Chy4G080670 (gene) Cucumber (hystrix) v1

Overview
NameChy4G080670
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMyricetin O-methyltransferase-like
LocationchrH04: 20112264 .. 20114278 (-)
RNA-Seq ExpressionChy4G080670
SyntenyChy4G080670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGGAAACATTGAATTGGTTGAAGCTCAAGCTCATATTTGGAATCATGCGTTGAAATACATCAACTCCATGTCTTTAAAATGTGTAGTTGAATTAGGCATAGCAGACATTATCCACAACCATGGCCAACCCATGAGCCTTTCCCAATTACTTCAATCACTCCATATTCATCCCTCCAAAGCTCAATGCCTTAGCCGCCTTATGCGCCTCCTCGTTCATTCCGGCTTCTTTGCACAAACTCAACCTGACTTCTTCTCTCTCACTCCTCCCTCTCGTCTTCTCCTTCGTGAACCCAACAAAACAGCATTCGATACCTCGCCCTTCTTACTTCTAACTCTTAGCCCACTCATGATGTCTCCATGGCAAACCATGAGCAAATGGCTATGTAGTCAAGATGATGATCAACATCAAGACCATTATTCAACTGCTTTTGAAATGGCAAATGGGAAATCAATTTGGGATTATGTTAAAGAAGAAGAACAAAGTTGTGGTTTCGGGAAGCTATTCCATCAAACATTGGAGTGTGATTCTCGATTAATTGGGAAAGTGGTGAGTAGTGAATGTGGAGAGATGCTTGAAGGGTTGAGGTCATTGGTTGATGTTGGTGGAGGTGCTGGTGCTATGGCTAAGGCCATTGTGGAAGCATTCCCACATATCAACTGCTTTGTGCTTGATCTCCCTGAGGTTGTTGCTAACCAAAAGCCAAAACAACAAATACAAAACCTACACTTCATTGTAGGTGACATGTTTCAAAAAATTCCACCAGCAAATGCAGTCCTATTGAAGGTATATATGTCTGCTCCTTTCCTCCCTAGTATTTTCTAATATTATATATGTTTTATATTGACATTTTTTCACTTTACGTTAATAATACACGGATTACTAAACAACAACCATGATTTTATAATTTTAAATGATGTGAATCTCAATTTTTTTTGAAGCAATGAGAATTCCAATAAAAATATTTAACTGCATCTCAAATTACACCTATCTTTATGCATCTCACCAAAATTATGCACTCATATAATTTGTCAAATAGATAAATTGTTTTTACTTTTTTTAAATACCTTAATTCATTTGAATATATATGATGACCGTGAAATGTATAAAAATATACAGATACATTTTACAATACACCCTCAAGTATTGCTCAAATCTAATCTAACATACATAGTTAATAATAACAAGTATCTAAATTATATTTTGAAAAGCTTCTAATAGATAGTTAATCTGAGTAACTTTTTTAATTGATTTCTATTTAGTGTTTAATATATGTGTCATTAAATTTTATAGTCTCTCTAATGAGTCTATAAAATTTTCTTTTGTATATAAAAGTTCCTATGAATAGTCTTTGACTTAGTCAACATTTTCTAAATTTTTTCTAAATTTGCCAATTCTATTGTACACAAAATTGAAAGATTTAAATTTTGTGTCTATGATTCACATTAGTCTTAGAAAATATTAAACACATGGATGATATAATTTGAGCTTCAAAGATATATTTATTGGACTTCTTAAAGTTGATTCAATATGACAGTTATTAGAAACAAAAATGATATACCTATTAAATACATTATTTTGAAACGACTCATTAAACTTTATACAAATGCATAAAAAGTCATGCAAGGATTAAGATTGTTTAGAGAGAGACTAATTAATTAATATTCCTTTCCACTTTGGGGTGTGTTTGTTATTAGTCAATTCTACACGATTGGAATGATGAAGAAAGCATTAAGATATTGAAGAATTGTAAAGAAGCAATTCCAAGCAGAGAGGAGGGAGGGAAAGTGATAATAATAGAGATGGTGTTAGAAAAAGAGTTGGAAGAGATGAAGAAATCATCAGTTGAAACTGAAACTAAACTTTGCTGCGATGTGTTAATGATGACAATTTTCAGTGGAAGAGAAAGGAATGAGAAAGAATGGAATAATCTTTTCTTAGCTGCTGGCTTTGCTCACTACAACATAATTCCTATATTGGGTTTAAGGTCTCTCATTGTGGTCTACCCCTAA

mRNA sequence

ATGGAAGGAAACATTGAATTGGTTGAAGCTCAAGCTCATATTTGGAATCATGCGTTGAAATACATCAACTCCATGTCTTTAAAATGTGTAGTTGAATTAGGCATAGCAGACATTATCCACAACCATGGCCAACCCATGAGCCTTTCCCAATTACTTCAATCACTCCATATTCATCCCTCCAAAGCTCAATGCCTTAGCCGCCTTATGCGCCTCCTCGTTCATTCCGGCTTCTTTGCACAAACTCAACCTGACTTCTTCTCTCTCACTCCTCCCTCTCGTCTTCTCCTTCGTGAACCCAACAAAACAGCATTCGATACCTCGCCCTTCTTACTTCTAACTCTTAGCCCACTCATGATGTCTCCATGGCAAACCATGAGCAAATGGCTATGTAGTCAAGATGATGATCAACATCAAGACCATTATTCAACTGCTTTTGAAATGGCAAATGGGAAATCAATTTGGGATTATGTTAAAGAAGAAGAACAAAGTTGTGGTTTCGGGAAGCTATTCCATCAAACATTGGAGTGTGATTCTCGATTAATTGGGAAAGTGGTGAGTAGTGAATGTGGAGAGATGCTTGAAGGGTTGAGGTCATTGGTTGATGTTGGTGGAGGTGCTGGTGCTATGGCTAAGGCCATTGTGGAAGCATTCCCACATATCAACTGCTTTGTGCTTGATCTCCCTGAGGTTGTTGCTAACCAAAAGCCAAAACAACAAATACAAAACCTACACTTCATTGTAGGTGACATGTTTCAAAAAATTCCACCAGCAAATGCAGTCCTATTGAAGTCAATTCTACACGATTGGAATGATGAAGAAAGCATTAAGATATTGAAGAATTGTAAAGAAGCAATTCCAAGCAGAGAGGAGGGAGGGAAAGTGATAATAATAGAGATGGTGTTAGAAAAAGAGTTGGAAGAGATGAAGAAATCATCAGTTGAAACTGAAACTAAACTTTGCTGCGATGTGTTAATGATGACAATTTTCAGTGGAAGAGAAAGGAATGAGAAAGAATGGAATAATCTTTTCTTAGCTGCTGGCTTTGCTCACTACAACATAATTCCTATATTGGGTTTAAGGTCTCTCATTGTGGTCTACCCCTAA

Coding sequence (CDS)

ATGGAAGGAAACATTGAATTGGTTGAAGCTCAAGCTCATATTTGGAATCATGCGTTGAAATACATCAACTCCATGTCTTTAAAATGTGTAGTTGAATTAGGCATAGCAGACATTATCCACAACCATGGCCAACCCATGAGCCTTTCCCAATTACTTCAATCACTCCATATTCATCCCTCCAAAGCTCAATGCCTTAGCCGCCTTATGCGCCTCCTCGTTCATTCCGGCTTCTTTGCACAAACTCAACCTGACTTCTTCTCTCTCACTCCTCCCTCTCGTCTTCTCCTTCGTGAACCCAACAAAACAGCATTCGATACCTCGCCCTTCTTACTTCTAACTCTTAGCCCACTCATGATGTCTCCATGGCAAACCATGAGCAAATGGCTATGTAGTCAAGATGATGATCAACATCAAGACCATTATTCAACTGCTTTTGAAATGGCAAATGGGAAATCAATTTGGGATTATGTTAAAGAAGAAGAACAAAGTTGTGGTTTCGGGAAGCTATTCCATCAAACATTGGAGTGTGATTCTCGATTAATTGGGAAAGTGGTGAGTAGTGAATGTGGAGAGATGCTTGAAGGGTTGAGGTCATTGGTTGATGTTGGTGGAGGTGCTGGTGCTATGGCTAAGGCCATTGTGGAAGCATTCCCACATATCAACTGCTTTGTGCTTGATCTCCCTGAGGTTGTTGCTAACCAAAAGCCAAAACAACAAATACAAAACCTACACTTCATTGTAGGTGACATGTTTCAAAAAATTCCACCAGCAAATGCAGTCCTATTGAAGTCAATTCTACACGATTGGAATGATGAAGAAAGCATTAAGATATTGAAGAATTGTAAAGAAGCAATTCCAAGCAGAGAGGAGGGAGGGAAAGTGATAATAATAGAGATGGTGTTAGAAAAAGAGTTGGAAGAGATGAAGAAATCATCAGTTGAAACTGAAACTAAACTTTGCTGCGATGTGTTAATGATGACAATTTTCAGTGGAAGAGAAAGGAATGAGAAAGAATGGAATAATCTTTTCTTAGCTGCTGGCTTTGCTCACTACAACATAATTCCTATATTGGGTTTAAGGTCTCTCATTGTGGTCTACCCCTAA

Protein sequence

MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPSKAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMSPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRLIGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQIQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMVLEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPILGLRSLIVVYP*
Homology
BLAST of Chy4G080670 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 345.5 bits (885), Expect = 7.4e-94
Identity = 185/368 (50.27%), Postives = 238/368 (64.67%), Query Frame = 0

Query: 6   ELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPSKAQCL 65
           EL+ AQAH+WNH   +I SMSLKC ++LGI DIIHNHG+PM+L +L+  L +HP ++QC+
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHGKPMTLPELVAKLPVHPKRSQCV 70

Query: 66  SRLMRLLVHSGFFAQ------TQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMM 125
            RLMR+LVHSGF A        + + + LT  SRLLL +    +    P +L  L P++ 
Sbjct: 71  YRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMD---DSLSIRPLVLAMLDPILT 130

Query: 126 SPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSR 185
            PW  +S W   Q+DD       T F  A  +S WDY   E Q       F++ +  D+R
Sbjct: 131 KPWHYLSAWF--QNDD------PTPFHTAYERSFWDYAGHEPQ---LNNSFNEAMASDAR 190

Query: 186 LIGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQ 245
           L+  V+  E   +  GL SLVDVGGG G +AKAI  AFPH+NC VLDL  VVA     Q 
Sbjct: 191 LLTSVLLKEGQGVFAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAG---LQG 250

Query: 246 IQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEM 305
            +NL++  GDMF+ IPPA+A+LLK ILHDW++EE +KILK C+EAIPS+E GGKVIII+M
Sbjct: 251 SKNLNYFAGDMFEAIPPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDM 310

Query: 306 VLEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPILGL 365
           ++ K   + K     TET+L  D+ MM    GRER+E EW  LFL AGF+HY I PILGL
Sbjct: 311 IMMKNQGDYK----STETQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGL 357

Query: 366 RSLIVVYP 368
           RSLI VYP
Sbjct: 371 RSLIEVYP 357

BLAST of Chy4G080670 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 6.9e-92
Identity = 185/377 (49.07%), Postives = 242/377 (64.19%), Query Frame = 0

Query: 2   EGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPSK 61
           E + E++ AQAHIWN    +INSMSLKC ++LGI DII+NHG+PM++SQL  +L I+  K
Sbjct: 21  ENSTEILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHGKPMTISQLTLALPINRKK 80

Query: 62  AQCLSRLMRLLVHSGFFAQTQPDF----------FSLTPPSRLLLREPNKTAFDTSPFLL 121
           + C+ RLMR+L+HSGFFA  + +           + +T  S+LLL++        +PFLL
Sbjct: 81  SPCVYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKD---NPMSVTPFLL 140

Query: 122 LTLSPLMMSPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFH 181
             L P+M  PW  +S W  + D         T F+ ANG + WDY   E +     + F+
Sbjct: 141 AMLDPVMTKPWDFLSNWFQNGD--------PTPFDTANGMAFWDYGSHEPK---LARFFN 200

Query: 182 QTLECDSRLIGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVV 241
             +  D+RL+  VV  +C  + EG+ SLVDVGGG G +A +I  AFPHI C V DLP VV
Sbjct: 201 DAMASDARLVTSVVIEKCKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVV 260

Query: 242 ANQKPKQQIQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAI-PSREE 301
           A+    Q   NL+F+ GDMF  +P    VLLK ILHDWNDEES+KILK CKEAI  S ++
Sbjct: 261 AD---LQGGNNLNFVGGDMFVDVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKK 320

Query: 302 GGKVIIIEMVLEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAH 361
           GGKVIII+M +E E +E  +S    ET+L  D+LMM + +GRERNEKEW  LF  AGF++
Sbjct: 321 GGKVIIIDMKVENEKDEDDES---YETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSN 377

Query: 362 YNIIPILGLRSLIVVYP 368
           Y I PILGLRSLI VYP
Sbjct: 381 YKITPILGLRSLIEVYP 377

BLAST of Chy4G080670 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 5.1e-87
Identity = 182/370 (49.19%), Postives = 239/370 (64.59%), Query Frame = 0

Query: 6   ELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQP--MSLSQLLQSLHIHPSKAQ 65
           EL+ AQAHI+NH   +I+SM+LKC VEL I +I+HNH QP  ++LS+L+Q+L I  +K+ 
Sbjct: 23  ELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNH-QPKAVTLSELVQALQIPQAKSA 82

Query: 66  CLSRLMRLLVHSGFFAQTQ------PDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPL 125
           CL RL+R+LVHSGFFA T+       + +  T  S+LLL+         SP LL  ++P 
Sbjct: 83  CLYRLLRILVHSGFFAITKIQSEGDEEGYLPTLSSKLLLK---NHPMSMSPCLLGLVNPT 142

Query: 126 MMSPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECD 185
           M++P    S W    DD        T FE  +G S+W Y  E        ++F++ + C+
Sbjct: 143 MVAPMHFFSDWFKRSDD-------MTPFEATHGASLWKYFGETPH---MAEIFNEAMGCE 202

Query: 186 SRLIGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPK 245
           +RL   VV  EC   LEG+ SLVDVGGG G + +AI EAFP++ C VLDLP+VV N K  
Sbjct: 203 TRLAMSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGS 262

Query: 246 QQIQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIII 305
               NL F+ GDMFQ IPPA+ V LK ILHDWNDEE IKILK CKEAIPS+EEGGK+III
Sbjct: 263 ---NNLEFVSGDMFQFIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLIII 322

Query: 306 EMVLEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPIL 365
           +MV+    +  K S   TET+L  D+ +M + +G ER E EW  LF+AAGF  Y I P+L
Sbjct: 323 DMVVN---DHNKGSYESTETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVL 372

Query: 366 GLRSLIVVYP 368
           GL+S+I V+P
Sbjct: 383 GLKSIIEVFP 372

BLAST of Chy4G080670 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 3.1e-84
Identity = 172/377 (45.62%), Postives = 234/377 (62.07%), Query Frame = 0

Query: 6   ELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPSKAQCL 65
           EL+EAQAH+WNH   YINSMSLKC ++LGI D IH HG P++LSQL  +L+I+ +K+  L
Sbjct: 12  ELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHGNPITLSQLADALNINKAKSHGL 71

Query: 66  SRLMRLLVHSGFF-------------AQTQPDFFSLTPPSRLLLR-EPNKTAFDTSPFLL 125
            RLMR+LVHSGFF              + + D +SLTP SRLLLR EP       +PF L
Sbjct: 72  FRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEP----LSVAPFAL 131

Query: 126 LTLSPLMMSPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFH 185
               P+    W  +S+W         ++    AF+   G +  +Y   +++      LF+
Sbjct: 132 AMSDPVYTETWHHLSEWF--------RNDAVAAFDTKYGMTFPEYAVADDR---LNVLFN 191

Query: 186 QTLECDSRLIGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVV 245
           + + CD+  +  ++++EC E+ +GL S+VDVGGG GA AK I  AFP + C VLDLP VV
Sbjct: 192 EAMACDAGFVNSILTTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVV 251

Query: 246 ANQKPKQQIQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIP-SREE 305
              K     +NL F+ GDMF  IP A+A+ +K ILHDWNDEE +KILK CKEAI  S   
Sbjct: 252 GGLKGS---ENLSFVSGDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNS 311

Query: 306 GGKVIIIEMVLEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAH 365
             K+I++E+V+E E    K++   TETKL  D+ M+ I +G+ER+EKEW  LF  AGF +
Sbjct: 312 CRKIILVEIVMEDE----KETHEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTN 366

Query: 366 YNIIPILGLRSLIVVYP 368
           Y I  +LGLRS+I V+P
Sbjct: 372 YKITRVLGLRSVIEVFP 366

BLAST of Chy4G080670 vs. ExPASy Swiss-Prot
Match: Q93WU2 (Eugenol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=EOMT1 PE=1 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 2.6e-83
Identity = 171/369 (46.34%), Postives = 230/369 (62.33%), Query Frame = 0

Query: 6   ELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPSKAQCL 65
           +L++AQ H+WNH   + NSMSLKC ++LGI DI+H HG+PM+LSQLLQS+ I+  K QC 
Sbjct: 14  QLLQAQVHVWNHMYAFANSMSLKCAIQLGIPDILHKHGRPMTLSQLLQSIPINKEKTQCF 73

Query: 66  SRLMRLLVHSGFF-----AQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMS 125
            RLMR LV+S FF     +  Q   + LTP S LLL+E   T    +P + + L P   +
Sbjct: 74  QRLMRALVNSNFFIEENNSNNQEVCYWLTPASCLLLKEAPLT---VTPLVQVVLDPTFTN 133

Query: 126 PWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRL 185
           PW  MS+W        H+ H +T FE ANG + W+ +  E      G+ F + + CDSRL
Sbjct: 134 PWHHMSEWF------THEKH-ATQFEAANGCTFWEKLANEPSK---GRFFDEAMSCDSRL 193

Query: 186 IGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQI 245
           I  V + +   ++EG+R+LVDVGGG G MAKAIVEA P I C V+DLP VVA     +  
Sbjct: 194 IAHVFTKDYKHVIEGIRTLVDVGGGNGTMAKAIVEAMPTIKCTVIDLPHVVAG---LEST 253

Query: 246 QNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMV 305
            NL++I GDMFQ IP A+A+LLKSI+HDW+D E +KILK CK+A+     GGKVIII++V
Sbjct: 254 DNLNYIGGDMFQSIPSADAILLKSIIHDWDDVEGLKILKKCKDAVVM---GGKVIIIDVV 313

Query: 306 --LEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPILG 365
             +  +++E+       E +L  D+ MM  F+ +ER   EW  L   AGF  Y + P  G
Sbjct: 314 VGVNHDIDEV------LEDQLHFDMAMMCYFNAKERTMSEWEKLIYDAGFKSYKLTPAFG 357

Query: 366 LRSLIVVYP 368
           +RSLI  YP
Sbjct: 374 VRSLIEAYP 357

BLAST of Chy4G080670 vs. ExPASy TrEMBL
Match: A0A5D3CHG4 (Myricetin O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00430 PE=3 SV=1)

HSP 1 Score: 594.3 bits (1531), Expect = 3.4e-166
Identity = 293/316 (92.72%), Postives = 302/316 (95.57%), Query Frame = 0

Query: 1   MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPS 60
           ME NIELVEAQAHIWNH  KYINSMSLKCVVELGI DIIHNHGQPMSLSQLL+SLHIHPS
Sbjct: 1   MEENIELVEAQAHIWNHTFKYINSMSLKCVVELGIPDIIHNHGQPMSLSQLLESLHIHPS 60

Query: 61  KAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMS 120
           KAQCLSRLMRLLVHSGFFAQ QPDFFSLTPPSRLLLRE +KTAFDT+PFLLL LSPLMMS
Sbjct: 61  KAQCLSRLMRLLVHSGFFAQPQPDFFSLTPPSRLLLRENHKTAFDTTPFLLLILSPLMMS 120

Query: 121 PWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRL 180
           PWQTMSKWLCSQDDDQ QDHYSTAFE+ANGK IWDYVKEEE+SCGFGKLFHQTLECDSRL
Sbjct: 121 PWQTMSKWLCSQDDDQDQDHYSTAFELANGKPIWDYVKEEEESCGFGKLFHQTLECDSRL 180

Query: 181 IGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQI 240
           IGKVVSSECGEM EGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLP+VVANQKPKQ I
Sbjct: 181 IGKVVSSECGEMFEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPQVVANQKPKQHI 240

Query: 241 QNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMV 300
           QNLHFI GDMFQKIPPANAVLLKSILHDWNDEESIKILK CKE+IPSR EGGKVIIIEMV
Sbjct: 241 QNLHFIEGDMFQKIPPANAVLLKSILHDWNDEESIKILKKCKESIPSRGEGGKVIIIEMV 300

Query: 301 LEKELEEMKKSSVETE 317
           LEKELE+MKKSSVET+
Sbjct: 301 LEKELEQMKKSSVETQ 316

BLAST of Chy4G080670 vs. ExPASy TrEMBL
Match: A0A6J1F479 (probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111442010 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 5.8e-142
Identity = 260/366 (71.04%), Postives = 302/366 (82.51%), Query Frame = 0

Query: 1   MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPS 60
           M  N EL+EA+AH+WNH   YINSMSL+CVV+LGI DIIH+HG+PMSLS L+++LHI PS
Sbjct: 1   MGENRELLEAEAHVWNHTFNYINSMSLRCVVQLGIPDIIHSHGRPMSLSSLVEALHIQPS 60

Query: 61  KAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMS 120
           KAQCL RLMRLLVHSGFFA+T   FFSLT PSRLLLR+ N  AFDT+P LLL  SP+ M+
Sbjct: 61  KAQCLGRLMRLLVHSGFFAETHSGFFSLTTPSRLLLRQ-NHNAFDTTPLLLLLTSPVFMA 120

Query: 121 PWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRL 180
           PW+TMS WLCS D    +DH STAFEMANG +IWDY+   +Q  GFG LFHQTL  DS+L
Sbjct: 121 PWETMSNWLCSDD----KDHNSTAFEMANGNTIWDYI--AQQPSGFGNLFHQTLIHDSQL 180

Query: 181 IGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQI 240
           IGKVV+SEC  M EGL SLVDVGGGAGAMAKAIVEAFPHI+C V DLP+VVA+Q+P+Q  
Sbjct: 181 IGKVVTSECRGMFEGLTSLVDVGGGAGAMAKAIVEAFPHISCIVFDLPQVVASQEPQQAK 240

Query: 241 QNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMV 300
           +NL+FI GDMF+KIP ANA+LLK +LHDW+DEESI+ILKNCK AIPSR EGGKVIIIEMV
Sbjct: 241 KNLNFIGGDMFEKIPQANALLLKLVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEMV 300

Query: 301 LEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPILGLR 360
            E   E+  K SVET+  LCCDV MMT F+GRERNE+EW +LFLAAGF+ YNIIP+LGLR
Sbjct: 301 FE---EQRDKESVETQ--LCCDVYMMTTFTGRERNEREWKSLFLAAGFSDYNIIPVLGLR 354

Query: 361 SLIVVY 367
           S+I VY
Sbjct: 361 SIIEVY 354

BLAST of Chy4G080670 vs. ExPASy TrEMBL
Match: A0A6J1J5U8 (probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111481541 PE=3 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 7.5e-142
Identity = 260/366 (71.04%), Postives = 302/366 (82.51%), Query Frame = 0

Query: 1   MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPS 60
           M  N EL+EAQAH+WNH   YINSMSL+C+V+LGI DIIH+HG+PMSLS L+++LHI PS
Sbjct: 1   MGENRELLEAQAHVWNHTFNYINSMSLRCIVQLGIPDIIHSHGRPMSLSSLVEALHIQPS 60

Query: 61  KAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMS 120
           KAQCL RLMRLLVHSGFFA+T   FFSLT PSRLLLR+ N  AFDT+PFLLL LSP+ M+
Sbjct: 61  KAQCLGRLMRLLVHSGFFAETHSGFFSLTTPSRLLLRQ-NHNAFDTTPFLLLLLSPVFMA 120

Query: 121 PWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRL 180
           PW+TM+ WLCS D    QDH STAFEMANG +IWDY+   +Q  GFG LFHQ L  DS+L
Sbjct: 121 PWETMNDWLCSDD----QDHNSTAFEMANGNTIWDYI--AQQPSGFGNLFHQALIHDSQL 180

Query: 181 IGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQI 240
           IGKVV+SEC  M EGL SLVDVGGG GAMAKAIVEAFPHI+C V DLP+VVANQ+P+Q  
Sbjct: 181 IGKVVTSECRGMFEGLTSLVDVGGGGGAMAKAIVEAFPHISCIVFDLPQVVANQEPQQAK 240

Query: 241 QNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMV 300
           +NL+FI GDMF+KIP ANA+LLK +LHDW+DEESI+ILKNCK AIPSR EGGKVIIIE+V
Sbjct: 241 KNLNFIGGDMFEKIPQANALLLKFVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEIV 300

Query: 301 LEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPILGLR 360
           LE   E+  K SVET+  LC DV MMT F+GRERNE+EW +LFLAAGF+ YNIIP+LGLR
Sbjct: 301 LE---EQRDKESVETQ--LCLDVYMMTTFTGRERNEREWKSLFLAAGFSDYNIIPVLGLR 354

Query: 361 SLIVVY 367
           S+I VY
Sbjct: 361 SIIEVY 354

BLAST of Chy4G080670 vs. ExPASy TrEMBL
Match: A0A1S3CNB0 (myricetin O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103502839 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.4e-140
Identity = 252/271 (92.99%), Postives = 261/271 (96.31%), Query Frame = 0

Query: 46  MSLSQLLQSLHIHPSKAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFD 105
           MSLSQLL+SLHIHPSKAQCLSRLMRLLVHSGFFAQ QPDFFSLTPPSRLLLRE +KTAFD
Sbjct: 1   MSLSQLLESLHIHPSKAQCLSRLMRLLVHSGFFAQPQPDFFSLTPPSRLLLRENHKTAFD 60

Query: 106 TSPFLLLTLSPLMMSPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCG 165
           T+PFLLL LSPLMMSPWQTMSKWLCSQDDDQ QDHYSTAFE+ANGK IWDYVKEEE+SCG
Sbjct: 61  TTPFLLLILSPLMMSPWQTMSKWLCSQDDDQDQDHYSTAFELANGKPIWDYVKEEEESCG 120

Query: 166 FGKLFHQTLECDSRLIGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVL 225
           FGKLFHQTLECDSRLIGKVVSSECGEM EGLRSLVDVGGGAGAMAKAIVEAFPHINCFVL
Sbjct: 121 FGKLFHQTLECDSRLIGKVVSSECGEMFEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVL 180

Query: 226 DLPEVVANQKPKQQIQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAI 285
           DLP+VVANQKPKQ IQNLHFI GDMFQKIPPANAVLLKSILHDWNDEESIKILK CKE+I
Sbjct: 181 DLPQVVANQKPKQHIQNLHFIEGDMFQKIPPANAVLLKSILHDWNDEESIKILKKCKESI 240

Query: 286 PSREEGGKVIIIEMVLEKELEEMKKSSVETE 317
           PSR EGGKVIIIEMVLEKELE+MKKSSVET+
Sbjct: 241 PSRGEGGKVIIIEMVLEKELEQMKKSSVETQ 271

BLAST of Chy4G080670 vs. ExPASy TrEMBL
Match: A0A6J1E096 (probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111025195 PE=3 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 3.1e-127
Identity = 245/372 (65.86%), Postives = 289/372 (77.69%), Query Frame = 0

Query: 1   MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPS 60
           M  + EL+ AQAH+WNH  KYINSMSLKCVVELGI DIIH+HGQPMSLS L+ +LHI PS
Sbjct: 1   MGESTELLRAQAHVWNHTFKYINSMSLKCVVELGIPDIIHSHGQPMSLSNLVDALHIQPS 60

Query: 61  KAQCLSRLMRLLVHSGFFAQTQPD----FFSLTPPSRLLLREPNKTAFDTSPFLLLTLSP 120
           KAQCL+RLMRLLVH+GFF QT  D     ++LTP SRLLL     TAF+  PF+ L L+ 
Sbjct: 61  KAQCLNRLMRLLVHAGFFIQTHGDNEQEEYALTPSSRLLL--CRNTAFENIPFVFLILNR 120

Query: 121 LMMSPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLEC 180
            MM+P +TMS WLCS D     +H ++AFEMANGKSIWDYV +E     FGKLFHQT+  
Sbjct: 121 AMMAPLETMSSWLCSVD-----EHSNSAFEMANGKSIWDYVSQEHD---FGKLFHQTMVH 180

Query: 181 DSRLIGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKP 240
           DS+LIGKVV+ EC E+ EGL+SLVDVGG  G MAKAI EAFPHI C V DLP+V AN   
Sbjct: 181 DSQLIGKVVTRECREVFEGLKSLVDVGGSTGFMAKAIAEAFPHITCTVFDLPQVAANS-- 240

Query: 241 KQQIQNLHFIVGDMFQ-KIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVI 300
           +Q  +NL FI GDMF+ KIPPANAVLLK ILHDWNDEESI+ILKNCK+AIPSR E GKVI
Sbjct: 241 QQTTKNLSFIGGDMFEAKIPPANAVLLKWILHDWNDEESIRILKNCKDAIPSRAEXGKVI 300

Query: 301 IIEMVLEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIP 360
           +I+MV+E ++E+  K SVE +  LCCD+L+MT ++GRERNE+EW NLFL AGF+ Y II 
Sbjct: 301 VIDMVVENQIED--KESVEAQ--LCCDLLVMTSYNGRERNEREWKNLFLTAGFSDYKIIS 356

Query: 361 ILGLRSLIVVYP 368
           +LGLRSLI VYP
Sbjct: 361 MLGLRSLIEVYP 356

BLAST of Chy4G080670 vs. NCBI nr
Match: KAA0037695.1 (myricetin O-methyltransferase-like [Cucumis melo var. makuwa] >TYK10678.1 myricetin O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 595 bits (1534), Expect = 6.16e-213
Identity = 293/316 (92.72%), Postives = 302/316 (95.57%), Query Frame = 0

Query: 1   MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPS 60
           ME NIELVEAQAHIWNH  KYINSMSLKCVVELGI DIIHNHGQPMSLSQLL+SLHIHPS
Sbjct: 1   MEENIELVEAQAHIWNHTFKYINSMSLKCVVELGIPDIIHNHGQPMSLSQLLESLHIHPS 60

Query: 61  KAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMS 120
           KAQCLSRLMRLLVHSGFFAQ QPDFFSLTPPSRLLLRE +KTAFDT+PFLLL LSPLMMS
Sbjct: 61  KAQCLSRLMRLLVHSGFFAQPQPDFFSLTPPSRLLLRENHKTAFDTTPFLLLILSPLMMS 120

Query: 121 PWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRL 180
           PWQTMSKWLCSQDDDQ QDHYSTAFE+ANGK IWDYVKEEE+SCGFGKLFHQTLECDSRL
Sbjct: 121 PWQTMSKWLCSQDDDQDQDHYSTAFELANGKPIWDYVKEEEESCGFGKLFHQTLECDSRL 180

Query: 181 IGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQI 240
           IGKVVSSECGEM EGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLP+VVANQKPKQ I
Sbjct: 181 IGKVVSSECGEMFEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPQVVANQKPKQHI 240

Query: 241 QNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMV 300
           QNLHFI GDMFQKIPPANAVLLKSILHDWNDEESIKILK CKE+IPSR EGGKVIIIEMV
Sbjct: 241 QNLHFIEGDMFQKIPPANAVLLKSILHDWNDEESIKILKKCKESIPSRGEGGKVIIIEMV 300

Query: 301 LEKELEEMKKSSVETE 316
           LEKELE+MKKSSVET+
Sbjct: 301 LEKELEQMKKSSVETQ 316

BLAST of Chy4G080670 vs. NCBI nr
Match: XP_038905391.1 (trans-resveratrol di-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 560 bits (1442), Expect = 2.75e-198
Identity = 280/367 (76.29%), Postives = 313/367 (85.29%), Query Frame = 0

Query: 1   MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPS 60
           M  N EL++AQAHIWNH  K INSMSLKCVVELGI DIIHNHGQPMSLSQL+++LHIHPS
Sbjct: 1   MGENKELLQAQAHIWNHTFKCINSMSLKCVVELGIPDIIHNHGQPMSLSQLVEALHIHPS 60

Query: 61  KAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMS 120
           KAQCL RLMRLL+HSGFFAQTQ  FFSLTP SRLLL + +KT FDT   LLL LSPLMM+
Sbjct: 61  KAQCLGRLMRLLLHSGFFAQTQSGFFSLTPSSRLLLSQNHKTIFDTKTLLLLFLSPLMMA 120

Query: 121 PWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRL 180
           PWQTMS WLC+   D     YSTAF+MANGKSIWDYV +EE+S GFGKLFHQTL CDS+L
Sbjct: 121 PWQTMSNWLCASTQD-----YSTAFQMANGKSIWDYVIQEEESYGFGKLFHQTLVCDSQL 180

Query: 181 IGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQI 240
           IGKVV+SECGEM EGL SLVDVGGGAG MAK I+EAFPH+ C VLDLP+VVANQKP+Q I
Sbjct: 181 IGKVVTSECGEMFEGLSSLVDVGGGAGTMAKVILEAFPHLTCIVLDLPQVVANQKPQQPI 240

Query: 241 QNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMV 300
           +NL+FI GDMF+KIPPANAVLLKSILH WNDEESI+ILKNCK+AIPSR EGG+VIIIEMV
Sbjct: 241 KNLNFIEGDMFEKIPPANAVLLKSILHSWNDEESIQILKNCKDAIPSRAEGGRVIIIEMV 300

Query: 301 LEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPILGLR 360
           L+++  +M+      ET+LC DVLMMT FSGRERNE+EW  LFLAAGF+ YNIIPILGLR
Sbjct: 301 LDQKERDMES----IETQLCFDVLMMTTFSGRERNEREWKTLFLAAGFSDYNIIPILGLR 358

Query: 361 SLIVVYP 367
           SLI VYP
Sbjct: 361 SLIEVYP 358

BLAST of Chy4G080670 vs. NCBI nr
Match: XP_023528438.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 519 bits (1336), Expect = 3.23e-182
Identity = 262/366 (71.58%), Postives = 303/366 (82.79%), Query Frame = 0

Query: 1   MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPS 60
           M  N EL+EAQAH+WNH  KYINSMSL+C+V+LGI DIIH+HGQPMSLS L++ LHI PS
Sbjct: 1   MGENRELMEAQAHVWNHTFKYINSMSLRCIVQLGIPDIIHSHGQPMSLSSLVKELHIQPS 60

Query: 61  KAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMS 120
           KAQCL RLMRLLVHSGFFA+T   FFSLT PSRLLLR+ N  AFDT+P LLL +SP+ M+
Sbjct: 61  KAQCLGRLMRLLVHSGFFAETHSGFFSLTTPSRLLLRQ-NHNAFDTTPLLLLLISPVFMA 120

Query: 121 PWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRL 180
           PW+TMS WLCS D    +DH STAFEMANG +IWDY+ +  Q  GFG LFHQTL  DS+L
Sbjct: 121 PWETMSNWLCSDD----KDHNSTAFEMANGNTIWDYIAQ--QPSGFGNLFHQTLIHDSQL 180

Query: 181 IGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQI 240
           IGKVV+SEC  M EG+ SLVDVGGG GAMAKAIVEAFPHI+C V DLP+VVANQ+P+Q  
Sbjct: 181 IGKVVTSECRGMFEGVTSLVDVGGGGGAMAKAIVEAFPHISCIVFDLPQVVANQEPQQAK 240

Query: 241 QNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMV 300
           +NL+FI GDMF+KIP ANA+LLK +LHDW+DEESI+ILKNCK AIPSR EGGKVIIIEMV
Sbjct: 241 KNLNFIGGDMFEKIPQANALLLKFVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEMV 300

Query: 301 LEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPILGLR 360
           LE   E+  K SVET+  LCCDV MMT F+GRERNE+EW +LFLAAGF+ YNIIP+LGLR
Sbjct: 301 LE---EQRDKESVETQ--LCCDVYMMTTFTGRERNEREWKSLFLAAGFSDYNIIPVLGLR 354

Query: 361 SLIVVY 366
           S+I VY
Sbjct: 361 SIIEVY 354

BLAST of Chy4G080670 vs. NCBI nr
Match: XP_022935024.1 (probable O-methyltransferase 3 [Cucurbita moschata])

HSP 1 Score: 513 bits (1322), Expect = 4.35e-180
Identity = 260/366 (71.04%), Postives = 302/366 (82.51%), Query Frame = 0

Query: 1   MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPS 60
           M  N EL+EA+AH+WNH   YINSMSL+CVV+LGI DIIH+HG+PMSLS L+++LHI PS
Sbjct: 1   MGENRELLEAEAHVWNHTFNYINSMSLRCVVQLGIPDIIHSHGRPMSLSSLVEALHIQPS 60

Query: 61  KAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMS 120
           KAQCL RLMRLLVHSGFFA+T   FFSLT PSRLLLR+ N  AFDT+P LLL  SP+ M+
Sbjct: 61  KAQCLGRLMRLLVHSGFFAETHSGFFSLTTPSRLLLRQ-NHNAFDTTPLLLLLTSPVFMA 120

Query: 121 PWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRL 180
           PW+TMS WLCS D    +DH STAFEMANG +IWDY+ +  Q  GFG LFHQTL  DS+L
Sbjct: 121 PWETMSNWLCSDD----KDHNSTAFEMANGNTIWDYIAQ--QPSGFGNLFHQTLIHDSQL 180

Query: 181 IGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQI 240
           IGKVV+SEC  M EGL SLVDVGGGAGAMAKAIVEAFPHI+C V DLP+VVA+Q+P+Q  
Sbjct: 181 IGKVVTSECRGMFEGLTSLVDVGGGAGAMAKAIVEAFPHISCIVFDLPQVVASQEPQQAK 240

Query: 241 QNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMV 300
           +NL+FI GDMF+KIP ANA+LLK +LHDW+DEESI+ILKNCK AIPSR EGGKVIIIEMV
Sbjct: 241 KNLNFIGGDMFEKIPQANALLLKLVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEMV 300

Query: 301 LEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPILGLR 360
            E   E+  K SVET+  LCCDV MMT F+GRERNE+EW +LFLAAGF+ YNIIP+LGLR
Sbjct: 301 FE---EQRDKESVETQ--LCCDVYMMTTFTGRERNEREWKSLFLAAGFSDYNIIPVLGLR 354

Query: 361 SLIVVY 366
           S+I VY
Sbjct: 361 SIIEVY 354

BLAST of Chy4G080670 vs. NCBI nr
Match: XP_023520752.1 (probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513 bits (1322), Expect = 4.35e-180
Identity = 260/366 (71.04%), Postives = 302/366 (82.51%), Query Frame = 0

Query: 1   MEGNIELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNHGQPMSLSQLLQSLHIHPS 60
           M  N EL+EAQAH+WNH  KYINSMSL+C+V+LGI DIIH+HGQPMSLS L++ LHI PS
Sbjct: 1   MGENRELMEAQAHVWNHTFKYINSMSLRCIVQLGIPDIIHSHGQPMSLSSLVKELHIQPS 60

Query: 61  KAQCLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMS 120
           KAQCL RLMRLLVHSGFFA+T   FFSLT PSRLLLR+ N  AFDT+P LLL +SP+ M+
Sbjct: 61  KAQCLGRLMRLLVHSGFFAETHSGFFSLTTPSRLLLRQ-NHNAFDTTPLLLLLISPVFMA 120

Query: 121 PWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRL 180
           PW+TMS WLCS D    +DH STAFEMANG +IWDY+ +  Q  GFG LFHQTL  DS+L
Sbjct: 121 PWETMSNWLCSDD----KDHNSTAFEMANGNTIWDYIAQ--QPSGFGNLFHQTLIHDSQL 180

Query: 181 IGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQI 240
           IGKVV+SEC  M EG+ SLVDVGGG GAMAKAIVEAFPHI+C V DLP+VVANQ+P+Q  
Sbjct: 181 IGKVVTSECRGMFEGVTSLVDVGGGGGAMAKAIVEAFPHISCIVFDLPQVVANQEPQQAK 240

Query: 241 QNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMV 300
           +NL+FI GDMF+KIP ANA+LLK +LHDW+DEESI+ILKNCK AIPSR EGGKVIIIE+V
Sbjct: 241 KNLNFIGGDMFEKIPQANALLLKFVLHDWSDEESIQILKNCKAAIPSRAEGGKVIIIEIV 300

Query: 301 LEKELEEMKKSSVETETKLCCDVLMMTIFSGRERNEKEWNNLFLAAGFAHYNIIPILGLR 360
           LE   E+  K SVET+  LC DV MMT F+GRERNE+EW +LFLAAGF+ YNIIP+LGLR
Sbjct: 301 LE---EQRDKESVETQ--LCLDVYMMTTFTGRERNEREWKSLFLAAGFSDYNIIPVLGLR 354

Query: 361 SLIVVY 366
           S+I VY
Sbjct: 361 SIIEVY 354

BLAST of Chy4G080670 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 190.7 bits (483), Expect = 2.1e-48
Identity = 121/374 (32.35%), Postives = 197/374 (52.67%), Query Frame = 0

Query: 6   ELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNH--GQPMSLSQLLQSLHIHPSKAQ 65
           E  +A   IW +   + +  + KC ++L I + I NH   QP++L++L  ++   PS   
Sbjct: 25  EEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPSH-- 84

Query: 66  CLSRLMRLLVHSGFFAQTQ-----PDFFSLTPPSRLLL--REPNKTAFDTSPFLLLTLSP 125
            L R+MR LVH G F +          +  TP SR L+  R   K+    +PF+L   +P
Sbjct: 85  -LRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKS---LAPFVLFETTP 144

Query: 126 LMMSPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLEC 185
            M++PW  +S  + S  +          F+  +GK +W + ++         + ++ + C
Sbjct: 145 EMLAPWLRLSSVVSSPVNGS----TPPPFDAVHGKDVWSFAQDNP---FLSDMINEAMAC 204

Query: 186 DSRLIGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKP 245
           D+R +   V+  C  + +G+ ++VDVGGG G     +V+ FP I  F  DLP V+   + 
Sbjct: 205 DARRVVPRVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEV 264

Query: 246 KQQIQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVII 305
              ++N   + GDMF  IP  +A+ +K +LHDW D++ IKILKNCKEA+P     GKV+I
Sbjct: 265 LDGVEN---VEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNI--GKVLI 324

Query: 306 IEMVLEKELEEM---KKSSVETETKLCCDVLMMTIFS-GRERNEKEWNNLFLAAGFAHYN 365
           +E V+ +  + M   ++       +L  D++MM   S G+ER  KEW+ +   AGFA Y 
Sbjct: 325 VESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYE 380

Query: 366 IIPILGLRSLIVVY 367
           +  I  ++SLI+ Y
Sbjct: 385 VRDIDDVQSLIIAY 380

BLAST of Chy4G080670 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 162.5 bits (410), Expect = 6.3e-40
Identity = 112/367 (30.52%), Postives = 178/367 (48.50%), Query Frame = 0

Query: 6   ELVEAQAHIWNHALKYINSMSLKCVVELGIADIIHNH--GQPMSLSQLLQSLHIHPSKAQ 65
           E  +A   IW +   + +  + KC ++L I + I NH   QP++LS+L  ++   PS   
Sbjct: 11  EEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSH-- 70

Query: 66  CLSRLMRLLVHSGFFAQTQPDFFSLTPPSRLLLREPNKTAFDTSPFLLLTLSPLMMSPWQ 125
            L R+MR LVH G F +                  P K    T  +    LS  MM    
Sbjct: 71  -LRRIMRFLVHQGLFKEV-----------------PTKDGLATG-YTNTPLSRRMM---- 130

Query: 126 TMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGKLFHQTLECDSRLIGK 185
                                    +GK +W + ++   +    +L ++ + CD+R +  
Sbjct: 131 ---------------------ITKLHGKDLWAFAQD---NLCHSQLINEAMACDARRVVP 190

Query: 186 VVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLPEVVANQKPKQQIQNL 245
            V+  C  + +G+ ++VDVGGG G     +V+ FP I  F  DLP V+   +  Q +  +
Sbjct: 191 RVAGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVI---EVAQVLDGV 250

Query: 246 HFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSREEGGKVIIIEMVLEK 305
             + GDMF  IP ++AV++K +LHDW D++ IKILKNCKEA+      GKV+I+E V+ +
Sbjct: 251 ENVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAV--LPNIGKVLIVECVIGE 310

Query: 306 ELEEM---KKSSVETETKLCCDVLMMTIFS-GRERNEKEWNNLFLAAGFAHYNIIPILGL 365
           +   M   ++       +L  D++MM   S G+ER  KEW+ +   AGFA Y +     +
Sbjct: 311 KKNTMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDV 323

Query: 366 RSLIVVY 367
           +SLI+ Y
Sbjct: 371 QSLIIAY 323

BLAST of Chy4G080670 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 140.6 bits (353), Expect = 2.6e-33
Identity = 114/367 (31.06%), Postives = 181/367 (49.32%), Query Frame = 0

Query: 6   ELVEAQAHIWNHALKYINSMSLKCVVELGIADII--HNHGQPMSLSQLLQSLHIHPSKAQ 65
           ++V  QA    +A+ +   M LK  +ELG+ D I   ++G  +S S++  SL   P+  +
Sbjct: 27  KMVSLQAESIVNAVAF--PMVLKAALELGVIDTIAAASNGTWLSPSEIAVSLPNKPTNPE 86

Query: 66  C---LSRLMRLLVHSGFFAQTQPD------------FFSLTPPSRLLLREPNKTAFDTSP 125
               L R++RLLV          +             ++  P  +  L++ + +    S 
Sbjct: 87  APVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDSDGSG-SLSS 146

Query: 126 FLLLTLSPLMMSPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKEEEQSCGFGK 185
            LLL  S +++  W  +   +    D         AF  A+   +++Y+  ++Q   F K
Sbjct: 147 LLLLLHSQVILKTWTNLKDVILEGKD---------AFSSAHDMRLFEYISSDDQ---FSK 206

Query: 186 LFHQTLECDSRLIGKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFPHINCFVLDLP 245
           LFH+ +   S ++ K V  E     E + +LVDVGGG G +   I   +PHI     DL 
Sbjct: 207 LFHRAMSESSTMVMKKVLEEY-RGFEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLA 266

Query: 246 EVVANQKPKQQIQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKILKNCKEAIPSR 305
           +V+  Q P      +  + GDMF ++P  +A+ +K ILHDW DE+ IKILKNC +++P  
Sbjct: 267 QVL-TQAP--FYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLP-- 326

Query: 306 EEGGKVIIIEMVLEKELEEMKKSSVETETKLCCDVLMMTIFS-GRERNEKEWNNLFLAAG 355
            E GKVII+EM+      E K +     T L  D+LM+T  S G+ER+  ++ NL  A+G
Sbjct: 327 -EKGKVIIVEMITPM---EPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASG 368

BLAST of Chy4G080670 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 139.0 bits (349), Expect = 7.4e-33
Identity = 89/270 (32.96%), Postives = 151/270 (55.93%), Query Frame = 0

Query: 100 NKTAFDTSPFLLLTLSPLMMSPWQTMSKWLCSQDDDQHQDHYSTAFEMANGKSIWDYVKE 159
           ++  F  +P++L   +      W  +++ +        Q+  ++A+E AN   I++Y+K+
Sbjct: 115 DEDGFSIAPYVLAGCTKAKGGVWSYLTEAI--------QEGGASAWERANEALIFEYMKK 174

Query: 160 EEQSCGFGKLFHQTLECDSRLI-GKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEAFP 219
            E      K+F++++   + ++  K++ +  G   EG+   VDVGG  G+    I+  +P
Sbjct: 175 NE---NLKKIFNESMTNHTSIVMKKILENYIG--FEGVSDFVDVGGSLGSNLAQILSKYP 234

Query: 220 HINCFVLDLPEVVANQKPKQQIQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIKIL 279
           HI     DLP +V   K   QI  +  I GDMF +IP    +L+K ILHDWNDE+ ++IL
Sbjct: 235 HIKGINFDLPHIV---KEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEIL 294

Query: 280 KNCKEAIPSREEGGKVIIIEMVLEKELEEMKKSSVETETKLCCDVLMMTIFS-GRERNEK 339
           KNCK+A+P   E G++I+IEM++ +E+ E   + + T+  L  D+ MM++ S G+ER +K
Sbjct: 295 KNCKKALP---ETGRIIVIEMIVPREVSE---TDLATKNSLSADLTMMSLTSGGKERTKK 354

Query: 340 EWNNLFLAAGFAHYNIIPILGLRSLIVVYP 368
           E+ +L   AGF    II       +I +YP
Sbjct: 355 EFEDLAKEAGFKLPKIIYGAYSYWIIELYP 362

BLAST of Chy4G080670 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 137.5 bits (345), Expect = 2.2e-32
Identity = 91/272 (33.46%), Postives = 150/272 (55.15%), Query Frame = 0

Query: 100 NKTAFDTSPFLLLTLSPLMMSPWQTMSKWLCSQDDDQH--QDHYSTAFEMANGKSIWDYV 159
           ++  F  +P++L   +      W  +          QH  Q+  ++A+E AN   I++Y+
Sbjct: 115 DEDGFSIAPYVLAGCTKAKGGVWYNV----------QHAIQEGGASAWERANEALIFEYM 174

Query: 160 KEEEQSCGFGKLFHQTLECDSRLI-GKVVSSECGEMLEGLRSLVDVGGGAGAMAKAIVEA 219
           K+ E      K+F++++   + ++  K++ +  G   EG+   VDVGG  G+    I+  
Sbjct: 175 KKNE---NLKKIFNESMTNHTSIVMKKILENYIG--FEGVSDFVDVGGSLGSNLAQILSK 234

Query: 220 FPHINCFVLDLPEVVANQKPKQQIQNLHFIVGDMFQKIPPANAVLLKSILHDWNDEESIK 279
           +PHI     DLP +V   K   QI  +  I GDMF +IP    +L+K ILHDWNDE+ ++
Sbjct: 235 YPHIKGINFDLPHIV---KEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVE 294

Query: 280 ILKNCKEAIPSREEGGKVIIIEMVLEKELEEMKKSSVETETKLCCDVLMMTIFS-GRERN 339
           ILKNCK+A+P   E G++I+IEM++ +E+ E   + + T+  L  D+ MM++ S G+ER 
Sbjct: 295 ILKNCKKALP---ETGRIIVIEMIVPREVSE---TDLATKNSLSADLTMMSLTSGGKERT 354

Query: 340 EKEWNNLFLAAGFAHYNIIPILGLRSLIVVYP 368
           +KE+ +L   AGF    II       +I +YP
Sbjct: 355 KKEFEDLAKEAGFKLPKIIYGAYSYWIIELYP 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
B6VJS47.4e-9450.27Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1... [more]
B0ZB576.9e-9249.07Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1[more]
A0A0N9HMN65.1e-8749.19Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P... [more]
Q6VMW03.1e-8445.628-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=... [more]
Q93WU22.6e-8346.34Eugenol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=EOMT1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3CHG43.4e-16692.72Myricetin O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1F4795.8e-14271.04probable O-methyltransferase 3 OS=Cucurbita moschata OX=3662 GN=LOC111442010 PE=... [more]
A0A6J1J5U87.5e-14271.04probable O-methyltransferase 3 OS=Cucurbita maxima OX=3661 GN=LOC111481541 PE=3 ... [more]
A0A1S3CNB01.4e-14092.99myricetin O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103502839 PE=3 ... [more]
A0A6J1E0963.1e-12765.86probable O-methyltransferase 3 OS=Momordica charantia OX=3673 GN=LOC111025195 PE... [more]
Match NameE-valueIdentityDescription
KAA0037695.16.16e-21392.72myricetin O-methyltransferase-like [Cucumis melo var. makuwa] >TYK10678.1 myrice... [more]
XP_038905391.12.75e-19876.29trans-resveratrol di-O-methyltransferase-like [Benincasa hispida][more]
XP_023528438.13.23e-18271.58probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo][more]
XP_022935024.14.35e-18071.04probable O-methyltransferase 3 [Cucurbita moschata][more]
XP_023520752.14.35e-18071.04probable O-methyltransferase 3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT4G35160.12.1e-4832.35O-methyltransferase family protein [more]
AT4G35150.16.3e-4030.52O-methyltransferase family protein [more]
AT1G77530.12.6e-3331.06O-methyltransferase family protein [more]
AT1G51990.17.4e-3332.96O-methyltransferase family protein [more]
AT1G51990.22.2e-3233.46O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 138..349
e-value: 4.3E-54
score: 183.0
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 25..73
e-value: 3.5E-19
score: 68.6
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 2..95
e-value: 2.3E-39
score: 135.2
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 101..367
e-value: 1.8E-73
score: 248.8
NoneNo IPR availablePIRSRPIRSR005739-1PIRSR005739-1coord: 24..366
e-value: 2.6E-80
score: 267.5
NoneNo IPR availablePANTHERPTHR11746:SF250O-METHYLTRANSFERASEcoord: 6..367
IPR016461O-methyltransferase COMT-typePIRSFPIRSF005739O-mtasecoord: 1..367
e-value: 1.9E-117
score: 390.2
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 6..367
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 15..367
score: 73.42038
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 6..98
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 107..365

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G080670.1Chy4G080670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019438 aromatic compound biosynthetic process
biological_process GO:0032259 methylation
molecular_function GO:0008171 O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity