Chy3G050280 (gene) Cucumber (hystrix) v1

Overview
NameChy3G050280
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Description53EXOc domain-containing protein
LocationchrH03: 540225 .. 545692 (-)
RNA-Seq ExpressionChy3G050280
SyntenyChy3G050280
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGAGGCGAGCGCCAACATTGGCGTTAATTTTCCGCCATTTTTGAACTCTTCATCTCCTACTTTCTTACCCTCAAGAACTCTGAAACCCGAATCCCCACTAACATCCAAACTCAAACCCAATACATGGAGAACGAAGCCGCTGAATCTAACTGCCTTTGCACCATTTTCTCGTCTTACTTCTGCGGCTTTTAATCAAACGGATGATGGGAAGTTTCAGCCAAGAATTGAAGCTGATAATTCAAGAACGAGAAGGGTTTTTTTCCTGGATGTAAATCCTTTATGCTATCAAGGTAACAAACCTAGTTTGCGCAATTTTGGTCGCTGGGTTTCCATCTTCTTCGAGGAAGTTAGCCACAGTGATCCTGTTATTGCTGTGAGTACCATCGTCTCTGTATTGCATTTATTATCAGCAATGGCGAGCGATTAGTTTGTTTGATTTTGTACACTTTATATATCTATATACACATGCTACTGAGTTGGCGCCTAATTTTTATACATTCTTGTGGATTTCGGTTCCAATGTCTGTGTTCAGTCCATTATGATGGCTAGGTTTGATATTCTATGAATTCTCGTGGCAACTGATTTAACTTATTTAACCATTAACCATCAATGAAAATTCTATGGCTCCTCTATAGCCAAATGGGACACAAAGGTGCTACAGATCAGCTCTCCTAAGAGTTAAGAGTTTTAGCCCTGCTTTTGAGCTCCCATCTCACGAAAAACTCTTGTATCCAATCGATAGTCATTTGGGGAAACAACAGATCAGCTGAGACTAAATGCTTTCAGAAGTGACGGACATCTGCATAATCTCTTGATGATGAAAACACGAGCATGTTTCTTTTTATTTTTTAACATGGACCTTTTCACTTTGTTTATGTATGGTTGTACTGTTGTCGAGCAGACATGTTAGAGGACATTGCATGACATAAGGTCTCGTATTTTGTCCACAATTTGTAGGTTGAAGGTTGTTCTTTGTTCAAATGGTGTTTTCTATTTTATGTTTCTAGATCATTTCCAAATTTGGAATGTTGAGTGCCCGTGTGTTCTATTTGCTTCTTCAATCAACTGTTTGTGGTGTGGGAATTACAAGATCAACTTTTAACCGAGAATAAAATATGACTCCCTGCCTGCCCTCCAGCTGAATAGTGTTTCTAGTACTGAAAGTTGGGTAAAGTTTTGGATCTCATTTTCTTTAATAGCATTTTGGGACCAATGTTTAGTGCATCGGTTAAATGTTAGGAACTCATCGTCAAAAGTTTTGAGGAGAGATTTTCATTTCTCCTTAAGTGTACGTGCAAGTATATTGAGTCTGGCATTATTTTGTGGTCTTTTGTTGGAGTAAGTCAAGTTCATCACTTGGAAGATGGATGTACACAACCATGTAATGCTGCAATATGATTGCTTTTCAATTTTTCTTTGTGCATCAAATTTCTCCCAACTCTTGTTACGCTGTCCCTATAGCCTTGTAACTTAGATTTTGTCATTTTCTTATTTGCATTGTCGGATAGCTAGATATTTTACTTCGACTGAGGCTTGTTTTCTTTGACTAAATTTCTCTTAATAAAGAAAATGTTATCAGAATCAGTAGTATGCATTATTTCTCTAACTCTTCAAATTTGTTTTGCTTCTGCAGGTTTTTGATGGGGAAGGAGGTAGTGAGCATCGCAGGCTGTTGTTACCCTCATATAAAGCACATCGGATCAAATTCACGAGACCACCATCTTCAAAAAGATTTACAAAGGGAAATTTTAGAACGTCATACCAAGTGATAAGAGATGCTCTAAGAAGCTGTAATGTTCCAGTATGTGATTTTTTGTCTGAGTCTAAATAATTTGTGACATATTTGGTTGAACTTATTGATTGTGGTGTGGACTAGCTGCTGTTAATTGCAGCAAGCTGACCTCTCTATTCTTTAGGACAAATTTGTTGCTGTCTGAGTATTAATTTCTTAAAGGACACGTTAAGGGTCTTCTTTTCATGCCAAGAAACTACATCTTGCAGGTTGTAAGGGTTGAGGGTCACGAAGCAGATGATGTTATTGCTACACTTGTGGAACAAGTTTTGCAAAGAGGGGTCCGGGTGGTAGTAGCCTCTCCTGATAAAGATTTCAAGCAGTTGATTTCAGAAGATGTCCAACTTGTGATGCCTTTGCCGGAGCTCAACAGATGGTCCTTTTACACCTTAAGGCACTACCTAGCTCAGTACAACTGTGACCCGTGCTCCGACTTGAGTCTTAGTAAGTACTCTGCTACAAATATAGAATGTAGGAACCATTTATAAAGAAAAATTCAGACACACATACCATTCTTTTAAAATCTAAAAAACGATCCAGCCCTTTCGAATATATGCATGTGTTGACAAAAAATAAAACTAATAATAAATGTTGAAGTATGGTATCATGATTTGTTGCATAGGATGCATTATGGGTGATGAGGTAGATGGCGTTCCAGGAATCCAACATGTTGCCCCAGGATTTGGTCGAAAAACAGCATTGAAGCTCTTGAAGAAACATGGTTCCTTGGAGAATCTACTCAGTGCTGCTGCAATCAGAACTGTGGGCAAACCTTACGCACAAGATGCACTTACAAAGTATGCCGAATACCTGCGAACAAACTATAGAGTTCTAGCCTTAAGGAGGTTCTTCCTTTCCTTTCAATTTCACCTTACGCATGTCCTTGAATTCTAGCTGATTTCTTGACTGGGTTTTTATGGCTTTTGGTCGAATTGTTGTACAGAGATGTTGATGTTCAATTTCAAGATGAGTGGTTAGTTGAAAGAGATAGACGAAACGATTCGACTATTTTATCCAAGTTTGTAGAAAACAATGACAGAAACTTGCTCGTTCAACCATCTAAACAGGTCTAAATCCCACTATGGGTGAATACATACAGGTAAAAAACTAGCTTAGCCTTTGTTAGATTATGATTTTATGATTGGAAAATACACACCTCCATACTATTAGTTGCTGAGATTAAAAAAAAAATGTATTATTTATTTTCTTCTCATATGTTGTGGAATGAACTAATCTATATGCAATATCTCAGGGATCTATTAGTACACTGTTTTTACTCTAAACAAATGTCTGTCATTCATTTGTTCAGACAGGTAACTAACATCATCAGGTGGGTTAAAAAATATTCTAATCTAATTTTGCCTCTACTTTACCTCCTTCCAATTCCAGCTGAGTTTAGACAAAAACAAGAAAAATTGTTTCAGTGATTGCATTGTAGTCACTCAGAAAAGGGAAAGAAACAGACGTGGAAGGACGGAACAAGCATTGAGATGTGTGCTGCTTGTCTTGATTTGGGTAAACAAAACAATAGTTGTGGTAAAACTAAAAGTAGTAGGAAAGGCAGAATGTTCTGAATTGTATTCAGATTCTTAGGGAATAACCTATTAGAAACAGACAGACAGACCCAAATAGCAAAAGCTGTTTGTTGATTTTTGCCACTTATTAAAAAAAAACCATATCATCTTACTTATCCACATTCTTGGGTCTTTCCCTATCAATCATAGTCAAAACACACTATTTCGGATTTGAAAATACAATAAATTTTCTTTCTTTTTATAGTTTACAAATGCATACTCTGTATTTGAGTTACTCTTTGATGAAGCTTTATATTGAATTTGAGGATTGAGTATGAATGTTTTGGTTGGATATCTAGTTTTCTTTCCAGCAAAATATTTATATTCCACTATTTATTAATAAGCTTAGTATTATTATTATTGTTATTATCTTTTAATTTTTAATTTAGAGAAGGTAAGTACAAAATTAGGTGAGGAAAAATAGGCACTTGATGTGTCTAAAAATAGAATGGAGCAATTGGTAATAGCAATCAAGGCAATTGAAACAAACGTGATTGATTAGAGAGTTGGACTAAAATCGAGTTGGACCAATTAAAAGCGATGAAATCTATCCAATAGGAACCAATGAAAATGATGCAACTTAATAATTAACAATTCAAGTAGAGAAAAAAAAACCCAAAATAGAAATTGTACTCTGCCATAAGAACTCATAAAACCTATCTACTTTTTGTTCATTTTGTGTAAGGGTCTTTCGCACAACAAATAAATGTTGAATTTTGAAAGTCATTTTTGTAAAAATTATCTAAATAGCTTTTGAAATAATTTATTGTATTTTTTCTTATCGTTTTTAACCAAATAGTTTGAATAAAATTTGTTCTTTGCGATATAGTCATCAATAAGTTTGACTATATTTACCATTTATACACTTAACAACTCTTTAACATTTTCTTAAGTTAAATGGAGAATTTTTTTCAGGCCAAAGTTAGAAATTTAAATTGTGATTTAAAATAATTTTTGAACAAAAAAGAAAAATTCAAAGCAAATTTTGAAATAATATATATATATTAAAAAAGAATGAAATTGTTGTGTGCTGTTTGTATATGTAAAAGTATTTGAGGAGCGTGAGATGGGCACGCTTAAAACCACACAACCCTCTAAATAATAATCCCTTTCTCTTTCTTTCTTTCTTTCTTTGTGATTCCAATTCAATCCGCCATGGATTTCCTTAACAACTACTGAACCCTTTCTTCTCCACCAAACCTCACTCCCATGGCTCTTCTTCTTCCTCATCTCTTCCCTTCACTCTCCCTTCACTCCAAATCCAAAGACAATTCTCTTCTCTTTAAACCCTCTTCTTCTTCTTCTTCTTCTCCTTCTCCTTCTCTTTCTCTTACCAGTGTTCATTCTCCCCAGCTTCAAGGCTCTCAACTCGCTTCTCCCCCTGGTTCTCCCGATTCCCGTTCTGATAAGCCTAGAGACGACTTCTATGTCAATCTCGGCCTCGCTGTTAGAACTCTTCGTGAAGACCTTCCTCTAATTTTCACCAGAGACCTCAATTACGACATTTACAGGTACTTTTCTTTCTTTCTTTTAACGTACGTATTTCTGTTTTTCTTATTCATTTCTTTCTCTCGTCTCCTACTTATGTTGTTCTGGTGGGGATTTTCCAGGGACGATATAACTTTTACTGATCCTCTCAACACGTTTACTGGTATTGAGAGGTACAAATTGATCTTCTGGGCTCTGAGATTTCATGGCAAAATTCTTTTCCGTGAGATTGGAATTGAGGTTTACAGGATTTGGCAACCTTCTGAAAACGTTATATTGATTCGGTGGAACTTGAAGGGTGTTCCTAGGGTTCCATGGGAAGCTAGGGGTGAATTTCAAGGGACTTCTAGGTATAAAGTGGATCGAAATGGGAAAATTTATGAACACAAAGTTGATAATTTAGCATTTAATTTCCCTCAGCAATTGAAGCCAGCTGCGTCAGTCTTGGATTTGGTTTCTGCTTGTCCTGCAAGCCCTAATCCAACTTTCTTGTGGGGAACAGAGGATTTGCATTGTTCTTCATGGGTTGAGCTTTATCAATCTGTGAGGAGAAGTGTAGGAGGAGAAGGGTATTTGATTACACAAGATGGATTTCTTACATGTTCATAG

mRNA sequence

ATGGCCGAGGCGAGCGCCAACATTGGCGTTAATTTTCCGCCATTTTTGAACTCTTCATCTCCTACTTTCTTACCCTCAAGAACTCTGAAACCCGAATCCCCACTAACATCCAAACTCAAACCCAATACATGGAGAACGAAGCCGCTGAATCTAACTGCCTTTGCACCATTTTCTCGTCTTACTTCTGCGGCTTTTAATCAAACGGATGATGGGAAGTTTCAGCCAAGAATTGAAGCTGATAATTCAAGAACGAGAAGGGTTTTTTTCCTGGATGTAAATCCTTTATGCTATCAAGGTAACAAACCTAGTTTGCGCAATTTTGGTCGCTGGGTTTCCATCTTCTTCGAGGAAGTTAGCCACAGTGATCCTGTTATTGCTGTTTTTGATGGGGAAGGAGGTAGTGAGCATCGCAGGCTGTTGTTACCCTCATATAAAGCACATCGGATCAAATTCACGAGACCACCATCTTCAAAAAGATTTACAAAGGGAAATTTTAGAACGTCATACCAAGTGATAAGAGATGCTCTAAGAAGCTGTAATGTTCCAGTTGTAAGGGTTGAGGGTCACGAAGCAGATGATGTTATTGCTACACTTGTGGAACAAGTTTTGCAAAGAGGGGTCCGGGTGGTAGTAGCCTCTCCTGATAAAGATTTCAAGCAGTTGATTTCAGAAGATGTCCAACTTGTGATGCCTTTGCCGGAGCTCAACAGATGGTCCTTTTACACCTTAAGGCACTACCTAGCTCAGTACAACTGTGACCCGTGCTCCGACTTGAGTCTTAGATGCATTATGGGTGATGAGGTAGATGGCGTTCCAGGAATCCAACATGTTGCCCCAGGATTTGGTCGAAAAACAGCATTGAAGCTCTTGAAGAAACATGGTTCCTTGGAGAATCTACTCAGTGCTGCTGCAATCAGAACTGTGGGCAAACCTTACGCACAAGATGCACTTACAAAGTATGCCGAATACCTGCGAACAAACTATAGAGTTCTAGCCTTAAGGAGAGATGTTGATGTTCAATTTCAAGATGAGTGGTTAGTTGAAAGAGATAGACGAAACGATTCGACTATTTTATCCAAGTTTGTAGAAAACAATGACAGAAACTTGCTCGTTCAACCATCTAAACAGCTTCAAGGCTCTCAACTCGCTTCTCCCCCTGGTTCTCCCGATTCCCGTTCTGATAAGCCTAGAGACGACTTCTATGTCAATCTCGGCCTCGCTGTTAGAACTCTTCGTGAAGACCTTCCTCTAATTTTCACCAGAGACCTCAATTACGACATTTACAGGTACTTTTCTTTCTTTCTTTTAACGTACGTATTTCTGTTTTTCTTATTCATTTCTTTCTCTCGTCTCCTACTTATGTTGTTCTGGTGGGGATTTTCCAGGGACGATATAACTTTTACTGATCCTCTCAACACGTTTACTGGTATTGAGAGGTACAAATTGATCTTCTGGGCTCTGAGATTTCATGGCAAAATTCTTTTCCGTGAGATTGGAATTGAGGTTTACAGGATTTGGCAACCTTCTGAAAACGTTATATTGATTCGGTGGAACTTGAAGGGTGTTCCTAGGGTTCCATGGGAAGCTAGGGGTGAATTTCAAGGGACTTCTAGGTATAAAGTGGATCGAAATGGGAAAATTTATGAACACAAAGTTGATAATTTAGCATTTAATTTCCCTCAGCAATTGAAGCCAGCTGCGTCAGTCTTGGATTTGGTTTCTGCTTGTCCTGCAAGCCCTAATCCAACTTTCTTGTGGGGAACAGAGGATTTGCATTGTTCTTCATGGGTTGAGCTTTATCAATCTGTGAGGAGAAGTGTAGGAGGAGAAGGGTATTTGATTACACAAGATGGATTTCTTACATGTTCATAG

Coding sequence (CDS)

ATGGCCGAGGCGAGCGCCAACATTGGCGTTAATTTTCCGCCATTTTTGAACTCTTCATCTCCTACTTTCTTACCCTCAAGAACTCTGAAACCCGAATCCCCACTAACATCCAAACTCAAACCCAATACATGGAGAACGAAGCCGCTGAATCTAACTGCCTTTGCACCATTTTCTCGTCTTACTTCTGCGGCTTTTAATCAAACGGATGATGGGAAGTTTCAGCCAAGAATTGAAGCTGATAATTCAAGAACGAGAAGGGTTTTTTTCCTGGATGTAAATCCTTTATGCTATCAAGGTAACAAACCTAGTTTGCGCAATTTTGGTCGCTGGGTTTCCATCTTCTTCGAGGAAGTTAGCCACAGTGATCCTGTTATTGCTGTTTTTGATGGGGAAGGAGGTAGTGAGCATCGCAGGCTGTTGTTACCCTCATATAAAGCACATCGGATCAAATTCACGAGACCACCATCTTCAAAAAGATTTACAAAGGGAAATTTTAGAACGTCATACCAAGTGATAAGAGATGCTCTAAGAAGCTGTAATGTTCCAGTTGTAAGGGTTGAGGGTCACGAAGCAGATGATGTTATTGCTACACTTGTGGAACAAGTTTTGCAAAGAGGGGTCCGGGTGGTAGTAGCCTCTCCTGATAAAGATTTCAAGCAGTTGATTTCAGAAGATGTCCAACTTGTGATGCCTTTGCCGGAGCTCAACAGATGGTCCTTTTACACCTTAAGGCACTACCTAGCTCAGTACAACTGTGACCCGTGCTCCGACTTGAGTCTTAGATGCATTATGGGTGATGAGGTAGATGGCGTTCCAGGAATCCAACATGTTGCCCCAGGATTTGGTCGAAAAACAGCATTGAAGCTCTTGAAGAAACATGGTTCCTTGGAGAATCTACTCAGTGCTGCTGCAATCAGAACTGTGGGCAAACCTTACGCACAAGATGCACTTACAAAGTATGCCGAATACCTGCGAACAAACTATAGAGTTCTAGCCTTAAGGAGAGATGTTGATGTTCAATTTCAAGATGAGTGGTTAGTTGAAAGAGATAGACGAAACGATTCGACTATTTTATCCAAGTTTGTAGAAAACAATGACAGAAACTTGCTCGTTCAACCATCTAAACAGCTTCAAGGCTCTCAACTCGCTTCTCCCCCTGGTTCTCCCGATTCCCGTTCTGATAAGCCTAGAGACGACTTCTATGTCAATCTCGGCCTCGCTGTTAGAACTCTTCGTGAAGACCTTCCTCTAATTTTCACCAGAGACCTCAATTACGACATTTACAGGTACTTTTCTTTCTTTCTTTTAACGTACGTATTTCTGTTTTTCTTATTCATTTCTTTCTCTCGTCTCCTACTTATGTTGTTCTGGTGGGGATTTTCCAGGGACGATATAACTTTTACTGATCCTCTCAACACGTTTACTGGTATTGAGAGGTACAAATTGATCTTCTGGGCTCTGAGATTTCATGGCAAAATTCTTTTCCGTGAGATTGGAATTGAGGTTTACAGGATTTGGCAACCTTCTGAAAACGTTATATTGATTCGGTGGAACTTGAAGGGTGTTCCTAGGGTTCCATGGGAAGCTAGGGGTGAATTTCAAGGGACTTCTAGGTATAAAGTGGATCGAAATGGGAAAATTTATGAACACAAAGTTGATAATTTAGCATTTAATTTCCCTCAGCAATTGAAGCCAGCTGCGTCAGTCTTGGATTTGGTTTCTGCTTGTCCTGCAAGCCCTAATCCAACTTTCTTGTGGGGAACAGAGGATTTGCATTGTTCTTCATGGGTTGAGCTTTATCAATCTGTGAGGAGAAGTGTAGGAGGAGAAGGGTATTTGATTACACAAGATGGATTTCTTACATGTTCATAG

Protein sequence

MAEASANIGVNFPPFLNSSSPTFLPSRTLKPESPLTSKLKPNTWRTKPLNLTAFAPFSRLTSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSHSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCNVPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLLSAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILSKFVENNDRNLLVQPSKQLQGSQLASPPGSPDSRSDKPRDDFYVNLGLAVRTLREDLPLIFTRDLNYDIYRYFSFFLLTYVFLFFLFISFSRLLLMLFWWGFSRDDITFTDPLNTFTGIERYKLIFWALRFHGKILFREIGIEVYRIWQPSENVILIRWNLKGVPRVPWEARGEFQGTSRYKVDRNGKIYEHKVDNLAFNFPQQLKPAASVLDLVSACPASPNPTFLWGTEDLHCSSWVELYQSVRRSVGGEGYLITQDGFLTCS*
Homology
BLAST of Chy3G050280 vs. ExPASy Swiss-Prot
Match: O67550 (5'-3' exonuclease OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1628 PE=3 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 4.7e-24
Identity = 81/289 (28.03%), Postives = 145/289 (50.17%), Query Frame = 0

Query: 89  FLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSHSDP--VIAVFDGEGGSEHRRLLLPSYKA 148
           F  + PL      P+   +G ++ + F  +    P  ++ VFD    ++ R  +   YK 
Sbjct: 17  FFALPPLSTSKGFPTNAIYG-FLRMLFSLIKKERPQYLVVVFDAPAKTK-REKIYADYKK 76

Query: 149 HRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCNVPVVRVEGHEADDVIATLVEQVLQRG 208
            R K   P           +    VI++ L+   +P++ + G+EADDVIA L E+  Q+G
Sbjct: 77  QRPKAPDP----------LKVQIPVIKEILKLAGIPLLELPGYEADDVIAYLAEKFSQKG 136

Query: 209 VRVVVASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCDPCSDLSLRCIMGD 268
            +V + SPDKD  QL+SE+V ++ P+ +      +T    + ++  +P        ++GD
Sbjct: 137 FKVKIYSPDKDLLQLVSENVLVINPMND----EVFTKERVIKKFGVEPQKIPDYLALVGD 196

Query: 269 EVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLLSAAAIRTVGKPYAQDALTKYAEYLRT 328
           +VD VPGI+    G G KTA+ +LKK+GS+EN+L         + + ++      E L  
Sbjct: 197 KVDNVPGIE----GVGPKTAINILKKYGSVENILKN------WEKFQREFPRAKKEDLEL 256

Query: 329 NYRVLALRRDVDVQFQDEWLVERDRRNDSTILSKFVENNDRNLLVQPSK 376
           +Y+++ L  D+D++  +E L +  R + + +  K  E   ++LL +  K
Sbjct: 257 SYKLVKLYTDLDIELSEEDL-KIKRPDLNKLKQKLQELEMKSLLKEVDK 278

BLAST of Chy3G050280 vs. ExPASy Swiss-Prot
Match: Q04957 (DNA polymerase I OS=Bacillus caldotenax OX=1395 GN=polA PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 3.1e-20
Identity = 80/281 (28.47%), Postives = 130/281 (46.26%), Query Frame = 0

Query: 116 EEVSHSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDA 175
           EE +H   ++  FD  G +  R      YK  R + T P  S++F          ++R+ 
Sbjct: 49  EEPTH---MLVAFDA-GKTTFRHEAFQEYKGGR-QQTPPELSEQF---------PLLREL 108

Query: 176 LRSCNVPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPEL 235
           LR+  +P   +E +EADD+I TL  +  Q G  V V S D+D  QL S  V + +    +
Sbjct: 109 LRAYRIPAYELENYEADDIIGTLAARAEQEGFEVKVISGDRDLTQLASPHVTVDITKKGI 168

Query: 236 NRWSFYTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGS 295
                YT      +Y   P   + L+ +MGD+ D +PG+    PG G KTA+KLL++ G+
Sbjct: 169 TDIEPYTPEAVREKYGLTPEQIVDLKGLMGDKSDNIPGV----PGIGEKTAVKLLRQFGT 228

Query: 296 LENLLSAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDS 355
           +EN+L  A+I  +     ++ L ++ E    + ++ A+RRD  V+   + +         
Sbjct: 229 VENVL--ASIDEIKGEKLKETLRQHREMALLSKKLAAIRRDAPVELSLDDIA-------- 288

Query: 356 TILSKFVENNDRNLLVQPSKQLQGSQLASPPGSPDSRSDKP 397
                  +  DR  +V   K+L          SP S  +KP
Sbjct: 289 ------YQGEDREKVVALFKELGFQSFLEKMESPSSEEEKP 295

BLAST of Chy3G050280 vs. ExPASy Swiss-Prot
Match: Q9RLB6 (DNA polymerase I OS=Rickettsia helvetica OX=35789 GN=polA PE=3 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 3.5e-19
Identity = 64/219 (29.22%), Postives = 107/219 (48.86%), Query Frame = 0

Query: 124 VIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCNVPV 183
           V  VFD  GG   R  + P YKA+     RPP  +     +      ++RD   + N P+
Sbjct: 56  VAVVFD-SGGKNFRHYIYPEYKAN-----RPPPPE-----DLIVQLPLVRDVASNLNFPI 115

Query: 184 VRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSFYTL 243
           +   G+EADD+IAT   +    G  VV+ S DKD  QL+SE++++  PL    R  + T 
Sbjct: 116 LEKNGYEADDIIATFATKTAALGENVVIISSDKDLLQLMSENIKIYDPL----RGKYITE 175

Query: 244 RHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLLSAA 303
              + ++         +  ++GD  D +PG+    P  G KTA  L+ + GS+EN+ +  
Sbjct: 176 DDVVKKFGTTSDKLREVMALIGDRSDNIPGV----PSIGPKTASSLITQFGSVENIFN-- 235

Query: 304 AIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQ 343
           ++  V     ++ L    E    +++++ L  +VD+ FQ
Sbjct: 236 SLDQVSSVKQRETLQNSREAALISWQLIGLDSNVDLDFQ 253

BLAST of Chy3G050280 vs. ExPASy Swiss-Prot
Match: O08307 (DNA polymerase I OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl) OX=324602 GN=polA PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 5.9e-19
Identity = 78/263 (29.66%), Postives = 121/263 (46.01%), Query Frame = 0

Query: 85  RRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSHSDPVIAVFDGEGGSEHRRLLLPSY 144
           R  F L  + L     +P+   FG +  I    ++   P  A    + G   R  L   Y
Sbjct: 17  RAFFALRESGLRSSRGEPTYAVFG-FAQILLTALAEYRPDYAAVAFDVGRTFRDDLYAEY 76

Query: 145 KAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCNVPVVRVEGHEADDVIATLVEQVLQ 204
           KA R +          T   F   ++ I+  +++ N+P+   EG+EADDVI TL  Q  +
Sbjct: 77  KAGRAE----------TPEEFYPQFERIKQLVQALNIPIYTAEGYEADDVIGTLARQATE 136

Query: 205 RGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWS--FYTLRHYLAQYN-CDPCSDLSLR 264
           RGV  ++ + D D  QL+++ V++ +  P   + S   Y L     +Y+  +P     LR
Sbjct: 137 RGVDTIILTGDSDVLQLVNDHVRVALANPYGGKTSVTLYDLEQVRKRYDGLEPDQLADLR 196

Query: 265 CIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLLSAAAIRTVGKPYAQDALTKYA 324
            + GD  D +PG++    G G K A+ LLK+  SL+ LL    I    K Y Q  L + A
Sbjct: 197 GLKGDTSDNIPGVR----GIGEKGAIALLKQFRSLDALLE--QIDAAPKRY-QTLLREQA 256

Query: 325 EYLRTNYRVLALRRDVDVQFQDE 345
           E  R + ++  +  DV VQ   E
Sbjct: 257 EAARFSRQLATIVTDVPVQLDLE 261

BLAST of Chy3G050280 vs. ExPASy Swiss-Prot
Match: Q92GB7 (DNA polymerase I OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944 GN=polA PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 1.0e-18
Identity = 62/219 (28.31%), Postives = 107/219 (48.86%), Query Frame = 0

Query: 124 VIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCNVPV 183
           V  VFD  GG   R  + P YKA+     RPP  +     +      ++RD   + N P+
Sbjct: 56  VAVVFD-SGGKNFRHQIYPDYKAN-----RPPPPE-----DLIIQLPLVRDVASNLNFPI 115

Query: 184 VRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSFYTL 243
           +   G+EADD+IAT   +    G  VV+ S DKD  QL++E++++  PL    +  + T 
Sbjct: 116 LEKNGYEADDIIATFATKTAALGAHVVIISSDKDLLQLMTENIKIYDPL----KGKYITE 175

Query: 244 RHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLLSAA 303
              + ++         +  ++GD  D +PG+    P  G KTA  L+ + GS+EN+ +  
Sbjct: 176 DDVVKKFGTTSDKLREVMALIGDRSDNIPGV----PSIGPKTASSLITQFGSVENIFN-- 235

Query: 304 AIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQ 343
           ++  V     ++ L    E    +++++ L  +VD+ FQ
Sbjct: 236 SLDQVSSVKQRETLQNSREAALISWQLIGLNSNVDLDFQ 253

BLAST of Chy3G050280 vs. ExPASy TrEMBL
Match: A0A0A0LQN4 (53EXOc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G402050 PE=4 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 5.3e-212
Identity = 369/377 (97.88%), Postives = 373/377 (98.94%), Query Frame = 0

Query: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPESPLTSKLKPNTWRTKPLNLTAFAPFSRL 60
           MAEASANIGVNFPPFLNSSSPTFLPSRTLKPE PLTSKLKPNTWRTKPLNLTAFAP SRL
Sbjct: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPEFPLTSKLKPNTWRTKPLNLTAFAPSSRL 60

Query: 61  TSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120
           TSAAF QTDDGKFQPRIEADNSRT RVFFLDVNPLCYQG+KPSLRNFGRWVSIFFEEVSH
Sbjct: 61  TSAAFTQTDDGKFQPRIEADNSRTGRVFFLDVNPLCYQGSKPSLRNFGRWVSIFFEEVSH 120

Query: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180
           SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN
Sbjct: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180

Query: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240
           VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISED+QLVMPLPELNRWSF
Sbjct: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDIQLVMPLPELNRWSF 240

Query: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300
           YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL
Sbjct: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300

Query: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360
           SAAAIRTVGKPYAQDALTKYAEYLRTNY+VLALRRDVDVQFQDEWLVERDRRNDSTILSK
Sbjct: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360

Query: 361 FVENNDRNLLVQPSKQL 378
           FVENNDRNLLVQPSKQ+
Sbjct: 361 FVENNDRNLLVQPSKQV 377

BLAST of Chy3G050280 vs. ExPASy TrEMBL
Match: A0A1S3B2X7 (5'-3' exonuclease OS=Cucumis melo OX=3656 GN=LOC103485414 PE=4 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 2.2e-202
Identity = 355/377 (94.16%), Postives = 362/377 (96.02%), Query Frame = 0

Query: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPESPLTSKLKPNTWRTKPLNLTAFAPFSRL 60
           M EASA IGVNFPPFLNSSS TFLPSRT      LTSKLKPNTWRTKPL LTAF P SRL
Sbjct: 1   MVEASAKIGVNFPPFLNSSSRTFLPSRT-----ALTSKLKPNTWRTKPLKLTAFVPSSRL 60

Query: 61  TSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120
           TSAAFNQTDDGKFQPRIEADN R  RVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH
Sbjct: 61  TSAAFNQTDDGKFQPRIEADNPRKGRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120

Query: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180
           SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSS+RFTKGNFRTSYQVIRDALRSCN
Sbjct: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSQRFTKGNFRTSYQVIRDALRSCN 180

Query: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240
           VPVV+V+GHEADDV+ATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF
Sbjct: 181 VPVVKVDGHEADDVVATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240

Query: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300
           YT+RHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL
Sbjct: 241 YTIRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300

Query: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360
           SAAAIRTVGKPYAQDALTKYAEYLRTNY+VLALRRDVDVQFQDEWLVERDRRNDSTILSK
Sbjct: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360

Query: 361 FVENNDRNLLVQPSKQL 378
           FVENNDRNLLVQPSKQ+
Sbjct: 361 FVENNDRNLLVQPSKQV 372

BLAST of Chy3G050280 vs. ExPASy TrEMBL
Match: A0A5A7T6A6 (5'-3' exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G001120 PE=4 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 2.2e-202
Identity = 355/377 (94.16%), Postives = 362/377 (96.02%), Query Frame = 0

Query: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPESPLTSKLKPNTWRTKPLNLTAFAPFSRL 60
           M EASA IGVNFPPFLNSSS TFLPSRT      LTSKLKPNTWRTKPL LTAF P SRL
Sbjct: 1   MVEASAKIGVNFPPFLNSSSRTFLPSRT-----ALTSKLKPNTWRTKPLKLTAFVPSSRL 60

Query: 61  TSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120
           TSAAFNQTDDGKFQPRIEADN R  RVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH
Sbjct: 61  TSAAFNQTDDGKFQPRIEADNPRKGRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120

Query: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180
           SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSS+RFTKGNFRTSYQVIRDALRSCN
Sbjct: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSQRFTKGNFRTSYQVIRDALRSCN 180

Query: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240
           VPVV+V+GHEADDV+ATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF
Sbjct: 181 VPVVKVDGHEADDVVATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240

Query: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300
           YT+RHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL
Sbjct: 241 YTIRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300

Query: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360
           SAAAIRTVGKPYAQDALTKYAEYLRTNY+VLALRRDVDVQFQDEWLVERDRRNDSTILSK
Sbjct: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360

Query: 361 FVENNDRNLLVQPSKQL 378
           FVENNDRNLLVQPSKQ+
Sbjct: 361 FVENNDRNLLVQPSKQV 372

BLAST of Chy3G050280 vs. ExPASy TrEMBL
Match: A0A6J1JHT6 (uncharacterized protein LOC111487120 OS=Cucurbita maxima OX=3661 GN=LOC111487120 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.7e-178
Identity = 314/373 (84.18%), Postives = 340/373 (91.15%), Query Frame = 0

Query: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLK-PESPLTSKLKPNTWRTKPLNLTAFAPFSR 60
           MAEASANIG+N PPFLNS+SPT LPSRTLK  ES  T+K K  +WRTKPL L+ FA  SR
Sbjct: 1   MAEASANIGLNIPPFLNSASPTSLPSRTLKAAESVRTTKAK--SWRTKPLKLSVFAAASR 60

Query: 61  LTSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVS 120
            TS+ F Q +DGK  PR+EADN R  RVFFLDVNPLCYQG++PSL NFGRWVSIFFEEVS
Sbjct: 61  STSSGFKQAEDGKLPPRMEADNPRKGRVFFLDVNPLCYQGSRPSLHNFGRWVSIFFEEVS 120

Query: 121 HSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSC 180
           HSDPVIAV DGEGGSEHRRLLLPSYKAHRIKFTR  SS+RFTKGN   SYQVIRDALRSC
Sbjct: 121 HSDPVIAVIDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRFTKGNSGRSYQVIRDALRSC 180

Query: 181 NVPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWS 240
           +VPV++++GHEADDV+ATLVEQVLQRG R V+ASPDKDFKQLISEDVQLVMPLPELNRWS
Sbjct: 181 DVPVIKIKGHEADDVVATLVEQVLQRGFRAVIASPDKDFKQLISEDVQLVMPLPELNRWS 240

Query: 241 FYTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENL 300
           FYTL+HYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTA+KLLKKHGSLENL
Sbjct: 241 FYTLKHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENL 300

Query: 301 LSAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILS 360
           LSAAAIRTVGKPYAQDALTKYA+YLRTNY+VLALRRDVDVQF++EWLVERDR+NDSTILS
Sbjct: 301 LSAAAIRTVGKPYAQDALTKYADYLRTNYKVLALRRDVDVQFREEWLVERDRQNDSTILS 360

Query: 361 KFVENNDRNLLVQ 373
           KFVENNDRN L +
Sbjct: 361 KFVENNDRNSLAE 371

BLAST of Chy3G050280 vs. ExPASy TrEMBL
Match: A0A6J1DGI1 (uncharacterized protein LOC111020233 OS=Momordica charantia OX=3673 GN=LOC111020233 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 1.7e-178
Identity = 316/377 (83.82%), Postives = 341/377 (90.45%), Query Frame = 0

Query: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPESPLTSKLKPNTWRTKPLNLTAFAPFSRL 60
           MAEA ANIGVN PPFLNS+S + LPSRTLKPES LT+  K N+WRTK L L+AF   S  
Sbjct: 1   MAEAGANIGVNIPPFLNSASRSSLPSRTLKPESTLTT--KSNSWRTKALQLSAFPVASHS 60

Query: 61  TSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120
           T   F QTD G  QP IEADN R  RVFFLDVNPLCY+G++PSL NFGRW SIFFE+VSH
Sbjct: 61  T---FKQTDGGTLQPIIEADNPRKGRVFFLDVNPLCYEGSRPSLHNFGRWASIFFEQVSH 120

Query: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180
           SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTR  SS+R+TKGN R  YQVIRDALR+CN
Sbjct: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRYTKGNSRRPYQVIRDALRNCN 180

Query: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240
           VPVV+V+GHEADDV+ATLV+QVLQRG RVV+ASPDKDFKQLISEDVQLVMPLPELNRWSF
Sbjct: 181 VPVVKVDGHEADDVVATLVDQVLQRGFRVVIASPDKDFKQLISEDVQLVMPLPELNRWSF 240

Query: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300
           YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTA+KLLKKHGSLENLL
Sbjct: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENLL 300

Query: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360
           SAAAIRTVG+PYAQDALTKYA+YLRTNY+VLALRRDVDVQFQ+EWLVERDR+NDS ILSK
Sbjct: 301 SAAAIRTVGRPYAQDALTKYADYLRTNYKVLALRRDVDVQFQEEWLVERDRQNDSAILSK 360

Query: 361 FVENNDRNLLVQPSKQL 378
           FVENNDRN LVQPSK++
Sbjct: 361 FVENNDRNSLVQPSKRV 372

BLAST of Chy3G050280 vs. NCBI nr
Match: KAG6602717.1 (hypothetical protein SDJN03_07950, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 949 bits (2454), Expect = 0.0
Identity = 486/669 (72.65%), Postives = 526/669 (78.62%), Query Frame = 0

Query: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKP-ESPLTSKLKPNTWRTKPLNLTAFAPFSR 60
           MAEASAN+G+N PPFLNS+SPT LPSRTLK  ES  T+K KP  WRTKPL L+ FA  SR
Sbjct: 1   MAEASANLGLNIPPFLNSTSPTSLPSRTLKAAESVRTTKAKP--WRTKPLKLSVFAASSR 60

Query: 61  LTSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVS 120
            TS+ F Q +DGK  P +EADN R  RVFFLDVNPLCYQG++PSL NFGRWVSIFFEEVS
Sbjct: 61  STSSGFKQAEDGKLPPTVEADNPRKGRVFFLDVNPLCYQGSRPSLHNFGRWVSIFFEEVS 120

Query: 121 HSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSC 180
            SDPVIAV DGEGGSEHRRLLLPSYKAHRIKFTR  SS+RFTKGN   SYQVIRDALRSC
Sbjct: 121 RSDPVIAVIDGEGGSEHRRLLLPSYKAHRIKFTRQSSSQRFTKGNSGRSYQVIRDALRSC 180

Query: 181 NVPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWS 240
           NVPV++++GHEADDV+ATLVEQVLQRG R V+ASPDKDFKQLISEDVQLVMPLPELNRWS
Sbjct: 181 NVPVIKIKGHEADDVVATLVEQVLQRGFRAVIASPDKDFKQLISEDVQLVMPLPELNRWS 240

Query: 241 FYTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENL 300
           FYTL+HYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTA+KLLKKHGSLENL
Sbjct: 241 FYTLKHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTAMKLLKKHGSLENL 300

Query: 301 LSAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILS 360
           LSAAA+RTVGKPYAQDALTKYA+YLRTNY+VLALRRD+D    +  L   +   +   L+
Sbjct: 301 LSAAAVRTVGKPYAQDALTKYADYLRTNYKVLALRRDIDKTMTETHLPNNETSVNPP-LN 360

Query: 361 KFVE-----------------------------------------NNDRNL----LVQPS 420
           ++++                                         ++  +L    L  P 
Sbjct: 361 EYIQGEKCVIPFLLIHCGIKANPYAIEGLTGTFICAGRTMWASSSSSSSSLPLTSLHSPQ 420

Query: 421 KQLQGSQLASPPGSPDSRSDKPRDDFYVNLGLAVRTLREDLPLIFTRDLNYDIYRYFSFF 480
             LQG QLASP   PDSRSD  RDDFYVNLGLAVRTLREDLPLIF RDLNYDIYR     
Sbjct: 421 PLLQGPQLASPSAFPDSRSDDRRDDFYVNLGLAVRTLREDLPLIFARDLNYDIYR----- 480

Query: 481 LLTYVFLFFLFISFSRLLLMLFWWGFSRDDITFTDPLNTFTGIERYKLIFWALRFHGKIL 540
                                       DDITF DPLNTF+GIERYKLIFWALRFHG+IL
Sbjct: 481 ----------------------------DDITFIDPLNTFSGIERYKLIFWALRFHGRIL 540

Query: 541 FREIGIEVYRIWQPSENVILIRWNLKGVPRVPWEARGEFQGTSRYKVDRNGKIYEHKVDN 600
           FREIGIEVYRIWQPSENVILIRWNLKGVPRVPWEARGEFQGTSR+KVDRNGKIYEHKVDN
Sbjct: 541 FREIGIEVYRIWQPSENVILIRWNLKGVPRVPWEARGEFQGTSRFKVDRNGKIYEHKVDN 600

Query: 601 LAFNFPQQLKPAASVLDLVSACPASPNPTFLWGTEDLHCSSWVELYQSVRRSVGGEGYLI 623
           LAFNFPQ LKPAASVLDLV+ACPASPNPTF WGTE+LHCSSWVELYQ+VRRSVGGEGYLI
Sbjct: 601 LAFNFPQSLKPAASVLDLVTACPASPNPTFFWGTEELHCSSWVELYQAVRRSVGGEGYLI 633

BLAST of Chy3G050280 vs. NCBI nr
Match: XP_004138652.1 (uncharacterized protein LOC101219234 [Cucumis sativus] >XP_011649896.1 uncharacterized protein LOC101219234 [Cucumis sativus] >KGN63097.1 hypothetical protein Csa_022634 [Cucumis sativus])

HSP 1 Score: 743 bits (1918), Expect = 3.62e-266
Identity = 369/377 (97.88%), Postives = 373/377 (98.94%), Query Frame = 0

Query: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPESPLTSKLKPNTWRTKPLNLTAFAPFSRL 60
           MAEASANIGVNFPPFLNSSSPTFLPSRTLKPE PLTSKLKPNTWRTKPLNLTAFAP SRL
Sbjct: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPEFPLTSKLKPNTWRTKPLNLTAFAPSSRL 60

Query: 61  TSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120
           TSAAF QTDDGKFQPRIEADNSRT RVFFLDVNPLCYQG+KPSLRNFGRWVSIFFEEVSH
Sbjct: 61  TSAAFTQTDDGKFQPRIEADNSRTGRVFFLDVNPLCYQGSKPSLRNFGRWVSIFFEEVSH 120

Query: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180
           SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN
Sbjct: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180

Query: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240
           VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISED+QLVMPLPELNRWSF
Sbjct: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDIQLVMPLPELNRWSF 240

Query: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300
           YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL
Sbjct: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300

Query: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360
           SAAAIRTVGKPYAQDALTKYAEYLRTNY+VLALRRDVDVQFQDEWLVERDRRNDSTILSK
Sbjct: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360

Query: 361 FVENNDRNLLVQPSKQL 377
           FVENNDRNLLVQPSKQ+
Sbjct: 361 FVENNDRNLLVQPSKQV 377

BLAST of Chy3G050280 vs. NCBI nr
Match: XP_008441212.1 (PREDICTED: 5'-3' exonuclease [Cucumis melo] >XP_008441213.1 PREDICTED: 5'-3' exonuclease [Cucumis melo])

HSP 1 Score: 711 bits (1836), Expect = 8.88e-254
Identity = 355/377 (94.16%), Postives = 362/377 (96.02%), Query Frame = 0

Query: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPESPLTSKLKPNTWRTKPLNLTAFAPFSRL 60
           M EASA IGVNFPPFLNSSS TFLPSRT      LTSKLKPNTWRTKPL LTAF P SRL
Sbjct: 1   MVEASAKIGVNFPPFLNSSSRTFLPSRTA-----LTSKLKPNTWRTKPLKLTAFVPSSRL 60

Query: 61  TSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120
           TSAAFNQTDDGKFQPRIEADN R  RVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH
Sbjct: 61  TSAAFNQTDDGKFQPRIEADNPRKGRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120

Query: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180
           SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSS+RFTKGNFRTSYQVIRDALRSCN
Sbjct: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSQRFTKGNFRTSYQVIRDALRSCN 180

Query: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240
           VPVV+V+GHEADDV+ATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF
Sbjct: 181 VPVVKVDGHEADDVVATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240

Query: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300
           YT+RHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL
Sbjct: 241 YTIRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300

Query: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360
           SAAAIRTVGKPYAQDALTKYAEYLRTNY+VLALRRDVDVQFQDEWLVERDRRNDSTILSK
Sbjct: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360

Query: 361 FVENNDRNLLVQPSKQL 377
           FVENNDRNLLVQPSKQ+
Sbjct: 361 FVENNDRNLLVQPSKQV 372

BLAST of Chy3G050280 vs. NCBI nr
Match: KAA0037015.1 (5'-3' exonuclease [Cucumis melo var. makuwa] >TYK06635.1 5'-3' exonuclease [Cucumis melo var. makuwa])

HSP 1 Score: 711 bits (1836), Expect = 3.98e-253
Identity = 355/377 (94.16%), Postives = 362/377 (96.02%), Query Frame = 0

Query: 1   MAEASANIGVNFPPFLNSSSPTFLPSRTLKPESPLTSKLKPNTWRTKPLNLTAFAPFSRL 60
           M EASA IGVNFPPFLNSSS TFLPSRT      LTSKLKPNTWRTKPL LTAF P SRL
Sbjct: 1   MVEASAKIGVNFPPFLNSSSRTFLPSRTA-----LTSKLKPNTWRTKPLKLTAFVPSSRL 60

Query: 61  TSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120
           TSAAFNQTDDGKFQPRIEADN R  RVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH
Sbjct: 61  TSAAFNQTDDGKFQPRIEADNPRKGRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSH 120

Query: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCN 180
           SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSS+RFTKGNFRTSYQVIRDALRSCN
Sbjct: 121 SDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSQRFTKGNFRTSYQVIRDALRSCN 180

Query: 181 VPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240
           VPVV+V+GHEADDV+ATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF
Sbjct: 181 VPVVKVDGHEADDVVATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSF 240

Query: 241 YTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300
           YT+RHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL
Sbjct: 241 YTIRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLL 300

Query: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360
           SAAAIRTVGKPYAQDALTKYAEYLRTNY+VLALRRDVDVQFQDEWLVERDRRNDSTILSK
Sbjct: 301 SAAAIRTVGKPYAQDALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRRNDSTILSK 360

Query: 361 FVENNDRNLLVQPSKQL 377
           FVENNDRNLLVQPSKQ+
Sbjct: 361 FVENNDRNLLVQPSKQV 372

BLAST of Chy3G050280 vs. NCBI nr
Match: XP_038884032.1 (5'-3' exonuclease [Benincasa hispida])

HSP 1 Score: 667 bits (1720), Expect = 4.77e-236
Identity = 335/378 (88.62%), Postives = 351/378 (92.86%), Query Frame = 0

Query: 1   MAEASANIGV-NFPPFLNSSSPTFLPSRTLKPESPLTSKLKPNTWRTKPLNLTAFAPFSR 60
           MAEASANIGV N PPFLNSSS T LPSRTLK E+ +TSKLK NTWRTKPL LT FA  SR
Sbjct: 1   MAEASANIGVLNLPPFLNSSSRTSLPSRTLKAEAAVTSKLKANTWRTKPLKLTVFAASSR 60

Query: 61  LTSAAFNQTDDGKFQPRIEADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVS 120
            TSAAFNQTD GKFQPRIEADN R  RVFFLDVNPLCYQGN+PSL NFGRW+SIFFEEVS
Sbjct: 61  YTSAAFNQTDCGKFQPRIEADNPRNGRVFFLDVNPLCYQGNRPSLHNFGRWLSIFFEEVS 120

Query: 121 HSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSC 180
           HSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTR PSS+RFTKGN R SYQVIRDALR+C
Sbjct: 121 HSDPVIAVFDGEGGSEHRRLLLPSYKAHRIKFTRQPSSQRFTKGNSRRSYQVIRDALRNC 180

Query: 181 NVPVVRVEGHEADDVIATLVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWS 240
           NVPVV+V+G EADDV+ATLVEQVLQRGVRVV+ASPDKDFKQLISEDVQLVMPLPELNRWS
Sbjct: 181 NVPVVKVDGQEADDVVATLVEQVLQRGVRVVIASPDKDFKQLISEDVQLVMPLPELNRWS 240

Query: 241 FYTLRHYLAQYNCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENL 300
           FYTLRHYLAQY+CDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENL
Sbjct: 241 FYTLRHYLAQYHCDPCSDLSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENL 300

Query: 301 LSAAAIRTVGKPYAQDALTKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILS 360
           LSAAAIRTVGKPYAQ ALTKYAEYLRTNY+VLALRRDVDVQFQDEWLVERDR+ND  ILS
Sbjct: 301 LSAAAIRTVGKPYAQVALTKYAEYLRTNYKVLALRRDVDVQFQDEWLVERDRQNDLAILS 360

Query: 361 KFVENNDRNLLVQPSKQL 377
           KFVEN +RN L QPSK++
Sbjct: 361 KFVENTERNSLAQPSKRV 378

BLAST of Chy3G050280 vs. TAIR 10
Match: AT1G34380.2 (5'-3' exonuclease family protein )

HSP 1 Score: 408.3 bits (1048), Expect = 1.1e-113
Identity = 194/284 (68.31%), Postives = 235/284 (82.75%), Query Frame = 0

Query: 78  EADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSHSDPVIAVFDGEGGSEHR 137
           E    + +RVFFLDV+PLCY+GNKPS + FG W+S+FF +VS +DPVIAV DGE G++ R
Sbjct: 66  EEIRKKNKRVFFLDVSPLCYEGNKPSSQAFGHWISLFFSQVSLTDPVIAVIDGEEGNQRR 125

Query: 138 RLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCNVPVVRVEGHEADDVIAT 197
           R LLPSYKAHR    + P+  R++K      +Q + + LR CNVPVVR+EGHEADDV+AT
Sbjct: 126 RELLPSYKAHR----KSPNHGRYSK----RPHQFVDEVLRKCNVPVVRIEGHEADDVVAT 185

Query: 198 LVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCDPCSD 257
           L+EQ +QRG R V+ASPDKDFKQLISE+VQ+V+PL +L RWSFYTL+HY AQYNCDP SD
Sbjct: 186 LMEQAVQRGYRAVIASPDKDFKQLISENVQIVIPLADLRRWSFYTLKHYHAQYNCDPQSD 245

Query: 258 LSLRCIMGDEVDGVPGIQHVAPGFGRKTALKLLKKHGSLENLLSAAAIRTVGKPYAQDAL 317
           LS RCIMGDEVDGVPGIQH+ P FGRKTA+KL++KHGSLE+LLSAAA+RTVG+PYAQ+AL
Sbjct: 246 LSFRCIMGDEVDGVPGIQHMVPAFGRKTAMKLVRKHGSLESLLSAAAVRTVGRPYAQEAL 305

Query: 318 TKYAEYLRTNYRVLALRRDVDVQFQDEWLVERDRRNDSTILSKF 362
           TKYA+YLR NY+VLAL RDV VQ Q+EWL+ERD  NDS +LS F
Sbjct: 306 TKYADYLRRNYQVLALNRDVRVQIQNEWLMERDTSNDSEVLSSF 341

BLAST of Chy3G050280 vs. TAIR 10
Match: AT1G79510.1 (Uncharacterized conserved protein (DUF2358) )

HSP 1 Score: 325.5 bits (833), Expect = 9.5e-89
Identity = 153/254 (60.24%), Postives = 188/254 (74.02%), Query Frame = 0

Query: 371 VQPSKQLQGSQLASPPGSPDSRSDKPRDDFYVNLGLAVRTLREDLPLIFTRDLNYDIYRY 430
           VQ    ++G+Q+ + P + D      +DDFY+NLGLAVRTLREDLPL+FT+DLNYDIY  
Sbjct: 55  VQAPPPVRGAQVKTKPSAQDKYQHGSKDDFYINLGLAVRTLREDLPLLFTKDLNYDIY-- 114

Query: 431 FSFFLLTYVFLFFLFISFSRLLLMLFWWGFSRDDITFTDPLNTFTGIERYKLIFWALRFH 490
                                          RDDIT  DP+NTF+GI+ YKLIFWALRFH
Sbjct: 115 -------------------------------RDDITLVDPMNTFSGIDNYKLIFWALRFH 174

Query: 491 GKILFREIGIEVYRIWQPSENVILIRWNLKGVPRVPWEARGEFQGTSRYKVDRNGKIYEH 550
           GKILFR+I +E++R+WQPSEN+ILIRWNLKGVPRVPWEA+GEFQGTSRYK+DRNGKIYEH
Sbjct: 175 GKILFRDISLEIFRVWQPSENMILIRWNLKGVPRVPWEAKGEFQGTSRYKLDRNGKIYEH 234

Query: 551 KVDNLAFNFPQQLKPAASVLDLVSACPASPNPTFLWGTEDLHCSSWVELYQSVRRSVG-G 610
           KVDNLAFNFP QLKPA SVLD+V+ACPASPNPTF++G  D + SSW+E Y++V+R++   
Sbjct: 235 KVDNLAFNFPHQLKPATSVLDMVTACPASPNPTFMFGAMDSYSSSWIEFYKAVQRTLDKQ 275

Query: 611 EGYLITQDGFLTCS 624
           E  ++ QD F+ CS
Sbjct: 295 EEQMLVQDHFVPCS 275

BLAST of Chy3G050280 vs. TAIR 10
Match: AT1G79510.2 (Uncharacterized conserved protein (DUF2358) )

HSP 1 Score: 325.5 bits (833), Expect = 9.5e-89
Identity = 153/254 (60.24%), Postives = 188/254 (74.02%), Query Frame = 0

Query: 371 VQPSKQLQGSQLASPPGSPDSRSDKPRDDFYVNLGLAVRTLREDLPLIFTRDLNYDIYRY 430
           VQ    ++G+Q+ + P + D      +DDFY+NLGLAVRTLREDLPL+FT+DLNYDIY  
Sbjct: 55  VQAPPPVRGAQVKTKPSAQDKYQHGSKDDFYINLGLAVRTLREDLPLLFTKDLNYDIY-- 114

Query: 431 FSFFLLTYVFLFFLFISFSRLLLMLFWWGFSRDDITFTDPLNTFTGIERYKLIFWALRFH 490
                                          RDDIT  DP+NTF+GI+ YKLIFWALRFH
Sbjct: 115 -------------------------------RDDITLVDPMNTFSGIDNYKLIFWALRFH 174

Query: 491 GKILFREIGIEVYRIWQPSENVILIRWNLKGVPRVPWEARGEFQGTSRYKVDRNGKIYEH 550
           GKILFR+I +E++R+WQPSEN+ILIRWNLKGVPRVPWEA+GEFQGTSRYK+DRNGKIYEH
Sbjct: 175 GKILFRDISLEIFRVWQPSENMILIRWNLKGVPRVPWEAKGEFQGTSRYKLDRNGKIYEH 234

Query: 551 KVDNLAFNFPQQLKPAASVLDLVSACPASPNPTFLWGTEDLHCSSWVELYQSVRRSVG-G 610
           KVDNLAFNFP QLKPA SVLD+V+ACPASPNPTF++G  D + SSW+E Y++V+R++   
Sbjct: 235 KVDNLAFNFPHQLKPATSVLDMVTACPASPNPTFMFGAMDSYSSSWIEFYKAVQRTLDKQ 275

Query: 611 EGYLITQDGFLTCS 624
           E  ++ QD F+ CS
Sbjct: 295 EEQMLVQDHFVPCS 275

BLAST of Chy3G050280 vs. TAIR 10
Match: AT1G16320.1 (Uncharacterized conserved protein (DUF2358) )

HSP 1 Score: 323.9 bits (829), Expect = 2.8e-88
Identity = 161/274 (58.76%), Postives = 197/274 (71.90%), Query Frame = 0

Query: 353 NDSTILSKFVENN--DRNLLVQPSKQLQGSQLASPPGSPDSRSDKPRDDFYVNLGLAVRT 412
           + S++  +FVE N    +LL   S  L+ +Q+ +   S D  ++  RD+FY+NLG+AVRT
Sbjct: 33  SSSSVSYEFVEENLSTLSLLSIQSPPLKDTQVQTRHSSQDKHNNHDRDEFYINLGVAVRT 92

Query: 413 LREDLPLIFTRDLNYDIYRYFSFFLLTYVFLFFLFISFSRLLLMLFWWGFSRDDITFTDP 472
           LREDLPL+FTRDLNYDIY                                 RDDITF DP
Sbjct: 93  LREDLPLLFTRDLNYDIY---------------------------------RDDITFVDP 152

Query: 473 LNTFTGIERYKLIFWALRFHGKILFREIGIEVYRIWQPSENVILIRWNLKGVPRVPWEAR 532
           +NTFTG++ YK+IFWALRFHGKILFR+I +E++R+WQPSEN+ILIRWNLKGVPRVPWEA+
Sbjct: 153 MNTFTGMDNYKIIFWALRFHGKILFRDISLEIFRVWQPSENMILIRWNLKGVPRVPWEAK 212

Query: 533 GEFQGTSRYKVDRNGKIYEHKVDNLAFNFPQQLKPAASVLDLVSACPA-SPNPTFLWGTE 592
           GEFQGTSRYK+DRNGKIYEHKVDNLAFNFPQQLKPAASVLDLV+A PA SPNPTF +   
Sbjct: 213 GEFQGTSRYKLDRNGKIYEHKVDNLAFNFPQQLKPAASVLDLVTASPASSPNPTFFFSPV 272

Query: 593 DLHCSSWVELYQSVRRSVGGEGYLITQDGFLTCS 624
           D + SSWV+ YQ+VR ++  E   +T D  +TCS
Sbjct: 273 DSYSSSWVKFYQAVRGTLETEDMFVTTDCLVTCS 273

BLAST of Chy3G050280 vs. TAIR 10
Match: AT1G34380.1 (5'-3' exonuclease family protein )

HSP 1 Score: 247.7 bits (631), Expect = 2.5e-65
Identity = 117/184 (63.59%), Postives = 145/184 (78.80%), Query Frame = 0

Query: 78  EADNSRTRRVFFLDVNPLCYQGNKPSLRNFGRWVSIFFEEVSHSDPVIAVFDGEGGSEHR 137
           E    + +RVFFLDV+PLCY+GNKPS + FG W+S+FF +VS +DPVIAV DGE G++ R
Sbjct: 66  EEIRKKNKRVFFLDVSPLCYEGNKPSSQAFGHWISLFFSQVSLTDPVIAVIDGEEGNQRR 125

Query: 138 RLLLPSYKAHRIKFTRPPSSKRFTKGNFRTSYQVIRDALRSCNVPVVRVEGHEADDVIAT 197
           R LLPSYKAHR    + P+  R++K      +Q + + LR CNVPVVR+EGHEADDV+AT
Sbjct: 126 RELLPSYKAHR----KSPNHGRYSK----RPHQFVDEVLRKCNVPVVRIEGHEADDVVAT 185

Query: 198 LVEQVLQRGVRVVVASPDKDFKQLISEDVQLVMPLPELNRWSFYTLRHYLAQYNCDPCSD 257
           L+EQ +QRG R V+ASPDKDFKQLISE+VQ+V+PL +L RWSFYTL+HY AQYNCDP SD
Sbjct: 186 LMEQAVQRGYRAVIASPDKDFKQLISENVQIVIPLADLRRWSFYTLKHYHAQYNCDPQSD 241

Query: 258 LSLR 262
           LS R
Sbjct: 246 LSFR 241

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O675504.7e-2428.035'-3' exonuclease OS=Aquifex aeolicus (strain VF5) OX=224324 GN=aq_1628 PE=3 SV=... [more]
Q049573.1e-2028.47DNA polymerase I OS=Bacillus caldotenax OX=1395 GN=polA PE=1 SV=1[more]
Q9RLB63.5e-1929.22DNA polymerase I OS=Rickettsia helvetica OX=35789 GN=polA PE=3 SV=1[more]
O083075.9e-1929.66DNA polymerase I OS=Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10... [more]
Q92GB71.0e-1828.31DNA polymerase I OS=Rickettsia conorii (strain ATCC VR-613 / Malish 7) OX=272944... [more]
Match NameE-valueIdentityDescription
A0A0A0LQN45.3e-21297.8853EXOc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G402050 PE=4... [more]
A0A1S3B2X72.2e-20294.165'-3' exonuclease OS=Cucumis melo OX=3656 GN=LOC103485414 PE=4 SV=1[more]
A0A5A7T6A62.2e-20294.165'-3' exonuclease OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00... [more]
A0A6J1JHT61.7e-17884.18uncharacterized protein LOC111487120 OS=Cucurbita maxima OX=3661 GN=LOC111487120... [more]
A0A6J1DGI11.7e-17883.82uncharacterized protein LOC111020233 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
Match NameE-valueIdentityDescription
KAG6602717.10.072.65hypothetical protein SDJN03_07950, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_004138652.13.62e-26697.88uncharacterized protein LOC101219234 [Cucumis sativus] >XP_011649896.1 uncharact... [more]
XP_008441212.18.88e-25494.16PREDICTED: 5'-3' exonuclease [Cucumis melo] >XP_008441213.1 PREDICTED: 5'-3' exo... [more]
KAA0037015.13.98e-25394.165'-3' exonuclease [Cucumis melo var. makuwa] >TYK06635.1 5'-3' exonuclease [Cucu... [more]
XP_038884032.14.77e-23688.625'-3' exonuclease [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT1G34380.21.1e-11368.315'-3' exonuclease family protein [more]
AT1G79510.19.5e-8960.24Uncharacterized conserved protein (DUF2358) [more]
AT1G79510.29.5e-8960.24Uncharacterized conserved protein (DUF2358) [more]
AT1G16320.12.8e-8858.76Uncharacterized conserved protein (DUF2358) [more]
AT1G34380.12.5e-6563.595'-3' exonuclease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0024215'-3' exonucleaseSMARTSM0047553exo3coord: 85..349
e-value: 7.7E-27
score: 105.2
IPR020045DNA polymerase I-like, H3TH domainPFAMPF013675_3_exonuccoord: 260..343
e-value: 2.9E-13
score: 50.2
IPR020045DNA polymerase I-like, H3TH domainCDDcd09898H3TH_53EXOcoord: 263..333
e-value: 8.04078E-15
score: 67.4241
NoneNo IPR availableGENE3D1.10.150.20coord: 250..331
e-value: 2.2E-18
score: 68.1
NoneNo IPR availableGENE3D3.40.50.1010coord: 77..249
e-value: 1.0E-28
score: 101.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 376..397
NoneNo IPR availablePANTHERPTHR10133:SF525'-3' EXONUCLEASE FAMILY PROTEINcoord: 14..367
NoneNo IPR availableCDDcd09859PIN_53EXOcoord: 122..231
e-value: 6.18811E-26
score: 102.058
IPR018790Protein of unknown function DUF2358PFAMPF10184DUF2358coord: 462..550
e-value: 7.9E-28
score: 97.0
IPR0200465'-3' exonuclease, alpha-helical arch, N-terminalPFAMPF027395_3_exonuc_Ncoord: 113..230
e-value: 9.6E-23
score: 80.8
IPR002298DNA polymerase APANTHERPTHR10133DNA POLYMERASE Icoord: 14..367
IPR0362795'-3' exonuclease, C-terminal domain superfamilySUPERFAMILY478075' to 3' exonuclease, C-terminal subdomaincoord: 260..359
IPR032710NTF2-like domain superfamilySUPERFAMILY54427NTF2-likecoord: 462..551
IPR029060PIN-like domain superfamilySUPERFAMILY88723PIN domain-likecoord: 85..232

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy3G050280.1Chy3G050280.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:0006261 DNA-dependent DNA replication
biological_process GO:0006302 double-strand break repair
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
molecular_function GO:0003677 DNA binding
molecular_function GO:0003887 DNA-directed DNA polymerase activity
molecular_function GO:0004527 exonuclease activity
molecular_function GO:0003824 catalytic activity