Chy3G064750 (gene) Cucumber (hystrix) v1

Overview
NameChy3G064750
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAllene oxide synthase
LocationchrH03: 17343298 .. 17344896 (+)
RNA-Seq ExpressionChy3G064750
SyntenyChy3G064750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCTCCATTGTCATTCCTTCTCTTCAACCTCACTTGCGATTCCCATCGTCGAAAGAAACGACTCAAAGATCTCGTTCTAGAGTTGGGTTTGTTTCCACACGTCCAATCGTCTACGCCGCCGACGGAGTTTCTTCATCGTCTTCTTCCTCTCTTCAAGTGCCGCAGCGGATTGTTTCGCCGCCGGAACCCATCAAGCTTCCTTTGAGGAAGATTCCTGGTGATTATGGGCCGCCGATGTTTGGGGCCTTGAAGGATAGACAAGATTATTTCTATAATCAGGGAAGGGAAGAGTATTTGAAATCTCGAATGCTCCGGTATGAATCTACTGTGTATAGAACTAATATGCCACCGGGTCCATTTATCACTTCTGATTCCCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGATCATTCTAAAGTTGAGAAGAAAGATCTCTTCACCGGAACTTACATGCCCGTAACAGAGCTCACCGGCGGTTACAGGGTGCTTTCTTATATTGACCCATCTGAGCCCGATCACGCTAAGCTTAAACAGTTGATTTTCTTTCTCCTCAAGCACCGCCGGGATAAAATTTTGCCGGAATTTCACTCTACTTTTTCTGAGCTATTCGAGACTCTGGAAAAGGATTTGGCTGCTTCTGGTAGAGCAGAGTACAATGCTACCGGTGAACAAGCGGCGTTTAATTTCTTGGCTCGGTCTCTTTTCGGCGCTGATCCGGTAGATTCCAAACTAGGTCGTGACGCCCCGAAATTGATAGCGAAATGGGTCTTGTTCCAGCTTGGCCCTGTTCTGAGTCTCGGCCTCCCCAAGGTCGTCGAGGAGCTTCTACTCCGCACGGTCCGGCTCCCCCCGGCGTTGATCAAAGCCGATTACCGGCGGTTGTACGAATTCTTTTACAAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGATTGGGAATTTCGAGGGAAGAAGCTTGTCACAACTTGCTATTCACAACTTGTTTTAATTCATTTGGAGGGATGAAGATCTTTTTCCCCAACATGATCAAATGGATCGGCCGAGCCGGAGTAAATCTCCACACCCGACTAGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGTGCCATGGAACAGATGCCGCTGATGAAATCAGTAGTGTACGAAGCGTTCAGAATCGAGCCACCGGTTCCGGTTCAGTACGGTCGAGCGAAGAAAGACCTTGTGGTCGAAAGCCACGACGCGGCTTTCGAGATCAAAGAAGGAGAAGTGATCTGTGGGTATCAACCATTCGCAACAAGAGATCCAAAAATCTTCGACAGAGCCGACGAGTTCGTACCTGATCGGTTCACAGGCGACGGTGAGGAGCTGCTAAAACACGTTCTATGGTCAAACGGACCGGAAACTCAATCACCGTCGGTTCAGAACAAGCAGTGCGCAGGAAAAGACTTCATCGTCTTCATCTCTCGGCTTATGGTGGTTGAACTTTTCCTCCGGTACGACTCCTTCGACATCGAAGCCTCAAACACTCCGTTAGGTGCCGCCGTCACCGTAACTTCCCTAAAGAAAGCAAGTTTCTGA

mRNA sequence

ATGTCCTCCATTGTCATTCCTTCTCTTCAACCTCACTTGCGATTCCCATCGTCGAAAGAAACGACTCAAAGATCTCGTTCTAGAGTTGGGTTTGTTTCCACACGTCCAATCGTCTACGCCGCCGACGGAGTTTCTTCATCGTCTTCTTCCTCTCTTCAAGTGCCGCAGCGGATTGTTTCGCCGCCGGAACCCATCAAGCTTCCTTTGAGGAAGATTCCTGGTGATTATGGGCCGCCGATGTTTGGGGCCTTGAAGGATAGACAAGATTATTTCTATAATCAGGGAAGGGAAGAGTATTTGAAATCTCGAATGCTCCGGTATGAATCTACTGTGTATAGAACTAATATGCCACCGGGTCCATTTATCACTTCTGATTCCCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGATCATTCTAAAGTTGAGAAGAAAGATCTCTTCACCGGAACTTACATGCCCGTAACAGAGCTCACCGGCGGTTACAGGGTGCTTTCTTATATTGACCCATCTGAGCCCGATCACGCTAAGCTTAAACAGTTGATTTTCTTTCTCCTCAAGCACCGCCGGGATAAAATTTTGCCGGAATTTCACTCTACTTTTTCTGAGCTATTCGAGACTCTGGAAAAGGATTTGGCTGCTTCTGGTAGAGCAGAGTACAATGCTACCGGTGAACAAGCGGCGTTTAATTTCTTGGCTCGGTCTCTTTTCGGCGCTGATCCGGTAGATTCCAAACTAGGTCGTGACGCCCCGAAATTGATAGCGAAATGGGTCTTGTTCCAGCTTGGCCCTGTTCTGAGTCTCGGCCTCCCCAAGGTCGTCGAGGAGCTTCTACTCCGCACGGTCCGGCTCCCCCCGGCGTTGATCAAAGCCGATTACCGGCGGTTGTACGAATTCTTTTACAAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGATTGGGAATTTCGAGGGAAGAAGCTTGTCACAACTTGCTATTCACAACTTGTTTTAATTCATTTGGAGGGATGAAGATCTTTTTCCCCAACATGATCAAATGGATCGGCCGAGCCGGAGTAAATCTCCACACCCGACTAGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGTGCCATGGAACAGATGCCGCTGATGAAATCAGTAGTGTACGAAGCGTTCAGAATCGAGCCACCGGTTCCGGTTCAGTACGGTCGAGCGAAGAAAGACCTTGTGGTCGAAAGCCACGACGCGGCTTTCGAGATCAAAGAAGGAGAAGTGATCTGTGGGTATCAACCATTCGCAACAAGAGATCCAAAAATCTTCGACAGAGCCGACGAGTTCGTACCTGATCGGTTCACAGGCGACGGTGAGGAGCTGCTAAAACACGTTCTATGGTCAAACGGACCGGAAACTCAATCACCGTCGGTTCAGAACAAGCAGTGCGCAGGAAAAGACTTCATCGTCTTCATCTCTCGGCTTATGGTGGTTGAACTTTTCCTCCGGTACGACTCCTTCGACATCGAAGCCTCAAACACTCCGTTAGGTGCCGCCGTCACCGTAACTTCCCTAAAGAAAGCAAGTTTCTGA

Coding sequence (CDS)

ATGTCCTCCATTGTCATTCCTTCTCTTCAACCTCACTTGCGATTCCCATCGTCGAAAGAAACGACTCAAAGATCTCGTTCTAGAGTTGGGTTTGTTTCCACACGTCCAATCGTCTACGCCGCCGACGGAGTTTCTTCATCGTCTTCTTCCTCTCTTCAAGTGCCGCAGCGGATTGTTTCGCCGCCGGAACCCATCAAGCTTCCTTTGAGGAAGATTCCTGGTGATTATGGGCCGCCGATGTTTGGGGCCTTGAAGGATAGACAAGATTATTTCTATAATCAGGGAAGGGAAGAGTATTTGAAATCTCGAATGCTCCGGTATGAATCTACTGTGTATAGAACTAATATGCCACCGGGTCCATTTATCACTTCTGATTCCCGAGTTGTTGTTTTACTCGACGGGAAGAGTTTTCCTGTTCTTTTCGATCATTCTAAAGTTGAGAAGAAAGATCTCTTCACCGGAACTTACATGCCCGTAACAGAGCTCACCGGCGGTTACAGGGTGCTTTCTTATATTGACCCATCTGAGCCCGATCACGCTAAGCTTAAACAGTTGATTTTCTTTCTCCTCAAGCACCGCCGGGATAAAATTTTGCCGGAATTTCACTCTACTTTTTCTGAGCTATTCGAGACTCTGGAAAAGGATTTGGCTGCTTCTGGTAGAGCAGAGTACAATGCTACCGGTGAACAAGCGGCGTTTAATTTCTTGGCTCGGTCTCTTTTCGGCGCTGATCCGGTAGATTCCAAACTAGGTCGTGACGCCCCGAAATTGATAGCGAAATGGGTCTTGTTCCAGCTTGGCCCTGTTCTGAGTCTCGGCCTCCCCAAGGTCGTCGAGGAGCTTCTACTCCGCACGGTCCGGCTCCCCCCGGCGTTGATCAAAGCCGATTACCGGCGGTTGTACGAATTCTTTTACAAGTCGTCGGAGGCGGTGTTTGAGGAGGCGGATAGATTGGGAATTTCGAGGGAAGAAGCTTGTCACAACTTGCTATTCACAACTTGTTTTAATTCATTTGGAGGGATGAAGATCTTTTTCCCCAACATGATCAAATGGATCGGCCGAGCCGGAGTAAATCTCCACACCCGACTAGCACGGGAGATTCGTACTGCCGTAAAAGCCAACGGCGGGAAAATCACGATGGGTGCCATGGAACAGATGCCGCTGATGAAATCAGTAGTGTACGAAGCGTTCAGAATCGAGCCACCGGTTCCGGTTCAGTACGGTCGAGCGAAGAAAGACCTTGTGGTCGAAAGCCACGACGCGGCTTTCGAGATCAAAGAAGGAGAAGTGATCTGTGGGTATCAACCATTCGCAACAAGAGATCCAAAAATCTTCGACAGAGCCGACGAGTTCGTACCTGATCGGTTCACAGGCGACGGTGAGGAGCTGCTAAAACACGTTCTATGGTCAAACGGACCGGAAACTCAATCACCGTCGGTTCAGAACAAGCAGTGCGCAGGAAAAGACTTCATCGTCTTCATCTCTCGGCTTATGGTGGTTGAACTTTTCCTCCGGTACGACTCCTTCGACATCGAAGCCTCAAACACTCCGTTAGGTGCCGCCGTCACCGTAACTTCCCTAAAGAAAGCAAGTTTCTGA

Protein sequence

MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVSPPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSLFGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF*
Homology
BLAST of Chy3G064750 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.6e-209
Identity = 350/533 (65.67%), Postives = 442/533 (82.93%), Query Frame = 0

Query: 2   SSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVSP 61
           +S+ +PSL+  L+FPS   ++ R  S    VS RPI  +   +    SS  Q P    SP
Sbjct: 4   TSLSLPSLK--LQFPSHTSSSSRKNSSSYRVSIRPIQASVSEIPPYISSPSQSPSSSSSP 63

Query: 62  P-EPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 121
           P +  KLP +K+PGDYG P+ G  KDR DYFYNQG+ E+ KSR+ +++STV+RTNMPPGP
Sbjct: 64  PVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRTNMPPGP 123

Query: 122 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 181
           FI+ +  VVVLLDGKSFPVLFD SKVEKKDLFTGT+MP T+LTGGYRVLSY+DPSEP+HA
Sbjct: 124 FISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDPSEPNHA 183

Query: 182 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSL 241
           KLK+L+F+LL  RR++++PEFH+++SELFETLE +L+  G+A  NA  +QAAFNFLARSL
Sbjct: 184 KLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGKAGLNAANDQAAFNFLARSL 243

Query: 242 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 301
           +G +P D++LG D PKLI KWVLFQL P+L LGLPKV+E+L++ T RLPPAL+K DY+RL
Sbjct: 244 YGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPKVLEDLVMHTFRLPPALVKKDYQRL 303

Query: 302 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 361
           Y FFY++S +V +EA+++GISREEACHNLLF TCFNSFGG+KIFFPNM+KWIGRAG  LH
Sbjct: 304 YNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNMLKWIGRAGAKLH 363

Query: 362 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 421
           ++LA+EIR+ + +N GK+TM AME+MPLMKSVVYE+ RIEPPV  QYGRAK D+V+ESHD
Sbjct: 364 SQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGRAKHDMVIESHD 423

Query: 422 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPS 481
           A+FEIKEGE++ GYQPFAT+DPKIFDR++EFV DRF G +GE+LLKHVLWSNG ET++ S
Sbjct: 424 ASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVLWSNGSETENAS 483

Query: 482 VQNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 533
           + NKQCAGKDF+V +SRL++VELFLRYDSF+IE   +PLGAA+T+TSL++ASF
Sbjct: 484 INNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLRRASF 534

BLAST of Chy3G064750 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 2.5e-196
Identity = 332/529 (62.76%), Postives = 418/529 (79.02%), Query Frame = 0

Query: 3   SIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVSPP 62
           S+ +PSL  H + PS   T             RPI+     VS S  S++++ Q      
Sbjct: 8   SLPLPSL--HQKIPSKYST------------FRPII-----VSLSDKSTIEITQ------ 67

Query: 63  EPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 122
            PIKL  R IPGDYG P  G  KDR DYFYNQG+ ++ +SR+ +Y+ST++RTNMPPGPFI
Sbjct: 68  -PIKLSTRTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFI 127

Query: 123 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 182
           TS+ +V+VLLDGKSFPVLFD SKVEKKDLFTGT++P TELTGGYR+LSY+DPSEP+H KL
Sbjct: 128 TSNPKVIVLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKL 187

Query: 183 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSLFG 242
           K+L+FFLL  RRD ++PEFH T++ELFETL+K++   G   +N+  +QAAFNFLARSLFG
Sbjct: 188 KKLMFFLLSSRRDHVIPEFHETYTELFETLDKEMEEKGTVGFNSGSDQAAFNFLARSLFG 247

Query: 243 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYE 302
            +PV++KLG D P LI KW+L QL PV++LGLPK ++++LL T RLPP L+K DY+RLY+
Sbjct: 248 VNPVETKLGTDGPALIGKWILLQLHPVITLGLPKFLDDVLLHTFRLPPILVKKDYQRLYD 307

Query: 303 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 362
           FFY +S  +F EA++LGIS++EACHNLLF TCFNSFGGMKIFFPNM+K I +AGV +HTR
Sbjct: 308 FFYTNSANLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHTR 367

Query: 363 LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAA 422
           LA EIR+ VK+ GGKITM AME+MPLMKSVVYEA R++PPV  QYGRAK+DL +ESHDA 
Sbjct: 368 LANEIRSEVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDAV 427

Query: 423 FEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPSVQ 482
           FE+K+GE++ GYQPFAT+DPKIFDR  EFV DRF G +GE+LLKHVLWSNGPET+SP+V 
Sbjct: 428 FEVKKGEILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTVG 487

Query: 483 NKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKA 531
           NKQCAGKDF+V +SRL V E FLRY + +++   + LG+++T+TSLKKA
Sbjct: 488 NKQCAGKDFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

BLAST of Chy3G064750 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 1.8e-194
Identity = 328/531 (61.77%), Postives = 422/531 (79.47%), Query Frame = 0

Query: 8   SLQPHLRFPSSKET-TQRSRSRVGFVST-RPIVYAADGVSSSSSSSLQVPQRIVSPP--- 67
           ++ P+   PS K T    + S +  VS  RPI  +  G      S +++P     PP   
Sbjct: 11  AVNPNTLSPSPKSTPLPNTFSNLRRVSAFRPIKASLFG-----DSPIKIPGITSQPPPSS 70

Query: 68  EPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFI 127
           +   LP+R+IPGDYG P  G ++DR DYFYNQGREE+ KSR+ +Y+STVYR NMPPGPFI
Sbjct: 71  DETTLPIRQIPGDYGLPGIGPIQDRLDYFYNQGREEFFKSRLQKYKSTVYRANMPPGPFI 130

Query: 128 TSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKL 187
            S+ RV+VLLD KSFPVLFD SKVEKKDLFTGTYMP TELTGGYR+LSY+DPSEP+H KL
Sbjct: 131 ASNPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPNHTKL 190

Query: 188 KQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSLFG 247
           KQL+F L+K+RRD ++PEF S+F++L E +E DLA  G+A +N   EQAAFNFL+R+ FG
Sbjct: 191 KQLLFNLIKNRRDYVIPEFSSSFTDLCEVVEYDLATKGKAAFNDPAEQAAFNFLSRAFFG 250

Query: 248 ADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYE 307
             P+D+ LG+DAP LI+KWVLF L P+LS+GLPK VEE  L +VRLPP L++ DY RLYE
Sbjct: 251 VKPIDTPLGKDAPSLISKWVLFNLAPILSVGLPKEVEEATLHSVRLPPLLVQNDYHRLYE 310

Query: 308 FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTR 367
           FF  ++ +V +EA++ GISR+EACHN+LF  CFNS+GG KI FP+++KWIGRAG+ LHT+
Sbjct: 311 FFTSAAGSVLDEAEQSGISRDEACHNILFAVCFNSWGGFKILFPSLMKWIGRAGLELHTK 370

Query: 368 LAREIRTAVKA-NGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDA 427
           LA+EIR+A+++  GGK+TM AMEQMPLMKSVVYE  RIEPPV +QYG+AKKD ++ESH+A
Sbjct: 371 LAQEIRSAIQSTGGGKVTMAAMEQMPLMKSVVYETLRIEPPVALQYGKAKKDFILESHEA 430

Query: 428 AFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSVQ 487
           A+++KEGE++ GYQPFAT+DPKIFDR +EFV DRF G+G +L+++V+WSNGPET++PSV 
Sbjct: 431 AYQVKEGEMLFGYQPFATKDPKIFDRPEEFVADRFVGEGVKLMEYVMWSNGPETETPSVA 490

Query: 488 NKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 533
           NKQCAGKDF+V  +RL VVELF RYDSFDIE   + LGA++T+TSLK+++F
Sbjct: 491 NKQCAGKDFVVMAARLFVVELFKRYDSFDIEVGTSSLGASITLTSLKRSTF 536

BLAST of Chy3G064750 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 669.5 bits (1726), Expect = 3.2e-191
Identity = 326/512 (63.67%), Postives = 403/512 (78.71%), Query Frame = 0

Query: 22  TQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVSPPEPIKLPLRKIPGDYGPPMF 81
           T RS+     V TRPI       S S +  L V  R  S      LP+R IPG+YG P+ 
Sbjct: 16  TVRSKPLKFRVLTRPI-----KASGSETPDLTVATRTGSK----DLPIRNIPGNYGLPIV 75

Query: 82  GALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLF 141
           G +KDR DYFY+QG EE+ KSR+ +Y STVYR NMPPG FI  + +VV LLDGKSFPVLF
Sbjct: 76  GPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENPQVVALLDGKSFPVLF 135

Query: 142 DHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQLIFFLLKHRRDKILPEF 201
           D  KVEKKDLFTGTYMP TELTGGYR+LSY+DPSEP H KLK L+FFLLK  R++I PEF
Sbjct: 136 DVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFLLKSSRNRIFPEF 195

Query: 202 HSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSLFGADPVDSKLGRDAPKLIAKW 261
            +T+SELF++LEK+L+  G+A++  + +  AFNFLAR+ +G +P D+KL  DAP LI KW
Sbjct: 196 QATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPADTKLKADAPGLITKW 255

Query: 262 VLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYEFFYKSSEAVFEEADRLGIS 321
           VLF L P+LS+GLP+V+EE L+ T  LPPAL+K+DY+RLYEFF +S+  +  EAD+LGIS
Sbjct: 256 VLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEFFLESAGEILVEADKLGIS 315

Query: 322 REEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTRLAREIRTAVKANGGKITMG 381
           REEA HNLLF TCFN++GGMKI FPNM+K IGRAG  +H RLA EIR+ +K+NGG++TMG
Sbjct: 316 REEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRLAEEIRSVIKSNGGELTMG 375

Query: 382 AMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEVICGYQPFATRD 441
           A+E+M L KSVVYE  R EPPV  QYGRAKKDLV+ESHDAAF++K GE++ GYQP ATRD
Sbjct: 376 AIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAFKVKAGEMLYGYQPLATRD 435

Query: 442 PKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPSVQNKQCAGKDFIVFISRLMVV 501
           PKIFDRADEFVP+RF G +GE+LL+HVLWSNGPET++P+V NKQCAGKDF+V ++RL V+
Sbjct: 436 PKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGNKQCAGKDFVVLVARLFVI 495

Query: 502 ELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 533
           E+F RYDSFDIE   +PLG++V  +SL+KASF
Sbjct: 496 EIFRRYDSFDIEVGTSPLGSSVNFSSLRKASF 518

BLAST of Chy3G064750 vs. ExPASy Swiss-Prot
Match: Q40778 (Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 634.4 bits (1635), Expect = 1.1e-180
Identity = 295/471 (62.63%), Postives = 380/471 (80.68%), Query Frame = 0

Query: 63  EPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQ-GREEYLKSRMLRYESTVYRTNMPPGPF 122
           +P   PLR+IPG YG P F  +KDR +YFY   GR+EY +SRM +Y+STV+R NMPPGPF
Sbjct: 2   DPSSKPLREIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPGPF 61

Query: 123 ITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAK 182
           ++S+ +V+VLLD KSFP+LFD SKVEKKDLFTGTYMP T+LTGGYRVLSY+DPSEP HA+
Sbjct: 62  VSSNPKVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQ 121

Query: 183 LKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSLF 242
           LK L+FF+LK+  ++++P+F +T++ELFE LE +LA +G+A +N  GEQAAF FL R+ F
Sbjct: 122 LKNLLFFMLKNSSNRVIPQFETTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRAYF 181

Query: 243 GADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLY 302
            ++P ++KLG  AP LI+ WVLF L P L LGLP  ++E LL T RLP  LIK+ Y +LY
Sbjct: 182 NSNPEETKLGTSAPTLISSWVLFNLAPTLDLGLPWFLQEPLLHTFRLPAFLIKSTYNKLY 241

Query: 303 EFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHT 362
           ++F   +  V E+A++LG+ ++EA HN+LF  CFN+FGG+KI FPN +KWIG AG NLHT
Sbjct: 242 DYFQSVATPVMEQAEKLGVPKDEAVHNILFAVCFNTFGGVKILFPNTLKWIGLAGENLHT 301

Query: 363 RLAREIRTAVKANG-GKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 422
           +LA EIR A+K+ G G +T+ A+EQMPL KSVVYE+ RIEPPVP QYG+AK +  +ESHD
Sbjct: 302 QLAEEIRGAIKSYGDGNVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIESHD 361

Query: 423 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 482
           A FE+K+GE++ GYQPFAT+DPK+FDR +E+VPDRF GDGE LLK+V WSNGPET+SP+V
Sbjct: 362 ATFEVKKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGDGEALLKYVWWSNGPETESPTV 421

Query: 483 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKAS 532
           +NKQCAGKDF+V I+RL V+ELF RYDSF+IE   +PLGAAVT+T LK+AS
Sbjct: 422 ENKQCAGKDFVVLITRLFVIELFRRYDSFEIELGESPLGAAVTLTFLKRAS 472

BLAST of Chy3G064750 vs. ExPASy TrEMBL
Match: U5YTX9 (Allene oxide synthase OS=Cucumis sativus OX=3659 GN=AOS PE=2 SV=1)

HSP 1 Score: 1048.5 bits (2710), Expect = 9.4e-303
Identity = 523/532 (98.31%), Postives = 529/532 (99.44%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVS 60
           MSSIVIPSLQPHLRFPSS+ET QRSRSRVGFVS RPIVYAADGVSSSSSSSL+VPQRIVS
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIVYAADGVSSSSSSSLRVPQRIVS 60

Query: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
           PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP
Sbjct: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180
           FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA
Sbjct: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180

Query: 181 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSL 240
           KLKQL+FFLLKHRRDKI+PEFHSTFSELFETLEKDLAASGRAEYNA GEQAAFNFLARSL
Sbjct: 181 KLKQLVFFLLKHRRDKIMPEFHSTFSELFETLEKDLAASGRAEYNAPGEQAAFNFLARSL 240

Query: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL
Sbjct: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300

Query: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360
           YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH
Sbjct: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360

Query: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           T+LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD
Sbjct: 361 TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           AAFEIKEGE+ICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV
Sbjct: 421 AAFEIKEGEMICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480

Query: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 533
           QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532

BLAST of Chy3G064750 vs. ExPASy TrEMBL
Match: A0A5A7VCB0 (Allene oxide synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G006100 PE=4 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 1.7e-299
Identity = 518/532 (97.37%), Postives = 527/532 (99.06%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVS 60
           MSSIVIPSLQPHLRFPSS+ET QRSRSRVGFVS RPI YAADGVSSSSSSSLQVPQRIVS
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPI-YAADGVSSSSSSSLQVPQRIVS 60

Query: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
           PPEP KLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGP
Sbjct: 61  PPEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180
           FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA
Sbjct: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180

Query: 181 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSL 240
           KLKQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAA+GRAEYNA+GEQAAFNFLARSL
Sbjct: 181 KLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSL 240

Query: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL
Sbjct: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300

Query: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360
           Y+FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH
Sbjct: 301 YDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360

Query: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD
Sbjct: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSPSV
Sbjct: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSV 480

Query: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 533
           QNKQCAGKDFIVFISRL+VVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

BLAST of Chy3G064750 vs. ExPASy TrEMBL
Match: A0A1S3BAD5 (allene oxide synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487935 PE=4 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 2.4e-298
Identity = 516/532 (96.99%), Postives = 525/532 (98.68%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVS 60
           MSSIVIPSLQPHLRFPSS+ET QRSRSRVGFVS RPI YA DGVSSSSSSSLQVPQRIVS
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPI-YATDGVSSSSSSSLQVPQRIVS 60

Query: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
           PPEP KLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGP
Sbjct: 61  PPEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180
           FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA
Sbjct: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180

Query: 181 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSL 240
           KLKQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAA+GRAEYNA+GEQAAFNFLARSL
Sbjct: 181 KLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSL 240

Query: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL
Sbjct: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300

Query: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360
           Y+FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH
Sbjct: 301 YDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360

Query: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHD
Sbjct: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHD 420

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSPSV
Sbjct: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSV 480

Query: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 533
           QNKQCAGKDFIVFISRL+VVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

BLAST of Chy3G064750 vs. ExPASy TrEMBL
Match: Q8L5M5 (Allene oxide synthase OS=Cucumis melo OX=3656 GN=AOS PE=2 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.1e-295
Identity = 515/538 (95.72%), Postives = 524/538 (97.40%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVS 60
           MSSIVIPSLQPHLRFPSS+ET QRSRSRVGFVS RPI YA DGVSSSSSSSLQVPQRIVS
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPI-YATDGVSSSSSSSLQVPQRIVS 60

Query: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
           PPEP KLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGP
Sbjct: 61  PPEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180
           FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA
Sbjct: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180

Query: 181 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSL 240
           KLKQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAA+GRAEYNA+GEQAAFNFLARSL
Sbjct: 181 KLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSL 240

Query: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL
Sbjct: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300

Query: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360
           Y+FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH
Sbjct: 301 YDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360

Query: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHD
Sbjct: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHD 420

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           AAFEIKEGEVICGYQPFATRDPKIFDRADE VPDRFTG+GEELLKHV+WSNGPETQSPSV
Sbjct: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADELVPDRFTGEGEELLKHVIWSNGPETQSPSV 480

Query: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLG------AAVTVTSLKKASF 533
           QNKQCAGKDFIVFISRL+VVELFLRYDSFDIEASNTPLG      AAVTVTSLKKASF
Sbjct: 481 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVSAAVTVTSLKKASF 537

BLAST of Chy3G064750 vs. ExPASy TrEMBL
Match: A0A6J1K6Z3 (allene oxide synthase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491740 PE=4 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 5.7e-276
Identity = 477/536 (88.99%), Postives = 508/536 (94.78%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSS----SSLQVPQ 60
           ++SI +P  QPHLRFPS K+   RSRS +GF S R ++YAADGVSSSSS    SS+Q+PQ
Sbjct: 3   VTSITLPCPQPHLRFPSQKKPL-RSRSNLGFPSRR-LIYAADGVSSSSSSSPPSSVQLPQ 62

Query: 61  RIVSPPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120
           RI   PEP KLPLRKIPGDYGPP+FGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM
Sbjct: 63  RIAPTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 122

Query: 121 PPGPFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSE 180
           PPGPFIT+DSRVVVLLDGKSFPVLFD++KVEKKDLFTGTYMP TELTGGYRVLSYIDPSE
Sbjct: 123 PPGPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSE 182

Query: 181 PDHAKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFL 240
           PDH KLKQLIFFLLKHRRDKILPEFHSTFSELF+TLEKDLAA GRAEYNA GEQAAFNFL
Sbjct: 183 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFL 242

Query: 241 ARSLFGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKAD 300
           ARSLFGA+PVD+KLGRDAPKLIAKW+LFQLGPVL LGLPKVVEELLLRTVRLPP+LIKAD
Sbjct: 243 ARSLFGANPVDTKLGRDAPKLIAKWILFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKAD 302

Query: 301 YRRLYEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 360
           YRRLY+FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG
Sbjct: 303 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 362

Query: 361 VNLHTRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVV 420
            NLHT+LAREIRTAV+ANGG+ITMGAMEQMPLMKSVV+EAFRIEPPVPVQYGRAKKDLVV
Sbjct: 363 ANLHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVHEAFRIEPPVPVQYGRAKKDLVV 422

Query: 421 ESHDAAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQ 480
           ESHDAAFEIKEGE+ICG+QPFATRDPKIFDRADEFVPDRFTGDGE LLKHVLWSNGPETQ
Sbjct: 423 ESHDAAFEIKEGEMICGFQPFATRDPKIFDRADEFVPDRFTGDGERLLKHVLWSNGPETQ 482

Query: 481 SPSVQNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 533
           SP+VQNKQCAGKDFIVFISRL+VVE FLRYDSFDIE +N+PLGAA+TVTSLKKASF
Sbjct: 483 SPTVQNKQCAGKDFIVFISRLLVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

BLAST of Chy3G064750 vs. NCBI nr
Match: NP_001274390.1 (allene oxide synthase [Cucumis sativus] >AGZ95026.1 allene oxide synthase [Cucumis sativus] >KGN62561.1 hypothetical protein Csa_021833 [Cucumis sativus])

HSP 1 Score: 1044 bits (2699), Expect = 0.0
Identity = 523/532 (98.31%), Postives = 529/532 (99.44%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVS 60
           MSSIVIPSLQPHLRFPSS+ET QRSRSRVGFVS RPIVYAADGVSSSSSSSL+VPQRIVS
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPIVYAADGVSSSSSSSLRVPQRIVS 60

Query: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
           PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP
Sbjct: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180
           FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA
Sbjct: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180

Query: 181 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSL 240
           KLKQL+FFLLKHRRDKI+PEFHSTFSELFETLEKDLAASGRAEYNA GEQAAFNFLARSL
Sbjct: 181 KLKQLVFFLLKHRRDKIMPEFHSTFSELFETLEKDLAASGRAEYNAPGEQAAFNFLARSL 240

Query: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL
Sbjct: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300

Query: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360
           YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH
Sbjct: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360

Query: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           T+LAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD
Sbjct: 361 TQLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           AAFEIKEGE+ICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV
Sbjct: 421 AAFEIKEGEMICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480

Query: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532
           QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532

BLAST of Chy3G064750 vs. NCBI nr
Match: KAA0065268.1 (allene oxide synthase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1033 bits (2671), Expect = 0.0
Identity = 518/532 (97.37%), Postives = 527/532 (99.06%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVS 60
           MSSIVIPSLQPHLRFPSS+ET QRSRSRVGFVS RPI YAADGVSSSSSSSLQVPQRIVS
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPI-YAADGVSSSSSSSLQVPQRIVS 60

Query: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
           PPEP KLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGP
Sbjct: 61  PPEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180
           FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA
Sbjct: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180

Query: 181 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSL 240
           KLKQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAA+GRAEYNA+GEQAAFNFLARSL
Sbjct: 181 KLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSL 240

Query: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL
Sbjct: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300

Query: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360
           Y+FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH
Sbjct: 301 YDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360

Query: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD
Sbjct: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTG+GEELLKHV+WSNGPETQSPSV
Sbjct: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGEGEELLKHVIWSNGPETQSPSV 480

Query: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532
           QNKQCAGKDFIVFISRL+VVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF
Sbjct: 481 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 531

BLAST of Chy3G064750 vs. NCBI nr
Match: NP_001315375.1 (allene oxide synthase 1, chloroplastic [Cucumis melo] >AAM66138.1 allene oxide synthase [Cucumis melo])

HSP 1 Score: 1020 bits (2638), Expect = 0.0
Identity = 515/538 (95.72%), Postives = 524/538 (97.40%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVS 60
           MSSIVIPSLQPHLRFPSS+ET QRSRSRVGFVS RPI YA DGVSSSSSSSLQVPQRIVS
Sbjct: 1   MSSIVIPSLQPHLRFPSSQETPQRSRSRVGFVSIRPI-YATDGVSSSSSSSLQVPQRIVS 60

Query: 61  PPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120
           PPEP KLPLRK+PGDYGPPMFGALKDR DYFYNQGREEYLKSRMLRYESTVYRTNMPPGP
Sbjct: 61  PPEPTKLPLRKVPGDYGPPMFGALKDRHDYFYNQGREEYLKSRMLRYESTVYRTNMPPGP 120

Query: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180
           FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA
Sbjct: 121 FITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHA 180

Query: 181 KLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSL 240
           KLKQLIFFLLKHRRDKI+PEFHSTFSELFETLEKDLAA+GRAEYNA+GEQAAFNFLARSL
Sbjct: 181 KLKQLIFFLLKHRRDKIMPEFHSTFSELFETLEKDLAAAGRAEYNASGEQAAFNFLARSL 240

Query: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300
           FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL
Sbjct: 241 FGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRL 300

Query: 301 YEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360
           Y+FFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH
Sbjct: 301 YDFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLH 360

Query: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHD 420
           TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEA RIEPPVPVQYGRAKKDLVVESHD
Sbjct: 361 TRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEALRIEPPVPVQYGRAKKDLVVESHD 420

Query: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPSV 480
           AAFEIKEGEVICGYQPFATRDPKIFDRADE VPDRFTG+GEELLKHV+WSNGPETQSPSV
Sbjct: 421 AAFEIKEGEVICGYQPFATRDPKIFDRADELVPDRFTGEGEELLKHVIWSNGPETQSPSV 480

Query: 481 QNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTV------TSLKKASF 532
           QNKQCAGKDFIVFISRL+VVELFLRYDSFDIEASNTPLGAAVTV      TSLKKASF
Sbjct: 481 QNKQCAGKDFIVFISRLLVVELFLRYDSFDIEASNTPLGAAVTVSAAVTVTSLKKASF 537

BLAST of Chy3G064750 vs. NCBI nr
Match: XP_038885277.1 (allene oxide synthase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 977 bits (2526), Expect = 0.0
Identity = 496/533 (93.06%), Postives = 512/533 (96.06%), Query Frame = 0

Query: 2   SSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSS--LQVPQRIV 61
           +SI IP LQP LRFP  +E   RSRSRVGFVS RPI YAAD VSSSSSSS  +QVPQR V
Sbjct: 4   TSITIPCLQPPLRFPY-QEPPPRSRSRVGFVSLRPI-YAADRVSSSSSSSSSVQVPQRTV 63

Query: 62  SPPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPG 121
           S PEP KLPLRKIPGDYGPP+FGALKDRQDYFYNQG+EEYLKSRMLRYESTVYRTNMPPG
Sbjct: 64  STPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGKEEYLKSRMLRYESTVYRTNMPPG 123

Query: 122 PFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDH 181
           PFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGG+RVLSYIDPSEPDH
Sbjct: 124 PFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGHRVLSYIDPSEPDH 183

Query: 182 AKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARS 241
           AKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAA+GRA+YNA GEQAAFNFLARS
Sbjct: 184 AKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAAAGRAQYNAPGEQAAFNFLARS 243

Query: 242 LFGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRR 301
           LFGA+PVDSKLGRDAPKLI KWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRR
Sbjct: 244 LFGANPVDSKLGRDAPKLIGKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRR 303

Query: 302 LYEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNL 361
           LY+FFY+SSEAVFEEADRLGISREEACHNLLF TCFNSFGGMKIFFPNMIKWIGRAGV L
Sbjct: 304 LYDFFYESSEAVFEEADRLGISREEACHNLLFATCFNSFGGMKIFFPNMIKWIGRAGVKL 363

Query: 362 HTRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESH 421
           HT+LAREIRTAVK+NGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESH
Sbjct: 364 HTQLAREIRTAVKSNGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESH 423

Query: 422 DAAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQSPS 481
           DAAFEIKEGEVICGYQPFATRDPKIFDRA EFVPDRFTGDGEELLKHVLWSNGPETQSP+
Sbjct: 424 DAAFEIKEGEVICGYQPFATRDPKIFDRAGEFVPDRFTGDGEELLKHVLWSNGPETQSPT 483

Query: 482 VQNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532
           VQNKQCAGKDFIVFISRL+VVELFLRYDSFDIEA+NTPLGAAVTVTSLKKASF
Sbjct: 484 VQNKQCAGKDFIVFISRLLVVELFLRYDSFDIEATNTPLGAAVTVTSLKKASF 534

BLAST of Chy3G064750 vs. NCBI nr
Match: XP_022996510.1 (allene oxide synthase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 955 bits (2468), Expect = 0.0
Identity = 477/536 (88.99%), Postives = 508/536 (94.78%), Query Frame = 0

Query: 1   MSSIVIPSLQPHLRFPSSKETTQRSRSRVGFVSTRPIVYAADGVSSSSSSS----LQVPQ 60
           ++SI +P  QPHLRFPS K+   RSRS +GF S R ++YAADGVSSSSSSS    +Q+PQ
Sbjct: 3   VTSITLPCPQPHLRFPSQKKPL-RSRSNLGFPSRR-LIYAADGVSSSSSSSPPSSVQLPQ 62

Query: 61  RIVSPPEPIKLPLRKIPGDYGPPMFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 120
           RI   PEP KLPLRKIPGDYGPP+FGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM
Sbjct: 63  RIAPTPEPTKLPLRKIPGDYGPPVFGALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNM 122

Query: 121 PPGPFITSDSRVVVLLDGKSFPVLFDHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSE 180
           PPGPFIT+DSRVVVLLDGKSFPVLFD++KVEKKDLFTGTYMP TELTGGYRVLSYIDPSE
Sbjct: 123 PPGPFITNDSRVVVLLDGKSFPVLFDNAKVEKKDLFTGTYMPYTELTGGYRVLSYIDPSE 182

Query: 181 PDHAKLKQLIFFLLKHRRDKILPEFHSTFSELFETLEKDLAASGRAEYNATGEQAAFNFL 240
           PDH KLKQLIFFLLKHRRDKILPEFHSTFSELF+TLEKDLAA GRAEYNA GEQAAFNFL
Sbjct: 183 PDHEKLKQLIFFLLKHRRDKILPEFHSTFSELFQTLEKDLAAVGRAEYNAPGEQAAFNFL 242

Query: 241 ARSLFGADPVDSKLGRDAPKLIAKWVLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKAD 300
           ARSLFGA+PVD+KLGRDAPKLIAKW+LFQLGPVL LGLPKVVEELLLRTVRLPP+LIKAD
Sbjct: 243 ARSLFGANPVDTKLGRDAPKLIAKWILFQLGPVLRLGLPKVVEELLLRTVRLPPSLIKAD 302

Query: 301 YRRLYEFFYKSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 360
           YRRLY+FFY+SSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG
Sbjct: 303 YRRLYDFFYQSSEAVFEEADRLGISREEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAG 362

Query: 361 VNLHTRLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVV 420
            NLHT+LAREIRTAV+ANGG+ITMGAMEQMPLMKSVV+EAFRIEPPVPVQYGRAKKDLVV
Sbjct: 363 ANLHTQLAREIRTAVQANGGRITMGAMEQMPLMKSVVHEAFRIEPPVPVQYGRAKKDLVV 422

Query: 421 ESHDAAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRFTGDGEELLKHVLWSNGPETQ 480
           ESHDAAFEIKEGE+ICG+QPFATRDPKIFDRADEFVPDRFTGDGE LLKHVLWSNGPETQ
Sbjct: 423 ESHDAAFEIKEGEMICGFQPFATRDPKIFDRADEFVPDRFTGDGERLLKHVLWSNGPETQ 482

Query: 481 SPSVQNKQCAGKDFIVFISRLMVVELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 532
           SP+VQNKQCAGKDFIVFISRL+VVE FLRYDSFDIE +N+PLGAA+TVTSLKKASF
Sbjct: 483 SPTVQNKQCAGKDFIVFISRLLVVEFFLRYDSFDIEVANSPLGAAITVTSLKKASF 536

BLAST of Chy3G064750 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 669.5 bits (1726), Expect = 2.3e-192
Identity = 326/512 (63.67%), Postives = 403/512 (78.71%), Query Frame = 0

Query: 22  TQRSRSRVGFVSTRPIVYAADGVSSSSSSSLQVPQRIVSPPEPIKLPLRKIPGDYGPPMF 81
           T RS+     V TRPI       S S +  L V  R  S      LP+R IPG+YG P+ 
Sbjct: 16  TVRSKPLKFRVLTRPI-----KASGSETPDLTVATRTGSK----DLPIRNIPGNYGLPIV 75

Query: 82  GALKDRQDYFYNQGREEYLKSRMLRYESTVYRTNMPPGPFITSDSRVVVLLDGKSFPVLF 141
           G +KDR DYFY+QG EE+ KSR+ +Y STVYR NMPPG FI  + +VV LLDGKSFPVLF
Sbjct: 76  GPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPGAFIAENPQVVALLDGKSFPVLF 135

Query: 142 DHSKVEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQLIFFLLKHRRDKILPEF 201
           D  KVEKKDLFTGTYMP TELTGGYR+LSY+DPSEP H KLK L+FFLLK  R++I PEF
Sbjct: 136 DVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNLLFFLLKSSRNRIFPEF 195

Query: 202 HSTFSELFETLEKDLAASGRAEYNATGEQAAFNFLARSLFGADPVDSKLGRDAPKLIAKW 261
            +T+SELF++LEK+L+  G+A++  + +  AFNFLAR+ +G +P D+KL  DAP LI KW
Sbjct: 196 QATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNPADTKLKADAPGLITKW 255

Query: 262 VLFQLGPVLSLGLPKVVEELLLRTVRLPPALIKADYRRLYEFFYKSSEAVFEEADRLGIS 321
           VLF L P+LS+GLP+V+EE L+ T  LPPAL+K+DY+RLYEFF +S+  +  EAD+LGIS
Sbjct: 256 VLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEFFLESAGEILVEADKLGIS 315

Query: 322 REEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTRLAREIRTAVKANGGKITMG 381
           REEA HNLLF TCFN++GGMKI FPNM+K IGRAG  +H RLA EIR+ +K+NGG++TMG
Sbjct: 316 REEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRLAEEIRSVIKSNGGELTMG 375

Query: 382 AMEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEVICGYQPFATRD 441
           A+E+M L KSVVYE  R EPPV  QYGRAKKDLV+ESHDAAF++K GE++ GYQP ATRD
Sbjct: 376 AIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAFKVKAGEMLYGYQPLATRD 435

Query: 442 PKIFDRADEFVPDRFTG-DGEELLKHVLWSNGPETQSPSVQNKQCAGKDFIVFISRLMVV 501
           PKIFDRADEFVP+RF G +GE+LL+HVLWSNGPET++P+V NKQCAGKDF+V ++RL V+
Sbjct: 436 PKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGNKQCAGKDFVVLVARLFVI 495

Query: 502 ELFLRYDSFDIEASNTPLGAAVTVTSLKKASF 533
           E+F RYDSFDIE   +PLG++V  +SL+KASF
Sbjct: 496 EIFRRYDSFDIEVGTSPLGSSVNFSSLRKASF 518

BLAST of Chy3G064750 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 282.7 bits (722), Expect = 6.0e-76
Identity = 146/367 (39.78%), Postives = 216/367 (58.86%), Query Frame = 0

Query: 146 VEKKDLFTGTYMPVTELTGGYRVLSYIDPSEPDHAKLKQLIFFLLKHRRDKILPEFHSTF 205
           V+K+D+  G + P     GG RV  Y+D +EP HAK+K      LK      L E  S  
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 206 SELFETLEKDLAASGRAEYNATGEQAAFNFLARSLFGAD-PVDSKLGRDAPKLIAKWVLF 265
           +  + T+E +++ +G A Y    ++  F+FL  SL G D  V   +  +  K I  W+  
Sbjct: 64  NIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTWLAL 123

Query: 266 QLGPVLSLG-LPKVVEELLLRTVRLPPALIKADYRRLYEFFYKSS-EAVFEEADRLGISR 325
           Q+ P   LG +P+ +EE+LL T   P  LI  +Y++LY F  +++ + +    +  G++R
Sbjct: 124 QVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFGLTR 183

Query: 326 EEACHNLLFTTCFNSFGGMKIFFPNMIKWIGRAGVNLHTRLAREIRTAVKANGGKITMGA 385
           +EA  NLLF   FN++GG  +F P++I  I      L  R+  E+R  V  +G  +    
Sbjct: 184 DEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVR-RVCGSGSDLNFKT 243

Query: 386 MEQMPLMKSVVYEAFRIEPPVPVQYGRAKKDLVVESHDAAFEIKEGEVICGYQPFATRDP 445
           + +M L+KSVVYE  R  PPVP+Q+ RA+KD  + SHDA FE+K+GE++CGYQP   RD 
Sbjct: 244 VNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMRDA 303

Query: 446 KIFDRADEFVPDRFTGD-GEELLKHVLWSNGPETQSPSVQNKQCAGKDFIVFISRLMVVE 505
            +FD  +EF PDR+ G+ G ELL ++ WSNGP+T +PS  NKQCA KD +   + L+V +
Sbjct: 304 NVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLVAD 363

Query: 506 LFLRYDS 509
           LFLRYD+
Sbjct: 364 LFLRYDT 369

BLAST of Chy3G064750 vs. TAIR 10
Match: AT3G53280.1 (cytochrome p450 71b5 )

HSP 1 Score: 55.1 bits (131), Expect = 2.0e-07
Identity = 33/96 (34.38%), Postives = 52/96 (54.17%), Query Frame = 0

Query: 362 RLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGR-AKKDLVVESHD 421
           +L  EIR A+  N  KIT   +E++  +K V+ EAFR+ PP P+   R    D+ ++   
Sbjct: 324 KLQEEIRAALGPNKEKITEEDLEKVEYLKMVIEEAFRLHPPAPLLLPRLTMSDINIQ--- 383

Query: 422 AAFEIKEGEVICGYQPFATRDPKIFDRADEFVPDRF 457
             + I +  +I        RDPK + + DEF+P+RF
Sbjct: 384 -GYSIPKNTMIQINTYTIGRDPKNWTKPDEFIPERF 415

BLAST of Chy3G064750 vs. TAIR 10
Match: AT1G11610.1 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 46.2 bits (108), Expect = 9.5e-05
Identity = 31/96 (32.29%), Postives = 51/96 (53.12%), Query Frame = 0

Query: 362 RLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGR-AKKDLVVESHD 421
           +L  EIR+ ++ +G  I    +E M  +K+V+ E FR+ PP+P+   R   +D+ V+ +D
Sbjct: 323 KLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFRVHPPLPLILPRLLTEDVKVKGYD 382

Query: 422 AAFEIKEGEVICGYQPFATRDPKIF-DRADEFVPDR 456
               I  G  +        RDP I+   A+EF P+R
Sbjct: 383 ----IAAGTEVLINAWSIHRDPAIWGPDAEEFKPER 414

BLAST of Chy3G064750 vs. TAIR 10
Match: AT1G11610.2 (cytochrome P450, family 71, subfamily A, polypeptide 18 )

HSP 1 Score: 46.2 bits (108), Expect = 9.5e-05
Identity = 31/96 (32.29%), Postives = 51/96 (53.12%), Query Frame = 0

Query: 362 RLAREIRTAVKANGGKITMGAMEQMPLMKSVVYEAFRIEPPVPVQYGR-AKKDLVVESHD 421
           +L  EIR+ ++ +G  I    +E M  +K+V+ E FR+ PP+P+   R   +D+ V+ +D
Sbjct: 323 KLQNEIRSTIRPHGSYIKEKEVENMRYLKAVIKEVFRVHPPLPLILPRLLTEDVKVKGYD 382

Query: 422 AAFEIKEGEVICGYQPFATRDPKIF-DRADEFVPDR 456
               I  G  +        RDP I+   A+EF P+R
Sbjct: 383 ----IAAGTEVLINAWSIHRDPAIWGPDAEEFKPER 414

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
K4BV522.6e-20965.67Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P... [more]
Q9LLB02.5e-19662.76Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P... [more]
P484171.8e-19461.77Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE... [more]
Q962423.2e-19163.67Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P... [more]
Q407781.1e-18062.63Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
U5YTX99.4e-30398.31Allene oxide synthase OS=Cucumis sativus OX=3659 GN=AOS PE=2 SV=1[more]
A0A5A7VCB01.7e-29997.37Allene oxide synthase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold... [more]
A0A1S3BAD52.4e-29896.99allene oxide synthase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487935 P... [more]
Q8L5M51.1e-29595.72Allene oxide synthase OS=Cucumis melo OX=3656 GN=AOS PE=2 SV=1[more]
A0A6J1K6Z35.7e-27688.99allene oxide synthase 1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
NP_001274390.10.098.31allene oxide synthase [Cucumis sativus] >AGZ95026.1 allene oxide synthase [Cucum... [more]
KAA0065268.10.097.37allene oxide synthase 1 [Cucumis melo var. makuwa][more]
NP_001315375.10.095.72allene oxide synthase 1, chloroplastic [Cucumis melo] >AAM66138.1 allene oxide s... [more]
XP_038885277.10.093.06allene oxide synthase 1, chloroplastic [Benincasa hispida][more]
XP_022996510.10.088.99allene oxide synthase 1, chloroplastic-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G42650.12.3e-19263.67allene oxide synthase [more]
AT4G15440.16.0e-7639.78hydroperoxide lyase 1 [more]
AT3G53280.12.0e-0734.38cytochrome p450 71b5 [more]
AT1G11610.19.5e-0532.29cytochrome P450, family 71, subfamily A, polypeptide 18 [more]
AT1G11610.29.5e-0532.29cytochrome P450, family 71, subfamily A, polypeptide 18 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 386..402
score: 31.0
coord: 439..457
score: 31.17
coord: 485..503
score: 20.85
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 63..532
e-value: 1.9E-117
score: 395.1
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 67..507
IPR001128Cytochrome P450PFAMPF00067p450coord: 361..459
e-value: 2.5E-14
score: 52.9
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 53..529
NoneNo IPR availablePANTHERPTHR24286:SF255ALLENE OXIDE SYNTHASE, CHLOROPLASTICcoord: 53..529

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G064750.1Chy3G064750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009695 jasmonic acid biosynthetic process
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen