Chy4G082580 (gene) Cucumber (hystrix) v1

Overview
NameChy4G082580
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionaquaporin NIP2-1-like
LocationchrH04: 22005911 .. 22010159 (-)
RNA-Seq ExpressionChy4G082580
SyntenyChy4G082580
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTCTATTCAAAATCCTGTGCTCTCCAACCAAGAAACTGTTGTGGATGTTAACGAATTCGTATCTGTTGAAAACCCAGATTCTAAACGCTCCAAGTTTGGATCTTTCTTCAAAAACCCTTACCCTCCTGGGTTTTCCCGAAAGGTAATCACCTTCTTGTATATCACTTATCACTTATGTTTACTTTGTTTAAAATCACTCCAAAACCTGCGTTTATCACTAAAGAATCACTTTATTGTTTAATTTTACACGTTTAAATCATTTTTTCATTCTGATATTCAACAAATCAAAACACTTTACAAGTGGTTTTGAATCGACAAAAGAGATTTTGACTCTTAAACATGTCTATCTCAGTAAAAGTCAATTCAACGATTATGATTTTTTTTCTTTTCCAACAATGATATAGCTGGTGGCGGAGGTGATAGCAACCTATTTGCTAGTGTTTGTGACATGTGGGGCGGCAGCATTGAACGCGAGCGATGCACGGAGAGTGTCGCAGCTTGGTGCTTCAGTTGCAGGTGGATTGATCGTGACTGTGATGATTTACGCTGTCGGACACGTTTCCGGTGCCCATATGAACCCGGCTGTCACCATGGCTTTTGCTGCAACTCGACACTTTCCATGGAAACAGGTATCAACTCTTTTATTCTTCAAATGGTTGAAATTGTCAGTAATATTTTCTTCGTTTAATCAATAACCTAATCATTTCTTCCCACATGTTCTCTAAAATTGTAGGGCGATTATGTCCAAGTATTTTCATTTAAAAAAAAAAAAAATTCAATATCTAACTTGTCTACTATTATTATTATTTTTTCTCTAGACTTAAGTACTAGACATAGAGTTTTGTTTTACTTTTGCCATTAATGCTTTTAGGGTAAGAATATTTATTAAGTGATTAATGAAAAATCAATCACGAGACAACACGGAGATTAATACAAACCATTAGAAAATTGATGGATCAAATTTATACTTTTAGAATTAAGGGATCAAATAAAAAGTTAAATAGAATTTTTTTGAGCTTAAATTTTATTTATGACGAATATATTTGTTTTGTTTATTGTTTATTATTATATATTATTTAACTACTTTCGTAAGCTAGCTAATCGATTAAAAATTAAATAATAACTAGTGCACGATTAATTTAATATGTTAATGATATTATATAGCCTTTTTTTTGTAAGGAAAAAGCCAGATAGTTGCTATTTACAAAAGGAAAAAAGGAAGAACTATTAGTTTATAAAATATCATTAGCAAGTAAATATAATATTAGTTAAACTAATGCTATAATTTAATAATGATCAAATCAACGTATTATAAAACTAAATTTGATACCTCATTTGTTTAAAATTTAATATTTAATATTTAGAGCGGTGCTAAGAATATAGGAACGAGGAGCACCTTGTTCTTTCCATGACTTCAAAATATATTTATATATCAATTTTAAATTGGGGGTTTCAAGTAATCGTCTAACTTTCTTCCAATTTATTAACTTTCTTTTTTAAAAAAACTACTAATCTATATTGTCTCGTGATATATAGGATTCTTCACCCTCCAATTATTATAAAAAATAGAATTAAATATTGTTCAAGTTGAGAATAACCTGTAAATATAGCAAATACAATCCAATAACTGTCTAGGGTTGAAGTATCACAAGGCTTTTAAAAATATGTCGATTTTTCAGATTTTTTTCTACTGATCCCCATTGTTTTTTAATATATTTCTTTAAAAACATTAGTCAAAAGTTTATTGTTGGAAAGAAATAAAAATGTGAGGTGAAAAATGAAAGCACACGAGAGCCATTAACCATGGTTTGGACTATTTATTATGACATTTAAGAGAAGAATTTGGTGGGATTTAAAATTGACCCTTAGCTTCTTCTAATAATTAGTTAGAAAGGAAGTCTAGAAGAATCACTATGTACATATAGGAAAAGGAGTAGAAATGAAATGATGATGAGATAACAAAAAGAGGTAAATGTAAGCCATATTTAGTGGCCACATGATGTAGGGTCAAGGGTTGTCCAAATATTATTCTACATCCATAGCCACAAGGACAAGCATATGTGATCTTTTTATGGCCACAACCTTGGCTTCATTTTGCCGACCAAGGAATTAGATGGGACATTTAGGCAGCATAATTCCAACACTTGAGAAATAGAAGAGATAGAGATAGAGATGTCCACCAAAAAGTTCTTGGCCCATTAGAAGTGGGAAGGTATAGAGAGAGAGCAAAAAAGAACAATACATATCTTCATGTCATGTGTGTTTTTATAGTCAAGATGGCCATCTTTTACTTTATAGATATATTGATATTTCACTTTTTGGAATTCCAATCAAAGCATTCAAAAGTAATAATTTTAAATGTCTAGTCTTGATCTTTTTGATTTTGGTTTAGGTTTTTTTAGGAGGGATTTTTTTGTCTCAACACACTTTAATAATTCTTGGTTTGATTGAGTAGGTTTTGTTAAAGATGTAGATCATATCTAAGTTTATGGGTAAGTCATGCTTGAAGTTTGTGTGTTTAAAACTACATGTAGAATTAATAAGTTTAGTTTGCCAGTTTTTCCACCATTTAATTAAAGTGTCCTTTGTTGTTATTTTGTGTGAAAAAAAATATTTCATCACACAAATCTATGAAACAAAAATCTAATTTGTATTAAACGTGATAAACAAAATTCCATGGTTAAAATTTATATCTATATATAAAAAAAGATGTAGGTCACGAGATAGAGCTAGCAAAATTCGAATTGAAATGAAATTGAATTAACAAAGATATATAAAGATATATAGTAAAAGACGTATTTAACATAACTAGAGAATGCATCATTTTTTGAGTTGTCTCCGTCTATCCACTAATTTAATCTCTTCTAGTATTCTGTTTTTTTTTTTAAAAAAAAAAGCATAATAAATAGGGGATGAAAGTATAGAATTCAAATGATTGTAAAGACAACGCAATAATTTATTTTGATGGATGCATTAATGAGATGTCAAAAATACATGTAGTTTTGTGAATTGAATTGATTGGGAAAGAAAAAGGGAATTTGGAAGATGATTGAAAGTAACACATAAAAGAGAGTGATGTACTATATTGGGTTTGATTGGTAGGTTCCATTGTATGGAGCAGCACAATTGAGTGGAGCAACTTGTGCAGCCTTTACATTGCGCTTATTATTGCATCCAATTAAGCATTTGGGCACAACTACTCCATCAGGGTCTGATTTACAAGCTTTAGTTATGGAGATTGTTGTTACATTCTCTATGATGTTTGTCACTCTTGCCGTTGCAACTGACACCAAAGCAGTAAGATTCTTTCACTTATACCTAAATATATCCCTTTAAATCAAAACTACTTTATTTATTTTAAGTCTTGAAAATTATAGGGAAAACTATTTTTAGAAAATGGATCTGTAATGAAATATGGATAAAAGTAAGTGGGAGCTAGGTTGGAGTCCCCACTCAACATTTAAAAAATGAAAATAAAGATAGAATGTAAATATTTAGAATGCAAAGAAATTAAATGAATGCAGTGAATAATTAATTCAATGTGTTTTTATTAATGTGGGAAAATGGTTGTTTTTGCTTTGGTTTGAGTTTGGCAGGTAGGAGAGCTTGCAGGTATTGCAGTAGGCTCAGCTGTTTGTATCACTTCCATCTTAGCTGGGTGAGTGAGGAAAATATAACATTTTTGGTTTTCCCTTTTTAACACACAAAATTAAAATTCTTTGCTTCAAAATATATTATAATAATAATAATCATAATTAAAAAAACATATTCTTATTTATTTTATTTTTTTCAAAAATACCCTTATTTTCTACATGTTAATACTTTATTGCAAATGTTTAACTTTACAAAACCATGCATGCATAGCAACAATTTTGATTTTAAAAAGTTCAATTTTTGGGAACTTTTTTTTTGTAAACCTCCGTTTTAAAAGAAAATGTTTTGAAAATATAAAGATTTTACATTAAACAAATAGATCTAATAAGCATTTGTGTTAGCAACTATTCAGACCTGTATCAGGAGGGTCAATGAACCCTGTGAGGACTTTAGGACCTGCATTGGCAAGTGATTATTATAAAGGGCTTTGGGTGTACTTTGTTGGGCCGGTTGTTGGAACTCAACTGGGGGCATGGTCATATAAGTTCATACGTGCCAGTGACAAACCTGTGCACTTAATTTCTCCTCATTCTTTTTCACTAAAAATGCGAAGAATGTCGAGATCAGATGTTAGCGAAAGCAATCATTAA

mRNA sequence

ATGAGTTCTATTCAAAATCCTGTGCTCTCCAACCAAGAAACTGTTGTGGATGTTAACGAATTCGTATCTGTTGAAAACCCAGATTCTAAACGCTCCAAGTTTGGATCTTTCTTCAAAAACCCTTACCCTCCTGGGTTTTCCCGAAAGCTGGTGGCGGAGGTGATAGCAACCTATTTGCTAGTGTTTGTGACATGTGGGGCGGCAGCATTGAACGCGAGCGATGCACGGAGAGTGTCGCAGCTTGGTGCTTCAGTTGCAGGTGGATTGATCGTGACTGTGATGATTTACGCTGTCGGACACGTTTCCGGTGCCCATATGAACCCGGCTGTCACCATGGCTTTTGCTGCAACTCGACACTTTCCATGGAAACAGGTTCCATTGTATGGAGCAGCACAATTGAGTGGAGCAACTTGTGCAGCCTTTACATTGCGCTTATTATTGCATCCAATTAAGCATTTGGGCACAACTACTCCATCAGGGTCTGATTTACAAGCTTTAGTTATGGAGATTGTTGTTACATTCTCTATGATGTTTGTCACTCTTGCCGTTGCAACTGACACCAAAGCAGTAGGAGAGCTTGCAGGTATTGCAGTAGGCTCAGCTGTTTGTATCACTTCCATCTTAGCTGGACCTGTATCAGGAGGGTCAATGAACCCTGTGAGGACTTTAGGACCTGCATTGGCAAGTGATTATTATAAAGGGCTTTGGGTGTACTTTGTTGGGCCGGTTGTTGGAACTCAACTGGGGGCATGGTCATATAAGTTCATACGTGCCAGTGACAAACCTGTGCACTTAATTTCTCCTCATTCTTTTTCACTAAAAATGCGAAGAATGTCGAGATCAGATGTTAGCGAAAGCAATCATTAA

Coding sequence (CDS)

ATGAGTTCTATTCAAAATCCTGTGCTCTCCAACCAAGAAACTGTTGTGGATGTTAACGAATTCGTATCTGTTGAAAACCCAGATTCTAAACGCTCCAAGTTTGGATCTTTCTTCAAAAACCCTTACCCTCCTGGGTTTTCCCGAAAGCTGGTGGCGGAGGTGATAGCAACCTATTTGCTAGTGTTTGTGACATGTGGGGCGGCAGCATTGAACGCGAGCGATGCACGGAGAGTGTCGCAGCTTGGTGCTTCAGTTGCAGGTGGATTGATCGTGACTGTGATGATTTACGCTGTCGGACACGTTTCCGGTGCCCATATGAACCCGGCTGTCACCATGGCTTTTGCTGCAACTCGACACTTTCCATGGAAACAGGTTCCATTGTATGGAGCAGCACAATTGAGTGGAGCAACTTGTGCAGCCTTTACATTGCGCTTATTATTGCATCCAATTAAGCATTTGGGCACAACTACTCCATCAGGGTCTGATTTACAAGCTTTAGTTATGGAGATTGTTGTTACATTCTCTATGATGTTTGTCACTCTTGCCGTTGCAACTGACACCAAAGCAGTAGGAGAGCTTGCAGGTATTGCAGTAGGCTCAGCTGTTTGTATCACTTCCATCTTAGCTGGACCTGTATCAGGAGGGTCAATGAACCCTGTGAGGACTTTAGGACCTGCATTGGCAAGTGATTATTATAAAGGGCTTTGGGTGTACTTTGTTGGGCCGGTTGTTGGAACTCAACTGGGGGCATGGTCATATAAGTTCATACGTGCCAGTGACAAACCTGTGCACTTAATTTCTCCTCATTCTTTTTCACTAAAAATGCGAAGAATGTCGAGATCAGATGTTAGCGAAAGCAATCATTAA

Protein sequence

MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVTLAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH*
Homology
BLAST of Chy4G082580 vs. ExPASy Swiss-Prot
Match: Q19KC1 (Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2)

HSP 1 Score: 321.2 bits (822), Expect = 1.2e-86
Identity = 164/269 (60.97%), Postives = 207/269 (76.95%), Query Frame = 0

Query: 17  DVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAAALNASDAR 76
           ++++  + +N     + +     + +PP   +K+V+EV++T+LLVFVTCGAA +  SD  
Sbjct: 16  EIHDIGTAQNSSMPPTYYDRSLADIFPPHLLKKVVSEVVSTFLLVFVTCGAAGIYGSDKD 75

Query: 77  RVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHFPWKQVPLYGAAQLSGA 136
           R+SQLG SVAGGLIVTVMIYAVGH+SGAHMNPAVT+AFA  RHFPW QVP Y AAQ +G+
Sbjct: 76  RISQLGQSVAGGLIVTVMIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTGS 135

Query: 137 TCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVTLAVATDTKAVGELAGI 196
            CA+F L+ +LHPI  LGTTTP+G    +LV+EI+VTF+MMFVTLAVATDT+AVGELAG+
Sbjct: 136 ICASFVLKAVLHPIAVLGTTTPTGPHWHSLVIEIIVTFNMMFVTLAVATDTRAVGELAGL 195

Query: 197 AVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFI 256
           AVGSAVCITSI AG VSGGSMNP RTLGPALAS+ Y GLW+YF+GPV+GT  GAW+Y +I
Sbjct: 196 AVGSAVCITSIFAGAVSGGSMNPARTLGPALASNLYTGLWIYFLGPVLGTLSGAWTYTYI 255

Query: 257 RASDKPVHL-ISPHSFSLKMRRMSRSDVS 285
           R  + P H  +S    S K+RR+    V+
Sbjct: 256 RFEEAPSHKDMSQKLSSFKLRRLQSQSVA 284

BLAST of Chy4G082580 vs. ExPASy Swiss-Prot
Match: Q6Z2T3 (Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.7e-85
Identity = 160/263 (60.84%), Postives = 204/263 (77.57%), Query Frame = 0

Query: 17  DVNEFVSVENPDSKRSKFG-SFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAAALNASDA 76
           ++++  +V+N       +G     + +PP   +K+V+EV+AT+LLVF+TCGAA ++ SD 
Sbjct: 17  EIHDLSTVQNGTMPTMYYGEKAIADFFPPHLLKKVVSEVVATFLLVFMTCGAAGISGSDL 76

Query: 77  RRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHFPWKQVPLYGAAQLSG 136
            R+SQLG S+AGGLIVTVMIYAVGH+SGAHMNPAVT+AFA  RHFPW QVP Y AAQ +G
Sbjct: 77  SRISQLGQSIAGGLIVTVMIYAVGHISGAHMNPAVTLAFAVFRHFPWIQVPFYWAAQFTG 136

Query: 137 ATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVTLAVATDTKAVGELAG 196
           A CA+F L+ ++HP+  +GTTTP G    +LV+E++VTF+MMFVTLAVATDT+AVGELAG
Sbjct: 137 AICASFVLKAVIHPVDVIGTTTPVGPHWHSLVVEVIVTFNMMFVTLAVATDTRAVGELAG 196

Query: 197 IAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKF 256
           +AVGSAVCITSI AG +SGGSMNP RTLGPALAS+ + GLW+YF+GPV+GT  GAW+Y F
Sbjct: 197 LAVGSAVCITSIFAGAISGGSMNPARTLGPALASNKFDGLWIYFLGPVMGTLSGAWTYTF 256

Query: 257 IRASDKPVHLISPHSFSLKMRRM 279
           IR  D P    S    S K+RR+
Sbjct: 257 IRFEDTPKEGSSQKLSSFKLRRL 279

BLAST of Chy4G082580 vs. ExPASy Swiss-Prot
Match: Q9ATN2 (Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.2e-85
Identity = 158/237 (66.67%), Postives = 191/237 (80.59%), Query Frame = 0

Query: 42  YPPGFSRKLVAEVIATYLLVFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHV 101
           +PP   +K+++EV+AT+LLVFVTCGAA++   D RR+SQLG SVAGGLIVTVMIYA GH+
Sbjct: 46  FPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNRRISQLGQSVAGGLIVTVMIYATGHI 105

Query: 102 SGAHMNPAVTMAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGS 161
           SGAHMNPAVT++FA  RHFPW QVP Y AAQ +GA CAAF L+ +LHPI  +GTTTPSG 
Sbjct: 106 SGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAVLHPIAVIGTTTPSGP 165

Query: 162 DLQALVMEIVVTFSMMFVTLAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVR 221
              AL++EIVVTF+MMFVT AVATD++AVGELAG+AVGSAVCITSI AGPVSGGSMNP R
Sbjct: 166 HWHALLIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITSIFAGPVSGGSMNPAR 225

Query: 222 TLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRM 279
           TL PA+AS+ + GLW+YF+GPV+GT  GAW Y +IR  + P    +    S K+RRM
Sbjct: 226 TLAPAVASNVFTGLWIYFLGPVIGTLSGAWVYTYIRFEEAPAAKDTQRLSSFKLRRM 282

BLAST of Chy4G082580 vs. ExPASy Swiss-Prot
Match: Q67WJ8 (Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.1e-84
Identity = 166/274 (60.58%), Postives = 204/274 (74.45%), Query Frame = 0

Query: 14  TVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAAALNAS 73
           TV  V+   SV  P+         F + +PP   +K+++EV+AT+LLVFVTCGAA++   
Sbjct: 25  TVQSVSAVPSVYYPEKS-------FADIFPPNLLKKVISEVVATFLLVFVTCGAASIYGE 84

Query: 74  DARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHFPWKQVPLYGAAQL 133
           D +R+SQLG SV GGLIVTVMIYA GH+SGAHMNPAVT++FA  RHFPW QVP Y AAQ 
Sbjct: 85  DMKRISQLGQSVVGGLIVTVMIYATGHISGAHMNPAVTLSFAFFRHFPWIQVPFYWAAQF 144

Query: 134 SGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVTLAVATDTKAVGEL 193
           +GA CAAF LR +L+PI+ LGTTTP+G    ALV+EIVVTF+MMFVT AVATD++AVGEL
Sbjct: 145 TGAMCAAFVLRAVLYPIEVLGTTTPTGPHWHALVIEIVVTFNMMFVTCAVATDSRAVGEL 204

Query: 194 AGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSY 253
           AG+AVGSAVCITSI AGPVSGGSMNP RTL PA+AS+ Y GLW+YF+GPVVGT  GAW Y
Sbjct: 205 AGLAVGSAVCITSIFAGPVSGGSMNPARTLAPAVASNVYTGLWIYFLGPVVGTLSGAWVY 264

Query: 254 KFIRASDKPVHL--ISPHSF-SLKMRRMSRSDVS 285
            +IR  + P      +P    S K+RR+    ++
Sbjct: 265 TYIRFEEAPAAAGGAAPQKLSSFKLRRLQSQSMA 291

BLAST of Chy4G082580 vs. ExPASy Swiss-Prot
Match: Q9AT74 (Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 3.0e-82
Identity = 161/254 (63.39%), Postives = 193/254 (75.98%), Query Frame = 0

Query: 27  PDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAAALNASDARRVSQLGASVA 86
           PDSK         + +PP   +K+++EV+AT+LLVFVTCGAA++   D  R+SQLG SVA
Sbjct: 40  PDSKS------IADIFPPHLGKKVISEVVATFLLVFVTCGAASIYGEDNARISQLGQSVA 99

Query: 87  GGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRLL 146
           GGLIVTVMIYA GH+SGAHMNPAVT++FA  RHFPW QVP Y AAQ +GA CAAF L+ +
Sbjct: 100 GGLIVTVMIYATGHISGAHMNPAVTLSFACFRHFPWIQVPFYWAAQFTGAMCAAFVLKAV 159

Query: 147 LHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVTLAVATDTKAVGELAGIAVGSAVCITS 206
           L PI  +GTTTPSG    AL +EIVVTF+MMFVT AVATD++AVGELAG+AVGSAVCITS
Sbjct: 160 LQPIAVIGTTTPSGPHWHALAIEIVVTFNMMFVTCAVATDSRAVGELAGLAVGSAVCITS 219

Query: 207 ILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFIRASDKPVHL- 266
           I AGPVSGGSMNP RTL PA+AS+ + GLW+YF+GPVVGT  GAW Y +IR  + P    
Sbjct: 220 IFAGPVSGGSMNPARTLAPAVASNVFTGLWIYFLGPVVGTLSGAWVYTYIRFEEAPAAAK 279

Query: 267 -ISPHSFSLKMRRM 279
             +    S K+RRM
Sbjct: 280 PDTQRLSSFKLRRM 287

BLAST of Chy4G082580 vs. ExPASy TrEMBL
Match: G8XUV3 (Si transport-like protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G826640 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.3e-153
Identity = 286/288 (99.31%), Postives = 286/288 (99.31%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSSIQNP LSNQE VVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSIQNPQLSNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
           LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 289
           GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH
Sbjct: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 288

BLAST of Chy4G082580 vs. ExPASy TrEMBL
Match: A0A1L7B7J0 (LSi1 OS=Cucumis sativus var. sativus OX=869827 GN=LSi1 PE=2 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.3e-153
Identity = 286/288 (99.31%), Postives = 286/288 (99.31%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSSIQNP LSNQE VVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSIQNPQLSNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
           LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 289
           GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH
Sbjct: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 288

BLAST of Chy4G082580 vs. ExPASy TrEMBL
Match: A0A5D3DQ37 (Aquaporin NIP2-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G008030 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 5.0e-149
Identity = 281/288 (97.57%), Postives = 284/288 (98.61%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSSI NP LSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSI-NPELSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALNASD +RVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDPQRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
           LAVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPALASD YKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDNYKGLWVYFV 240

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 289
           GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLK+RRMSRSDVSESNH
Sbjct: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKLRRMSRSDVSESNH 287

BLAST of Chy4G082580 vs. ExPASy TrEMBL
Match: A0A1S3B807 (aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487002 PE=3 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 5.0e-149
Identity = 281/288 (97.57%), Postives = 284/288 (98.61%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSSI NP LSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSI-NPELSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALNASD +RVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDPQRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
           LAVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPALASD YKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDNYKGLWVYFV 240

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 289
           GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLK+RRMSRSDVSESNH
Sbjct: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKLRRMSRSDVSESNH 287

BLAST of Chy4G082580 vs. ExPASy TrEMBL
Match: Q7X9T0 (Nod26-like protein OS=Cucurbita pepo OX=3663 GN=NIP1 PE=2 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.0e-139
Identity = 261/285 (91.58%), Postives = 270/285 (94.74%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS Q+P L  Q++VVDV EFVSVENPDSKRS+FGS FKN YPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSSQDPQLVQQQSVVDVEEFVSVENPDSKRSQFGSLFKNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALN SDA+RVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVT AFAATRHF
Sbjct: 61  VFVTCGAAALNGSDAQRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTTAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLY AAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYAAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
            AVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPA+ASD YKGLWVYFV
Sbjct: 181 CAVATDTKAVGELAGLAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASDNYKGLWVYFV 240

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSE 286
           GPV GT LGAWSYKFIRASDKPVHLISPHSFSLK+RRMSRSDV E
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSLKLRRMSRSDVGE 285

BLAST of Chy4G082580 vs. NCBI nr
Match: NP_001267699.1 (aquaporin NIP2-1-like [Cucumis sativus] >ACU29603.1 Si transport-like protein 1 [Cucumis sativus] >AOW42547.1 silicon transporter Lsi1 [Cucumis sativus] >APT69293.1 LSi1 [Cucumis sativus var. sativus] >KGN59570.1 hypothetical protein Csa_001739 [Cucumis sativus])

HSP 1 Score: 553 bits (1425), Expect = 3.52e-198
Identity = 286/288 (99.31%), Postives = 286/288 (99.31%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSSIQNP LSNQE VVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSIQNPQLSNQEAVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
           LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 288
           GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH
Sbjct: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 288

BLAST of Chy4G082580 vs. NCBI nr
Match: XP_008443411.1 (PREDICTED: aquaporin NIP2-1-like [Cucumis melo] >KAA0053759.1 aquaporin NIP2-1-like [Cucumis melo var. makuwa] >TYK25642.1 aquaporin NIP2-1-like [Cucumis melo var. makuwa])

HSP 1 Score: 539 bits (1389), Expect = 1.04e-192
Identity = 281/288 (97.57%), Postives = 284/288 (98.61%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSSI NP LSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSI-NPELSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALNASD +RVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF
Sbjct: 61  VFVTCGAAALNASDPQRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
           LAVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPALASD YKGLWVYFV
Sbjct: 181 LAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDNYKGLWVYFV 240

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSESNH 288
           GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLK+RRMSRSDVSESNH
Sbjct: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKLRRMSRSDVSESNH 287

BLAST of Chy4G082580 vs. NCBI nr
Match: XP_038905688.1 (aquaporin NIP2-1-like [Benincasa hispida])

HSP 1 Score: 529 bits (1363), Expect = 7.16e-188
Identity = 270/285 (94.74%), Postives = 279/285 (97.89%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS+QN  L NQETVV+VNEFVSVENPDS+RSKFGSFFKNPYPPGFSRKLVAEVI+TYLL
Sbjct: 55  MSSVQNSELPNQETVVNVNEFVSVENPDSRRSKFGSFFKNPYPPGFSRKLVAEVISTYLL 114

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALNA+D RRVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVTMAFAATRHF
Sbjct: 115 VFVTCGAAALNANDQRRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTMAFAATRHF 174

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSD QALVMEIVVTFSMMFVT
Sbjct: 175 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDFQALVMEIVVTFSMMFVT 234

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
            AVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV
Sbjct: 235 AAVATDTKAVGELAGMAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 294

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSE 285
           GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLK+RRMSRSD+SE
Sbjct: 295 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKLRRMSRSDISE 339

BLAST of Chy4G082580 vs. NCBI nr
Match: XP_023529347.1 (aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo] >KAG7022218.1 Aquaporin NIP2-1 [Cucurbita argyrosperma subsp. argyrosperma] >CAD67694.1 Nod26-like protein [Cucurbita pepo])

HSP 1 Score: 505 bits (1301), Expect = 2.79e-179
Identity = 261/285 (91.58%), Postives = 270/285 (94.74%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS Q+P L  Q++VVDV EFVSVENPDSKRS+FGS FKN YPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSSQDPQLVQQQSVVDVEEFVSVENPDSKRSQFGSLFKNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALN SDA+RVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVT AFAATRHF
Sbjct: 61  VFVTCGAAALNGSDAQRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTTAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLY AAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYAAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
            AVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPA+ASD YKGLWVYFV
Sbjct: 181 CAVATDTKAVGELAGLAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASDNYKGLWVYFV 240

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSE 285
           GPV GT LGAWSYKFIRASDKPVHLISPHSFSLK+RRMSRSDV E
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSLKLRRMSRSDVGE 285

BLAST of Chy4G082580 vs. NCBI nr
Match: XP_022934019.1 (aquaporin NIP2-1-like [Cucurbita moschata] >BAK09175.1 silicon transporter 1 [Cucurbita moschata])

HSP 1 Score: 504 bits (1297), Expect = 1.13e-178
Identity = 260/285 (91.23%), Postives = 269/285 (94.39%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           MSS Q+P L  Q++VVDV EFVSVENPDSKRS+FGS FKN YPPGFSRKLVAEVIATYLL
Sbjct: 1   MSSSQDPQLVQQQSVVDVEEFVSVENPDSKRSQFGSLFKNHYPPGFSRKLVAEVIATYLL 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           VFVTCGAAALN SD +RVSQLGASVAGGLIVTVMIYAVGH+SGAHMNPAVT AFAATRHF
Sbjct: 61  VFVTCGAAALNGSDVQRVSQLGASVAGGLIVTVMIYAVGHISGAHMNPAVTTAFAATRHF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180
           PWKQVPLY AAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT
Sbjct: 121 PWKQVPLYAAAQLSGATCAAFTLRLLLHPIKHLGTTTPSGSDLQALVMEIVVTFSMMFVT 180

Query: 181 LAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFV 240
            AVATDTKAVGELAG+AVGSAVCITSILAGPVSGGSMNPVRTLGPA+ASD YKGLWVYFV
Sbjct: 181 CAVATDTKAVGELAGLAVGSAVCITSILAGPVSGGSMNPVRTLGPAMASDNYKGLWVYFV 240

Query: 241 GPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRRMSRSDVSE 285
           GPV GT LGAWSYKFIRASDKPVHLISPHSFSLK+RRMSRSDV E
Sbjct: 241 GPVTGTLLGAWSYKFIRASDKPVHLISPHSFSLKLRRMSRSDVGE 285

BLAST of Chy4G082580 vs. TAIR 10
Match: AT4G18910.1 (NOD26-like intrinsic protein 1;2 )

HSP 1 Score: 221.1 bits (562), Expect = 1.2e-57
Identity = 119/244 (48.77%), Postives = 161/244 (65.98%), Query Frame = 0

Query: 46  FSRKLVAEVIATYLLVFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAH 105
           F +KL+AEV+ TY L+F  C A A+N    + V+  G ++  GL V V++Y++GH+SGAH
Sbjct: 50  FLQKLMAEVLGTYFLIFAGCAAVAVNTQHDKAVTLPGIAIVWGLTVMVLVYSLGHISGAH 109

Query: 106 MNPAVTMAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRLLLHPIKHLGT--------TT 165
            NPAVT+AFA+   FP KQVP Y  +Q+ G+T AA TLRLL    + + +        T 
Sbjct: 110 FNPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDQDVCSGKHDVFVGTL 169

Query: 166 PSGSDLQALVMEIVVTFSMMFVTLAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSM 225
           PSGS+LQ+ V+E ++TF +MFV   VATD +A+GELAG+AVGS V +  I+AGPVSG SM
Sbjct: 170 PSGSNLQSFVIEFIITFYLMFVISGVATDNRAIGELAGLAVGSTVLLNVIIAGPVSGASM 229

Query: 226 NPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMRR 282
           NP R+LGPA+    Y+GLW+Y V P+VG   GAW Y  +R +DKP+  I+     LK  R
Sbjct: 230 NPGRSLGPAMVYSCYRGLWIYIVSPIVGAVSGAWVYNMVRYTDKPLREITKSGSFLKTVR 289

BLAST of Chy4G082580 vs. TAIR 10
Match: AT5G37820.1 (NOD26-like intrinsic protein 4;2 )

HSP 1 Score: 215.3 bits (547), Expect = 6.4e-56
Identity = 119/263 (45.25%), Postives = 166/263 (63.12%), Query Frame = 0

Query: 16  VDVNEFVSVENPDSKRSKFG---SFFKNPYPPGFSRKLVAEVIATYLLVFVTCGAAALNA 75
           ++  +   +E  + K S+ G   +   +P     ++KL+AE+I TY ++F  CG   +N 
Sbjct: 8   IEDEQISRIEKGNCKDSQGGMETAICSSPSIVCLTQKLIAEMIGTYFIIFSGCGVVVVNV 67

Query: 76  SDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHFPWKQVPLYGAAQ 135
                ++  G  V  GLIV VMIY+ GH+SGAH NPAVT+ FA  R FPW QVPLY  AQ
Sbjct: 68  LYGGTITFPGICVTWGLIVMVMIYSTGHISGAHFNPAVTVTFAVFRRFPWYQVPLYIGAQ 127

Query: 136 LSGATCAAFTLRLLLHPI-KHLGTTTPSGSDLQALVMEIVVTFSMMFVTLAVATDTKAVG 195
           L+G+  A+ TLRL+ +   K    TTP+ S  QALV EI+++F +MFV   VATD++A G
Sbjct: 128 LTGSLLASLTLRLMFNVTPKAFFGTTPTDSSGQALVAEIIISFLLMFVISGVATDSRATG 187

Query: 196 ELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAW 255
           ELAGIAVG  + +   +AGP+SG SMNP R+LGPA+    YKG+WVY VGP VG   G +
Sbjct: 188 ELAGIAVGMTIILNVFVAGPISGASMNPARSLGPAIVMGRYKGIWVYIVGPFVGIFAGGF 247

Query: 256 SYKFIRASDKPVHLISPHSFSLK 275
            Y F+R +DKP+  ++  +  L+
Sbjct: 248 VYNFMRFTDKPLRELTKSASFLR 270

BLAST of Chy4G082580 vs. TAIR 10
Match: AT5G37810.1 (NOD26-like intrinsic protein 4;1 )

HSP 1 Score: 212.6 bits (540), Expect = 4.1e-55
Identity = 115/230 (50.00%), Postives = 152/230 (66.09%), Query Frame = 0

Query: 47  SRKLVAEVIATYLLVFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHM 106
           ++KL+AE+I TY +VF  CG   +N      ++  G  V  GLIV VMIY+ GH+SGAH 
Sbjct: 42  TQKLIAEMIGTYFIVFSGCGVVVVNVLYGGTITFPGICVTWGLIVMVMIYSTGHISGAHF 101

Query: 107 NPAVTMAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRLL--LHPIKHLGTTTPSGSDLQ 166
           NPAVT+ FA  R FPW QVPLY  AQ +G+  A+ TLRL+  + P    G TTP+ S  +
Sbjct: 102 NPAVTVTFAIFRRFPWHQVPLYIGAQFAGSLLASLTLRLMFKVTPEAFFG-TTPADSPAR 161

Query: 167 ALVMEIVVTFSMMFVTLAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLG 226
           ALV EI+++F +MFV   VATD +AVGELAGIAVG  + +   +AGP+SG SMNP R+LG
Sbjct: 162 ALVAEIIISFLLMFVISGVATDNRAVGELAGIAVGMTIMVNVFVAGPISGASMNPARSLG 221

Query: 227 PALASDYYKGLWVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLK 275
           PAL    YK +WVY VGPV+G   G + Y  IR +DKP+  ++  +  L+
Sbjct: 222 PALVMGVYKHIWVYIVGPVLGVISGGFVYNLIRFTDKPLRELTKSASFLR 270

BLAST of Chy4G082580 vs. TAIR 10
Match: AT4G19030.1 (NOD26-like major intrinsic protein 1 )

HSP 1 Score: 211.1 bits (536), Expect = 1.2e-54
Identity = 116/242 (47.93%), Postives = 159/242 (65.70%), Query Frame = 0

Query: 46  FSRKLVAEVIATYLLVFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAH 105
           F +KL+AE + TY LVF  C +  +N  +   V+  G ++  GL + V+IY++GH+SGAH
Sbjct: 53  FLQKLIAEFLGTYFLVFTGCASVVVNMQNDNVVTLPGIAIVWGLTIMVLIYSLGHISGAH 112

Query: 106 MNPAVTMAFAATRHFPWKQVPLYGAAQLSGATCAAFTLRLLL---HPI---KH---LGTT 165
           +NPAVT+AFA+   FP KQVP Y  +Q+ G+T AA TLRLL    H +   KH   +G +
Sbjct: 113 INPAVTIAFASCGRFPLKQVPAYVISQVIGSTLAAATLRLLFGLDHDVCSGKHDVFIG-S 172

Query: 166 TPSGSDLQALVMEIVVTFSMMFVTLAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGS 225
           +P GSDLQA  ME +VTF +MF+   VATD +A+GELAG+A+GS V +  ++A PVS  S
Sbjct: 173 SPVGSDLQAFTMEFIVTFYLMFIISGVATDNRAIGELAGLAIGSTVLLNVLIAAPVSSAS 232

Query: 226 MNPVRTLGPALASDYYKGLWVYFVGPVVGTQLGAWSYKFIRASDKPVHLISPHSFSLKMR 279
           MNP R+LGPAL    YKG+W+Y V P +G   GAW Y  +R +DKP+  I+     LK  
Sbjct: 233 MNPGRSLGPALVYGCYKGIWIYLVAPTLGAIAGAWVYNTVRYTDKPLREITKSGSFLKTV 292

BLAST of Chy4G082580 vs. TAIR 10
Match: AT1G31885.1 (NOD26-like intrinsic protein 3;1 )

HSP 1 Score: 204.9 bits (520), Expect = 8.7e-53
Identity = 119/295 (40.34%), Postives = 179/295 (60.68%), Query Frame = 0

Query: 1   MSSIQNPVLSNQETVVDVNEFVSVENPDSKRSKFGSFFKNPYPPGFSRKLVAEVIATYLL 60
           M+ I +     Q  V+D+  + S++  DS+ S   +   +     F +KL+ E + T+ +
Sbjct: 1   MAEISDITTQTQTVVLDIENYQSID--DSRSSDLSAPLVS---VSFVQKLIGEFVGTFTM 60

Query: 61  VFVTCGAAALNASDARRVSQLGASVAGGLIVTVMIYAVGHVSGAHMNPAVTMAFAATRHF 120
           +F  C A  +N +  + V+  G ++  GL+VTVMIY++GHVSGAH NPAV++AFA+++ F
Sbjct: 61  IFAGCSAIVVNETYGKPVTLPGIALVWGLVVTVMIYSIGHVSGAHFNPAVSIAFASSKKF 120

Query: 121 PWKQVPLYGAAQLSGATCAAFTLRLLLH------PIK---HLGTTTPSGSDLQALVMEIV 180
           P+ QVP Y AAQL G+T AA  LRL+ H       +K   ++G T PS S+  + VME +
Sbjct: 121 PFNQVPGYIAAQLLGSTLAAAVLRLVFHLDDDVCSLKGDVYVG-TYPSNSNTTSFVMEFI 180

Query: 181 VTFSMMFVTLAVATDTKAVGELAGIAVGSAVCITSILAGPVSGGSMNPVRTLGPALASDY 240
            TF++MFV  AVATD +A G  AGIA+G+ + +  + +GP+SG SMNP R+LGPAL    
Sbjct: 181 ATFNLMFVISAVATDKRATGSFAGIAIGATIVLDILFSGPISGASMNPARSLGPALIWGC 240

Query: 241 YKGLWVYFVGPVVGTQLGAWSYKFIRASDKPV-HLISPHSFSLKMRRMSRSDVSE 286
           YK LW+Y V PV+G   GAW+Y  +R++ K    +I P+   +  R    +   E
Sbjct: 241 YKDLWLYIVSPVIGALSGAWTYGLLRSTKKSYSEIIRPNCNKVSSRDRQEASQDE 289

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q19KC11.2e-8660.97Aquaporin NIP2-1 OS=Zea mays OX=4577 GN=NIP2-1 PE=2 SV=2[more]
Q6Z2T31.7e-8560.84Aquaporin NIP2-1 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-1 PE=1 SV=1[more]
Q9ATN22.2e-8566.67Aquaporin NIP2-2 OS=Zea mays OX=4577 GN=NIP2-2 PE=2 SV=1[more]
Q67WJ84.1e-8460.58Aquaporin NIP2-2 OS=Oryza sativa subsp. japonica OX=39947 GN=NIP2-2 PE=1 SV=1[more]
Q9AT743.0e-8263.39Aquaporin NIP2-3 OS=Zea mays OX=4577 GN=NIP2-3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
G8XUV33.3e-15399.31Si transport-like protein 1 OS=Cucumis sativus OX=3659 GN=Csa_3G826640 PE=2 SV=1[more]
A0A1L7B7J03.3e-15399.31LSi1 OS=Cucumis sativus var. sativus OX=869827 GN=LSi1 PE=2 SV=1[more]
A0A5D3DQ375.0e-14997.57Aquaporin NIP2-1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35... [more]
A0A1S3B8075.0e-14997.57aquaporin NIP2-1-like OS=Cucumis melo OX=3656 GN=LOC103487002 PE=3 SV=1[more]
Q7X9T08.0e-13991.58Nod26-like protein OS=Cucurbita pepo OX=3663 GN=NIP1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
NP_001267699.13.52e-19899.31aquaporin NIP2-1-like [Cucumis sativus] >ACU29603.1 Si transport-like protein 1 ... [more]
XP_008443411.11.04e-19297.57PREDICTED: aquaporin NIP2-1-like [Cucumis melo] >KAA0053759.1 aquaporin NIP2-1-l... [more]
XP_038905688.17.16e-18894.74aquaporin NIP2-1-like [Benincasa hispida][more]
XP_023529347.12.79e-17991.58aquaporin NIP2-1-like [Cucurbita pepo subsp. pepo] >KAG7022218.1 Aquaporin NIP2-... [more]
XP_022934019.11.13e-17891.23aquaporin NIP2-1-like [Cucurbita moschata] >BAK09175.1 silicon transporter 1 [Cu... [more]
Match NameE-valueIdentityDescription
AT4G18910.11.2e-5748.77NOD26-like intrinsic protein 1;2 [more]
AT5G37820.16.4e-5645.25NOD26-like intrinsic protein 4;2 [more]
AT5G37810.14.1e-5550.00NOD26-like intrinsic protein 4;1 [more]
AT4G19030.11.2e-5447.93NOD26-like major intrinsic protein 1 [more]
AT1G31885.18.7e-5340.34NOD26-like intrinsic protein 3;1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 199..221
score: 34.94
coord: 236..256
score: 37.0
coord: 124..143
score: 36.12
coord: 168..186
score: 28.87
coord: 48..67
score: 38.08
coord: 87..111
score: 48.82
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 46..253
e-value: 6.3E-54
score: 183.1
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 48..256
e-value: 1.11028E-56
score: 180.142
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 10..263
e-value: 1.0E-68
score: 233.0
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 43..266
IPR034294Aquaporin transporterPANTHERPTHR45724AQUAPORIN NIP2-1coord: 19..278
NoneNo IPR availablePANTHERPTHR45724:SF30MAJOR INTRINSIC PROTEIN, AQUAPORIN-LIKE PROTEIN-RELATEDcoord: 19..278
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 105..113

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G082580.1Chy4G082580.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015267 channel activity