Chy4G070490 (gene) Cucumber (hystrix) v1

Overview
NameChy4G070490
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionCHY-type/CTCHY-type/RING-type Zinc finger protein
LocationchrH04: 2953836 .. 2956754 (-)
RNA-Seq ExpressionChy4G070490
SyntenyChy4G070490
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAACGAAGGAACCGGATGAATGCAAAATACCATGTGATGAAGAAAATTTACTGGCTTCCAGAGAAATAATAGATGGGTAAGTTGGGTTTTTGAAGGAAACTGAATGCGGAGATACAATTTGAGGTAGGCTTGTCCTTTTGGACAATTAAGCCAGTAGAACTATTAAATATTTTGCCCTTTTTTTTTTGTTATTTGTGGAACACATTTGATTGGGTGTGTTGCTTTTACCATAGGTTGATGCATCCACTGTTTGACTCAGTTTAGTGGGAATTGCATCCGGAGTCTTCAACAAATCAAGTGATCATGATGTGCAAATGGTCACTATTCAAAGCTGGAGGCTCTGGAGTCCCCCAGTGCTGCGTTGTAGTTATTATATGGGTTCCTATAACTCTTCTTTTTTGGATGAACTGAACTCTCCTCATGGAGTTCTGATGATTGGAGCTGTACAACGTTTCTTGGATAATATGTTGCTGATGTGCTGTGATCTGCAGCTGTATAGAAGAAAGCAACAGTGTCATGGCCTGTTTGACGGTGAATAACATGCCTTTTCTGGGTTTAGAATGATTGCTAAAGTCCTTCCTATATGCAGGTGCTTTAAGCTTCTATCCAGATTCAACCATTGTCCTGCATTGGAAGATATTATATTTCCATGGCAGAGGTTGCAACTTACTCAAAGCCTCAACCAATTGGAATGGAAGAAAATCAACACATTACATGCAGAGGAAAGGAGATAAGAACTGTGTACACTGAGCAGTTTGATTCTCCGGCGTTGCCCTTTGAGAAATCAAATGACCCCCAAAAGGTGTTGGAGAGGGAATACATGCAACATGGGTAAAGTCCTTGAAAGTTTTCTATTTCTTTCTAATCCTGTTTCAGGTCTTTCTTATTCATTTGCTAAACATGGGTCTTGTCATTTTGCAGATGTGCACATTATCGACGAAGGTGTCGTATCAGAGCCCCCTGTTGTAATGAAATATTTGATTGCCGTCATTGCCATAATGAAATAAAGGCAAGGAACTTCATTTTGAACCTGATTGTAGAATGTTTGTTGTTCTGAGCTATCATATACTGACTACAAAACCTTTATTTTAATTCTCGTACAGAATAGTATTTGTGTTGATCAGAAAGAAAGGCACGAGATACCACGCCACGAAGTCAACCAGGTAAATTTTCCAACTGGTTACTTGCCCAGCTTGTTTTAACCTCTTCACTTCTTCAGAAATTTCTCAGAGTTGAAGCATCTCTTCAAACTTACCTATAATTTTGTTCTCTCAGGTTATATGCTCACTCTGTGGAACTGAACAGGAGGTGTGTGCTTAATTTTACAATTTCAATTTTGGCTAAATGTTTAGTAATTTCGTGTTCATGAGCTCTACTTCACAGTTTTGTTCGTTTCTGATTTGGCAGGTTCGCCAAGACTGTATCAACTGTGGTGTGTGTTTTGGAAAATATTTCTGCAAGAGTTGCAAGCTGTTTGATGACGATGTGAGTTCTAGGCATCCACTTAGTTTTCCATTTTATCACCGATTCACCTCTTTTTTTTATGCACTAACAATTTAGTTAATTTAGTCACTTATTATATTTGTTGCCTATCTTGCAGACCTCTAAGAAGCAGTATCACTGTACTGGCTGCGGAATTTGTAGGTATGATCCATTCTAATCCATTTATCTTATTTTAAAACTATTTCTCTAATGTTTTCCATATGTTTACATGAAAACCTAATACTCGGTTGTATTGTTTGAGTTTTCGTTAGAGTTTCAACTCTTTTCTCACACTTAATGCCATTATAAACGTTTTGACAGAATTGGAGGGCGCGAAAACTTTTTCCACTGCCATAAGTGTGGTAAGTTATACGTAATTATCTGTTTCCTCTTTTGCTAAAATGTTTCACTTGTCGCAATTCATGTGCTGATTTTTATGTTTTCTTTGTCATGAAGGTTGCTGCTATTCAATTCTTCTAAAAAATAGCCATCCTTGCGTTGAAGGGGCAATGCACCATGACTGTCCTGTTTGCTTTGAGGTAAAGCTTTCCCCTCATTTACCTTAATTCATTTTCTATTTGTTCTCAGCAAGCACTAACTAATGATGTGCATCTTTCACAAATGGCTTGCAGTACCTATTCGATTCGACGAACGATGTTACGGTAATGCCATGTGGACACACCATCCATCAAAATTGCTTGAAGGAGATGAGAGATCACTTCCAGTAAGTCTTCATACTCAGTTCTTGTTTACTTATGGAACTTAGTTTAATGTGAAATGTATACACAAACTTTATTTTCTGATCACATGATTCTGCCACAGATATGCTTGCCCTCTTTGCTCCAAGTCCGTTTGTGATATGTCCAAGGTGTGGGAAAAAATTGACGTGGAAATTGCAGCTACGCCCATTCCAGAGCCATACCAAAACAAAATGGTAAACCTCCTTGCCCTCTTTTACTTGCATTTTCAATTTCATTTGCATGGCTAGGTCAATCATATATTATCACCTCTGGGGAGCATGTTTATTGGTAGTAGAATAAAAAATATTAAGTGCATGTTAAACAAATATTCTAATCCTAATTTTCACGTGGCAAATTTACTTTACTTTTGTTGAGAGTGGAATGCATAGAAACGAGTACAATTGTTCATATAATATCACAGTAGAAACTAGAAAATAGTGTTCAAATGGTCATCACTATGGTGTTTTTAAACCAAAATAATCTTCAAAGCATGCATGTGGGTATCTGGGTTGAGGTTAACAATGTACGACTATACACTCGTAAGCTTTTGATTCTTAAAAACTTAGTACTTTGAACGAACAGGTTTGGATTCTTTGCAACGATTGCGGTAAGACGTCAAAGGTGCAGTATCATGTTGTGGCTCAAAAGTGCTTGAATTGCAAGTCGTACAACACAAGACAGACCAGAGGCTAA

mRNA sequence

ATGGAAACGAAGGAACCGGATGAATGCAAAATACCATGTGATGAAGAAAATTTACTGGCTTCCAGAGAAATAATAGATGGTTTAGTGGGAATTGCATCCGGAGTCTTCAACAAATCAAGTGATCATGATGTGCAAATGGTCACTATTCAAAGCTGGAGGCTCTGGAGTCCCCCAGTGCTGCGTTGTAGTTATTATATGGGTTCCTATAACTCTTCTTTTTTGGATGAACTGAACTCTCCTCATGGAGTTCTGATGATTGGAGCTGTACAACGTTTCTTGGATAATATGTTGCTGATGTGCTGTGATCTGCAGCTGTATAGAAGAAAGCAACAGTGTCATGGCCTGTTTGACGAGGTTGCAACTTACTCAAAGCCTCAACCAATTGGAATGGAAGAAAATCAACACATTACATGCAGAGGAAAGGAGATAAGAACTGTGTACACTGAGCAGTTTGATTCTCCGGCGTTGCCCTTTGAGAAATCAAATGACCCCCAAAAGGTGTTGGAGAGGGAATACATGCAACATGGATGTGCACATTATCGACGAAGGTGTCGTATCAGAGCCCCCTGTTGTAATGAAATATTTGATTGCCGTCATTGCCATAATGAAATAAAGAATAGTATTTGTGTTGATCAGAAAGAAAGGCACGAGATACCACGCCACGAAGTCAACCAGGTTATATGCTCACTCTGTGGAACTGAACAGGAGGTTCGCCAAGACTGTATCAACTGTGGTGTGTGTTTTGGAAAATATTTCTGCAAGAGTTGCAAGCTGTTTGATGACGATACCTCTAAGAAGCAGTATCACTGTACTGGCTGCGGAATTTGTAGAATTGGAGGGCGCGAAAACTTTTTCCACTGCCATAAGTGTGGTTGCTGCTATTCAATTCTTCTAAAAAATAGCCATCCTTGCGTTGAAGGGGCAATGCACCATGACTGTCCTGTTTGCTTTGAGTACCTATTCGATTCGACGAACGATGTTACGGTAATGCCATGTGGACACACCATCCATCAAAATTGCTTGAAGGAGATGAGAGATCACTTCCAATATGCTTGCCCTCTTTGCTCCAAGTCCGTTTGTGATATGTCCAAGGTGTGGGAAAAAATTGACGTGGAAATTGCAGCTACGCCCATTCCAGAGCCATACCAAAACAAAATGGTTTGGATTCTTTGCAACGATTGCGGTAAGACGTCAAAGGTGCAGTATCATGTTGTGGCTCAAAAGTGCTTGAATTGCAAGTCGTACAACACAAGACAGACCAGAGGCTAA

Coding sequence (CDS)

ATGGAAACGAAGGAACCGGATGAATGCAAAATACCATGTGATGAAGAAAATTTACTGGCTTCCAGAGAAATAATAGATGGTTTAGTGGGAATTGCATCCGGAGTCTTCAACAAATCAAGTGATCATGATGTGCAAATGGTCACTATTCAAAGCTGGAGGCTCTGGAGTCCCCCAGTGCTGCGTTGTAGTTATTATATGGGTTCCTATAACTCTTCTTTTTTGGATGAACTGAACTCTCCTCATGGAGTTCTGATGATTGGAGCTGTACAACGTTTCTTGGATAATATGTTGCTGATGTGCTGTGATCTGCAGCTGTATAGAAGAAAGCAACAGTGTCATGGCCTGTTTGACGAGGTTGCAACTTACTCAAAGCCTCAACCAATTGGAATGGAAGAAAATCAACACATTACATGCAGAGGAAAGGAGATAAGAACTGTGTACACTGAGCAGTTTGATTCTCCGGCGTTGCCCTTTGAGAAATCAAATGACCCCCAAAAGGTGTTGGAGAGGGAATACATGCAACATGGATGTGCACATTATCGACGAAGGTGTCGTATCAGAGCCCCCTGTTGTAATGAAATATTTGATTGCCGTCATTGCCATAATGAAATAAAGAATAGTATTTGTGTTGATCAGAAAGAAAGGCACGAGATACCACGCCACGAAGTCAACCAGGTTATATGCTCACTCTGTGGAACTGAACAGGAGGTTCGCCAAGACTGTATCAACTGTGGTGTGTGTTTTGGAAAATATTTCTGCAAGAGTTGCAAGCTGTTTGATGACGATACCTCTAAGAAGCAGTATCACTGTACTGGCTGCGGAATTTGTAGAATTGGAGGGCGCGAAAACTTTTTCCACTGCCATAAGTGTGGTTGCTGCTATTCAATTCTTCTAAAAAATAGCCATCCTTGCGTTGAAGGGGCAATGCACCATGACTGTCCTGTTTGCTTTGAGTACCTATTCGATTCGACGAACGATGTTACGGTAATGCCATGTGGACACACCATCCATCAAAATTGCTTGAAGGAGATGAGAGATCACTTCCAATATGCTTGCCCTCTTTGCTCCAAGTCCGTTTGTGATATGTCCAAGGTGTGGGAAAAAATTGACGTGGAAATTGCAGCTACGCCCATTCCAGAGCCATACCAAAACAAAATGGTTTGGATTCTTTGCAACGATTGCGGTAAGACGTCAAAGGTGCAGTATCATGTTGTGGCTCAAAAGTGCTTGAATTGCAAGTCGTACAACACAAGACAGACCAGAGGCTAA

Protein sequence

METKEPDECKIPCDEENLLASREIIDGLVGIASGVFNKSSDHDVQMVTIQSWRLWSPPVLRCSYYMGSYNSSFLDELNSPHGVLMIGAVQRFLDNMLLMCCDLQLYRRKQQCHGLFDEVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNTRQTRG*
Homology
BLAST of Chy4G070490 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 3.8e-126
Identity = 196/257 (76.26%), Postives = 221/257 (85.99%), Query Frame = 0

Query: 163 DPQKVLEREYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHE 222
           D  + LE   MQ+GCAHYRRRCRIRAPCCNEIFDCRHCHNE KNSI +D  +RHE+PRHE
Sbjct: 47  DDYEKLEEGIMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDAVKRHELPRHE 106

Query: 223 VNQVICSLCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRE 282
           V QVICSLCGTEQEVRQ CI+CGVC GKYFC+ CKLFDDD SK+QYHC GCGICRIGG+E
Sbjct: 107 VQQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCGICRIGGKE 166

Query: 283 NFFHCHKCGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLK 342
           NFFHC KCGCCYSI+LKNSH CVEGAMHHDCP+CFEYLF+STNDV+V+PCGHTIH  CL+
Sbjct: 167 NFFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGHTIHVKCLR 226

Query: 343 EMRDHFQYACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQY 402
           EM +H Q+ACPLCSKSVCDMSK WE++D E+A   I +   NKMV ILCNDCG TS+VQ+
Sbjct: 227 EMEEHCQFACPLCSKSVCDMSKAWERLDEELAT--ISDTCDNKMVRILCNDCGATSEVQF 286

Query: 403 HVVAQKCLNCKSYNTRQ 420
           H++A KC  CKSYNTRQ
Sbjct: 287 HLIAHKCQKCKSYNTRQ 301

BLAST of Chy4G070490 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.1e-114
Identity = 169/263 (64.26%), Postives = 215/263 (81.75%), Query Frame = 0

Query: 159 EKSNDPQKVLEREYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEI 218
           E++ +   ++E     +GC+HYRRRC+IRAPCC+EIFDCRHCHNE K+S+ ++Q  RHE+
Sbjct: 9   EQNQEFANLMEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHEL 68

Query: 219 PRHEVNQVICSLCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRI 278
           PRHEV++VICSLC TEQ+V+Q+C NCGVC GKYFC  CK FDDD SKKQYHC  CGICR 
Sbjct: 69  PRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRT 128

Query: 279 GGRENFFHCHKCGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQ 338
           GG ENFFHC +C CCYS ++++ H CVEGAMHH+CPVCFEYLFDST D+TV+ CGHT+H 
Sbjct: 129 GGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHL 188

Query: 339 NCLKEMRDHFQYACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTS 398
            C K+M  H +Y CP+CSKS+CDMS +W+K+D E+AA P+P+ Y+NKMVWILCNDCG  +
Sbjct: 189 ECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGSNT 248

Query: 399 KVQYHVVAQKCLNCKSYNTRQTR 422
            V++H++A KC +C SYNTRQT+
Sbjct: 249 NVRFHLIAHKCSSCGSYNTRQTQ 271

BLAST of Chy4G070490 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 4.5e-95
Identity = 144/246 (58.54%), Postives = 183/246 (74.39%), Query Frame = 0

Query: 173 MQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCG 232
           M  GC HY+RRC+IRAPCCNE+FDCRHCHNE   S   +  +RH++ R +V QVICS+C 
Sbjct: 14  MGFGCKHYKRRCQIRAPCCNEVFDCRHCHNE-STSTLRNIYDRHDLVRQDVKQVICSVCD 73

Query: 233 TEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGC 292
           TEQ   Q C NCGV  G+YFC  C  +DDDT K+Q+HC  CGICR+GGRENFFHC KCG 
Sbjct: 74  TEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQQFHCDDCGICRVGGRENFFHCKKCGS 133

Query: 293 CYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYAC 352
           CY++ L+N+H CVE +M H CP+C+EYLFDS  D  VM CGHT+H  C  EM    ++ C
Sbjct: 134 CYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKDTNVMKCGHTMHVECYNEMIKRDKFCC 193

Query: 353 PLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNC 412
           P+CS+SV DMSK W+++D EI AT +P  Y++K VWILCNDC  T++V +H++ QKC +C
Sbjct: 194 PICSRSVIDMSKTWQRLDEEIEATAMPSDYRDKKVWILCNDCNDTTEVHFHIIGQKCGHC 253

Query: 413 KSYNTR 419
           +SYNTR
Sbjct: 254 RSYNTR 258

BLAST of Chy4G070490 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 3.9e-83
Identity = 128/245 (52.24%), Postives = 167/245 (68.16%), Query Frame = 0

Query: 173 MQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCG 232
           M  GC HYRRRCRIRAPCCN++F CRHCHNE         K+ HE+ RH V  VIC +C 
Sbjct: 14  MGFGCKHYRRRCRIRAPCCNDVFHCRHCHNE-------STKDGHELDRHAVESVICLVCD 73

Query: 233 TEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGC 292
           TEQ V Q C NCGVC G+YFC +CK FDDD  ++ +HC  CGICR+GG++NFFHC KCG 
Sbjct: 74  TEQPVAQVCYNCGVCMGEYFCSACKFFDDDVDREHFHCQDCGICRVGGKDNFFHCEKCGS 133

Query: 293 CYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYAC 352
           CYS+ L++ H C+E +M ++CP+C+EYLFDS  + +V+ CGHT+H  C  EM  H +++C
Sbjct: 134 CYSVSLRDKHCCIENSMKNNCPICYEYLFDSLRETSVLRCGHTMHLQCFHEMLKHDKFSC 193

Query: 353 PLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNC 412
           P+CS  + DM K    +D EI A  +   Y  K  WI+CNDC  T++V   V   KC +C
Sbjct: 194 PICSMPIFDMDKFLRALDAEIEANMLHIDYMGK-GWIVCNDCRDTTQVYARVAGHKCCHC 250

Query: 413 KSYNT 418
           +S+NT
Sbjct: 254 QSHNT 250

BLAST of Chy4G070490 vs. ExPASy Swiss-Prot
Match: Q96PM5 (RING finger and CHY zinc finger domain-containing protein 1 OS=Homo sapiens OX=9606 GN=RCHY1 PE=1 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 8.8e-67
Identity = 114/253 (45.06%), Postives = 151/253 (59.68%), Query Frame = 0

Query: 170 REYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICS 229
           +E  Q GC HY R C ++APCC++++ CR CH         D  E H++ R +V +V C 
Sbjct: 13  QERGQRGCEHYDRGCLLKAPCCDKLYTCRLCH---------DNNEDHQLDRFKVKEVQCI 72

Query: 230 LCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHK 289
            C   Q  +Q C  C   FG+Y+C  C LFD D  KKQYHC  CGICRIG +E+FFHC K
Sbjct: 73  NCEKIQHAQQTCEECSTLFGEYYCDICHLFDKD--KKQYHCENCGICRIGPKEDFFHCLK 132

Query: 290 CGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQ 349
           C  C ++ L+  H C+E     +CP+C E +  S     V+PCGH +H+ C +EM     
Sbjct: 133 CNLCLAMNLQGRHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKE-G 192

Query: 350 YACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKC 409
           Y CPLC  S  DM++ W ++D E+A TP+P  YQN  V ILCNDC   S VQ+H++  KC
Sbjct: 193 YRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKC 252

Query: 410 LNCKSYNTRQTRG 423
             C+SYNT Q  G
Sbjct: 253 KICESYNTAQAGG 253

BLAST of Chy4G070490 vs. ExPASy TrEMBL
Match: A0A0A0LHD3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G880030 PE=4 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.7e-190
Identity = 300/305 (98.36%), Postives = 303/305 (99.34%), Query Frame = 0

Query: 118 EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 177
           EVATYSKPQPIG EENQH+TCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC
Sbjct: 3   EVATYSKPQPIGTEENQHMTCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 62

Query: 178 AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 237
           AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV
Sbjct: 63  AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 122

Query: 238 RQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGCCYSIL 297
           RQDCINCGVCFGKYFC+SCKLFDDDTSKKQYHC GCGICRIGGRENFFHCHKCGCCYSIL
Sbjct: 123 RQDCINCGVCFGKYFCESCKLFDDDTSKKQYHCNGCGICRIGGRENFFHCHKCGCCYSIL 182

Query: 298 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 357
           LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK
Sbjct: 183 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 242

Query: 358 SVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT 417
           SVCDMSKVWEKID+EIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT
Sbjct: 243 SVCDMSKVWEKIDMEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT 302

Query: 418 RQTRG 423
           RQTRG
Sbjct: 303 RQTRG 307

BLAST of Chy4G070490 vs. ExPASy TrEMBL
Match: A0A1S3CSD5 (E3 ubiquitin-protein ligase MIEL1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503789 PE=4 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.6e-188
Identity = 297/305 (97.38%), Postives = 301/305 (98.69%), Query Frame = 0

Query: 118 EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 177
           EVATYSKPQPIG EENQH+TCRGKEIRTVYTEQFDS ALPFEKSNDPQ+VLEREYMQHGC
Sbjct: 3   EVATYSKPQPIGTEENQHMTCRGKEIRTVYTEQFDSLALPFEKSNDPQEVLEREYMQHGC 62

Query: 178 AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 237
           AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV
Sbjct: 63  AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 122

Query: 238 RQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGCCYSIL 297
           RQDCINCGVCFGKYFC+SCKLFDDDTSKKQYHC GCGICRIGGRENFFHCHKCGCCYSIL
Sbjct: 123 RQDCINCGVCFGKYFCESCKLFDDDTSKKQYHCNGCGICRIGGRENFFHCHKCGCCYSIL 182

Query: 298 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 357
           LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK
Sbjct: 183 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 242

Query: 358 SVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT 417
           SVCDMSKVWEKID+EIAATPIPEPYQNKMVWILCNDCG TSKVQYHVVAQKCLNCKSYNT
Sbjct: 243 SVCDMSKVWEKIDMEIAATPIPEPYQNKMVWILCNDCGTTSKVQYHVVAQKCLNCKSYNT 302

Query: 418 RQTRG 423
           RQTRG
Sbjct: 303 RQTRG 307

BLAST of Chy4G070490 vs. ExPASy TrEMBL
Match: A0A1S3CR85 (E3 ubiquitin-protein ligase MIEL1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103503789 PE=4 SV=1)

HSP 1 Score: 660.2 bits (1702), Expect = 5.7e-186
Identity = 297/313 (94.89%), Postives = 301/313 (96.17%), Query Frame = 0

Query: 118 EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 177
           EVATYSKPQPIG EENQH+TCRGKEIRTVYTEQFDS ALPFEKSNDPQ+VLEREYMQHGC
Sbjct: 3   EVATYSKPQPIGTEENQHMTCRGKEIRTVYTEQFDSLALPFEKSNDPQEVLEREYMQHGC 62

Query: 178 AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQE- 237
           AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQE 
Sbjct: 63  AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEF 122

Query: 238 -------VRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHK 297
                  VRQDCINCGVCFGKYFC+SCKLFDDDTSKKQYHC GCGICRIGGRENFFHCHK
Sbjct: 123 YWFLIWQVRQDCINCGVCFGKYFCESCKLFDDDTSKKQYHCNGCGICRIGGRENFFHCHK 182

Query: 298 CGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQ 357
           CGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQ
Sbjct: 183 CGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQ 242

Query: 358 YACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKC 417
           YACPLCSKSVCDMSKVWEKID+EIAATPIPEPYQNKMVWILCNDCG TSKVQYHVVAQKC
Sbjct: 243 YACPLCSKSVCDMSKVWEKIDMEIAATPIPEPYQNKMVWILCNDCGTTSKVQYHVVAQKC 302

Query: 418 LNCKSYNTRQTRG 423
           LNCKSYNTRQTRG
Sbjct: 303 LNCKSYNTRQTRG 315

BLAST of Chy4G070490 vs. ExPASy TrEMBL
Match: A0A6J1BQ41 (probable E3 ubiquitin-protein ligase RZFP34 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004617 PE=4 SV=1)

HSP 1 Score: 635.6 bits (1638), Expect = 1.5e-178
Identity = 278/305 (91.15%), Postives = 294/305 (96.39%), Query Frame = 0

Query: 118 EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 177
           EVATYSKPQ IG EENQH+TCRGKEIRT+YTEQ DS  L FEKSNDP+++LE  +MQ+GC
Sbjct: 3   EVATYSKPQQIGTEENQHMTCRGKEIRTMYTEQLDSQYLTFEKSNDPKELLEMGHMQYGC 62

Query: 178 AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 237
           AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRH+VNQVICSLCGTEQEV
Sbjct: 63  AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHDVNQVICSLCGTEQEV 122

Query: 238 RQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGCCYSIL 297
           RQ+CINCGVCFGKYFC++CKLFDDDTSKKQYHC GCGICRIGGRENFFHCHKCGCCYS +
Sbjct: 123 RQECINCGVCFGKYFCETCKLFDDDTSKKQYHCNGCGICRIGGRENFFHCHKCGCCYSNV 182

Query: 298 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 357
           +KNSHPC+EGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK
Sbjct: 183 IKNSHPCIEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 242

Query: 358 SVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT 417
           SVCDMSKVWEK D+EIAATPIPEPYQNKMVWILCNDCGKTSKVQYH+VAQKCLNCKSYNT
Sbjct: 243 SVCDMSKVWEKYDMEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHIVAQKCLNCKSYNT 302

Query: 418 RQTRG 423
           RQTRG
Sbjct: 303 RQTRG 307

BLAST of Chy4G070490 vs. ExPASy TrEMBL
Match: A0A6J1FBH5 (probable E3 ubiquitin-protein ligase RZFP34 OS=Cucurbita moschata OX=3662 GN=LOC111442596 PE=4 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 4.4e-170
Identity = 270/303 (89.11%), Postives = 283/303 (93.40%), Query Frame = 0

Query: 118 EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 177
           EVATYS+PQ IG E NQ +TCRGKE R   TEQFDS ALPFEKSNDPQ+++E  YMQ+GC
Sbjct: 3   EVATYSEPQQIGTEGNQQMTCRGKETR---TEQFDSQALPFEKSNDPQELMEMGYMQYGC 62

Query: 178 AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 237
           AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSI VDQKERHEIPRHEVNQVICSLCGTEQEV
Sbjct: 63  AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSISVDQKERHEIPRHEVNQVICSLCGTEQEV 122

Query: 238 RQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGCCYSIL 297
           RQ+CINCGVCFGKYFC +CKLFDDD SKKQYHC GCGICRIGGR NFFHC+KCGCCYSI+
Sbjct: 123 RQECINCGVCFGKYFCDACKLFDDDISKKQYHCNGCGICRIGGRHNFFHCYKCGCCYSII 182

Query: 298 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 357
           LKNSHPCVEGAMHHDCPVCFE+LFDSTNDVTVMPCGHTIH NCLKEMRDHFQ+ACPLCSK
Sbjct: 183 LKNSHPCVEGAMHHDCPVCFEFLFDSTNDVTVMPCGHTIHHNCLKEMRDHFQFACPLCSK 242

Query: 358 SVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT 417
           SVCDMSKVWEK D EIAATPIPEPYQNKMVWILCNDCGKTS VQYH+VAQKCLNCKSYNT
Sbjct: 243 SVCDMSKVWEKFDREIAATPIPEPYQNKMVWILCNDCGKTSNVQYHIVAQKCLNCKSYNT 302

Query: 418 RQT 421
           RQT
Sbjct: 303 RQT 302

BLAST of Chy4G070490 vs. NCBI nr
Match: KAG7024485.1 (putative E3 ubiquitin-protein ligase RZFP34, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 678 bits (1750), Expect = 1.56e-241
Identity = 331/452 (73.23%), Postives = 361/452 (79.87%), Query Frame = 0

Query: 1   METKEPDECKIPCDEENLLASREIIDGLVGIASGVFNKSSDHDVQMVTIQSWRLWSPP-- 60
           M T+EPD+CKIP DE   +      +    +      +SS +  Q++ I +W L+     
Sbjct: 86  MGTEEPDDCKIPYDEGKSIGFHRENNRWWELHP----ESSTN--QVIMICNWSLFKAGGS 145

Query: 61  -VLRCSYYM---GSYNSSFLDELNSPHGVLMIGAVQRFLDNMLLMCCDLQLYRRKQQCHG 120
            V +C   +    SYNSSFLDELNSP GV+MIGAVQR +D +LLMCC LQLYRRKQQCHG
Sbjct: 146 GVPQCCVVVIIWVSYNSSFLDELNSPLGVMMIGAVQRSMDYLLLMCCGLQLYRRKQQCHG 205

Query: 121 LFD-------------------EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPA 180
           LFD                   EVATYS+PQ IG E NQ +TCRGKE RT   EQFDS A
Sbjct: 206 LFDASTQIQPLSCIGGYYISMAEVATYSEPQQIGTEGNQQMTCRGKETRT---EQFDSQA 265

Query: 181 LPFEKSNDPQKVLEREYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIK-------NSI 240
           LPFEKSNDP +++E  YMQ+GCAHYRRRCRIRAPCCNEIFDCRHCHNEIK       NSI
Sbjct: 266 LPFEKSNDPHELMEMGYMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKARNYILSNSI 325

Query: 241 CVDQKERHEIPRHEVNQVICSLCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQY 300
            VDQKERHEIPRHEVNQVICSLCGTEQEVRQ+CINCGVCFGKYFC +CKLFDDD SKKQY
Sbjct: 326 SVDQKERHEIPRHEVNQVICSLCGTEQEVRQECINCGVCFGKYFCDACKLFDDDISKKQY 385

Query: 301 HCTGCGICRIGGRENFFHCHKCGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVT 360
           HC GCGICRIGGR+NFFHC+KCGCCYSI+LKNSHPCVEGAMHHDCPVCFE+LFDSTNDVT
Sbjct: 386 HCNGCGICRIGGRDNFFHCYKCGCCYSIILKNSHPCVEGAMHHDCPVCFEFLFDSTNDVT 445

Query: 361 VMPCGHTIHQNCLKEMRDHFQYACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVW 420
           VMPCGHTIH NCLKEMRDHFQ+ACPLCSKSVCDMSKVWEK D EIAATPIPEPYQNKMVW
Sbjct: 446 VMPCGHTIHHNCLKEMRDHFQFACPLCSKSVCDMSKVWEKFDREIAATPIPEPYQNKMVW 505

BLAST of Chy4G070490 vs. NCBI nr
Match: XP_004136332.1 (probable E3 ubiquitin-protein ligase RZFP34 [Cucumis sativus] >XP_031738775.1 probable E3 ubiquitin-protein ligase RZFP34 [Cucumis sativus] >KGN60137.1 hypothetical protein Csa_001208 [Cucumis sativus])

HSP 1 Score: 669 bits (1726), Expect = 1.99e-241
Identity = 300/305 (98.36%), Postives = 303/305 (99.34%), Query Frame = 0

Query: 118 EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 177
           EVATYSKPQPIG EENQH+TCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC
Sbjct: 3   EVATYSKPQPIGTEENQHMTCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 62

Query: 178 AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 237
           AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV
Sbjct: 63  AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 122

Query: 238 RQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGCCYSIL 297
           RQDCINCGVCFGKYFC+SCKLFDDDTSKKQYHC GCGICRIGGRENFFHCHKCGCCYSIL
Sbjct: 123 RQDCINCGVCFGKYFCESCKLFDDDTSKKQYHCNGCGICRIGGRENFFHCHKCGCCYSIL 182

Query: 298 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 357
           LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK
Sbjct: 183 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 242

Query: 358 SVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT 417
           SVCDMSKVWEKID+EIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT
Sbjct: 243 SVCDMSKVWEKIDMEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT 302

Query: 418 RQTRG 422
           RQTRG
Sbjct: 303 RQTRG 307

BLAST of Chy4G070490 vs. NCBI nr
Match: XP_008466355.1 (PREDICTED: E3 ubiquitin-protein ligase MIEL1 isoform X2 [Cucumis melo])

HSP 1 Score: 661 bits (1706), Expect = 2.22e-238
Identity = 297/305 (97.38%), Postives = 301/305 (98.69%), Query Frame = 0

Query: 118 EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 177
           EVATYSKPQPIG EENQH+TCRGKEIRTVYTEQFDS ALPFEKSNDPQ+VLEREYMQHGC
Sbjct: 3   EVATYSKPQPIGTEENQHMTCRGKEIRTVYTEQFDSLALPFEKSNDPQEVLEREYMQHGC 62

Query: 178 AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 237
           AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV
Sbjct: 63  AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 122

Query: 238 RQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGCCYSIL 297
           RQDCINCGVCFGKYFC+SCKLFDDDTSKKQYHC GCGICRIGGRENFFHCHKCGCCYSIL
Sbjct: 123 RQDCINCGVCFGKYFCESCKLFDDDTSKKQYHCNGCGICRIGGRENFFHCHKCGCCYSIL 182

Query: 298 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 357
           LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK
Sbjct: 183 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 242

Query: 358 SVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT 417
           SVCDMSKVWEKID+EIAATPIPEPYQNKMVWILCNDCG TSKVQYHVVAQKCLNCKSYNT
Sbjct: 243 SVCDMSKVWEKIDMEIAATPIPEPYQNKMVWILCNDCGTTSKVQYHVVAQKCLNCKSYNT 302

Query: 418 RQTRG 422
           RQTRG
Sbjct: 303 RQTRG 307

BLAST of Chy4G070490 vs. NCBI nr
Match: XP_008466354.1 (PREDICTED: E3 ubiquitin-protein ligase MIEL1 isoform X1 [Cucumis melo])

HSP 1 Score: 654 bits (1687), Expect = 2.35e-235
Identity = 297/313 (94.89%), Postives = 301/313 (96.17%), Query Frame = 0

Query: 118 EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 177
           EVATYSKPQPIG EENQH+TCRGKEIRTVYTEQFDS ALPFEKSNDPQ+VLEREYMQHGC
Sbjct: 3   EVATYSKPQPIGTEENQHMTCRGKEIRTVYTEQFDSLALPFEKSNDPQEVLEREYMQHGC 62

Query: 178 AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQE- 237
           AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQE 
Sbjct: 63  AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEF 122

Query: 238 -------VRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHK 297
                  VRQDCINCGVCFGKYFC+SCKLFDDDTSKKQYHC GCGICRIGGRENFFHCHK
Sbjct: 123 YWFLIWQVRQDCINCGVCFGKYFCESCKLFDDDTSKKQYHCNGCGICRIGGRENFFHCHK 182

Query: 298 CGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQ 357
           CGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQ
Sbjct: 183 CGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQ 242

Query: 358 YACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKC 417
           YACPLCSKSVCDMSKVWEKID+EIAATPIPEPYQNKMVWILCNDCG TSKVQYHVVAQKC
Sbjct: 243 YACPLCSKSVCDMSKVWEKIDMEIAATPIPEPYQNKMVWILCNDCGTTSKVQYHVVAQKC 302

Query: 418 LNCKSYNTRQTRG 422
           LNCKSYNTRQTRG
Sbjct: 303 LNCKSYNTRQTRG 315

BLAST of Chy4G070490 vs. NCBI nr
Match: XP_038898695.1 (probable E3 ubiquitin-protein ligase RZFP34 [Benincasa hispida] >XP_038898696.1 probable E3 ubiquitin-protein ligase RZFP34 [Benincasa hispida] >XP_038898697.1 probable E3 ubiquitin-protein ligase RZFP34 [Benincasa hispida])

HSP 1 Score: 648 bits (1672), Expect = 3.36e-233
Identity = 287/305 (94.10%), Postives = 299/305 (98.03%), Query Frame = 0

Query: 118 EVATYSKPQPIGMEENQHITCRGKEIRTVYTEQFDSPALPFEKSNDPQKVLEREYMQHGC 177
           EVATYSKPQ IG EENQH+TCRGKE+RTVYTEQFDS ALPFEKSN+PQ++LEREYM+HGC
Sbjct: 3   EVATYSKPQQIGTEENQHMTCRGKEMRTVYTEQFDSQALPFEKSNEPQELLEREYMEHGC 62

Query: 178 AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQVICSLCGTEQEV 237
           AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHE+PRHEVNQVICSLCGTEQEV
Sbjct: 63  AHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEMPRHEVNQVICSLCGTEQEV 122

Query: 238 RQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFFHCHKCGCCYSIL 297
           RQ+CINCGVCFGKYFC++CKLFDDDTSKKQYHC GCGICRIGGRENFFHCHKCGCCYS+L
Sbjct: 123 RQECINCGVCFGKYFCETCKLFDDDTSKKQYHCNGCGICRIGGRENFFHCHKCGCCYSVL 182

Query: 298 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 357
           LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK
Sbjct: 183 LKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMRDHFQYACPLCSK 242

Query: 358 SVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVVAQKCLNCKSYNT 417
           SVCDMSKVWEK D+EIAATPIPEPYQNKMVWILCNDC KTSKVQYHVVAQKCLNCKSYNT
Sbjct: 243 SVCDMSKVWEKFDMEIAATPIPEPYQNKMVWILCNDCSKTSKVQYHVVAQKCLNCKSYNT 302

Query: 418 RQTRG 422
           RQTRG
Sbjct: 303 RQTRG 307

BLAST of Chy4G070490 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 494.2 bits (1271), Expect = 1.0e-139
Identity = 213/257 (82.88%), Postives = 234/257 (91.05%), Query Frame = 0

Query: 166 KVLEREYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQ 225
           KVL+R  M++GC HYRRRC IRAPCCNEIF C HCH E KN+I VDQK+RH+IPRH+V Q
Sbjct: 52  KVLDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQ 111

Query: 226 VICSLCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFF 285
           VIC LCGTEQEV Q CI+CGVC GKYFCK CKL+DDDTSKKQYHC GCGICRIGGRENFF
Sbjct: 112 VICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFF 171

Query: 286 HCHKCGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMR 345
           HC+KCGCCYSILLKN HPCVEGAMHHDCP+CFE+LF+S NDVTV+PCGHTIHQ CL+EMR
Sbjct: 172 HCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMR 231

Query: 346 DHFQYACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVV 405
           DH+QYACPLCSKSVCDMSKVWEK D+EIAATP+PEPYQN+MV ILCNDCGK ++VQYHVV
Sbjct: 232 DHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKAEVQYHVV 291

Query: 406 AQKCLNCKSYNTRQTRG 423
           AQKC NCKSYNTRQTRG
Sbjct: 292 AQKCPNCKSYNTRQTRG 308

BLAST of Chy4G070490 vs. TAIR 10
Match: AT5G25560.3 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 486.5 bits (1251), Expect = 2.2e-137
Identity = 213/267 (79.78%), Postives = 235/267 (88.01%), Query Frame = 0

Query: 166 KVLEREYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEV-- 225
           KVL+R  M++GC HYRRRC IRAPCCNEIF C HCH E KN+I VDQK+RH+IPRH+V  
Sbjct: 52  KVLDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQ 111

Query: 226 --------NQVICSLCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGI 285
                   +QVIC LCGTEQEV Q CI+CGVC GKYFCK CKL+DDDTSKKQYHC GCGI
Sbjct: 112 LTRPLSSSSQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGI 171

Query: 286 CRIGGRENFFHCHKCGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHT 345
           CRIGGRENFFHC+KCGCCYSILLKN HPCVEGAMHHDCP+CFE+LF+S NDVTV+PCGHT
Sbjct: 172 CRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHT 231

Query: 346 IHQNCLKEMRDHFQYACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCG 405
           IHQ CL+EMRDH+QYACPLCSKSVCDMSKVWEK D+EIAATP+PEPYQN+MV ILCNDCG
Sbjct: 232 IHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCG 291

Query: 406 KTSKVQYHVVAQKCLNCKSYNTRQTRG 423
           K ++VQYHVVAQKC NCKSYNTRQTRG
Sbjct: 292 KKAEVQYHVVAQKCPNCKSYNTRQTRG 318

BLAST of Chy4G070490 vs. TAIR 10
Match: AT5G25560.4 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 477.6 bits (1228), Expect = 1.0e-134
Identity = 209/257 (81.32%), Postives = 230/257 (89.49%), Query Frame = 0

Query: 166 KVLEREYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQ 225
           KVL+R  M++G     RRC IRAPCCNEIF C HCH E KN+I VDQK+RH+IPRH+V Q
Sbjct: 52  KVLDRGLMEYG-----RRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQ 111

Query: 226 VICSLCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFF 285
           VIC LCGTEQEV Q CI+CGVC GKYFCK CKL+DDDTSKKQYHC GCGICRIGGRENFF
Sbjct: 112 VICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFF 171

Query: 286 HCHKCGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMR 345
           HC+KCGCCYSILLKN HPCVEGAMHHDCP+CFE+LF+S NDVTV+PCGHTIHQ CL+EMR
Sbjct: 172 HCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMR 231

Query: 346 DHFQYACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTSKVQYHVV 405
           DH+QYACPLCSKSVCDMSKVWEK D+EIAATP+PEPYQN+MV ILCNDCGK ++VQYHVV
Sbjct: 232 DHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCNDCGKKAEVQYHVV 291

Query: 406 AQKCLNCKSYNTRQTRG 423
           AQKC NCKSYNTRQTRG
Sbjct: 292 AQKCPNCKSYNTRQTRG 303

BLAST of Chy4G070490 vs. TAIR 10
Match: AT5G25560.2 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 428.7 bits (1101), Expect = 5.4e-120
Identity = 183/222 (82.43%), Postives = 202/222 (90.99%), Query Frame = 0

Query: 166 KVLEREYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEIPRHEVNQ 225
           KVL+R  M++GC HYRRRC IRAPCCNEIF C HCH E KN+I VDQK+RH+IPRH+V Q
Sbjct: 52  KVLDRGLMEYGCPHYRRRCCIRAPCCNEIFGCHHCHYEAKNNINVDQKQRHDIPRHQVEQ 111

Query: 226 VICSLCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRIGGRENFF 285
           VIC LCGTEQEV Q CI+CGVC GKYFCK CKL+DDDTSKKQYHC GCGICRIGGRENFF
Sbjct: 112 VICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDGCGICRIGGRENFF 171

Query: 286 HCHKCGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQNCLKEMR 345
           HC+KCGCCYSILLKN HPCVEGAMHHDCP+CFE+LF+S NDVTV+PCGHTIHQ CL+EMR
Sbjct: 172 HCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPCGHTIHQKCLEEMR 231

Query: 346 DHFQYACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMV 388
           DH+QYACPLCSKSVCDMSKVWEK D+EIAATP+PEPYQN+MV
Sbjct: 232 DHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMV 273

BLAST of Chy4G070490 vs. TAIR 10
Match: AT5G22920.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 414.8 bits (1065), Expect = 8.1e-116
Identity = 169/263 (64.26%), Postives = 215/263 (81.75%), Query Frame = 0

Query: 159 EKSNDPQKVLEREYMQHGCAHYRRRCRIRAPCCNEIFDCRHCHNEIKNSICVDQKERHEI 218
           E++ +   ++E     +GC+HYRRRC+IRAPCC+EIFDCRHCHNE K+S+ ++Q  RHE+
Sbjct: 9   EQNQEFANLMEIGSGHYGCSHYRRRCKIRAPCCDEIFDCRHCHNEAKDSLHIEQHHRHEL 68

Query: 219 PRHEVNQVICSLCGTEQEVRQDCINCGVCFGKYFCKSCKLFDDDTSKKQYHCTGCGICRI 278
           PRHEV++VICSLC TEQ+V+Q+C NCGVC GKYFC  CK FDDD SKKQYHC  CGICR 
Sbjct: 69  PRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECGICRT 128

Query: 279 GGRENFFHCHKCGCCYSILLKNSHPCVEGAMHHDCPVCFEYLFDSTNDVTVMPCGHTIHQ 338
           GG ENFFHC +C CCYS ++++ H CVEGAMHH+CPVCFEYLFDST D+TV+ CGHT+H 
Sbjct: 129 GGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGHTMHL 188

Query: 339 NCLKEMRDHFQYACPLCSKSVCDMSKVWEKIDVEIAATPIPEPYQNKMVWILCNDCGKTS 398
            C K+M  H +Y CP+CSKS+CDMS +W+K+D E+AA P+P+ Y+NKMVWILCNDCG  +
Sbjct: 189 ECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVAAYPMPKMYENKMVWILCNDCGSNT 248

Query: 399 KVQYHVVAQKCLNCKSYNTRQTR 422
            V++H++A KC +C SYNTRQT+
Sbjct: 249 NVRFHLIAHKCSSCGSYNTRQTQ 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5JL963.8e-12676.26Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3... [more]
Q9FFB61.1e-11464.26E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=... [more]
Q8VZK04.5e-9558.54E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ... [more]
Q10LI13.9e-8352.24E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR... [more]
Q96PM58.8e-6745.06RING finger and CHY zinc finger domain-containing protein 1 OS=Homo sapiens OX=9... [more]
Match NameE-valueIdentityDescription
A0A0A0LHD31.7e-19098.36Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G880030 PE=4 SV=1[more]
A0A1S3CSD53.6e-18897.38E3 ubiquitin-protein ligase MIEL1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A1S3CR855.7e-18694.89E3 ubiquitin-protein ligase MIEL1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC10350... [more]
A0A6J1BQ411.5e-17891.15probable E3 ubiquitin-protein ligase RZFP34 isoform X1 OS=Momordica charantia OX... [more]
A0A6J1FBH54.4e-17089.11probable E3 ubiquitin-protein ligase RZFP34 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
KAG7024485.11.56e-24173.23putative E3 ubiquitin-protein ligase RZFP34, partial [Cucurbita argyrosperma sub... [more]
XP_004136332.11.99e-24198.36probable E3 ubiquitin-protein ligase RZFP34 [Cucumis sativus] >XP_031738775.1 pr... [more]
XP_008466355.12.22e-23897.38PREDICTED: E3 ubiquitin-protein ligase MIEL1 isoform X2 [Cucumis melo][more]
XP_008466354.12.35e-23594.89PREDICTED: E3 ubiquitin-protein ligase MIEL1 isoform X1 [Cucumis melo][more]
XP_038898695.13.36e-23394.10probable E3 ubiquitin-protein ligase RZFP34 [Benincasa hispida] >XP_038898696.1 ... [more]
Match NameE-valueIdentityDescription
AT5G25560.11.0e-13982.88CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.32.2e-13779.78CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.41.0e-13481.32CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.25.4e-12082.43CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G22920.18.1e-11664.26CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 313..355
e-value: 6.1E-8
score: 42.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 312..355
e-value: 4.1E-8
score: 33.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 313..356
score: 12.1057
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 307..358
e-value: 1.1E-10
score: 42.8
IPR039512RCHY1, zinc-ribbonPFAMPF14599zinc_ribbon_6coord: 360..418
e-value: 5.7E-26
score: 90.3
NoneNo IPR availableGENE3D2.20.28.10coord: 384..422
e-value: 1.9E-18
score: 67.4
NoneNo IPR availablePANTHERPTHR21319RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1coord: 152..422
NoneNo IPR availablePANTHERPTHR21319:SF53CHY-TYPE/CTCHY-TYPE/RING-TYPE ZINC FINGER PROTEINcoord: 152..422
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 312..360
IPR008913Zinc finger, CHY-typePFAMPF05495zf-CHYcoord: 177..258
e-value: 3.8E-18
score: 65.8
IPR008913Zinc finger, CHY-typePROSITEPS51266ZF_CHYcoord: 170..246
score: 25.717133
IPR017921Zinc finger, CTCHY-typePROSITEPS51270ZF_CTCHYcoord: 248..312
score: 33.602699
IPR037275Zinc finger, CTCHY-type superfamilySUPERFAMILY161245Zinc hairpin stackcoord: 249..306
IPR037274Zinc finger, CHY-type superfamilySUPERFAMILY161219CHY zinc finger-likecoord: 174..248

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G070490.1Chy4G070490.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding