Chy3G051030 (gene) Cucumber (hystrix) v1

Overview
NameChy3G051030
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionRibosome-recycling factor, chloroplastic
LocationchrH03: 1155899 .. 1158039 (-)
RNA-Seq ExpressionChy3G051030
SyntenyChy3G051030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATCTTTCTCAAACGTTCCTTCTCCTACAGAAATTTCATCCTTCTCCGTAATTTTGCAAATCAAACCTGTAACTTTTCCTTCTCTGTCAACCATTTCTCCCACTCATGGACCTCCGACACCTCTCCCCGATTTCCTCTTCCTCCTCCAGATTTCATCCGAGAGACTCGTCGGGGTTTTGCTAAAGGCCGAAAATCAAGTAATGATCTTGTTGAATATATTATTTATTCTGTTCATGTTTCTCCTGCTCCGGTTTTGAATCGCTTTAATTTTTAATTTTTTATGTTGGGAACTTGGGAAGATGAATCTTCTACAAGTATGGCTGAGGCGTTGCCTGATATTCGACCCACGATTAAAGCAAACGCTAGCTCGCAAATGGAGGCATCGATAGTTGCATTATCAGGAGAATTAAGCAAGCTTCGAACGGGCAGGGCATCACCTGGTACATTCACTGTCGTATTTATCTAACATTTACACTTCATGGTTTTATTGTCTTAGCCTGCCCTTGTGATTGCTTACACTATCTCTTTCACTGCACTTACAGGAATGCTTGACCACATTATAGTAGAAACTTCAGGTGTGAAGCTGCCGTTGAATCAGATTGCGGCTGTATCGGTCTTGGATTCTAAAACATTGTCAATAAATCCTTATGATCCCAGTGTAATCAGCTTACGAAACCCTCTTCTTAAGTTATTTTATCATTTTTCTTCATCATGAGATGTGGTTAATTTCTCATGTTCGTGATTATTTGTGGTAAGGAGAACATGGGCCTGCTTTGATTGGTTTTTTGACTGCTTAAAAACATTTCTTTAAGTAATTAGAAATTCAATCCAAACATTCCATAGATTGTGCAATTCACATAGCAGAGCAAATGTTGCATGTAATCTTTAATTTTTCATCTTTGGCAACTTTTGAAATATATCAAATTGAGTTATGCTTTATTATTGTCATTATCGAAACAAGGTTCTTGTTTCTACTCTACCTGATGTGAGAATTCATAGACACTATCGACATATCTGTTAGTGTTCTTAGCTGAAAAGTGGTATTTGGAATGTCATTCTTATGATAATTACTCTTCTATGCAGACACTAAAGAATTTAGAGACGGCTATAATATCATCTCCTTTAGGTGTAGCACCAAGAGTAGACGGAGAGAGGTTAATTGCAGTCATTCCACCGTAAGTTTTCATGTTTCTCTTCCACTGTCTATATGGTTCTTTAAATGTTAAAGCATATCGTATCTGGGAGCTTGACCTTCACACTTTGACAAATATTTTATCCTGATGTTTCTCTTTTTGCTTGCTATACCAGTTTGACCAAAGAACATATCCAGGTAATCTCCAAATCATTTTGCATCTTTCCTTGTTATCCTTTCCTTGGTTCTATAATGCCTATATGGTGAACATTTCTATAGGCTATGTGTAAGCTGGTCACCAAGTGTTGCGAAGATTCCAGACAAAGCATACGACGAGCTCGTCAAAAGGTTATTTTAGCTACATCTTCACTTCATATAATTAATTTTCAGAAATGTTTGCTAGTAATCATCTAGAGCATAAAAGATCTTGTACAATTCCTATTGGTTCGTGAGGTACCTTGTTTGGTAAGCTCAATAGTTGTAGAAGTCCCAAGAGGCATTATTTTTATGACACTTGCCTCATTCATTACTTTGTATCCAAAACAATCAAGTTTCATGTTAGCTTACAAAATAACCCCAGCTTCACTTCACCTGAATGATCGTGAAGTCATTATGTTAATACCACAAACTCATAGGTGTTAGGTGTTCACAAGTGTTAGCAAGTCTTTCATATAGTCCTAACAGCCCTAGGTGTTAGGAAGTGTGTCGTAAAAGGACTTCATTTATTCACATCATTTACAGAACTGAGTTGATTTGTGGTTGATTCACAGGCCATGGATACAGTTAAAAAGATTAGCTCAAGCTACCCAAAGGATGACATAAAAAGACTGGAAAAGGAAGTGAGTTGGAGAAGTTGAATCTAATCAAATAATATTTCAATTTTGCTTTTTATTAATTAAATACTGTTGCGTTTTTGCTTTCAGGTTGATGAATTGACTAAGAAGTTTATCAAGTCAGCCGAAGAACTCTGCAAGGGAAAGGAGAAGGAGATCACTGGAGGTTAA

mRNA sequence

ATGGCTATCTTTCTCAAACGTTCCTTCTCCTACAGAAATTTCATCCTTCTCCGTAATTTTGCAAATCAAACCTGTAACTTTTCCTTCTCTGTCAACCATTTCTCCCACTCATGGACCTCCGACACCTCTCCCCGATTTCCTCTTCCTCCTCCAGATTTCATCCGAGAGACTCGTCGGGGTTTTGCTAAAGGCCGAAAATCAAATGAATCTTCTACAAGTATGGCTGAGGCGTTGCCTGATATTCGACCCACGATTAAAGCAAACGCTAGCTCGCAAATGGAGGCATCGATAGTTGCATTATCAGGAGAATTAAGCAAGCTTCGAACGGGCAGGGCATCACCTGGAATGCTTGACCACATTATAGTAGAAACTTCAGGTGTGAAGCTGCCGTTGAATCAGATTGCGGCTGTATCGGTCTTGGATTCTAAAACATTGTCAATAAATCCTTATGATCCCAGTACACTAAAGAATTTAGAGACGGCTATAATATCATCTCCTTTAGGTGTAGCACCAAGAGTAGACGGAGAGAGGTTAATTGCAGTCATTCCACCTTTGACCAAAGAACATATCCAGGCTATGTGTAAGCTGGTCACCAAGTGTTGCGAAGATTCCAGACAAAGCATACGACGAGCTCGTCAAAAGGCCATGGATACAGTTAAAAAGATTAGCTCAAGCTACCCAAAGGATGACATAAAAAGACTGGAAAAGGAAGTTGATGAATTGACTAAGAAGTTTATCAAGTCAGCCGAAGAACTCTGCAAGGGAAAGGAGAAGGAGATCACTGGAGGTTAA

Coding sequence (CDS)

ATGGCTATCTTTCTCAAACGTTCCTTCTCCTACAGAAATTTCATCCTTCTCCGTAATTTTGCAAATCAAACCTGTAACTTTTCCTTCTCTGTCAACCATTTCTCCCACTCATGGACCTCCGACACCTCTCCCCGATTTCCTCTTCCTCCTCCAGATTTCATCCGAGAGACTCGTCGGGGTTTTGCTAAAGGCCGAAAATCAAATGAATCTTCTACAAGTATGGCTGAGGCGTTGCCTGATATTCGACCCACGATTAAAGCAAACGCTAGCTCGCAAATGGAGGCATCGATAGTTGCATTATCAGGAGAATTAAGCAAGCTTCGAACGGGCAGGGCATCACCTGGAATGCTTGACCACATTATAGTAGAAACTTCAGGTGTGAAGCTGCCGTTGAATCAGATTGCGGCTGTATCGGTCTTGGATTCTAAAACATTGTCAATAAATCCTTATGATCCCAGTACACTAAAGAATTTAGAGACGGCTATAATATCATCTCCTTTAGGTGTAGCACCAAGAGTAGACGGAGAGAGGTTAATTGCAGTCATTCCACCTTTGACCAAAGAACATATCCAGGCTATGTGTAAGCTGGTCACCAAGTGTTGCGAAGATTCCAGACAAAGCATACGACGAGCTCGTCAAAAGGCCATGGATACAGTTAAAAAGATTAGCTCAAGCTACCCAAAGGATGACATAAAAAGACTGGAAAAGGAAGTTGATGAATTGACTAAGAAGTTTATCAAGTCAGCCGAAGAACTCTGCAAGGGAAAGGAGAAGGAGATCACTGGAGGTTAA

Protein sequence

MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRGFAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHIIVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIAVIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDELTKKFIKSAEELCKGKEKEITGG*
Homology
BLAST of Chy3G051030 vs. ExPASy Swiss-Prot
Match: Q5WFT1 (Ribosome-recycling factor OS=Bacillus clausii (strain KSM-K16) OX=66692 GN=frr PE=3 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.7e-28
Identity = 69/178 (38.76%), Postives = 116/178 (65.17%), Query Frame = 0

Query: 85  IKANASSQMEASIVALSGELSKLRTGRASPGMLDHIIVETSGVKLPLNQIAAVSVLDSKT 144
           IKA+A ++M+ +I  LS EL+KLR GRA+P +LD + VE  G + PLNQ+A+V+V +++ 
Sbjct: 5   IKADAKTRMDKAIDVLSRELAKLRAGRANPALLDRVTVEYYGAQTPLNQLASVTVPEARL 64

Query: 145 LSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIAVIPPLTKEHIQAMCKLVTKCCEDS 204
           L I PYD + + ++E AI+ S LG+ P  DG+ +   IPPLT+E  + + +LV+K  E+S
Sbjct: 65  LLITPYDKTAVASIEKAILKSDLGLTPSNDGQVIRIAIPPLTEERRKDLVRLVSKTAEES 124

Query: 205 RQSIRRARQKAMDTVKKI--SSSYPKDDIKRLEKEVDELTKKFIKSAEELCKGKEKEI 261
           + ++R  R+ A D +KK+       +D+++    ++ +LT  ++   +E  K KE+EI
Sbjct: 125 KVAVRNIRRDANDELKKLQKDGEMTEDELRSATDDIQKLTDTYVAKIDEKAKQKEQEI 182

BLAST of Chy3G051030 vs. ExPASy Swiss-Prot
Match: A8MHH2 (Ribosome-recycling factor OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688 GN=frr PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.8e-28
Identity = 71/178 (39.89%), Postives = 110/178 (61.80%), Query Frame = 0

Query: 85  IKANASSQMEASIVALSGELSKLRTGRASPGMLDHIIVETSGVKLPLNQIAAVSVLDSKT 144
           I  N   +M  +I AL  EL+ +R GRA+P MLD I+VE  G   P+ QIAA++  + + 
Sbjct: 5   IHKNLEEKMNKTINALKDELASIRAGRANPAMLDRIVVEYYGSPTPIKQIAAITAPEPRI 64

Query: 145 LSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIAVIPPLTKEHIQAMCKLVTKCCEDS 204
           +SI PYD S L ++E AI+ S LGV P  DG+ +  ++P LT+E  + + K+  K  ED+
Sbjct: 65  ISIQPYDGSALSSIEKAILQSDLGVNPSNDGKLIRIIVPQLTEERRKELTKVAKKTSEDA 124

Query: 205 RQSIRRARQKAMDTVKKI--SSSYPKDDIKRLEKEVDELTKKFIKSAEELCKGKEKEI 261
           +  +R  R+ A D +KK+   +   +D++K  + EV ++T KFIK  +EL + KEK+I
Sbjct: 125 KVVLRNERRNANDRLKKMQKDNEITEDELKTAQDEVQKITDKFIKMVDELTEKKEKDI 182

BLAST of Chy3G051030 vs. ExPASy Swiss-Prot
Match: Q9KA66 (Ribosome-recycling factor OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=frr PE=3 SV=3)

HSP 1 Score: 125.9 bits (315), Expect = 6.6e-28
Identity = 71/175 (40.57%), Postives = 111/175 (63.43%), Query Frame = 0

Query: 88  NASSQMEASIVALSGELSKLRTGRASPGMLDHIIVETSGVKLPLNQIAAVSVLDSKTLSI 147
           +A  +M  +  AL  EL+KLR GRA+P MLD I VE  G + PLNQ+A +SV +++ L I
Sbjct: 8   DAEQRMTKATEALGRELAKLRAGRANPAMLDRITVEYYGAETPLNQLATISVPEARLLVI 67

Query: 148 NPYDPSTLKNLETAIISSPLGVAPRVDGERLIAVIPPLTKEHIQAMCKLVTKCCEDSRQS 207
            P+D S++ ++E AI  S LG+ P  DG  +   IPPLT+E  + + KLV K  E+++ +
Sbjct: 68  QPFDKSSISDIERAIQKSDLGLTPSNDGTVIRITIPPLTEERRRDLTKLVKKSAEEAKVA 127

Query: 208 IRRARQKAMDTVKK--ISSSYPKDDIKRLEKEVDELTKKFIKSAEELCKGKEKEI 261
           +R  R+ A D +KK        +DD++R+ ++V +LT K+I+  ++  + KEKEI
Sbjct: 128 VRNIRRDANDDLKKRQKDGELTEDDLRRVTEDVQKLTDKYIEQIDQKAEAKEKEI 182

BLAST of Chy3G051030 vs. ExPASy Swiss-Prot
Match: A4IMC6 (Ribosome-recycling factor OS=Geobacillus thermodenitrificans (strain NG80-2) OX=420246 GN=frr PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 4.3e-27
Identity = 68/174 (39.08%), Postives = 110/174 (63.22%), Query Frame = 0

Query: 89  ASSQMEASIVALSGELSKLRTGRASPGMLDHIIVETSGVKLPLNQIAAVSVLDSKTLSIN 148
           A  +M+ ++ A S EL+ +R GRA+ G+L+ + V+  GV  P+NQ+A +SV +++ L I 
Sbjct: 9   AKEKMDKAVQAFSRELATVRAGRANAGLLEKVTVDYYGVATPINQLATISVPEARMLVIQ 68

Query: 149 PYDPSTLKNLETAIISSPLGVAPRVDGERLIAVIPPLTKEHIQAMCKLVTKCCEDSRQSI 208
           PYD S +K +E AI++S LGV P  DG  +  VIPPLT+E  + + KLV K  E+++ ++
Sbjct: 69  PYDKSVIKEMEKAILASDLGVTPSNDGSVIRLVIPPLTEERRRELAKLVKKYSEEAKVAV 128

Query: 209 RRARQKAMDTVKKI--SSSYPKDDIKRLEKEVDELTKKFIKSAEELCKGKEKEI 261
           R  R+ A D +KK+  +S   +D+++    EV +LT   I   + + K KEKE+
Sbjct: 129 RNIRRDANDELKKLEKNSEITEDELRSYTDEVQKLTDSHIAKIDAITKEKEKEV 182

BLAST of Chy3G051030 vs. ExPASy Swiss-Prot
Match: Q895K9 (Ribosome-recycling factor OS=Clostridium tetani (strain Massachusetts / E88) OX=212717 GN=frr PE=3 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 5.6e-27
Identity = 69/176 (39.20%), Postives = 109/176 (61.93%), Query Frame = 0

Query: 85  IKANASSQMEASIVALSGELSKLRTGRASPGMLDHIIVETSGVKLPLNQIAAVSVLDSKT 144
           I   A  +M  +++AL  +L+ L+ GRA+P MLD I  E  G   PL+Q+A +SV +++ 
Sbjct: 5   IMNTAEDKMSKALLALKKDLASLKAGRANPAMLDKIEAEYYGTMTPLSQLANISVPEARI 64

Query: 145 LSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIAVIPPLTKEHIQAMCKLVTKCCEDS 204
           L I P+D S+LK +E AI+ S LG+ P  DG  +  +IP LT+E  + + K V K  ED+
Sbjct: 65  LQIQPWDKSSLKAIEKAILVSDLGLNPNNDGTIIRLIIPELTEETRRNIVKTVKKYGEDT 124

Query: 205 RQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDELTKKFIKSAEELCKGKEKEI 261
           + +IR  R+   D +K + S   +DDIK+ E+E+ ++T  ++K  +E+   KEKEI
Sbjct: 125 KIAIRSVRRDGNDKIKDLKSDMSEDDIKKAEEEIQKITDNYVKKVDEMIDIKEKEI 180

BLAST of Chy3G051030 vs. ExPASy TrEMBL
Match: A0A0A0KA50 (Ribosome-recycling factor, chloroplastic OS=Cucumis sativus OX=3659 GN=Csa_6G008020 PE=3 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 5.6e-139
Identity = 263/263 (100.00%), Postives = 263/263 (100.00%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG
Sbjct: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI
Sbjct: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA
Sbjct: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 264
           LTKKFIKSAEELCKGKEKEITGG
Sbjct: 241 LTKKFIKSAEELCKGKEKEITGG 263

BLAST of Chy3G051030 vs. ExPASy TrEMBL
Match: A0A5D3DLY3 (Ribosome-recycling factor, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold205G00450 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 5.2e-129
Identity = 247/263 (93.92%), Postives = 256/263 (97.34%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MA+FLKRSFSYRNFI+LRNFANQTCNFSFS+NHFS+SWTSDTS  F LPP DFIRE+RRG
Sbjct: 1   MAMFLKRSFSYRNFIILRNFANQTCNFSFSLNHFSNSWTSDTSAGFALPPSDFIRESRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTS+AEAL DIRPTIK+NASSQMEASIVALSGELSKLRTGRAS GMLDHI
Sbjct: 61  FAKGRKSNESSTSIAEALTDIRPTIKSNASSQMEASIVALSGELSKLRTGRASSGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLE+AIISSPLGVAPRVDGERLIA
Sbjct: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLESAIISSPLGVAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDD+KRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDMKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 264
           LTKKFIKSAEELCK KEKEITGG
Sbjct: 241 LTKKFIKSAEELCKAKEKEITGG 263

BLAST of Chy3G051030 vs. ExPASy TrEMBL
Match: A0A1S3B2S9 (Ribosome-recycling factor, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485499 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 5.2e-129
Identity = 247/263 (93.92%), Postives = 256/263 (97.34%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MA+FLKRSFSYRNFI+LRNFANQTCNFSFS+NHFS+SWTSDTS  F LPP DFIRE+RRG
Sbjct: 1   MAMFLKRSFSYRNFIILRNFANQTCNFSFSLNHFSNSWTSDTSAGFALPPSDFIRESRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTS+AEAL DIRPTIK+NASSQMEASIVALSGELSKLRTGRAS GMLDHI
Sbjct: 61  FAKGRKSNESSTSIAEALTDIRPTIKSNASSQMEASIVALSGELSKLRTGRASSGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLE+AIISSPLGVAPRVDGERLIA
Sbjct: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLESAIISSPLGVAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDD+KRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDMKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 264
           LTKKFIKSAEELCK KEKEITGG
Sbjct: 241 LTKKFIKSAEELCKAKEKEITGG 263

BLAST of Chy3G051030 vs. ExPASy TrEMBL
Match: A0A6J1GLU1 (Ribosome-recycling factor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455524 PE=3 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 1.9e-118
Identity = 229/263 (87.07%), Postives = 245/263 (93.16%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MA+FLKRSFSYRNF+LLRNFAN + NFSF V    + WTS+    F LPP DFIRE+RRG
Sbjct: 1   MAMFLKRSFSYRNFLLLRNFANPSRNFSFFVYDLCNPWTSEMPGAFALPPADFIRESRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEA+I ALSGELSKLRTGRAS GMLDHI
Sbjct: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEAAISALSGELSKLRTGRASSGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVE SGV++PLNQIAAVSVLDSKTLSINPYDP+TLKNLE+AI+SSPLG+APRVDGERLIA
Sbjct: 121 IVEISGVRMPLNQIAAVSVLDSKTLSINPYDPNTLKNLESAIVSSPLGLAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKK+SSSYPKDD+KRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKMSSSYPKDDMKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 264
           LTKKFIKSAE+LCK KEKEITGG
Sbjct: 241 LTKKFIKSAEDLCKAKEKEITGG 263

BLAST of Chy3G051030 vs. ExPASy TrEMBL
Match: A0A6J1JKE4 (Ribosome-recycling factor, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487708 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 4.2e-118
Identity = 228/263 (86.69%), Postives = 245/263 (93.16%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MA+FLKRSF YR+F+LLRNFAN + NFSF VN   + WTS+    F LPP DFIRE+RRG
Sbjct: 1   MAMFLKRSFFYRSFLLLRNFANPSRNFSFFVNDLCNPWTSEMPGVFALPPADFIRESRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEA+I ALSGELSKLRTGRAS GMLDHI
Sbjct: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEAAIAALSGELSKLRTGRASSGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVE SGV++PLNQIAAVSVLDSKTLSINPYDP+TLKNLE+AI+SSPLG+APRVDGERLIA
Sbjct: 121 IVEISGVRMPLNQIAAVSVLDSKTLSINPYDPNTLKNLESAIVSSPLGLAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKK+SSSYPKDD+KRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKMSSSYPKDDMKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 264
           LTKKFIKSAE+LCK KEKEITGG
Sbjct: 241 LTKKFIKSAEDLCKAKEKEITGG 263

BLAST of Chy3G051030 vs. NCBI nr
Match: XP_004138478.2 (uncharacterized protein LOC101216947 [Cucumis sativus] >KGN45719.1 hypothetical protein Csa_004901 [Cucumis sativus])

HSP 1 Score: 508 bits (1308), Expect = 3.49e-181
Identity = 263/263 (100.00%), Postives = 263/263 (100.00%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG
Sbjct: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI
Sbjct: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA
Sbjct: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 263
           LTKKFIKSAEELCKGKEKEITGG
Sbjct: 241 LTKKFIKSAEELCKGKEKEITGG 263

BLAST of Chy3G051030 vs. NCBI nr
Match: XP_008441363.1 (PREDICTED: ribosome-recycling factor [Cucumis melo])

HSP 1 Score: 475 bits (1222), Expect = 4.49e-168
Identity = 247/263 (93.92%), Postives = 256/263 (97.34%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MA+FLKRSFSYRNFI+LRNFANQTCNFSFS+NHFS+SWTSDTS  F LPP DFIRE+RRG
Sbjct: 1   MAMFLKRSFSYRNFIILRNFANQTCNFSFSLNHFSNSWTSDTSAGFALPPSDFIRESRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTS+AEAL DIRPTIK+NASSQMEASIVALSGELSKLRTGRAS GMLDHI
Sbjct: 61  FAKGRKSNESSTSIAEALTDIRPTIKSNASSQMEASIVALSGELSKLRTGRASSGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLE+AIISSPLGVAPRVDGERLIA
Sbjct: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLESAIISSPLGVAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDD+KRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDMKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 263
           LTKKFIKSAEELCK KEKEITGG
Sbjct: 241 LTKKFIKSAEELCKAKEKEITGG 263

BLAST of Chy3G051030 vs. NCBI nr
Match: KAA0056597.1 (ribosome-recycling factor [Cucumis melo var. makuwa] >TYK24280.1 ribosome-recycling factor [Cucumis melo var. makuwa])

HSP 1 Score: 475 bits (1222), Expect = 1.03e-167
Identity = 247/263 (93.92%), Postives = 256/263 (97.34%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MA+FLKRSFSYRNFI+LRNFANQTCNFSFS+NHFS+SWTSDTS  F LPP DFIRE+RRG
Sbjct: 1   MAMFLKRSFSYRNFIILRNFANQTCNFSFSLNHFSNSWTSDTSAGFALPPSDFIRESRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTS+AEAL DIRPTIK+NASSQMEASIVALSGELSKLRTGRAS GMLDHI
Sbjct: 61  FAKGRKSNESSTSIAEALTDIRPTIKSNASSQMEASIVALSGELSKLRTGRASSGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLE+AIISSPLGVAPRVDGERLIA
Sbjct: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLESAIISSPLGVAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDD+KRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDMKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 263
           LTKKFIKSAEELCK KEKEITGG
Sbjct: 241 LTKKFIKSAEELCKAKEKEITGG 263

BLAST of Chy3G051030 vs. NCBI nr
Match: KAG7033363.1 (frr [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 443 bits (1139), Expect = 1.99e-155
Identity = 230/263 (87.45%), Postives = 246/263 (93.54%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MA+FLKRSFSYRNF+LLRNFAN + NFSF VN   + WTS+    F LPP DFIRE+RRG
Sbjct: 1   MAMFLKRSFSYRNFLLLRNFANPSRNFSFFVNDLCNPWTSEMPGAFALPPADFIRESRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEA+I ALSGELSKLRTGRAS GMLDHI
Sbjct: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEAAISALSGELSKLRTGRASSGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVE SGV++PLNQIAAVSVLDSKTLSINPYDP+TLKNLE+AI+SSPLG+APRVDGERLIA
Sbjct: 121 IVEISGVRMPLNQIAAVSVLDSKTLSINPYDPNTLKNLESAIVSSPLGLAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKK+SSSYPKDD+KRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKMSSSYPKDDMKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 263
           LTKKFIKSAE+LCK KEKEITGG
Sbjct: 241 LTKKFIKSAEDLCKAKEKEITGG 263

BLAST of Chy3G051030 vs. NCBI nr
Match: XP_023544000.1 (uncharacterized protein LOC111803704 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 442 bits (1138), Expect = 2.83e-155
Identity = 230/263 (87.45%), Postives = 246/263 (93.54%), Query Frame = 0

Query: 1   MAIFLKRSFSYRNFILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRETRRG 60
           MA+FLKRSFSYRNF+LLRNFAN + N SF VN   +SWTS+    F LPP DFIRE+RRG
Sbjct: 1   MAMFLKRSFSYRNFLLLRNFANPSRNLSFFVNDLCNSWTSEMPGAFALPPADFIRESRRG 60

Query: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHI 120
           FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEA+I ALSGELSKLRTGRAS GMLDHI
Sbjct: 61  FAKGRKSNESSTSMAEALPDIRPTIKANASSQMEAAISALSGELSKLRTGRASSGMLDHI 120

Query: 121 IVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIA 180
           IVE SGV++PLNQIAAVSVLDSKTLSINPYDP+TLKNLE+AI+SSPLG+APRVDGERLIA
Sbjct: 121 IVEISGVRMPLNQIAAVSVLDSKTLSINPYDPNTLKNLESAIVSSPLGLAPRVDGERLIA 180

Query: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEKEVDE 240
           VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKK+SSSYPKDD+KRLEKEVDE
Sbjct: 181 VIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKMSSSYPKDDMKRLEKEVDE 240

Query: 241 LTKKFIKSAEELCKGKEKEITGG 263
           LTKKFIKSAE+LCK KEKEITGG
Sbjct: 241 LTKKFIKSAEDLCKAKEKEITGG 263

BLAST of Chy3G051030 vs. TAIR 10
Match: AT3G01800.1 (Ribosome recycling factor )

HSP 1 Score: 306.6 bits (784), Expect = 1.9e-83
Identity = 166/265 (62.64%), Postives = 211/265 (79.62%), Query Frame = 0

Query: 1   MAIFLKRSFSYRN---FILLRNFANQTCNFSFSVNHFSHSWTSDTSPRFPLPPPDFIRET 60
           MA+F++R+ S RN   F+   +   Q+ +FSF ++  +         +F L  PDF+R++
Sbjct: 2   MAMFVRRALSSRNAIGFLRRSSVVVQSRDFSF-ISSPNLMMMGGRDLQFDLSMPDFLRDS 61

Query: 61  RRGFAKGRKS-NESSTSMAEALPDIRPTIKANASSQMEASIVALSGELSKLRTGRASPGM 120
           RRGFAKG+KS ++S T M +A PDI PT+KA ASSQMEA+I ALS +L+KLRTGRA+PGM
Sbjct: 62  RRGFAKGKKSKDDSGTGMVDAAPDIGPTVKAAASSQMEAAIDALSRDLTKLRTGRAAPGM 121

Query: 121 LDHIIVETSGVKLPLNQIAAVSVLDSKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGE 180
           LDHI+VET GVK+PLN +A VSVLD KTLS+NPYDP T+K LE AI++SPLG+ P++DG+
Sbjct: 122 LDHIVVETGGVKMPLNHLALVSVLDPKTLSVNPYDPDTVKELEKAIVASPLGLNPKLDGQ 181

Query: 181 RLIAVIPPLTKEHIQAMCKLVTKCCEDSRQSIRRARQKAMDTVKKISSSYPKDDIKRLEK 240
           RL+A IP LTKEHIQAMCK+VTK  E  +QSIRRARQKA+DT+KK  SS PKD++KRLEK
Sbjct: 182 RLVASIPALTKEHIQAMCKIVTKSSEVVKQSIRRARQKALDTIKKAGSSLPKDEVKRLEK 241

Query: 241 EVDELTKKFIKSAEELCKGKEKEIT 262
           EVDELTKKF+KSAE++CK KEKEIT
Sbjct: 242 EVDELTKKFVKSAEDMCKSKEKEIT 265

BLAST of Chy3G051030 vs. TAIR 10
Match: AT3G63190.1 (ribosome recycling factor, chloroplast precursor )

HSP 1 Score: 109.0 bits (271), Expect = 5.9e-24
Identity = 64/181 (35.36%), Postives = 105/181 (58.01%), Query Frame = 0

Query: 82  RPTIKANASSQMEASIVALSGELSKLRTGRASPGMLDHIIVETSGVKLPLNQIAAVSVLD 141
           +  I+ +  S+ME +I  L    + +RTGR++  MLD I VE  G  + L  IA +S  D
Sbjct: 92  KSAIETDVKSKMEKTIETLRTSFNSIRTGRSNAAMLDKIEVEYYGSPVSLKSIAQISTPD 151

Query: 142 SKTLSINPYDPSTLKNLETAIISSPLGVAPRVDGERLIAVIPPLTKEHIQAMCKLVTKCC 201
             +L + PYD S+LK +E AI++S LGV P  DG+ +   +PPLT +  + + K+V K  
Sbjct: 152 GSSLLLQPYDKSSLKAIEKAIVNSDLGVTPNNDGDVIRLSLPPLTSDRRKELSKVVAKQS 211

Query: 202 EDSRQSIRRARQKAMDTVKKI--SSSYPKDDIKRLEKEVDELTKKFIKSAEELCKGKEKE 261
           E+ + ++R  R+ A+ +  K+       +D++K L  ++ +L   ++K  EEL K KEKE
Sbjct: 212 EEGKVALRNIRRDALKSYDKLEKEKKLSEDNVKDLSSDLQKLIDVYMKKIEELYKQKEKE 271

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5WFT11.7e-2838.76Ribosome-recycling factor OS=Bacillus clausii (strain KSM-K16) OX=66692 GN=frr P... [more]
A8MHH23.8e-2839.89Ribosome-recycling factor OS=Alkaliphilus oremlandii (strain OhILAs) OX=350688 G... [more]
Q9KA666.6e-2840.57Ribosome-recycling factor OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 1819... [more]
A4IMC64.3e-2739.08Ribosome-recycling factor OS=Geobacillus thermodenitrificans (strain NG80-2) OX=... [more]
Q895K95.6e-2739.20Ribosome-recycling factor OS=Clostridium tetani (strain Massachusetts / E88) OX=... [more]
Match NameE-valueIdentityDescription
A0A0A0KA505.6e-139100.00Ribosome-recycling factor, chloroplastic OS=Cucumis sativus OX=3659 GN=Csa_6G008... [more]
A0A5D3DLY35.2e-12993.92Ribosome-recycling factor, chloroplastic OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A1S3B2S95.2e-12993.92Ribosome-recycling factor, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485499... [more]
A0A6J1GLU11.9e-11887.07Ribosome-recycling factor, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1JKE44.2e-11886.69Ribosome-recycling factor, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
Match NameE-valueIdentityDescription
XP_004138478.23.49e-181100.00uncharacterized protein LOC101216947 [Cucumis sativus] >KGN45719.1 hypothetical ... [more]
XP_008441363.14.49e-16893.92PREDICTED: ribosome-recycling factor [Cucumis melo][more]
KAA0056597.11.03e-16793.92ribosome-recycling factor [Cucumis melo var. makuwa] >TYK24280.1 ribosome-recycl... [more]
KAG7033363.11.99e-15587.45frr [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023544000.12.83e-15587.45uncharacterized protein LOC111803704 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT3G01800.11.9e-8362.64Ribosome recycling factor [more]
AT3G63190.15.9e-2435.36ribosome recycling factor, chloroplast precursor [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 231..251
NoneNo IPR availableGENE3D3.30.1360.40coord: 111..185
e-value: 2.6E-55
score: 188.6
NoneNo IPR availablePANTHERPTHR20982:SF12OSJNBA0076N16.8 PROTEINcoord: 1..262
IPR036191RRF superfamilyGENE3D1.10.132.20coord: 90..260
e-value: 2.6E-55
score: 188.6
IPR036191RRF superfamilySUPERFAMILY55194Ribosome recycling factor, RRFcoord: 85..260
IPR023584Ribosome recycling factor domainPFAMPF01765RRFcoord: 101..260
e-value: 2.4E-55
score: 186.9
IPR002661Ribosome recycling factorPANTHERPTHR20982RIBOSOME RECYCLING FACTORcoord: 1..262
IPR002661Ribosome recycling factorCDDcd00520RRFcoord: 85..260
e-value: 1.64936E-51
score: 164.355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G051030.1Chy3G051030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0043023 ribosomal large subunit binding