Chy4G079480 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G079480
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	chrH04: 18830822 .. 18831880 (+)
RNA-Seq Expression	Chy4G079480
Synteny	Chy4G079480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGAGAAATCATAAACTTAACCAAGCTATGGTTAACCGTCTGTATTTCTCTCAGTTACTGCCACGCCACCGCCAAACTCCTCAATCCCGGCCCCGCCCGCTTCCTTGCCATCGCTCCCGTCGTCCTCATCTTCTTCCTCCTCCCTCTCCAACTTACCTCCATCCACTTCGGCGGCGCCACCTGCTTCTTCGTTACTTGGCTCGCCACCTTCAAGCTTCTCCTCCTCACTGCCGGCGACGGCCCTCTCTCCACTACCCCGCCGCTTCCTCTCCACCGATTCATAGCCATATCTTGCTTGCCCATCAAGATCCTGGAAAACCCCTCGGAGAAATCGGCCCCCAAGAATAATCGAGCGGAAACCCTGAACGAAAAACCCAAATCGTTTCTCAACTACTCAATTAGAGCCTTAATCGTTGTTCTGATGGTGAAATTGTACGATTACTCTCAGTTTTTCCACCCGAAATTTGTTCTGTTCATTTATAGTTGGCACGTTTACCTTCTCCTTGAGATTATTCTGGCGATTACGCAGTTTTTTGGTCGTAATCTGCTCGGAATCGAGCTGGAACCGCAATTTCGGGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGGCGGTTTACAAGCCGACGGTGAAGATAGCTTCTAAGGTAATAGGGAGGATGTGGGCGCCGTTGCCGGGGGTGATGGCGACGTTCTTGGTGTCGGCAATGATGCACGAACTGATATTTTATTATTTGGGGAGAATGAGGCCGAACTGGGAGATCACGTGGTTCTTTGTTATACATGGAATTGCTTTGACGGTGGAGATTATGATGAAGAAGATGTTGCCAGAGAAGCGGATGTTACCGGCAAAGGTGTCGGTGCCGGTGACTCTAGTTTTCATATTCTGGACGGCGATTTGGTTGTTCTTTCCGCAGTTCGTGAGATTGAAGTTGGACGTGAGAGCGTTTGAAGAATACGCCGCGTTGGCTAATTTTGTCAAGAAAGTGGCTTTCTCATTGGTCGGTTGA

mRNA sequence

ATGGAGGGAGAAATCATAAACTTAACCAAGCTATGGTTAACCGTCTGTATTTCTCTCAGTTACTGCCACGCCACCGCCAAACTCCTCAATCCCGGCCCCGCCCGCTTCCTTGCCATCGCTCCCGTCGTCCTCATCTTCTTCCTCCTCCCTCTCCAACTTACCTCCATCCACTTCGGCGGCGCCACCTGCTTCTTCGTTACTTGGCTCGCCACCTTCAAGCTTCTCCTCCTCACTGCCGGCGACGGCCCTCTCTCCACTACCCCGCCGCTTCCTCTCCACCGATTCATAGCCATATCTTGCTTGCCCATCAAGATCCTGGAAAACCCCTCGGAGAAATCGGCCCCCAAGAATAATCGAGCGGAAACCCTGAACGAAAAACCCAAATCGTTTCTCAACTACTCAATTAGAGCCTTAATCGTTGTTCTGATGGTGAAATTGTACGATTACTCTCAGTTTTTCCACCCGAAATTTGTTCTGTTCATTTATAGTTGGCACGTTTACCTTCTCCTTGAGATTATTCTGGCGATTACGCAGTTTTTTGGTCGTAATCTGCTCGGAATCGAGCTGGAACCGCAATTTCGGGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGGCGGTTTACAAGCCGACGGTGAAGATAGCTTCTAAGGTAATAGGGAGGATGTGGGCGCCGTTGCCGGGGGTGATGGCGACGTTCTTGGTGTCGGCAATGATGCACGAACTGATATTTTATTATTTGGGGAGAATGAGGCCGAACTGGGAGATCACGTGGTTCTTTGTTATACATGGAATTGCTTTGACGGTGGAGATTATGATGAAGAAGATGTTGCCAGAGAAGCGGATGTTACCGGCAAAGGTGTCGGTGCCGGTGACTCTAGTTTTCATATTCTGGACGGCGATTTGGTTGTTCTTTCCGCAGTTCGTGAGATTGAAGTTGGACGTGAGAGCGTTTGAAGAATACGCCGCGTTGGCTAATTTTGTCAAGAAAGTGGCTTTCTCATTGGTCGGTTGA

Coding sequence (CDS)

ATGGAGGGAGAAATCATAAACTTAACCAAGCTATGGTTAACCGTCTGTATTTCTCTCAGTTACTGCCACGCCACCGCCAAACTCCTCAATCCCGGCCCCGCCCGCTTCCTTGCCATCGCTCCCGTCGTCCTCATCTTCTTCCTCCTCCCTCTCCAACTTACCTCCATCCACTTCGGCGGCGCCACCTGCTTCTTCGTTACTTGGCTCGCCACCTTCAAGCTTCTCCTCCTCACTGCCGGCGACGGCCCTCTCTCCACTACCCCGCCGCTTCCTCTCCACCGATTCATAGCCATATCTTGCTTGCCCATCAAGATCCTGGAAAACCCCTCGGAGAAATCGGCCCCCAAGAATAATCGAGCGGAAACCCTGAACGAAAAACCCAAATCGTTTCTCAACTACTCAATTAGAGCCTTAATCGTTGTTCTGATGGTGAAATTGTACGATTACTCTCAGTTTTTCCACCCGAAATTTGTTCTGTTCATTTATAGTTGGCACGTTTACCTTCTCCTTGAGATTATTCTGGCGATTACGCAGTTTTTTGGTCGTAATCTGCTCGGAATCGAGCTGGAACCGCAATTTCGGGATCCGTACTATTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGGCGGTTTACAAGCCGACGGTGAAGATAGCTTCTAAGGTAATAGGGAGGATGTGGGCGCCGTTGCCGGGGGTGATGGCGACGTTCTTGGTGTCGGCAATGATGCACGAACTGATATTTTATTATTTGGGGAGAATGAGGCCGAACTGGGAGATCACGTGGTTCTTTGTTATACATGGAATTGCTTTGACGGTGGAGATTATGATGAAGAAGATGTTGCCAGAGAAGCGGATGTTACCGGCAAAGGTGTCGGTGCCGGTGACTCTAGTTTTCATATTCTGGACGGCGATTTGGTTGTTCTTTCCGCAGTTCGTGAGATTGAAGTTGGACGTGAGAGCGTTTGAAGAATACGCCGCGTTGGCTAATTTTGTCAAGAAAGTGGCTTTCTCATTGGTCGGTTGA

Protein sequence

MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGGATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRAETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAPLPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG*

Homology

[Close]

BLAST of Chy4G079480 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.1e-76
Identity = 156/338 (46.15%), Postives = 216/338 (63.91%), Query Frame = 0


Query: 10  KLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGGATCFFVTWL 69
           K W  V ISL Y    AKL+  G  R +   PV LIFF++P  + S+H  G T FF+ WL
Sbjct: 6   KAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWL 65

Query: 70  ATFKLLLLTAGDGPLSTT-PPLPLHRFIAISCLPIKILENPSEKSAPKNNRAETLNEKPK 129
           A FKLLL   G GPLS+   PL L  F+A+SCLPIKI      + +PK  +  +     +
Sbjct: 66  ANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI------QLSPKPTKTHSHEGSTE 125

Query: 130 SFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFFGRNLLGIE 189
             L Y+I+A+ VVL++K Y+YS     K VL +Y+ H+Y  LEIILA T    R +  +E
Sbjct: 126 GPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLE 185

Query: 190 LEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAPLPGVMATF 249
           LEPQF  PY +TSLQDFWGRRWNLM T IL+P VY+P++++ S V+G  ++ +     TF
Sbjct: 186 LEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTF 245

Query: 250 LVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPAKVSVPVTL 309
           +VS +MHELIF+Y+GR+RP+W++ WFF+I+G   TVEI +KK +  +   P  +S  +TL
Sbjct: 246 VVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTL 305

Query: 310 VFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKV 347
            F+  TA+WLF P+F R  +  +A +EYAA+  F  +V
Sbjct: 306 TFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of Chy4G079480 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 4.0e-65
Identity = 141/349 (40.40%), Postives = 206/349 (59.03%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           M+ E+ NL K+W++  IS+SYC+     +  G  RFL+++PV+ +F +LPL  +S+H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLH--RFIAISCLPIKILENPSEKSAPKNN 120
            T FF+TWLA FKL+L +   GPL    P+P +  RF   +C PIK+ +NP  +      
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQNPKSQ------ 120

Query: 121 RAETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQ 180
                N  PK  L ++I+  I  +++ LY Y Q   P  +L +Y  H+YL +EIIL   +
Sbjct: 121 -----NHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVK 180

Query: 181 FFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMW 240
                 LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+PAVY P  +++ + +   W
Sbjct: 181 VVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGW 240

Query: 241 APLPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKK--MLPEKR 300
           A  PG++A F+VS ++HEL+F+YL R  P  E+T FFV+HG+   VE+ +KK   + ++ 
Sbjct: 241 ALFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRW 300

Query: 301 MLPAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKK 346
            L   VS  +T+ F+F T  WLF PQ  R  +  R   E   L  F+K+
Sbjct: 301 RLSPGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of Chy4G079480 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 1.7e-63
Identity = 139/351 (39.60%), Postives = 203/351 (57.83%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           ME E+++L K+W+   IS+SYC+ T+  +  G  R L++ PV ++F +LPL ++S+HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           +T FF++WLA FKL+L +   GPL   P   L RF+  +C PIK+ +NP  +        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNPKPQ-------- 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
              N+ PK    ++++     +++ +Y+Y Q   P  +L +YS H+YL  EI+LA  +  
Sbjct: 121 ---NQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVL 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
               L  +LEP F +PY STSLQDFWGRRWNLM   IL+PAVY P  ++A + +    A 
Sbjct: 181 LSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQAL 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKK-MLPEKRMLP 300
             GV A+FLVS ++HELIF+Y  R  P  E+T FFV+HG+    E   K+  L  +  + 
Sbjct: 241 FLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVS 300

Query: 301 AKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSL 351
             VS  +T+ F+  T  WLFFP   R  +  R  +E      FVK   F L
Sbjct: 301 QMVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of Chy4G079480 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 4.9e-63
Identity = 138/354 (38.98%), Postives = 210/354 (59.32%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCH-ATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFG 60
           +E E+   +++W++   +  YC    A   + G  R L + PVVL+F  LPL+L+S H G
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHLG 62

Query: 61  GATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNR 120
           G T  ++ WLA FKLLL     GPLS  P L L  FI+ + LPIK  ++PS     KN R
Sbjct: 63  GPTALYLVWLANFKLLLFAFHLGPLS-NPSLSLLHFISTTLLPIKFRDDPS-NDHEKNKR 122

Query: 121 AETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVL-FIYSWHVYLLLEIILAITQ 180
             TL+ + +  + +  + +    ++K+Y++ +   P FV+  +Y +H YL  EI LA + 
Sbjct: 123 --TLSFEWRKVVLFVAKLVFFAGILKIYEFRKDL-PHFVISVLYCFHFYLGTEITLAASA 182

Query: 181 FFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMW 240
              R  LG++L PQF +PY +TSLQDFWGRRWNLM ++IL    Y+P  ++ S+ +   W
Sbjct: 183 VIARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRW 242

Query: 241 APLPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRML 300
                ++  F VS +MHE+ F+YL R RP+WE+T FFV+HG+   VE+++KK +  K  L
Sbjct: 243 EVAGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRL 302

Query: 301 PAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 353
             +VS  +T+ F+  T  WLF PQ VR  +D++  +EY  + N+ +K    L+G
Sbjct: 303 RREVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLMGLLG 351

[Close]

BLAST of Chy4G079480 vs. ExPASy Swiss-Prot
Match: Q9FJ74 (Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=AT3 PE=3 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.5e-61
Identity = 138/348 (39.66%), Postives = 204/348 (58.62%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           ME E+ N  KLW++  IS+SYC+  +  +  G  R L++ PV  +F + PL  + +HF G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLP--LHRFIAISCLPIKILENPSEKSAPKNN 120
              FF++WLA FKL+L +   GPLS   PLP  L RFI I+C PIK  +NP+ +      
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNPNIQ------ 120

Query: 121 RAETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQ 180
                N K   +L ++I+ +I V+++++Y+Y Q+  P  +L   S H++L LEI+  + +
Sbjct: 121 -----NYKIPIWL-FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVK 180

Query: 181 FFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMW 240
                 LG +LEPQ  +PY +TSLQDFWGRRWNLM   IL+PAVY P  ++A + +    
Sbjct: 181 ALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQ 240

Query: 241 APLPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKR-- 300
           A   GV A FLVS  +HE++F+YL R  P  E+TWFF++HG+    E+ +KK    +R  
Sbjct: 241 AMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWW 300

Query: 301 MLPAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVK 345
            +   VS  +T+ F+  T+ W FFP  +R  +  R   E     +FVK
Sbjct: 301 RVSPTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of Chy4G079480 vs. ExPASy TrEMBL
Match: A0A0A0L9S2 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=3 SV=1)

HSP 1 Score: 675.6 bits (1742), Expect = 1.1e-190
Identity = 341/352 (96.88%), Postives = 345/352 (98.01%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           ME EIINLTK+WLTV ISL YCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           ETLNEKPKSFLNYSIRALIVVLMVKLYDYS FFHPK VLFIYSWHVYLLLEIILAITQFF
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIA++VIGRMWAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKR LPA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 353
           KVSVPVTLVFIF TAIWLFFPQFVRL+LDVRAFEEYAALANFVKKVAFSLVG
Sbjct: 328 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 379

[Close]

BLAST of Chy4G079480 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.4e-161
Identity = 281/351 (80.06%), Postives = 315/351 (89.74%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           MEGEIIN T +WL VC+SL YCH   K+L+PG  RFLAI+PVVL+FFLLPLQLTS+H GG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           AT FF+ WLA FKLLL  AG+GPLS TPPLPL RF+A+SCLPIKIL+NPSEK +PKN+  
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHH- 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           ETLN++PKSFLNY I+ALIV LMVKLYDYS+F HPK +LFIYSWHVY  LEIILAITQFF
Sbjct: 121 ETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GR LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP VYKPTVKIA+KV+GR WAP
Sbjct: 181 GRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LPGVMATFLVSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEI++KK+LPEKR LPA
Sbjct: 241 LPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLV 352
            VSVPVTLVFIF TAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF LV
Sbjct: 301 AVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

[Close]

BLAST of Chy4G079480 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 577.8 bits (1488), Expect = 3.1e-161
Identity = 287/352 (81.53%), Postives = 313/352 (88.92%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           MEGEI+N  K+WLTVCISL YCHAT KLL PGP RFLA+AP+V++FFLLPLQLTSIH GG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           +T FF+ WLA FKLLL  AGDGPLS  PPLPL RF+A+SCLPIKIL+N  EKS P NNR+
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           +T   KPKSFLNYSI+A+IV LMVKLYDYS+F HPK VLFIYSWH+Y LLEIILAITQFF
Sbjct: 121 DT-TLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GRN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQP VYKP+VKIA+KVIGR WAP
Sbjct: 181 GRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LP VMATFLVSAMMHELIFYY+GRMRPNWE TWFFVIHG+ALTVEIM+KK+LPEK  LP 
Sbjct: 241 LPAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPP 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 353
            VSVPVTL FIF TAIWLFFPQFVRLKLDVRAFEEYAAL  FVK VAFSLVG
Sbjct: 301 AVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of Chy4G079480 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 1.5e-160
Identity = 278/351 (79.20%), Postives = 314/351 (89.46%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           MEGE+IN   +WL VC+SL YCH   K+L PG  RFLAIAPVV++FFLLPLQLTS+H GG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           +T FF+ WLA FKLLL TAG+GPLS TPPLPL RF+A+SCLPIKIL+NPSEK +PKN+  
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHH- 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           ETLN++PKSFLNY I+ALIV LMVKLYDYS+F HPK +LFIYSWHVY  LEIILAITQFF
Sbjct: 121 ETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GR LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP +YKPTVKIA+KV+GR WAP
Sbjct: 181 GRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LPGVMATFLVSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEI++KK+LPEKR LPA
Sbjct: 241 LPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLV 352
            VSVPVTLVFIF TAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF LV
Sbjct: 301 AVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of Chy4G079480 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 1.0e-159
Identity = 284/352 (80.68%), Postives = 312/352 (88.64%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           MEGEI+N  K+WLTVCISL YCHAT KL+ PG  RFLA+AP+V++FF LPLQLTSIH GG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           +T FF+ WLA FKLLL TAGDGPLS  PPLPL RF+A+SCLPIKIL+N  EKS P NNR+
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           +T   KPKSFLNYSI+A+IV LMVKLYDYS+F HPK VLFIYSWH+Y LLEIILAITQFF
Sbjct: 121 DT-TLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GRN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQP VYKP+VKIA+KVIGR WAP
Sbjct: 181 GRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LP VMATFLVSAMMHELIF+Y+GRMRPNWE TWFFVIHG+ALTVEIM+KK+LPEK  LP 
Sbjct: 241 LPAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPP 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 353
            VSVPVTL FIF TAIWLFFPQFVRLKLDVRAFEEYAAL  FVK VAFSLVG
Sbjct: 301 AVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of Chy4G079480 vs. NCBI nr
Match: XP_004149740.1 (acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus])

HSP 1 Score: 675 bits (1741), Expect = 3.45e-244
Identity = 341/352 (96.88%), Postives = 345/352 (98.01%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           ME EIINLTK+WLTV ISL YCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG
Sbjct: 1   MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA
Sbjct: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           ETLNEKPKSFLNYSIRALIVVLMVKLYDYS FFHPK VLFIYSWHVYLLLEIILAITQFF
Sbjct: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIA++VIGRMWAP
Sbjct: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKR LPA
Sbjct: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 352
           KVSVPVTLVFIF TAIWLFFPQFVRL+LDVRAFEEYAALANFVKKVAFSLVG
Sbjct: 301 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 352

[Close]

BLAST of Chy4G079480 vs. NCBI nr
Match: KGN58563.1 (hypothetical protein Csa_002202 [Cucumis sativus])

HSP 1 Score: 675 bits (1741), Expect = 9.46e-244
Identity = 341/352 (96.88%), Postives = 345/352 (98.01%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           ME EIINLTK+WLTV ISL YCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           ETLNEKPKSFLNYSIRALIVVLMVKLYDYS FFHPK VLFIYSWHVYLLLEIILAITQFF
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIA++VIGRMWAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKR LPA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 352
           KVSVPVTLVFIF TAIWLFFPQFVRL+LDVRAFEEYAALANFVKKVAFSLVG
Sbjct: 328 KVSVPVTLVFIFSTAIWLFFPQFVRLRLDVRAFEEYAALANFVKKVAFSLVG 379

[Close]

BLAST of Chy4G079480 vs. NCBI nr
Match: XP_023527814.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 578 bits (1489), Expect = 8.24e-206
Identity = 281/351 (80.06%), Postives = 314/351 (89.46%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           MEGEIIN T +WL VC+SL YCH   K+L PG  RFLAIAPVVL+FFLLPLQLTS+H GG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           AT FF+ WLA FKLLL  AG+GPLS TPPLPL RF+A+SCLPIKIL+NPSEK +PKN+  
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHH- 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           ETLN++PKSFLNY I+ALIV LMVKLYDYS+F HPK +LFIYSWHVY  LEIILAITQFF
Sbjct: 121 ETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GR LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP +YKPTVKIA+KV+GR WAP
Sbjct: 181 GRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LPGVMATFLVSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEI++KK+LPEKR LPA
Sbjct: 241 LPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLV 351
            VSVPVTLVFIF TAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF LV
Sbjct: 301 AVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFIKKLAFPLV 350

[Close]

BLAST of Chy4G079480 vs. NCBI nr
Match: XP_022982638.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 577 bits (1488), Expect = 1.17e-205
Identity = 281/351 (80.06%), Postives = 315/351 (89.74%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           MEGEIIN T +WL VC+SL YCH   K+L+PG  RFLAI+PVVL+FFLLPLQLTS+H GG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           AT FF+ WLA FKLLL  AG+GPLS TPPLPL RF+A+SCLPIKIL+NPSEK +PKN+  
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHH- 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           ETLN++PKSFLNY I+ALIV LMVKLYDYS+F HPK +LFIYSWHVY  LEIILAITQFF
Sbjct: 121 ETLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GR LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQP VYKPTVKIA+KV+GR WAP
Sbjct: 181 GRTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LPGVMATFLVSA+MHELIFYYLGRM+PNWE+TWFFVIHG ALTVEI++KK+LPEKR LPA
Sbjct: 241 LPGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPA 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLV 351
            VSVPVTLVFIF TAIWLFFPQF+RLK+DV+AFEEYAAL +F+KK+AF LV
Sbjct: 301 AVSVPVTLVFIFSTAIWLFFPQFMRLKIDVKAFEEYAALVSFMKKLAFPLV 350

[Close]

BLAST of Chy4G079480 vs. NCBI nr
Match: XP_022948970.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577 bits (1487), Expect = 1.54e-205
Identity = 287/352 (81.53%), Postives = 313/352 (88.92%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           MEGEI+N  K+WLTVCISL YCHAT KLL PGP RFLA+AP+V++FFLLPLQLTSIH GG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           +T FF+ WLA FKLLL  AGDGPLS  PPLPL RF+A+SCLPIKIL+N  EKS P NNR+
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
           +T   KPKSFLNYSI+A+IV LMVKLYDYS+F HPK VLFIYSWH+Y LLEIILAITQFF
Sbjct: 121 DT-TLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFF 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
           GRN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQP VYKP+VKIA+KVIGR WAP
Sbjct: 181 GRNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAP 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPA 300
           LP VMATFLVSAMMHELIFYY+GRMRPNWE TWFFVIHG+ALTVEIM+KK+LPEK  LP 
Sbjct: 241 LPAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPP 300

Query: 301 KVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSLVG 352
            VSVPVTL FIF TAIWLFFPQFVRLKLDVRAFEEYAAL  FVK VAFSLVG
Sbjct: 301 AVSVPVTLAFIFPTAIWLFFPQFVRLKLDVRAFEEYAALVTFVKNVAFSLVG 349

[Close]

BLAST of Chy4G079480 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 286.2 bits (731), Expect = 3.6e-77
Identity = 156/338 (46.15%), Postives = 216/338 (63.91%), Query Frame = 0


Query: 10  KLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGGATCFFVTWL 69
           K W  V ISL Y    AKL+  G  R +   PV LIFF++P  + S+H  G T FF+ WL
Sbjct: 6   KAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWL 65

Query: 70  ATFKLLLLTAGDGPLSTT-PPLPLHRFIAISCLPIKILENPSEKSAPKNNRAETLNEKPK 129
           A FKLLL   G GPLS+   PL L  F+A+SCLPIKI      + +PK  +  +     +
Sbjct: 66  ANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKI------QLSPKPTKTHSHEGSTE 125

Query: 130 SFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFFGRNLLGIE 189
             L Y+I+A+ VVL++K Y+YS     K VL +Y+ H+Y  LEIILA T    R +  +E
Sbjct: 126 GPLIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLE 185

Query: 190 LEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAPLPGVMATF 249
           LEPQF  PY +TSLQDFWGRRWNLM T IL+P VY+P++++ S V+G  ++ +     TF
Sbjct: 186 LEPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTF 245

Query: 250 LVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRMLPAKVSVPVTL 309
           +VS +MHELIF+Y+GR+RP+W++ WFF+I+G   TVEI +KK +  +   P  +S  +TL
Sbjct: 246 VVSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTL 305

Query: 310 VFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKV 347
            F+  TA+WLF P+F R  +  +A +EYAA+  F  +V
Sbjct: 306 TFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAFAVEV 336

[Close]

BLAST of Chy4G079480 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 250.0 bits (637), Expect = 2.9e-66
Identity = 141/349 (40.40%), Postives = 206/349 (59.03%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           M+ E+ NL K+W++  IS+SYC+     +  G  RFL+++PV+ +F +LPL  +S+H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLH--RFIAISCLPIKILENPSEKSAPKNN 120
            T FF+TWLA FKL+L +   GPL    P+P +  RF   +C PIK+ +NP  +      
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLI---PIPTNFPRFFCFTCFPIKVQQNPKSQ------ 120

Query: 121 RAETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQ 180
                N  PK  L ++I+  I  +++ LY Y Q   P  +L +Y  H+YL +EIIL   +
Sbjct: 121 -----NHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVK 180

Query: 181 FFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMW 240
                 LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+PAVY P  +++ + +   W
Sbjct: 181 VVVFISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGW 240

Query: 241 APLPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKK--MLPEKR 300
           A  PG++A F+VS ++HEL+F+YL R  P  E+T FFV+HG+   VE+ +KK   + ++ 
Sbjct: 241 ALFPGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRW 300

Query: 301 MLPAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKK 346
            L   VS  +T+ F+F T  WLF PQ  R  +  R   E   L  F+K+
Sbjct: 301 RLSPGVSRVLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIKR 333

[Close]

BLAST of Chy4G079480 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 244.6 bits (623), Expect = 1.2e-64
Identity = 139/351 (39.60%), Postives = 203/351 (57.83%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           ME E+++L K+W+   IS+SYC+ T+  +  G  R L++ PV ++F +LPL ++S+HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
           +T FF++WLA FKL+L +   GPL   P   L RF+  +C PIK+ +NP  +        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNPKPQ-------- 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
              N+ PK    ++++     +++ +Y+Y Q   P  +L +YS H+YL  EI+LA  +  
Sbjct: 121 ---NQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVL 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
               L  +LEP F +PY STSLQDFWGRRWNLM   IL+PAVY P  ++A + +    A 
Sbjct: 181 LSISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQAL 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKK-MLPEKRMLP 300
             GV A+FLVS ++HELIF+Y  R  P  E+T FFV+HG+    E   K+  L  +  + 
Sbjct: 241 FLGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVS 300

Query: 301 AKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVKKVAFSL 351
             VS  +T+ F+  T  WLFFP   R  +  R  +E      FVK   F L
Sbjct: 301 QMVSRLLTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKFFYL 337

[Close]

BLAST of Chy4G079480 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 236.9 bits (603), Expect = 2.5e-62
Identity = 138/348 (39.66%), Postives = 204/348 (58.62%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           ME E+ N  KLW++  IS+SYC+  +  +  G  R L++ PV  +F + PL  + +HF G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLP--LHRFIAISCLPIKILENPSEKSAPKNN 120
              FF++WLA FKL+L +   GPLS   PLP  L RFI I+C PIK  +NP+ +      
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNPNIQ------ 120

Query: 121 RAETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQ 180
                N K   +L ++I+ +I V+++++Y+Y Q+  P  +L   S H++L LEI+  + +
Sbjct: 121 -----NYKIPIWL-FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVK 180

Query: 181 FFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMW 240
                 LG +LEPQ  +PY +TSLQDFWGRRWNLM   IL+PAVY P  ++A + +    
Sbjct: 181 ALVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQ 240

Query: 241 APLPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKR-- 300
           A   GV A FLVS  +HE++F+YL R  P  E+TWFF++HG+    E+ +KK    +R  
Sbjct: 241 AMFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWW 300

Query: 301 MLPAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVK 345
            +   VS  +T+ F+  T+ W FFP  +R  +  R   E     +FVK
Sbjct: 301 RVSPTVSRLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVK 332

[Close]

BLAST of Chy4G079480 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 236.5 bits (602), Expect = 3.3e-62
Identity = 133/347 (38.33%), Postives = 200/347 (57.64%), Query Frame = 0


Query: 1   MEGEIINLTKLWLTVCISLSYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 60
           ME +  NL ++W++  ISLSYC+  +  L+ G  R L+I PV ++F +LPL L+ +HF  
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 120
            +  F++WLA FKLLL    +GPL   PP  L RFI  +CLPIKI ++PS  + P     
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIP----- 120

Query: 121 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSQFFHPKFVLFIYSWHVYLLLEIILAITQFF 180
             L+ KP      +++ L++ +++ +Y+Y        VL +Y  H+YL +E++L      
Sbjct: 121 -NLHPKPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAV 180

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIASKVIGRMWAP 240
              LLG  +EP F +PY +TSLQDFW RRWNLM + +L+  V+ P  +   +++    A 
Sbjct: 181 VSTLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAM 240

Query: 241 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKK---MLPEKRM 300
             GVMA+F VS +MHEL+++Y+ R  P WE+T FFV+HG A   EI +K+   + P  R 
Sbjct: 241 FAGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRTQWLRPPHR- 300

Query: 301 LPAKVSVPVTLVFIFWTAIWLFFPQFVRLKLDVRAFEEYAALANFVK 345
               VS  V L F+  T +WLF  Q +R  +  +A  E   + +  K
Sbjct: 301 ---AVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

The following BLAST results are available for this feature:

BLAST of Chy4G079480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	5.1e-76	46.15	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9FJ76	4.0e-65	40.40	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	1.7e-63	39.60	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9XGY6	4.9e-63	38.98	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]
Q9FJ74	3.5e-61	39.66	Probable long-chain-alcohol O-fatty-acyltransferase 3 OS=Arabidopsis thaliana OX...	[more]

BLAST of Chy4G079480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L9S2	1.1e-190	96.88	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=...	[more]
A0A6J1J5C4	2.4e-161	80.06	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1GBE5	3.1e-161	81.53	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1F6Y6	1.5e-160	79.20	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1KAA2	1.0e-159	80.68	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of Chy4G079480 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004149740.1	3.45e-244	96.88	acyl-CoA--sterol O-acyltransferase 1 [Cucumis sativus]	[more]
KGN58563.1	9.46e-244	96.88	hypothetical protein Csa_002202 [Cucumis sativus]	[more]
XP_023527814.1	8.24e-206	80.06	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982638.1	1.17e-205	80.06	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_022948970.1	1.54e-205	81.53	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acy...	[more]

BLAST of Chy4G079480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	3.6e-77	46.15	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	2.9e-66	40.40	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	1.2e-64	39.60	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	2.5e-62	39.66	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	3.3e-62	38.33	MBOAT (membrane bound O-acyl transferase) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..352 e-value: 3.2E-115 score: 382.9
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 190..276 e-value: 2.1E-15 score: 56.7
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 2..347
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..347

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G079480.1	Chy4G079480.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity