Chy3G061460 (gene) Cucumber (hystrix) v1

Overview
NameChy3G061460
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptiongibberellin 3-beta-dioxygenase 2-like
LocationchrH03: 14863951 .. 14865657 (-)
RNA-Seq ExpressionChy3G061460
SyntenyChy3G061460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCACTCTCTCTCAAACTTATCTAGATCACCCTCTTATTCACCATCATCACATCGTTCCTTTAGACTTTGATTCATTACGAACGATTCCCGATTCTCACGATTGGTTCAATTCCTCCCTCGAAACCCCTTCTTCTTCCTCTTGTCATCACCATACTAATGTAGATGTTTCGATCCCTTTGATTGATTTAACTGACCTTGATGCAATTTCGCTCATCAATAATGCATGCGAGACGTGGGGAGTGTTCCAATTGATCAATCATAACGTGCCAATGAGCCTAATTGAGAGAGCTGAAGGCGAAACGAGACGACTCTTCGATCTTCCTATGACACGAAAGCTGAAGGCACTTCGAGCTCCAGGAGACGTCACTGGATATGGATTGCCTCGAATAACGCCATTTTTCTCTAAGTATATGTGGCACGAAGGGTTTACTATTATGGGGCCTTCCATTGGTCATGCCTCACAACTTTGGCCTTCTAACTACCAACCGTTTTGGTAACTCCTCCATGCACTATTACACACGTAATTAACTAATTTTCTATTTTATTAGTTGTGGAGTACTTGGATTTATATGTCCTAATTAATAATGGTGAGATCTATTTTCAATATATTATAAATAAACTAATTAAACAAAACCGAGTAGTGATCATATAAGAAAATTGTTACAAAAAAAGAAAAGTTCAATTTATTAAGAATGCTCTAAGTTTGTATTTTATGGAATATTTAAACCATTATTATATATTATATATGCTCTTTTAAGTTATTATTTGTGGAATAATTTTGGATTATTTTACTATTATATTTTTATTAAAACTATTTAAAAGATATAAAAATAGTAATAAAGGAAGATTCTAATGTTTCAATTTTAAATGATAGTAATAATGGAACAACGTAAAATTAAATTTAGATACCGTACCTAATTAAATATAGGTTTGAGATTTTAGGAACATACAGGTGATAATATAACCTTTAGTTTATAATTCTTTTTGGTCCTTCAAATGAATATATTAAGGCATAATAACTTTGGAAAAAAAGGTTGTTTTTATTTATATATTAATTATTAAACATATAATCATATAAGAAATCAGATATTTTAATTTTATAATGAAATAGTTAATAATGAATTTTGAAATGTAGCGATGTAATGGAAGAGTATCAAAGGAAAATGAAGTCCTTAGCGGAGCAAATCACACATTCAATCTTCAATTACCTTAAAATCTCTGATGCTGCAAACTGGCTCCATACAGCTGGATCAGCCGGAGCCGCCGCCTGCAGCACCGCCCTTCAACTTAACTGCTACCCTCGCTGCCCGGACCCGACTCGGGTCATGGGCTTAGCCCCACACACCGACACATTTCTATTAACCATCCTCCATCAGACCCGTACGTGCGGCCTCCAAGTTTTCAGGGATGGGTCCGGCTGGGTACCGGTGGCACCCGTACCTGGTGCACTGGTCCTCAACGTCGGAGACTTGTTCCACATCCTTTCGAATGGGCGGTTCCCAAATGTTCTACATCGGGTCGTCGTTGACCCGACCCGACGCCGGCTATCTATGGCTTATTTCTATGGGCCGCCACCGGATTTCTGTGTATCGCCGTTGTATGATCCACCGGAATCTCCATGTTACCGGTCTGTGATGGTGAAAGATTATGTTCGGTTGAAGGCGAAGAATCTCGAAAATGCCCTTTCAATGATCCGATTATGA

mRNA sequence

ATGACCACTCTCTCTCAAACTTATCTAGATCACCCTCTTATTCACCATCATCACATCGTTCCTTTAGACTTTGATTCATTACGAACGATTCCCGATTCTCACGATTGGTTCAATTCCTCCCTCGAAACCCCTTCTTCTTCCTCTTGTCATCACCATACTAATGTAGATGTTTCGATCCCTTTGATTGATTTAACTGACCTTGATGCAATTTCGCTCATCAATAATGCATGCGAGACGTGGGGAGTGTTCCAATTGATCAATCATAACGTGCCAATGAGCCTAATTGAGAGAGCTGAAGGCGAAACGAGACGACTCTTCGATCTTCCTATGACACGAAAGCTGAAGGCACTTCGAGCTCCAGGAGACGTCACTGGATATGGATTGCCTCGAATAACGCCATTTTTCTCTAAGTATATGTGGCACGAAGGGTTTACTATTATGGGGCCTTCCATTGGTCATGCCTCACAACTTTGGCCTTCTAACTACCAACCGTTTTGCGATGTAATGGAAGAGTATCAAAGGAAAATGAAGTCCTTAGCGGAGCAAATCACACATTCAATCTTCAATTACCTTAAAATCTCTGATGCTGCAAACTGGCTCCATACAGCTGGATCAGCCGGAGCCGCCGCCTGCAGCACCGCCCTTCAACTTAACTGCTACCCTCGCTGCCCGGACCCGACTCGGGTCATGGGCTTAGCCCCACACACCGACACATTTCTATTAACCATCCTCCATCAGACCCGTACGTGCGGCCTCCAAGTTTTCAGGGATGGGTCCGGCTGGGTACCGGTGGCACCCGTACCTGGTGCACTGGTCCTCAACGTCGGAGACTTGTTCCACATCCTTTCGAATGGGCGGTTCCCAAATGTTCTACATCGGGTCGTCGTTGACCCGACCCGACGCCGGCTATCTATGGCTTATTTCTATGGGCCGCCACCGGATTTCTGTGTATCGCCGTTGTATGATCCACCGGAATCTCCATGTTACCGGTCTGTGATGGTGAAAGATTATGTTCGGTTGAAGGCGAAGAATCTCGAAAATGCCCTTTCAATGATCCGATTATGA

Coding sequence (CDS)

ATGACCACTCTCTCTCAAACTTATCTAGATCACCCTCTTATTCACCATCATCACATCGTTCCTTTAGACTTTGATTCATTACGAACGATTCCCGATTCTCACGATTGGTTCAATTCCTCCCTCGAAACCCCTTCTTCTTCCTCTTGTCATCACCATACTAATGTAGATGTTTCGATCCCTTTGATTGATTTAACTGACCTTGATGCAATTTCGCTCATCAATAATGCATGCGAGACGTGGGGAGTGTTCCAATTGATCAATCATAACGTGCCAATGAGCCTAATTGAGAGAGCTGAAGGCGAAACGAGACGACTCTTCGATCTTCCTATGACACGAAAGCTGAAGGCACTTCGAGCTCCAGGAGACGTCACTGGATATGGATTGCCTCGAATAACGCCATTTTTCTCTAAGTATATGTGGCACGAAGGGTTTACTATTATGGGGCCTTCCATTGGTCATGCCTCACAACTTTGGCCTTCTAACTACCAACCGTTTTGCGATGTAATGGAAGAGTATCAAAGGAAAATGAAGTCCTTAGCGGAGCAAATCACACATTCAATCTTCAATTACCTTAAAATCTCTGATGCTGCAAACTGGCTCCATACAGCTGGATCAGCCGGAGCCGCCGCCTGCAGCACCGCCCTTCAACTTAACTGCTACCCTCGCTGCCCGGACCCGACTCGGGTCATGGGCTTAGCCCCACACACCGACACATTTCTATTAACCATCCTCCATCAGACCCGTACGTGCGGCCTCCAAGTTTTCAGGGATGGGTCCGGCTGGGTACCGGTGGCACCCGTACCTGGTGCACTGGTCCTCAACGTCGGAGACTTGTTCCACATCCTTTCGAATGGGCGGTTCCCAAATGTTCTACATCGGGTCGTCGTTGACCCGACCCGACGCCGGCTATCTATGGCTTATTTCTATGGGCCGCCACCGGATTTCTGTGTATCGCCGTTGTATGATCCACCGGAATCTCCATGTTACCGGTCTGTGATGGTGAAAGATTATGTTCGGTTGAAGGCGAAGAATCTCGAAAATGCCCTTTCAATGATCCGATTATGA

Protein sequence

MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIPLIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL*
Homology
BLAST of Chy3G061460 vs. ExPASy Swiss-Prot
Match: Q39103 (Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 SV=2)

HSP 1 Score: 320.5 bits (820), Expect = 2.4e-86
Identity = 171/361 (47.37%), Postives = 223/361 (61.77%), Query Frame = 0

Query: 4   LSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDW-------FNSSLETPSSSSCHHHTNVD 63
           L+  +  HP+   H  +P DF SLR +PDS+ W       F+++   P++          
Sbjct: 5   LTDVFRGHPIHLPHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGE-------- 64

Query: 64  VSIPLIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKA 123
            +IPLIDL   DA + I +AC TWG FQ+ NH VP+ L++  E  T  LF LP+ RKLK+
Sbjct: 65  -NIPLIDLDHPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKS 124

Query: 124 LRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKM 183
            R+   V+GYG+ RI  FF+K MW EGFTI G  +    +LWP ++  +CD++EEY+  M
Sbjct: 125 ARSETGVSGYGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHM 184

Query: 184 KSLAEQITHSIFNYLKISDA-ANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPH 243
           K LA ++     N L +S+    W   +     A    ALQLN YP CP+P R MGLA H
Sbjct: 185 KKLASKLMWLALNSLGVSEEDIEWASLSSDLNWA--QAALQLNHYPVCPEPDRAMGLAAH 244

Query: 244 TDTFLLTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVV 303
           TD+ LLTIL+Q  T GLQVFRD  GWV V P PG+LV+NVGDLFHILSNG F +VLHR  
Sbjct: 245 TDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRAR 304

Query: 304 VDPTRRRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMI 354
           V+ TR RLS+A+ +GP  D  +SP   L  P ESP Y+SV  K+Y+R KA +   ALSMI
Sbjct: 305 VNQTRARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMI 353

BLAST of Chy3G061460 vs. ExPASy Swiss-Prot
Match: O24648 (Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 5.4e-86
Identity = 170/360 (47.22%), Postives = 233/360 (64.72%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           M +LS+ Y  HP +H +H  P DF+SL+ +P+S++W +    T   S+ +       ++P
Sbjct: 1   MPSLSEAYRAHP-VHVNHKHP-DFNSLQELPESYNWTHLDDHTLIDSN-NIMKESTTTVP 60

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           +IDL D +A  LI  AC+TWGV+Q++NH +P+SL+E  +   + LF LP  +K KA R+P
Sbjct: 61  VIDLNDPNASKLIGLACKTWGVYQVMNHGIPLSLLEDIQWLGQTLFSLPSHQKHKATRSP 120

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180
             V+GYG+ RI+ FF K MW+EGFTI+G  + H  +LWP +Y  FCD++ +Y   MK LA
Sbjct: 121 DGVSGYGIARISSFFPKLMWYEGFTIVGSPLDHFRELWPQDYTRFCDIVVQYDETMKKLA 180

Query: 181 EQITHSIFNYLKIS-DAANWLHTAGSAG--AAACSTALQLNCYPRCPDPTRVMGLAPHTD 240
             +   + + L I+ +   W   AGS      AC+ ALQLN YP CPDP   MGLAPHTD
Sbjct: 181 GTLMCLMLDSLGITKEDIKW---AGSKAQFEKACA-ALQLNSYPSCPDPDHAMGLAPHTD 240

Query: 241 TFLLTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVD 300
           +  LTIL Q    GLQV R+GSGW+ V P+ G LV+NVGDLFHILSNG +P+VLHRV+V+
Sbjct: 241 STFLTILSQNDISGLQVNREGSGWITVPPLQGGLVVNVGDLFHILSNGLYPSVLHRVLVN 300

Query: 301 PTRRRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 355
            TR+R S+AY YGPP +  + P   L  P + P YRSV   +Y+  KAK+   ALS +RL
Sbjct: 301 RTRQRFSVAYLYGPPSNVEICPHAKLIGPTKPPLYRSVTWNEYLGTKAKHFNKALSSVRL 353

BLAST of Chy3G061460 vs. ExPASy Swiss-Prot
Match: Q9ZT84 (Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 SV=2)

HSP 1 Score: 307.8 bits (787), Expect = 1.6e-82
Identity = 161/356 (45.22%), Postives = 217/356 (60.96%), Query Frame = 0

Query: 2   TTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIPL 61
           +TLS  +  HP IH     P DF SL   PDS+ W      TP        +  D ++PL
Sbjct: 3   STLSDVFRSHP-IHIPLSNPPDFKSL---PDSYTW------TPKDDLLFSASASDETLPL 62

Query: 62  IDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAPG 121
           IDL+D+   +L+ +AC TWG FQ+ NH VP  L++  E  T  LF LP+ RKLKA R+  
Sbjct: 63  IDLSDIHVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSEN 122

Query: 122 DVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLAE 181
            V+GYG+ RI  FF+K MW EGFT++G  +    +LWPS++  +C+++EEY+  M+ LA 
Sbjct: 123 GVSGYGVARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAA 182

Query: 182 QITHSIFNYLKISDA-ANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 241
           ++       L + +    W     ++        +QLN YP+CP+P R MGLA HTD+ L
Sbjct: 183 KLMWFALGSLGVEEKDIQW--AGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTL 242

Query: 242 LTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 301
           +TIL+Q  T GLQVFRD  GWV   PVPG+LV+NVGDL HIL+NG FP+VLHR  V+  R
Sbjct: 243 MTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVR 302

Query: 302 RRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIR 354
            R SMAY +GPP D  +SP   L DP +SP Y S+  K Y+  KA +   +LS+IR
Sbjct: 303 SRFSMAYLWGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of Chy3G061460 vs. ExPASy Swiss-Prot
Match: Q9C971 (Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 SV=1)

HSP 1 Score: 291.6 bits (745), Expect = 1.2e-77
Identity = 150/338 (44.38%), Postives = 216/338 (63.91%), Query Frame = 0

Query: 26  SLRTIPDSHDW---FNSSLETPSSSSCHHHTNVDVSIPLIDLTDLDAISLINNACETWGV 85
           SL+T+P+S  W      SL  PSS+       V+ SIP+IDL++ D  +LI +A +TWG 
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAV--SFDAVEESIPVIDLSNPDVTTLIGDASKTWGA 74

Query: 86  FQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHE 145
           FQ+ NH +   L++  E  ++ LFD+P  RKL+A  +   V+GYG PRI+PFF K MW E
Sbjct: 75  FQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSE 134

Query: 146 GFTIMGPSI-GHASQLWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKIS-DAANWL 205
           GFTI   S   H + LWP ++  +C +++EY  +M+ LA ++ + I   L ++ +   W 
Sbjct: 135 GFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWA 194

Query: 206 HTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGSG 265
           H    +G+     A++LN YP CP+P R MGLA HTD+ +LTILHQ+ T GLQVFR+ SG
Sbjct: 195 HKLEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESG 254

Query: 266 WVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFY-GPPPDFCVSP 325
           WV V P PG LV+N+GDLFHILSNG+ P+V+HR  V+ TR R+S+AY + GP  D  ++P
Sbjct: 255 WVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAP 314

Query: 326 ---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 355
              L  P E   YRS+  K+Y+++K +  + A+  IR+
Sbjct: 315 ISKLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of Chy3G061460 vs. ExPASy Swiss-Prot
Match: Q9SVS8 (Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 8.7e-76
Identity = 147/360 (40.83%), Postives = 222/360 (61.67%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           M++++Q + ++P ++   I+PLDF + +T+PDSH W     ET S             IP
Sbjct: 1   MSSVTQLFKNNP-VNRDRIIPLDFTNTKTLPDSHVWSKPEPETTSG-----------PIP 60

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           +I L++ +   L+  ACE WGVF + +H V  SL+   + + +RLF LPM RK+ A+R+P
Sbjct: 61  VISLSNPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSP 120

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSI-GHASQLWPSNYQPFCDVMEEYQRKMKSL 180
            + TGYG+ RI+ F+ K MW EGF++MG S+  HA+ LWP ++  FC+VMEEYQ+ M  L
Sbjct: 121 DESTGYGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDL 180

Query: 181 AEQITHSIFNYLKIS-DAANWL-HTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTD 240
           + ++   +   L ++ +   WL      +G  +  + LQLN YP CPDP   MGLAPHTD
Sbjct: 181 SHRLISMLMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTD 240

Query: 241 TFLLTILHQTRTCGLQV---FRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRV 300
           + LLTIL+Q    GL++     +GS W+ V P+ G+LV+ +GDL HI+SNG+F + +HR 
Sbjct: 241 SSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRA 300

Query: 301 VVDPTRRRLSMAYFYGPPPDFCVSPL-YDPPESPCYRSVMVKDYVRLKAKNLENALSMIR 354
           VV+ T  R+S AYF GPP +  + PL  D    P YR ++ ++Y+  KA +   AL++ R
Sbjct: 301 VVNKTHHRVSAAYFAGPPKNLQIGPLTSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

BLAST of Chy3G061460 vs. ExPASy TrEMBL
Match: S0BE23 (GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox2 PE=2 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 3.7e-202
Identity = 341/354 (96.33%), Postives = 346/354 (97.74%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS HHHTNV+VSIP
Sbjct: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS-HHHTNVNVSIP 60

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           LIDLTD +AISLI NACETWGVFQLINH+VP+SLIERAEGETRRLFDLPMTRKLKALRAP
Sbjct: 61  LIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRAP 120

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180
           GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA
Sbjct: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180

Query: 181 EQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240
           EQIT SIFNYLKISD ANWLH+AGS  AAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL
Sbjct: 181 EQITRSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240

Query: 241 LTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 300
           LTILHQTRTCGLQVFRDG GWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR
Sbjct: 241 LTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 300

Query: 301 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 355
           RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL
Sbjct: 301 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 353

BLAST of Chy3G061460 vs. ExPASy TrEMBL
Match: A0A0A0LPF2 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379300 PE=3 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 3.7e-202
Identity = 341/354 (96.33%), Postives = 346/354 (97.74%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS HHHTNV+VSIP
Sbjct: 20  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS-HHHTNVNVSIP 79

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           LIDLTD +AISLI NACETWGVFQLINH+VP+SLIERAEGETRRLFDLPMTRKLKALRAP
Sbjct: 80  LIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRAP 139

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180
           GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA
Sbjct: 140 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 199

Query: 181 EQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240
           EQIT SIFNYLKISD ANWLH+AGS  AAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL
Sbjct: 200 EQITRSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 259

Query: 241 LTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 300
           LTILHQTRTCGLQVFRDG GWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR
Sbjct: 260 LTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 319

Query: 301 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 355
           RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL
Sbjct: 320 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 372

BLAST of Chy3G061460 vs. ExPASy TrEMBL
Match: A0A5A7VCQ3 (Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G002910 PE=3 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.9e-190
Identity = 321/354 (90.68%), Postives = 335/354 (94.63%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           MTTLSQTY DHPLIHHHHIVPLDFDSLRTIPDSHDW +SS E PSSSS  +HTNVDVSIP
Sbjct: 1   MTTLSQTYRDHPLIHHHHIVPLDFDSLRTIPDSHDWLHSSPEIPSSSSSCYHTNVDVSIP 60

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           LIDLTD DAI+LI NACETWGVFQLINH+VPMSLIE+AEGETRRLFDLPMTRKLKALR P
Sbjct: 61  LIDLTDHDAIALIGNACETWGVFQLINHDVPMSLIEKAEGETRRLFDLPMTRKLKALRTP 120

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180
            DVTGYGLPRITPFFSKYMWHEGFTIMGP I HASQLWPSNYQPFCDVMEEYQRKMK+LA
Sbjct: 121 RDVTGYGLPRITPFFSKYMWHEGFTIMGPPIDHASQLWPSNYQPFCDVMEEYQRKMKALA 180

Query: 181 EQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240
           EQIT+ IFNYLKISD ANWLH+ G+  AAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL
Sbjct: 181 EQITNLIFNYLKISDGANWLHSTGA--AAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240

Query: 241 LTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 300
           LTILHQTRTCGLQ+FRDGSGWVPVAP+PGALVLNVGDLFHILSNGRFPNVLHRVVVDPT+
Sbjct: 241 LTILHQTRTCGLQIFRDGSGWVPVAPLPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTQ 300

Query: 301 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 355
           RRLSMAYFYGPPPDFCVSPL DPPE+PCYR V+VKDYVRLKAKNLENALSMIRL
Sbjct: 301 RRLSMAYFYGPPPDFCVSPLNDPPETPCYRPVVVKDYVRLKAKNLENALSMIRL 352

BLAST of Chy3G061460 vs. ExPASy TrEMBL
Match: A0A1S3BCU4 (gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488262 PE=3 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 7.2e-190
Identity = 322/356 (90.45%), Postives = 336/356 (94.38%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCH--HHTNVDVS 60
           MTTLSQTY DHPLIHHHHIVPLDFDSLRTIPDSHDW +SS ETPSSSS    +HTNVDVS
Sbjct: 16  MTTLSQTYRDHPLIHHHHIVPLDFDSLRTIPDSHDWLHSSPETPSSSSSSSCYHTNVDVS 75

Query: 61  IPLIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALR 120
           IPLIDLTD DAI+LI NACETWGVFQLINH+VPMSLIE+AEGETRRLFDLPMTRKLKALR
Sbjct: 76  IPLIDLTDHDAIALIGNACETWGVFQLINHDVPMSLIEKAEGETRRLFDLPMTRKLKALR 135

Query: 121 APGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKS 180
            P DVTGYGLPRITPFFSKYMWHEGFTIMGP I HASQLWPSNYQPFCDVMEEYQRKMK+
Sbjct: 136 TPRDVTGYGLPRITPFFSKYMWHEGFTIMGPPIDHASQLWPSNYQPFCDVMEEYQRKMKA 195

Query: 181 LAEQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDT 240
           LAEQIT+ IFNYLKISD ANWLH+ G+  AAACSTALQLNCYPRCPDPTRVMGLAPHTDT
Sbjct: 196 LAEQITNLIFNYLKISDCANWLHSTGA--AAACSTALQLNCYPRCPDPTRVMGLAPHTDT 255

Query: 241 FLLTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDP 300
           FLLTILHQTRTCGLQ+FRDGSGWVPVAP+PGALVLNVGDLFHILSNGRFPNVLHRVVVDP
Sbjct: 256 FLLTILHQTRTCGLQIFRDGSGWVPVAPLPGALVLNVGDLFHILSNGRFPNVLHRVVVDP 315

Query: 301 TRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 355
           T+RRLSMAYFYGPPPDFCVSPL DPPE+PCYR V+VKDYVRLKAKNLENALSMIRL
Sbjct: 316 TQRRLSMAYFYGPPPDFCVSPLNDPPETPCYRPVVVKDYVRLKAKNLENALSMIRL 369

BLAST of Chy3G061460 vs. ExPASy TrEMBL
Match: I0J1I8 (Gibberellin 3-oxidase OS=Cucurbita maxima OX=3661 GN=ga3ox4 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.5e-163
Identity = 283/357 (79.27%), Postives = 306/357 (85.71%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           MTTLSQ Y DHPLIH HHIVPLDFDSLRT+PDSHDW +SS +T        HTN DVSIP
Sbjct: 1   MTTLSQAYRDHPLIHLHHIVPLDFDSLRTVPDSHDWPHSSYDT-----LKLHTNGDVSIP 60

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           LID++D +   LI N C+TWGVFQL NH VP SL+ERAE E  RLFDLPMTRKLKALRAP
Sbjct: 61  LIDMSDPNVAMLIGNVCKTWGVFQLTNHGVPTSLVERAEAEAGRLFDLPMTRKLKALRAP 120

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180
           GD TGYGLPRITPFFSKYMWHEGFTIMG S  HASQLW SNYQPFCDVMEEYQ+ MKSLA
Sbjct: 121 GDATGYGLPRITPFFSKYMWHEGFTIMGSSAEHASQLWSSNYQPFCDVMEEYQKNMKSLA 180

Query: 181 EQITHSIFNYLKISDAA---NWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTD 240
           E+ITH I N+LKI D A   NWLH+AG+  AAACSTALQLNCYPRCPDPTRVMGLAPHTD
Sbjct: 181 EKITHLILNFLKIPDDAEISNWLHSAGA--AAACSTALQLNCYPRCPDPTRVMGLAPHTD 240

Query: 241 TFLLTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVD 300
           TFLLTILHQ+ TCGLQ+FRDG GW+PV PV GALVLNVGDLFHILSNGRFPNVLHRVVVD
Sbjct: 241 TFLLTILHQSSTCGLQIFRDGFGWIPVTPVAGALVLNVGDLFHILSNGRFPNVLHRVVVD 300

Query: 301 PTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 355
           PTRRRLSMAYF+GPPPDFCV+P  +PPESP +RSV+VK+YV LKAKNLE ALSMI +
Sbjct: 301 PTRRRLSMAYFFGPPPDFCVTPFNNPPESPRFRSVVVKEYVGLKAKNLECALSMIEI 350

BLAST of Chy3G061460 vs. NCBI nr
Match: NP_001274405.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >CCD28483.1 GA 3-oxidase [Cucumis sativus])

HSP 1 Score: 711 bits (1834), Expect = 2.58e-258
Identity = 341/354 (96.33%), Postives = 346/354 (97.74%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS HHHTNV+VSIP
Sbjct: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS-HHHTNVNVSIP 60

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           LIDLTD +AISLI NACETWGVFQLINH+VP+SLIERAEGETRRLFDLPMTRKLKALRAP
Sbjct: 61  LIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRAP 120

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180
           GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA
Sbjct: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180

Query: 181 EQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240
           EQIT SIFNYLKISD ANWLH+AGS  AAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL
Sbjct: 181 EQITRSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240

Query: 241 LTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 300
           LTILHQTRTCGLQVFRDG GWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR
Sbjct: 241 LTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 300

Query: 301 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 354
           RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL
Sbjct: 301 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 353

BLAST of Chy3G061460 vs. NCBI nr
Match: KGN62899.1 (hypothetical protein Csa_021969 [Cucumis sativus])

HSP 1 Score: 711 bits (1834), Expect = 5.29e-258
Identity = 341/354 (96.33%), Postives = 346/354 (97.74%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS HHHTNV+VSIP
Sbjct: 20  MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSS-HHHTNVNVSIP 79

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           LIDLTD +AISLI NACETWGVFQLINH+VP+SLIERAEGETRRLFDLPMTRKLKALRAP
Sbjct: 80  LIDLTDPNAISLIGNACETWGVFQLINHDVPVSLIERAEGETRRLFDLPMTRKLKALRAP 139

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180
           GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA
Sbjct: 140 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 199

Query: 181 EQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240
           EQIT SIFNYLKISD ANWLH+AGS  AAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL
Sbjct: 200 EQITRSIFNYLKISDGANWLHSAGSTEAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 259

Query: 241 LTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 300
           LTILHQTRTCGLQVFRDG GWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR
Sbjct: 260 LTILHQTRTCGLQVFRDGFGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 319

Query: 301 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 354
           RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL
Sbjct: 320 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 372

BLAST of Chy3G061460 vs. NCBI nr
Match: KAA0064970.1 (gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 672 bits (1733), Expect = 6.19e-243
Identity = 321/354 (90.68%), Postives = 335/354 (94.63%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           MTTLSQTY DHPLIHHHHIVPLDFDSLRTIPDSHDW +SS E PSSSS  +HTNVDVSIP
Sbjct: 1   MTTLSQTYRDHPLIHHHHIVPLDFDSLRTIPDSHDWLHSSPEIPSSSSSCYHTNVDVSIP 60

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           LIDLTD DAI+LI NACETWGVFQLINH+VPMSLIE+AEGETRRLFDLPMTRKLKALR P
Sbjct: 61  LIDLTDHDAIALIGNACETWGVFQLINHDVPMSLIEKAEGETRRLFDLPMTRKLKALRTP 120

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180
            DVTGYGLPRITPFFSKYMWHEGFTIMGP I HASQLWPSNYQPFCDVMEEYQRKMK+LA
Sbjct: 121 RDVTGYGLPRITPFFSKYMWHEGFTIMGPPIDHASQLWPSNYQPFCDVMEEYQRKMKALA 180

Query: 181 EQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240
           EQIT+ IFNYLKISD ANWLH+ G+A  AACSTALQLNCYPRCPDPTRVMGLAPHTDTFL
Sbjct: 181 EQITNLIFNYLKISDGANWLHSTGAA--AACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 240

Query: 241 LTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 300
           LTILHQTRTCGLQ+FRDGSGWVPVAP+PGALVLNVGDLFHILSNGRFPNVLHRVVVDPT+
Sbjct: 241 LTILHQTRTCGLQIFRDGSGWVPVAPLPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTQ 300

Query: 301 RRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 354
           RRLSMAYFYGPPPDFCVSPL DPPE+PCYR V+VKDYVRLKAKNLENALSMIRL
Sbjct: 301 RRLSMAYFYGPPPDFCVSPLNDPPETPCYRPVVVKDYVRLKAKNLENALSMIRL 352

BLAST of Chy3G061460 vs. NCBI nr
Match: XP_008445137.1 (PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo])

HSP 1 Score: 670 bits (1728), Expect = 6.76e-242
Identity = 322/356 (90.45%), Postives = 336/356 (94.38%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCH--HHTNVDVS 60
           MTTLSQTY DHPLIHHHHIVPLDFDSLRTIPDSHDW +SS ETPSSSS    +HTNVDVS
Sbjct: 16  MTTLSQTYRDHPLIHHHHIVPLDFDSLRTIPDSHDWLHSSPETPSSSSSSSCYHTNVDVS 75

Query: 61  IPLIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALR 120
           IPLIDLTD DAI+LI NACETWGVFQLINH+VPMSLIE+AEGETRRLFDLPMTRKLKALR
Sbjct: 76  IPLIDLTDHDAIALIGNACETWGVFQLINHDVPMSLIEKAEGETRRLFDLPMTRKLKALR 135

Query: 121 APGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKS 180
            P DVTGYGLPRITPFFSKYMWHEGFTIMGP I HASQLWPSNYQPFCDVMEEYQRKMK+
Sbjct: 136 TPRDVTGYGLPRITPFFSKYMWHEGFTIMGPPIDHASQLWPSNYQPFCDVMEEYQRKMKA 195

Query: 181 LAEQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDT 240
           LAEQIT+ IFNYLKISD ANWLH+ G+A  AACSTALQLNCYPRCPDPTRVMGLAPHTDT
Sbjct: 196 LAEQITNLIFNYLKISDCANWLHSTGAA--AACSTALQLNCYPRCPDPTRVMGLAPHTDT 255

Query: 241 FLLTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDP 300
           FLLTILHQTRTCGLQ+FRDGSGWVPVAP+PGALVLNVGDLFHILSNGRFPNVLHRVVVDP
Sbjct: 256 FLLTILHQTRTCGLQIFRDGSGWVPVAPLPGALVLNVGDLFHILSNGRFPNVLHRVVVDP 315

Query: 301 TRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 354
           T+RRLSMAYFYGPPPDFCVSPL DPPE+PCYR V+VKDYVRLKAKNLENALSMIRL
Sbjct: 316 TQRRLSMAYFYGPPPDFCVSPLNDPPETPCYRPVVVKDYVRLKAKNLENALSMIRL 369

BLAST of Chy3G061460 vs. NCBI nr
Match: XP_038886716.1 (gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida])

HSP 1 Score: 618 bits (1593), Expect = 1.33e-221
Identity = 302/357 (84.59%), Postives = 317/357 (88.80%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           MTTLSQTY DHPLIHHHHIVPLD DSLRTIPDSHDW  SS +TPSSS   HHTN DVSIP
Sbjct: 1   MTTLSQTYRDHPLIHHHHIVPLDLDSLRTIPDSHDWSGSSPDTPSSSG--HHTNEDVSIP 60

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
            IDL D +A  LI NACETWGVFQLINH+VP++ I+ AEGETRRLFDLPMTRKLKALRAP
Sbjct: 61  SIDLNDPNATMLIGNACETWGVFQLINHDVPLTFIDTAEGETRRLFDLPMTRKLKALRAP 120

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLA 180
              TGYGLPRITPFFSKYMWHEGFTIMG S+ HASQLWPSNYQPFCDVMEEYQRKMKSLA
Sbjct: 121 DHATGYGLPRITPFFSKYMWHEGFTIMGSSVDHASQLWPSNYQPFCDVMEEYQRKMKSLA 180

Query: 181 EQITHSIFNYLKISDAA---NWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTD 240
           EQITH IFN+LKIS+ A   NWLH   S  AA CSTALQLNCYPRCPDPTRVMGLAPHTD
Sbjct: 181 EQITHFIFNFLKISNDAKITNWLH---STEAATCSTALQLNCYPRCPDPTRVMGLAPHTD 240

Query: 241 TFLLTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVD 300
           TFLLTILHQ+RTCGLQVFRDG GWV VAPV GALV+NVGDLFHI+SNGRFPNVLHRVVVD
Sbjct: 241 TFLLTILHQSRTCGLQVFRDGFGWVSVAPVAGALVINVGDLFHIMSNGRFPNVLHRVVVD 300

Query: 301 PTRRRLSMAYFYGPPPDFCVSPLYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 354
           PTRRRLSMAYFYGPPPDFCVSPL DP +SP YRSV+VKDYVRLK KNLENALSMIR+
Sbjct: 301 PTRRRLSMAYFYGPPPDFCVSPLNDPLKSPRYRSVVVKDYVRLKGKNLENALSMIRI 352

BLAST of Chy3G061460 vs. TAIR 10
Match: AT1G15550.1 (gibberellin 3-oxidase 1 )

HSP 1 Score: 320.5 bits (820), Expect = 1.7e-87
Identity = 171/361 (47.37%), Postives = 223/361 (61.77%), Query Frame = 0

Query: 4   LSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDW-------FNSSLETPSSSSCHHHTNVD 63
           L+  +  HP+   H  +P DF SLR +PDS+ W       F+++   P++          
Sbjct: 5   LTDVFRGHPIHLPHSHIP-DFTSLRELPDSYKWTPKDDLLFSAAPSPPATGE-------- 64

Query: 64  VSIPLIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKA 123
            +IPLIDL   DA + I +AC TWG FQ+ NH VP+ L++  E  T  LF LP+ RKLK+
Sbjct: 65  -NIPLIDLDHPDATNQIGHACRTWGAFQISNHGVPLGLLQDIEFLTGSLFGLPVQRKLKS 124

Query: 124 LRAPGDVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKM 183
            R+   V+GYG+ RI  FF+K MW EGFTI G  +    +LWP ++  +CD++EEY+  M
Sbjct: 125 ARSETGVSGYGVARIASFFNKQMWSEGFTITGSPLNDFRKLWPQHHLNYCDIVEEYEEHM 184

Query: 184 KSLAEQITHSIFNYLKISDA-ANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPH 243
           K LA ++     N L +S+    W   +     A    ALQLN YP CP+P R MGLA H
Sbjct: 185 KKLASKLMWLALNSLGVSEEDIEWASLSSDLNWA--QAALQLNHYPVCPEPDRAMGLAAH 244

Query: 244 TDTFLLTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVV 303
           TD+ LLTIL+Q  T GLQVFRD  GWV V P PG+LV+NVGDLFHILSNG F +VLHR  
Sbjct: 245 TDSTLLTILYQNNTAGLQVFRDDLGWVTVPPFPGSLVVNVGDLFHILSNGLFKSVLHRAR 304

Query: 304 VDPTRRRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMI 354
           V+ TR RLS+A+ +GP  D  +SP   L  P ESP Y+SV  K+Y+R KA +   ALSMI
Sbjct: 305 VNQTRARLSVAFLWGPQSDIKISPVPKLVSPVESPLYQSVTWKEYLRTKATHFNKALSMI 353

BLAST of Chy3G061460 vs. TAIR 10
Match: AT1G80340.1 (gibberellin 3-oxidase 2 )

HSP 1 Score: 307.8 bits (787), Expect = 1.2e-83
Identity = 161/356 (45.22%), Postives = 217/356 (60.96%), Query Frame = 0

Query: 2   TTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIPL 61
           +TLS  +  HP IH     P DF SL   PDS+ W      TP        +  D ++PL
Sbjct: 3   STLSDVFRSHP-IHIPLSNPPDFKSL---PDSYTW------TPKDDLLFSASASDETLPL 62

Query: 62  IDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAPG 121
           IDL+D+   +L+ +AC TWG FQ+ NH VP  L++  E  T  LF LP+ RKLKA R+  
Sbjct: 63  IDLSDIHVATLVGHACTTWGAFQITNHGVPSRLLDDIEFLTGSLFRLPVQRKLKAARSEN 122

Query: 122 DVTGYGLPRITPFFSKYMWHEGFTIMGPSIGHASQLWPSNYQPFCDVMEEYQRKMKSLAE 181
            V+GYG+ RI  FF+K MW EGFT++G  +    +LWPS++  +C+++EEY+  M+ LA 
Sbjct: 123 GVSGYGVARIASFFNKKMWSEGFTVIGSPLHDFRKLWPSHHLKYCEIIEEYEEHMQKLAA 182

Query: 182 QITHSIFNYLKISDA-ANWLHTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFL 241
           ++       L + +    W     ++        +QLN YP+CP+P R MGLA HTD+ L
Sbjct: 183 KLMWFALGSLGVEEKDIQW--AGPNSDFQGTQAVIQLNHYPKCPEPDRAMGLAAHTDSTL 242

Query: 242 LTILHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTR 301
           +TIL+Q  T GLQVFRD  GWV   PVPG+LV+NVGDL HIL+NG FP+VLHR  V+  R
Sbjct: 243 MTILYQNNTAGLQVFRDDVGWVTAPPVPGSLVVNVGDLLHILTNGIFPSVLHRARVNHVR 302

Query: 302 RRLSMAYFYGPPPDFCVSP---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIR 354
            R SMAY +GPP D  +SP   L DP +SP Y S+  K Y+  KA +   +LS+IR
Sbjct: 303 SRFSMAYLWGPPSDIMISPLPKLVDPLQSPLYPSLTWKQYLATKATHFNQSLSIIR 346

BLAST of Chy3G061460 vs. TAIR 10
Match: AT1G80330.1 (gibberellin 3-oxidase 4 )

HSP 1 Score: 291.6 bits (745), Expect = 8.6e-79
Identity = 150/338 (44.38%), Postives = 216/338 (63.91%), Query Frame = 0

Query: 26  SLRTIPDSHDW---FNSSLETPSSSSCHHHTNVDVSIPLIDLTDLDAISLINNACETWGV 85
           SL+T+P+S  W      SL  PSS+       V+ SIP+IDL++ D  +LI +A +TWG 
Sbjct: 15  SLQTLPESFTWKLTAADSLLRPSSAV--SFDAVEESIPVIDLSNPDVTTLIGDASKTWGA 74

Query: 86  FQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHE 145
           FQ+ NH +   L++  E  ++ LFD+P  RKL+A  +   V+GYG PRI+PFF K MW E
Sbjct: 75  FQIANHGISQKLLDDIESLSKTLFDMPSERKLEAASSDKGVSGYGEPRISPFFEKKMWSE 134

Query: 146 GFTIMGPSI-GHASQLWPSNYQPFCDVMEEYQRKMKSLAEQITHSIFNYLKIS-DAANWL 205
           GFTI   S   H + LWP ++  +C +++EY  +M+ LA ++ + I   L ++ +   W 
Sbjct: 135 GFTIADDSYRNHFNTLWPHDHTKYCGIIQEYVDEMEKLASRLLYCILGSLGVTVEDIEWA 194

Query: 206 HTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTDTFLLTILHQTRTCGLQVFRDGSG 265
           H    +G+     A++LN YP CP+P R MGLA HTD+ +LTILHQ+ T GLQVFR+ SG
Sbjct: 195 HKLEKSGSKVGRGAIRLNHYPVCPEPERAMGLAAHTDSTILTILHQSNTGGLQVFREESG 254

Query: 266 WVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRVVVDPTRRRLSMAYFY-GPPPDFCVSP 325
           WV V P PG LV+N+GDLFHILSNG+ P+V+HR  V+ TR R+S+AY + GP  D  ++P
Sbjct: 255 WVTVEPAPGVLVVNIGDLFHILSNGKIPSVVHRAKVNHTRSRISIAYLWGGPAGDVQIAP 314

Query: 326 ---LYDPPESPCYRSVMVKDYVRLKAKNLENALSMIRL 355
              L  P E   YRS+  K+Y+++K +  + A+  IR+
Sbjct: 315 ISKLTGPAEPSLYRSITWKEYLQIKYEVFDKAMDAIRV 350

BLAST of Chy3G061460 vs. TAIR 10
Match: AT4G21690.1 (gibberellin 3-oxidase 3 )

HSP 1 Score: 285.4 bits (729), Expect = 6.2e-77
Identity = 147/360 (40.83%), Postives = 222/360 (61.67%), Query Frame = 0

Query: 1   MTTLSQTYLDHPLIHHHHIVPLDFDSLRTIPDSHDWFNSSLETPSSSSCHHHTNVDVSIP 60
           M++++Q + ++P ++   I+PLDF + +T+PDSH W     ET S             IP
Sbjct: 1   MSSVTQLFKNNP-VNRDRIIPLDFTNTKTLPDSHVWSKPEPETTSG-----------PIP 60

Query: 61  LIDLTDLDAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDLPMTRKLKALRAP 120
           +I L++ +   L+  ACE WGVF + +H V  SL+   + + +RLF LPM RK+ A+R+P
Sbjct: 61  VISLSNPEEHGLLRQACEEWGVFHITDHGVSHSLLHNVDCQMKRLFSLPMHRKILAVRSP 120

Query: 121 GDVTGYGLPRITPFFSKYMWHEGFTIMGPSI-GHASQLWPSNYQPFCDVMEEYQRKMKSL 180
            + TGYG+ RI+ F+ K MW EGF++MG S+  HA+ LWP ++  FC+VMEEYQ+ M  L
Sbjct: 121 DESTGYGVVRISMFYDKLMWSEGFSVMGSSLRRHATLLWPDDHAEFCNVMEEYQKAMDDL 180

Query: 181 AEQITHSIFNYLKIS-DAANWL-HTAGSAGAAACSTALQLNCYPRCPDPTRVMGLAPHTD 240
           + ++   +   L ++ +   WL      +G  +  + LQLN YP CPDP   MGLAPHTD
Sbjct: 181 SHRLISMLMGSLGLTHEDLGWLVPDKTGSGTDSIQSFLQLNSYPVCPDPHLAMGLAPHTD 240

Query: 241 TFLLTILHQTRTCGLQV---FRDGSGWVPVAPVPGALVLNVGDLFHILSNGRFPNVLHRV 300
           + LLTIL+Q    GL++     +GS W+ V P+ G+LV+ +GDL HI+SNG+F + +HR 
Sbjct: 241 SSLLTILYQGNIPGLEIESPQEEGSRWIGVEPIEGSLVVIMGDLSHIISNGQFRSTMHRA 300

Query: 301 VVDPTRRRLSMAYFYGPPPDFCVSPL-YDPPESPCYRSVMVKDYVRLKAKNLENALSMIR 354
           VV+ T  R+S AYF GPP +  + PL  D    P YR ++ ++Y+  KA +   AL++ R
Sbjct: 301 VVNKTHHRVSAAYFAGPPKNLQIGPLTSDKNHPPIYRRLIWEEYLAAKATHFNKALTLFR 348

BLAST of Chy3G061460 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 174.9 bits (442), Expect = 1.2e-43
Identity = 103/299 (34.45%), Postives = 160/299 (53.51%), Query Frame = 0

Query: 53  TNVDVSIPLIDLTDL----DAISLINNACETWGVFQLINHNVPMSLIERAEGETRRLFDL 112
           ++  + IP++D+ D+    + + L+ +ACE WG FQ++NH V  SL+ER  G  R  F+L
Sbjct: 42  SDAGIEIPVLDMNDVWGKPEGLRLVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFEL 101

Query: 113 PMTRKLKALRAPGDVTGYGLPRITPFFSKYMWHEGFTI--MGPSIGHASQLWPSNYQPFC 172
           P+  K K   +P    GYG        +K  W + F +  +  SI + S+ WPS      
Sbjct: 102 PLEEKRKYANSPDTYEGYGSRLGVVKDAKLDWSDYFFLNYLPSSIRNPSK-WPSQPPKIR 161

Query: 173 DVMEEYQRKMKSLAEQITHSIFNYLKISDAANWLHTAGSAGAAACSTALQLNCYPRCPDP 232
           +++E+Y  +++ L E++T ++   L +    N L  A   G      +L+ N YP+CP P
Sbjct: 162 ELIEKYGEEVRKLCERLTETLSESLGLK--PNKLMQA-LGGGDKVGASLRTNFYPKCPQP 221

Query: 233 TRVMGLAPHTDTFLLTI-LHQTRTCGLQVFRDGSGWVPVAPVPGALVLNVGDLFHILSNG 292
              +GL+ H+D   +TI L   +  GLQV R G GWV +  VP AL++N+GD   ILSNG
Sbjct: 222 QLTLGLSSHSDPGGITILLPDEKVAGLQV-RRGDGWVTIKSVPNALIVNIGDQLQILSNG 281

Query: 293 RFPNVLHRVVVDPTRRRLSMAYFYGPPPDFCVSP-----------LYDPPESPCYRSVM 334
            + +V H+V+V+    R+S+A+FY P  D  V P           LY P     YRS++
Sbjct: 282 IYKSVEHQVIVNSGMERVSLAFFYNPRSDIPVGPIEELVTANRPALYKPIRFDEYRSLI 335

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q391032.4e-8647.37Gibberellin 3-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA3OX1 PE=1 ... [more]
O246485.4e-8647.22Gibberellin 3-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=LE PE=1 SV=1[more]
Q9ZT841.6e-8245.22Gibberellin 3-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA3OX2 PE=1 ... [more]
Q9C9711.2e-7744.38Gibberellin 3-beta-dioxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=GA3OX4 PE=1 ... [more]
Q9SVS88.7e-7640.83Gibberellin 3-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA3OX3 PE=2 ... [more]
Match NameE-valueIdentityDescription
S0BE233.7e-20296.33GA 3-oxidase OS=Cucumis sativus OX=3659 GN=GA3ox2 PE=2 SV=1[more]
A0A0A0LPF23.7e-20296.33Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G... [more]
A0A5A7VCQ31.9e-19090.68Gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3BCU47.2e-19090.45gibberellin 3-beta-dioxygenase 1-like OS=Cucumis melo OX=3656 GN=LOC103488262 PE... [more]
I0J1I81.5e-16379.27Gibberellin 3-oxidase OS=Cucurbita maxima OX=3661 GN=ga3ox4 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
NP_001274405.12.58e-25896.33gibberellin 3-beta-dioxygenase 1-like [Cucumis sativus] >CCD28483.1 GA 3-oxidase... [more]
KGN62899.15.29e-25896.33hypothetical protein Csa_021969 [Cucumis sativus][more]
KAA0064970.16.19e-24390.68gibberellin 3-beta-dioxygenase 1-like [Cucumis melo var. makuwa][more]
XP_008445137.16.76e-24290.45PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Cucumis melo][more]
XP_038886716.11.33e-22184.59gibberellin 3-beta-dioxygenase 1-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G15550.11.7e-8747.37gibberellin 3-oxidase 1 [more]
AT1G80340.11.2e-8345.22gibberellin 3-oxidase 2 [more]
AT1G80330.18.6e-7944.38gibberellin 3-oxidase 4 [more]
AT4G21690.16.2e-7740.83gibberellin 3-oxidase 3 [more]
AT2G38240.11.2e-4334.452-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 59..149
e-value: 1.5E-16
score: 61.2
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 19..348
e-value: 2.7E-78
score: 265.6
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 214..311
e-value: 2.0E-26
score: 92.4
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 12..348
NoneNo IPR availablePANTHERPTHR47990:SF105GIBBERELLIN 2-BETA-DIOXYGENASEcoord: 12..348
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 17..344
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 211..311
score: 13.073103

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G061460.1Chy3G061460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009686 gibberellin biosynthetic process
biological_process GO:0009416 response to light stimulus
molecular_function GO:0016707 gibberellin 3-beta-dioxygenase activity
molecular_function GO:0046872 metal ion binding