Chy3G065860 (gene) Cucumber (hystrix) v1

Overview
NameChy3G065860
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationchrH03: 18012602 .. 18015774 (+)
RNA-Seq ExpressionChy3G065860
SyntenyChy3G065860
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGATGTACCAGCCTGCTACTACTTGGAGAGAAGGGGATCCGAAGCTCGTCGTTGGCTGCGGTACGCAGATCCTAGATCTGGAAACTGTGGTCAAAGATGGGATTCTCGGTGGAGGCGGTGGGATCATATGCGGAGGCGGTGGCGTTGGGGGTGGAGAGAAATTGGATCTGAAGGAGATGATTGAGGAGTTGGAATCGTCTGTAGAGGTTCCATCGGTGTTCATTTGTCCAATCTCTCTCGAGCCCATGCAAGATCCAGTGACCCTCTGCTCAGGCCAAACCTACGAGCGGTCCAACATTCTGAAATGGTTCTCTTTGGGGCATTTAACTTGTCCCACAACAATGCAAGAGCTTTGGGACGATTCCGTCACACCGAATCGGACCCTTCACCAGTTGATTCACACCTGGTTTTCCCAGAAGTATTTAGCATTGAAGAAGAGATCAGAGGATGTGCAAGGGAGAGTTTTAGAGCTGTTGGATTCGTTGAAAAAGGTCAAAGGTCAAGCTAGGGTTCAGTCCCTCAAAGATCTCCGACATATCGTCATTACCCATTCCTCCGCTAAGAAGATGGTGGTCGACAACGGCGGTGTCGCTCTAATATCTTCTCTTTTGGGTTCTTTCACTTCCCACGCAGTTGGGTCCGAAGCCTTGGGAATCCTCGTGAATTTAGATCTAAATTCAGAGTTAAAGAAGAATCTGATTCAGCCCACCAGAATATCTCTAATTGCAGATATATTAAACGAAGGGTCCATCGAAACCAAGATTAATTGTACAAAATTCTTCAAGATGTTGATCGTGGGTGAGGATTTAAAAACGGAGGACGTTTCGAGTTTAAGTCTCCTCGTCGGATTGTTGAGAATGGTGAAGGATCAGAGACACCCAAATGGTGCAACCGCCGGACTCAGTCTACTCCGAACACTCTGTTCAAACGAACCAATCAGGAAGCAACTCGTTAGCATCGGCGCCGTTGCGAAACTCGGCGGCACCCTCTCTAACTTAAACACCGATTGCTTGGAATCGGCGCTTTATATTCTCGACGTTTTGTCATCCCTTCCAGAGGGGAGATTAGCGTTGAAGAATTGTGAAGATACGATTAGAAATTTGGTGAAATTGTTGATGAAAGTATCAGAGATTTGTACTCAATTTGCGTTGTCAATTTTATGGGCAGTGTTCAAGCTAGCACCAGAGGAATGTGCGTTGCTCGCAGTGGAAGCTGGATTAGCAGCAAAATTGCTGCTTGTCATACAAAGTGGGTGCAATCCAGAGCTGAAACAGAGATCTGCAGAGCTATTAAAATTGTGTAGTCTAAATTACCCAGATTCAATCTTCATTTCTAAATGTAAGCTTACGAGAACTATACAATAAAAGATAGCTTGTCAAAATTGTTCATACACCCCTTTTCTTTTTTTTTTCTTTTCATTTTTGTTTATTATGCATGATTGAGATTCTTCAAACAGTCTTGTGAATGTGAATGAATGCGAGAAGCAAGTTTTGATTCCTGTGTTTTGTTGCTTTTTCTGTTATATGTTTTTCCTCATTTTCTTCAATTGAATTGACGTTTATCGATGACCAGAATATTCTTTTCCGGTATTCTGGCAAGTGTTGATTCTTTGTTCGGGAAAAGGCAGAAATATCAAATAATGCAGATTTGTATTCTTTTTTATTTTCTTTATTATTATTTTTTTGTGGTCAAAGAATCCTGGACAACAGCCAAGCTTCATAATTGATAAACTATGCTTTGACTGAGTTTGGCCACAGAGAAAGAGAGAGAGAGAGAGAAATGAAAGTTATACAAAAGCAAAAACAAAGACTCGTCCCTATAAAACTGTATTTTTTAATGTAGAAAACATTTTGCTTTTTAATTTTACTTTTTTAAAAAGCAAAATTTTCTTGTTATAAGTGATGGCTTATTTTATTTACATTTTTATATATATTTTTTTTTGTTATTACCAATCTCTTCGAAAAGTTCTTCATGCATGATTAAGTTTACTCACCATATTGTTGTTTACTTATCATTATTATATTGAATTGTGTTTCTTCTTGATTATGAATTTGACAATAATAACATAGATCAACATGATTAATTGTTTCTAAATTTTAACTACGTCTAAACTTTGAGGTTATCAAGATGATTAGGATAAAATGAATAAAGCAATAAAAAATTGGTTTATAATATACTTTTGTGTGATGATATGACCCATGTAATTTTGTAGGAGTGAACAAATAAATAATTGAACCTTAAAAACATAAAACAGAAGAAGACAACAATGGTAGGGCCTAAAAAATATTGAAAAGAAAGAAAAGGGCAAAGGAGGAAAAAGAAAAAGGGGGGGAGAGAATCATAGAATGAGAGTGTGAGAATTGATGAACATTTGTTGGTGGATGAACAAGGTTTGGTTTGATTGACTAGTGAAGGTTCAACCACCACTTTAAAATAAAACCACATTGGAGGGTATGAAATGCAGTTGGCCAATTCTTGAATTTTTTGTCTAAGTTTTTTCTCATTTAAAAGTATCAAGTTTTTAATCAATGAGGCTCCCTACCTGCTTTATAAGTAATTAAAAAAATTTCATCTTTTAATAAATGATATAAAAATTAAATAAAAATTAAGCATATATTTCTTTGAAATAAAATTGAGATATTTTTTAGAACTCTCCTTTTAGATATCAAAATATGAAGTCTAATTACTTTTAAAATTCCTACTAATTTCTTCAAACCATACCATTGATTGTCTCAATTCTTATCTCTCTCTCTCTCTAGCATATTAAATTGAATAAATAAACCCATTCATTTGGTATTGTTTCTTCCTTGTTTCTCTCTCTATCTCTTCATCTCTCTCTTCATTATACCCAAATTGAACTGCCCCTCTGCTTTTGGCTGTCTGTGACTGCACAGTATACTATTTTCAATATATATATATATATATATATAATTTTATTAATAATTTGAACATTTAATTTGAAATAATCTGAAATAGAATAATGTCTTTGAAAAGTGTGTGTATGTGTTTTACAATTTTATTCTTGTTTAGTGTGCTTTTGCCGAGTGTTTGCCACGTGAACACCTCGACTTTCAGCATTGAAAAAAAAAAAAAAAAAGGTTAAAAGAATGAAAATTTAGGATTTAAAATTATGGTTTCCTTTTTAGCAGCACGGACTTTTGGGAAATGA

mRNA sequence

ATGCCGATGTACCAGCCTGCTACTACTTGGAGAGAAGGGGATCCGAAGCTCGTCGTTGGCTGCGGTACGCAGATCCTAGATCTGGAAACTGTGGTCAAAGATGGGATTCTCGGTGGAGGCGGTGGGATCATATGCGGAGGCGGTGGCGTTGGGGGTGGAGAGAAATTGGATCTGAAGGAGATGATTGAGGAGTTGGAATCGTCTGTAGAGGTTCCATCGGTGTTCATTTGTCCAATCTCTCTCGAGCCCATGCAAGATCCAGTGACCCTCTGCTCAGGCCAAACCTACGAGCGGTCCAACATTCTGAAATGGTTCTCTTTGGGGCATTTAACTTGTCCCACAACAATGCAAGAGCTTTGGGACGATTCCGTCACACCGAATCGGACCCTTCACCAGTTGATTCACACCTGGTTTTCCCAGAAGTATTTAGCATTGAAGAAGAGATCAGAGGATGTGCAAGGGAGAGTTTTAGAGCTGTTGGATTCGTTGAAAAAGGTCAAAGGTCAAGCTAGGGTTCAGTCCCTCAAAGATCTCCGACATATCGTCATTACCCATTCCTCCGCTAAGAAGATGGTGGTCGACAACGGCGGTGTCGCTCTAATATCTTCTCTTTTGGGTTCTTTCACTTCCCACGCAGTTGGGTCCGAAGCCTTGGGAATCCTCGTGAATTTAGATCTAAATTCAGAGTTAAAGAAGAATCTGATTCAGCCCACCAGAATATCTCTAATTGCAGATATATTAAACGAAGGGTCCATCGAAACCAAGATTAATTGTACAAAATTCTTCAAGATGTTGATCGTGGGTGAGGATTTAAAAACGGAGGACGTTTCGAGTTTAAGTCTCCTCGTCGGATTGTTGAGAATGGTGAAGGATCAGAGACACCCAAATGGTGCAACCGCCGGACTCAGTCTACTCCGAACACTCTGTTCAAACGAACCAATCAGGAAGCAACTCGTTAGCATCGGCGCCGTTGCGAAACTCGGCGGCACCCTCTCTAACTTAAACACCGATTGCTTGGAATCGGCGCTTTATATTCTCGACGTTTTGTCATCCCTTCCAGAGGGGAGATTAGCGTTGAAGAATTGTGAAGATACGATTAGAAATTTGGTGAAATTGTTGATGAAAGTATCAGAGATTTGTACTCAATTTGCGTTGTCAATTTTATGGGCAGTGTTCAAGCTAGCACCAGAGGAATGTGCGTTGCTCGCAGTGGAAGCTGGATTAGCAGCAAAATTGCTGCTTGTCATACAAAGTGGGTGCAATCCAGAGCTGAAACAGAGATCTGCAGAGCTATTAAAATTGTGTAGTCTAAATTACCCAGATTCAATCTTCATTTCTAAATCACGGACTTTTGGGAAATGA

Coding sequence (CDS)

ATGCCGATGTACCAGCCTGCTACTACTTGGAGAGAAGGGGATCCGAAGCTCGTCGTTGGCTGCGGTACGCAGATCCTAGATCTGGAAACTGTGGTCAAAGATGGGATTCTCGGTGGAGGCGGTGGGATCATATGCGGAGGCGGTGGCGTTGGGGGTGGAGAGAAATTGGATCTGAAGGAGATGATTGAGGAGTTGGAATCGTCTGTAGAGGTTCCATCGGTGTTCATTTGTCCAATCTCTCTCGAGCCCATGCAAGATCCAGTGACCCTCTGCTCAGGCCAAACCTACGAGCGGTCCAACATTCTGAAATGGTTCTCTTTGGGGCATTTAACTTGTCCCACAACAATGCAAGAGCTTTGGGACGATTCCGTCACACCGAATCGGACCCTTCACCAGTTGATTCACACCTGGTTTTCCCAGAAGTATTTAGCATTGAAGAAGAGATCAGAGGATGTGCAAGGGAGAGTTTTAGAGCTGTTGGATTCGTTGAAAAAGGTCAAAGGTCAAGCTAGGGTTCAGTCCCTCAAAGATCTCCGACATATCGTCATTACCCATTCCTCCGCTAAGAAGATGGTGGTCGACAACGGCGGTGTCGCTCTAATATCTTCTCTTTTGGGTTCTTTCACTTCCCACGCAGTTGGGTCCGAAGCCTTGGGAATCCTCGTGAATTTAGATCTAAATTCAGAGTTAAAGAAGAATCTGATTCAGCCCACCAGAATATCTCTAATTGCAGATATATTAAACGAAGGGTCCATCGAAACCAAGATTAATTGTACAAAATTCTTCAAGATGTTGATCGTGGGTGAGGATTTAAAAACGGAGGACGTTTCGAGTTTAAGTCTCCTCGTCGGATTGTTGAGAATGGTGAAGGATCAGAGACACCCAAATGGTGCAACCGCCGGACTCAGTCTACTCCGAACACTCTGTTCAAACGAACCAATCAGGAAGCAACTCGTTAGCATCGGCGCCGTTGCGAAACTCGGCGGCACCCTCTCTAACTTAAACACCGATTGCTTGGAATCGGCGCTTTATATTCTCGACGTTTTGTCATCCCTTCCAGAGGGGAGATTAGCGTTGAAGAATTGTGAAGATACGATTAGAAATTTGGTGAAATTGTTGATGAAAGTATCAGAGATTTGTACTCAATTTGCGTTGTCAATTTTATGGGCAGTGTTCAAGCTAGCACCAGAGGAATGTGCGTTGCTCGCAGTGGAAGCTGGATTAGCAGCAAAATTGCTGCTTGTCATACAAAGTGGGTGCAATCCAGAGCTGAAACAGAGATCTGCAGAGCTATTAAAATTGTGTAGTCTAAATTACCCAGATTCAATCTTCATTTCTAAATCACGGACTTTTGGGAAATGA

Protein sequence

MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKEMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGCNPELKQRSAELLKLCSLNYPDSIFISKSRTFGK*
Homology
BLAST of Chy3G065860 vs. ExPASy Swiss-Prot
Match: Q058P4 (U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 4.4e-149
Identity = 279/450 (62.00%), Postives = 352/450 (78.22%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVG-GGEKLDLK 60
           MPM+QP    R+G      G   Q+LDL+T VKDG+LGG       GGGVG   EKLDLK
Sbjct: 1   MPMFQPLK--RDGLIGFEGGGDGQVLDLDTAVKDGVLGG-----VNGGGVGVVDEKLDLK 60

Query: 61  EMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQEL 120
           +MI+EL+   ++PSVFICPISLEPMQDPVTLC+GQTYER NI KWF+LGHLTCPTTMQEL
Sbjct: 61  KMIKELDLQ-DIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQEL 120

Query: 121 WDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLR 180
           WDD+VTPN+TLH LI+TWFSQKY+ +KKRSEDVQGR +E+L +LKK KGQARV +L +L+
Sbjct: 121 WDDTVTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELK 180

Query: 181 HIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTR 240
            IVI H  A+K VV+ GGV++ISSLLG FTSHAVGSE + ILV+LDL+S+ K  L+QP +
Sbjct: 181 QIVIAHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAK 240

Query: 241 ISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGAT 300
           +SLI D+LN+GS ETKINC +  + L+  +  + E VSS SLLVGL+R+VKD+RH NG +
Sbjct: 241 VSLIVDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVS 300

Query: 301 AGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLAL 360
             L LL+ +  ++ +R  +VSIGAV +L   L +L+ +CLE AL++LD L +  EGR+A+
Sbjct: 301 PALRLLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAV 360

Query: 361 KNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSG 420
           K+  +TI   V++LM+VSE CT +ALSILW+V KLAPEEC+ LAVE GLAAKLLLVIQSG
Sbjct: 361 KDSANTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSG 420

Query: 421 CNPELKQRSAELLKLCSLNYPDSIFISKSR 450
           C+  LKQRSAELLKLCSL+Y D++FISK +
Sbjct: 421 CDAALKQRSAELLKLCSLHYSDTMFISKCK 442

BLAST of Chy3G065860 vs. ExPASy Swiss-Prot
Match: Q9FHN9 (U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.1e-147
Identity = 278/452 (61.50%), Postives = 349/452 (77.21%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGG---IICGGGGVGGGEKLD 60
           MPM+QP+   + G      G    ILDL + VKDG+LGGG G   ++         +KLD
Sbjct: 1   MPMFQPS---KNG------GFDGHILDLHSAVKDGVLGGGDGKFLVVV----TDEKKKLD 60

Query: 61  LKEMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQ 120
           LKEMI ELE   E+PSVFICPISLEPMQDPVTLC+GQTYERSNILKWF++GH TCPTTMQ
Sbjct: 61  LKEMISELELP-EIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQ 120

Query: 121 ELWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKD 180
           ELWDD VTPN+TLHQLI+TWFSQKY+ +KKRSEDVQGR +E+L +L+K KG+A+V +L +
Sbjct: 121 ELWDDLVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSE 180

Query: 181 LRHIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQP 240
           L+ +V+ H+ AKK VVD GGV +ISSLL  FTSHAVGSEA+ ILVNL+L+S+ K  L+QP
Sbjct: 181 LKQVVMAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQP 240

Query: 241 TRISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNG 300
            R+SL+ D+LN+GSIETKINC +    L+  +  + E VSS SLLVGL+R+VKD+R  NG
Sbjct: 241 ARVSLMVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNG 300

Query: 301 ATAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRL 360
            +  L+LL+++  ++ +R  LV IGAV +L   L  L+ +CLESAL++LD L    EGR+
Sbjct: 301 VSPALTLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRI 360

Query: 361 ALKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQ 420
           ALK+  +TI + V+LLMKVSE CT +A+SILW+V KLA EEC+ LAVE GLAAKLLLVIQ
Sbjct: 361 ALKDSVNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQ 420

Query: 421 SGCNPELKQRSAELLKLCSLNYPDSIFISKSR 450
           SGC+P LKQRSAELLKLCSL+Y DS+FISK +
Sbjct: 421 SGCDPALKQRSAELLKLCSLHYSDSMFISKCK 438

BLAST of Chy3G065860 vs. ExPASy Swiss-Prot
Match: Q10PI9 (U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=TUD1 PE=1 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 2.7e-130
Identity = 238/445 (53.48%), Postives = 332/445 (74.61%), Query Frame = 0

Query: 21  CGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKEMIEELESSVE------VPSV 80
           CG Q+LD++T +KDGILGGG  +     G GG + ++L++M++EL+++ +      VP+V
Sbjct: 9   CGGQVLDIDTALKDGILGGGPELGDAAAGDGGKQPVELRKMMDELDAAGDGGGDEAVPAV 68

Query: 81  FICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDSVTPNRTLHQLI 140
           FICPISLEPM DPVTLC+GQTYE +NI +W +LGH TCPTTMQELWD +  PN TL QLI
Sbjct: 69  FICPISLEPMVDPVTLCTGQTYESANISRWLALGHRTCPTTMQELWDVTPIPNTTLRQLI 128

Query: 141 HTWFSQKYLALKKRSEDVQGRVLELLDSLK--------KVKGQARVQSLKDLRHIVITHS 200
             WFS++Y   KKRS D  GR  EL+ +L+         +KGQARV +L++LR +   H 
Sbjct: 129 AAWFSRRYTRFKKRSADFHGRAAELVHALRGTAVPKRQPLKGQARVAALRELRSLAAAHQ 188

Query: 201 SAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVN-LDLNSELKKNLIQPTRISLIAD 260
           S  K + + GGV L++SLLG FTSHAVGSEA+ ILV+ + L+++ K  L+QP ++SL+ D
Sbjct: 189 SVTKAIAEAGGVGLLTSLLGPFTSHAVGSEAVAILVSGVPLDADAKAALMQPAKVSLLVD 248

Query: 261 ILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATAGLSLL 320
           +LNEG+++TKINC +  ++L+  +  + + V+SLSLLVG++R+V+D+RHP+G  AGL LL
Sbjct: 249 MLNEGAVDTKINCVRLIRILMEEKGFRPDTVASLSLLVGVMRLVRDKRHPDGVAAGLELL 308

Query: 321 RTLCS-NEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALKNCED 380
            ++C+ ++P R  +VSIGAV +L   L  L T+C+E AL ILD L+++PEGR+ALK+C  
Sbjct: 309 NSICAVHKPARSLIVSIGAVPQLVELLPELPTECVEPALDILDALAAVPEGRIALKDCPR 368

Query: 381 TIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGCNPEL 440
           TI N V+LLM+VSE CT+ ALS+LW V ++APEECA  A++AGL AKLLLVIQSGC PEL
Sbjct: 369 TITNAVRLLMRVSEACTRRALSMLWVVCRMAPEECAPAALDAGLGAKLLLVIQSGCGPEL 428

Query: 441 KQRSAELLKLCSLNYPDSIFISKSR 450
           KQ+++ELLKLC++N   ++FISK +
Sbjct: 429 KQQASELLKLCTMNCTSTVFISKCK 453

BLAST of Chy3G065860 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 1.6e-37
Identity = 129/419 (30.79%), Postives = 204/419 (48.69%), Query Frame = 0

Query: 62  IEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLG-HLTCPTTMQELW 121
           IE L+  +++P  F CPISLE MQDPVT+C+GQTY+R++I  W S+G + TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 122 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQ-----------GRVLELLDSLKKVKG- 181
           D ++ PN TL +LI  W       +  RS  V+             V  LL     + G 
Sbjct: 65  DFTLIPNHTLRRLIQEW------CVANRSNGVERIPTPKQPADPTSVRALLSQASAITGT 124

Query: 182 ----QARVQSLKDLRHIVITHSSAKKMV-VDNGGVALISSLLGSFTSHAVGSEALGILVN 241
               ++R  +L+ LR         + ++   N    LI  L    TS  + SE+L +LV 
Sbjct: 125 HVSVRSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVM 184

Query: 242 LDLN--SELKKNLIQPTRISLIADILNEGSIETKINCTKFFKMLIVG---EDLKTEDVSS 301
           L +   ++       P R+  +  +L + SIET++N     +++  G    DLK    +S
Sbjct: 185 LPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNS 244

Query: 302 LSLLVGLLRMVKDQRHPNGA-TAGLSLLRTLCSNEPIRKQLVSIGA----VAKLGGTLSN 361
            S+  G+L ++++      A   G+  L  LCS +  R   ++ GA    + +L      
Sbjct: 245 ESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 304

Query: 362 LNTDCLESALYILDVLSSLPEGRLALKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFK 421
            +T   E AL  +++L   PEG  A      T+  LVK +++VS+  T++A   L A+  
Sbjct: 305 CDT---ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC- 364

Query: 422 LAPEECALLAVEAGLAAKLLLVIQSGCNPELKQRSAELLKLCSLNYPDSIFISKSRTFG 453
            A E     A  AG+  +LLL++QS C    K+++ +LLKL   ++PD    + S  FG
Sbjct: 365 TAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDFG 413

BLAST of Chy3G065860 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 3.3e-35
Identity = 121/408 (29.66%), Postives = 200/408 (49.02%), Query Frame = 0

Query: 62  IEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWD 121
           +E L+  +++P  F CPISL+ M DPVT+ +GQTY+R++I  W ++G+ TCP T   L D
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSD 64

Query: 122 DSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQG-----------RVLELLDSLKKVKG-- 181
            ++ PN TL +LI  W       +  RS  V+             V  LL     + G  
Sbjct: 65  FTLIPNHTLRRLIQEW------CVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTH 124

Query: 182 ---QARVQSLKDLRHIVITHSSAKKMVVDNGGVALISSLL------GSFTSHAVGSEALG 241
              ++R  +++ LR +       + ++  +    ++  +L       S +S  V SE+L 
Sbjct: 125 VSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV-SESLA 184

Query: 242 ILVNLDL-NSELKKNLIQPTRISLIADILNEGSIETKINCTKFFKMLIVGE---DLKTED 301
           +LV L +  +E +     P+R+  +  +L + SIE ++N     +M++ G    DLK   
Sbjct: 185 LLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLII 244

Query: 302 VSSLSLLVGLLRMVKDQRHPNGA-TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNL 361
             S S+  G+L ++K+      A   G+  +  LC  +  R   +S GA   L   L+  
Sbjct: 245 SGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAAD 304

Query: 362 NTDC-LESALYILDVLSSLPEGRLALKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFK 421
              C  E  L  +++L  LPEG  A      T+  +VK +++VS+  T++A   L A+  
Sbjct: 305 FDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC- 364

Query: 422 LAPEECALLAVEAGLAAKLLLVIQSGCNPELKQRSAELLKLCSLNYPD 442
            A E C   A  AGL  +LLL++QS C    K+++  LLKL   ++PD
Sbjct: 365 TAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPD 404

BLAST of Chy3G065860 vs. ExPASy TrEMBL
Match: A0A0A0LKY4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G354100 PE=4 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 4.8e-247
Identity = 443/449 (98.66%), Postives = 446/449 (99.33%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGG GGGEKLDLKE
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGGGGGEKLDLKE 60

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEA+GILVNLDLNSELKKNLIQPTRI
Sbjct: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNG TA
Sbjct: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
           GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK
Sbjct: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECA+LAVEAGLAAKLLLVIQSGC
Sbjct: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGC 420

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 450
           NPELKQRSAELLKLCSLNYPDSIFISK +
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCK 449

BLAST of Chy3G065860 vs. ExPASy TrEMBL
Match: A0A5A7UYA8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00980 PE=4 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.2e-244
Identity = 439/449 (97.77%), Postives = 443/449 (98.66%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGG GGGEKLDLKE
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGGGGGEKLDLKE 60

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLR 
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRL 180

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEA+GILVNLDLNSELKKNLIQPTRI
Sbjct: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKF KMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNG TA
Sbjct: 241 SLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
           GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLN DCLESALYILDVLSSLPEGRLALK
Sbjct: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLALK 360

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECA+LAVEAGLAAKLLLVIQSGC
Sbjct: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGC 420

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 450
           NPELKQRSAELLKLCSLNYPDSIFISK +
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCK 449

BLAST of Chy3G065860 vs. ExPASy TrEMBL
Match: A0A1S3BA20 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487822 PE=4 SV=1)

HSP 1 Score: 854.4 bits (2206), Expect = 2.2e-244
Identity = 439/449 (97.77%), Postives = 443/449 (98.66%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGG GGGEKLDLKE
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGGGGGEKLDLKE 60

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLR 
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRL 180

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEA+GILVNLDLNSELKKNLIQPTRI
Sbjct: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKF KMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNG TA
Sbjct: 241 SLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
           GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLN DCLESALYILDVLSSLPEGRLALK
Sbjct: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLALK 360

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECA+LAVEAGLAAKLLLVIQSGC
Sbjct: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGC 420

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 450
           NPELKQRSAELLKLCSLNYPDSIFISK +
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCK 449

BLAST of Chy3G065860 vs. ExPASy TrEMBL
Match: A0A6J1HFA1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462914 PE=4 SV=1)

HSP 1 Score: 756.1 bits (1951), Expect = 8.2e-215
Identity = 394/449 (87.75%), Postives = 413/449 (91.98%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MP+Y P+    + DPKL+VGCG  ILDL+TVVKDGILGGGGG +      GG +KLDLKE
Sbjct: 1   MPIYHPSIC-TQRDPKLLVGCGAHILDLQTVVKDGILGGGGGGVIAAD--GGAQKLDLKE 60

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           +V  HSSAKKMVVDNGGVALISSLLGSFTSHAVGSE +GILVNLDLNSELKKNLIQPTRI
Sbjct: 181 VVTAHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEGVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKF KML  G+    E+VSSLSLLVGLLRMVKDQRHPNG TA
Sbjct: 241 SLIADILNEGSIETKINCTKFIKMLTEGDASTIENVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
            L+LLRTLCSNE IRKQLV IGAVAKLGG LS LN + LESALYILDVLSSLPEGRLALK
Sbjct: 301 ALALLRTLCSNESIRKQLVGIGAVAKLGGALSGLNAESLESALYILDVLSSLPEGRLALK 360

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           NCEDT+RNLVKLLMKVSEICT+FALSILWAVFKLAPEEC++LAVEAGLAAKLLLVIQSG 
Sbjct: 361 NCEDTVRNLVKLLMKVSEICTRFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGF 420

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 450
           NPELKQRSAELLKLCSLNYPDSIFISK +
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCK 446

BLAST of Chy3G065860 vs. ExPASy TrEMBL
Match: A0A6J1K7W0 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492478 PE=4 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 2.6e-213
Identity = 392/449 (87.31%), Postives = 411/449 (91.54%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MP+Y P+    + DPKL+V CG  ILDL+TVVKDGILGGGGG +      GG +KLDLKE
Sbjct: 1   MPIYHPSIC-TQRDPKLLVDCGAHILDLQTVVKDGILGGGGGGVIATD--GGAQKLDLKE 60

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           +V  HSS KKMVVDNGGVALISSLLGSFTSHAVGSE +GILVNLDLNSELKKNLIQPTRI
Sbjct: 181 VVTAHSSVKKMVVDNGGVALISSLLGSFTSHAVGSEGVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKF KML  G+    E+VSSLSLLVGLLRMVKDQRHPNG TA
Sbjct: 241 SLIADILNEGSIETKINCTKFIKMLTEGDASTIENVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
            L+LLRTLCSNE IRKQLV IGAVAKLGG LS LN + LESALYILDVLSSLPEGRLALK
Sbjct: 301 ALTLLRTLCSNESIRKQLVGIGAVAKLGGALSGLNAESLESALYILDVLSSLPEGRLALK 360

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           NCEDT+RNLVKLLMKVSEICT+FALSILWAVFKLAPEEC++LAVEAGLAAKLLLVIQSG 
Sbjct: 361 NCEDTVRNLVKLLMKVSEICTRFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGF 420

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 450
           NPELKQRSAELLKLCSLNYPDSIFISK +
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCK 446

BLAST of Chy3G065860 vs. NCBI nr
Match: XP_031736908.1 (U-box domain-containing protein 30 isoform X2 [Cucumis sativus])

HSP 1 Score: 858 bits (2216), Expect = 3.97e-313
Identity = 443/449 (98.66%), Postives = 446/449 (99.33%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGG GGGEKLDLKE
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGGGGGEKLDLKE 60

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEA+GILVNLDLNSELKKNLIQPTRI
Sbjct: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNG TA
Sbjct: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
           GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK
Sbjct: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECA+LAVEAGLAAKLLLVIQSGC
Sbjct: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGC 420

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 449
           NPELKQRSAELLKLCSLNYPDSIFISK +
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCK 449

BLAST of Chy3G065860 vs. NCBI nr
Match: XP_011649559.1 (U-box domain-containing protein 30 isoform X1 [Cucumis sativus] >KAE8652062.1 hypothetical protein Csa_018635 [Cucumis sativus])

HSP 1 Score: 858 bits (2216), Expect = 9.46e-313
Identity = 443/449 (98.66%), Postives = 446/449 (99.33%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGG GGGEKLDLKE
Sbjct: 24  MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGGGGGEKLDLKE 83

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW
Sbjct: 84  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 143

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH
Sbjct: 144 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 203

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEA+GILVNLDLNSELKKNLIQPTRI
Sbjct: 204 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPTRI 263

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNG TA
Sbjct: 264 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGVTA 323

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
           GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK
Sbjct: 324 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 383

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECA+LAVEAGLAAKLLLVIQSGC
Sbjct: 384 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGC 443

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 449
           NPELKQRSAELLKLCSLNYPDSIFISK +
Sbjct: 444 NPELKQRSAELLKLCSLNYPDSIFISKCK 472

BLAST of Chy3G065860 vs. NCBI nr
Match: XP_008444531.1 (PREDICTED: U-box domain-containing protein 30-like [Cucumis melo] >KAA0060963.1 U-box domain-containing protein 30-like [Cucumis melo var. makuwa])

HSP 1 Score: 849 bits (2193), Expect = 1.27e-309
Identity = 439/449 (97.77%), Postives = 443/449 (98.66%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGG GGGEKLDLKE
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGGGGGEKLDLKE 60

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLR 
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRL 180

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEA+GILVNLDLNSELKKNLIQPTRI
Sbjct: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKF KMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNG TA
Sbjct: 241 SLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
           GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLN DCLESALYILDVLSSLPEGRLALK
Sbjct: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLALK 360

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECA+LAVEAGLAAKLLLVIQSGC
Sbjct: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGC 420

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 449
           NPELKQRSAELLKLCSLNYPDSIFISK +
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCK 449

BLAST of Chy3G065860 vs. NCBI nr
Match: XP_038884660.1 (U-box domain-containing protein 31-like [Benincasa hispida])

HSP 1 Score: 823 bits (2125), Expect = 2.32e-299
Identity = 427/449 (95.10%), Postives = 437/449 (97.33%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MPMYQPAT WREGDPKLVVGCG QILDLETVVKDGILGGGGGIICGGG     EKLDLK+
Sbjct: 1   MPMYQPAT-WREGDPKLVVGCGAQILDLETVVKDGILGGGGGIICGGG-----EKLDLKD 60

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIEELESS+EVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           +V THSSAKKMVVDNGGVALISSLLGSFTSHAVGSEA+GILVNLDL+SELKKNLIQPTRI
Sbjct: 181 VVTTHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLDSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKF KMLIVGEDL+TE+VSSLSLLVGLLRMVKDQRHPNG TA
Sbjct: 241 SLIADILNEGSIETKINCTKFIKMLIVGEDLRTENVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
           GLSLLRTLCSNEPIRKQLVSIGAVAKLGG LSNLNTDCLESALYILDVLSSLPEGRLALK
Sbjct: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGALSNLNTDCLESALYILDVLSSLPEGRLALK 360

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEEC++LAVEAGLAAKLLLVIQSGC
Sbjct: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECSVLAVEAGLAAKLLLVIQSGC 420

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 449
           NPELKQRSAELLKLCSLNYPDSIFISK +
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCK 443

BLAST of Chy3G065860 vs. NCBI nr
Match: XP_023536979.1 (U-box domain-containing protein 30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 753 bits (1945), Expect = 6.57e-272
Identity = 390/449 (86.86%), Postives = 419/449 (93.32%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVGGGEKLDLKE 60
           MPMYQP+T WREGDPKLVV CG QILDLETVVKDGILGGGGG+   GGG GGGEKLDLKE
Sbjct: 1   MPMYQPST-WREGDPKLVVACGAQILDLETVVKDGILGGGGGV---GGGGGGGEKLDLKE 60

Query: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120
           MIE LESS+EVPSVFICPISLEPMQDPVTLCSGQTYER NI KWFSLGHLTCPTTMQELW
Sbjct: 61  MIEVLESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELW 120

Query: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180
           DDS+TPNRTLHQLIHTWFSQK+LA KK+SEDVQGRV ELLDSLKKVKGQARVQSLKDLRH
Sbjct: 121 DDSLTPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRH 180

Query: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTRI 240
           +V  HSSAKKMVVDNGGVA+ISSLLGSFTSHAVGSEA+GILVNLDL+SELK NLIQPTRI
Sbjct: 181 VVAAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGATA 300
           SLIADILNEGSIETKINCTKF K L  G+D +TE VSSLSLLVGLLRMVKDQ+HPNG TA
Sbjct: 241 SLIADILNEGSIETKINCTKFIKKLTDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTA 300

Query: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360
           GLSLLR+LCSNE I+KQLVSIGAVAKLGG LS+ N D LESALYIL+VLSSLPEGRLALK
Sbjct: 301 GLSLLRSLCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILEVLSSLPEGRLALK 360

Query: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSGC 420
           +CE+TI+ LVKLLMKVSEICT+FALSILWAVFK+APEECA++AV+AGLAAKLLLVIQSGC
Sbjct: 361 DCEETIKKLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGC 420

Query: 421 NPELKQRSAELLKLCSLNYPDSIFISKSR 449
           N E+KQRS+ELLKLC+LNYPDSIFISK +
Sbjct: 421 NKEVKQRSSELLKLCTLNYPDSIFISKCK 445

BLAST of Chy3G065860 vs. TAIR 10
Match: AT3G49810.1 (ARM repeat superfamily protein )

HSP 1 Score: 529.3 bits (1362), Expect = 3.1e-150
Identity = 279/450 (62.00%), Postives = 352/450 (78.22%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGVG-GGEKLDLK 60
           MPM+QP    R+G      G   Q+LDL+T VKDG+LGG       GGGVG   EKLDLK
Sbjct: 1   MPMFQPLK--RDGLIGFEGGGDGQVLDLDTAVKDGVLGG-----VNGGGVGVVDEKLDLK 60

Query: 61  EMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQEL 120
           +MI+EL+   ++PSVFICPISLEPMQDPVTLC+GQTYER NI KWF+LGHLTCPTTMQEL
Sbjct: 61  KMIKELDLQ-DIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQEL 120

Query: 121 WDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLR 180
           WDD+VTPN+TLH LI+TWFSQKY+ +KKRSEDVQGR +E+L +LKK KGQARV +L +L+
Sbjct: 121 WDDTVTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELK 180

Query: 181 HIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQPTR 240
            IVI H  A+K VV+ GGV++ISSLLG FTSHAVGSE + ILV+LDL+S+ K  L+QP +
Sbjct: 181 QIVIAHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAK 240

Query: 241 ISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGAT 300
           +SLI D+LN+GS ETKINC +  + L+  +  + E VSS SLLVGL+R+VKD+RH NG +
Sbjct: 241 VSLIVDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVS 300

Query: 301 AGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLAL 360
             L LL+ +  ++ +R  +VSIGAV +L   L +L+ +CLE AL++LD L +  EGR+A+
Sbjct: 301 PALRLLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAV 360

Query: 361 KNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQSG 420
           K+  +TI   V++LM+VSE CT +ALSILW+V KLAPEEC+ LAVE GLAAKLLLVIQSG
Sbjct: 361 KDSANTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSG 420

Query: 421 CNPELKQRSAELLKLCSLNYPDSIFISKSR 450
           C+  LKQRSAELLKLCSL+Y D++FISK +
Sbjct: 421 CDAALKQRSAELLKLCSLHYSDTMFISKCK 442

BLAST of Chy3G065860 vs. TAIR 10
Match: AT5G65920.1 (ARM repeat superfamily protein )

HSP 1 Score: 524.6 bits (1350), Expect = 7.7e-149
Identity = 278/452 (61.50%), Postives = 349/452 (77.21%), Query Frame = 0

Query: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGG---IICGGGGVGGGEKLD 60
           MPM+QP+   + G      G    ILDL + VKDG+LGGG G   ++         +KLD
Sbjct: 1   MPMFQPS---KNG------GFDGHILDLHSAVKDGVLGGGDGKFLVVV----TDEKKKLD 60

Query: 61  LKEMIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQ 120
           LKEMI ELE   E+PSVFICPISLEPMQDPVTLC+GQTYERSNILKWF++GH TCPTTMQ
Sbjct: 61  LKEMISELELP-EIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQ 120

Query: 121 ELWDDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKD 180
           ELWDD VTPN+TLHQLI+TWFSQKY+ +KKRSEDVQGR +E+L +L+K KG+A+V +L +
Sbjct: 121 ELWDDLVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSE 180

Query: 181 LRHIVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEALGILVNLDLNSELKKNLIQP 240
           L+ +V+ H+ AKK VVD GGV +ISSLL  FTSHAVGSEA+ ILVNL+L+S+ K  L+QP
Sbjct: 181 LKQVVMAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQP 240

Query: 241 TRISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNG 300
            R+SL+ D+LN+GSIETKINC +    L+  +  + E VSS SLLVGL+R+VKD+R  NG
Sbjct: 241 ARVSLMVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNG 300

Query: 301 ATAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRL 360
            +  L+LL+++  ++ +R  LV IGAV +L   L  L+ +CLESAL++LD L    EGR+
Sbjct: 301 VSPALTLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRI 360

Query: 361 ALKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECALLAVEAGLAAKLLLVIQ 420
           ALK+  +TI + V+LLMKVSE CT +A+SILW+V KLA EEC+ LAVE GLAAKLLLVIQ
Sbjct: 361 ALKDSVNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQ 420

Query: 421 SGCNPELKQRSAELLKLCSLNYPDSIFISKSR 450
           SGC+P LKQRSAELLKLCSL+Y DS+FISK +
Sbjct: 421 SGCDPALKQRSAELLKLCSLHYSDSMFISKCK 438

BLAST of Chy3G065860 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 158.7 bits (400), Expect = 1.1e-38
Identity = 129/419 (30.79%), Postives = 204/419 (48.69%), Query Frame = 0

Query: 62  IEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLG-HLTCPTTMQELW 121
           IE L+  +++P  F CPISLE MQDPVT+C+GQTY+R++I  W S+G + TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 122 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQ-----------GRVLELLDSLKKVKG- 181
           D ++ PN TL +LI  W       +  RS  V+             V  LL     + G 
Sbjct: 65  DFTLIPNHTLRRLIQEW------CVANRSNGVERIPTPKQPADPTSVRALLSQASAITGT 124

Query: 182 ----QARVQSLKDLRHIVITHSSAKKMV-VDNGGVALISSLLGSFTSHAVGSEALGILVN 241
               ++R  +L+ LR         + ++   N    LI  L    TS  + SE+L +LV 
Sbjct: 125 HVSVRSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVM 184

Query: 242 LDLN--SELKKNLIQPTRISLIADILNEGSIETKINCTKFFKMLIVG---EDLKTEDVSS 301
           L +   ++       P R+  +  +L + SIET++N     +++  G    DLK    +S
Sbjct: 185 LPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNS 244

Query: 302 LSLLVGLLRMVKDQRHPNGA-TAGLSLLRTLCSNEPIRKQLVSIGA----VAKLGGTLSN 361
            S+  G+L ++++      A   G+  L  LCS +  R   ++ GA    + +L      
Sbjct: 245 ESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDR 304

Query: 362 LNTDCLESALYILDVLSSLPEGRLALKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFK 421
            +T   E AL  +++L   PEG  A      T+  LVK +++VS+  T++A   L A+  
Sbjct: 305 CDT---ERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC- 364

Query: 422 LAPEECALLAVEAGLAAKLLLVIQSGCNPELKQRSAELLKLCSLNYPDSIFISKSRTFG 453
            A E     A  AG+  +LLL++QS C    K+++ +LLKL   ++PD    + S  FG
Sbjct: 365 TAEERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPDYNSFANSDDFG 413

BLAST of Chy3G065860 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 151.0 bits (380), Expect = 2.3e-36
Identity = 121/408 (29.66%), Postives = 200/408 (49.02%), Query Frame = 0

Query: 62  IEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWD 121
           +E L+  +++P  F CPISL+ M DPVT+ +GQTY+R++I  W ++G+ TCP T   L D
Sbjct: 5   LEPLDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSD 64

Query: 122 DSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQG-----------RVLELLDSLKKVKG-- 181
            ++ PN TL +LI  W       +  RS  V+             V  LL     + G  
Sbjct: 65  FTLIPNHTLRRLIQEW------CVANRSNGVERIPTPKQPADPISVRSLLSQASAITGTH 124

Query: 182 ---QARVQSLKDLRHIVITHSSAKKMVVDNGGVALISSLL------GSFTSHAVGSEALG 241
              ++R  +++ LR +       + ++  +    ++  +L       S +S  V SE+L 
Sbjct: 125 VSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV-SESLA 184

Query: 242 ILVNLDL-NSELKKNLIQPTRISLIADILNEGSIETKINCTKFFKMLIVGE---DLKTED 301
           +LV L +  +E +     P+R+  +  +L + SIE ++N     +M++ G    DLK   
Sbjct: 185 LLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLII 244

Query: 302 VSSLSLLVGLLRMVKDQRHPNGA-TAGLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNL 361
             S S+  G+L ++K+      A   G+  +  LC  +  R   +S GA   L   L+  
Sbjct: 245 SGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAAD 304

Query: 362 NTDC-LESALYILDVLSSLPEGRLALKNCEDTIRNLVKLLMKVSEICTQFALSILWAVFK 421
              C  E  L  +++L  LPEG  A      T+  +VK +++VS+  T++A   L A+  
Sbjct: 305 FDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC- 364

Query: 422 LAPEECALLAVEAGLAAKLLLVIQSGCNPELKQRSAELLKLCSLNYPD 442
            A E C   A  AGL  +LLL++QS C    K+++  LLKL   ++PD
Sbjct: 365 TAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPD 404

BLAST of Chy3G065860 vs. TAIR 10
Match: AT5G64660.1 (CYS, MET, PRO, and GLY protein 2 )

HSP 1 Score: 146.0 bits (367), Expect = 7.5e-35
Identity = 119/398 (29.90%), Postives = 201/398 (50.50%), Query Frame = 0

Query: 69  VEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELWDDSVTPNR 128
           + VP+ F CPISL+ M+ PV+LC+G TY+R++I +W   G+ TCP TMQ L +    PNR
Sbjct: 8   ITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQNKDFIPNR 67

Query: 129 TLHQLIHTWF-SQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRHIVITHSS 188
           TL +LI  W  S +     + +E       E+ D++ +VK +   +  +++   ++    
Sbjct: 68  TLQRLIEIWSDSVRRRTCVESAELAAPTRDEIADAIDRVKIEKEERDDREVLSKIVRFGR 127

Query: 189 AKKMVVDNGG------------VALISSLLGSFTSHA---VGSEALGILVNLDLNSELKK 248
                 DN G            V LI+ +    TS A   V  EA+ IL  +      ++
Sbjct: 128 ESD---DNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIRSKVSDRR 187

Query: 249 ---NLIQPT---RISLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLL 308
              NLI      R+S+I  +   G++E KI+C    + + V  + K        L+  L+
Sbjct: 188 RFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAERDGLITELM 247

Query: 309 RMVKDQRHPNGATAGLSLLRTLCSNEPIRKQLVS---IGAVAK-LGGTLSNLNTDCLESA 368
           + +      +   + LS L  + S + ++  L+    IG V K L  + S+L+    E  
Sbjct: 248 KSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSSLSVSVTEKC 307

Query: 369 LYILDVLSSLPEGRLALKNCE-DTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAL 428
           L +L++L+S  EGR  +   + + ++ +VK LMKVS   T+ A+++LW+V  L  E+ AL
Sbjct: 308 LKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVSYLFKEDKAL 367

Query: 429 LAV-EAGLAAKLLLVIQSGCNPELKQRSAELLKLCSLN 439
            AV       K+LL++QS C+P +++   +LLK+  +N
Sbjct: 368 EAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVN 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q058P44.4e-14962.00U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1... [more]
Q9FHN91.1e-14761.50U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2... [more]
Q10PI92.7e-13053.48U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=T... [more]
Q9LT791.6e-3730.79U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA43.3e-3529.66U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A0A0LKY44.8e-24798.66RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G354100 PE... [more]
A0A5A7UYA82.2e-24497.77RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3BA202.2e-24497.77RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487822 PE=4 ... [more]
A0A6J1HFA18.2e-21587.75RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111462914... [more]
A0A6J1K7W02.6e-21387.31RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111492478 P... [more]
Match NameE-valueIdentityDescription
XP_031736908.13.97e-31398.66U-box domain-containing protein 30 isoform X2 [Cucumis sativus][more]
XP_011649559.19.46e-31398.66U-box domain-containing protein 30 isoform X1 [Cucumis sativus] >KAE8652062.1 hy... [more]
XP_008444531.11.27e-30997.77PREDICTED: U-box domain-containing protein 30-like [Cucumis melo] >KAA0060963.1 ... [more]
XP_038884660.12.32e-29995.10U-box domain-containing protein 31-like [Benincasa hispida][more]
XP_023536979.16.57e-27286.86U-box domain-containing protein 30-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G49810.13.1e-15062.00ARM repeat superfamily protein [more]
AT5G65920.17.7e-14961.50ARM repeat superfamily protein [more]
AT3G19380.11.1e-3830.79plant U-box 25 [more]
AT1G49780.12.3e-3629.66plant U-box 26 [more]
AT5G64660.17.5e-3529.90CYS, MET, PRO, and GLY protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 74..137
e-value: 2.1E-30
score: 117.0
IPR003613U box domainPFAMPF04564U-boxcoord: 71..141
e-value: 2.5E-16
score: 59.6
IPR003613U box domainPROSITEPS51698U_BOXcoord: 70..144
score: 40.196629
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 145..271
e-value: 8.5E-7
score: 29.8
coord: 272..445
e-value: 2.7E-11
score: 44.6
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 70..144
e-value: 6.1E-25
score: 89.1
NoneNo IPR availablePANTHERPTHR22849:SF142U-BOX DOMAIN-CONTAINING PROTEIN 30coord: 43..449
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 43..449
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 75..117
e-value: 1.35743E-24
score: 93.8374
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 67..141
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 160..441

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G065860.1Chy3G065860.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity