Chy4G067250 (gene) Cucumber (hystrix) v1

Overview


Name	Chy4G067250
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	membrane steroid-binding protein 2
Location	chrH04: 109024 .. 110845 (+)
RNA-Seq Expression	Chy4G067250
Synteny	Chy4G067250

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCTGCAAGTCTGGGAGACGCTGAAGGAGGCCATCCTCGCCTACACAGGCCTCTCTCCTTCCACCTTCTTCACTGTATTGGCTCTTGGATTTGCCATTTACTATCTCATTTCCAGTTTCTTCGCTCCCTCCGACTACGGAACCCACCCTAGGGACTTGGACCAAATCCATCCTCTCCCTCCTCCTGTTCAACTTGGTGAAATTTCTGAGGATGACTTGAAGCAATACGATGGCTCCGATTCCCAGAAACCTCTTCTCATGGCTATCAAGGGCCAGATCTACGATGTTTCTCAGAGCAGGTTGGTTCATTCAGTTCACCTTTTTCTTACATTTTCCCTCTGTTTTAGGTATTTTGTTGTTTGTATGATCCATCGTGATAACTTCTGATTCCGTTTCAATTTGTTTTTTAGTCAACCTGTTTGCTCACTCCTGCTTTGCTTTGCCCTCTACACCTCTCCTTTTCCTTCAAATTGCTGCTGATTCCTTCAGAATGAAACCTATTTTGGTCCTACTGTATTCAACTTCTATCCTCGTTTTTACGCTATTTCTAATTCCTTCTCTTTTCCTGTTTCTTATAACTATGACCCATGCATTGTGTGCTTGATGCAAGCTATAATAAAAGAGGTCTATTGATAGCTTTTGTGCAGTTTATGTTTCTTTCTGGTTGTACGAGCTGAAAACGGAATTGACTCTTTTAGGTCGATTTTATTTCCATGGCTTTGTGGATTTCTGTAGTGACCAGTTTACAAGTTACAATGAATATCTTTAATGATGTTATATGGTTGTCATAATATATTTGATTTTCGCTTTATGGATATCAATTCAAACTTTCAACTTTGACTCTGATGTTTGTGTTTCTTTGTTACTTGTAGCTGTGATTTGCTATGTATCTGGTTTTGTAAATATTTTACTGGGACTGTCGTTTCTTATTTCAAATTAGGGCTATGGTGTTTCCAGCATATAGAATTCGTATAGTATATGCAAGACAGAGGCAATAGTTCCGTGTTTCAACTTCCACGAGTTATTCTGAAACTTCTATGGTTTCAAATCACTTTTTAGGAACTCATGATTTCGAATCACTTTTTACAAGCTTATGATGTCCTAAAAAGTAGCGAGTTTCTTTTCGAAAAACACTATAAGGACAGGTCCATTGTTTCATGATTATGTATACACTTGGACATGCATTAAATTTGTTGTGACATCTTTGGCTATCTGACTTCATGAGGAGTTTTTCTGACTATGTATTATTCACTACACCAAGGCTGTAGACACAAATATCCCAATATTTTGTTAGTGTCTTGCTCTCTGTCTCTTGTGTGTGAGTCCTCTTAAAGGAAAATTTAAATTGGAGAACATCGCACTTGTACCCTTCAGGTTTTAGTCTTGTGTACGCCCATAGATGGAGCTTAATTTTCCATATTTATCTCAATCAAGCTTTCATATTTCTTTGCTATTATTTCTTCTAACCTGCACTATTTGCAGGATGTTCTATGGACCCGGGGGGCCCTATGCCCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCATTTGAAGAGAAAGATCTGACTGGGGATACCTCTGGTCTCGGTCCATCCGAACTGGAAATGTTGCAGGATTGGGAGTACAAATTCATGAGTAAATATGTTAAAGTTGGGACAGTGAAGAAGTCAGTTGCTGAAGGTGATGCTGCAGCTGGTGAGCCTGCAGAATCAAGCTCTGCTGATGTTAGTAAGCCTGTAGAAGAAAGTCACTCAGAACCTCGAGATGATAAAGCTGAGGAAGCCCCTGCTGCTTCAAATGTCGATTAA

mRNA sequence

ATGGCTCTGCAAGTCTGGGAGACGCTGAAGGAGGCCATCCTCGCCTACACAGGCCTCTCTCCTTCCACCTTCTTCACTGTATTGGCTCTTGGATTTGCCATTTACTATCTCATTTCCAGTTTCTTCGCTCCCTCCGACTACGGAACCCACCCTAGGGACTTGGACCAAATCCATCCTCTCCCTCCTCCTGTTCAACTTGGTGAAATTTCTGAGGATGACTTGAAGCAATACGATGGCTCCGATTCCCAGAAACCTCTTCTCATGGCTATCAAGGGCCAGATCTACGATGTTTCTCAGAGCAGGATGTTCTATGGACCCGGGGGGCCCTATGCCCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCATTTGAAGAGAAAGATCTGACTGGGGATACCTCTGGTCTCGGTCCATCCGAACTGGAAATGTTGCAGGATTGGGAGTACAAATTCATGAGTAAATATGTTAAAGTTGGGACAGTGAAGAAGTCAGTTGCTGAAGGTGATGCTGCAGCTGGTGAGCCTGCAGAATCAAGCTCTGCTGATGTTAGTAAGCCTGTAGAAGAAAGTCACTCAGAACCTCGAGATGATAAAGCTGAGGAAGCCCCTGCTGCTTCAAATGTCGATTAA

Coding sequence (CDS)

ATGGCTCTGCAAGTCTGGGAGACGCTGAAGGAGGCCATCCTCGCCTACACAGGCCTCTCTCCTTCCACCTTCTTCACTGTATTGGCTCTTGGATTTGCCATTTACTATCTCATTTCCAGTTTCTTCGCTCCCTCCGACTACGGAACCCACCCTAGGGACTTGGACCAAATCCATCCTCTCCCTCCTCCTGTTCAACTTGGTGAAATTTCTGAGGATGACTTGAAGCAATACGATGGCTCCGATTCCCAGAAACCTCTTCTCATGGCTATCAAGGGCCAGATCTACGATGTTTCTCAGAGCAGGATGTTCTATGGACCCGGGGGGCCCTATGCCCTTTTTGCTGGAAAGGATGCCAGCAGAGCTCTTGCAAAAATGTCATTTGAAGAGAAAGATCTGACTGGGGATACCTCTGGTCTCGGTCCATCCGAACTGGAAATGTTGCAGGATTGGGAGTACAAATTCATGAGTAAATATGTTAAAGTTGGGACAGTGAAGAAGTCAGTTGCTGAAGGTGATGCTGCAGCTGGTGAGCCTGCAGAATCAAGCTCTGCTGATGTTAGTAAGCCTGTAGAAGAAAGTCACTCAGAACCTCGAGATGATAAAGCTGAGGAAGCCCCTGCTGCTTCAAATGTCGATTAA

Protein sequence

MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDLDQIHPLPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAAAGEPAESSSADVSKPVEESHSEPRDDKAEEAPAASNVD*

Homology

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BLAST of Chy4G067250 vs. ExPASy Swiss-Prot
Match: Q9M2Z4 (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 6.8e-68
Identity = 134/187 (71.66%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDLD---QI 60
           M  Q+WETLKE I AYTGLSP+ FFTVLAL FA+Y ++S FF   +    PR L+   Q 
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEV-HRPRSLEVQPQS 60

Query: 61  HPLPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD 120
            PLPPPVQLGEI+E++LK YDGSDS+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD
Sbjct: 61  EPLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD 120

Query: 121 ASRALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAAAGE 180
           ASRALAKMSFE++DLTGD SGLG  ELE LQDWEYKFMSKYVKVGT++K   EG  ++ E
Sbjct: 121 ASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESS-E 180

Query: 181 PAESSSA 185
           P+E+ +A
Sbjct: 181 PSEAKTA 185

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BLAST of Chy4G067250 vs. ExPASy Swiss-Prot
Match: Q9FVZ7 (Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP1 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 6.4e-66
Identity = 138/219 (63.01%), Postives = 162/219 (73.97%), Query Frame = 0


Query: 4   QVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFA--PSDYGTHPRDLDQIHPLP 63
           ++WETLK+AI+AYTGLSP+ FFT +A   A+Y+++S  FA  P      PRD  +  PLP
Sbjct: 7   ELWETLKQAIVAYTGLSPAAFFTAVAAAAALYHVVSGIFAGPPPPPPPRPRDEPEAEPLP 66

Query: 64  PPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRA 123
           PPVQLGE+SE++L+QYDGSD +KPLLMAIKGQIYDV+QSRMFYGPGGPYALFAGKDASRA
Sbjct: 67  PPVQLGEVSEEELRQYDGSDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAGKDASRA 126

Query: 124 LAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGD--------- 183
           LAKMSFE +DLTGD SGLGP EL+ LQDWEYKFM KYVKVGTVKK+V   D         
Sbjct: 127 LAKMSFEPQDLTGDISGLGPFELDALQDWEYKFMGKYVKVGTVKKTVPVEDGAPSTSPET 186

Query: 184 ---AAAGEPAESSSADVSKPVEESHSEPRDDKAEEAPAA 209
              AAA EP E + A   KP E S  E ++ +   A AA
Sbjct: 187 TETAAAAEP-EKAPATEEKPREVSSEEVKEKEDAVAAAA 224

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BLAST of Chy4G067250 vs. ExPASy Swiss-Prot
Match: Q9XFM6 (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 245.7 bits (626), Expect = 4.6e-64
Identity = 138/214 (64.49%), Postives = 162/214 (75.70%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAP--SDYGTHPR----DL 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL FAIY +IS +FA    D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  DQIHPLPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           ++  P+P PVQ+GEI+E++LKQYDGSD QKPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGD-A 180
           GKDASRALAKMSFEEKDLT D SGLGP EL+ LQDWEYKFMSKY KVGTVK + +E + A
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPETA 180

Query: 181 AAGEPAESSSADVSKPVEESHSEPRDDKAEEAPA 208
           +  EP E+   D    V  +  +   DK+++APA
Sbjct: 181 SVSEPTENVEQDAH--VTTTPGKTVVDKSDDAPA 212

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BLAST of Chy4G067250 vs. ExPASy Swiss-Prot
Match: Q9FVZ9 (Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=MSBP2 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 7.4e-46
Identity = 101/167 (60.48%), Postives = 119/167 (71.26%), Query Frame = 0


Query: 20  SPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRD--LDQIHPLPPPVQLGEISEDDLKQY 79
           SP      +A    +Y + SS  AP      PR+   ++  P P PVQ+GEI+ ++L QY
Sbjct: 70  SPVAVIAAVAGAAVVYKVGSSLLAPPPPPARPREEPSEEAPPPPEPVQVGEITAEELLQY 129

Query: 80  DGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEEKDLTGDTS 139
           DGSD +KPLLMAIKGQIYDVSQSR+FYGPGGPYALFAGKDASRALAKMSFE +DLT D S
Sbjct: 130 DGSDPEKPLLMAIKGQIYDVSQSRLFYGPGGPYALFAGKDASRALAKMSFEPQDLTDDIS 189

Query: 140 GLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAE--GDAAAGEPAESS 183
           GL   EL  LQDWEYKF SKYVKVGT+KK + E  GD+ A    E++
Sbjct: 190 GLSLLELSALQDWEYKFSSKYVKVGTIKKVLVEQGGDSTADAIEEAA 236

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BLAST of Chy4G067250 vs. ExPASy Swiss-Prot
Match: Q9SK39 (Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.5e-22
Identity = 49/97 (50.52%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 68  EISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 127
           E + + L QY+G+D  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 128 EEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTV 165
            E+D++    GL   E+  L DWE KF +KY  VG V
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRV 98

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BLAST of Chy4G067250 vs. ExPASy TrEMBL
Match: A0A0A0LIG2 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G915110 PE=4 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 3.2e-108
Identity = 204/212 (96.23%), Postives = 208/212 (98.11%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDLDQIHPL 60
           MALQVW+TLKEAILAYTGLSPSTFFTVLALG AIYYLISSFFAPSDYGTHPRDLDQIHPL
Sbjct: 1   MALQVWDTLKEAILAYTGLSPSTFFTVLALGLAIYYLISSFFAPSDYGTHPRDLDQIHPL 60

Query: 61  PPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASR 120
           PPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASR
Sbjct: 61  PPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASR 120

Query: 121 ALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAAAGEPAE 180
           ALAKMSFEEKDLTGD SGLGPSELEMLQDWEYKFMSKYVKVGTVK SVAEGDAAAGEPAE
Sbjct: 121 ALAKMSFEEKDLTGDISGLGPSELEMLQDWEYKFMSKYVKVGTVKTSVAEGDAAAGEPAE 180

Query: 181 SSSADVSKPVEESHSEPRDDKAEEAPAASNVD 213
           SS+A+VSKPVEESHSEPRDDKAEEAPAA+N D
Sbjct: 181 SSTAEVSKPVEESHSEPRDDKAEEAPAAANAD 212

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BLAST of Chy4G067250 vs. ExPASy TrEMBL
Match: A0A5A7TA90 (Membrane steroid-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6571G00110 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.5e-107
Identity = 204/213 (95.77%), Postives = 211/213 (99.06%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPS-DYGTHPRDLDQIHP 60
           MALQVWETLKEAI+AYTGLSPSTFFTVLALGFAIYYLISSFFAPS DYGTH RDL+QIHP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGFAIYYLISSFFAPSADYGTHARDLEQIHP 60

Query: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQLGEISEDDLKQYDG+DS+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEISEDDLKQYDGTDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAAAGEPA 180
           RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKK+VAEGDAAAGEPA
Sbjct: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKTVAEGDAAAGEPA 180

Query: 181 ESSSADVSKPVEESHSEPRDDKAEEAPAASNVD 213
           ESSSA+VSKPVEESHSEPRDDKAEEAPAA+NVD
Sbjct: 181 ESSSAEVSKPVEESHSEPRDDKAEEAPAAANVD 213

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BLAST of Chy4G067250 vs. ExPASy TrEMBL
Match: A0A1S3CQ79 (membrane steroid-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103503452 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.5e-107
Identity = 204/213 (95.77%), Postives = 211/213 (99.06%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPS-DYGTHPRDLDQIHP 60
           MALQVWETLKEAI+AYTGLSPSTFFTVLALGFAIYYLISSFFAPS DYGTH RDL+QIHP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGFAIYYLISSFFAPSADYGTHARDLEQIHP 60

Query: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQLGEISEDDLKQYDG+DS+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEISEDDLKQYDGTDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAAAGEPA 180
           RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKK+VAEGDAAAGEPA
Sbjct: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKTVAEGDAAAGEPA 180

Query: 181 ESSSADVSKPVEESHSEPRDDKAEEAPAASNVD 213
           ESSSA+VSKPVEESHSEPRDDKAEEAPAA+NVD
Sbjct: 181 ESSSAEVSKPVEESHSEPRDDKAEEAPAAANVD 213

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BLAST of Chy4G067250 vs. ExPASy TrEMBL
Match: A0A6J1I394 (membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470596 PE=4 SV=1)

HSP 1 Score: 339.0 bits (868), Expect = 1.5e-89
Identity = 181/215 (84.19%), Postives = 193/215 (89.77%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDL-DQIHP 60
           MALQVWETLKEAI+AYTGLSPSTFFTVLALG A YYLISSFF PSDYGTH RDL +Q+ P
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE+SED+LKQYDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQIGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDA--AAGE 180
           RALAKMSFEEKDLTGD SGLGP ELE LQDWEYKFMSKYVKVGT+KK VAEGDA  AAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSSADV--SKPVEESHSEPRDDK-AEEAPAAS 210
           PAESS+A+V  SKP E+S SE   DK AEE PAA+
Sbjct: 181 PAESSTAEVSSSKPAEDSPSESHADKAAEETPAAA 215

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BLAST of Chy4G067250 vs. ExPASy TrEMBL
Match: A0A6J1GMC2 (membrane steroid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111455271 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 3.3e-89
Identity = 181/217 (83.41%), Postives = 193/217 (88.94%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDL-DQIHP 60
           MALQVWETLKEAI+AYTGLSPSTFFTVLALG A YYLISSFF PSDYGTH RDL +Q+ P
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE+SED+LKQYDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDA--AAGE 180
           RALAKMSFEEKDLTGD SGLGP ELE LQDWEYKFMSKYVKVGT+KK VAEGDA  AAGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSSADV----SKPVEESHSEPRDDK-AEEAPAAS 210
           PAESS+A+V    SKP E+S SE   DK AEE PAA+
Sbjct: 181 PAESSTAEVSSSSSKPAEDSPSESHADKAAEETPAAA 217

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BLAST of Chy4G067250 vs. NCBI nr
Match: XP_004148349.1 (membrane steroid-binding protein 1 [Cucumis sativus] >KGN60482.1 hypothetical protein Csa_001989 [Cucumis sativus])

HSP 1 Score: 400 bits (1029), Expect = 2.37e-140
Identity = 204/212 (96.23%), Postives = 208/212 (98.11%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDLDQIHPL 60
           MALQVW+TLKEAILAYTGLSPSTFFTVLALG AIYYLISSFFAPSDYGTHPRDLDQIHPL
Sbjct: 1   MALQVWDTLKEAILAYTGLSPSTFFTVLALGLAIYYLISSFFAPSDYGTHPRDLDQIHPL 60

Query: 61  PPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASR 120
           PPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASR
Sbjct: 61  PPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASR 120

Query: 121 ALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAAAGEPAE 180
           ALAKMSFEEKDLTGD SGLGPSELEMLQDWEYKFMSKYVKVGTVK SVAEGDAAAGEPAE
Sbjct: 121 ALAKMSFEEKDLTGDISGLGPSELEMLQDWEYKFMSKYVKVGTVKTSVAEGDAAAGEPAE 180

Query: 181 SSSADVSKPVEESHSEPRDDKAEEAPAASNVD 212
           SS+A+VSKPVEESHSEPRDDKAEEAPAA+N D
Sbjct: 181 SSTAEVSKPVEESHSEPRDDKAEEAPAAANAD 212

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BLAST of Chy4G067250 vs. NCBI nr
Match: XP_008465863.1 (PREDICTED: membrane steroid-binding protein 2 [Cucumis melo] >KAA0038487.1 membrane steroid-binding protein 2 [Cucumis melo var. makuwa] >TYK03336.1 membrane steroid-binding protein 2 [Cucumis melo var. makuwa])

HSP 1 Score: 397 bits (1020), Expect = 5.79e-139
Identity = 204/213 (95.77%), Postives = 211/213 (99.06%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPS-DYGTHPRDLDQIHP 60
           MALQVWETLKEAI+AYTGLSPSTFFTVLALGFAIYYLISSFFAPS DYGTH RDL+QIHP
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGFAIYYLISSFFAPSADYGTHARDLEQIHP 60

Query: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQLGEISEDDLKQYDG+DS+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQLGEISEDDLKQYDGTDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAAAGEPA 180
           RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKK+VAEGDAAAGEPA
Sbjct: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKTVAEGDAAAGEPA 180

Query: 181 ESSSADVSKPVEESHSEPRDDKAEEAPAASNVD 212
           ESSSA+VSKPVEESHSEPRDDKAEEAPAA+NVD
Sbjct: 181 ESSSAEVSKPVEESHSEPRDDKAEEAPAAANVD 213

[Close]

BLAST of Chy4G067250 vs. NCBI nr
Match: XP_038888418.1 (membrane steroid-binding protein 2-like [Benincasa hispida])

HSP 1 Score: 369 bits (948), Expect = 5.46e-128
Identity = 190/213 (89.20%), Postives = 200/213 (93.90%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDL-DQIHP 60
           MALQVWETLKEAI+AYTGLSP+TFFTVLALG AIYYLISSFFAPSDYGTH RDL +Q+ P
Sbjct: 1   MALQVWETLKEAIVAYTGLSPATFFTVLALGIAIYYLISSFFAPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GEISEDDLKQYDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEISEDDLKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAAAGEPA 180
           RALAKMSFEEKDLTGD SGLGP ELE LQDWEYKFMSKYVKVGTV+KSVA+GDAAAGEPA
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTVQKSVADGDAAAGEPA 180

Query: 181 ESSSADVSKPVEESHSEPRDDKAEEAPAASNVD 212
           ESS A+VSKP EESHSE RDDK EE PAA+NVD
Sbjct: 181 ESSIAEVSKPAEESHSESRDDKVEETPAAANVD 213

[Close]

BLAST of Chy4G067250 vs. NCBI nr
Match: XP_022971922.1 (membrane steroid-binding protein 2-like [Cucurbita maxima])

HSP 1 Score: 338 bits (868), Expect = 1.04e-115
Identity = 181/215 (84.19%), Postives = 193/215 (89.77%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDL-DQIHP 60
           MALQVWETLKEAI+AYTGLSPSTFFTVLALG A YYLISSFF PSDYGTH RDL +Q+ P
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE+SED+LKQYDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQIGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAA--AGE 180
           RALAKMSFEEKDLTGD SGLGP ELE LQDWEYKFMSKYVKVGT+KK VAEGDAA  AGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSSADVS--KPVEESHSEPRDDKA-EEAPAAS 209
           PAESS+A+VS  KP E+S SE   DKA EE PAA+
Sbjct: 181 PAESSTAEVSSSKPAEDSPSESHADKAAEETPAAA 215

[Close]

BLAST of Chy4G067250 vs. NCBI nr
Match: XP_022952639.1 (membrane steroid-binding protein 2-like [Cucurbita moschata] >XP_022952640.1 membrane steroid-binding protein 2-like [Cucurbita moschata])

HSP 1 Score: 337 bits (865), Expect = 3.20e-115
Identity = 181/217 (83.41%), Postives = 193/217 (88.94%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDL-DQIHP 60
           MALQVWETLKEAI+AYTGLSPSTFFTVLALG A YYLISSFF PSDYGTH RDL +Q+ P
Sbjct: 1   MALQVWETLKEAIVAYTGLSPSTFFTVLALGLAFYYLISSFFGPSDYGTHTRDLGEQLQP 60

Query: 61  LPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120
           LPPPVQ+GE+SED+LKQYDGSD +KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS
Sbjct: 61  LPPPVQVGEVSEDELKQYDGSDPEKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDAS 120

Query: 121 RALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAA--AGE 180
           RALAKMSFEEKDLTGD SGLGP ELE LQDWEYKFMSKYVKVGT+KK VAEGDAA  AGE
Sbjct: 121 RALAKMSFEEKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKPVAEGDAAPAAGE 180

Query: 181 PAESSSADVS----KPVEESHSEPRDDKA-EEAPAAS 209
           PAESS+A+VS    KP E+S SE   DKA EE PAA+
Sbjct: 181 PAESSTAEVSSSSSKPAEDSPSESHADKAAEETPAAA 217

[Close]

BLAST of Chy4G067250 vs. TAIR 10
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3 )

HSP 1 Score: 258.5 bits (659), Expect = 4.9e-69
Identity = 134/187 (71.66%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAPSDYGTHPRDLD---QI 60
           M  Q+WETLKE I AYTGLSP+ FFTVLAL FA+Y ++S FF   +    PR L+   Q 
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEV-HRPRSLEVQPQS 60

Query: 61  HPLPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD 120
            PLPPPVQLGEI+E++LK YDGSDS+KPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD
Sbjct: 61  EPLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD 120

Query: 121 ASRALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGDAAAGE 180
           ASRALAKMSFE++DLTGD SGLG  ELE LQDWEYKFMSKYVKVGT++K   EG  ++ E
Sbjct: 121 ASRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESS-E 180

Query: 181 PAESSSA 185
           P+E+ +A
Sbjct: 181 PSEAKTA 185

[Close]

BLAST of Chy4G067250 vs. TAIR 10
Match: AT5G52240.1 (membrane steroid binding protein 1 )

HSP 1 Score: 245.7 bits (626), Expect = 3.3e-65
Identity = 138/214 (64.49%), Postives = 162/214 (75.70%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAP--SDYGTHPR----DL 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL FAIY +IS +FA    D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  DQIHPLPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           ++  P+P PVQ+GEI+E++LKQYDGSD QKPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVKKSVAEGD-A 180
           GKDASRALAKMSFEEKDLT D SGLGP EL+ LQDWEYKFMSKY KVGTVK + +E + A
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPETA 180

Query: 181 AAGEPAESSSADVSKPVEESHSEPRDDKAEEAPA 208
           +  EP E+   D    V  +  +   DK+++APA
Sbjct: 181 SVSEPTENVEQDAH--VTTTPGKTVVDKSDDAPA 212

[Close]

BLAST of Chy4G067250 vs. TAIR 10
Match: AT5G52240.2 (membrane steroid binding protein 1 )

HSP 1 Score: 243.0 bits (619), Expect = 2.1e-64
Identity = 126/171 (73.68%), Postives = 140/171 (81.87%), Query Frame = 0


Query: 1   MALQVWETLKEAILAYTGLSPSTFFTVLALGFAIYYLISSFFAP--SDYGTHPR----DL 60
           MAL++W+TLKEAI AYTGLSP  FFT LAL FAIY +IS +FA    D   H R      
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  DQIHPLPPPVQLGEISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           ++  P+P PVQ+GEI+E++LKQYDGSD QKPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTVK 166
           GKDASRALAKMSFEEKDLT D SGLGP EL+ LQDWEYKFMSKY KVGTVK
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVK 171

[Close]

BLAST of Chy4G067250 vs. TAIR 10
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2 )

HSP 1 Score: 107.1 bits (266), Expect = 1.8e-23
Identity = 49/97 (50.52%), Postives = 67/97 (69.07%), Query Frame = 0


Query: 68  EISEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 127
           E + + L QY+G+D  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 128 EEKDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVGTV 165
            E+D++    GL   E+  L DWE KF +KY  VG V
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRV 98

[Close]

BLAST of Chy4G067250 vs. TAIR 10
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4 )

HSP 1 Score: 76.3 bits (186), Expect = 3.4e-14
Identity = 36/93 (38.71%), Postives = 53/93 (56.99%), Query Frame = 0


Query: 70  SEDDLKQYDGSDSQKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEE 129
           S ++L  Y+G+D   P+L+ I G ++DV++ +  YG GG Y  FAG+DASRA    +F  
Sbjct: 43  SAEELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTG 102

Query: 130 KDLTGDTSGLGPSELEMLQDWEYKFMSKYVKVG 163
             LT    GL  SE++ + DW   +   Y  VG
Sbjct: 103 DGLTDSLQGLSSSEVKSIVDWRGFYSRTYTPVG 135

The following BLAST results are available for this feature:

BLAST of Chy4G067250 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2Z4	6.8e-68	71.66	Membrane steroid-binding protein 2 OS=Arabidopsis thaliana OX=3702 GN=MSBP2 PE=1...	[more]
Q9FVZ7	6.4e-66	63.01	Membrane steroid-binding protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9XFM6	4.6e-64	64.49	Membrane steroid-binding protein 1 OS=Arabidopsis thaliana OX=3702 GN=MSBP1 PE=1...	[more]
Q9FVZ9	7.4e-46	60.48	Membrane steroid-binding protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=M...	[more]
Q9SK39	2.5e-22	50.52	Probable steroid-binding protein 3 OS=Arabidopsis thaliana OX=3702 GN=MP3 PE=1 S...	[more]

BLAST of Chy4G067250 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LIG2	3.2e-108	96.23	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A5A7TA90	3.5e-107	95.77	Membrane steroid-binding protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3CQ79	3.5e-107	95.77	membrane steroid-binding protein 2 OS=Cucumis melo OX=3656 GN=LOC103503452 PE=4 ...	[more]
A0A6J1I394	1.5e-89	84.19	membrane steroid-binding protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111470...	[more]
A0A6J1GMC2	3.3e-89	83.41	membrane steroid-binding protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of Chy4G067250 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148349.1	2.37e-140	96.23	membrane steroid-binding protein 1 [Cucumis sativus] >KGN60482.1 hypothetical pr...	[more]
XP_008465863.1	5.79e-139	95.77	PREDICTED: membrane steroid-binding protein 2 [Cucumis melo] >KAA0038487.1 membr...	[more]
XP_038888418.1	5.46e-128	89.20	membrane steroid-binding protein 2-like [Benincasa hispida]	[more]
XP_022971922.1	1.04e-115	84.19	membrane steroid-binding protein 2-like [Cucurbita maxima]	[more]
XP_022952639.1	3.20e-115	83.41	membrane steroid-binding protein 2-like [Cucurbita moschata] >XP_022952640.1 mem...	[more]

BLAST of Chy4G067250 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48890.1	4.9e-69	71.66	membrane-associated progesterone binding protein 3	[more]
AT5G52240.1	3.3e-65	64.49	membrane steroid binding protein 1	[more]
AT5G52240.2	2.1e-64	73.68	membrane steroid binding protein 1	[more]
AT2G24940.1	1.8e-23	50.52	membrane-associated progesterone binding protein 2	[more]
AT4G14965.1	3.4e-14	38.71	membrane-associated progesterone binding protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 69..165 e-value: 8.7E-24 score: 95.0
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 71..164 e-value: 2.4E-14 score: 53.2
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 67..165 e-value: 3.4E-36 score: 125.5
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 60..165
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 187..206
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 170..212
None	No IPR available	PANTHER	PTHR10281	MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED	coord: 1..209
None	No IPR available	PANTHER	PTHR10281:SF90	STEROID-BINDING PROTEIN 3-RELATED	coord: 1..209

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy4G067250.1	Chy4G067250.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0019904	protein domain specific binding