Chy4G067650 (gene) Cucumber (hystrix) v1

Overview
NameChy4G067650
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein, putative
LocationchrH04: 540332 .. 541928 (+)
RNA-Seq ExpressionChy4G067650
SyntenyChy4G067650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAACCACATTTCAAGATCAATTTTATTTCTTCTTTGTTTTCCAACACTTCTTCAATTAGCATATGGTTTGGACATTGTTCAACACTCTTGCAAATTAGCTGCAAAAACAGACCCATATGTTGATTACAAACTATGTGTACAAACCCTGAAAGCCAATCCCAATTCCAAAGATGCAGAGTTCAAAGACTTGGTTATGATTTCAATCAACCAATCAAAGGCCAATGCCACAGAAATTGGGTCAGAGATTTCAGAGCTAATGAAAAAGAGAAGTGAGAAATGGGGGAAATATTCATTGAATTGTTTGAAGAGCTGTTTAGAGCTTTATTCTGAGGCTGTTTCTGATCTGGAAAAAGCTTTGAGAGGTTTGAAAATGGAGGATTATGAGACAGCAAAAACAGGGGTGAGTGCAGCCATGGATTCACCAGTGAGTTGTGAAGATGGGTACAAGGAGAAAGATGGGGAAGTTTCTCCTTTGTCAGAGATCAATGATGGGTTCTTTCAGTTGGTTGCAATTTCTCTTGCTTTTATCAATATGTGTTAAGTGTCAAGTGATGGATGATGATGCCCTATAACTTAATGGTTTGGAGTTGGGGCTATGAAGAGAAATTTGCTGTGTTTAGAAAGGGTTTTTAGCAATAATATGCATTTTTTTGTGTCTTTTGGTATATGGTATTGTGTTTTGGGAGTGCTAATTAATGTTTCTGTTGTAGAGCAATGTACTACATCGTTTCAAATGTAGACAATTGTTTATGTTGAAAAGTTTTCATAGTACTATTGTAATATTACCTCTATTTGATGTTTGTTATAAAAGAACATTACCTCTATTTGATGTTTGTTATAAAAGAACTATGGTTATATTATAAGCTTTGACACTAAACTTCGCGGTTTGTGACTCTCAGAGTTGATTTTTTTTTTTTTGGTCCTTCAATGGAAAGATTGAAAAGAACGGCAAAATGAGTTTCAACCTTCTCTGGATCCAAAACAATAATATACTGGTATTTACTAGTATCAACACAATGGAGATAGTCGATCAATATAGACTAATCCAAAATTTAATTCTCCAATAAAACAATAAATAGTGTTATTTGCTAATAACAACCTAATGAATTCCTCCCCCCTGCATCCCAGCCCAGCCACAAAAATTTTTATCTCAGAGCAAATTTAGGCCGATTAAGTACCTGTTTGAATATTCTTGGTAGGTTTGGAAGGCAGAATTCAGCATGGAACTAAACAAGTTAATGAGTGAAAAACAAAAATCTGGGAATTATTTGAGAAATTCGTACCTGATACAACCATCTGCATCGAAAAATATACCAAAATCCTGTATGAAAAAATCAACAGAAAAGATATACCTGCAACTAAAATGCTGTATGATTCTCTATTCGAAAAATTAATGGCCAAGTACATCAGATGGCAAAATCAACCTTTTAGTATCCCAACCACCCAATTGATCCACGTCAAAATTAGAGTGACTGCTCCTTGCCACATAGGAAACAGAACCTCCGAATTTAAGGAGGAGAATATGTAACCCATACCTGATACAATGCCGTAATTTCTGATACAGATCACCGAGACGGTTGTCGTATCGTTAA

mRNA sequence

ATGGAAAACCACATTTCAAGATCAATTTTATTTCTTCTTTGTTTTCCAACACTTCTTCAATTAGCATATGGTTTGGACATTGTTCAACACTCTTGCAAATTAGCTGCAAAAACAGACCCATATGTTGATTACAAACTATGTGTACAAACCCTGAAAGCCAATCCCAATTCCAAAGATGCAGAGTTCAAAGACTTGGTTATGATTTCAATCAACCAATCAAAGGCCAATGCCACAGAAATTGGGTCAGAGATTTCAGAGCTAATGAAAAAGAGAAGTGAGAAATGGGGGAAATATTCATTGAATTGTTTGAAGAGCTGTTTAGAGCTTTATTCTGAGGCTGTTTCTGATCTGGAAAAAGCTTTGAGAGGTTTGAAAATGGAGGATTATGAGACAGCAAAAACAGGGGTGAGTGCAGCCATGGATTCACCAGTGAGTTGTGAAGATGGGTACAAGGAGAAAGATGGGGAAGTTTCTCCTTTGTCAGAGATCAATGATGGGTTCTTTCAATCACCGAGACGGTTGTCGTATCGTTAA

Coding sequence (CDS)

ATGGAAAACCACATTTCAAGATCAATTTTATTTCTTCTTTGTTTTCCAACACTTCTTCAATTAGCATATGGTTTGGACATTGTTCAACACTCTTGCAAATTAGCTGCAAAAACAGACCCATATGTTGATTACAAACTATGTGTACAAACCCTGAAAGCCAATCCCAATTCCAAAGATGCAGAGTTCAAAGACTTGGTTATGATTTCAATCAACCAATCAAAGGCCAATGCCACAGAAATTGGGTCAGAGATTTCAGAGCTAATGAAAAAGAGAAGTGAGAAATGGGGGAAATATTCATTGAATTGTTTGAAGAGCTGTTTAGAGCTTTATTCTGAGGCTGTTTCTGATCTGGAAAAAGCTTTGAGAGGTTTGAAAATGGAGGATTATGAGACAGCAAAAACAGGGGTGAGTGCAGCCATGGATTCACCAGTGAGTTGTGAAGATGGGTACAAGGAGAAAGATGGGGAAGTTTCTCCTTTGTCAGAGATCAATGATGGGTTCTTTCAATCACCGAGACGGTTGTCGTATCGTTAA

Protein sequence

MENHISRSILFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDAEFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKALRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQSPRRLSYR*
Homology
BLAST of Chy4G067650 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.8e-21
Identity = 59/160 (36.88%), Postives = 95/160 (59.38%), Query Frame = 0

Query: 10  LFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDAEFKDLVMIS 69
           +F      LLQ     DIVQ +CK  A+  P V+Y  CV++L A+P S  A+ + L +IS
Sbjct: 9   IFFFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHTADLQGLGVIS 68

Query: 70  INQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKALRGLKMEDY 129
            N +  + ++I + I  ++K + +   K  LN    C+ LY++A S +++A+   K +DY
Sbjct: 69  ANLAIQHGSKIQTFIGRILKSKVDPALKKYLN---DCVGLYADAKSSVQEAIADFKSKDY 128

Query: 130 ETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 170
            +A   +SAA+D  V+CEDG+KEK G VSP+++ N  + Q
Sbjct: 129 ASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQ 165

BLAST of Chy4G067650 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.4e-20
Identity = 56/157 (35.67%), Postives = 91/157 (57.96%), Query Frame = 0

Query: 13  LCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDAEFKDLVMISINQ 72
           L F   +      DIVQ +CK  A+  P V+Y  CV++L A+P S  A+ + L +IS N 
Sbjct: 3   LSFSLCIFFLISADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHSADLQGLGVISANL 62

Query: 73  SKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKALRGLKMEDYETA 132
           +    ++I + I  ++K + +   K  LN    C+ LY++A S +++A+   K +DY +A
Sbjct: 63  AIQQGSKIQTFIGRILKSKVDPALKKYLN---DCVGLYADAKSSVQEAIADFKSKDYASA 122

Query: 133 KTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 170
              +SAA+D  V+CEDG+KEK G  SP+++ N  + Q
Sbjct: 123 NVKMSAALDDSVTCEDGFKEKKGIASPVTKENKDYVQ 156

BLAST of Chy4G067650 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 4.8e-14
Identity = 50/147 (34.01%), Postives = 79/147 (53.74%), Query Frame = 0

Query: 27  IVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA-EFKDLVMISINQSKANATEIGSEIS 86
           ++Q SCK A   DP + Y  CV +L  +P SK A   + LV+ S   + A  T +   ++
Sbjct: 23  LIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLASTKTAAAKITNLKGIVA 82

Query: 87  ELMKKRSEKWGKYS--LNCLKSCLELYSEAVSDLEKALRGLKMEDYETAKTGVSAAMDSP 146
           + +K +     +Y   +  LK CL  Y++A  DL  AL  +K  DY+ A   +SAA+D P
Sbjct: 83  QDLKDQ-----RYQDIVEDLKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALDVP 142

Query: 147 VSCEDGYKEKDGEVSPLSEINDGFFQS 171
            +CED +KE   + SP++  N   F++
Sbjct: 143 GNCEDDFKEAK-KTSPITNENSILFKT 163

BLAST of Chy4G067650 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 2.8e-06
Identity = 37/124 (29.84%), Postives = 60/124 (48.39%), Query Frame = 0

Query: 47  CVQTLKANPNSKDAEFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSC 106
           C   +K+NP +  A+ + L  I+   ++ +A+E   +I  L+K  +    K       SC
Sbjct: 43  CTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTATNPTMK---KAYTSC 102

Query: 107 LELYSEAVSDLEKALRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDG 166
           ++ Y  A+S L  A + L   D +     VSAAM+ P +CE      D +V P +  N G
Sbjct: 103 VQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDM--ADFKVDPSAVKNSG 161

Query: 167 FFQS 171
            FQ+
Sbjct: 163 DFQN 161

BLAST of Chy4G067650 vs. ExPASy TrEMBL
Match: A0A0A0LHE9 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910710 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 1.7e-83
Identity = 159/169 (94.08%), Postives = 166/169 (98.22%), Query Frame = 0

Query: 1   MENHISRSILFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA 60
           MEN  SRSILFLLC PTL+QLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKA+PNSKDA
Sbjct: 1   MENPTSRSILFLLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPNSKDA 60

Query: 61  EFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKA 120
           EFKDLV+ISINQSKANATEIGSEISELMK+RSEKWG+YSLNCLKSCLELYSEAVSDLEKA
Sbjct: 61  EFKDLVVISINQSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEKA 120

Query: 121 LRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 170
           L+GLKMEDYETAKTGVSAAMD+PVSCEDGYKEKDGEVSPLSEINDGFFQ
Sbjct: 121 LKGLKMEDYETAKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFFQ 169

BLAST of Chy4G067650 vs. ExPASy TrEMBL
Match: A0A5A7TAE5 (Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004030 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 5.7e-79
Identity = 152/169 (89.94%), Postives = 161/169 (95.27%), Query Frame = 0

Query: 1   MENHISRSILFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA 60
           MEN I++SILFLLC PTL+QLA GLDIVQH CKLAAKTDPYVDYKLCVQTLKANPNS+DA
Sbjct: 1   MENPIAKSILFLLCVPTLIQLANGLDIVQHYCKLAAKTDPYVDYKLCVQTLKANPNSEDA 60

Query: 61  EFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKA 120
           +FKDLVMISINQ+KANATEI SEISELMK+ SEKWGKYSLNCLKSCLELYSEAVSDL+KA
Sbjct: 61  DFKDLVMISINQAKANATEIRSEISELMKRTSEKWGKYSLNCLKSCLELYSEAVSDLKKA 120

Query: 121 LRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 170
           LRGLKMEDYETAKT VSAAMD+PVSCEDGYKEKDGEVSPLSE NDGFFQ
Sbjct: 121 LRGLKMEDYETAKTAVSAAMDAPVSCEDGYKEKDGEVSPLSETNDGFFQ 169

BLAST of Chy4G067650 vs. ExPASy TrEMBL
Match: A0A1S3CQ13 (putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103503498 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 5.7e-79
Identity = 152/169 (89.94%), Postives = 161/169 (95.27%), Query Frame = 0

Query: 1   MENHISRSILFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA 60
           MEN I++SILFLLC PTL+QLA GLDIVQH CKLAAKTDPYVDYKLCVQTLKANPNS+DA
Sbjct: 1   MENPIAKSILFLLCVPTLIQLANGLDIVQHYCKLAAKTDPYVDYKLCVQTLKANPNSEDA 60

Query: 61  EFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKA 120
           +FKDLVMISINQ+KANATEI SEISELMK+ SEKWGKYSLNCLKSCLELYSEAVSDL+KA
Sbjct: 61  DFKDLVMISINQAKANATEIRSEISELMKRTSEKWGKYSLNCLKSCLELYSEAVSDLKKA 120

Query: 121 LRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 170
           LRGLKMEDYETAKT VSAAMD+PVSCEDGYKEKDGEVSPLSE NDGFFQ
Sbjct: 121 LRGLKMEDYETAKTAVSAAMDAPVSCEDGYKEKDGEVSPLSETNDGFFQ 169

BLAST of Chy4G067650 vs. ExPASy TrEMBL
Match: A0A6J1ICI2 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 1.6e-57
Identity = 126/172 (73.26%), Postives = 140/172 (81.40%), Query Frame = 0

Query: 1   MENHISRSI-LFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKD 60
           M++ IS  I L LL  PT   LA+ LDI+Q SCK AAKTDPYVDYKLCVQTL+ANPNSK 
Sbjct: 1   MKSPISNPILLLLLSLPTPFHLAFSLDIIQRSCKSAAKTDPYVDYKLCVQTLRANPNSKA 60

Query: 61  AEFKDLVMISINQSKANATEIGSEISELMKKRSEKW--GKYSLNCLKSCLELYSEAVSDL 120
           A FK+LV+ISI QSKANATEIGSEISEL+ K SE+W  GKYSL CLK CLE+YSEAVS L
Sbjct: 61  ATFKELVLISITQSKANATEIGSEISELL-KGSERWGKGKYSLGCLKGCLEIYSEAVSGL 120

Query: 121 EKALRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 170
           E AL  +KM DYETA   VSAAMD+ +SCEDGYKEKDGEVSPL+EIN GFFQ
Sbjct: 121 ELALGAVKMGDYETANIKVSAAMDAALSCEDGYKEKDGEVSPLTEINCGFFQ 171

BLAST of Chy4G067650 vs. ExPASy TrEMBL
Match: A0A6J1H1W1 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 1.8e-56
Identity = 118/169 (69.82%), Postives = 136/169 (80.47%), Query Frame = 0

Query: 1   MENHISRSILFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA 60
           M+ H S SIL L    TL Q  + LDIVQ SCKLAAK DPYVDYKLCV++L+ANP+SKDA
Sbjct: 1   MKTHFSTSILMLFSLLTLFQFGFCLDIVQRSCKLAAKRDPYVDYKLCVRSLRANPSSKDA 60

Query: 61  EFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKA 120
            FKDLV+ISI QSKANAT IGSEI+ L+ K   KWGKYS +CL+SCLE+YSEAVSDL+  
Sbjct: 61  TFKDLVLISIEQSKANATAIGSEITGLL-KGGGKWGKYSTSCLESCLEVYSEAVSDLKLG 120

Query: 121 LRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 170
           L  +KM DYETA T VSAAMD+P++CEDGY EKDGEVSPL+E N GFFQ
Sbjct: 121 LVAVKMGDYETANTEVSAAMDAPLTCEDGYNEKDGEVSPLTEKNGGFFQ 168

BLAST of Chy4G067650 vs. NCBI nr
Match: XP_004148520.1 (putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 320 bits (820), Expect = 1.39e-109
Identity = 159/169 (94.08%), Postives = 166/169 (98.22%), Query Frame = 0

Query: 1   MENHISRSILFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA 60
           MEN  SRSILFLLC PTL+QLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKA+PNSKDA
Sbjct: 1   MENPTSRSILFLLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPNSKDA 60

Query: 61  EFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKA 120
           EFKDLV+ISINQSKANATEIGSEISELMK+RSEKWG+YSLNCLKSCLELYSEAVSDLEKA
Sbjct: 61  EFKDLVVISINQSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEKA 120

Query: 121 LRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 169
           L+GLKMEDYETAKTGVSAAMD+PVSCEDGYKEKDGEVSPLSEINDGFFQ
Sbjct: 121 LKGLKMEDYETAKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFFQ 169

BLAST of Chy4G067650 vs. NCBI nr
Match: KAE8651458.1 (hypothetical protein Csa_001325 [Cucumis sativus])

HSP 1 Score: 318 bits (815), Expect = 8.35e-109
Identity = 158/168 (94.05%), Postives = 165/168 (98.21%), Query Frame = 0

Query: 1   MENHISRSILFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA 60
           MEN  SRSILFLLC PTL+QLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKA+PNSKDA
Sbjct: 1   MENPTSRSILFLLCVPTLIQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKASPNSKDA 60

Query: 61  EFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKA 120
           EFKDLV+ISINQSKANATEIGSEISELMK+RSEKWG+YSLNCLKSCLELYSEAVSDLEKA
Sbjct: 61  EFKDLVVISINQSKANATEIGSEISELMKRRSEKWGQYSLNCLKSCLELYSEAVSDLEKA 120

Query: 121 LRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFF 168
           L+GLKMEDYETAKTGVSAAMD+PVSCEDGYKEKDGEVSPLSEINDGFF
Sbjct: 121 LKGLKMEDYETAKTGVSAAMDAPVSCEDGYKEKDGEVSPLSEINDGFF 168

BLAST of Chy4G067650 vs. NCBI nr
Match: XP_008465919.1 (PREDICTED: putative invertase inhibitor [Cucumis melo] >KAA0038527.1 putative invertase inhibitor [Cucumis melo var. makuwa] >TYK31124.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 305 bits (781), Expect = 1.23e-103
Identity = 152/169 (89.94%), Postives = 161/169 (95.27%), Query Frame = 0

Query: 1   MENHISRSILFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA 60
           MEN I++SILFLLC PTL+QLA GLDIVQH CKLAAKTDPYVDYKLCVQTLKANPNS+DA
Sbjct: 1   MENPIAKSILFLLCVPTLIQLANGLDIVQHYCKLAAKTDPYVDYKLCVQTLKANPNSEDA 60

Query: 61  EFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKA 120
           +FKDLVMISINQ+KANATEI SEISELMK+ SEKWGKYSLNCLKSCLELYSEAVSDL+KA
Sbjct: 61  DFKDLVMISINQAKANATEIRSEISELMKRTSEKWGKYSLNCLKSCLELYSEAVSDLKKA 120

Query: 121 LRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 169
           LRGLKMEDYETAKT VSAAMD+PVSCEDGYKEKDGEVSPLSE NDGFFQ
Sbjct: 121 LRGLKMEDYETAKTAVSAAMDAPVSCEDGYKEKDGEVSPLSETNDGFFQ 169

BLAST of Chy4G067650 vs. NCBI nr
Match: XP_038888305.1 (putative invertase inhibitor [Benincasa hispida])

HSP 1 Score: 288 bits (736), Expect = 9.14e-97
Identity = 144/169 (85.21%), Postives = 155/169 (91.72%), Query Frame = 0

Query: 1   MENHISRSILFLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA 60
           M++  ++SILFLLC PTL QLAY LDIVQHSCKLAAKTDPYVDYKLCVQ LKANPNSKDA
Sbjct: 1   MKDLTTKSILFLLCVPTLFQLAYCLDIVQHSCKLAAKTDPYVDYKLCVQILKANPNSKDA 60

Query: 61  EFKDLVMISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKA 120
            FKDLVMISINQSKANATEI SEIS+L+K  +EKWGKYSLNCLKSCLELYSEAVSDL+KA
Sbjct: 61  NFKDLVMISINQSKANATEIRSEISQLIKGTNEKWGKYSLNCLKSCLELYSEAVSDLKKA 120

Query: 121 LRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 169
           LR L+MEDYET  T VSAAMD+PVSCEDGYKEKDGEVSPL+EINDGFFQ
Sbjct: 121 LRALEMEDYETTNTEVSAAMDAPVSCEDGYKEKDGEVSPLTEINDGFFQ 169

BLAST of Chy4G067650 vs. NCBI nr
Match: XP_022973258.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 234 bits (596), Expect = 2.03e-75
Identity = 126/172 (73.26%), Postives = 140/172 (81.40%), Query Frame = 0

Query: 1   MENHISRSILFLLC-FPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKD 60
           M++ IS  IL LL   PT   LA+ LDI+Q SCK AAKTDPYVDYKLCVQTL+ANPNSK 
Sbjct: 1   MKSPISNPILLLLLSLPTPFHLAFSLDIIQRSCKSAAKTDPYVDYKLCVQTLRANPNSKA 60

Query: 61  AEFKDLVMISINQSKANATEIGSEISELMKKRSEKWGK--YSLNCLKSCLELYSEAVSDL 120
           A FK+LV+ISI QSKANATEIGSEISEL+K  SE+WGK  YSL CLK CLE+YSEAVS L
Sbjct: 61  ATFKELVLISITQSKANATEIGSEISELLK-GSERWGKGKYSLGCLKGCLEIYSEAVSGL 120

Query: 121 EKALRGLKMEDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 169
           E AL  +KM DYETA   VSAAMD+ +SCEDGYKEKDGEVSPL+EIN GFFQ
Sbjct: 121 ELALGAVKMGDYETANIKVSAAMDAALSCEDGYKEKDGEVSPLTEINCGFFQ 171

BLAST of Chy4G067650 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 105.9 bits (263), Expect = 3.4e-23
Identity = 62/163 (38.04%), Postives = 98/163 (60.12%), Query Frame = 0

Query: 9   ILFLLCFPTLLQ-LAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA-EFKDLV 68
           +L+L+ F  L   LA G  ++++SCK A  T P   Y LCV +L+ NP +K A +   LV
Sbjct: 4   LLYLVTFFVLSNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLETNPQAKTAKDLAGLV 63

Query: 69  MISINQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKALRGLKM 128
           M S   +   AT +   + +++K   +K  K +   L+ CL+LY++A+  L +AL G+K 
Sbjct: 64  MASTKNAVTKATTLKGTVDKIIK--GKKVNKMTAMPLRDCLQLYTDAIGSLNEALAGVKS 123

Query: 129 EDYETAKTGVSAAMDSPVSCEDGYKEKDGEVSPLSEINDGFFQ 170
            +Y T KT +SAAMD+P +CE G+KE+    SP+++ ND  +Q
Sbjct: 124 RNYPTVKTVLSAAMDTPSTCETGFKERKAP-SPVTKENDNLYQ 163

BLAST of Chy4G067650 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 98.6 bits (244), Expect = 5.4e-21
Identity = 60/160 (37.50%), Postives = 91/160 (56.88%), Query Frame = 0

Query: 11  FLLCFPTLLQLAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDAEFKD-LVMIS 70
           F + F  L        ++Q  CK AA  +P + Y  CV++L+ NP SK A   D LVM+S
Sbjct: 7   FAMFFLLLNAFTIAQSLIQDFCKKAADKNPKIHYNFCVKSLEENPQSKTARSLDRLVMLS 66

Query: 71  INQSKANATEIGSEISELMKKRSEKWGKYSLNCLKSCLELYSEAVSDLEKALRGLKMEDY 130
              + +  T +   + +++K+   ++  YS   L+ CLELYS+A + L++AL  +K  DY
Sbjct: 67  TKNAVSKTTSMKGIVDKILKE--NRFEMYSEKPLRDCLELYSDATNSLKEALTIIKSRDY 126

Query: 131 ETAKTGVSAAMDSPVSCEDGYKE-KDGEVSPLSEINDGFF 169
           +TA   +SAAM +P SCE G+KE K    SP ++ ND  F
Sbjct: 127 KTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

BLAST of Chy4G067650 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 3.3e-18
Identity = 59/163 (36.20%), Postives = 91/163 (55.83%), Query Frame = 0

Query: 10  LFLLCFPTLLQ-LAYGLDIVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDAE-FKDLVM 69
           L+L+ F  LL   A    +++ SCK A   DP + Y  CV++L+ NP SK A+  + LV 
Sbjct: 5   LYLVMFFLLLNCFATAQSLIRDSCKKATTKDPKLKYDFCVKSLEENPQSKTAKSLEGLVF 64

Query: 70  ISINQSKANATEIGSEISELMKKRSEKWGKYSL-NCLKSCLELYSEAVSDLEKALRGLKM 129
           +S   + +  T +   + +++K+      KY +   L  CLELY+EA+  L ++L  +K 
Sbjct: 65  VSTKNAVSKTTSLKGMVDKILKE-----DKYEVERPLLDCLELYTEAIDSLNQSLDTVKS 124

Query: 130 EDYETAKTGVSAAMDSPVSCEDGY-KEKDGEVSPLSEINDGFF 169
            DY+TA   +SAAMD+P SCE  + K K    SP ++ ND  F
Sbjct: 125 RDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLF 162

BLAST of Chy4G067650 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 79.3 bits (194), Expect = 3.4e-15
Identity = 50/147 (34.01%), Postives = 79/147 (53.74%), Query Frame = 0

Query: 27  IVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA-EFKDLVMISINQSKANATEIGSEIS 86
           ++Q SCK A   DP + Y  CV +L  +P SK A   + LV+ S   + A  T +   ++
Sbjct: 23  LIQDSCKKAFAKDPQLSYDFCVNSLTQDPQSKAATTLESLVLASTKTAAAKITNLKGIVA 82

Query: 87  ELMKKRSEKWGKYS--LNCLKSCLELYSEAVSDLEKALRGLKMEDYETAKTGVSAAMDSP 146
           + +K +     +Y   +  LK CL  Y++A  DL  AL  +K  DY+ A   +SAA+D P
Sbjct: 83  QDLKDQ-----RYQDIVEDLKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALDVP 142

Query: 147 VSCEDGYKEKDGEVSPLSEINDGFFQS 171
            +CED +KE   + SP++  N   F++
Sbjct: 143 GNCEDDFKEAK-KTSPITNENSILFKT 163

BLAST of Chy4G067650 vs. TAIR 10
Match: AT5G46950.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.3e-14
Identity = 48/147 (32.65%), Postives = 80/147 (54.42%), Query Frame = 0

Query: 27  IVQHSCKLAAKTDPYVDYKLCVQTLKANPNSKDA-EFKDLVMISINQSKANATEIGSEIS 86
           ++Q SCK A   DP   Y  CVQ+L  +P SK A   +DL + S     A  T +   ++
Sbjct: 23  LIQDSCKKAFAKDPQSSYDFCVQSLTQDPQSKAATTLEDLALASTKNVAAKITNLKGIVA 82

Query: 87  ELMKKRSEKWGKYS--LNCLKSCLELYSEAVSDLEKALRGLKMEDYETAKTGVSAAMDSP 146
           + +K +     +Y   +  LK CL  Y +A   L+ AL  +K  DY+ A + +SAA+++P
Sbjct: 83  QDLKDQ-----RYQDIVEDLKLCLGFYKDANDSLKTALANIKSRDYDGANSNLSAALNAP 142

Query: 147 VSCEDGYKEKDGEVSPLSEINDGFFQS 171
             CED +KE + + SP++  N+  +++
Sbjct: 143 GDCEDDFKEAE-KKSPITNENNILYKT 163

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GT411.8e-2136.88Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1[more]
A9YUH43.4e-2035.67Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1[more]
Q9FJR54.8e-1434.01Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1[more]
Q9LUV12.8e-0629.84Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LHE91.7e-8394.08PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G910710 PE=4 S... [more]
A0A5A7TAE55.7e-7989.94Putative invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
A0A1S3CQ135.7e-7989.94putative invertase inhibitor OS=Cucumis melo OX=3656 GN=LOC103503498 PE=4 SV=1[more]
A0A6J1ICI21.6e-5773.26putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111471816 PE=4 SV... [more]
A0A6J1H1W11.8e-5669.82putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111459677 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_004148520.11.39e-10994.08putative invertase inhibitor [Cucumis sativus][more]
KAE8651458.18.35e-10994.05hypothetical protein Csa_001325 [Cucumis sativus][more]
XP_008465919.11.23e-10389.94PREDICTED: putative invertase inhibitor [Cucumis melo] >KAA0038527.1 putative in... [more]
XP_038888305.19.14e-9785.21putative invertase inhibitor [Benincasa hispida][more]
XP_022973258.12.03e-7573.26putative invertase inhibitor [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G46940.13.4e-2338.04Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46970.15.4e-2137.50Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46930.13.3e-1836.20Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46960.13.4e-1534.01Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
AT5G46950.11.3e-1432.65Plant invertase/pectin methylesterase inhibitor superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 107..127
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 141..177
NoneNo IPR availablePANTHERPTHR31080:SF239PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEINcoord: 18..169
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 18..169
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 23..167
e-value: 5.3E-22
score: 89.1
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 27..168
e-value: 5.8E-27
score: 94.9
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 6..169
e-value: 2.7E-31
score: 106.2
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 25..171
e-value: 5.9E-33
score: 116.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 25..169
IPR034088Pla a 1-likeCDDcd15795PMEI-Pla_a_1_likecoord: 32..169
e-value: 1.06271E-40
score: 132.489

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy4G067650.1Chy4G067650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
molecular_function GO:0004857 enzyme inhibitor activity