Chy3G053330 (gene) Cucumber (hystrix) v1

Overview


Name	Chy3G053330
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	flavonol synthase/flavanone 3-hydroxylase
Location	chrH03: 4790950 .. 4792141 (-)
RNA-Seq Expression	Chy3G053330
Synteny	Chy3G053330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGTTGGGAGAGTCCAAGAAGTTGCTTCCTCTCTTCTTTTCTCCGGCACTCTCCCCTCCCAGTTTGTCCGTCCGGACACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATCGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCACTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCGTGGGGAAGGAATTCTTCCAGCTCCCACCTGAGTTGAAAGAGATATACGCCAAACCCCCTGCTTCCAAATCCGTAGAAGGCTACGGAACTAATCTCCAAAAAGAAATCGATGGAAAAAGAGGTTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGTAACAAAATCAATAAATTCCTTCAAGTTCTTTCTTAATAATTTGTTATTGTACTCTGTTTTGTTTTTGGGTGCACGTGTGTTCGAGAATGCAGGGTAACGAATGAAGAGTACAGAAAGCATTTACATGGGGTTGTTGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAGTTGGTGTATTTACTGAAAATAAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCAGCCATTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGGCATTGGTATGATGTTAATTACATTCCTAATGCCCTTGTCATCCATATCGGAGATCAAATTGAGGTAAAGTGTTTCTTGAATCCAAGAGATGTTTGAATTTTTGAGTTGCGTCGGAAATGGCTGAATTAGAAGTTTGTTGTGTTATGGGGGCAGATTCTGAGTAATGGAAAGTACAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTCTTGGAGCCACCGCCAGAGTTTGAAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGGCAAGATATAAAACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAA

mRNA sequence

ATGGAGGTTGGGAGAGTCCAAGAAGTTGCTTCCTCTCTTCTTTTCTCCGGCACTCTCCCCTCCCAGTTTGTCCGTCCGGACACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATCGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCACTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCGTGGGGAAGGAATTCTTCCAGCTCCCACCTGAGTTGAAAGAGATATACGCCAAACCCCCTGCTTCCAAATCCGTAGAAGGCTACGGAACTAATCTCCAAAAAGAAATCGATGGAAAAAGAGGTTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGGTAACGAATGAAGAGTACAGAAAGCATTTACATGGGGTTGTTGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAGTTGGTGTATTTACTGAAAATAAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCAGCCATTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGGCATTGGTATGATGTTAATTACATTCCTAATGCCCTTGTCATCCATATCGGAGATCAAATTGAGATTCTGAGTAATGGAAAGTACAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTCTTGGAGCCACCGCCAGAGTTTGAAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGGCAAGATATAAAACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAA

Coding sequence (CDS)

ATGGAGGTTGGGAGAGTCCAAGAAGTTGCTTCCTCTCTTCTTTTCTCCGGCACTCTCCCCTCCCAGTTTGTCCGTCCGGACACCGAACAGCCCGGAATCACTACCGTCAAAGGCCAAGTCCTTGCTGTCCCTGTCATCGATTTCACCAACCCAGATGACGATTCTGTTCTCACAGCCATTCACGACGCTTCACTCAATTGGGGCATGTTTCAAATCATTAACCACCAGATCCCAAATGAAGCAATAACCCATTTGCAAGCCGTGGGGAAGGAATTCTTCCAGCTCCCACCTGAGTTGAAAGAGATATACGCCAAACCCCCTGCTTCCAAATCCGTAGAAGGCTACGGAACTAATCTCCAAAAAGAAATCGATGGAAAAAGAGGTTGGGTCGACCATTTGTTCCACATCATTTGGCCTCCTTCTTCCATTAATTACAATTTCTGGCCTCAAAATCCCCCTTCTTACAGGGTAACGAATGAAGAGTACAGAAAGCATTTACATGGGGTTGTTGAGAAGCTGTTCAGAAGCCTCTCGCTGGGTTTGGGGCTTGAAGGGCATGAACTAAAAGAGCGAGTGGGTGGAGATGAGTTGGTGTATTTACTGAAAATAAATTACTATCCGCCATGTCCACGCCCTGATCTGGCTCTTGGAGTCCCTGCTCATACCGACATGTCAGCCATTACGATTCTTGTGCCGAATGAAGTTCAAGGACTTCAGGCGTTTAGAGATGGGCATTGGTATGATGTTAATTACATTCCTAATGCCCTTGTCATCCATATCGGAGATCAAATTGAGATTCTGAGTAATGGAAAGTACAAGAGTGTGTTTCATAGGACGACTGTGAACAAGGAGAAGACGAGGATATCGTGGCCGGTGTTCTTGGAGCCACCGCCAGAGTTTGAAGTTGGGCCTCATCCGAAATTGGTCGATGAGGAAAATCCGGCAAGATATAAAACTAAGAAGTACAGTGAATATGCATATTGTAAGCTGAACAAGATTCCACAGTAA

Protein sequence

MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ*

Homology

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BLAST of Chy3G053330 vs. ExPASy Swiss-Prot
Match: Q07512 (Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 1.4e-144
Identity = 232/336 (69.05%), Postives = 278/336 (82.74%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEV RVQ +AS      T+PS+++R + EQP  TT+ G VL VPVID  +PD++ ++  I
Sbjct: 13  MEVARVQAIASLSKCMDTIPSEYIRSENEQPAATTLHGVVLQVPVIDLRDPDENKMVKLI 72

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQ-LPPELKEIYAKPPASKSVEGYGTNL 120
            DAS  WG+FQ+INH IP+EAI  LQ VGKEFF+ +P E KE+ AK P S  +EGYGT+L
Sbjct: 73  ADASKEWGIFQLINHGIPDEAIADLQKVGKEFFEHVPQEEKELIAKTPGSNDIEGYGTSL 132

Query: 121 QKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSL 180
           QKE++GK+GWVDHLFH IWPPS++NY +WP+NPPSYR  NEEY K +  VV+++F+SLSL
Sbjct: 133 QKEVEGKKGWVDHLFHKIWPPSAVNYRYWPKNPPSYREANEEYGKRMREVVDRIFKSLSL 192

Query: 181 GLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQ 240
           GLGLEGHE+ E  GGDE+VYLLKINYYPPCPRPDLALGV AHTDMS ITILVPNEVQGLQ
Sbjct: 193 GLGLEGHEMIEAAGGDEIVYLLKINYYPPCPRPDLALGVVAHTDMSYITILVPNEVQGLQ 252

Query: 241 AFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPE 300
            F+DGHWYDV YIPNAL++HIGDQ+EILSNGKYKSV+HRTTVNK+KTR+SWPVFLEPP E
Sbjct: 253 VFKDGHWYDVKYIPNALIVHIGDQVEILSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPSE 312

Query: 301 FEVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 336
            EVGP PKL+ E NP ++KTKKY +Y YCKLNK+PQ
Sbjct: 313 HEVGPIPKLLSEANPPKFKTKKYKDYVYCKLNKLPQ 348

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BLAST of Chy3G053330 vs. ExPASy Swiss-Prot
Match: Q9M547 (Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellianum OX=52518 GN=FLS PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.0e-142
Identity = 229/335 (68.36%), Postives = 277/335 (82.69%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEV RVQE+AS      T+P++++R + EQP I+TV G VL VPVID ++ D+  ++  +
Sbjct: 1   MEVQRVQEIASLSKVIDTIPAEYIRSENEQPVISTVHGVVLEVPVIDLSDSDEKKIVGLV 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
            +AS  WG+FQ++NH IPNE I  LQ VGK FF+LP E KE+ AKP  S+S+EGYGT LQ
Sbjct: 61  SEASKEWGIFQVVNHGIPNEVIRKLQEVGKHFFELPQEEKELIAKPEGSQSIEGYGTRLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFH IWPPS+INY FWP+NPP+YR  NEEY K L  VV+ LF+ LSLG
Sbjct: 121 KEVDGKKGWVDHLFHKIWPPSAINYQFWPKNPPAYREANEEYAKRLQLVVDNLFKYLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           L LE +  K+  GGD+LVYL+KINYYPPCPRPDLALGV AHTDMSAIT+LVPNEV GLQ 
Sbjct: 181 LDLEPNSFKDGAGGDDLVYLMKINYYPPCPRPDLALGV-AHTDMSAITVLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           ++DGHWYD  YIPNAL++HIGDQ+EI+SNGKYKSV+HRTTVNKEKTR+SWPVFLEPPP+ 
Sbjct: 241 YKDGHWYDCKYIPNALIVHIGDQVEIMSNGKYKSVYHRTTVNKEKTRMSWPVFLEPPPDH 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 336
           EVGP PKLV+EENPA++KTKKY +YAYCKLNK+PQ
Sbjct: 301 EVGPIPKLVNEENPAKFKTKKYKDYAYCKLNKLPQ 334

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BLAST of Chy3G053330 vs. ExPASy Swiss-Prot
Match: Q9XHG2 (Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.1e-141
Identity = 230/323 (71.21%), Postives = 269/323 (83.28%), Query Frame = 0


Query: 17  GTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQ 76
           GT+P++F+R + EQPGITTV G+VL VP+IDF++PD++ ++  I +AS NWGM+QI+NH 
Sbjct: 15  GTIPAEFIRSENEQPGITTVHGKVLEVPIIDFSDPDEEKLIVQITEASSNWGMYQIVNHD 74

Query: 77  IPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQKEID----GKRGWVDH 136
           IP+E I+ LQAVGKEFF+LP E KE YAKPP S S+EGYGT L KEI      K+GWVD+
Sbjct: 75  IPSEVISKLQAVGKEFFELPQEEKEAYAKPPDSASIEGYGTKLFKEISEGDTTKKGWVDN 134

Query: 137 LFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLGLGLEGHELKERV 196
           LF+ IWPPS +NY FWP+NPPSYR  NEEY KHLH VVEKLFR LSLGLGLEG ELK+  
Sbjct: 135 LFNKIWPPSVVNYQFWPKNPPSYREANEEYAKHLHNVVEKLFRLLSLGLGLEGQELKKAA 194

Query: 197 GGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQAFRDGHWYDVNYI 256
           GGD L YLLKINYYPPCPRPDLALGV AHTDMS +TILVPN+VQGLQA +DG WYDV YI
Sbjct: 195 GGDNLEYLLKINYYPPCPRPDLALGVVAHTDMSTVTILVPNDVQGLQACKDGRWYDVKYI 254

Query: 257 PNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEE 316
           PNALVIHIGDQ+EI+SNGKY SV HRTTVNK+KTRISWPVFLEPP +  VGPHP+LV+  
Sbjct: 255 PNALVIHIGDQMEIMSNGKYTSVLHRTTVNKDKTRISWPVFLEPPADHVVGPHPQLVNAV 314

Query: 317 NPARYKTKKYSEYAYCKLNKIPQ 336
           N  +YKTKKY +Y YCK+NK+PQ
Sbjct: 315 NQPKYKTKKYGDYVYCKINKLPQ 337

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BLAST of Chy3G053330 vs. ExPASy Swiss-Prot
Match: Q7XZQ6 (Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS PE=1 SV=1)

HSP 1 Score: 498.0 bits (1281), Expect = 8.1e-140
Identity = 225/337 (66.77%), Postives = 275/337 (81.60%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDF--TNPDDDSVLT 60
           MEV RVQ ++       T+PS+++R ++EQP +TT++G VL VPVID   +N  +++++ 
Sbjct: 1   MEVERVQAISKMSRCMDTIPSEYIRSESEQPAVTTMQGVVLQVPVIDLGSSNNTEENLVE 60

Query: 61  AIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTN 120
            I +AS  WG+FQ++NH IP++AI  LQ VGKEFF+LP + KE+ AKP   + VEGYGT 
Sbjct: 61  LIAEASREWGIFQVVNHGIPDDAIAKLQKVGKEFFELPQQEKEVIAKPEGYQGVEGYGTK 120

Query: 121 LQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLS 180
           LQKE+ GK+GWVDHLFHIIWP S++NYNFWP NPP YR  NEEY   L GVV+KLF +LS
Sbjct: 121 LQKELGGKKGWVDHLFHIIWPKSAVNYNFWPNNPPLYREANEEYAVALRGVVDKLFEALS 180

Query: 181 LGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGL 240
           LG+GLE HELK+  GGD+L+Y+LKINYYPPCPRPDLALGV AHTDMSAITILVPNEVQGL
Sbjct: 181 LGIGLEKHELKKASGGDDLIYMLKINYYPPCPRPDLALGVVAHTDMSAITILVPNEVQGL 240

Query: 241 QAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPP 300
           Q  +D HWYDV YIPNAL+IHIGDQIEI+SNGKYKSV+HRTTVNK+KTR+SWPVFLEPPP
Sbjct: 241 QVHKDDHWYDVKYIPNALIIHIGDQIEIMSNGKYKSVYHRTTVNKDKTRMSWPVFLEPPP 300

Query: 301 EFEVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 336
           E   GP  KL+ +ENPA++KTKKY +Y YCKLNK+PQ
Sbjct: 301 ELLTGPISKLITDENPAKFKTKKYKDYVYCKLNKLPQ 337

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BLAST of Chy3G053330 vs. ExPASy Swiss-Prot
Match: Q41452 (Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 1.4e-136
Identity = 224/338 (66.27%), Postives = 274/338 (81.07%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDD--SVLT 60
           MEV RVQ ++S      T+PS+++R + EQP  TT++G VL VPVID +N DDD   ++ 
Sbjct: 15  MEVARVQAISSITKCMDTIPSEYIRSENEQPAATTLQGVVLEVPVIDISNVDDDEEKLVK 74

Query: 61  AIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFF-QLPPELKEIYAKPPASKSVEGYGT 120
            I +AS  WG+FQ+INH IP+E I +LQ VGKEFF ++P E KE+ AK P ++S+EGYGT
Sbjct: 75  EIVEASKEWGIFQVINHGIPDEVIENLQKVGKEFFEEVPQEEKELIAKKPGAQSLEGYGT 134

Query: 121 NLQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSL 180
           +LQKEI+GK+GWVDHLFH IWPPS+INY +WP+NPPSYR  NEEY K L  V + +FRSL
Sbjct: 135 SLQKEIEGKKGWVDHLFHKIWPPSAINYRYWPKNPPSYREANEEYAKWLRKVADGIFRSL 194

Query: 181 SLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQG 240
           SLGLGLEGHE+ E  G +++VY+LKINYYPPCPRPDLALGV AHTDMS IT+LVPNEV  
Sbjct: 195 SLGLGLEGHEMMEAAGSEDIVYMLKINYYPPCPRPDLALGVVAHTDMSYITLLVPNEV-- 254

Query: 241 LQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPP 300
            Q F+DGHWYDVNYIPNA+++HIGDQ+EILSNGKYKSV+HRTTVNK KTR+SWPVFLEP 
Sbjct: 255 -QVFKDGHWYDVNYIPNAIIVHIGDQVEILSNGKYKSVYHRTTVNKYKTRMSWPVFLEPS 314

Query: 301 PEFEVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 336
            E EVGP P L++E NP ++KTKKY +Y YCKLNK+PQ
Sbjct: 315 SEHEVGPIPNLINEANPPKFKTKKYKDYVYCKLNKLPQ 349

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BLAST of Chy3G053330 vs. ExPASy TrEMBL
Match: A0A0A0K885 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G040540 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 1.7e-196
Identity = 329/335 (98.21%), Postives = 331/335 (98.81%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPE+KEIYAKPPASKSVEGYGTNLQ
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYR  NEEYRKHLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVDE+ PARYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

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BLAST of Chy3G053330 vs. ExPASy TrEMBL
Match: A0A5D3CHN5 (Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1827G00220 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 5.9e-194
Identity = 323/335 (96.42%), Postives = 331/335 (98.81%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFF+LPPE KEIYAKPPASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRV NEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 336
            VGPHPKLVDEENPA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Chy3G053330 vs. ExPASy TrEMBL
Match: A0A1S3BCS7 (flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC103488630 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 5.9e-194
Identity = 323/335 (96.42%), Postives = 331/335 (98.81%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFF+LPPE KEIYAKPPASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRV NEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 336
            VGPHPKLVDEENPA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Chy3G053330 vs. ExPASy TrEMBL
Match: A0A6J1FDW3 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111444648 PE=3 SV=1)

HSP 1 Score: 629.0 bits (1621), Expect = 1.1e-176
Identity = 288/335 (85.97%), Postives = 312/335 (93.13%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS LFSG+LP++FVRPDTEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH IPNEAI HLQAVGK FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KE+DG++GWVDHLFHIIWPPS+IN+ FWPQNPPSYR  NEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGRKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP +YKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Chy3G053330 vs. ExPASy TrEMBL
Match: A0A6J1JY72 (flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111489355 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.3e-176
Identity = 287/335 (85.67%), Postives = 312/335 (93.13%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS LFSG++P++FVRPD EQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSIPAEFVRPDPEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH IPNEAI HLQAVGK FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYRV NEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRVANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELK+  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKQSAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRARISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 336
           EVGPHPKLVD++NP +YKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Chy3G053330 vs. NCBI nr
Match: XP_004140862.1 (flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothetical protein Csa_005329 [Cucumis sativus])

HSP 1 Score: 689 bits (1779), Expect = 1.47e-250
Identity = 329/335 (98.21%), Postives = 331/335 (98.81%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPE+KEIYAKPPASKSVEGYGTNLQ
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPEMKEIYAKPPASKSVEGYGTNLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYR  NEEYRKHLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRAANEEYRKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSV HRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 335
           EVGPHPKLVDE+ PARYKTKKYSEYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDEKIPARYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Chy3G053330 vs. NCBI nr
Match: XP_008445671.1 (PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa] >TYK11311.1 flavonol synthase/flavanone 3-hydroxylase [Cucumis melo var. makuwa])

HSP 1 Score: 681 bits (1757), Expect = 3.33e-247
Identity = 323/335 (96.42%), Postives = 331/335 (98.81%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           M+VGRVQEVASSLLFSGTLPS+FVRP+TEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI
Sbjct: 1   MDVGRVQEVASSLLFSGTLPSEFVRPETEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
           HDASLNWGMFQIINHQIPNEAITHLQAVGKEFF+LPPE KEIYAKPPASKSVEGYGT L+
Sbjct: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFELPPEEKEIYAKPPASKSVEGYGTKLE 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRV NEEYRK+LHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVANEEYRKNLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSA+TILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAVTILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 335
            VGPHPKLVDEENPA+YKTKKYSEYAYCKLNKIPQ
Sbjct: 301 VVGPHPKLVDEENPAKYKTKKYSEYAYCKLNKIPQ 335

[Close]

BLAST of Chy3G053330 vs. NCBI nr
Match: XP_038885635.1 (flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida])

HSP 1 Score: 644 bits (1661), Expect = 1.44e-232
Identity = 302/334 (90.42%), Postives = 319/334 (95.51%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS+L+S TLP++FVR +TEQPGITTVKGQVLAVPVIDFT+PDDDSVL AI
Sbjct: 1   MEVGRVQDIASSVLYSDTLPAEFVRSETEQPGITTVKGQVLAVPVIDFTDPDDDSVLRAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH+IPNEAITHLQAVGKEFF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHEIPNEAITHLQAVGKEFFELPPEEKEVYAKPPGSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KEIDGK+GWVDHLFHIIWPPS+INYNFWPQNPPSYR  NEEY KHLHGVVEKLFRSLSLG
Sbjct: 121 KEIDGKKGWVDHLFHIIWPPSAINYNFWPQNPPSYRAANEEYSKHLHGVVEKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKER GG+ELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA
Sbjct: 181 LGLEGHELKERAGGEELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDG WYDVNYIPNALVIHIGDQ+EILSNGKYKSVFHRT VNKEKTRISWPVFLEPPPEF
Sbjct: 241 FRDGQWYDVNYIPNALVIHIGDQMEILSNGKYKSVFHRTRVNKEKTRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIP 334
           EVGPHPKLVDE+NP +YKTKKYSEYAYCKLNKIP
Sbjct: 301 EVGPHPKLVDEKNPPKYKTKKYSEYAYCKLNKIP 334

[Close]

BLAST of Chy3G053330 vs. NCBI nr
Match: KAG7016505.1 (Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625 bits (1611), Expect = 5.99e-225
Identity = 289/335 (86.27%), Postives = 312/335 (93.13%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS LFSG+LP++FVRPDTEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIKAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH IPNEAI HLQAVGK FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYR  NEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 335
           EVGPHPKLVD++NP +YKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Chy3G053330 vs. NCBI nr
Match: XP_023551148.1 (flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 625 bits (1611), Expect = 5.99e-225
Identity = 289/335 (86.27%), Postives = 312/335 (93.13%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEVGRVQ++ASS LFSG+LP++FVRPDTEQPGITTVKGQ+LAVPVIDF NPD+DSV+ AI
Sbjct: 1   MEVGRVQDIASSALFSGSLPAEFVRPDTEQPGITTVKGQLLAVPVIDFKNPDEDSVIRAI 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
            DAS NWGMFQIINH IPNEAI HLQAVGK FF+LPPE KE+YAKPP SKSVEGYGT LQ
Sbjct: 61  DDASRNWGMFQIINHDIPNEAIAHLQAVGKAFFELPPEEKEVYAKPPDSKSVEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           KE+DGK+GWVDHLFHIIWPPS+IN+ FWPQNPPSYR  NEEYRKHLHGVV+KLFRSLSLG
Sbjct: 121 KEVDGKKGWVDHLFHIIWPPSAINFKFWPQNPPSYRAANEEYRKHLHGVVDKLFRSLSLG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGLEGHELKE  GGD+LVYLLKINYYPPCP PDLALGVPAHTDMS ITILVPN+VQGLQA
Sbjct: 181 LGLEGHELKESAGGDKLVYLLKINYYPPCPCPDLALGVPAHTDMSTITILVPNDVQGLQA 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           FRDGHWY+VNYIPNALVIHIGDQIEILSNGKYKSV HRTTV K++ RISWPVFLEPPPEF
Sbjct: 241 FRDGHWYNVNYIPNALVIHIGDQIEILSNGKYKSVLHRTTVKKDRVRISWPVFLEPPPEF 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIPQ 335
           EVGPHPKLVD++NP +YKTKKY EYAYCKLNKIPQ
Sbjct: 301 EVGPHPKLVDQDNPPKYKTKKYGEYAYCKLNKIPQ 335

[Close]

BLAST of Chy3G053330 vs. TAIR 10
Match: AT5G08640.1 (flavonol synthase 1 )

HSP 1 Score: 438.7 bits (1127), Expect = 4.1e-123
Identity = 197/334 (58.98%), Postives = 253/334 (75.75%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEV RVQ+++SS L +  +P +F+R + EQP ITT +G   A+PV+D ++PD++SV  A+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
             AS  WG+FQ++NH IP E I  LQ VG++FF+LP   KE  AKP  SK +EGYGT LQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           K+ +GK+ WVDHLFH IWPPS +NY FWP+NPP YR  NEEY  H+  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGL+   LKE +GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIP 335
            VGP P+L  ++NP ++K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of Chy3G053330 vs. TAIR 10
Match: AT5G08640.2 (flavonol synthase 1 )

HSP 1 Score: 438.7 bits (1127), Expect = 4.1e-123
Identity = 197/334 (58.98%), Postives = 253/334 (75.75%), Query Frame = 0


Query: 1   MEVGRVQEVASSLLFSGTLPSQFVRPDTEQPGITTVKGQVLAVPVIDFTNPDDDSVLTAI 60
           MEV RVQ+++SS L +  +P +F+R + EQP ITT +G   A+PV+D ++PD++SV  A+
Sbjct: 1   MEVERVQDISSSSLLTEAIPLEFIRSEKEQPAITTFRGPTPAIPVVDLSDPDEESVRRAV 60

Query: 61  HDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELKEIYAKPPASKSVEGYGTNLQ 120
             AS  WG+FQ++NH IP E I  LQ VG++FF+LP   KE  AKP  SK +EGYGT LQ
Sbjct: 61  VKASEEWGLFQVVNHGIPTELIRRLQDVGRKFFELPSSEKESVAKPEDSKDIEGYGTKLQ 120

Query: 121 KEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNEEYRKHLHGVVEKLFRSLSLG 180
           K+ +GK+ WVDHLFH IWPPS +NY FWP+NPP YR  NEEY  H+  + E L   LS G
Sbjct: 121 KDPEGKKAWVDHLFHRIWPPSCVNYRFWPKNPPEYREVNEEYAVHVKKLSETLLGILSDG 180

Query: 181 LGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPAHTDMSAITILVPNEVQGLQA 240
           LGL+   LKE +GG+   Y++KINYYPPCPRPDLALGVPAHTD+S IT+LVPNEV GLQ 
Sbjct: 181 LGLKRDALKEGLGGEMAEYMMKINYYPPCPRPDLALGVPAHTDLSGITLLVPNEVPGLQV 240

Query: 241 FRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTTVNKEKTRISWPVFLEPPPEF 300
           F+D HW+D  YIP+A+++HIGDQI  LSNG+YK+V HRTTV+KEKTR+SWPVFLEPP E 
Sbjct: 241 FKDDHWFDAEYIPSAVIVHIGDQILRLSNGRYKNVLHRTTVDKEKTRMSWPVFLEPPREK 300

Query: 301 EVGPHPKLVDEENPARYKTKKYSEYAYCKLNKIP 335
            VGP P+L  ++NP ++K   + +Y+Y KLNK+P
Sbjct: 301 IVGPLPELTGDDNPPKFKPFAFKDYSYRKLNKLP 334

[Close]

BLAST of Chy3G053330 vs. TAIR 10
Match: AT5G63590.1 (flavonol synthase 3 )

HSP 1 Score: 334.0 bits (855), Expect = 1.4e-91
Identity = 152/294 (51.70%), Postives = 200/294 (68.03%), Query Frame = 0


Query: 41  LAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFFQLPPELK 100
           L +PVID +NPD++ V +A+  AS  WG+FQ++NH IP E I  L  VG EFF+LP   K
Sbjct: 12  LDIPVIDLSNPDEELVASAVVKASQEWGIFQVVNHGIPTELILRLLQVGMEFFELPETEK 71

Query: 101 EIYAKPPASKSVEGYGTNLQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPPSYRVTNE 160
           E  AKP  S  +EGY T  QK+++G+  WVDHLFH IWPPS +N+ FWP+NPP Y   NE
Sbjct: 72  EAVAKPEDSLDIEGYRTKYQKDLEGRNAWVDHLFHRIWPPSRVNHKFWPKNPPEYIEVNE 131

Query: 161 EYRKHLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPDLALGVPA 220
           EY  H+  + EK+   LS GLGL    LKE +GG+ + YL+KINYYPPCP P+L +G P 
Sbjct: 132 EYASHIKKLSEKIMEWLSEGLGLRHEALKEGLGGETIEYLMKINYYPPCPDPELVVGAPD 191

Query: 221 HTDMSAITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYKSVFHRTT 280
           HTD++ IT+LV NE  GLQAF+D  W D  Y  + +++ IGDQ   +SNGKYKSV HR  
Sbjct: 192 HTDVNGITLLVANEALGLQAFKDNQWIDAEYTTSGIIVIIGDQFLRMSNGKYKSVEHRAK 251

Query: 281 VNKEKTRISWPVFLEPPPEFEVGPHPKLV-DEENPARYKTKKYSEYAYCKLNKI 334
           ++KEKTRISWPVF+E   +   GP P+L+  +EN  ++K   Y +Y + KL K+
Sbjct: 252 MDKEKTRISWPVFVESSLDQVFGPLPELITGDENVPKFKPYVYKDYKFRKLKKL 305

[Close]

BLAST of Chy3G053330 vs. TAIR 10
Match: AT5G63600.1 (flavonol synthase 5 )

HSP 1 Score: 287.0 bits (733), Expect = 2.0e-77
Identity = 131/292 (44.86%), Postives = 189/292 (64.73%), Query Frame = 0


Query: 34  TTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFF 93
           +T+ G  + VPV+D +  D+D ++  +  AS  WG+FQ++NH IP E +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 94  QLPPELKEIYAKPPASKSVEGYGTNLQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPP 153
           +LP   KE  AK    +  EGY  N    + G   W +HLFH + PPS INY +WP+NPP
Sbjct: 84  ELPDAEKETVAK---EEDFEGYKKNY---LGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 154 SYRVTNEEYRKHLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPD 213
            YR   EEY KH+  + EK+   LS GLGL+     + +GGD   Y+L++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 214 LALGVPAHTDMSAITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEILSNGKYK 273
           L +G  AH+DM AI +L+PNEV GLQAF+D  W D++YI +A+V+ IGDQ+  ++NG+ K
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMRMTNGRLK 263

Query: 274 SVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEENPARYKTKKYSEY 326
           +V HR   +K+K RISWPVF+ P  +  VGP P+   +ENP +++T  Y++Y
Sbjct: 264 NVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 309

[Close]

BLAST of Chy3G053330 vs. TAIR 10
Match: AT5G63600.2 (flavonol synthase 5 )

HSP 1 Score: 284.6 bits (727), Expect = 1.0e-76
Identity = 131/293 (44.71%), Postives = 190/293 (64.85%), Query Frame = 0


Query: 34  TTVKGQVLAVPVIDFTNPDDDSVLTAIHDASLNWGMFQIINHQIPNEAITHLQAVGKEFF 93
           +T+ G  + VPV+D +  D+D ++  +  AS  WG+FQ++NH IP E +  LQ VG +FF
Sbjct: 24  STLGGSAVDVPVVDLSVSDEDFLVREVVKASEEWGVFQVVNHGIPTELMRQLQMVGTQFF 83

Query: 94  QLPPELKEIYAKPPASKSVEGYGTNLQKEIDGKRGWVDHLFHIIWPPSSINYNFWPQNPP 153
           +LP   KE  AK    +  EGY  N    + G   W +HLFH + PPS INY +WP+NPP
Sbjct: 84  ELPDAEKETVAK---EEDFEGYKKNY---LGGINNWDEHLFHRLSPPSIINYKYWPKNPP 143

Query: 154 SYRVTNEEYRKHLHGVVEKLFRSLSLGLGLEGHELKERVGGDELVYLLKINYYPPCPRPD 213
            YR   EEY KH+  + EK+   LS GLGL+     + +GGD   Y+L++N+YPP    +
Sbjct: 144 QYREVTEEYTKHMKRLTEKILGWLSEGLGLQRETFTQSIGGDTAEYVLRVNFYPPTQDTE 203

Query: 214 LALGVPAHTDMSAITILVPNEVQGLQAFRDGHWYDVNYIPNALVIHIGDQIEI-LSNGKY 273
           L +G  AH+DM AI +L+PNEV GLQAF+D  W D++YI +A+V+ IGDQ+ + ++NG+ 
Sbjct: 204 LVIGAAAHSDMGAIALLIPNEVPGLQAFKDEQWLDLDYIDSAVVVIIGDQLMVWMTNGRL 263

Query: 274 KSVFHRTTVNKEKTRISWPVFLEPPPEFEVGPHPKLVDEENPARYKTKKYSEY 326
           K+V HR   +K+K RISWPVF+ P  +  VGP P+   +ENP +++T  Y++Y
Sbjct: 264 KNVLHRAKSDKDKLRISWPVFVAPRADMSVGPLPEFTGDENPPKFETLIYNDY 310

The following BLAST results are available for this feature:

BLAST of Chy3G053330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q07512	1.4e-144	69.05	Flavonol synthase/flavanone 3-hydroxylase OS=Petunia hybrida OX=4102 GN=FL PE=2 ...	[more]
Q9M547	1.0e-142	68.36	Flavonol synthase/flavanone 3-hydroxylase OS=Eustoma exaltatum subsp. russellian...	[more]
Q9XHG2	1.1e-141	71.21	Flavonol synthase/flavanone 3-hydroxylase OS=Malus domestica OX=3750 GN=FLS PE=2...	[more]
Q7XZQ6	8.1e-140	66.77	Flavonol synthase/flavanone 3-hydroxylase OS=Petroselinum crispum OX=4043 GN=FLS...	[more]
Q41452	1.4e-136	66.27	Flavonol synthase/flavanone 3-hydroxylase OS=Solanum tuberosum OX=4113 PE=2 SV=1	[more]

BLAST of Chy3G053330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K885	1.7e-196	98.21	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G...	[more]
A0A5D3CHN5	5.9e-194	96.42	Flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3BCS7	5.9e-194	96.42	flavonol synthase/flavanone 3-hydroxylase OS=Cucumis melo OX=3656 GN=LOC10348863...	[more]
A0A6J1FDW3	1.1e-176	85.97	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JY72	3.3e-176	85.67	flavonol synthase/flavanone 3-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Chy3G053330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004140862.1	1.47e-250	98.21	flavonol synthase/flavanone 3-hydroxylase [Cucumis sativus] >KGN45965.1 hypothet...	[more]
XP_008445671.1	3.33e-247	96.42	PREDICTED: flavonol synthase/flavanone 3-hydroxylase [Cucumis melo] >KAA0063921....	[more]
XP_038885635.1	1.44e-232	90.42	flavonol synthase/flavanone 3-hydroxylase [Benincasa hispida]	[more]
KAG7016505.1	5.99e-225	86.27	Flavonol synthase/flavanone 3-hydroxylase, partial [Cucurbita argyrosperma subsp...	[more]
XP_023551148.1	5.99e-225	86.27	flavonol synthase/flavanone 3-hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Chy3G053330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08640.1	4.1e-123	58.98	flavonol synthase 1	[more]
AT5G08640.2	4.1e-123	58.98	flavonol synthase 1	[more]
AT5G63590.1	1.4e-91	51.70	flavonol synthase 3	[more]
AT5G63600.1	2.0e-77	44.86	flavonol synthase 5	[more]
AT5G63600.2	1.0e-76	44.71	flavonol synthase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 43..150 e-value: 3.8E-21 score: 76.0
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 201..296 e-value: 1.6E-25 score: 89.5
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 1..333 e-value: 4.5E-95 score: 320.7
None	No IPR available	PANTHER	PTHR47990:SF36	OS02G0767300 PROTEIN	coord: 6..332
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 6..332
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 17..333
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 196..296 score: 14.583344

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy3G053330.1	Chy3G053330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051555	flavonol biosynthetic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0045431	flavonol synthase activity
molecular_function	GO:0046872	metal ion binding