Chy3G064210 (gene) Cucumber (hystrix) v1

Overview
NameChy3G064210
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionProteasome subunit alpha type
LocationchrH03: 17057689 .. 17061182 (+)
RNA-Seq ExpressionChy3G064210
SyntenyChy3G064210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTAGGAATCAGTACGATACCGATGTCACCACATGGAGTCCCGCCGGTAGGTTGTTTCAGGTTGAGTACGCCATGGAGGCCGTCAAGCAAGGCTCCGCCGCCATTGGCCTCCGATCCAACACCCACGTGGTTCTAGCCTGCGTTAATAAGTCTAACTCTGAACTATCTTCTCACCAGAAGAAGATCTTCAAAGTCGACGATCACATCGGCGTCGCCATTTCCGGCCTTACTGCTGATGGACGCGTTCTTTCTCGTTATATGAGATCTGAGTGTATCAATTACAGCTACACCTATGAATCTCCGCTTCCTGTTGGTCGTCTCGTAGTTCAACTCGCTGATAAGGCTCAGGTTTGTGTACGGAAGCCCTCTTTCTGTTTACAATTTGCTCCTGCCTTTTTGGTTTCTTATGCATTTCTTGAATTTTAGATGTCTCTGCTGTGGGTTTATATTTGTTTGGATTTATTTTGTTAGTGATGGGTTTGGTTCTGAATTTACCAATTGTTTTAGCTGGATAGTTAAAGGTGGAACTTTTCAGATTATTATGCTCTCTACTAAGCTGAGGATTATAATGTCAAATTTATCTTTAGTCCGAAACAGAGGTTAAGTAACATTTTAGAAATGGGGGAATATCGCCCTCCATACCCTCTAATTTTCAGCATTTTGAGTGTTTAAAGGCCTTCCGTTTGCCTATCTTATGTTGTTTGTATTTGTTCCACTGATTCTTAACCGCTGCCCAGTAGTGTTTCAGCTCTCCAAATTGTTTGATTATTCTAATTGTTGAATTGGTCCGAACCCACTTGGCTTTGCTGGTAATTTGTTCTTTCTAAATTATGGTGTTATGTAGTTCAATAGTTTAGATTTTAGTAAAATCCAGGAAGTTGATTAATTATTTAGGGTCACGACGTATGTCGTACCACTTCTTATGTGTCATTTGATGTATGAGAGATTAAAAATGCTTTTGGTGGTCATGGGGTGGGATAGCTCGCTATAATTTCTTCGGGATAGGAAAGCCTTTATGAAGTCATGAATGTTGAATGTTCTTATGTGCACATATATGTCATGTCATTGAAGGACTGAGTTGTGAACAAGAAATGTTTGCATCATCTTCGATCAATAACCTAAGGAGCTTTTTCTTTCATTGTATCTTATGGAAGTCCAGTTTCTTACCAAGAAAAGGGAGAGAGAGAGAGAGAGAAAGCTTAGGAATATTGTATTCCGAAAATTGGGTTGAATTTATATTAGCTTGTGATGGGGTAATGAGAGATAGGATGGGGTGTGGTATTCTCAACCTTGAATATTAACAAAAAGAACAATACGTTCTTGAATTTTGTTTCATTTTGGTCGTTCTGTGTTGGTGTTGGATTTTGTTTAAATGGATCCTTTATACGGGTTAGATTCTTCTATCAAGTTCTTTGGTTTTCCTAATCATTTTGATTTTCCTAATCATTTTGATTTCTCAATTCATTTTTGCTGCCCAACCCCTTGCTTCCCTTTCAATTTAGAGAATAAAATGTTGGTGAATGTGATGCAATACTTGCACTGCAGTGAAAATGAAGAAACAATCGGTAACCTGGTTTATTGCATTTATCTCACTTTTTGTTTTTATTAAACCATCAATTCACCCAAAAGCTTAAGCTGGTGGTTGAAGGCAAATTTAATTATATATCACCAACACTTCCCCTCTCTTGTGGGCTTGAAATATTTGAAAGACCCAAAAAGTGGAATCAATTTTACCTCACTCGTGTACTTGTGGGCTTGAAATATTGAAAGGCCCAATAAGTGGAAATTGATTTTAATTGGGGAGGAAACGGCAATGCAAGGGCTTGAACACAGGACCTCCCTGGACCTCTTACTCTGATACCATATTAAACCACTAATTCACCCAAAAGCTTGGTGGTTGATGGCAAATTTAATTATATATCACCAACCGTTTTTATAAAGTGTATTCAACTACGAAAATGAAGACAAAACGACCTAGAGTCTAGGGGTAGAGAAAATGCCATCTACAGCTAGCTAATACACGAAGGAACAAACTGGGATCTAAAATACTGCATGGTTATGTTATAGAACAACTTTTTGTGCATGGATTTACGAACAAGAGGCTTTATGAAGAACAAAATCACTGAAGTTATTTAAGGATTTCTCTGTAATAAAAGATTGTTTTGTTTCTAACCACATCAGTCTCAAAAAGCTCTAATGGCAAACTTCCAAAGGCTCCCACTGAACAAGCTTCAAGGATCCTATTTTCATGCATATGAGAAAGCAGTCCGTAAATTGCATTTATCCCCTCTTCAACCCCCTACTTTTTTTCTGTTGTGACTTAGCATATCAACAGATATGGGTAAAGGGAACTGGCCTGCCATTTTGGTGTACCTGTACCCTTTTGTTTCTGTGTCAATTGATTTGGCTACTGGTTTTGATTATGGTGAATTCTTCCCCTCAACAGTATTTTTAAGTGTTCAGGTTAGACCTTATGGCTCTGCAACATATGTAGTTGTTAATTGTCATTAATGTTTTGCTGACCCCTTCTCCTGCCATGAATCTGTTAGGAACGAGTATAGTATGAGATGCTTTTGTTCTGTAATGGTTAGAATGGGATGACCAATGTAGTACTGGATTGGCTTTCCCACCTCAATAACAGGCTTTTGGGGTGTGGTTAAGGTGCTTGAGTACCTAACAAAATCATGTAGTTGTCTGTCTTTAAGCTGTAGGTAGGATTCAGCCCAACCTTAGAAGCCAACCAAGTATAGTGAGTTTCTTATCATTTTCGACATTAAAGTTTATCCATCTTTTCTAAGATGAGCAAGAGCTCTTAGAGACAAATAATGCCTTAACAGGAATAAAACTGGATACTTTATTGAGGGTATATCCTCTTTGAACTGTTTTATTATACGTGTCCTTCTAGGACCCTAAGCATGGTTATTTTTATAGGGAAAACCACTTCTCATATTCATAACCTTTTGTTTCTCTCAAATTATCTTGGCCACATGTCACAGGTCTGCACTCAGCGCTCGTGGAAACGTCCATATGGTGTTGGACTCCTGGTAGGTGGTCTGGACGAGTCCGGCGCTCATCTTTACTATAACTGCCCAAGTGGCAATTACTTTGAGTATCAAGCTTTTGCCATAGGGTCCCGTTCACAGGCTGCAAAGACATACTTGGAAAGAAGGTTTGAGAACTTCAAGGACTCTTCTAGAGATGAGTTGATCAAAGATGCACTGATCGCTACCAGAGAAACCTTGCAAGGAGAAAAACTGACTTCCTCCATATGCACAATTGCCATTTTAGGAGTTGGGGAACCTTTTCATATATTGGATAGGGAAACTGTGCAGAAGTTGATTGATGAATTTGAGATTGTTAAGGAGGATGACGTTCCTGCTGTGGATCAGGATATCGCTCCTGAGCAGGGTGATTCTGCTGCCCCTGAACCAGCTGCAGGGACAGACCAAGGAAGTGCCCCAGATCAAGGTGTTGCGCCGATGGATATCTAA

mRNA sequence

ATGTTTAGGAATCAGTACGATACCGATGTCACCACATGGAGTCCCGCCGGTAGGTTGTTTCAGGTTGAGTACGCCATGGAGGCCGTCAAGCAAGGCTCCGCCGCCATTGGCCTCCGATCCAACACCCACGTGGTTCTAGCCTGCGTTAATAAGTCTAACTCTGAACTATCTTCTCACCAGAAGAAGATCTTCAAAGTCGACGATCACATCGGCGTCGCCATTTCCGGCCTTACTGCTGATGGACGCGTTCTTTCTCGTTATATGAGATCTGAGTGTATCAATTACAGCTACACCTATGAATCTCCGCTTCCTGTTGGTCGTCTCGTAGTTCAACTCGCTGATAAGGCTCAGTCTCAAAAAGCTCTAATGGCAAACTTCCAAAGGCTCCCACTGAACAAGCTTCAAGGATCCTATTTTCATGCATATGAGAAAGCAGTCCATATGGGTAAAGGGAACTGGCCTGCCATTTTGGTGTACCTGTACCCTTTTGTTTCTGTGTCAATTGATTTGGCTACTGGTTTTGATTATGGTGAATTCTTCCCCTCAACAGTATTTTTAAGTGTTCAGGTCTGCACTCAGCGCTCGTGGAAACGTCCATATGGTGTTGGACTCCTGGTAGGTGGTCTGGACGAGTCCGGCGCTCATCTTTACTATAACTGCCCAAGTGGCAATTACTTTGAGTATCAAGCTTTTGCCATAGGGTCCCGTTCACAGGCTGCAAAGACATACTTGGAAAGAAGGTTTGAGAACTTCAAGGACTCTTCTAGAGATGAGTTGATCAAAGATGCACTGATCGCTACCAGAGAAACCTTGCAAGGAGAAAAACTGACTTCCTCCATATGCACAATTGCCATTTTAGGAGTTGGGGAACCTTTTCATATATTGGATAGGGAAACTGTGCAGAAGTTGATTGATGAATTTGAGATTGTTAAGGAGGATGACGTTCCTGCTGTGGATCAGGATATCGCTCCTGAGCAGGGTGATTCTGCTGCCCCTGAACCAGCTGCAGGGACAGACCAAGGAAGTGCCCCAGATCAAGGTGTTGCGCCGATGGATATCTAA

Coding sequence (CDS)

ATGTTTAGGAATCAGTACGATACCGATGTCACCACATGGAGTCCCGCCGGTAGGTTGTTTCAGGTTGAGTACGCCATGGAGGCCGTCAAGCAAGGCTCCGCCGCCATTGGCCTCCGATCCAACACCCACGTGGTTCTAGCCTGCGTTAATAAGTCTAACTCTGAACTATCTTCTCACCAGAAGAAGATCTTCAAAGTCGACGATCACATCGGCGTCGCCATTTCCGGCCTTACTGCTGATGGACGCGTTCTTTCTCGTTATATGAGATCTGAGTGTATCAATTACAGCTACACCTATGAATCTCCGCTTCCTGTTGGTCGTCTCGTAGTTCAACTCGCTGATAAGGCTCAGTCTCAAAAAGCTCTAATGGCAAACTTCCAAAGGCTCCCACTGAACAAGCTTCAAGGATCCTATTTTCATGCATATGAGAAAGCAGTCCATATGGGTAAAGGGAACTGGCCTGCCATTTTGGTGTACCTGTACCCTTTTGTTTCTGTGTCAATTGATTTGGCTACTGGTTTTGATTATGGTGAATTCTTCCCCTCAACAGTATTTTTAAGTGTTCAGGTCTGCACTCAGCGCTCGTGGAAACGTCCATATGGTGTTGGACTCCTGGTAGGTGGTCTGGACGAGTCCGGCGCTCATCTTTACTATAACTGCCCAAGTGGCAATTACTTTGAGTATCAAGCTTTTGCCATAGGGTCCCGTTCACAGGCTGCAAAGACATACTTGGAAAGAAGGTTTGAGAACTTCAAGGACTCTTCTAGAGATGAGTTGATCAAAGATGCACTGATCGCTACCAGAGAAACCTTGCAAGGAGAAAAACTGACTTCCTCCATATGCACAATTGCCATTTTAGGAGTTGGGGAACCTTTTCATATATTGGATAGGGAAACTGTGCAGAAGTTGATTGATGAATTTGAGATTGTTAAGGAGGATGACGTTCCTGCTGTGGATCAGGATATCGCTCCTGAGCAGGGTGATTCTGCTGCCCCTGAACCAGCTGCAGGGACAGACCAAGGAAGTGCCCCAGATCAAGGTGTTGCGCCGATGGATATCTAA

Protein sequence

MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQKKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQKALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFFPSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI*
Homology
BLAST of Chy3G064210 vs. ExPASy Swiss-Prot
Match: O23712 (Proteasome subunit alpha type-1-B OS=Arabidopsis thaliana OX=3702 GN=PAF2 PE=1 SV=2)

HSP 1 Score: 409.5 bits (1051), Expect = 4.0e-113
Identity = 220/353 (62.32%), Postives = 250/353 (70.82%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS +HVVLACVNK+ SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           +KIFKVDDHIGVAI+GLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKA    
Sbjct: 61  RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLER+FE+F++SS+++LIKDA++A RETLQGE L SS+CT+++LGV EPFH LD+E++
Sbjct: 241 KTYLERKFESFQESSKEDLIKDAIMAIRETLQGETLKSSLCTVSVLGVDEPFHFLDQESI 277

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 354
           QK+ID FE V E++  A + +  PE    AAP  A   +QG + DQ VAPM+I
Sbjct: 301 QKVIDTFEKVPEEEEDAGEGEAEPE----AAPGAAGTGEQGGSGDQDVAPMEI 277

BLAST of Chy3G064210 vs. ExPASy Swiss-Prot
Match: P34066 (Proteasome subunit alpha type-1-A OS=Arabidopsis thaliana OX=3702 GN=PAF1 PE=1 SV=3)

HSP 1 Score: 403.7 bits (1036), Expect = 2.2e-111
Identity = 221/353 (62.61%), Postives = 243/353 (68.84%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS +HVVLACVNK+ SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           +KIFKVDDHIGVAI+GLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKA    
Sbjct: 61  RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFE+F DSSR++LIKDA++A RETLQGE L SS+CT+AILGV EPFH LD+E +
Sbjct: 241 KTYLERRFESFGDSSREDLIKDAILAVRETLQGETLKSSLCTVAILGVDEPFHFLDQEAI 278

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 354
           QK+ID FE V E++    +      +   AAP    G   G A DQ VAPM++
Sbjct: 301 QKVIDTFEKVPEEEEGEGEAGEGEAEAAEAAPAERGG---GVAGDQDVAPMEM 278

BLAST of Chy3G064210 vs. ExPASy Swiss-Prot
Match: P52428 (Proteasome subunit alpha type-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PAF1 PE=2 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 7.1e-94
Identity = 193/358 (53.91%), Postives = 226/358 (63.13%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSA +GLRS TH VLA  NK+ SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSACVGLRSRTHAVLAAANKAASELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           +K+F+V DH GVA++GLTADGRVLSR++RSECIN+++ Y++PLPV RL ++LADKA    
Sbjct: 61  RKVFRVADHAGVALAGLTADGRVLSRFLRSECINHAFVYDAPLPVSRLALRLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLV GLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVAGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAIL-----GVGEPFHIL 300
           KT+LERRFE + D + ++LIKDAL A +ETLQGEKLTSS CT+AI+     G  EPF ++
Sbjct: 241 KTFLERRFEGYNDYTPEQLIKDALSAIKETLQGEKLTSSNCTVAIVGRKDDGTVEPFEMI 270

Query: 301 DRETVQKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 354
           D + +Q++ID  E        A ++  A E   S+  E   GTD         APMDI
Sbjct: 301 DVKRIQEIIDSME--------AAEEAPAAEAESSSMQEEDKGTD--------AAPMDI 270

BLAST of Chy3G064210 vs. ExPASy Swiss-Prot
Match: Q27562 (Proteasome subunit alpha type-1 OS=Dictyostelium discoideum OX=44689 GN=psmA1 PE=3 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 6.5e-63
Identity = 132/310 (42.58%), Postives = 179/310 (57.74%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           M+RNQYD+D+TT+SP+GR+ Q+EYAMEAVKQG AA+ L+S    VL  + +S+SEL S+Q
Sbjct: 1   MYRNQYDSDITTFSPSGRIHQIEYAMEAVKQGGAAVALKSKQFAVLVSLKRSSSELGSYQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIF VDDH+G+AISGLTAD R+L  YMR+EC NY+Y YES + V RLV ++ADK     
Sbjct: 61  KKIFVVDDHVGIAISGLTADARMLCNYMRNECSNYNYVYESQMRVERLVTKVADKH---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QV TQR  +RPYGVGLLV G D+ GAH+Y  CPSGN+++Y++ +IGSRSQ+A
Sbjct: 181 --------QVHTQRYGRRPYGVGLLVAGYDQLGAHIYQTCPSGNFYDYKSISIGSRSQSA 238

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQG--EKLTSSICTIAILGVGEPFHILDRE 300
           KTYLE+ FE+F++ + ++LI   L A RET+    + L S   +I I+G G+PF I++  
Sbjct: 241 KTYLEKHFESFEECTLEQLITHGLRALRETIPNTDDTLNSKNVSIGIVGEGQPFKIIEEA 238

Query: 301 TVQKLIDEFE 309
             Q  ++  E
Sbjct: 301 DAQPYLNLLE 238

BLAST of Chy3G064210 vs. ExPASy Swiss-Prot
Match: Q9R1P4 (Proteasome subunit alpha type-1 OS=Mus musculus OX=10090 GN=Psma1 PE=1 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.1e-58
Identity = 132/341 (38.71%), Postives = 177/341 (51.91%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYD DVT WSP GR+ Q+EYAMEAVKQGSA +GL+S TH VL  + ++ SEL++HQ
Sbjct: 1   MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKI  VD+HIG++I+GLTAD R+L  +MR EC++  + ++ PLPV RLV  +  K     
Sbjct: 61  KKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSK----- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   Q+ TQR  +RPYGVGLL+ G D+ G H++  CPS NYF+ +A +IG+RSQ+A
Sbjct: 181 -------TQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEK-LTSSICTIAILGVGEPFHILDRET 300
           +TYLER    F + + DEL+K  L A RETL  E+ LT+   +I I+G    F I D + 
Sbjct: 241 RTYLERHMSEFMECNLDELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDD 259

Query: 301 VQKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQ 341
           V   +D  E          ++     Q   AA EPA   D+
Sbjct: 301 VSPFLDGLE----------ERPQRKAQPSQAAEEPAEKADE 259

BLAST of Chy3G064210 vs. ExPASy TrEMBL
Match: A0A0A0LLH5 (Proteasome subunit alpha type OS=Cucumis sativus OX=3659 GN=Csa_2G361820 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 2.4e-145
Identity = 280/353 (79.32%), Postives = 281/353 (79.60%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 281

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 354
           QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI
Sbjct: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of Chy3G064210 vs. ExPASy TrEMBL
Match: A0A5A7VC66 (Proteasome subunit alpha type OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005560 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 7.0e-145
Identity = 279/353 (79.04%), Postives = 280/353 (79.32%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 281

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 354
           QKLIDEFEIVKEDDVPA DQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI
Sbjct: 301 QKLIDEFEIVKEDDVPAADQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of Chy3G064210 vs. ExPASy TrEMBL
Match: A0A1S3BBW8 (Proteasome subunit alpha type OS=Cucumis melo OX=3656 GN=LOC103487987 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 7.0e-145
Identity = 279/353 (79.04%), Postives = 280/353 (79.32%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 281

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 354
           QKLIDEFEIVKEDDVPA DQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI
Sbjct: 301 QKLIDEFEIVKEDDVPAADQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of Chy3G064210 vs. ExPASy TrEMBL
Match: A0A6J1HD20 (Proteasome subunit alpha type OS=Cucurbita moschata OX=3662 GN=LOC111462503 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.2e-141
Identity = 274/354 (77.40%), Postives = 279/354 (78.81%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRS THVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSSRD+LIKDAL+ATRETLQGEKLTSSICTI+ILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSRDDLIKDALMATRETLQGEKLTSSICTISILGVGEPFHILDRETV 282

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEP-AAGTDQGSAPDQGVAPMDI 354
           QKLIDEFEIVKEDDVPA DQD+APEQGDSAAPEP AAGTDQGSAPDQGVAPMDI
Sbjct: 301 QKLIDEFEIVKEDDVPAADQDVAPEQGDSAAPEPAAAGTDQGSAPDQGVAPMDI 282

BLAST of Chy3G064210 vs. ExPASy TrEMBL
Match: A0A6J1KBQ0 (Proteasome subunit alpha type OS=Cucurbita maxima OX=3661 GN=LOC111491854 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.6e-141
Identity = 270/353 (76.49%), Postives = 278/353 (78.75%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRS THVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLY+NCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYFNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSS+D+LIKDAL+ATRETLQGEKLTSS+CTI+ILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSKDDLIKDALMATRETLQGEKLTSSVCTISILGVGEPFHILDRETV 281

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 354
           QKLIDEFEIVKEDDVPA DQD+APE GDSAAPEPAAGTDQGSAPDQGVAPMDI
Sbjct: 301 QKLIDEFEIVKEDDVPAADQDVAPEHGDSAAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of Chy3G064210 vs. NCBI nr
Match: XP_004152689.1 (proteasome subunit alpha type-1-B [Cucumis sativus] >KGN62618.1 hypothetical protein Csa_022520 [Cucumis sativus])

HSP 1 Score: 526 bits (1355), Expect = 1.67e-186
Identity = 280/353 (79.32%), Postives = 281/353 (79.60%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 281

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 353
           QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI
Sbjct: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of Chy3G064210 vs. NCBI nr
Match: XP_008444739.1 (PREDICTED: proteasome subunit alpha type-1-B-like [Cucumis melo] >KAA0065218.1 proteasome subunit alpha type-1-B-like [Cucumis melo var. makuwa])

HSP 1 Score: 525 bits (1351), Expect = 6.80e-186
Identity = 279/353 (79.04%), Postives = 280/353 (79.32%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 281

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 353
           QKLIDEFEIVKEDDVPA DQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI
Sbjct: 301 QKLIDEFEIVKEDDVPAADQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of Chy3G064210 vs. NCBI nr
Match: XP_023546840.1 (proteasome subunit alpha type-1-B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 517 bits (1331), Expect = 7.55e-183
Identity = 273/353 (77.34%), Postives = 279/353 (79.04%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRS THVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSSRD+LIKDAL+ATRETLQGEKLTSSICTI+ILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSRDDLIKDALMATRETLQGEKLTSSICTISILGVGEPFHILDRETV 281

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 353
           Q+LIDEFEIVKEDDVPA DQD+APEQGDSAAPEPAAGTDQGSAPDQGVAPMDI
Sbjct: 301 QQLIDEFEIVKEDDVPAADQDVAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of Chy3G064210 vs. NCBI nr
Match: XP_038886220.1 (proteasome subunit alpha type-1-B-like [Benincasa hispida])

HSP 1 Score: 516 bits (1329), Expect = 1.52e-182
Identity = 274/353 (77.62%), Postives = 277/353 (78.47%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRS THVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 281

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 353
           QKLIDEFEIVK+DD+PA DQDIAPEQGDSA PE AAGTDQGSAPDQGVAPMDI
Sbjct: 301 QKLIDEFEIVKDDDIPAADQDIAPEQGDSAVPEAAAGTDQGSAPDQGVAPMDI 281

BLAST of Chy3G064210 vs. NCBI nr
Match: XP_022961863.1 (proteasome subunit alpha type-1-B-like [Cucurbita moschata])

HSP 1 Score: 514 bits (1323), Expect = 1.30e-181
Identity = 274/354 (77.40%), Postives = 279/354 (78.81%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRS THVVLACVNK+NSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA    
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFENFKDSSRD+LIKDAL+ATRETLQGEKLTSSICTI+ILGVGEPFHILDRETV
Sbjct: 241 KTYLERRFENFKDSSRDDLIKDALMATRETLQGEKLTSSICTISILGVGEPFHILDRETV 282

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAA-GTDQGSAPDQGVAPMDI 353
           QKLIDEFEIVKEDDVPA DQD+APEQGDSAAPEPAA GTDQGSAPDQGVAPMDI
Sbjct: 301 QKLIDEFEIVKEDDVPAADQDVAPEQGDSAAPEPAAAGTDQGSAPDQGVAPMDI 282

BLAST of Chy3G064210 vs. TAIR 10
Match: AT1G47250.1 (20S proteasome alpha subunit F2 )

HSP 1 Score: 409.5 bits (1051), Expect = 2.8e-114
Identity = 220/353 (62.32%), Postives = 250/353 (70.82%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS +HVVLACVNK+ SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           +KIFKVDDHIGVAI+GLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKA    
Sbjct: 61  RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLER+FE+F++SS+++LIKDA++A RETLQGE L SS+CT+++LGV EPFH LD+E++
Sbjct: 241 KTYLERKFESFQESSKEDLIKDAIMAIRETLQGETLKSSLCTVSVLGVDEPFHFLDQESI 277

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 354
           QK+ID FE V E++  A + +  PE    AAP  A   +QG + DQ VAPM+I
Sbjct: 301 QKVIDTFEKVPEEEEDAGEGEAEPE----AAPGAAGTGEQGGSGDQDVAPMEI 277

BLAST of Chy3G064210 vs. TAIR 10
Match: AT5G42790.1 (proteasome alpha subunit F1 )

HSP 1 Score: 403.7 bits (1036), Expect = 1.6e-112
Identity = 221/353 (62.61%), Postives = 243/353 (68.84%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSELSSHQ 60
           MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS +HVVLACVNK+ SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 120
           +KIFKVDDHIGVAI+GLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKA    
Sbjct: 61  RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKA---- 120

Query: 121 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 180
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 181 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240
                   QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA
Sbjct: 181 --------QVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 240

Query: 241 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 300
           KTYLERRFE+F DSSR++LIKDA++A RETLQGE L SS+CT+AILGV EPFH LD+E +
Sbjct: 241 KTYLERRFESFGDSSREDLIKDAILAVRETLQGETLKSSLCTVAILGVDEPFHFLDQEAI 278

Query: 301 QKLIDEFEIVKEDDVPAVDQDIAPEQGDSAAPEPAAGTDQGSAPDQGVAPMDI 354
           QK+ID FE V E++    +      +   AAP    G   G A DQ VAPM++
Sbjct: 301 QKVIDTFEKVPEEEEGEGEAGEGEAEAAEAAPAERGG---GVAGDQDVAPMEM 278

BLAST of Chy3G064210 vs. TAIR 10
Match: AT1G53850.1 (20S proteasome alpha subunit E1 )

HSP 1 Score: 132.9 bits (333), Expect = 5.1e-31
Identity = 98/304 (32.24%), Postives = 139/304 (45.72%), Query Frame = 0

Query: 3   RNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSEL--SSHQ 62
           R +YD  V T+SP GRLFQVEYA+EA+K GS AIG+++   VVLA   +  S L   S  
Sbjct: 5   RTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSV 64

Query: 63  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 122
           +KI ++DDHIG A+SGL AD R L  + R E  N+ ++Y  P+ V      L        
Sbjct: 65  EKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALC------- 124

Query: 123 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 182
                                                           DLA  F  GE  
Sbjct: 125 ------------------------------------------------DLALRFGEGE-- 184

Query: 183 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 242
                       + S  RP+GV LL+ G DE+G  LYY  PSG +++  A AIGS S+ A
Sbjct: 185 ------------EESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGA 234

Query: 243 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 302
            + L+ +F   KD S  E    A+   ++ ++ EK+T +   + I  V   +H+   + V
Sbjct: 245 DSSLQEQFN--KDLSLQEAETIAVSILKQVME-EKVTPN--NVDIAKVAPAYHLYTPQEV 234

Query: 303 QKLI 305
           + +I
Sbjct: 305 EAVI 234

BLAST of Chy3G064210 vs. TAIR 10
Match: AT1G53850.2 (20S proteasome alpha subunit E1 )

HSP 1 Score: 132.9 bits (333), Expect = 5.1e-31
Identity = 98/304 (32.24%), Postives = 139/304 (45.72%), Query Frame = 0

Query: 3   RNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSEL--SSHQ 62
           R +YD  V T+SP GRLFQVEYA+EA+K GS AIG+++   VVLA   +  S L   S  
Sbjct: 5   RTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSV 64

Query: 63  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 122
           +KI ++DDHIG A+SGL AD R L  + R E  N+ ++Y  P+ V      L        
Sbjct: 65  EKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALC------- 124

Query: 123 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 182
                                                           DLA  F  GE  
Sbjct: 125 ------------------------------------------------DLALRFGEGE-- 184

Query: 183 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 242
                       + S  RP+GV LL+ G DE+G  LYY  PSG +++  A AIGS S+ A
Sbjct: 185 ------------EESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGA 234

Query: 243 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 302
            + L+ +F   KD S  E    A+   ++ ++ EK+T +   + I  V   +H+   + V
Sbjct: 245 DSSLQEQFN--KDLSLQEAETIAVSILKQVME-EKVTPN--NVDIAKVAPAYHLYTPQEV 234

Query: 303 QKLI 305
           + +I
Sbjct: 305 EAVI 234

BLAST of Chy3G064210 vs. TAIR 10
Match: AT3G14290.1 (20S proteasome alpha subunit E2 )

HSP 1 Score: 131.7 bits (330), Expect = 1.1e-30
Identity = 97/304 (31.91%), Postives = 139/304 (45.72%), Query Frame = 0

Query: 3   RNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKSNSEL--SSHQ 62
           R +YD  V T+SP GRLFQVEYA+EA+K GS AIG+++   VVLA   +  S L   S  
Sbjct: 5   RTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSV 64

Query: 63  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQSQK 122
           +KI ++DDHIG A+SGL AD R L  + R E  N+ ++Y  P+ V      L        
Sbjct: 65  EKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALC------- 124

Query: 123 ALMANFQRLPLNKLQGSYFHAYEKAVHMGKGNWPAILVYLYPFVSVSIDLATGFDYGEFF 182
                                                           DLA  F  GE  
Sbjct: 125 ------------------------------------------------DLALRFGEGE-- 184

Query: 183 PSTVFLSVQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAA 242
                       + S  RP+GV LL+ G DE+G  LYY  PSG +++  A AIGS S+ A
Sbjct: 185 ------------EESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGA 234

Query: 243 KTYLERRFENFKDSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETV 302
            + L+ +F   KD +  E    A+   ++ ++ EK+T +   + I  V   +H+   + V
Sbjct: 245 DSSLQEQFN--KDITLQEAETIAVSILKQVME-EKVTPN--NVDIAKVAPAYHLYTPQEV 234

Query: 303 QKLI 305
           + +I
Sbjct: 305 EAVI 234

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O237124.0e-11362.32Proteasome subunit alpha type-1-B OS=Arabidopsis thaliana OX=3702 GN=PAF2 PE=1 S... [more]
P340662.2e-11162.61Proteasome subunit alpha type-1-A OS=Arabidopsis thaliana OX=3702 GN=PAF1 PE=1 S... [more]
P524287.1e-9453.91Proteasome subunit alpha type-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PAF1... [more]
Q275626.5e-6342.58Proteasome subunit alpha type-1 OS=Dictyostelium discoideum OX=44689 GN=psmA1 PE... [more]
Q9R1P42.1e-5838.71Proteasome subunit alpha type-1 OS=Mus musculus OX=10090 GN=Psma1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LLH52.4e-14579.32Proteasome subunit alpha type OS=Cucumis sativus OX=3659 GN=Csa_2G361820 PE=3 SV... [more]
A0A5A7VC667.0e-14579.04Proteasome subunit alpha type OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3BBW87.0e-14579.04Proteasome subunit alpha type OS=Cucumis melo OX=3656 GN=LOC103487987 PE=3 SV=1[more]
A0A6J1HD201.2e-14177.40Proteasome subunit alpha type OS=Cucurbita moschata OX=3662 GN=LOC111462503 PE=3... [more]
A0A6J1KBQ01.6e-14176.49Proteasome subunit alpha type OS=Cucurbita maxima OX=3661 GN=LOC111491854 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_004152689.11.67e-18679.32proteasome subunit alpha type-1-B [Cucumis sativus] >KGN62618.1 hypothetical pro... [more]
XP_008444739.16.80e-18679.04PREDICTED: proteasome subunit alpha type-1-B-like [Cucumis melo] >KAA0065218.1 p... [more]
XP_023546840.17.55e-18377.34proteasome subunit alpha type-1-B-like [Cucurbita pepo subsp. pepo][more]
XP_038886220.11.52e-18277.62proteasome subunit alpha type-1-B-like [Benincasa hispida][more]
XP_022961863.11.30e-18177.40proteasome subunit alpha type-1-B-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT1G47250.12.8e-11462.3220S proteasome alpha subunit F2 [more]
AT5G42790.11.6e-11262.61proteasome alpha subunit F1 [more]
AT1G53850.15.1e-3132.2420S proteasome alpha subunit E1 [more]
AT1G53850.25.1e-3132.2420S proteasome alpha subunit E1 [more]
AT3G14290.11.1e-3031.9120S proteasome alpha subunit E2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000426Proteasome alpha-subunit, N-terminal domainSMARTSM00948Proteasome_A_N_2coord: 6..28
e-value: 4.5E-10
score: 49.5
IPR000426Proteasome alpha-subunit, N-terminal domainPFAMPF10584Proteasome_A_Ncoord: 6..28
e-value: 9.1E-14
score: 50.8
IPR000426Proteasome alpha-subunit, N-terminal domainPROSITEPS00388PROTEASOME_ALPHA_1coord: 6..28
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 29..119
e-value: 2.3E-25
score: 89.2
coord: 184..285
e-value: 3.0E-25
score: 88.8
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 1..149
e-value: 2.2E-46
score: 160.0
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 182..319
e-value: 9.1E-31
score: 108.9
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 188..307
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 4..118
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 315..353
NoneNo IPR availablePANTHERPTHR11599:SF182PROTEASOME SUBUNIT ALPHA TYPEcoord: 188..344
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 188..344
coord: 1..118
NoneNo IPR availablePANTHERPTHR11599:SF182PROTEASOME SUBUNIT ALPHA TYPEcoord: 1..118
IPR023332Proteasome alpha-type subunitPROSITEPS51475PROTEASOME_ALPHA_2coord: 21..304
score: 67.588173
IPR035144Proteasome subunit alpha 1CDDcd03749proteasome_alpha_type_1coord: 6..287
e-value: 8.94987E-133
score: 375.475

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G064210.1Chy3G064210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010498 proteasomal protein catabolic process
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019773 proteasome core complex, alpha-subunit complex
cellular_component GO:0005839 proteasome core complex