS---SNLSDG----------------------------- 233
           + S L Q +   HIV +E V+S   S L  G                             
Sbjct: 144 ICSVLQQTKIPFHIVSLEQVFSLPGSVLQRGAPEQRPNYKEEVDRFLVQEREQGDAGCSE 203

Query: 234 -------------------------------REKLKKLLDAVNDVTGKEDLISYLRMLSL 293
                                           +KLK+L  +   +T K+ L+  LR   +
Sbjct: 204 MLERLEVTDSDSPGSSDKMYQSTCSRPPDMHTQKLKQLFASAKTLTAKQQLLHTLRSHLI 263

Query: 294 QKIAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARW-EVPVVLPLLDC 353
             IA   GY++++ G   +R++  ++S+   G+G  LP D  + D+R+ +V ++ P+ + 
Sbjct: 264 LHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDTGFCDSRYGDVDIIRPMREY 323

Query: 354 LAQEL---NMLCSVDGLKTVELTKKSS--SGINDLVSSFVALLQVENPSRESTIVRTAGK 413
            ++E+   N   +V  +    L  K+S  S I  L   FV  LQ + PS  ST+ RT+ K
Sbjct: 324 SSKEIAYYNRFFNVLPIFIPALDTKASENSSIQHLTEVFVNRLQADFPSTVSTLYRTSEK 383

Query: 414 LTPFNFSRIPDLNESNN--------VPLATKRRQ----QRNNFKH---------VNSLSS 470
           L   N S I    E+           PL T+  +          H          N L++
Sbjct: 384 L---NVSIIDADQETCAKDRCLLCLSPLDTQAGKASAFSATQLSHHLSQKIPMKSNDLAN 443

[Close]

BLAST of Chy4G084170 vs. ExPASy TrEMBL
Match: A0A0A0LC57 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G816060 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 455/469 (97.01%), Postives = 459/469 (97.87%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIE+DSPPSSRPDT AA+EARRVIT DDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSG GDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKT+QTIQSVVSSLCQP
Sbjct: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RKNLHIVQIESVYSSNL+DGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLP DVQYIDARWEVPVVLPLLDCLAQELNMLC VD
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSISGACCSSCQFQIL 420
           VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDV TRHA SSI GACCSSCQFQIL
Sbjct: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES 470
           PKDSSSQELFLSLLP SMVARATHG LGNSNSLRKKIQEFLLSDDEAES
Sbjct: 421 PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 469

[Close]

BLAST of Chy4G084170 vs. ExPASy TrEMBL
Match: A0A1S3B7H9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 PE=3 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 5.5e-246
Identity = 442/469 (94.24%), Postives = 451/469 (96.16%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIE+DSPPSSRP TAA  E+R  ITNDDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSG GDDGR CSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHE+QVKAH NFEASRDRSLQVFGVGVAYINETAA SIPSDEIDKTIQTIQSVVSSL QP
Sbjct: 121 VHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIV IE+VYSSNLSDGREKLKKLLDAV+DVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLC +D
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCID 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSISGACCSSCQFQIL 420
           VPLATKRRQ+RNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSI GACCSSCQFQIL
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES 470
           PKDS S ELFLSLLP SMVARATHG LGNSNSLR KIQEFLLSDD+AES
Sbjct: 421 PKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

[Close]

BLAST of Chy4G084170 vs. ExPASy TrEMBL
Match: A0A5D3DPK4 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006240 PE=3 SV=1)

HSP 1 Score: 859.8 bits (2220), Expect = 5.5e-246
Identity = 442/469 (94.24%), Postives = 451/469 (96.16%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIE+DSPPSSRP TAA  E+R  ITNDDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSG GDDGR CSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHE+QVKAH NFEASRDRSLQVFGVGVAYINETAA SIPSDEIDKTIQTIQSVVSSL QP
Sbjct: 121 VHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIV IE+VYSSNLSDGREKLKKLLDAV+DVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLC +D
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCID 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSISGACCSSCQFQIL 420
           VPLATKRRQ+RNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSI GACCSSCQFQIL
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES 470
           PKDS S ELFLSLLP SMVARATHG LGNSNSLR KIQEFLLSDD+AES
Sbjct: 421 PKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

[Close]

BLAST of Chy4G084170 vs. ExPASy TrEMBL
Match: A0A6J1FHP7 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445447 PE=3 SV=1)

HSP 1 Score: 755.7 bits (1950), Expect = 1.1e-214
Identity = 393/475 (82.74%), Postives = 420/475 (88.42%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+E+DS  +++ D  AA       TN D+ +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+S  GDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHEMQVKAH NFEASRDRSLQVFGVGVAYI+ET+AC IP  EID+TIQTIQSVVSSL  P
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNLSDGREKLKKLLD V+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           L++GTCTSRIACHVISSTVKGQGYSLPAD+QY+DARWEVPV LPL DCLAQELNMLC  D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKT ELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN 
Sbjct: 301 GLKTEELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHA------SSSISGACCSS 420
           VPLATKRRQ+RNNFKHVNSLSSE+FCAVCNGPLN SDVQT  +      SS ++G CCSS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSS 420

Query: 421 CQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES 470
           CQFQILPKDS SQEL LSLLP SMVARA HG   NSNSLR++IQEFLLSDDE ES
Sbjct: 421 CQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

[Close]

BLAST of Chy4G084170 vs. ExPASy TrEMBL
Match: A0A6J1I684 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470013 PE=3 SV=1)

HSP 1 Score: 754.6 bits (1947), Expect = 2.5e-214
Identity = 393/475 (82.74%), Postives = 419/475 (88.21%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+E+DS  +++ D  AA       T  D+ +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAA-------TYGDSGDTHLCFKCKA 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+S  GDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHEMQVKAH NFEASRDRSL VFGVGVAYI+ET+AC IP  EID+TIQTIQSVVSSL  P
Sbjct: 121 VHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNLSDGREKLKKLLD V+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           L++GTCTSRIACHVISSTVKGQGYSLPAD+QY+DARWEVPV LPL DCLAQELNMLC  D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN 
Sbjct: 301 GLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHA------SSSISGACCSS 420
           VPLATKRRQ+RNNFKHVNSLSSE+FCAVCNGPLN SDVQT  +      SS ++G CCSS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSS 420

Query: 421 CQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES 470
           CQFQILPKDS SQEL LSLLP SMVARA HG   NSNSLR+KIQEFLLSDDE ES
Sbjct: 421 CQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREKIQEFLLSDDEVES 468

[Close]

BLAST of Chy4G084170 vs. NCBI nr
Match: XP_004136614.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_011652154.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_031737791.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >KGN59393.1 hypothetical protein Csa_001562 [Cucumis sativus])

HSP 1 Score: 898 bits (2320), Expect = 0.0
Identity = 455/469 (97.01%), Postives = 459/469 (97.87%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIE+DSPPSSRPDT AA+EARRVIT DDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPDTPAAMEARRVITKDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSG GDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKT+QTIQSVVSSLCQP
Sbjct: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTVQTIQSVVSSLCQP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RKNLHIVQIESVYSSNL+DGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKNLHIVQIESVYSSNLNDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLP DVQYIDARWEVPVVLPLLDCLAQELNMLC VD
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPGDVQYIDARWEVPVVLPLLDCLAQELNMLCCVD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSISGACCSSCQFQIL 420
           VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDV TRHA SSI GACCSSCQFQIL
Sbjct: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVLTRHAGSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES 469
           PKDSSSQELFLSLLP SMVARATHG LGNSNSLRKKIQEFLLSDDEAES
Sbjct: 421 PKDSSSQELFLSLLPPSMVARATHGSLGNSNSLRKKIQEFLLSDDEAES 469

[Close]

BLAST of Chy4G084170 vs. NCBI nr
Match: XP_008443191.1 (PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >XP_008443192.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >KAA0053926.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo var. makuwa] >TYK25478.1 cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 862 bits (2227), Expect = 2.47e-314
Identity = 442/469 (94.24%), Postives = 451/469 (96.16%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIE+DSPPSSRP TAA  E+R  ITNDDNSNTNLCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESR--ITNDDNSNTNLCFKCKS 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMSTSG GDDGR CSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHE+QVKAH NFEASRDRSLQVFGVGVAYINETAA SIPSDEIDKTIQTIQSVVSSL QP
Sbjct: 121 VHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDEIDKTIQTIQSVVSSLSQP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIV IE+VYSSNLSDGREKLKKLLDAV+DVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLC +D
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCCID 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN
Sbjct: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSISGACCSSCQFQIL 420
           VPLATKRRQ+RNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSI GACCSSCQFQIL
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHASSSILGACCSSCQFQIL 420

Query: 421 PKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES 469
           PKDS S ELFLSLLP SMVARATHG LGNSNSLR KIQEFLLSDD+AES
Sbjct: 421 PKDSLSHELFLSLLPPSMVARATHGSLGNSNSLRNKIQEFLLSDDDAES 467

[Close]

BLAST of Chy4G084170 vs. NCBI nr
Match: XP_038905927.1 (cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida])

HSP 1 Score: 818 bits (2112), Expect = 1.08e-296
Identity = 420/474 (88.61%), Postives = 442/474 (93.25%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQHDEHTIE+ SPPSSR DTAAA+E+ +V TNDD+S+T+LCFKCKS
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQVSPPSSRSDTAAAVESCQVDTNDDSSHTHLCFKCKS 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KER+S+SG GDDGR CSDCFRSNLF KFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERISSSGVGDDGRLCSDCFRSNLFGKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHEMQVKAH NFEAS+DRSLQVFGVGVAYINETAA SIPS EID+TIQTIQSVVSSLC P
Sbjct: 121 VHEMQVKAHKNFEASKDRSLQVFGVGVAYINETAAYSIPSHEIDQTIQTIQSVVSSLCPP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
           RK+LHIVQIE+VYSSNLSDGREKLKKLLD VNDVTGKEDLISYLRMLSLQKIAAENGYNR
Sbjct: 181 RKDLHIVQIENVYSSNLSDGREKLKKLLDTVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           LLVGTCTSRIACHVISSTVKGQGYSLPAD+QYIDARWE+PVVLPLLDCLAQELN+LC VD
Sbjct: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADIQYIDARWEIPVVLPLLDCLAQELNLLCCVD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTK+SSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDL ES+N
Sbjct: 301 GLKTVELTKQSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLTESSN 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRH------ASSSISGA-CCS 420
           VPLATKRRQ+RNNFKHVNS+SSESFCAVCNGPLNTSDVQT         SSSI G  CCS
Sbjct: 361 VPLATKRRQKRNNFKHVNSISSESFCAVCNGPLNTSDVQTLRRFEAGQTSSSILGCTCCS 420

Query: 421 SCQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEA 467
           SCQFQILPKDS SQELFLSLLP SMVARA HG  GNSNSLR++IQEFLLSDDEA
Sbjct: 421 SCQFQILPKDSLSQELFLSLLPPSMVARAMHGSFGNSNSLREQIQEFLLSDDEA 474

[Close]

BLAST of Chy4G084170 vs. NCBI nr
Match: XP_022939604.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata])

HSP 1 Score: 758 bits (1956), Expect = 4.92e-273
Identity = 393/475 (82.74%), Postives = 420/475 (88.42%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSSACQSNCYRNQ DE T+E+DS  +++ D  AA       TN D+ +T+LCFKCK+
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+S  GDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHEMQVKAH NFEASRDRSLQVFGVGVAYI+ET+AC IP  EID+TIQTIQSVVSSL  P
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQSVVSSLSPP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNLSDGREKLKKLLD V+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           L++GTCTSRIACHVISSTVKGQGYSLPAD+QY+DARWEVPV LPL DCLAQELNMLC  D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKT ELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN 
Sbjct: 301 GLKTEELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHA------SSSISGACCSS 420
           VPLATKRRQ+RNNFKHVNSLSSE+FCAVCNGPLN SDVQT  +      SS ++G CCSS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSS 420

Query: 421 CQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES 469
           CQFQILPKDS SQEL LSLLP SMVARA HG   NSNSLR++IQEFLLSDDE ES
Sbjct: 421 CQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

[Close]

BLAST of Chy4G084170 vs. NCBI nr
Match: XP_023539180.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 756 bits (1953), Expect = 1.41e-272
Identity = 392/475 (82.53%), Postives = 419/475 (88.21%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSS CQSNCYRNQ DE T+E+DS  +++ D  AA       TN D+ +T+LCFKCK+
Sbjct: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60

Query: 61  KERMSTSGAGDDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQF 120
           KERMS+S  GDDGRFCSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSRVALQF
Sbjct: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRVALQF 120

Query: 121 VHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQP 180
           VHEMQVKAH NFEASRDRSLQVFGVGVAYI+ET+AC IP  EID+TIQ IQSVVSSL  P
Sbjct: 121 VHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQAIQSVVSSLSPP 180

Query: 181 RKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYNR 240
            K+LHIVQIESVYSSNLSDGREKLKKLLD V+DVTGKEDLIS+LRMLSLQKIAAENGYNR
Sbjct: 181 IKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNR 240

Query: 241 LLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSVD 300
           L++GTCTSRIACHVISSTVKGQGYSLPAD+QY+DARWEVPV LPL DCLAQELNMLC  D
Sbjct: 241 LVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCAD 300

Query: 301 GLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESNN 360
           GLKTVELTK S SGINDLVSSFVALLQ ENPSRESTIVRTAGKLTPFNFSRIPDLNESN 
Sbjct: 301 GLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNG 360

Query: 361 VPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSDVQTRHA------SSSISGACCSS 420
           VPLATKRRQ+RNNFKHVNSLSSE+FCAVCNGPLN SDVQT  +      SS ++G CCSS
Sbjct: 361 VPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSS 420

Query: 421 CQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDEAES 469
           CQFQILPKDS SQEL LSLLP SMVARA HG   NSNSLR++IQEFLLSDDE ES
Sbjct: 421 CQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES 468

[Close]

BLAST of Chy4G084170 vs. TAIR 10
Match: AT4G35910.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 495.0 bits (1273), Expect = 6.8e-140
Identity = 261/473 (55.18%), Postives = 340/473 (71.88%), Query Frame = 0


Query: 1   MACNSSACQSNCYRNQHDEHTIERDSPPSSRPDTAAALEARRVITNDDNSNTNLCFKCKS 60
           MACNSS C+S CY  + D  +   D   S                    ++ ++C KCK 
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDDAVSG-----------------GGNHESVCVKCKC 60

Query: 61  KERMSTSGAG-DDGRFCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSRVALQ 120
              M+    G DDGRFC+DCFR+N+F KFR AVTS+AMI+PSD VLVAFSGG  SRV+LQ
Sbjct: 61  NAPMTFGDGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGGSSSRVSLQ 120

Query: 121 FVHEMQVKAHNNFEASRDRSLQVFGVGVAYINETAACSIPSDEIDKTIQTIQSVVSSLCQ 180
           FVHE+Q+KA  N+EASRDRSL VFGVGVA+++ETAA    S E+   I+ ++  VS L  
Sbjct: 121 FVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDAIEWVRYTVSCLSP 180

Query: 181 PRKNLHIVQIESVYSSNLSDGREKLKKLLDAVNDVTGKEDLISYLRMLSLQKIAAENGYN 240
           P K+LH+V +ES++ S+  D R++L KLLD+V D TGKEDL+ +L+MLSLQK+AAENGYN
Sbjct: 181 PAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKMLSLQKVAAENGYN 240

Query: 241 RLLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVVLPLLDCLAQELNMLCSV 300
           RL++G+CTSRIA HV+++TVKG+GYSL AD+Q++DARW+VP+VLPL DC+  E+  LC +
Sbjct: 241 RLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLRDCVRLEITRLCLL 300

Query: 301 DGLKTVELTKKSSSGINDLVSSFVALLQVENPSRESTIVRTAGKLTPFNFSRIPDLNESN 360
           DGLKTVEL  +S  GINDLVSSFVALLQ ENPSRE TIVRTA KLTPF F++IP+ ++S 
Sbjct: 301 DGLKTVELACRSQCGINDLVSSFVALLQEENPSRECTIVRTAAKLTPFYFNKIPETDDS- 360

Query: 361 NVPLATKRRQQRNNFKHVNSLSSESFCAVCNGPLNTSD------VQTRHASSSISGACCS 420
           NVP+AT+RR +R N K+  S+++E+FC +CNGPLN SD       +    S  +  ACCS
Sbjct: 361 NVPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLNRSDSSELDTFEEGQESDVLYAACCS 420

Query: 421 SCQFQILPKDSSSQELFLSLLPTSMVARATHGCLGNSNSLRKKIQEFLLSDDE 467
           SC+FQILP+D SS E F S LP  M+++  H  + +   LR+KI++ LL DDE
Sbjct: 421 SCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVDSQAYLREKIKDCLLLDDE 455

The following BLAST results are available for this feature:

BLAST of Chy4G084170 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O65628	9.6e-139	55.18	Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 ...	[more]
Q2QMW0	1.7e-103	45.15	Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947...	[more]
A2ZM73	3.8e-103	45.15	Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 G...	[more]
Q08B12	1.4e-25	26.26	Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE...	[more]
Q32NV1	7.8e-24	26.07	Cytoplasmic tRNA 2-thiolation protein 2-B OS=Xenopus laevis OX=8355 GN=ctu2-b PE...	[more]

BLAST of Chy4G084170 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LC57	1.2e-256	97.01	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis sativus OX=3659 GN=Csa_3G8160...	[more]
A0A1S3B7H9	5.5e-246	94.24	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 ...	[more]
A0A5D3DPK4	5.5e-246	94.24	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1FHP7	1.1e-214	82.74	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1I684	2.5e-214	82.74	Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470...	[more]

BLAST of Chy4G084170 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004136614.1	0.0	97.01	cytoplasmic tRNA 2-thiolation protein 2 [Cucumis sativus] >XP_011652154.1 cytopl...	[more]
XP_008443191.1	2.47e-314	94.24	PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cucumis melo] >XP_008443192....	[more]
XP_038905927.1	1.08e-296	88.61	cytoplasmic tRNA 2-thiolation protein 2 [Benincasa hispida]	[more]
XP_022939604.1	4.92e-273	82.74	cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata]	[more]
XP_023539180.1	1.41e-272	82.53	cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy4G084170 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35910.1	6.8e-140	55.18	Adenine nucleotide alpha hydrolases-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 54..413 e-value: 8.7E-42 score: 145.2
IPR019407	Cytoplasmic tRNA 2-thiolation protein 2	PANTHER	PTHR20882	CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2	coord: 48..463
IPR019407	Cytoplasmic tRNA 2-thiolation protein 2	HAMAP	MF_03054	CTU2	coord: 49..423 score: 20.557703
None	No IPR available	SUPERFAMILY	52402	Adenine nucleotide alpha hydrolases-like	coord: 78..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G084170.1	Chy4G084170.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010311	lateral root formation
biological_process	GO:0032447	protein urmylation
biological_process	GO:0002143	tRNA wobble position uridine thiolation
biological_process	GO:0034227	tRNA thio-modification
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005829	cytosol
molecular_function	GO:0016779	nucleotidyltransferase activity
molecular_function	GO:0016783	sulfurtransferase activity
molecular_function	GO:0000049	tRNA binding