Chy3G064970 (gene) Cucumber (hystrix) v1

Overview
NameChy3G064970
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAlpha/beta-Hydrolases superfamily protein
LocationchrH03: 17482012 .. 17482992 (-)
RNA-Seq ExpressionChy3G064970
SyntenyChy3G064970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAATCCGGCGAACCAGAAACAGCCTTCGAGTTACTCCCGCTTATTCGTATCTACAAGAACGGAAGAATTGAGCGACTTGTCGGAATTGATTTCGTCCCACCGGGAACCGATCCTCTCACCGGCGTCACATCTAAAGACGTAACATTACTCCCGACATTTGGCGTCTCAGCGAGACTCTTCCTCCCAAACCTCACTCACTCAACTCAACGCCTTCCAGTAGTAGTCTATTTTCACGGTGGTTGCTTCTGCACACAATCACCTTTCACGGCCAAGTACCACAACTATCTTAACGCCCTTACTGCTGAAGCCAAAGTCGTGGCAGTTTCCGTTAACTACAGGAAGGCACCAGAGCATCCCATTCCCACAGCTTACGAAGACTCGTGGGCTACCCTTCAATGGGTCATTTCACACCGTGATGGGAAAGGACCTGAGATTTGGTTGAACAAACATGTAGATTTCAAAAGGGTGTTTTTGGCCGGAGCTAGTGCTGGTGCCAACATTGCTCACAACCTGGCAATGGTGGCCGGAGATCCTGATTGTGGGGTGAACATCAATCTCATTGGAGTTGCTTTGGAGCATCCATACTTTTGGGGCAGTGTGAGAATTGGTAAGGAGGCAGAGAATCCAGTGAAGGCGAGGTTATTTGATCAGCTGTGGGGGTTTATTTGTCCAGCGAGACCGGAGAACGATGATCCATGGGTGAATCCAGTGGCAGAAGGAGCAGGGAGGCTGGCGGGGCTGGGGAGTGGGAGGGTGCTAGTTTGTGTGGCGGAGAAAGATGTGCTGAGGGACAGAGGAAGGCTTTACTTTGAGGCGTTGGGGGGCAGTGGGTGGTTCGGGGTGGCGGAGATTGTAGAAACGGAGGATGAGGATCATATGTTTCATTTGAATGATTTGGAAGGTCAGAAGGCTAAGGATTTGATCCGACGCCTGGGGGATTTCTTTAACAGAGGCATGCCCCCTTCTCTGCTGCTTTGA

mRNA sequence

ATGGAATCCGGCGAACCAGAAACAGCCTTCGAGTTACTCCCGCTTATTCGTATCTACAAGAACGGAAGAATTGAGCGACTTGTCGGAATTGATTTCGTCCCACCGGGAACCGATCCTCTCACCGGCGTCACATCTAAAGACGTAACATTACTCCCGACATTTGGCGTCTCAGCGAGACTCTTCCTCCCAAACCTCACTCACTCAACTCAACGCCTTCCAGTAGTAGTCTATTTTCACGGTGGTTGCTTCTGCACACAATCACCTTTCACGGCCAAGTACCACAACTATCTTAACGCCCTTACTGCTGAAGCCAAAGTCGTGGCAGTTTCCGTTAACTACAGGAAGGCACCAGAGCATCCCATTCCCACAGCTTACGAAGACTCGTGGGCTACCCTTCAATGGGTCATTTCACACCGTGATGGGAAAGGACCTGAGATTTGGTTGAACAAACATGTAGATTTCAAAAGGGTGTTTTTGGCCGGAGCTAGTGCTGGTGCCAACATTGCTCACAACCTGGCAATGGTGGCCGGAGATCCTGATTGTGGGGTGAACATCAATCTCATTGGAGTTGCTTTGGAGCATCCATACTTTTGGGGCAGTGTGAGAATTGGTAAGGAGGCAGAGAATCCAGTGAAGGCGAGGTTATTTGATCAGCTGTGGGGGTTTATTTGTCCAGCGAGACCGGAGAACGATGATCCATGGGTGAATCCAGTGGCAGAAGGAGCAGGGAGGCTGGCGGGGCTGGGGAGTGGGAGGGTGCTAGTTTGTGTGGCGGAGAAAGATGTGCTGAGGGACAGAGGAAGGCTTTACTTTGAGGCGTTGGGGGGCAGTGGGTGGTTCGGGGTGGCGGAGATTGTAGAAACGGAGGATGAGGATCATATGTTTCATTTGAATGATTTGGAAGGTCAGAAGGCTAAGGATTTGATCCGACGCCTGGGGGATTTCTTTAACAGAGGCATGCCCCCTTCTCTGCTGCTTTGA

Coding sequence (CDS)

ATGGAATCCGGCGAACCAGAAACAGCCTTCGAGTTACTCCCGCTTATTCGTATCTACAAGAACGGAAGAATTGAGCGACTTGTCGGAATTGATTTCGTCCCACCGGGAACCGATCCTCTCACCGGCGTCACATCTAAAGACGTAACATTACTCCCGACATTTGGCGTCTCAGCGAGACTCTTCCTCCCAAACCTCACTCACTCAACTCAACGCCTTCCAGTAGTAGTCTATTTTCACGGTGGTTGCTTCTGCACACAATCACCTTTCACGGCCAAGTACCACAACTATCTTAACGCCCTTACTGCTGAAGCCAAAGTCGTGGCAGTTTCCGTTAACTACAGGAAGGCACCAGAGCATCCCATTCCCACAGCTTACGAAGACTCGTGGGCTACCCTTCAATGGGTCATTTCACACCGTGATGGGAAAGGACCTGAGATTTGGTTGAACAAACATGTAGATTTCAAAAGGGTGTTTTTGGCCGGAGCTAGTGCTGGTGCCAACATTGCTCACAACCTGGCAATGGTGGCCGGAGATCCTGATTGTGGGGTGAACATCAATCTCATTGGAGTTGCTTTGGAGCATCCATACTTTTGGGGCAGTGTGAGAATTGGTAAGGAGGCAGAGAATCCAGTGAAGGCGAGGTTATTTGATCAGCTGTGGGGGTTTATTTGTCCAGCGAGACCGGAGAACGATGATCCATGGGTGAATCCAGTGGCAGAAGGAGCAGGGAGGCTGGCGGGGCTGGGGAGTGGGAGGGTGCTAGTTTGTGTGGCGGAGAAAGATGTGCTGAGGGACAGAGGAAGGCTTTACTTTGAGGCGTTGGGGGGCAGTGGGTGGTTCGGGGTGGCGGAGATTGTAGAAACGGAGGATGAGGATCATATGTTTCATTTGAATGATTTGGAAGGTCAGAAGGCTAAGGATTTGATCCGACGCCTGGGGGATTTCTTTAACAGAGGCATGCCCCCTTCTCTGCTGCTTTGA

Protein sequence

MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARLFLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHPIPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPDCGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAEGAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDLEGQKAKDLIRRLGDFFNRGMPPSLLL*
Homology
BLAST of Chy3G064970 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 4.4e-82
Identity = 140/318 (44.03%), Postives = 207/318 (65.09%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MES + + + ELLP + ++ +G +ERL G +  PPG DP+TGV SKD+ + P  G+SAR+
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           + P      Q++P+++YFHGG F   S     YH  LN +  +A V+AVSVNYR APEHP
Sbjct: 61  YRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHP 120

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           +PTAYEDSW  L+ + +       E W+N + D   +FL G SAGANI+H+LA  A   D
Sbjct: 121 LPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD 180

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
             + I   G+ + HPYFWG+  IG E ++  + ++ D  W F+CP+   +DDPW+NP A+
Sbjct: 181 QTLKIK--GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFAD 240

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           G+  L GLG  RV++ VAEKD+L +RG++Y+E L  S W G  EI+ET+++DH+FH+ + 
Sbjct: 241 GSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEP 300

Query: 301 EGQKAKDLIRRLGDFFNR 319
           +  +A +++R L  F N+
Sbjct: 301 DCDEAMEMVRCLALFINQ 311

BLAST of Chy3G064970 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 4.3e-77
Identity = 146/323 (45.20%), Postives = 200/323 (61.92%), Query Frame = 0

Query: 5   EPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARLFLPN 64
           + E A +  P++ IYK+GRIERLVG   VPP ++P  GV SKDV   P   +S R++LP 
Sbjct: 2   DSEIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPE 61

Query: 65  L-----THSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEH 124
                 T ++ +LP++VYFHGG F  ++ F+  YH +L A  + +  VAVSV+YR+APEH
Sbjct: 62  KAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEH 121

Query: 125 PIPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAG-- 184
           PIPT+Y+DSW  L+WV SH  G G E WLNKH DF +VFLAG SAGANI H++ M A   
Sbjct: 122 PIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKD 181

Query: 185 --DPDCGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLF-DQLWGFICP-ARPENDDP 244
              P+      + G+ L HPYFW    +  +    V  R + + +W    P ++  +DDP
Sbjct: 182 KLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDP 241

Query: 245 WVNPVAEGAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFG-VAEIVETEDED 304
           ++N V   +  L+GLG G+VLV VAEKD L  +G  Y+E LG S W G V ++VET+ E 
Sbjct: 242 FINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEG 301

Query: 305 HMFHLNDLEGQKAKDLIRRLGDF 316
           H+FHL D   +KA +L+ R   F
Sbjct: 302 HVFHLRDPNSEKAHELVHRFAGF 324

BLAST of Chy3G064970 vs. ExPASy Swiss-Prot
Match: Q5NUF3 (2-hydroxyisoflavanone dehydratase OS=Glycine max OX=3847 GN=HIDH PE=1 SV=1)

HSP 1 Score: 287.7 bits (735), Expect = 1.6e-76
Identity = 139/314 (44.27%), Postives = 190/314 (60.51%), Query Frame = 0

Query: 7   ETAFELLPLIRIYKNGRIERLVGIDFVPPG-TDPLTGVTSKDVTLLPTFGVSARLFLPNL 66
           E   ELLPLIR+YK+G +ERL+  + V     DP TGV+SKD+ +     VSAR+FLP  
Sbjct: 4   EIVKELLPLIRVYKDGSVERLLSSENVAASPEDPQTGVSSKDIVIADNPYVSARIFLPKS 63

Query: 67  THSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHPIPTAY 126
            H+  +LP+ +YFHGG FC +S F+   H YLN L +EA ++A+SV++R  P HPIP AY
Sbjct: 64  HHTNNKLPIFLYFHGGAFCVESAFSFFVHRYLNILASEANIIAISVDFRLLPHHPIPAAY 123

Query: 127 EDSWATLQWVISHRDG---KGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPDCG 186
           ED W TL+W+ SH +      PE WL  H DF +V++ G ++GANIAHNL + AG+    
Sbjct: 124 EDGWTTLKWIASHANNTNTTNPEPWLLNHADFTKVYVGGETSGANIAHNLLLRAGNESLP 183

Query: 187 VNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPEN-DDPWVNPVAEG 246
            ++ ++G  L  P+FWGS  IG EA    +  L  ++W F CP  P   D+PW+NP   G
Sbjct: 184 GDLKILGGLLCCPFFWGSKPIGSEAVEGHEQSLAMKVWNFACPDAPGGIDNPWINPCVPG 243

Query: 247 AGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDLE 306
           A  LA L   ++LV +  KD  RDR  LY   +  SGW G  ++ +  DE+H F L   E
Sbjct: 244 APSLATLACSKLLVTITGKDEFRDRDILYHHTVEQSGWQGELQLFDAGDEEHAFQLFKPE 303

Query: 307 GQKAKDLIRRLGDF 316
              AK +I+RL  F
Sbjct: 304 THLAKAMIKRLASF 317

BLAST of Chy3G064970 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 7.5e-74
Identity = 138/317 (43.53%), Postives = 194/317 (61.20%), Query Frame = 0

Query: 11  ELLPLIRIYKNGRIERLVGIDFVPPG-TDPLTGVTSKDVTLLPTFGVSARLFLPNLTHST 70
           EL PL+R+YK+G +ER +G  FVPP   DP TGV++KD+ +     +SAR++LP L ++T
Sbjct: 16  ELPPLLRVYKDGTVERFLGSSFVPPSPEDPETGVSTKDIVISENPTISARVYLPKLNNTT 75

Query: 71  QRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHPIPTAYEDSW 130
           ++LP++VY+HGG FC +S F+  +  YLN + ++A V+ VS+ YR APEHP+P AYED W
Sbjct: 76  EKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPEHPLPAAYEDGW 135

Query: 131 ATLQWVISHRDGK----GPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPDCGVNI 190
             L+WV SH          + WL KH DF R ++ G ++GANIAHN A+  G       +
Sbjct: 136 YALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNAALRVGAEALPGGL 195

Query: 191 NLIGVALEHPYFWGSVRI------GKEAENPVKARLFDQLWGFICPARPEN-DDPWVNPV 250
            + GV    P FWGS  +      G E  +P+      Q+W F+ P  P   D+P +NP+
Sbjct: 196 RIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPM------QVWNFVYPDAPGGIDNPLINPL 255

Query: 251 AEGAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLN 310
           A GA  LA LG  ++LV VA KD LRDRG  Y+EA+  SGW G  E+ + E E+H F + 
Sbjct: 256 APGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEGEEHCFQIY 315

Query: 311 DLEGQKAKDLIRRLGDF 316
             E + +KDLI R+  F
Sbjct: 316 HPETENSKDLIGRIASF 326

BLAST of Chy3G064970 vs. ExPASy Swiss-Prot
Match: Q9ZQ91 (Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 1.8e-72
Identity = 134/312 (42.95%), Postives = 192/312 (61.54%), Query Frame = 0

Query: 9   AFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARLFLPNLTHS 68
           AF+  P+ R+YK+GRIERL+G   VPP   P  GV SKD+   P   +S R++LP    +
Sbjct: 6   AFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKV-T 65

Query: 69  TQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHPIPTAYEDS 128
            ++LP+++YFHGG F  ++ F+  YH +L +  A A  +A+SVNYR+APE P+P  YEDS
Sbjct: 66  VKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPYEDS 125

Query: 129 WATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPDCGVNINLI 188
           W +L+WV++H  G GPE W+NKH DF +VFLAG SAG NI+H+L M A       ++ + 
Sbjct: 126 WDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSL-IS 185

Query: 189 GVALEHPYFWGSVRIGK-EAENPVKARLFDQLWGFICPARPEN-DDPWVNPVAEGAGRLA 248
           G+ L HPYFW    I + E  +  K +  +  W    P   +  DDPW+N V       +
Sbjct: 186 GIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP---S 245

Query: 249 GLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDLEGQKAK 308
           GLG GRVLV VA  D+   +G  Y E L  SGW G  E++ET++E H+FHL +     A+
Sbjct: 246 GLGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNAR 305

Query: 309 DLIRRLGDFFNR 319
            ++++L +F N+
Sbjct: 306 QVVKKLEEFINK 312

BLAST of Chy3G064970 vs. ExPASy TrEMBL
Match: A0A0A0LLA8 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G360060 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 4.1e-192
Identity = 321/326 (98.47%), Postives = 323/326 (99.08%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MESGEPETAFELLPLIRIYKNGRIERLVGIDFVP GTDPLTGVTSKDVTLLPTFGVSARL
Sbjct: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPSGTDPLTGVTSKDVTLLPTFGVSARL 60

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP
Sbjct: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           IPTAYEDSWA LQWVISHRDGKGPE+W+NKHVDFKRVFLAGASAGANIAHNLAMVAGDPD
Sbjct: 121 IPTAYEDSWAALQWVISHRDGKGPEMWMNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
           CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE
Sbjct: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL
Sbjct: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLLL 327
           EGQKAKDLIRRLGDFFNR MPPSLLL
Sbjct: 301 EGQKAKDLIRRLGDFFNRDMPPSLLL 326

BLAST of Chy3G064970 vs. ExPASy TrEMBL
Match: A0A5A7V0M5 (Putative carboxylesterase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00060 PE=4 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 5.6e-189
Identity = 316/326 (96.93%), Postives = 319/326 (97.85%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MESGEPETAFELLPLIRIYKNGRIERLV  DFVPPGTDPLTGVTSKDVT LPTFGVSARL
Sbjct: 1   MESGEPETAFELLPLIRIYKNGRIERLVDTDFVPPGTDPLTGVTSKDVTFLPTFGVSARL 60

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSV+YRKAPEHP
Sbjct: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVDYRKAPEHP 120

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           IPTAYEDSWA LQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMV GDPD
Sbjct: 121 IPTAYEDSWAALQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVVGDPD 180

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
           CGVNI+LIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE
Sbjct: 181 CGVNIDLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDH+FHLNDL
Sbjct: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHLFHLNDL 300

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLLL 327
           EGQKAKDLIR LGDFFNR MPPSLLL
Sbjct: 301 EGQKAKDLIRDLGDFFNRDMPPSLLL 326

BLAST of Chy3G064970 vs. ExPASy TrEMBL
Match: A0A1S3BBJ6 (probable carboxylesterase 2 OS=Cucumis melo OX=3656 GN=LOC103487910 PE=4 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 1.8e-187
Identity = 314/326 (96.32%), Postives = 317/326 (97.24%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MESGEPETAFELLPLIRIYKNGRIERLV  DFVPPGTDPLTGVTSKDVT LPTFGVSARL
Sbjct: 32  MESGEPETAFELLPLIRIYKNGRIERLVDTDFVPPGTDPLTGVTSKDVTFLPTFGVSARL 91

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSV+YRKAPEHP
Sbjct: 92  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVDYRKAPEHP 151

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           IPTAYEDSWA LQWVISH DGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMV GDPD
Sbjct: 152 IPTAYEDSWAALQWVISHLDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVVGDPD 211

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
           CGVNI+LIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE
Sbjct: 212 CGVNIDLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 271

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDH+FHLND 
Sbjct: 272 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHLFHLNDS 331

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLLL 327
           EGQKAKDLIR LGDFFNR MPPSLLL
Sbjct: 332 EGQKAKDLIRDLGDFFNRDMPPSLLL 357

BLAST of Chy3G064970 vs. ExPASy TrEMBL
Match: A0A6J1K6U8 (probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111491707 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.6e-170
Identity = 278/325 (85.54%), Postives = 302/325 (92.92%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MESGEPETAFELLPL+R+YKNGRIERL+G DFVPPGTDPLTGVTSKDVT+ P  GVSARL
Sbjct: 1   MESGEPETAFELLPLLRVYKNGRIERLIGTDFVPPGTDPLTGVTSKDVTIFPESGVSARL 60

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLPNLTHSTQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEA+V+AVSV+YRKAPEHP
Sbjct: 61  FLPNLTHSTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEARVLAVSVDYRKAPEHP 120

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           +PTAYEDSWA LQW ISH +GKGPE+WLNKH DF+R+FLAGASAGANIAHNLAM+ G+PD
Sbjct: 121 VPTAYEDSWAALQWAISHCEGKGPEVWLNKHADFRRLFLAGASAGANIAHNLAMLDGEPD 180

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
            G N+ L+GVALEHPYFWGSVR  KEAE+P++  LFD+LWGFICPARPENDDPWVNPVAE
Sbjct: 181 YGENMELVGVALEHPYFWGSVRTAKEAEDPLRTTLFDRLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GA RLAG+GSGRVLVCVAEKDVLRDRGR Y+EAL GSGW GVAEIVETEDEDH+FHLNDL
Sbjct: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGRHYYEALAGSGWLGVAEIVETEDEDHLFHLNDL 300

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLL 326
           EGQKAKDLIRRLG FFNR MP SLL
Sbjct: 301 EGQKAKDLIRRLGAFFNRDMPHSLL 325

BLAST of Chy3G064970 vs. ExPASy TrEMBL
Match: A0A6J1HDJ9 (probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111462621 PE=4 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.4e-168
Identity = 276/325 (84.92%), Postives = 299/325 (92.00%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           ME GEPETAFELLPL+R+YKNGRIERL+G DFVPPGTDPLT VTSKDVT+ P  GVSARL
Sbjct: 1   MEFGEPETAFELLPLLRVYKNGRIERLIGTDFVPPGTDPLTSVTSKDVTIFPESGVSARL 60

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLPNLTHSTQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEAKV+AVSVNYRKAPEHP
Sbjct: 61  FLPNLTHSTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVLAVSVNYRKAPEHP 120

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           IP AYEDSWA LQW ISH +GKGPE+WLNKH DF+R+FLAGASAGANIAHNLAM+AG+PD
Sbjct: 121 IPAAYEDSWAALQWAISHCEGKGPEVWLNKHADFRRLFLAGASAGANIAHNLAMLAGEPD 180

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
            G N+ L+GVALEHPYFWGS R  KEAE+ ++ RLF++LWGFICPARPENDDPWVNPVAE
Sbjct: 181 SGENMELVGVALEHPYFWGSARTAKEAEDSMRTRLFERLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GA RLAG+GSGRVLVCVAEKDVLRDRGR Y+EAL GSGW GVAEIVE+EDEDH+FHLNDL
Sbjct: 241 GAARLAGMGSGRVLVCVAEKDVLRDRGRHYYEALAGSGWLGVAEIVESEDEDHLFHLNDL 300

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLL 326
           EGQKAKDLIRRLG FFNR MP SLL
Sbjct: 301 EGQKAKDLIRRLGAFFNRDMPHSLL 325

BLAST of Chy3G064970 vs. NCBI nr
Match: KGN62538.1 (hypothetical protein Csa_021888 [Cucumis sativus])

HSP 1 Score: 676 bits (1745), Expect = 1.11e-245
Identity = 321/326 (98.47%), Postives = 323/326 (99.08%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MESGEPETAFELLPLIRIYKNGRIERLVGIDFVP GTDPLTGVTSKDVTLLPTFGVSARL
Sbjct: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPSGTDPLTGVTSKDVTLLPTFGVSARL 60

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP
Sbjct: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           IPTAYEDSWA LQWVISHRDGKGPE+W+NKHVDFKRVFLAGASAGANIAHNLAMVAGDPD
Sbjct: 121 IPTAYEDSWAALQWVISHRDGKGPEMWMNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
           CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE
Sbjct: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL
Sbjct: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLLL 326
           EGQKAKDLIRRLGDFFNR MPPSLLL
Sbjct: 301 EGQKAKDLIRRLGDFFNRDMPPSLLL 326

BLAST of Chy3G064970 vs. NCBI nr
Match: XP_011649590.2 (probable carboxylesterase 2 [Cucumis sativus])

HSP 1 Score: 676 bits (1745), Expect = 2.19e-245
Identity = 321/326 (98.47%), Postives = 323/326 (99.08%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MESGEPETAFELLPLIRIYKNGRIERLVGIDFVP GTDPLTGVTSKDVTLLPTFGVSARL
Sbjct: 19  MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPSGTDPLTGVTSKDVTLLPTFGVSARL 78

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP
Sbjct: 79  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 138

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           IPTAYEDSWA LQWVISHRDGKGPE+W+NKHVDFKRVFLAGASAGANIAHNLAMVAGDPD
Sbjct: 139 IPTAYEDSWAALQWVISHRDGKGPEMWMNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 198

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
           CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE
Sbjct: 199 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 258

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL
Sbjct: 259 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 318

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLLL 326
           EGQKAKDLIRRLGDFFNR MPPSLLL
Sbjct: 319 EGQKAKDLIRRLGDFFNRDMPPSLLL 344

BLAST of Chy3G064970 vs. NCBI nr
Match: KAA0060874.1 (putative carboxylesterase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 666 bits (1718), Expect = 1.46e-241
Identity = 316/326 (96.93%), Postives = 319/326 (97.85%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MESGEPETAFELLPLIRIYKNGRIERLV  DFVPPGTDPLTGVTSKDVT LPTFGVSARL
Sbjct: 1   MESGEPETAFELLPLIRIYKNGRIERLVDTDFVPPGTDPLTGVTSKDVTFLPTFGVSARL 60

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSV+YRKAPEHP
Sbjct: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVDYRKAPEHP 120

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           IPTAYEDSWA LQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMV GDPD
Sbjct: 121 IPTAYEDSWAALQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVVGDPD 180

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
           CGVNI+LIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE
Sbjct: 181 CGVNIDLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDH+FHLNDL
Sbjct: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHLFHLNDL 300

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLLL 326
           EGQKAKDLIR LGDFFNR MPPSLLL
Sbjct: 301 EGQKAKDLIRDLGDFFNRDMPPSLLL 326

BLAST of Chy3G064970 vs. NCBI nr
Match: XP_008444640.2 (PREDICTED: probable carboxylesterase 2 [Cucumis melo])

HSP 1 Score: 661 bits (1705), Expect = 4.46e-239
Identity = 314/326 (96.32%), Postives = 317/326 (97.24%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MESGEPETAFELLPLIRIYKNGRIERLV  DFVPPGTDPLTGVTSKDVT LPTFGVSARL
Sbjct: 32  MESGEPETAFELLPLIRIYKNGRIERLVDTDFVPPGTDPLTGVTSKDVTFLPTFGVSARL 91

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSV+YRKAPEHP
Sbjct: 92  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVDYRKAPEHP 151

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           IPTAYEDSWA LQWVISH DGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMV GDPD
Sbjct: 152 IPTAYEDSWAALQWVISHLDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVVGDPD 211

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
           CGVNI+LIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE
Sbjct: 212 CGVNIDLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 271

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDH+FHLND 
Sbjct: 272 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHLFHLNDS 331

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLLL 326
           EGQKAKDLIR LGDFFNR MPPSLLL
Sbjct: 332 EGQKAKDLIRDLGDFFNRDMPPSLLL 357

BLAST of Chy3G064970 vs. NCBI nr
Match: XP_038885275.1 (probable carboxylesterase 2 [Benincasa hispida])

HSP 1 Score: 630 bits (1625), Expect = 2.19e-227
Identity = 295/325 (90.77%), Postives = 307/325 (94.46%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MESG+PE AFELLPL+R+YKNGRIERLVG DFVPPGTDPLTGVTSKDV +LP FG+SARL
Sbjct: 1   MESGKPEVAFELLPLLRVYKNGRIERLVGTDFVPPGTDPLTGVTSKDVMILPEFGISARL 60

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           FLP LT STQRLP+VVYFHGGCFCTQSPFTAKYHNYLNALTAEA+VVAVSVNYRKAPEHP
Sbjct: 61  FLPILTRSTQRLPLVVYFHGGCFCTQSPFTAKYHNYLNALTAEARVVAVSVNYRKAPEHP 120

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           IP AYEDSWA LQWVISHRD KGPEIWLN+HVDF+RVFL GASAGANIAHNLAMVAGDPD
Sbjct: 121 IPAAYEDSWAALQWVISHRDEKGPEIWLNEHVDFRRVFLVGASAGANIAHNLAMVAGDPD 180

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
           CGVN+ LIGVALEHPYFWGSVRIGKEA NPVKARLFDQLWGFICP RPENDDPWVNPVAE
Sbjct: 181 CGVNLELIGVALEHPYFWGSVRIGKEAGNPVKARLFDQLWGFICPVRPENDDPWVNPVAE 240

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           GA RLAGLGSGRVLVCVAEKDVLRDRGRLYFEALG SGW GVAEI ETEDEDH+FHLNDL
Sbjct: 241 GAARLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGSSGWLGVAEIEETEDEDHLFHLNDL 300

Query: 301 EGQKAKDLIRRLGDFFNRGMPPSLL 325
           EGQKAKDLI+RLGDFFNR MPPSLL
Sbjct: 301 EGQKAKDLIQRLGDFFNRDMPPSLL 325

BLAST of Chy3G064970 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 306.2 bits (783), Expect = 3.1e-83
Identity = 140/318 (44.03%), Postives = 207/318 (65.09%), Query Frame = 0

Query: 1   MESGEPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARL 60
           MES + + + ELLP + ++ +G +ERL G +  PPG DP+TGV SKD+ + P  G+SAR+
Sbjct: 1   MESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARI 60

Query: 61  FLPNLTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHP 120
           + P      Q++P+++YFHGG F   S     YH  LN +  +A V+AVSVNYR APEHP
Sbjct: 61  YRPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHP 120

Query: 121 IPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPD 180
           +PTAYEDSW  L+ + +       E W+N + D   +FL G SAGANI+H+LA  A   D
Sbjct: 121 LPTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD 180

Query: 181 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLFDQLWGFICPARPENDDPWVNPVAE 240
             + I   G+ + HPYFWG+  IG E ++  + ++ D  W F+CP+   +DDPW+NP A+
Sbjct: 181 QTLKIK--GIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFAD 240

Query: 241 GAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDL 300
           G+  L GLG  RV++ VAEKD+L +RG++Y+E L  S W G  EI+ET+++DH+FH+ + 
Sbjct: 241 GSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEP 300

Query: 301 EGQKAKDLIRRLGDFFNR 319
           +  +A +++R L  F N+
Sbjct: 301 DCDEAMEMVRCLALFINQ 311

BLAST of Chy3G064970 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 289.7 bits (740), Expect = 3.0e-78
Identity = 146/323 (45.20%), Postives = 200/323 (61.92%), Query Frame = 0

Query: 5   EPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARLFLPN 64
           + E A +  P++ IYK+GRIERLVG   VPP ++P  GV SKDV   P   +S R++LP 
Sbjct: 2   DSEIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPE 61

Query: 65  L-----THSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEH 124
                 T ++ +LP++VYFHGG F  ++ F+  YH +L A  + +  VAVSV+YR+APEH
Sbjct: 62  KAATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEH 121

Query: 125 PIPTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAG-- 184
           PIPT+Y+DSW  L+WV SH  G G E WLNKH DF +VFLAG SAGANI H++ M A   
Sbjct: 122 PIPTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKD 181

Query: 185 --DPDCGVNINLIGVALEHPYFWGSVRIGKEAENPVKARLF-DQLWGFICP-ARPENDDP 244
              P+      + G+ L HPYFW    +  +    V  R + + +W    P ++  +DDP
Sbjct: 182 KLSPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDP 241

Query: 245 WVNPVAEGAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFG-VAEIVETEDED 304
           ++N V   +  L+GLG G+VLV VAEKD L  +G  Y+E LG S W G V ++VET+ E 
Sbjct: 242 FINVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEG 301

Query: 305 HMFHLNDLEGQKAKDLIRRLGDF 316
           H+FHL D   +KA +L+ R   F
Sbjct: 302 HVFHLRDPNSEKAHELVHRFAGF 324

BLAST of Chy3G064970 vs. TAIR 10
Match: AT2G03550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-73
Identity = 134/312 (42.95%), Postives = 192/312 (61.54%), Query Frame = 0

Query: 9   AFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARLFLPNLTHS 68
           AF+  P+ R+YK+GRIERL+G   VPP   P  GV SKD+   P   +S R++LP    +
Sbjct: 6   AFDRSPMFRVYKSGRIERLLGETTVPPSLTPQNGVVSKDIIHSPEKNLSLRIYLPEKV-T 65

Query: 69  TQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHPIPTAYEDS 128
            ++LP+++YFHGG F  ++ F+  YH +L +  A A  +A+SVNYR+APE P+P  YEDS
Sbjct: 66  VKKLPILIYFHGGGFIIETAFSPPYHTFLTSAVAAANCLAISVNYRRAPEFPVPIPYEDS 125

Query: 129 WATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPDCGVNINLI 188
           W +L+WV++H  G GPE W+NKH DF +VFLAG SAG NI+H+L M A       ++ + 
Sbjct: 126 WDSLKWVLTHITGTGPETWINKHGDFGKVFLAGDSAGGNISHHLTMRAKKEKLCDSL-IS 185

Query: 189 GVALEHPYFWGSVRIGK-EAENPVKARLFDQLWGFICPARPEN-DDPWVNPVAEGAGRLA 248
           G+ L HPYFW    I + E  +  K +  +  W    P   +  DDPW+N V       +
Sbjct: 186 GIILIHPYFWSKTPIDEFEVRDVGKTKGVEGSWRVASPNSKQGVDDPWLNVVGSDP---S 245

Query: 249 GLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLNDLEGQKAK 308
           GLG GRVLV VA  D+   +G  Y E L  SGW G  E++ET++E H+FHL +     A+
Sbjct: 246 GLGCGRVLVMVAGDDLFVRQGWCYAEKLKKSGWEGEVEVMETKNEGHVFHLKNPNSDNAR 305

Query: 309 DLIRRLGDFFNR 319
            ++++L +F N+
Sbjct: 306 QVVKKLEEFINK 312

BLAST of Chy3G064970 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 5.0e-73
Identity = 138/322 (42.86%), Postives = 189/322 (58.70%), Query Frame = 0

Query: 5   EPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARLFLPN 64
           + E A +  PL++IYK+GRIERL+G   VPP ++P  GV SKDV       +S R++LP 
Sbjct: 2   DSEIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPE 61

Query: 65  --LTHSTQRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHPIP 124
                +  +LP++VYFHGG F  ++ F+  YH +L    + +  VAVSV+YR+APEHPI 
Sbjct: 62  KAAAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPIS 121

Query: 125 TAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAMVAGDPDCG 184
             ++DSW  L+WV +H  G G E WLNKH DF RVFL+G SAGANI H++AM A      
Sbjct: 122 VPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLS 181

Query: 185 VNIN---LIGVALEHPYFWGSVRIG-KEAENPVKARLFDQLWGFICP-ARPENDDPWVNP 244
             +N   + G+ L HPYFW    I  K+ ++       +  W    P ++   DDP +N 
Sbjct: 182 PGLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNV 241

Query: 245 VAEGAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHL 304
           V   +  L+GLG G+VLV VAEKD L  +G  Y   L  SGW G  E+VE+E EDH+FHL
Sbjct: 242 VQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHL 301

Query: 305 NDLEGQKAKDLIRRLGDFFNRG 320
              E   A +++ +   F   G
Sbjct: 302 LKPECDNAIEVMHKFSGFIKGG 323

BLAST of Chy3G064970 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 270.0 bits (689), Expect = 2.5e-72
Identity = 141/317 (44.48%), Postives = 190/317 (59.94%), Query Frame = 0

Query: 5   EPETAFELLPLIRIYKNGRIERLVGIDFVPPGTDPLTGVTSKDVTLLPTFGVSARLFLP- 64
           + E AF+  P  RI+KNG IERLV   FVPP  +P  GV SKD    P   +S R++LP 
Sbjct: 2   DSEIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQ 61

Query: 65  NLTHST--QRLPVVVYFHGGCFCTQSPFTAKYHNYLNALTAEAKVVAVSVNYRKAPEHPI 124
           N  + T  +++P++VYFHGG F  ++ F+  YH +L +  +    +AVSV YR+APEHPI
Sbjct: 62  NSVYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPI 121

Query: 125 PTAYEDSWATLQWVISHRDGKGPEIWLNKHVDFKRVFLAGASAGANIAHNLAM-VAGDPD 184
           PT YEDSW  +QW+ +H    GPE WLNKH DF +VFLAG SAGANIAH++A+ V  +  
Sbjct: 122 PTLYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKL 181

Query: 185 CGVNINLIGVALEHPYFWGSVRIGKEAENPVKA-RLFDQLWGFICPARPEN-DDPWVNPV 244
              N  + G+ L HPYF     I    E  V+A R +++LW    P      +DPW+N V
Sbjct: 182 PPENFKISGMILFHPYFLSKALI---EEMEVEAMRYYERLWRIASPDSGNGVEDPWINVV 241

Query: 245 AEGAGRLAGLGSGRVLVCVAEKDVLRDRGRLYFEALGGSGWFGVAEIVETEDEDHMFHLN 304
                 L GLG  RVLV VA  DVL   G  Y   L  SGW G  +++ET++E H+FHL 
Sbjct: 242 GSD---LTGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLR 301

Query: 305 DLEGQKAKDLIRRLGDF 316
           D + + A+ ++R   +F
Sbjct: 302 DPDSENARRVLRNFAEF 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SX784.4e-8244.03Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
Q9SMM94.3e-7745.20Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1[more]
Q5NUF31.6e-7644.272-hydroxyisoflavanone dehydratase OS=Glycine max OX=3847 GN=HIDH PE=1 SV=1[more]
Q5NUF47.5e-7443.532-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Q9ZQ911.8e-7242.95Probable carboxylesterase 7 OS=Arabidopsis thaliana OX=3702 GN=CXE7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LLA84.1e-19298.47Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G3600... [more]
A0A5A7V0M55.6e-18996.93Putative carboxylesterase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A1S3BBJ61.8e-18796.32probable carboxylesterase 2 OS=Cucumis melo OX=3656 GN=LOC103487910 PE=4 SV=1[more]
A0A6J1K6U82.6e-17085.54probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111491707 PE=4 SV=... [more]
A0A6J1HDJ91.4e-16884.92probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111462621 PE=4 S... [more]
Match NameE-valueIdentityDescription
KGN62538.11.11e-24598.47hypothetical protein Csa_021888 [Cucumis sativus][more]
XP_011649590.22.19e-24598.47probable carboxylesterase 2 [Cucumis sativus][more]
KAA0060874.11.46e-24196.93putative carboxylesterase 2 [Cucumis melo var. makuwa][more]
XP_008444640.24.46e-23996.32PREDICTED: probable carboxylesterase 2 [Cucumis melo][more]
XP_038885275.12.19e-22790.77probable carboxylesterase 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G47480.13.1e-8344.03alpha/beta-Hydrolases superfamily protein [more]
AT3G48700.13.0e-7845.20carboxyesterase 13 [more]
AT2G03550.11.3e-7342.95alpha/beta-Hydrolases superfamily protein [more]
AT3G48690.15.0e-7342.86alpha/beta-Hydrolases superfamily protein [more]
AT1G19190.12.5e-7244.48alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 75..296
e-value: 3.4E-47
score: 161.0
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 16..317
e-value: 2.7E-69
score: 236.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 6..318
NoneNo IPR availablePANTHERPTHR23024:SF458CARBOXYLESTERASE 2-RELATEDcoord: 5..317
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 5..317

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G064970.1Chy3G064970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane
molecular_function GO:0016787 hydrolase activity