Chy4G068560 (gene) Cucumber (hystrix) v1

Overview
NameChy4G068560
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMethyltransf_11 domain-containing protein
LocationchrH04: 1243841 .. 1246802 (-)
RNA-Seq ExpressionChy4G068560
SyntenyChy4G068560
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATTGGCCGATGGTTCTGTATGTTTCGTTGTGAAAAATGTCGGCGGATTTGAATTTGATCCAGTTATACTTTTACTCAGAGTTTGAGTGATTCTTATTACGTCAGTTCCAAACTTCTTTCGGAAGGTATAGCCATCTGTGTAATGTTACAGTACCAGACTACCAGTAACCTTTTCAAGGTTTTATACATTTCATTTGTTTTTTCTGGTAGTGAAATAGAGTTCAAAGTTGTTGTTTCTATATTTTTCATTCCCGATTAAATTAGTCACATATGTGTGAAATTAGAGTGACAAAAATATATTGTGGAAAAAATGAATAGTTTACCAAATCTGTCGATTTGGCCTTTTCGAATTGCATTGTTTTTTTTCTCTCTAGATTTAATAATCTTCTGTTTTTTTATTTTGCATAGGGCTCTGTTATGATTGATAACGAAGACAATAAGATTAAGAATATCTTAGCTTGTTCCATATGTCATGGCCCATTGACAGCAGCAGCTGGTTCTGGCCTTTCAGTGTATGTTTGAAACTGTATTGTTGTACCTACTTTTGTTTTGTTTATTTCTTCTATTTCGTCTTAGTAATTGATGTGTCTTTATTTATATGTTTTGATACAGGGAGTCCACCAATGGATATCAATTGGAATGTGGCACATGTAAAAAGTCTTTCACTGGCAGTGAATCTCATTTGGACTTGGCGATAACCGGTGGAACTGACCGTGGTGAATCCATGCCTGCTGCCACTGAGATTTTTAGGTATTTCAACTTTATCGCCTGCTATTTATTGTGAACCGGTCGTTAATTAGCATGGTACTTAGACTTTCATATGTGAAGAGCTTCTCAATCTCAAGAGAATTTAATTCTGGTACAAATGCCCTGTAATGAATCTTTATGCTTTCTGTTGAAAAACAAACTAGAAACTGGGTATGCCATATACACAAAAAACCATTGGTACAATTCAATTGGCTGCAAATTCAGTATGGACATATTAGTTTACTTATCTGGGCTATTTTCATAAATATTTAGATTTTTTGCTATGTTGGAATTAGGACTTCACTGGTAGCTAGATAGCCTTAGTTGTATTAAGGAAACAGAACCTGTGTTTGGTTGGAACTTTTGATATCTTTCCCTCGCTGGCTCTCTCCCCTTTGTTTCTCATCTCATATTGTTCAGCTTTTCTCTGGATCAGGACTAGATTGGTTTCTTTTCTTTATGAGAGGGGCTGGCGTCAAAGTTTTTCAGTATTGCTTGGTTTTCCTGGCCCAGAAAAGGAGGTGATGTTAGCTCTTTTATTGCTCTTCATGATCAAAATGTCTACATGGTGCTTCTCATGTAATATCAGCAAGTATAGTTCAATTTTAGGAATTTGATCGGTCCATTAGTAATTCAATACTAATTCAAAGTTAGAGAGGGATTATTGTTTTGATCTAGAATGTTATATTACAGATGTTGGGGAGGGAATTCAAGCTTAACGTCCTATAAAGGGGAAGCCCTAGTGCCAACCCCCTGAGCCATGTTGAGGATGGCAAAGCTTATTGATTTTAATTACTATTTGCATGTCAACGAGACTTGTGATATCAATTCTAGATCTAGTTATTGTTCTCAATGCCTTGACATGATGATTTCATTGCAGTTCGAGTTGATAAAGAATTTCATAACACCAGTCTTAGGTGGAAGCATAATTGATGCAAGTTGTGGAAGTGGAATGTTTTCAAGAATCTTTGCCAAGAGTGGGTTATTTTCCTCTGTCGTTGCTTTGGACTACTCAGAGAACATGTTAAGGCAGTGCTATGATTTCATCAAACAAGAGGAAAACTTCCCCAACGAGTAAACAATATTCTCTTTTAGTTTTAGCTTTACTTGAGTAGCTGTGGAATTGCCATAGTTCAAGGATCTTCGTGTGGTATAATTTAAATTGTTGTTAAGTTTTTAATTTACATGGATCTTTTCCTTCTTAGGCGCTTGGTTCTGATCAGAGCTGATATTGCTCGACTTCCATTTGCTTCAAGTTCTGTTGATGTAGTGCATGCTGGTGCTGCTATACACTGCTGGCCTTCACCATCAGCTGCTGTGAGTAGTTTTCATCTATATCTGGGTTTATAGCATGTGATTTGACTTTCCATAGTTTGCACTAATTAAAATCACACGGCAGAAACTGCAGTTTGTTAATGGCTACTGGCTTTTCACTAGAGTGATATGCCTTCCCATCTTCAAAGGAAACCTATTTTAATACTCTTAACTTTGCTTCTCTGTTTGATTGTGAAATGTGTCTATCTGCTGGCACCTGCTGATGTATGTTGTGACATATACCTATAATTATACAATAGAATCATCCCACTAAAGTAATCCACGCCCCACGGACAAGCTTTTAGTAATTAACTGTGTGCATAATGAACTTTGTAGGTGGCTGAAATAAGTAGAATCTTGAGACCTGGAGGAGTATTTGTTGCCAGTACTTTCATAATGGATGGACCGTATTCTTTTGTACCATTTTTGAGGATTCAAATAGAGGTATGCTCGCAATTTGATTTCAATTTTCCTCTCTACAACTACTGGGATTCTCCTTCGAAGCTTGGGTAGTTTGATTACTTAAGTCCTATTATATCTTTTAATTTTTCACCCTGTCTGACTTCTGTTGCTTTTAAATTCTTTGATACTATCTTGAGTCCCAACTGCCAAGAAAAACAATCACATTTACTTAAGCTGGTTAAACCATATAAGTATGCTATCTTTAGGGACGGAGGATATGTCAATATCACTAAACCTTTCTCACTTTGGTCTAATTTAACCCTTAAGTTTTATTATTTGCATCACACTGCACAGGGTATTCAGCAGATATCAGGGAGCCGCATCTTCCTATCTGAACGAGAATTAGAAGAACTTTGCACAGCCTGCGGTTTAGTTGACTTTAAATGTTTAAGAAATAGGCAATTTGTAATGTTGTCCGCTACAAAACGCAGCTAA

mRNA sequence

ATGGAATTGGCCGATGGTTCTGGCTCTGTTATGATTGATAACGAAGACAATAAGATTAAGAATATCTTAGCTTGTTCCATATGTCATGGCCCATTGACAGCAGCAGCTGGTTCTGGCCTTTCAGTGGAGTCCACCAATGGATATCAATTGGAATGTGGCACATGTAAAAAGTCTTTCACTGGCAGTGAATCTCATTTGGACTTGGCGATAACCGGTGGAACTGACCGTGGTGAATCCATGCCTGCTGCCACTGAGATTTTTAGGACTAGATTGGTTTCTTTTCTTTATGAGAGGGGCTGGCGTCAAAGTTTTTCAGTATTGCTTGGTTTTCCTGGCCCAGAAAAGGAGTTCGAGTTGATAAAGAATTTCATAACACCAGTCTTAGGTGGAAGCATAATTGATGCAAGTTGTGGAAGTGGAATGTTTTCAAGAATCTTTGCCAAGAGTGGGTTATTTTCCTCTGTCGTTGCTTTGGACTACTCAGAGAACATGTTAAGGCAGTGCTATGATTTCATCAAACAAGAGGAAAACTTCCCCAACGAGCGCTTGGTTCTGATCAGAGCTGATATTGCTCGACTTCCATTTGCTTCAAGTTCTGTTGATGTAGTGCATGCTGGTGCTGCTATACACTGCTGGCCTTCACCATCAGCTGCTGTGGCTGAAATAAGTAGAATCTTGAGACCTGGAGGAGTATTTGTTGCCAGTACTTTCATAATGGATGGACCGTATTCTTTTGTACCATTTTTGAGGATTCAAATAGAGGGTATTCAGCAGATATCAGGGAGCCGCATCTTCCTATCTGAACGAGAATTAGAAGAACTTTGCACAGCCTGCGGTTTAGTTGACTTTAAATGTTTAAGAAATAGGCAATTTGTAATGTTGTCCGCTACAAAACGCAGCTAA

Coding sequence (CDS)

ATGGAATTGGCCGATGGTTCTGGCTCTGTTATGATTGATAACGAAGACAATAAGATTAAGAATATCTTAGCTTGTTCCATATGTCATGGCCCATTGACAGCAGCAGCTGGTTCTGGCCTTTCAGTGGAGTCCACCAATGGATATCAATTGGAATGTGGCACATGTAAAAAGTCTTTCACTGGCAGTGAATCTCATTTGGACTTGGCGATAACCGGTGGAACTGACCGTGGTGAATCCATGCCTGCTGCCACTGAGATTTTTAGGACTAGATTGGTTTCTTTTCTTTATGAGAGGGGCTGGCGTCAAAGTTTTTCAGTATTGCTTGGTTTTCCTGGCCCAGAAAAGGAGTTCGAGTTGATAAAGAATTTCATAACACCAGTCTTAGGTGGAAGCATAATTGATGCAAGTTGTGGAAGTGGAATGTTTTCAAGAATCTTTGCCAAGAGTGGGTTATTTTCCTCTGTCGTTGCTTTGGACTACTCAGAGAACATGTTAAGGCAGTGCTATGATTTCATCAAACAAGAGGAAAACTTCCCCAACGAGCGCTTGGTTCTGATCAGAGCTGATATTGCTCGACTTCCATTTGCTTCAAGTTCTGTTGATGTAGTGCATGCTGGTGCTGCTATACACTGCTGGCCTTCACCATCAGCTGCTGTGGCTGAAATAAGTAGAATCTTGAGACCTGGAGGAGTATTTGTTGCCAGTACTTTCATAATGGATGGACCGTATTCTTTTGTACCATTTTTGAGGATTCAAATAGAGGGTATTCAGCAGATATCAGGGAGCCGCATCTTCCTATCTGAACGAGAATTAGAAGAACTTTGCACAGCCTGCGGTTTAGTTGACTTTAAATGTTTAAGAAATAGGCAATTTGTAATGTTGTCCGCTACAAAACGCAGCTAA

Protein sequence

MELADGSGSVMIDNEDNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHLDLAITGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITPVLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLIRADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFVPFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS*
Homology
BLAST of Chy4G068560 vs. ExPASy Swiss-Prot
Match: Q8LBV4 (Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g78140 PE=1 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 2.2e-104
Identity = 187/284 (65.85%), Postives = 232/284 (81.69%), Query Frame = 0

Query: 20  KNILACSICHGPLT-AAAGSGLSVESTNGYQLECGTCKKSFTGSESHLDLAITGGTDR-G 79
           K ILAC IC+  L   +  +GL   + +G Q++C TCK+S++G+E+HLDLA+  G+ R  
Sbjct: 73  KKILACPICYNSLAWISQPNGLIESAASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYS 132

Query: 80  ESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITPVLGGSIIDASC 139
           E MP +TE+FRT LVSFLYERGWRQ+F +  GFPGPEKEFE+ K ++ PVLGG+IIDASC
Sbjct: 133 EPMPLSTELFRTPLVSFLYERGWRQNF-IWGGFPGPEKEFEMAKAYLKPVLGGNIIDASC 192

Query: 140 GSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPN-ERLVLIRADIARLPFA 199
           GSGMFSR+F +S LFS V+ALDYSENMLRQCY+ + +EENFPN E+LVL+RADIARLPF 
Sbjct: 193 GSGMFSRLFTRSDLFSLVIALDYSENMLRQCYELLNKEENFPNKEKLVLVRADIARLPFL 252

Query: 200 SSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFVPFLRIQIEGI 259
           S SVD VHAGAA+HCWPSPS+AVAEISR+LRPGGVFVA+TFI DGP+SF+PFL+   + I
Sbjct: 253 SGSVDAVHAGAALHCWPSPSSAVAEISRVLRPGGVFVATTFIYDGPFSFIPFLKNLRQEI 312

Query: 260 QQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 301
            + SGS IFL+ERELE++C ACGLV+F  +RN  F+MLSATK S
Sbjct: 313 MRYSGSHIFLNERELEDICKACGLVNFTRVRNGPFIMLSATKPS 355

BLAST of Chy4G068560 vs. ExPASy Swiss-Prot
Match: Q0WPT7 (Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g41040 PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 6.1e-70
Identity = 133/291 (45.70%), Postives = 192/291 (65.98%), Query Frame = 0

Query: 14  NEDNKIK----NILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHLDLA 73
           NE  KI+     + AC +C+ PL     SG+++++      +CG C K+++  + +LDL 
Sbjct: 66  NETPKIEIEETQVFACPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLT 125

Query: 74  ITGG-TDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITPVL 133
           +T    D  E  P  TE+FR+ LVSFLYERGWRQ+F    GFPGP++EF + + +     
Sbjct: 126 VTADLDDYNEVKPITTELFRSPLVSFLYERGWRQAFK-RSGFPGPDEEFRMAEEYFKEAE 185

Query: 134 GGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNE-RLVLIR 193
           GG ++D SCGSG+FSR FA+SG +S V+ALDYSENMLRQC +FIK +  F N   + ++R
Sbjct: 186 GGLLVDVSCGSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVR 245

Query: 194 ADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFVP 253
           AD++RLPF S SVD VHAGAA+HCWPSP+ A+AEI R+LR GGVFV +TF+   P +  P
Sbjct: 246 ADVSRLPFPSGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPST--P 305

Query: 254 FLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATK 299
           ++    +   +I  S  +L + E++++CT+CGL D++      F+M +A K
Sbjct: 306 WIIRPFQ--SRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARK 351

BLAST of Chy4G068560 vs. ExPASy Swiss-Prot
Match: Q6D3C1 (Malonyl-[acyl-carrier protein] O-methyltransferase OS=Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) OX=218491 GN=bioC PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 1.7e-08
Identity = 47/147 (31.97%), Postives = 72/147 (48.98%), Query Frame = 0

Query: 129 GGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLIRA 188
           G  ++DA CG+G FSR + ++G   +V ALD S  ML          E    +R   +  
Sbjct: 45  GLQVLDAGCGTGHFSRYWRQAG--RNVTALDLSAEMLAYA------REQHAADR--YLEG 104

Query: 189 DIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFVPF 248
           DI  LP A S VD+ ++  A+    S   A+ E+ RI RPGGV   +T + DG  S +  
Sbjct: 105 DIENLPLADSCVDICYSNLAVQWCDSLPRALGELYRITRPGGVIAFAT-LADGSLSELSQ 164

Query: 249 LRIQIEGIQQISGSRIFLSERELEELC 276
              +++G Q+   +  FL    ++  C
Sbjct: 165 AWQRLDGTQR---TNRFLPHSVIDAAC 177

BLAST of Chy4G068560 vs. ExPASy Swiss-Prot
Match: E3G327 (Malonyl-[acyl-carrier protein] O-methyltransferase OS=Enterobacter lignolyticus (strain SCF1) OX=701347 GN=bioC PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 8.6e-08
Identity = 45/141 (31.91%), Postives = 69/141 (48.94%), Query Frame = 0

Query: 132 IIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLIRADIA 191
           ++DA CG G  SR +  +G    V ALD S  ML +     ++++   +     ++ADI 
Sbjct: 46  VLDAGCGPGGISRYWRDNG--CEVTALDLSAQMLAEA----RRQQAADH----YVQADIE 105

Query: 192 RLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFVPFLRI 251
            +P AS+  D+V +  A+    S   AV E+ R+LRPGGV   +T   D     +P LR 
Sbjct: 106 AIPLASAQFDLVWSNLAVQWCDSLQDAVQELYRMLRPGGVLAFTTLAADS----LPELRQ 165

Query: 252 QIEGIQQISGSRIFLSERELE 273
               I +   +  FLS   L+
Sbjct: 166 AWRAIDEKPHANRFLSREALD 172

BLAST of Chy4G068560 vs. ExPASy Swiss-Prot
Match: Q2YY85 (Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain bovine RF122 / ET3-1) OX=273036 GN=menG PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.1e-07
Identity = 41/147 (27.89%), Postives = 67/147 (45.58%), Query Frame = 0

Query: 129 GGSIIDASCGSGMFSRIFAKS-GLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLIR 188
           G   +D  CG+G ++   +K+ G    V  +D+SENML      + +E+    E + L+ 
Sbjct: 50  GTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLE-----VGKEKTASMENVKLVH 109

Query: 189 ADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFVP 248
            D   LPF  +S D V  G  +   P    A+ E++R+L+PGG+ V          +F  
Sbjct: 110 GDAMELPFGDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLPAFKQ 169

Query: 249 FLRIQIEGIQQISGSRIFLSERELEEL 275
              +  + +  I G     S+ E E L
Sbjct: 170 MYALYFKFVMPIFGKLFAKSKEEYEWL 191

BLAST of Chy4G068560 vs. ExPASy TrEMBL
Match: A0A1S3CQA4 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503586 PE=4 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 8.3e-155
Identity = 279/294 (94.90%), Postives = 283/294 (96.26%), Query Frame = 0

Query: 7   SGSVMIDNEDNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHL 66
           S SVMID E NKIKNILACSIC GPLTAAA SGLSVESTNGYQLECGTCKKSFTGSESHL
Sbjct: 63  SDSVMIDKEVNKIKNILACSICRGPLTAAADSGLSVESTNGYQLECGTCKKSFTGSESHL 122

Query: 67  DLAITGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP 126
           DL ITGG + GESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP
Sbjct: 123 DLTITGGINSGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP 182

Query: 127 VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLI 186
           VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCY FIKQEENFPNERLVLI
Sbjct: 183 VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYGFIKQEENFPNERLVLI 242

Query: 187 RADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFV 246
           RADIARLPFASSSVD VHAGAA+HCWPSPSA VAEISRILRPGGVFVASTFIMDGP+SFV
Sbjct: 243 RADIARLPFASSSVDAVHAGAAVHCWPSPSAGVAEISRILRPGGVFVASTFIMDGPFSFV 302

Query: 247 PFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 301
           PFLRIQIEGIQQI+GSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS
Sbjct: 303 PFLRIQIEGIQQIAGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 356

BLAST of Chy4G068560 vs. ExPASy TrEMBL
Match: A0A6J1IKY5 (uncharacterized methyltransferase At1g78140, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111476562 PE=4 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.3e-136
Identity = 242/294 (82.31%), Postives = 265/294 (90.14%), Query Frame = 0

Query: 7   SGSVMIDNEDNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHL 66
           S  V+ID E N I+NILACS+C+GPLTAAAGSGLSVEST GYQLECGTCKKSFTGSESHL
Sbjct: 59  SDPVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHL 118

Query: 67  DLAITGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP 126
           DL I+ G +  ESMPA TEIFRTRLVSFLYERGWRQSFS +LGFPGPEKEFE IKNFITP
Sbjct: 119 DLTISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITP 178

Query: 127 VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLI 186
           VLGG+IIDASCGSGMFSRIFAKSGLFSSVVALDYSENML++CY FIK+EENFP E LVLI
Sbjct: 179 VLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLI 238

Query: 187 RADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFV 246
           RADIARLPFASSS+D VHAGAA+HCWPSPSAAVAEISRIL+PGGVFV ST+I+DGP+SF+
Sbjct: 239 RADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFM 298

Query: 247 PFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 301
           PFL  QIE  ++I G  I LSERELEELCTACG+VDF+CLRNR+FVM SA KRS
Sbjct: 299 PFLGTQIEIAKKIIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Chy4G068560 vs. ExPASy TrEMBL
Match: A0A6J1F9C0 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111443486 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 8.6e-136
Identity = 241/294 (81.97%), Postives = 264/294 (89.80%), Query Frame = 0

Query: 7   SGSVMIDNEDNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHL 66
           S  V+ID E N I+NILACS+C+GPLTAAAGSGLSVEST GYQLECGTCKKSFTGSESHL
Sbjct: 59  SDPVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHL 118

Query: 67  DLAITGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP 126
           DL I+ G +  ESMPA TEIFRTRLVSFLYERGWRQSFS +LGFPGPEKEFE IKNFITP
Sbjct: 119 DLTISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITP 178

Query: 127 VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLI 186
           VLGG+IIDASCGSGMFSRIFAKSGLFSSVVALDYSENML++CY FIK+EENFP E LVLI
Sbjct: 179 VLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLI 238

Query: 187 RADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFV 246
           RADIARLPFASSS+D VHAGAA+HCWPSPSAAVAEISRIL+PGGVFV ST+I+DGP+SF+
Sbjct: 239 RADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFM 298

Query: 247 PFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 301
           PFL  QIE  ++  G  I LSERELEELCTACG+VDF+CLRNR+FVM SA KRS
Sbjct: 299 PFLGTQIEIAKKTIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Chy4G068560 vs. ExPASy TrEMBL
Match: A0A6J1D0A7 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111016077 PE=4 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.7e-131
Identity = 236/296 (79.73%), Postives = 265/296 (89.53%), Query Frame = 0

Query: 7   SGSVMIDNEDNKIKNILACSICHGPLT-AAAGSGLSVESTNGYQLECGTCKKSFTGSESH 66
           S S ++D E N I+NILACS+C+GPLT AAAGSGLSVESTNGYQLECGTC+K+FTGS +H
Sbjct: 60  SDSSILDREVNNIENILACSVCYGPLTAAAAGSGLSVESTNGYQLECGTCRKTFTGSGTH 119

Query: 67  LDLAITGGTDR-GESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFI 126
           LDL I  G +  G+ MPA+TEIFRTRLVSFLYERGWRQSFSV LGFPGPE EFELI+ F+
Sbjct: 120 LDLTIASGNNNYGDPMPASTEIFRTRLVSFLYERGWRQSFSVALGFPGPETEFELIRTFM 179

Query: 127 TPVLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLV 186
           TP+LGG+IIDASCGSG+FSRIFAKSGLFSSVVALDYSENML+QCY+FI+QEENFP E LV
Sbjct: 180 TPILGGNIIDASCGSGLFSRIFAKSGLFSSVVALDYSENMLKQCYEFIQQEENFPRENLV 239

Query: 187 LIRADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYS 246
           LIRADIARLPFAS+SVD VHAGAA+HCWPSPSAAVAEISRILRPGGVFVASTFI DGP+S
Sbjct: 240 LIRADIARLPFASNSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIADGPFS 299

Query: 247 FVPFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 301
           F+PFL  Q E ++QI+GS I LSERELEELC ACGLV F+C+RNR FVM+SATK S
Sbjct: 300 FIPFLGTQRERVKQITGSHIVLSERELEELCRACGLVGFRCVRNRLFVMISATKPS 355

BLAST of Chy4G068560 vs. ExPASy TrEMBL
Match: A0A6P4AHU7 (uncharacterized methyltransferase At1g78140, chloroplastic OS=Ziziphus jujuba OX=326968 GN=LOC107420825 PE=4 SV=1)

HSP 1 Score: 419.5 bits (1077), Expect = 1.2e-113
Identity = 207/306 (67.65%), Postives = 254/306 (83.01%), Query Frame = 0

Query: 1   MELADGSGSVMIDNE-----DNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTC 60
           +E    +G V+++NE      +K KNILAC IC   L+  +   +SV+S +G  L+C TC
Sbjct: 51  VETKPPTGPVLVENEVSSSSSSKKKNILACPICFDLLSWRSDPVISVDSISGSSLQCSTC 110

Query: 61  KKSFTGSESHLDL-AITGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPE 120
           KK++ G+E+HLDL A +G  + GESMPA+TE+FRT LVSFLYERGWRQSFSV  GFPGPE
Sbjct: 111 KKTYFGNETHLDLTAASGAKNYGESMPASTELFRTPLVSFLYERGWRQSFSVWGGFPGPE 170

Query: 121 KEFELIKNFITPVLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQ 180
            EFEL K+F+ PVLGG+I+DASC SG+FSR+FAKSGLFS VVALDYSE+ML+QCY+FIKQ
Sbjct: 171 GEFELTKDFLKPVLGGNIVDASCASGLFSRLFAKSGLFSLVVALDYSESMLQQCYEFIKQ 230

Query: 181 EENFPNERLVLIRADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVA 240
           E+NFP E +VL+RADI+RLPF SSS+D VHAGAA+HCWPSPSAAVAEISR+LRPGGVFVA
Sbjct: 231 EDNFPKESIVLVRADISRLPFVSSSIDAVHAGAALHCWPSPSAAVAEISRVLRPGGVFVA 290

Query: 241 STFIMDGPYSFVPFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVML 300
           +T+I+DGP++FVPFLR   + I+QISGS+IFLSERELE+LC A GLV FKC+RNR FVM+
Sbjct: 291 TTYILDGPFAFVPFLRTARQNIRQISGSKIFLSERELEDLCRAGGLVGFKCVRNRAFVMI 350

BLAST of Chy4G068560 vs. NCBI nr
Match: XP_011652625.1 (uncharacterized methyltransferase At1g78140, chloroplastic [Cucumis sativus] >KAE8651419.1 hypothetical protein Csa_000773 [Cucumis sativus])

HSP 1 Score: 565 bits (1455), Expect = 1.68e-201
Identity = 286/296 (96.62%), Postives = 288/296 (97.30%), Query Frame = 0

Query: 7   SGSVMIDN--EDNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSES 66
           S SVMIDN  EDNKIKNILACSICHGPLTAAAGSGL VESTNGYQLECGTCKKSFTGSES
Sbjct: 58  SDSVMIDNDNEDNKIKNILACSICHGPLTAAAGSGLPVESTNGYQLECGTCKKSFTGSES 117

Query: 67  HLDLAITGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFI 126
           HLDL ITGGTD GESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFI
Sbjct: 118 HLDLTITGGTDSGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFI 177

Query: 127 TPVLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLV 186
           TPVLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCY+FIKQEENFPNERLV
Sbjct: 178 TPVLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYEFIKQEENFPNERLV 237

Query: 187 LIRADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYS 246
           LIRADIARLPFASSSVD VHAGAA+HCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYS
Sbjct: 238 LIRADIARLPFASSSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYS 297

Query: 247 FVPFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 300
           FVPFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATK S
Sbjct: 298 FVPFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKCS 353

BLAST of Chy4G068560 vs. NCBI nr
Match: XP_008466034.1 (PREDICTED: uncharacterized methyltransferase At1g78140, chloroplastic [Cucumis melo])

HSP 1 Score: 554 bits (1427), Expect = 3.45e-197
Identity = 279/294 (94.90%), Postives = 283/294 (96.26%), Query Frame = 0

Query: 7   SGSVMIDNEDNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHL 66
           S SVMID E NKIKNILACSIC GPLTAAA SGLSVESTNGYQLECGTCKKSFTGSESHL
Sbjct: 63  SDSVMIDKEVNKIKNILACSICRGPLTAAADSGLSVESTNGYQLECGTCKKSFTGSESHL 122

Query: 67  DLAITGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP 126
           DL ITGG + GESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP
Sbjct: 123 DLTITGGINSGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP 182

Query: 127 VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLI 186
           VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCY FIKQEENFPNERLVLI
Sbjct: 183 VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYGFIKQEENFPNERLVLI 242

Query: 187 RADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFV 246
           RADIARLPFASSSVD VHAGAA+HCWPSPSA VAEISRILRPGGVFVASTFIMDGP+SFV
Sbjct: 243 RADIARLPFASSSVDAVHAGAAVHCWPSPSAGVAEISRILRPGGVFVASTFIMDGPFSFV 302

Query: 247 PFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 300
           PFLRIQIEGIQQI+GSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS
Sbjct: 303 PFLRIQIEGIQQIAGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 356

BLAST of Chy4G068560 vs. NCBI nr
Match: XP_038896627.1 (uncharacterized methyltransferase At1g78140, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 515 bits (1327), Expect = 5.29e-183
Identity = 255/290 (87.93%), Postives = 270/290 (93.10%), Query Frame = 0

Query: 11  MIDNEDNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHLDLAI 70
           MID E N IKNILACSIC+GPLTAA  SGLSVESTNGYQLECGTCKKSFTGSESHLDL I
Sbjct: 1   MIDKEVNNIKNILACSICYGPLTAAVDSGLSVESTNGYQLECGTCKKSFTGSESHLDLTI 60

Query: 71  TGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITPVLGG 130
             G   GESMPAATEIFRTRLVSFLYERGWRQSFS  LGFPGPEKEFEL+KNF+TP+LGG
Sbjct: 61  ASGIKNGESMPAATEIFRTRLVSFLYERGWRQSFSPFLGFPGPEKEFELLKNFMTPILGG 120

Query: 131 SIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLIRADI 190
           +IIDASCGSGMFSRIFAKSGLFSSVVALDYSENML++CY+FIKQEENFP E LVLIRADI
Sbjct: 121 NIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYEFIKQEENFPKESLVLIRADI 180

Query: 191 ARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFVPFLR 250
           ARLPFASSSVD VHAGAA+HCWPSPSAAVAEISRILRPGGVFVASTFI+DGP+SF+PFL 
Sbjct: 181 ARLPFASSSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIIDGPFSFIPFLG 240

Query: 251 IQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 300
           IQIEGIQQI+GS I LSE EL+ELCTACGLVDFKCLRNR+FVMLSATKRS
Sbjct: 241 IQIEGIQQITGSHIVLSEGELKELCTACGLVDFKCLRNRRFVMLSATKRS 290

BLAST of Chy4G068560 vs. NCBI nr
Match: XP_038896626.1 (uncharacterized methyltransferase At1g78140, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 517 bits (1332), Expect = 9.76e-183
Identity = 257/294 (87.41%), Postives = 272/294 (92.52%), Query Frame = 0

Query: 7   SGSVMIDNEDNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHL 66
           S  VMID E N IKNILACSIC+GPLTAA  SGLSVESTNGYQLECGTCKKSFTGSESHL
Sbjct: 62  SDLVMIDKEVNNIKNILACSICYGPLTAAVDSGLSVESTNGYQLECGTCKKSFTGSESHL 121

Query: 67  DLAITGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP 126
           DL I  G   GESMPAATEIFRTRLVSFLYERGWRQSFS  LGFPGPEKEFEL+KNF+TP
Sbjct: 122 DLTIASGIKNGESMPAATEIFRTRLVSFLYERGWRQSFSPFLGFPGPEKEFELLKNFMTP 181

Query: 127 VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLI 186
           +LGG+IIDASCGSGMFSRIFAKSGLFSSVVALDYSENML++CY+FIKQEENFP E LVLI
Sbjct: 182 ILGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYEFIKQEENFPKESLVLI 241

Query: 187 RADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFV 246
           RADIARLPFASSSVD VHAGAA+HCWPSPSAAVAEISRILRPGGVFVASTFI+DGP+SF+
Sbjct: 242 RADIARLPFASSSVDAVHAGAALHCWPSPSAAVAEISRILRPGGVFVASTFIIDGPFSFI 301

Query: 247 PFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 300
           PFL IQIEGIQQI+GS I LSE EL+ELCTACGLVDFKCLRNR+FVMLSATKRS
Sbjct: 302 PFLGIQIEGIQQITGSHIVLSEGELKELCTACGLVDFKCLRNRRFVMLSATKRS 355

BLAST of Chy4G068560 vs. NCBI nr
Match: XP_022976042.1 (uncharacterized methyltransferase At1g78140, chloroplastic-like [Cucurbita maxima] >XP_022976043.1 uncharacterized methyltransferase At1g78140, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 493 bits (1268), Expect = 4.85e-173
Identity = 242/294 (82.31%), Postives = 265/294 (90.14%), Query Frame = 0

Query: 7   SGSVMIDNEDNKIKNILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHL 66
           S  V+ID E N I+NILACS+C+GPLTAAAGSGLSVEST GYQLECGTCKKSFTGSESHL
Sbjct: 59  SDPVLIDKEVNNIENILACSVCYGPLTAAAGSGLSVESTKGYQLECGTCKKSFTGSESHL 118

Query: 67  DLAITGGTDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITP 126
           DL I+ G +  ESMPA TEIFRTRLVSFLYERGWRQSFS +LGFPGPEKEFE IKNFITP
Sbjct: 119 DLTISAGINNEESMPATTEIFRTRLVSFLYERGWRQSFSAILGFPGPEKEFEFIKNFITP 178

Query: 127 VLGGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLI 186
           VLGG+IIDASCGSGMFSRIFAKSGLFSSVVALDYSENML++CY FIK+EENFP E LVLI
Sbjct: 179 VLGGNIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLKECYAFIKEEENFPKESLVLI 238

Query: 187 RADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFV 246
           RADIARLPFASSS+D VHAGAA+HCWPSPSAAVAEISRIL+PGGVFV ST+I+DGP+SF+
Sbjct: 239 RADIARLPFASSSIDAVHAGAALHCWPSPSAAVAEISRILKPGGVFVGSTYIIDGPFSFM 298

Query: 247 PFLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 300
           PFL  QIE  ++I G  I LSERELEELCTACG+VDF+CLRNR+FVM SA KRS
Sbjct: 299 PFLGTQIEIAKKIIGGHIVLSERELEELCTACGMVDFRCLRNRRFVMFSARKRS 352

BLAST of Chy4G068560 vs. TAIR 10
Match: AT1G78140.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 380.2 bits (975), Expect = 1.6e-105
Identity = 187/284 (65.85%), Postives = 232/284 (81.69%), Query Frame = 0

Query: 20  KNILACSICHGPLT-AAAGSGLSVESTNGYQLECGTCKKSFTGSESHLDLAITGGTDR-G 79
           K ILAC IC+  L   +  +GL   + +G Q++C TCK+S++G+E+HLDLA+  G+ R  
Sbjct: 73  KKILACPICYNSLAWISQPNGLIESAASGIQVQCNTCKRSYSGNETHLDLAVASGSKRYS 132

Query: 80  ESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITPVLGGSIIDASC 139
           E MP +TE+FRT LVSFLYERGWRQ+F +  GFPGPEKEFE+ K ++ PVLGG+IIDASC
Sbjct: 133 EPMPLSTELFRTPLVSFLYERGWRQNF-IWGGFPGPEKEFEMAKAYLKPVLGGNIIDASC 192

Query: 140 GSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPN-ERLVLIRADIARLPFA 199
           GSGMFSR+F +S LFS V+ALDYSENMLRQCY+ + +EENFPN E+LVL+RADIARLPF 
Sbjct: 193 GSGMFSRLFTRSDLFSLVIALDYSENMLRQCYELLNKEENFPNKEKLVLVRADIARLPFL 252

Query: 200 SSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFVPFLRIQIEGI 259
           S SVD VHAGAA+HCWPSPS+AVAEISR+LRPGGVFVA+TFI DGP+SF+PFL+   + I
Sbjct: 253 SGSVDAVHAGAALHCWPSPSSAVAEISRVLRPGGVFVATTFIYDGPFSFIPFLKNLRQEI 312

Query: 260 QQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATKRS 301
            + SGS IFL+ERELE++C ACGLV+F  +RN  F+MLSATK S
Sbjct: 313 MRYSGSHIFLNERELEDICKACGLVNFTRVRNGPFIMLSATKPS 355

BLAST of Chy4G068560 vs. TAIR 10
Match: AT2G41040.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 4.3e-71
Identity = 133/291 (45.70%), Postives = 192/291 (65.98%), Query Frame = 0

Query: 14  NEDNKIK----NILACSICHGPLTAAAGSGLSVESTNGYQLECGTCKKSFTGSESHLDLA 73
           NE  KI+     + AC +C+ PL     SG+++++      +CG C K+++  + +LDL 
Sbjct: 66  NETPKIEIEETQVFACPVCYEPLMRKGPSGINLQAIYRSGFKCGQCNKTYSSKDEYLDLT 125

Query: 74  ITGG-TDRGESMPAATEIFRTRLVSFLYERGWRQSFSVLLGFPGPEKEFELIKNFITPVL 133
           +T    D  E  P  TE+FR+ LVSFLYERGWRQ+F    GFPGP++EF + + +     
Sbjct: 126 VTADLDDYNEVKPITTELFRSPLVSFLYERGWRQAFK-RSGFPGPDEEFRMAEEYFKEAE 185

Query: 134 GGSIIDASCGSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNE-RLVLIR 193
           GG ++D SCGSG+FSR FA+SG +S V+ALDYSENMLRQC +FIK +  F N   + ++R
Sbjct: 186 GGLLVDVSCGSGLFSRKFAQSGKYSGVIALDYSENMLRQCKEFIKNDNTFDNSTNIAVVR 245

Query: 194 ADIARLPFASSSVDVVHAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIMDGPYSFVP 253
           AD++RLPF S SVD VHAGAA+HCWPSP+ A+AEI R+LR GGVFV +TF+   P +  P
Sbjct: 246 ADVSRLPFPSGSVDAVHAGAALHCWPSPTNAIAEICRVLRSGGVFVGTTFLRYSPST--P 305

Query: 254 FLRIQIEGIQQISGSRIFLSERELEELCTACGLVDFKCLRNRQFVMLSATK 299
           ++    +   +I  S  +L + E++++CT+CGL D++      F+M +A K
Sbjct: 306 WIIRPFQ--SRILQSYNYLMQDEIKDVCTSCGLTDYEDYIQDSFIMFTARK 351

BLAST of Chy4G068560 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 45.8 bits (107), Expect = 7.0e-05
Identity = 44/156 (28.21%), Postives = 66/156 (42.31%), Query Frame = 0

Query: 91  LVSFLYERGWRQSFSVLLGFPGPEKEFELIK---NFITPVL----GGSIIDASCGSGMFS 150
           L +  YE GW +SF     + G E   E IK   +F+   L    G  ++D  CG G   
Sbjct: 51  LATSFYEYGWGESFHFAQRWKG-ESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPL 110

Query: 151 RIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLIRADIARLPFASSSVDVV 210
           R  A+   FS+ V    + N  +             ++    ++AD  ++PF  +S D V
Sbjct: 111 REIAR---FSNSVVTGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAV 170

Query: 211 HAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIM 240
           +A  A    P       EI R+L+PG  F A  + M
Sbjct: 171 YAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCM 202

BLAST of Chy4G068560 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 45.8 bits (107), Expect = 7.0e-05
Identity = 44/156 (28.21%), Postives = 66/156 (42.31%), Query Frame = 0

Query: 91  LVSFLYERGWRQSFSVLLGFPGPEKEFELIK---NFITPVL----GGSIIDASCGSGMFS 150
           L +  YE GW +SF     + G E   E IK   +F+   L    G  ++D  CG G   
Sbjct: 51  LATSFYEYGWGESFHFAQRWKG-ESLRESIKRHEHFLALQLGIQPGQKVLDVGCGIGGPL 110

Query: 151 RIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLIRADIARLPFASSSVDVV 210
           R  A+   FS+ V    + N  +             ++    ++AD  ++PF  +S D V
Sbjct: 111 REIAR---FSNSVVTGLNNNEYQITRGKELNRLAGVDKTCNFVKADFMKMPFPENSFDAV 170

Query: 211 HAGAAIHCWPSPSAAVAEISRILRPGGVFVASTFIM 240
           +A  A    P       EI R+L+PG  F A  + M
Sbjct: 171 YAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCM 202

BLAST of Chy4G068560 vs. TAIR 10
Match: AT2G40280.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 43.1 bits (100), Expect = 4.5e-04
Identity = 38/159 (23.90%), Postives = 65/159 (40.88%), Query Frame = 0

Query: 90  RLVSFLYERGWRQSFSVLLGFPGPEKEF--------ELIKNFITPVLGGS----IIDASC 149
           +LV +  E+ W +     L FPG   +F        E I+  +  +  G     ++D  C
Sbjct: 154 KLVEYKKEQNWVKKEGEFLVFPGGGTQFKFGVTHYVEFIEKALPSIKWGKNIRVVLDVGC 213

Query: 150 GSGMFSRIFAKSGLFSSVVALDYSENMLRQCYDFIKQEENFPNERLVLIRADIARLPFAS 209
           G       F  S L   V+ + ++     +       E   P    V+      +L F S
Sbjct: 214 GVAS----FGGSLLDKDVITMSFAPKDEHEAQIQFALERGIPATLSVI---GTQQLTFPS 273

Query: 210 SSVDVVH-AGAAIHCWPSPSAAVAEISRILRPGGVFVAS 236
           ++ D++H A   +H        + E++R+LRPGG F+ S
Sbjct: 274 NAFDLIHCARCRVHWDADGGKPLLELNRVLRPGGFFIWS 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LBV42.2e-10465.85Uncharacterized methyltransferase At1g78140, chloroplastic OS=Arabidopsis thalia... [more]
Q0WPT76.1e-7045.70Uncharacterized methyltransferase At2g41040, chloroplastic OS=Arabidopsis thalia... [more]
Q6D3C11.7e-0831.97Malonyl-[acyl-carrier protein] O-methyltransferase OS=Pectobacterium atrosepticu... [more]
E3G3278.6e-0831.91Malonyl-[acyl-carrier protein] O-methyltransferase OS=Enterobacter lignolyticus ... [more]
Q2YY851.1e-0727.89Demethylmenaquinone methyltransferase OS=Staphylococcus aureus (strain bovine RF... [more]
Match NameE-valueIdentityDescription
A0A1S3CQA48.3e-15594.90uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucumis melo OX=36... [more]
A0A6J1IKY52.3e-13682.31uncharacterized methyltransferase At1g78140, chloroplastic-like OS=Cucurbita max... [more]
A0A6J1F9C08.6e-13681.97uncharacterized methyltransferase At1g78140, chloroplastic OS=Cucurbita moschata... [more]
A0A6J1D0A73.7e-13179.73uncharacterized methyltransferase At1g78140, chloroplastic OS=Momordica charanti... [more]
A0A6P4AHU71.2e-11367.65uncharacterized methyltransferase At1g78140, chloroplastic OS=Ziziphus jujuba OX... [more]
Match NameE-valueIdentityDescription
XP_011652625.11.68e-20196.62uncharacterized methyltransferase At1g78140, chloroplastic [Cucumis sativus] >KA... [more]
XP_008466034.13.45e-19794.90PREDICTED: uncharacterized methyltransferase At1g78140, chloroplastic [Cucumis m... [more]
XP_038896627.15.29e-18387.93uncharacterized methyltransferase At1g78140, chloroplastic isoform X2 [Benincasa... [more]
XP_038896626.19.76e-18387.41uncharacterized methyltransferase At1g78140, chloroplastic isoform X1 [Benincasa... [more]
XP_022976042.14.85e-17382.31uncharacterized methyltransferase At1g78140, chloroplastic-like [Cucurbita maxim... [more]
Match NameE-valueIdentityDescription
AT1G78140.11.6e-10565.85S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT2G41040.14.3e-7145.70S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G13710.17.0e-0528.21sterol methyltransferase 1 [more]
AT5G13710.27.0e-0528.21sterol methyltransferase 1 [more]
AT2G40280.14.5e-0423.90S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 134..233
e-value: 1.8E-21
score: 76.5
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 87..298
e-value: 4.3E-32
score: 113.4
NoneNo IPR availablePANTHERPTHR43591:SF46OS08G0411200 PROTEINcoord: 17..299
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 17..299
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 132..239
e-value: 8.81878E-13
score: 61.677
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 91..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G068560.1Chy4G068560.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0006744 ubiquinone biosynthetic process
molecular_function GO:0008168 methyltransferase activity