Chy4G074190 (gene) Cucumber (hystrix) v1

Overview
NameChy4G074190
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionOxoglutarate/iron-dependent dioxygenase
LocationchrH04: 8415193 .. 8418205 (-)
RNA-Seq ExpressionChy4G074190
SyntenyChy4G074190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATGACGGTGGCCCTAGATATGCTGGAAGAGGCCATCCGAATAACAGAGGACGATCTCCTCGCTCCGCCGATCATTTCACTTATCGTCCCCGTCATGTAATGTAACCTTCTTCAAGCTCCACCACTTTTGAAAAGTTTACACGCATATTGGCTCTAGTCTTTATGTTTCCATTCTTTGTTTGTTGATCTAATGTATTTATTCTCGTCTATGGAAAGATCGAGTTAACTGTTTACTGAGTGCGTTTAAGTTTTTTGTTTTTCTTTTTCGTTCATGCATTAGAATTCAATTATATTGTTGACACAATTAGAGATTCATCAGATATTGTAAAAATGTGATCAAACTTAAGGAAATGCTAAATTGCTCAGTTTTGTGTTTATTTGGACTCATTAATTTACAATGTGTCTGATAAGTTCATTGGCTAAGAGTTTTGTGTTTTATTGGTCCTTGATCTATCCACGTTTTTTTAAAAAAATCTCAGCAATATTGAAACTGTAGGGTAACGTGAATCATAAAATTTAAATTTATATCTATTAGAGGAATTACTTTTTAAAATTTTTGAATTTCAAAGACCTTTTGGATCCAAAAGTCTAGGGACTTGTTAAACTTTTGTTCATGTTCATTGACTTATTTCACACAAAATTTCAAGAACTTGTTCGACACTTTTTGTTGTTGAAGGACCAAATAGACACAAAATTTAAAGTTCAAGGACTAAACTTACCATTTAACTTAAGTAAATTGATCATTTGTAATGTGTGTATCTCGGTAATGAGCCCAGAGCTTCTGGTTTAATCTGTTTATGGAATTCTGAAATTTGTAGGTGTTTAGTTTTGATTCCAAATGAAAGAAATTCAGTGTATGTTAGGAATAGGCTGTTTCTGTTAGGTGGGAGTAGAATTGAAGGTGCTGGAGTTTATAACTGTACTCTCATGTTTACTTCTAATCTTTTCACATTCTAACAAAATGGGCAGGCTGGAGGTGAGGGGTTTTCTGTTGCTGGTTCTTCTAACCCTGGTAGTGGAGCTTTCCGAGGTAGAAGTTCTCACCAGATGTCATCAAGGAATTTCAGGAAGCCTGTTGGACAAAAACAGGCCTCAAGTTCTGAACAATGGCAGTGGCGGCCTTTAAATAGCGGGAAAGATGCATCTCCTGGTGCTGTTGATCTTCAGCTGCAACATAATTCAACTGATGATATGAGCAATACCAATAAACAATTATTGGGATCTATTGCATCGAATTCTGATAGTAATCAAATACGCAATACTTCAGAGCAACAATTGGGATCTATTGCATCAAATTCTGATTGCATCGAACTCTCATCCTCCTCTGCTCAAAATGTCTCTAAGAGTTTGCATTCTGCTGTAGAAAGAATTCAGATTCAGGGACCTACAGCAGTATGTGGAAGTTGTAGTGATTCTTTTCCTCATGATAATTGTAACAGATCAGATGTGGTTGGACAGGAACTCAAGGTTCAACCTTCATTGAAATCCTGTGCAAGAGATGAGAGTTTTACCATACAACTTGGGGAAAGTAATAATGTTTTTAACTCTACAGACTCAAAAGACAAGAAGCCTTCAGTAGACCTTGATTCTTTTGATATATGCCCTCCAAAAACTGGAGGTGTCATGCTGAATCCTCCTTTATTAGCTATGAACAGAGAAAAAAGAAATGAGATGAGGCGAGCAATGGAAGGAAATAATGGAATTGTGTTGAGACCAGGAATGGTTCATCTGAAGGGTGGAATTTCCCTCAGGGATCAGGTATGATTTCTCTCTCGCACTTGTTACTCTAGAAACAGTCTCACTCTCACTCTCACTCTCTGACATAACTGAATCAGTTCTTGAGTTAAGGATCTTTCCTTGAATCTCTAGGATGGAAAACTCAATCTCCAAGGATAGGATACCACAGTAGCATTTTTCTTTGTCTTGACCAAGCTTTCATGGAGTAAAACGAAATATGGCAGAATATATACAAGGCAAAGTTTTACAAAAGGAGTCCAAACACTAAGCACAATTGACTAACATTTAGATGATGATTACAAAAATCCTGAGGAAATATGATTTATTATCAAGCAAGAAATGGATTTTTATTAGAGGATGATTTGTAGCTTCCACTATGGTCTGTGTTCCATTTTCATTCTAGGTTTATCATACAGGTGTTGGGTGGTTTTCTTTATGTTTGATTTGAGCCACACTATTATTTCTAGATTTGTTGGGAGCCTTCTCAAAACTCTGAATCTTCCTTGTTTTTCTAACTGGTGCTTATCTATCTCTATTATGGTCACTGCAGGCGAAGATAGTAAAAAAATGTCGGGATCTTGGTATTGGAGCTGGAGGTTTTTACCAACCTGGTTACCGTGAAGGAGGAAAACTACACCTGAAAATGATGTGCCTAGGTAAAAATTGGGATCCTGACAGTAGTACATATGGAGATATTCGTCCATTTGATGATACAAAACCACCTAACCTACCAGATGAATTTTACCAACTGGTTCAAAAGGCAATCAAAGATTCTTATGCCATTATGGCGGAAGATTCAACAATAAAAAATCCTGAACGCGTACTTCCATGGATGAAACCGGACATTTGTATTGTAAACTTCTACTCACAAAATGGACGATTAGGTCTTCATCAGGTCTGCAATAATGGTTCCTATCCTCTTCACTTTTTGGATTGTCCAACATTTGCATAATTCTTCATTCTTCCATCATAATCTGTGTCATTTTCTTCTCATTAGGATCGAGATGAAAGTCAAGAAAGTCTTGATAAAGGATTGCCTGTCATCTCCTTCTCCATTGGTGACTCTGCTGAATTCCTATTTGGTGATCGGAGTGATGTTGATCAAGCAGAGAAAGTTACTTTGGAATCAGGAGATATCTTGATATTTGGTGGGAAATCAAGACATGTTTTCCATGGGGTGACTGCAATTCATTCAAACACTGCTCCAAAAGCACTTTTAGAAGCAACAAATCTTCGTCCAGGTCGCTTAAATCTTACTTTTCGTCAGTATTGA

mRNA sequence

ATGAATGACGGTGGCCCTAGATATGCTGGAAGAGGCCATCCGAATAACAGAGGACGATCTCCTCGCTCCGCCGATCATTTCACTTATCGTCCCCGTCATGCTGGAGGTGAGGGGTTTTCTGTTGCTGGTTCTTCTAACCCTGGTAGTGGAGCTTTCCGAGGTAGAAGTTCTCACCAGATGTCATCAAGGAATTTCAGGAAGCCTGTTGGACAAAAACAGGCCTCAAGTTCTGAACAATGGCAGTGGCGGCCTTTAAATAGCGGGAAAGATGCATCTCCTGGTGCTGTTGATCTTCAGCTGCAACATAATTCAACTGATGATATGAGCAATACCAATAAACAATTATTGGGATCTATTGCATCGAATTCTGATAGTAATCAAATACGCAATACTTCAGAGCAACAATTGGGATCTATTGCATCAAATTCTGATTGCATCGAACTCTCATCCTCCTCTGCTCAAAATGTCTCTAAGAGTTTGCATTCTGCTGTAGAAAGAATTCAGATTCAGGGACCTACAGCAGTATGTGGAAGTTGTAGTGATTCTTTTCCTCATGATAATTGTAACAGATCAGATGTGGTTGGACAGGAACTCAAGGTTCAACCTTCATTGAAATCCTGTGCAAGAGATGAGAGTTTTACCATACAACTTGGGGAAAGTAATAATGTTTTTAACTCTACAGACTCAAAAGACAAGAAGCCTTCAGTAGACCTTGATTCTTTTGATATATGCCCTCCAAAAACTGGAGGTGTCATGCTGAATCCTCCTTTATTAGCTATGAACAGAGAAAAAAGAAATGAGATGAGGCGAGCAATGGAAGGAAATAATGGAATTGTGTTGAGACCAGGAATGGTTCATCTGAAGGGTGGAATTTCCCTCAGGGATCAGGCGAAGATAGTAAAAAAATGTCGGGATCTTGGTATTGGAGCTGGAGGTTTTTACCAACCTGGTTACCGTGAAGGAGGAAAACTACACCTGAAAATGATGTGCCTAGGTAAAAATTGGGATCCTGACAGTAGTACATATGGAGATATTCGTCCATTTGATGATACAAAACCACCTAACCTACCAGATGAATTTTACCAACTGGTTCAAAAGGCAATCAAAGATTCTTATGCCATTATGGCGGAAGATTCAACAATAAAAAATCCTGAACGCGTACTTCCATGGATGAAACCGGACATTTGTATTGTAAACTTCTACTCACAAAATGGACGATTAGGTCTTCATCAGGATCGAGATGAAAGTCAAGAAAGTCTTGATAAAGGATTGCCTGTCATCTCCTTCTCCATTGGTGACTCTGCTGAATTCCTATTTGGTGATCGGAGTGATGTTGATCAAGCAGAGAAAGTTACTTTGGAATCAGGAGATATCTTGATATTTGGTGGGAAATCAAGACATGTTTTCCATGGGGTGACTGCAATTCATTCAAACACTGCTCCAAAAGCACTTTTAGAAGCAACAAATCTTCGTCCAGGTCGCTTAAATCTTACTTTTCGTCAGTATTGA

Coding sequence (CDS)

ATGAATGACGGTGGCCCTAGATATGCTGGAAGAGGCCATCCGAATAACAGAGGACGATCTCCTCGCTCCGCCGATCATTTCACTTATCGTCCCCGTCATGCTGGAGGTGAGGGGTTTTCTGTTGCTGGTTCTTCTAACCCTGGTAGTGGAGCTTTCCGAGGTAGAAGTTCTCACCAGATGTCATCAAGGAATTTCAGGAAGCCTGTTGGACAAAAACAGGCCTCAAGTTCTGAACAATGGCAGTGGCGGCCTTTAAATAGCGGGAAAGATGCATCTCCTGGTGCTGTTGATCTTCAGCTGCAACATAATTCAACTGATGATATGAGCAATACCAATAAACAATTATTGGGATCTATTGCATCGAATTCTGATAGTAATCAAATACGCAATACTTCAGAGCAACAATTGGGATCTATTGCATCAAATTCTGATTGCATCGAACTCTCATCCTCCTCTGCTCAAAATGTCTCTAAGAGTTTGCATTCTGCTGTAGAAAGAATTCAGATTCAGGGACCTACAGCAGTATGTGGAAGTTGTAGTGATTCTTTTCCTCATGATAATTGTAACAGATCAGATGTGGTTGGACAGGAACTCAAGGTTCAACCTTCATTGAAATCCTGTGCAAGAGATGAGAGTTTTACCATACAACTTGGGGAAAGTAATAATGTTTTTAACTCTACAGACTCAAAAGACAAGAAGCCTTCAGTAGACCTTGATTCTTTTGATATATGCCCTCCAAAAACTGGAGGTGTCATGCTGAATCCTCCTTTATTAGCTATGAACAGAGAAAAAAGAAATGAGATGAGGCGAGCAATGGAAGGAAATAATGGAATTGTGTTGAGACCAGGAATGGTTCATCTGAAGGGTGGAATTTCCCTCAGGGATCAGGCGAAGATAGTAAAAAAATGTCGGGATCTTGGTATTGGAGCTGGAGGTTTTTACCAACCTGGTTACCGTGAAGGAGGAAAACTACACCTGAAAATGATGTGCCTAGGTAAAAATTGGGATCCTGACAGTAGTACATATGGAGATATTCGTCCATTTGATGATACAAAACCACCTAACCTACCAGATGAATTTTACCAACTGGTTCAAAAGGCAATCAAAGATTCTTATGCCATTATGGCGGAAGATTCAACAATAAAAAATCCTGAACGCGTACTTCCATGGATGAAACCGGACATTTGTATTGTAAACTTCTACTCACAAAATGGACGATTAGGTCTTCATCAGGATCGAGATGAAAGTCAAGAAAGTCTTGATAAAGGATTGCCTGTCATCTCCTTCTCCATTGGTGACTCTGCTGAATTCCTATTTGGTGATCGGAGTGATGTTGATCAAGCAGAGAAAGTTACTTTGGAATCAGGAGATATCTTGATATTTGGTGGGAAATCAAGACATGTTTTCCATGGGGTGACTGCAATTCATTCAAACACTGCTCCAAAAGCACTTTTAGAAGCAACAAATCTTCGTCCAGGTCGCTTAAATCTTACTTTTCGTCAGTATTGA

Protein sequence

MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQMSSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIASNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCSDSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDSFDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIVKKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEFYQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESLDKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTAPKALLEATNLRPGRLNLTFRQY*
Homology
BLAST of Chy4G074190 vs. ExPASy Swiss-Prot
Match: P05050 (Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Escherichia coli (strain K12) OX=83333 GN=alkB PE=1 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 5.5e-15
Identity = 50/160 (31.25%), Postives = 77/160 (48.12%), Query Frame = 0

Query: 342 YGDIRPFDDTKPPNLPDEFYQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFY 401
           Y  I P  +   P +P  F+ L Q+A   +                 P  +PD C++N Y
Sbjct: 78  YSPIDPQTNKPWPAMPQSFHNLCQRAATAA---------------GYPDFQPDACLINRY 137

Query: 402 SQNGRLGLHQDRDESQESLDKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIF 461
           +   +L LHQD+DE     D   P++S S+G  A F FG     D  +++ LE GD++++
Sbjct: 138 APGAKLSLHQDKDEP----DLRAPIVSVSLGLPAIFQFGGLKRNDPLKRLLLEHGDVVVW 197

Query: 462 GGKSRHVFHGVTAIHSNTAPKALLEATNLRPGRLNLTFRQ 502
           GG+SR  +HG+  + +   P  +         R NLTFRQ
Sbjct: 198 GGESRLFYHGIQPLKAGFHPLTI-------DCRYNLTFRQ 211

BLAST of Chy4G074190 vs. ExPASy Swiss-Prot
Match: P37462 (Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) OX=99287 GN=alkB PE=3 SV=2)

HSP 1 Score: 82.8 bits (203), Expect = 1.2e-14
Identity = 56/172 (32.56%), Postives = 77/172 (44.77%), Query Frame = 0

Query: 331 LGKNWDPDSSTYGDIRPFDDTKPPNLPDEFYQLV-QKAIKDSYAIMAEDSTIKNPERVLP 390
           LG   D     Y    P  D   P LP  F  +  Q AI   YA                
Sbjct: 67  LGWTTDRHGYCYAVRDPLTDKPWPALPLSFASVCRQAAIAAGYAS--------------- 126

Query: 391 WMKPDICIVNFYSQNGRLGLHQDRDESQESLDKGLPVISFSIGDSAEFLFGDRSDVDQAE 450
             +PD C++N Y+   +L LHQD+DE     D   P++S S+G  A F FG     D  +
Sbjct: 127 -FQPDACLINRYAPGAKLSLHQDKDEP----DLRAPIVSVSLGVPAVFQFGGLRRSDPIQ 186

Query: 451 KVTLESGDILIFGGKSRHVFHGVTAIHSNTAPKALLEATNLRPGRLNLTFRQ 502
           ++ LE GDI+++GG+SR  +HG+  + +   P            R NLTFRQ
Sbjct: 187 RILLEHGDIVVWGGESRLFYHGIQPLKAGFHPMT-------GEFRYNLTFRQ 211

BLAST of Chy4G074190 vs. ExPASy Swiss-Prot
Match: P0CAT7 (Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 GN=alkB PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 6.0e-14
Identity = 47/115 (40.87%), Postives = 62/115 (53.91%), Query Frame = 0

Query: 389 PWMKPDICIVNFYSQNGRLGLHQDRDESQESLDKGLPVISFSIGDSAEFLFGDRSDVDQA 448
           P   PD C+VN Y    R+GLHQDRDE+    D   PV+S S+GD+A F  G  +  D  
Sbjct: 114 PETPPDSCLVNLYRDGARMGLHQDRDEA----DPRFPVLSISLGDTAVFRIGGVNRKDPT 173

Query: 449 EKVTLESGDILIFGGKSRHVFHGVTAIHSNTAPKALLEATNLRP--GRLNLTFRQ 502
             + L SGD+    G +R  FHGV  I        L  +++L P  GR+NLT R+
Sbjct: 174 RSLRLASGDVCRLLGPARLAFHGVDRI--------LPGSSSLVPGGGRINLTLRR 216

BLAST of Chy4G074190 vs. ExPASy Swiss-Prot
Match: B8GWW6 (Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides (strain NA1000 / CB15N) OX=565050 GN=alkB PE=3 SV=2)

HSP 1 Score: 80.5 bits (197), Expect = 6.0e-14
Identity = 47/115 (40.87%), Postives = 62/115 (53.91%), Query Frame = 0

Query: 389 PWMKPDICIVNFYSQNGRLGLHQDRDESQESLDKGLPVISFSIGDSAEFLFGDRSDVDQA 448
           P   PD C+VN Y    R+GLHQDRDE+    D   PV+S S+GD+A F  G  +  D  
Sbjct: 114 PETPPDSCLVNLYRDGARMGLHQDRDEA----DPRFPVLSISLGDTAVFRIGGVNRKDPT 173

Query: 449 EKVTLESGDILIFGGKSRHVFHGVTAIHSNTAPKALLEATNLRP--GRLNLTFRQ 502
             + L SGD+    G +R  FHGV  I        L  +++L P  GR+NLT R+
Sbjct: 174 RSLRLASGDVCRLLGPARLAFHGVDRI--------LPGSSSLVPGGGRINLTLRR 216

BLAST of Chy4G074190 vs. ExPASy Swiss-Prot
Match: Q54N08 (Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44689 GN=alkB PE=2 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 2.6e-09
Identity = 41/130 (31.54%), Postives = 68/130 (52.31%), Query Frame = 0

Query: 357 PDEFYQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDES 416
           PD+  +LVQK              I    +  P++  +   VNFYS++  +G H   D++
Sbjct: 240 PDDLQELVQK--------------IAIATKFDPYV-AEAATVNFYSEDSIMGGH--LDDA 299

Query: 417 QESLDKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIH 476
           ++ ++K  P+IS S G +A FL G  +       + + SGDI+I GG+SR+ +HGV  I 
Sbjct: 300 EQEMEK--PIISISFGSTAVFLMGAETRDIAPVPLFIRSGDIVIMGGRSRYCYHGVAKIV 350

Query: 477 SNTAPKALLE 487
            N+    L++
Sbjct: 360 ENSFDLGLID 350

BLAST of Chy4G074190 vs. ExPASy TrEMBL
Match: A0A5D3CA69 (Oxoglutarate/iron-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold606G001030 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.4e-271
Identity = 471/502 (93.82%), Postives = 488/502 (97.21%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGGPRYAGRGHPNNRGRSPRSAD+FTYRPRHAGGEG SVAGSSNPGSGAFRGRSSHQM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
           SSRNFRKPVGQKQASSSEQWQWRPLNS KDASPGAVDL LQHNSTDDMSNTNKQLLGSIA
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASPGAVDLHLQHNSTDDMSNTNKQLLGSIA 120

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
           SNSDSNQI NTSEQ+LG IASNSDCIEL SSSAQNVSKSLHSAVERIQIQGPTAVCG+CS
Sbjct: 121 SNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGNCS 180

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFP+DN N SDVVGQELKVQP+L+SCA+D+S TI+LGESNNVFNS DSKDKKPSVDLD+
Sbjct: 181 DSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDKKPSVDLDT 240

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDICPPKTGGV LNP LLAMNREKRNEM+RAM+GNNGIVLRPGMVHLKG ISLRDQAKIV
Sbjct: 241 FDICPPKTGGVTLNPSLLAMNREKRNEMKRAMDGNNGIVLRPGMVHLKGSISLRDQAKIV 300

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGD+RPFDDTKPPNLPDEF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLPDEF 360

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           YQLV+KAIKDSYAI+A+DSTIKNPERVLPWMKP+ICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           DKGLPVISFSIGDSAEFLFGD SDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA
Sbjct: 421 DKGLPVISFSIGDSAEFLFGDWSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480

Query: 481 PKALLEATNLRPGRLNLTFRQY 503
           PKALLEATNLRPGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 502

BLAST of Chy4G074190 vs. ExPASy TrEMBL
Match: A0A1S4E4H2 (LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 OS=Cucumis melo OX=3656 GN=LOC103501985 PE=4 SV=1)

HSP 1 Score: 939.5 bits (2427), Expect = 5.8e-270
Identity = 470/502 (93.63%), Postives = 487/502 (97.01%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGGPRYAGRGHPNNRGRSPRSAD+FTYRPRHAGGEG SVAGSSNPGSGAFRGRSSHQM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
           SSRNFRKPVGQKQASSSEQWQWRPLNS KDASPGAVDL LQHNSTDDMSNTNKQLLGSIA
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASPGAVDLHLQHNSTDDMSNTNKQLLGSIA 120

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
           SNSDSNQI NTSEQ+LG IASNSDCIEL SSSAQNVSKSLHSAVERIQIQGPTAVCG+CS
Sbjct: 121 SNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGNCS 180

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFP+DN N SDVVGQELKVQP+L+SCA+D+S TI+LGESNNVFNS DSKDKKPSVDLD+
Sbjct: 181 DSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDKKPSVDLDT 240

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDICPPKTGGV LNP LLAMNREK NEM+RAM+GNNGIVLRPGMVHLKG ISLRDQAKIV
Sbjct: 241 FDICPPKTGGVTLNPSLLAMNREK-NEMKRAMDGNNGIVLRPGMVHLKGSISLRDQAKIV 300

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGD+RPFDDTKPPNLPDEF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLPDEF 360

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           YQLV+KAIKDSYAI+A+DSTIKNPERVLPWMKP+ICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHS TA
Sbjct: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSKTA 480

Query: 481 PKALLEATNLRPGRLNLTFRQY 503
           PKALLEATNLRPGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 501

BLAST of Chy4G074190 vs. ExPASy TrEMBL
Match: A0A0A0LC72 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G651830 PE=4 SV=1)

HSP 1 Score: 934.5 bits (2414), Expect = 1.9e-268
Identity = 468/502 (93.23%), Postives = 475/502 (94.62%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEG SVAGSSNPGSGAFRGRSSHQM
Sbjct: 53  MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 112

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
           SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSN NKQL     
Sbjct: 113 SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNNNKQL----- 172

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
                          L SIASNSDCIELSSSSAQNVSKSLHSAVERI +QGPTAVCGS  
Sbjct: 173 ---------------LESIASNSDCIELSSSSAQNVSKSLHSAVERIHVQGPTAVCGSYG 232

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFP+DNCNRSDVVGQELKVQPSLKSCA+DESFTIQLG+SN+VFNSTDSKDKKPSVDLDS
Sbjct: 233 DSFPYDNCNRSDVVGQELKVQPSLKSCAKDESFTIQLGKSNDVFNSTDSKDKKPSVDLDS 292

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDICPPKTGGVMLNP LLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGIS+RDQAKIV
Sbjct: 293 FDICPPKTGGVMLNPSLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISVRDQAKIV 352

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF
Sbjct: 353 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 412

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           YQLV+KAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 413 YQLVEKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 472

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA
Sbjct: 473 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 532

Query: 481 PKALLEATNLRPGRLNLTFRQY 503
           PKALLEATNLRPGRLNLTFRQY
Sbjct: 533 PKALLEATNLRPGRLNLTFRQY 534

BLAST of Chy4G074190 vs. ExPASy TrEMBL
Match: A0A6J1IWW9 (uncharacterized protein LOC111479925 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479925 PE=4 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.7e-219
Identity = 395/502 (78.69%), Postives = 429/502 (85.46%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGG RY GRGHPNNRGR PRSAD FTYRPRHAGGEG SVAGSSNP +GAF+ RSSH  
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADSFTYRPRHAGGEGPSVAGSSNPRNGAFKDRSSH-- 60

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
            SRN R   GQKQA  SEQWQWRPLNSGKD+SPGAVDLQ  HN+ DD++   KQL+GSI+
Sbjct: 61  PSRNSRNTFGQKQALGSEQWQWRPLNSGKDSSPGAVDLQQYHNTADDIA--PKQLIGSIS 120

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
           SNSD N + NTSEQ LGSIAS SD  E S SSAQNVSKSLHSAVERIQI+ PTA    C 
Sbjct: 121 SNSDGNHMSNTSEQLLGSIASKSDGNEHSPSSAQNVSKSLHSAVERIQIREPTAEGERCD 180

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFP+ +C RSD  GQE  VQ        D++ TI+L ESNNV +  DSKDKKPS +L+ 
Sbjct: 181 DSFPYHSCKRSDAAGQEPMVQ--------DQTATIKLRESNNVSDCKDSKDKKPSGNLEI 240

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDICPPK+G V LNP LL+ NREKRNEM+RAMEGNNG VLRPGMVHLK GISL DQ KIV
Sbjct: 241 FDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKIV 300

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           K+CRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGD+RPFD+T PPN+P EF
Sbjct: 301 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDNTTPPNMPVEF 360

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           Y+LV+KAIKDSYA++ +DS  KNPERVLPWMKP+ICIVNFY QNGRLGLHQDRDESQESL
Sbjct: 361 YKLVEKAIKDSYAVLGKDSNTKNPERVLPWMKPNICIVNFYLQNGRLGLHQDRDESQESL 420

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           +KGLPV+SFSIGDSAEFLFGDRSD+DQAEKVTLESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 EKGLPVVSFSIGDSAEFLFGDRSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNTA 480

Query: 481 PKALLEATNLRPGRLNLTFRQY 503
           PKALLEATNLRPGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 490

BLAST of Chy4G074190 vs. ExPASy TrEMBL
Match: A0A6J1GV59 (uncharacterized protein LOC111457830 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457830 PE=4 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.7e-219
Identity = 396/502 (78.88%), Postives = 428/502 (85.26%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGG RY GRGHPNNRGR PRSAD+FTYRPRHAGGEG S AGSSNP +GAF+ RSSH  
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADNFTYRPRHAGGEGPSAAGSSNPRNGAFKDRSSH-- 60

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
            SRN R   GQKQA  SEQWQWRPLNSGKDASPGAVDLQ  HN+ DD++   KQL+GSI+
Sbjct: 61  PSRNSRNTFGQKQALGSEQWQWRPLNSGKDASPGAVDLQQDHNTADDIA--PKQLIGSIS 120

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
           SNSD N + NTSEQ LGSIAS SD  E S  SAQNVSKSLHSAVERIQI+ PTA    C 
Sbjct: 121 SNSDGNDMSNTSEQLLGSIASKSDGNEHSPCSAQNVSKSLHSAVERIQIREPTAEGERCD 180

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFP+D+C RSD  GQEL VQ        D++ TI+L ESNNV +  DSKDKKP  +L+ 
Sbjct: 181 DSFPYDSCKRSDAGGQELMVQ--------DQTATIKLRESNNVSDCKDSKDKKPLGNLEI 240

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDICPPK+G V LNP LL+ NREKRNEM+RAMEGNNG VLRPGMVHLK GISL DQ KIV
Sbjct: 241 FDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKIV 300

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS YGD+RPFD+T PPN+P EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSKYGDVRPFDNTTPPNMPVEF 360

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           Y+LV+KAIKDSYA+M +DS  KNPERVLPWMKP+ICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YKLVEKAIKDSYAVMGKDSNTKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           +KGLPV+SFSIGDSAEFLFGD SD+DQAEKVTLESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 EKGLPVVSFSIGDSAEFLFGDWSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNTA 480

Query: 481 PKALLEATNLRPGRLNLTFRQY 503
           PKALLEATNLRPGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 490

BLAST of Chy4G074190 vs. NCBI nr
Match: KAA0058530.1 (Oxoglutarate/iron-dependent dioxygenase [Cucumis melo var. makuwa] >TYK07216.1 Oxoglutarate/iron-dependent dioxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 939 bits (2426), Expect = 0.0
Identity = 471/502 (93.82%), Postives = 488/502 (97.21%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGGPRYAGRGHPNNRGRSPRSAD+FTYRPRHAGGEG SVAGSSNPGSGAFRGRSSHQM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
           SSRNFRKPVGQKQASSSEQWQWRPLNS KDASPGAVDL LQHNSTDDMSNTNKQLLGSIA
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASPGAVDLHLQHNSTDDMSNTNKQLLGSIA 120

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
           SNSDSNQI NTSEQ+LG IASNSDCIEL SSSAQNVSKSLHSAVERIQIQGPTAVCG+CS
Sbjct: 121 SNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGNCS 180

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFP+DN N SDVVGQELKVQP+L+SCA+D+S TI+LGESNNVFNS DSKDKKPSVDLD+
Sbjct: 181 DSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDKKPSVDLDT 240

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDICPPKTGGV LNP LLAMNREKRNEM+RAM+GNNGIVLRPGMVHLKG ISLRDQAKIV
Sbjct: 241 FDICPPKTGGVTLNPSLLAMNREKRNEMKRAMDGNNGIVLRPGMVHLKGSISLRDQAKIV 300

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGD+RPFDDTKPPNLPDEF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLPDEF 360

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           YQLV+KAIKDSYAI+A+DSTIKNPERVLPWMKP+ICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           DKGLPVISFSIGDSAEFLFGD SDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA
Sbjct: 421 DKGLPVISFSIGDSAEFLFGDWSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480

Query: 481 PKALLEATNLRPGRLNLTFRQY 502
           PKALLEATNLRPGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 502

BLAST of Chy4G074190 vs. NCBI nr
Match: XP_016903133.1 (PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 [Cucumis melo])

HSP 1 Score: 933 bits (2411), Expect = 0.0
Identity = 470/502 (93.63%), Postives = 487/502 (97.01%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGGPRYAGRGHPNNRGRSPRSAD+FTYRPRHAGGEG SVAGSSNPGSGAFRGRSSHQM
Sbjct: 1   MNDGGPRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGSSVAGSSNPGSGAFRGRSSHQM 60

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
           SSRNFRKPVGQKQASSSEQWQWRPLNS KDASPGAVDL LQHNSTDDMSNTNKQLLGSIA
Sbjct: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSRKDASPGAVDLHLQHNSTDDMSNTNKQLLGSIA 120

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
           SNSDSNQI NTSEQ+LG IASNSDCIEL SSSAQNVSKSLHSAVERIQIQGPTAVCG+CS
Sbjct: 121 SNSDSNQISNTSEQRLGPIASNSDCIELLSSSAQNVSKSLHSAVERIQIQGPTAVCGNCS 180

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFP+DN N SDVVGQELKVQP+L+SCA+D+S TI+LGESNNVFNS DSKDKKPSVDLD+
Sbjct: 181 DSFPYDNRNGSDVVGQELKVQPTLESCAKDKSSTIKLGESNNVFNSMDSKDKKPSVDLDT 240

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDICPPKTGGV LNP LLAMNREK NEM+RAM+GNNGIVLRPGMVHLKG ISLRDQAKIV
Sbjct: 241 FDICPPKTGGVTLNPSLLAMNREK-NEMKRAMDGNNGIVLRPGMVHLKGSISLRDQAKIV 300

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGD+RPFDDTKPPNLPDEF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPNLPDEF 360

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           YQLV+KAIKDSYAI+A+DSTIKNPERVLPWMKP+ICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YQLVEKAIKDSYAIIAKDSTIKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHS TA
Sbjct: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSKTA 480

Query: 481 PKALLEATNLRPGRLNLTFRQY 502
           PKALLEATNLRPGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 501

BLAST of Chy4G074190 vs. NCBI nr
Match: XP_038875730.1 (uncharacterized protein LOC120068103 isoform X1 [Benincasa hispida])

HSP 1 Score: 838 bits (2165), Expect = 9.67e-304
Identity = 424/502 (84.46%), Postives = 453/502 (90.24%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGG RYAGRGHPNNRGRSPRSAD+FTYRPRHAGGEG SVAGSSNPG+G FR RSS  M
Sbjct: 1   MNDGGRRYAGRGHPNNRGRSPRSADNFTYRPRHAGGEGPSVAGSSNPGNGTFRDRSSQHM 60

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
           SSRN  KP GQKQASSSEQWQWRPLNSGKDAS  A+DLQ +HNS DD+SNT+KQLLGSIA
Sbjct: 61  SSRNVGKPFGQKQASSSEQWQWRPLNSGKDASSAAIDLQPEHNSADDLSNTSKQLLGSIA 120

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
           SN+DSN +  +S+Q LGSIASNS+C E S SSAQNVSKSLHSAVERIQI+  TAV  SCS
Sbjct: 121 SNTDSNHMSKSSKQLLGSIASNSNCKEFSPSSAQNVSKSLHSAVERIQIRESTAVGESCS 180

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFPHDNCNRSD VGQ+LKVQ  L+SC +DES T +L ESNNV  STDSKDKKPSV+LD 
Sbjct: 181 DSFPHDNCNRSDAVGQDLKVQVLLESCVKDESSTKKLRESNNVSGSTDSKDKKPSVNLDP 240

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDIC PKTG V LNP L A NREKRNEM+RAMEGN+GIVLRPGMVHLK GISLRDQ  IV
Sbjct: 241 FDICLPKTGVVTLNPSLFAKNREKRNEMKRAMEGNSGIVLRPGMVHLKSGISLRDQVMIV 300

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           K+CRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGD+RPFDDTKPP+LPDEF
Sbjct: 301 KRCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDVRPFDDTKPPSLPDEF 360

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           YQLV+KAIK SYAIM +DST+KNPERVLPWMKP+ICIVNFYSQNGRLGLHQDRDES+ESL
Sbjct: 361 YQLVEKAIKVSYAIMGKDSTMKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESRESL 420

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           DKGLPV+SFSIGDSAEFLFGD+SD DQAEKVTLESGDILIFGGKSRHVFHGVT IH NTA
Sbjct: 421 DKGLPVVSFSIGDSAEFLFGDQSDADQAEKVTLESGDILIFGGKSRHVFHGVTVIHPNTA 480

Query: 481 PKALLEATNLRPGRLNLTFRQY 502
           PK LLEATNLRPGRLNLTFRQY
Sbjct: 481 PKELLEATNLRPGRLNLTFRQY 502

BLAST of Chy4G074190 vs. NCBI nr
Match: KAG6581809.1 (hypothetical protein SDJN03_21811, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 767 bits (1981), Expect = 6.36e-276
Identity = 397/502 (79.08%), Postives = 430/502 (85.66%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGG RY GRGHPNNRGR PRSAD+FTYRPRHAGGEG S AGSSNP +GAF+ RSSH  
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADNFTYRPRHAGGEGPSAAGSSNPRNGAFKDRSSHP- 60

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
            SRN R   GQKQA  SEQWQWRPLNSGKDAS GAVDLQ  HN+ DD++   KQL+GSI+
Sbjct: 61  -SRNSRNTFGQKQALGSEQWQWRPLNSGKDASAGAVDLQQDHNTADDIAP--KQLIGSIS 120

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
           SNSD N + NTSEQ LGSIAS SD  E S SSAQNVSKSLHSAVERIQI+ PTA    C+
Sbjct: 121 SNSDGNHMSNTSEQLLGSIASKSDGNEHSPSSAQNVSKSLHSAVERIQIREPTAEGERCN 180

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFP+D+C RSD  GQEL VQ        D++ TI+L ESNNV +  DSKDKKP  +L+ 
Sbjct: 181 DSFPYDSCKRSDAGGQELMVQ--------DQTATIKLRESNNVSDCKDSKDKKPLGNLEI 240

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDICPPK+G V LNP LL+ NREKRNEM+RAMEGNNG VLRPGMVHLK GISL DQ KIV
Sbjct: 241 FDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKIV 300

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS YGD+RPFD+T PPN+P EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSKYGDVRPFDNTTPPNMPVEF 360

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           Y+LV+KAIKDSYA+M +DS  KNPERVLPWMKP+ICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YKLVEKAIKDSYAVMGKDSNTKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           +KGLPV+SFSIGDSAEFLFGDRSD+DQAEKVTLESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 EKGLPVVSFSIGDSAEFLFGDRSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNTA 480

Query: 481 PKALLEATNLRPGRLNLTFRQY 502
           PKALLEATNLRPGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 490

BLAST of Chy4G074190 vs. NCBI nr
Match: KAG7018258.1 (alkB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 767 bits (1980), Expect = 9.03e-276
Identity = 397/502 (79.08%), Postives = 429/502 (85.46%), Query Frame = 0

Query: 1   MNDGGPRYAGRGHPNNRGRSPRSADHFTYRPRHAGGEGFSVAGSSNPGSGAFRGRSSHQM 60
           MNDGG RY GRGHPNNRGR PRSAD+FTYRPRHAGGEG S AGSSNP +GAF+ RSSH  
Sbjct: 1   MNDGGHRYGGRGHPNNRGRFPRSADNFTYRPRHAGGEGPSAAGSSNPRNGAFKDRSSHP- 60

Query: 61  SSRNFRKPVGQKQASSSEQWQWRPLNSGKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIA 120
            SRN R   GQKQA  SEQWQWRPLNSGKDAS GAVDLQ  HN+ DD++   KQL+GSI+
Sbjct: 61  -SRNSRNTFGQKQALGSEQWQWRPLNSGKDASAGAVDLQQDHNTADDIAP--KQLIGSIS 120

Query: 121 SNSDSNQIRNTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCS 180
           SNSD N + NTSEQ LGSIAS SD  E S SSAQNVSKSLHSAVERIQI+ PTA    C 
Sbjct: 121 SNSDGNHMSNTSEQLLGSIASKSDGNEHSPSSAQNVSKSLHSAVERIQIREPTAEGERCD 180

Query: 181 DSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS 240
           DSFP+D+C RSD  GQEL VQ        D++ TI+L ESNNV +  DSKDKKP  +L+ 
Sbjct: 181 DSFPYDSCKRSDAGGQELMVQ--------DQTATIKLRESNNVSDCKDSKDKKPLGNLEI 240

Query: 241 FDICPPKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIV 300
           FDICPPK+G V LNP LL+ NREKRNEM+RAMEGNNG VLRPGMVHLK GISL DQ KIV
Sbjct: 241 FDICPPKSGVVTLNPSLLSKNREKRNEMKRAMEGNNGNVLRPGMVHLKSGISLSDQVKIV 300

Query: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEF 360
           KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSS YGD+RPFD+T PPN+P EF
Sbjct: 301 KKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSKYGDVRPFDNTTPPNMPVEF 360

Query: 361 YQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESL 420
           Y+LV+KAIKDSYA+M +DS  KNPERVLPWMKP+ICIVNFYSQNGRLGLHQDRDESQESL
Sbjct: 361 YKLVEKAIKDSYAVMGKDSNTKNPERVLPWMKPNICIVNFYSQNGRLGLHQDRDESQESL 420

Query: 421 DKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTA 480
           +KGLPV+SFSIGDSAEFLFGDRSD+DQAEKVTLESGDILIFGGKSRHVFHGVTAIH NTA
Sbjct: 421 EKGLPVVSFSIGDSAEFLFGDRSDIDQAEKVTLESGDILIFGGKSRHVFHGVTAIHQNTA 480

Query: 481 PKALLEATNLRPGRLNLTFRQY 502
           PKALLEATNLRPGRLNLTFRQY
Sbjct: 481 PKALLEATNLRPGRLNLTFRQY 490

BLAST of Chy4G074190 vs. TAIR 10
Match: AT3G14160.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 329.7 bits (844), Expect = 4.1e-90
Identity = 195/437 (44.62%), Postives = 257/437 (58.81%), Query Frame = 0

Query: 73  QASSSEQWQWRPLNS---GKDASPGAVDLQLQHNSTDDMSNTNKQLLGSIASNSDSNQIR 132
           Q     +  W P NS   G D          +H+     +  NK  +G +       Q R
Sbjct: 47  QGKGRSRTSWSPRNSYGRGND----------EHSPV--QAYVNKSNVGFV--EKGVQQDR 106

Query: 133 NTSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVERIQIQGPTAVCGSCSD----SFPH 192
            + E  +GS     D      + A   SKS +       + G + V   C D        
Sbjct: 107 KSLEDGIGSTKQPDDGAAAGDNKAVLQSKSTN-------VSGGSFVSSECEDKDGAKMYC 166

Query: 193 DNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDSFDICP 252
           D  NR + V   L  Q S+ S    +     + +  +   +  + +         FDI  
Sbjct: 167 DLVNRVNDV--TLSCQESVSSTVVQKVELSSVEDQKSAPKADGAGNSSNESSTRHFDIFL 226

Query: 253 PKTGGVMLNPPLLAMNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIVKKCRD 312
            K  G++L P LL ++REK    ++A +G +G V+RPGMV LK  +S+ DQ  IV KCR 
Sbjct: 227 EKK-GIVLKPNLLVLSREK----KKAAKGYSGTVIRPGMVLLKNYLSINDQVMIVNKCRR 286

Query: 313 LGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEFYQLVQ 372
           LG+G GGFYQPGYR+  KLHLKMMCLGKNWDP++S YG+ RPFD +  P +P EF Q V+
Sbjct: 287 LGLGEGGFYQPGYRDEAKLHLKMMCLGKNWDPETSRYGETRPFDGSTAPRIPAEFNQFVE 346

Query: 373 KAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESLDKGLP 432
           KA+K+S ++ A +S        +P+M PDICIVNFYS  GRLGLHQD+DES+ S+ KGLP
Sbjct: 347 KAVKESQSLAASNSKQTKGGDEIPFMLPDICIVNFYSSTGRLGLHQDKDESENSIRKGLP 406

Query: 433 VISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTAPKALL 492
           V+SFSIGDSAEFL+GD+ D D+AE +TLESGD+L+FGG+SR VFHGV +I  +TAPKALL
Sbjct: 407 VVSFSIGDSAEFLYGDQRDEDKAETLTLESGDVLLFGGRSRKVFHGVRSIRKDTAPKALL 455

Query: 493 EATNLRPGRLNLTFRQY 503
           + T+LRPGRLNLTFRQY
Sbjct: 467 QETSLRPGRLNLTFRQY 455

BLAST of Chy4G074190 vs. TAIR 10
Match: AT5G01780.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 274.2 bits (700), Expect = 2.0e-73
Identity = 150/306 (49.02%), Postives = 201/306 (65.69%), Query Frame = 0

Query: 206 SCARDESFTIQLGESNNVFNS-TDSKDKKPS--VDLDSFDICPP--KTGGVMLNPPLLA- 265
           S    +S  +++ +  N  NS   S+D+ P    D   FDIC    +     +   +LA 
Sbjct: 92  SSKSSQSQNLKIRKVRNHRNSGFKSRDQSPQRIKDPPPFDICSSVLERNDTSIKDWILAD 151

Query: 266 -MNREKRNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIVKKCRDLGIGAGGFYQPGY 325
             NRE           N   V+RPGMV LK  ++   Q  IVK CR+LG+   GFYQPGY
Sbjct: 152 ETNRE------TVEVSNKHKVIRPGMVLLKDFLTPDIQVDIVKTCRELGVKPTGFYQPGY 211

Query: 326 REGGKLHLKMMCLGKNWDPDSS--TYGDIRPFDDTKPPNLPDEFYQLVQKAIKDSYAIMA 385
             G KLHL+MMCLG+NWDP +      DI    D+K P +P  F  LV+KAI++++A++ 
Sbjct: 212 SVGSKLHLQMMCLGRNWDPQTKYRKNTDI----DSKAPEIPVTFNVLVEKAIREAHALID 271

Query: 386 EDSTIKNPERVLPWMKPDICIVNFYSQNGRLGLHQDRDESQESLDKGLPVISFSIGDSAE 445
            +S  ++ ER+LP M PDICIVNFYS+ GRLGLHQDRDES+ES+ +GLP++SFSIGDSAE
Sbjct: 272 RESGTEDAERILPVMSPDICIVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAE 331

Query: 446 FLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTAPKALLEATNLRPGRLN 503
           FL+G++ DV++A+ V LESGD+LIFGG+SR +FHGV +I  N+AP +LL  + LR GRLN
Sbjct: 332 FLYGEKRDVEEAQGVILESGDVLIFGGESRMIFHGVKSIIPNSAPMSLLNESKLRTGRLN 387

BLAST of Chy4G074190 vs. TAIR 10
Match: AT5G01780.2 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 268.9 bits (686), Expect = 8.5e-72
Identity = 172/406 (42.36%), Postives = 237/406 (58.37%), Query Frame = 0

Query: 109 SNTNKQLLGSI--ASNSDSNQIRN-TSEQQLGSIASNSDCIELSSSSAQNVSKSLHSAVE 168
           S T K  LGS   ++   S+Q++N TS +   S+  N  C    +   +  S+ LH    
Sbjct: 64  SKTKKFYLGSTNPSTPCQSSQLQNWTSGKDALSLQRNLGC---KNRRRRRASRFLHEESN 123

Query: 169 RIQIQGPTAVCGSCSDSFPHDNCNRSDVVGQELKVQPSLKSCARDESFTIQLGESNNVFN 228
               +    + GS +     D+ N S              S    +S  +++ +  N  N
Sbjct: 124 GTTFEVGAGI-GSPTSMVHFDSTNPS-------------SSSKSSQSQNLKIRKVRNHRN 183

Query: 229 S-TDSKDKKPS--VDLDSFDICPP--KTGGVMLNPPLLA--MNREKRNEMRRAMEGNNGI 288
           S   S+D+ P    D   FDIC    +     +   +LA   NRE           N   
Sbjct: 184 SGFKSRDQSPQRIKDPPPFDICSSVLERNDTSIKDWILADETNRE------TVEVSNKHK 243

Query: 289 VLRPGMVHLKGGISLRDQAKIVKKCRDLGIGAGGFYQPGYREGGKLHLKMMCLGKNWDPD 348
           V+RPGMV LK  ++   Q  IVK CR+LG+   GFYQPGY  G KLHL+MMCLG+NWDP 
Sbjct: 244 VIRPGMVLLKDFLTPDIQVDIVKTCRELGVKPTGFYQPGYSVGSKLHLQMMCLGRNWDPQ 303

Query: 349 SS--TYGDIRPFDDTKPPNLPDEFYQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDIC 408
           +      DI    D+K P +P  F  LV+KAI++++A++  +S  ++ ER+LP M PDIC
Sbjct: 304 TKYRKNTDI----DSKAPEIPVTFNVLVEKAIREAHALIDRESGTEDAERILPVMSPDIC 363

Query: 409 IVNFYSQNGRLGLHQDRDESQESLDKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESG 468
           IVNFYS+ GRLGLHQDRDES+ES+ +GLP++SFSIGDSAEFL+G++ DV++A+ V LESG
Sbjct: 364 IVNFYSETGRLGLHQDRDESEESIARGLPIVSFSIGDSAEFLYGEKRDVEEAQGVILESG 423

Query: 469 DILIFGGKSRHVFHGVTAIHSNTAPKALLEATNLRPGRLNLTFRQY 503
           D+LIFGG+SR +FHGV +I  N+AP +LL  + LR GRLNLTFR +
Sbjct: 424 DVLIFGGESRMIFHGVKSIIPNSAPMSLLNESKLRTGRLNLTFRHF 442

BLAST of Chy4G074190 vs. TAIR 10
Match: AT3G14140.1 (2-oxoglutarate-dependent dioxygenase family protein )

HSP 1 Score: 268.5 bits (685), Expect = 1.1e-71
Identity = 140/299 (46.82%), Postives = 191/299 (63.88%), Query Frame = 0

Query: 206 SCARDESFTIQLGESNNVFNSTDSKDKKPSVDLDS-FDICPPKTGGVMLNPPLLAMNREK 265
           S   D++  +   E++ +    D      +    S FDI   K   + L P  L +NREK
Sbjct: 168 SSTSDKNVELSSVENHKIAPKADGPGNSSNESSSSPFDIFLKKK-VMRLKPSFLELNREK 227

Query: 266 RNEMRRAMEGNNGIVLRPGMVHLKGGISLRDQAKIVKKCRDLGIGAGGFYQPGYREGGKL 325
               ++A +G +GIV+RPGMV LK  +S+ +Q  IV KCR LG+G GGFYQPG+++GG L
Sbjct: 228 ----KKAAKGFSGIVIRPGMVLLKNYLSINNQVMIVNKCRQLGLGEGGFYQPGFQDGGLL 287

Query: 326 HLKMMCLGKNWDPDSSTYGDIRPFDDTKPPNLPDEFYQLVQKAIKDSYAIMAEDSTIKNP 385
           HLKMMCLGKNWD  +  YG+IRP D + PP +P EF QLV+KAIK+S +++A +S     
Sbjct: 288 HLKMMCLGKNWDCQTRRYGEIRPIDGSVPPRIPVEFSQLVEKAIKESKSLVATNSNETKG 347

Query: 386 ERVLPWMKPDICIVNFYSQNGRLGLHQ---------------------DRDESQESLDKG 445
              +P + PDIC+VNFY+  G+LGLHQ                     D+ ES++SL KG
Sbjct: 348 GDEIPLLLPDICVVNFYTSTGKLGLHQVSVYDKTSFDFLKYKGGYLNTDKGESKKSLRKG 407

Query: 446 LPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGKSRHVFHGVTAIHSNTAPK 483
           LP++SFSIGDSAEFL+GD+ DVD+A+ + LESGD+LIFG +SR+VFHGV +I     P+
Sbjct: 408 LPIVSFSIGDSAEFLYGDQKDVDKADTLILESGDVLIFGERSRNVFHGVRSIRKILPPR 461

BLAST of Chy4G074190 vs. TAIR 10
Match: AT1G11780.1 (oxidoreductase, 2OG-Fe(II) oxygenase family protein )

HSP 1 Score: 62.0 bits (149), Expect = 1.6e-09
Identity = 50/170 (29.41%), Postives = 74/170 (43.53%), Query Frame = 0

Query: 346 RPFDDTKP-PNLPDEFYQLVQKAIKDSYAIMAEDSTIKNPERVLPWMKPDICIVNFYSQN 405
           R +D + P  N+PD   QL     K   AI   D             +P+  IVN++   
Sbjct: 191 RNYDVSLPHNNIPDALCQLA----KTHAAIAMPDG---------EEFRPEGAIVNYFGIG 250

Query: 406 GRLGLHQDRDESQESLDKGLPVISFSIGDSAEFLFGDRSDVDQAEKVTLESGDILIFGGK 465
             LG H D  E+    D   P++S S+G  A FL G +S  D    + L SGD+++  G+
Sbjct: 251 DTLGGHLDDMEA----DWSKPIVSMSLGCKAIFLLGGKSKDDPPHAMYLRSGDVVLMAGE 310

Query: 466 SRHVFHGVTAIHS--NTAPKALLE-----------ATNLRPGRLNLTFRQ 502
           +R  FHG+  I +    A    LE           A  ++  R+N+  RQ
Sbjct: 311 ARECFHGIPRIFTGEENADIGALESELSHESGHFFAEYIKTSRININIRQ 343

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P050505.5e-1531.25Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Escherichia coli (strain K12) ... [more]
P374621.2e-1432.56Alpha-ketoglutarate-dependent dioxygenase AlkB OS=Salmonella typhimurium (strain... [more]
P0CAT76.0e-1440.87Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides... [more]
B8GWW66.0e-1440.87Alpha-ketoglutarate-dependent dioxygenase AlkB homolog OS=Caulobacter vibrioides... [more]
Q54N082.6e-0931.54Alpha-ketoglutarate-dependent dioxygenase alkB OS=Dictyostelium discoideum OX=44... [more]
Match NameE-valueIdentityDescription
A0A5D3CA691.4e-27193.82Oxoglutarate/iron-dependent dioxygenase OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S4E4H25.8e-27093.63LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 OS=Cucumis melo OX=365... [more]
A0A0A0LC721.9e-26893.23Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A6J1IWW92.7e-21978.69uncharacterized protein LOC111479925 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1GV592.7e-21978.88uncharacterized protein LOC111457830 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
KAA0058530.10.093.82Oxoglutarate/iron-dependent dioxygenase [Cucumis melo var. makuwa] >TYK07216.1 O... [more]
XP_016903133.10.093.63PREDICTED: LOW QUALITY PROTEIN: uncharacterized protein LOC103501985 [Cucumis me... [more]
XP_038875730.19.67e-30484.46uncharacterized protein LOC120068103 isoform X1 [Benincasa hispida][more]
KAG6581809.16.36e-27679.08hypothetical protein SDJN03_21811, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7018258.19.03e-27679.08alkB [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT3G14160.14.1e-9044.622-oxoglutarate-dependent dioxygenase family protein [more]
AT5G01780.12.0e-7349.022-oxoglutarate-dependent dioxygenase family protein [more]
AT5G01780.28.5e-7242.362-oxoglutarate-dependent dioxygenase family protein [more]
AT3G14140.11.1e-7146.822-oxoglutarate-dependent dioxygenase family protein [more]
AT1G11780.11.6e-0929.41oxidoreductase, 2OG-Fe(II) oxygenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR037151Alpha-ketoglutarate-dependent dioxygenase AlkB-like superfamilyGENE3D2.60.120.590coord: 278..502
e-value: 3.1E-52
score: 179.2
IPR027450Alpha-ketoglutarate-dependent dioxygenase AlkB-likePFAMPF135322OG-FeII_Oxy_2coord: 283..500
e-value: 7.1E-42
score: 143.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..95
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..86
NoneNo IPR availablePANTHERPTHR16557:SF92OG-FE(II) OXYGENASE FAMILY PROTEINcoord: 78..502
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 280..502
IPR004574Alkylated DNA repair protein AlkBPANTHERPTHR16557ALKYLATED DNA REPAIR PROTEIN ALKB-RELATEDcoord: 78..502
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 392..502
score: 10.799707

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G074190.1Chy4G074190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
molecular_function GO:0051213 dioxygenase activity