Chy2G047490 (gene) Cucumber (hystrix) v1

BLAST of Chy2G047490 vs. ExPASy Swiss-Prot
Match: Q9FLR0 (Protein NEN1 OS=Arabidopsis thaliana OX=3702 GN=NEN1 PE=2 SV=1)

HSP 1 Score: 526.6 bits (1355), Expect = 3.2e-148
Identity = 285/504 (56.55%), Postives = 353/504 (70.04%), Query Frame = 0

Query: 5   DDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLISSLS 64
           +DRSEIAFFDVETTVP R GQ+F+ILEFG+ILVCPKKL EL SY+TL++P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPAP 124
           VRCNGI RD V+ +P FA IAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   P P
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KG IDSL LLTQ+FGRRAGDMKMATLA YFG+G QTHRSLDDVRMNLEVLKYCATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 185 SSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQGGTHP 244
           SSLP    +NS VSP  ++  R   +S +GN                             
Sbjct: 184 SSLPYAHVDNS-VSPETISSRRRIDASREGN----------------------------- 243

Query: 245 ILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGAT-LETDINMEEHATVSTEASP 304
                T ++  R  +++E SA + D FNM  + +++     +++DI MEE     ++   
Sbjct: 244 -----TVTTSVRLPSISENSAAQPDPFNMSVLRNEMASDNHIQSDILMEEEQIEPSDVVA 303

Query: 305 SEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQ--RIQLWHKDDCLQLLCNNLRVRF 364
           SE+TS       FL PD +S+S I A   PF+ GSQ  +++L H D  LQL C+ L++RF
Sbjct: 304 SENTSDH---EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKIRF 363

Query: 365 GISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKSGYF 424
           G++ KF D  GR RL+FVVD+ P+L ++LEA D  AQ+L  DSGS SEW P V    G+ 
Sbjct: 364 GVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGSTSEWNPVVNPMKGFV 423

Query: 425 NYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFDAAELDSLIKPAAILDAFI 484
           NYP  R+HI T ++GD A YATE++Q+E+SGA Q+LIFS  +  EL+SL+   +++DAF+
Sbjct: 424 NYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEELESLLTTGSVVDAFL 468

Query: 485 SLDTYDYQQSAGIRLVAKKLIIHS 506
           SL+ YDYQQ AGIRLVAKKL+I S
Sbjct: 484 SLEPYDYQQKAGIRLVAKKLVIQS 468

BLAST of Chy2G047490 vs. ExPASy Swiss-Prot
Match: Q0V842 (Protein NEN2 OS=Arabidopsis thaliana OX=3702 GN=NEN2 PE=2 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 3.9e-146
Identity = 287/513 (55.95%), Postives = 353/513 (68.81%), Query Frame = 0

Query: 3   PTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLISS 62
           P +DRSEIAFFDVETT+P R GQ ++ILEFG+ILVCPKKLVEL++YS L++P++L+LI+ 
Sbjct: 9   PAEDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITP 68

Query: 63  LSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAP 122
            SV+CNGI R+ V S+ TFA IAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   P
Sbjct: 69  RSVKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPP 128

Query: 123 APKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLF 182
            PKGTIDSLALLTQRFGRRAGDMKMATLA+YFG+G QTHRSLDDVRMN EVLKYCATVLF
Sbjct: 129 EPKGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLF 188

Query: 183 LESSLPEVFPENSWVSPNAVT---RHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQ 242
           LESSLP+   ENS  +    T   R R  K SP                     S TDQQ
Sbjct: 189 LESSLPDELIENSVTTTTPETSSRRRRTIKKSPLQ-------------------SPTDQQ 248

Query: 243 GG----THPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINMEEHA 302
            G    T PILS V+ +              ++D F+M T+ ++I    L++D+ MEE  
Sbjct: 249 TGENMTTIPILSFVSSAE------------AQTDPFDMSTLRNEIAPEVLQSDVPMEEEQ 308

Query: 303 TVSTEASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQ--RIQLWHKDDCLQLL 362
              +E   SE T        F+E D++SVS I A+ VP + GSQ  ++QL+  D  LQL 
Sbjct: 309 NQQSETVASEGTGDQ---EGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLH 368

Query: 363 CNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPA 422
           C  L+VRFGI+ KF D AGR RL+FV+D+ P+LC VL+  D  AQ +  DSGSGS+W P 
Sbjct: 369 CPRLKVRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDWNPL 428

Query: 423 VTRKSGYFNYPTMRLHIPTAVSGDVANYATEMYQKEASG--AVQRLIFSKFDAAELDSLI 482
           V    G+ N PT R+HIPT ++GD+  YA E++QKE SG  A Q+LI S   A E++SL+
Sbjct: 429 VIPMKGFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIESLL 487

Query: 483 KPAAILDAFISLDTYDYQQSAGIRLVAKKLIIH 505
            P  +LDAF+SL+ YDYQQ AGIRLVA+KL+IH
Sbjct: 489 NPRTVLDAFLSLEPYDYQQRAGIRLVARKLVIH 487

BLAST of Chy2G047490 vs. ExPASy Swiss-Prot
Match: Q9CA74 (Protein NEN3 OS=Arabidopsis thaliana OX=3702 GN=NEN3 PE=2 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 6.0e-139
Identity = 271/510 (53.14%), Postives = 355/510 (69.61%), Query Frame = 0

Query: 5   DDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLISSLS 64
           D+RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCP++L EL SYSTL++P+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPAP 124
            R +GI+RD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  P P
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           K TIDSL+LL+Q+FG+RAGDMKMA+LA+YFG+G Q HRSLDDVRMNLEV+KYCATVLFLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLE 187

Query: 185 SSLPEVFPENSWVSPNAVTRHRA--------AKSSPQGNSSNNNSHVSSSNIGGNPISLT 244
           SS+P++  + SW SP    R R+         + S   +SS+  +  SSS++    +   
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIVK-- 247

Query: 245 DQQGGTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINMEEHAT 304
                 HPI+SL+T  SE  AS     S    D  ++ T+  ++   TL+TD   EE  T
Sbjct: 248 -----NHPIVSLLTECSESDAS-----SYDIEDPIDITTLIGKLRIGTLQTDA-AEEAKT 307

Query: 305 V--STEASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLC 364
           V    E+ PS D+        FL  ++VSVS I AS VPF+RGS R++L+H D  L L  
Sbjct: 308 VRQQDESPPSPDSDAK--DESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCW 367

Query: 365 NNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAV 424
           ++L+VRFGIS KF D+AGRP+L+ +VD P +LC +L+A D  A  L +DS + S+WRP V
Sbjct: 368 HSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTV 427

Query: 425 TRKSGYFNYPTMRLHIPTAVSGDVANYATEMYQKEAS-GAVQRLIFSKFDAAELDSLIKP 484
            RK G+ NYPT RLHI +  +GD     T++YQKE   G  Q+L  S  +  +L+S + P
Sbjct: 428 IRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLP 487

Query: 485 AAILDAFISLDTYDYQQSAGIRLVAKKLII 504
             ++DAF SL+ Y YQQ AGIRL  KKL+I
Sbjct: 488 GTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 502

BLAST of Chy2G047490 vs. ExPASy Swiss-Prot
Match: F4JJ23 (Protein NEN4 OS=Arabidopsis thaliana OX=3702 GN=NEN4 PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 7.3e-68
Identity = 125/199 (62.81%), Postives = 157/199 (78.89%), Query Frame = 0

Query: 8   SEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLISSLSVRC 67
           +EI FFD+ET VP + GQ F ILEFGAI+VCPKKL ELES++TLI+P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 68  NGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPAPKGT 127
           +GI+R  V ++P+F  +A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  AP P G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 128 IDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSL 187
           IDSL LL+ +FG+RAG+MKMA+LA+YFG+G Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTL 187

Query: 188 PEVFPENSWVSPNAVTRHR 207
           P         S   +TR R
Sbjct: 188 PNHLEGKWHTSSKIMTRSR 206

BLAST of Chy2G047490 vs. ExPASy TrEMBL
Match: A0A0A0LSC3 (Exonuclease domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G009910 PE=4 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.8e-274
Identity = 487/505 (96.44%), Postives = 492/505 (97.43%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MGPT+DRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTL+KPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQG 240
           LFLESSLPEVFPENSWVSPNAVTR RAAKSSPQGNSSNNNSH SSSNIGGNPISL  QQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240

Query: 241 GTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINMEEHATVSTE 300
            THPILSLVT  SEDRAS LAEPSATESDSFNMHT SDQITGATLETDINMEEHATVSTE
Sbjct: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVSTE 300

Query: 301 ASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR 360
           ASPSEDTS SLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR
Sbjct: 301 ASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR 360

Query: 361 FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKSGY 420
           FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRK+GY
Sbjct: 361 FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGY 420

Query: 421 FNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFDAAELDSLIKPAAILDAF 480
           FNYPTMRLHIPTAVSGDVANYATEM+QKEASGAVQRLIFSKFDAAELDSLIKP AILDAF
Sbjct: 421 FNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAF 480

Query: 481 ISLDTYDYQQSAGIRLVAKKLIIHS 506
           ISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 ISLDTYDYQQSAGIRLVAKKLIIRS 505

BLAST of Chy2G047490 vs. ExPASy TrEMBL
Match: A0A1S3BWV4 (protein NEN1 OS=Cucumis melo OX=3656 GN=LOC103494007 PE=4 SV=1)

HSP 1 Score: 953.7 bits (2464), Expect = 3.0e-274
Identity = 487/506 (96.25%), Postives = 494/506 (97.63%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MGPT+DRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTL+KPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQG 240
           LFLESSLPEVFPENSWVSPNAVTR RAAKSSPQGNSSNNNSH SSSNIGGNPISLTDQQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 GTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINME-EHATVST 300
             HPILSLVTCSSED A NLAEPSATESDSFNMHTISDQITGATLETDINME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360
           EASPSEDTS SL TTKFLEPDQVSV FITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV
Sbjct: 301 EASPSEDTSTSLHTTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKSG 420
           RFGISTKFTDYAGRPRLSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRPAVTRK+G
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420

Query: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFDAAELDSLIKPAAILDA 480
           YFNYPTMRLHIPTAVSGDVANYATEMYQK+ASGAVQRLIFSKFDAAELDSLIKP AILDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILDA 480

Query: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 506
           FISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 506

BLAST of Chy2G047490 vs. ExPASy TrEMBL
Match: A0A5A7UTA8 (Protein NEN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G002600 PE=4 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 4.1e-263
Identity = 473/506 (93.48%), Postives = 482/506 (95.26%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MGPT+DRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTL+KPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQRFGRRAGDMK+ T  S         +SLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLH-ANSGDQSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQG 240
           LFLESSLPEVFPENSWVSPNAVTR RAAKSSPQGNSSNNNSH SSSNIGGNPISLTDQQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 GTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINME-EHATVST 300
             HPILSLVTCSSED A NLAEPSATESDSFNMHTISDQITGATLETDINME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360
           EASPSEDTS SL TTKFLEPDQVSV FITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV
Sbjct: 301 EASPSEDTSTSLHTTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKSG 420
           RFGISTKFTDYAGRPRLSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRPAVTRK+G
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420

Query: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFDAAELDSLIKPAAILDA 480
           YFNYPTMRLHIPTAVSGDVANYATEMYQK+ASGAVQRLIFSKFDAAELDSLIKP AILDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILDA 480

Query: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 506
           FISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 505

BLAST of Chy2G047490 vs. ExPASy TrEMBL
Match: A0A6J1E665 (protein NEN1-like OS=Cucurbita moschata OX=3662 GN=LOC111429799 PE=4 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 1.0e-245
Identity = 446/522 (85.44%), Postives = 474/522 (90.80%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KL ELESYSTL++PSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNN--------------NSHVSSS 240
           LFLESSLPEVFPENSWVSPNAVTR RAAKSSPQG ++NN              N+  SSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 241 NIGGNPISLTDQQGGTHPILSLVTCSSEDRASNL-AEPSATESDSFNMHTISDQITGATL 300
            IGG+PISLTDQQG  HPILSLVTCSSED ASNL A+  ATESDSFNM T+SDQI G +L
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDINM-EEHATVSTEASPSEDTSPSLCT-TKFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD +M EEHA+VSTEASP+E+TS S+ + T+FLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 WHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSD 420
            HKDDCLQLLCNNLRVRFGISTKFTDYAGRPRL+FVVDVPP+LC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPAVTRKSGYFNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFD 480
           SGSGSEWRP V RK+GYFNYPTMRLHIPTAVSGD+A YATEMYQKEASG  QRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPAAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 506
           A ELDSLI P  I+DAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Chy2G047490 vs. ExPASy TrEMBL
Match: A0A6J1JJE4 (protein NEN1-like OS=Cucurbita maxima OX=3661 GN=LOC111484975 PE=4 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 1.1e-244
Identity = 446/522 (85.44%), Postives = 470/522 (90.04%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KL ELESYSTL++PSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNN--------------NSHVSSS 240
           LFLESSLPEVFPENSWVSPNAVTR RAAKSSPQG + NN              N+  SSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGINENNSAAKSSPRGINANSNTRSSSS 240

Query: 241 NIGGNPISLTDQQGGTHPILSLVTCSSEDRASNL-AEPSATESDSFNMHTISDQITGATL 300
            IGG+PISLTDQQG  HPILSLVTCSSED ASNL A+  ATESDSFNM T+SDQI G +L
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDINM-EEHATVSTEASPSEDTSPSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD +M EEHA VSTEASP+E+TS SL   T+FLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHAPVSTEASPTENTSTSLSAATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 WHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSD 420
            HKDDCLQLLCNNLRVRFGISTKFTDYAGRPRL+FVVDVPP+LC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPAVTRKSGYFNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFD 480
           SGSGSEWRP V RK+GYFNYPTMRLHIPTAV GD+A YATEMYQKEASG  QRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVCGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPAAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 506
           A ELDSLI P  I+DAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Chy2G047490 vs. NCBI nr
Match: XP_011660176.1 (protein NEN1 [Cucumis sativus] >KGN63687.1 hypothetical protein Csa_013241 [Cucumis sativus])

HSP 1 Score: 951 bits (2457), Expect = 0.0
Identity = 487/505 (96.44%), Postives = 492/505 (97.43%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MGPT+DRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTL+KPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQG 240
           LFLESSLPEVFPENSWVSPNAVTR RAAKSSPQGNSSNNNSH SSSNIGGNPISL  QQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLPVQQG 240

Query: 241 GTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINMEEHATVSTE 300
            THPILSLVT  SEDRAS LAEPSATESDSFNMHT SDQITGATLETDINMEEHATVSTE
Sbjct: 241 ETHPILSLVTICSEDRASILAEPSATESDSFNMHTTSDQITGATLETDINMEEHATVSTE 300

Query: 301 ASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR 360
           ASPSEDTS SLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR
Sbjct: 301 ASPSEDTSTSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRVR 360

Query: 361 FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKSGY 420
           FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRK+GY
Sbjct: 361 FGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNGY 420

Query: 421 FNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFDAAELDSLIKPAAILDAF 480
           FNYPTMRLHIPTAVSGDVANYATEM+QKEASGAVQRLIFSKFDAAELDSLIKP AILDAF
Sbjct: 421 FNYPTMRLHIPTAVSGDVANYATEMHQKEASGAVQRLIFSKFDAAELDSLIKPGAILDAF 480

Query: 481 ISLDTYDYQQSAGIRLVAKKLIIHS 505
           ISLDTYDYQQSAGIRLVAKKLII S
Sbjct: 481 ISLDTYDYQQSAGIRLVAKKLIIRS 505

BLAST of Chy2G047490 vs. NCBI nr
Match: XP_008453220.1 (PREDICTED: protein NEN1 [Cucumis melo])

HSP 1 Score: 950 bits (2455), Expect = 0.0
Identity = 487/506 (96.25%), Postives = 494/506 (97.63%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MGPT+DRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTL+KPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQG 240
           LFLESSLPEVFPENSWVSPNAVTR RAAKSSPQGNSSNNNSH SSSNIGGNPISLTDQQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 GTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINME-EHATVST 300
             HPILSLVTCSSED A NLAEPSATESDSFNMHTISDQITGATLETDINME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360
           EASPSEDTS SL TTKFLEPDQVSV FITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV
Sbjct: 301 EASPSEDTSTSLHTTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKSG 420
           RFGISTKFTDYAGRPRLSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRPAVTRK+G
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420

Query: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFDAAELDSLIKPAAILDA 480
           YFNYPTMRLHIPTAVSGDVANYATEMYQK+ASGAVQRLIFSKFDAAELDSLIKP AILDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILDA 480

Query: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 505
           FISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 506

BLAST of Chy2G047490 vs. NCBI nr
Match: KAA0057957.1 (protein NEN1 [Cucumis melo var. makuwa])

HSP 1 Score: 913 bits (2359), Expect = 0.0
Identity = 473/506 (93.48%), Postives = 482/506 (95.26%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MGPT+DRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTL+KPSDLSLI
Sbjct: 1   MGPTEDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLVKPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFA+IGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFADIGVP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQRFGRRAGDMK+ T  S         +SLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKVMTEFSLLH-ANSGDQSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQG 240
           LFLESSLPEVFPENSWVSPNAVTR RAAKSSPQGNSSNNNSH SSSNIGGNPISLTDQQG
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGNSSNNNSHASSSNIGGNPISLTDQQG 240

Query: 241 GTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINME-EHATVST 300
             HPILSLVTCSSED A NLAEPSATESDSFNMHTISDQITGATLETDINME EHATVST
Sbjct: 241 EAHPILSLVTCSSEDGALNLAEPSATESDSFNMHTISDQITGATLETDINMEGEHATVST 300

Query: 301 EASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360
           EASPSEDTS SL TTKFLEPDQVSV FITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV
Sbjct: 301 EASPSEDTSTSLHTTKFLEPDQVSVLFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLRV 360

Query: 361 RFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKSG 420
           RFGISTKFTDYAGRPRLSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRPAVTRK+G
Sbjct: 361 RFGISTKFTDYAGRPRLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKNG 420

Query: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFDAAELDSLIKPAAILDA 480
           YFNYPTMRLHIPTAVSGDVANYATEMYQK+ASGAVQRLIFSKFDAAELDSLIKP AILDA
Sbjct: 421 YFNYPTMRLHIPTAVSGDVANYATEMYQKDASGAVQRLIFSKFDAAELDSLIKPGAILDA 480

Query: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 505
           FISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 FISLDTYDYQQSAGIRLVAKKLIIHS 505

BLAST of Chy2G047490 vs. NCBI nr
Match: XP_038880150.1 (protein NEN1-like [Benincasa hispida])

HSP 1 Score: 892 bits (2306), Expect = 0.0
Identity = 464/507 (91.52%), Postives = 481/507 (94.87%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MG T+DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KLVELESYSTL++PSDLSLI
Sbjct: 1   MGSTEDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLVELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP
Sbjct: 61  SSLSVRCNGITRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APEPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQG 240
           LFLESSLPEVF ENSWVSPNAVTR R AKSSPQGNSSNN++H SSS IGGNPISL DQ G
Sbjct: 181 LFLESSLPEVFRENSWVSPNAVTRRRTAKSSPQGNSSNNDTHSSSSRIGGNPISLLDQ-G 240

Query: 241 GTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINMEE-HATVST 300
            THPILSLVTCSSED ASNLAE  ATESDSFNM T+SDQITG +LETDI+MEE HATVST
Sbjct: 241 ETHPILSLVTCSSEDGASNLAESRATESDSFNMRTLSDQITGESLETDISMEEEHATVST 300

Query: 301 EASPSEDTSPSL-CTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLCNNLR 360
           EASPSEDTS SL  TT+FLEPDQVSVSFITASFVPFFRGSQRIQL HKDDCLQLLCNNLR
Sbjct: 301 EASPSEDTSTSLSATTEFLEPDQVSVSFITASFVPFFRGSQRIQLSHKDDCLQLLCNNLR 360

Query: 361 VRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKS 420
           VRFGISTKFTDYAGRP+LSFVVDVPP+LCTVLEASDGVAQRLFSDSGSGSEWRP V RK+
Sbjct: 361 VRFGISTKFTDYAGRPKLSFVVDVPPSLCTVLEASDGVAQRLFSDSGSGSEWRPVVMRKN 420

Query: 421 GYFNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFDAAELDSLIKPAAILD 480
            YFNYPTMRLHIPTAVSGDVA YATEMYQKE+SGA QRLIFSKFDAAELDSLIKP AILD
Sbjct: 421 DYFNYPTMRLHIPTAVSGDVAIYATEMYQKESSGAAQRLIFSKFDAAELDSLIKPGAILD 480

Query: 481 AFISLDTYDYQQSAGIRLVAKKLIIHS 505
           AFISLDTYDYQQSAGIRLVAK+LIIHS
Sbjct: 481 AFISLDTYDYQQSAGIRLVAKRLIIHS 506

BLAST of Chy2G047490 vs. NCBI nr
Match: XP_022921575.1 (protein NEN1-like [Cucurbita moschata] >KAG6589518.1 Protein NEN2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 855 bits (2209), Expect = 4.60e-310
Identity = 446/522 (85.44%), Postives = 474/522 (90.80%), Query Frame = 0

Query: 1   MGPTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLI 60
           MGPT DRSEIAFFDVETTVPTRQGQ+FSILEFGAILVCP+KL ELESYSTL++PSDLSLI
Sbjct: 1   MGPTGDRSEIAFFDVETTVPTRQGQRFSILEFGAILVCPRKLDELESYSTLVRPSDLSLI 60

Query: 61  SSLSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVP 120
           SSLSVRCNGI+RDAVIS+PTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIG+P
Sbjct: 61  SSLSVRCNGITRDAVISAPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGMP 120

Query: 121 APAPKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180
           AP PKGTIDSLALLTQ+FGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV
Sbjct: 121 APQPKGTIDSLALLTQKFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATV 180

Query: 181 LFLESSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNN--------------NSHVSSS 240
           LFLESSLPEVFPENSWVSPNAVTR RAAKSSPQG ++NN              N+  SSS
Sbjct: 181 LFLESSLPEVFPENSWVSPNAVTRRRAAKSSPQGTNANNSAAKASPQGINANSNTRSSSS 240

Query: 241 NIGGNPISLTDQQGGTHPILSLVTCSSEDRASNL-AEPSATESDSFNMHTISDQITGATL 300
            IGG+PISLTDQQG  HPILSLVTCSSED ASNL A+  ATESDSFNM T+SDQI G +L
Sbjct: 241 RIGGSPISLTDQQGEAHPILSLVTCSSEDGASNLVADSDATESDSFNMGTLSDQIAGESL 300

Query: 301 ETDINMEE-HATVSTEASPSEDTSPSLCT-TKFLEPDQVSVSFITASFVPFFRGSQRIQL 360
           ETD +MEE HA+VSTEASP+E+TS S+ + T+FLEPDQVSVS ITASFVPFFRGSQRIQL
Sbjct: 301 ETDTSMEEEHASVSTEASPTENTSTSISSATEFLEPDQVSVSSITASFVPFFRGSQRIQL 360

Query: 361 WHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSD 420
            HKDDCLQLLCNNLRVRFGISTKFTDYAGRPRL+FVVDVPP+LC VLEASDGVAQRLFS+
Sbjct: 361 SHKDDCLQLLCNNLRVRFGISTKFTDYAGRPRLNFVVDVPPSLCRVLEASDGVAQRLFSE 420

Query: 421 SGSGSEWRPAVTRKSGYFNYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFD 480
           SGSGSEWRP V RK+GYFNYPTMRLHIPTAVSGD+A YATEMYQKEASG  QRLIFSKFD
Sbjct: 421 SGSGSEWRPVVMRKNGYFNYPTMRLHIPTAVSGDMAIYATEMYQKEASGGAQRLIFSKFD 480

Query: 481 AAELDSLIKPAAILDAFISLDTYDYQQSAGIRLVAKKLIIHS 505
           A ELDSLI P  I+DAFISLDTYDYQQSAGIRLVAKKLIIHS
Sbjct: 481 AVELDSLINPGGIIDAFISLDTYDYQQSAGIRLVAKKLIIHS 522

BLAST of Chy2G047490 vs. TAIR 10
Match: AT5G07710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 526.6 bits (1355), Expect = 2.3e-149
Identity = 285/504 (56.55%), Postives = 353/504 (70.04%), Query Frame = 0

Query: 5   DDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLISSLS 64
           +DRSEIAFFDVETTVP R GQ+F+ILEFG+ILVCPKKL EL SY+TL++P+DLSLISSLS
Sbjct: 4   EDRSEIAFFDVETTVPKR-GQRFAILEFGSILVCPKKLTELRSYTTLVQPADLSLISSLS 63

Query: 65  VRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPAP 124
           VRCNGI RD V+ +P FA IAD VYDILHGRIWAGHNILRFDCARIREAFAEIG   P P
Sbjct: 64  VRCNGIKRDDVVLAPLFADIADTVYDILHGRIWAGHNILRFDCARIREAFAEIGRQPPEP 123

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           KG IDSL LLTQ+FGRRAGDMKMATLA YFG+G QTHRSLDDVRMNLEVLKYCATVLFLE
Sbjct: 124 KGAIDSLGLLTQKFGRRAGDMKMATLARYFGLGNQTHRSLDDVRMNLEVLKYCATVLFLE 183

Query: 185 SSLPEVFPENSWVSPNAVTRHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQGGTHP 244
           SSLP    +NS VSP  ++  R   +S +GN                             
Sbjct: 184 SSLPYAHVDNS-VSPETISSRRRIDASREGN----------------------------- 243

Query: 245 ILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGAT-LETDINMEEHATVSTEASP 304
                T ++  R  +++E SA + D FNM  + +++     +++DI MEE     ++   
Sbjct: 244 -----TVTTSVRLPSISENSAAQPDPFNMSVLRNEMASDNHIQSDILMEEEQIEPSDVVA 303

Query: 305 SEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQ--RIQLWHKDDCLQLLCNNLRVRF 364
           SE+TS       FL PD +S+S I A   PF+ GSQ  +++L H D  LQL C+ L++RF
Sbjct: 304 SENTSDH---EGFLTPDAMSLSNIKAMLFPFYPGSQMMKLKLLHGDSPLQLYCSYLKIRF 363

Query: 365 GISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAVTRKSGYF 424
           G++ KF D  GR RL+FVVD+ P+L ++LEA D  AQ+L  DSGS SEW P V    G+ 
Sbjct: 364 GVNGKFLDNTGRRRLNFVVDLNPSLYSILEACDSNAQKLSVDSGSTSEWNPVVNPMKGFV 423

Query: 425 NYPTMRLHIPTAVSGDVANYATEMYQKEASGAVQRLIFSKFDAAELDSLIKPAAILDAFI 484
           NYP  R+HI T ++GD A YATE++Q+E+SGA Q+LIFS  +  EL+SL+   +++DAF+
Sbjct: 424 NYPNARIHIATEINGDEARYATEIHQRESSGATQKLIFSNPNNEELESLLTTGSVVDAFL 468

Query: 485 SLDTYDYQQSAGIRLVAKKLIIHS 506
           SL+ YDYQQ AGIRLVAKKL+I S
Sbjct: 484 SLEPYDYQQKAGIRLVAKKLVIQS 468

BLAST of Chy2G047490 vs. TAIR 10
Match: AT5G61390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 519.6 bits (1337), Expect = 2.8e-147
Identity = 287/513 (55.95%), Postives = 353/513 (68.81%), Query Frame = 0

Query: 3   PTDDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLISS 62
           P +DRSEIAFFDVETT+P R GQ ++ILEFG+ILVCPKKLVEL++YS L++P++L+LI+ 
Sbjct: 9   PAEDRSEIAFFDVETTIPFRVGQGYAILEFGSILVCPKKLVELKNYSVLVRPANLNLITP 68

Query: 63  LSVRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAP 122
            SV+CNGI R+ V S+ TFA IAD VYDILHGRIWAGHNIL+FDC RIREAFAEIG   P
Sbjct: 69  RSVKCNGIKREDVESALTFADIADTVYDILHGRIWAGHNILKFDCPRIREAFAEIGRDPP 128

Query: 123 APKGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLF 182
            PKGTIDSLALLTQRFGRRAGDMKMATLA+YFG+G QTHRSLDDVRMN EVLKYCATVLF
Sbjct: 129 EPKGTIDSLALLTQRFGRRAGDMKMATLATYFGLGNQTHRSLDDVRMNFEVLKYCATVLF 188

Query: 183 LESSLPEVFPENSWVSPNAVT---RHRAAKSSPQGNSSNNNSHVSSSNIGGNPISLTDQQ 242
           LESSLP+   ENS  +    T   R R  K SP                     S TDQQ
Sbjct: 189 LESSLPDELIENSVTTTTPETSSRRRRTIKKSPLQ-------------------SPTDQQ 248

Query: 243 GG----THPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINMEEHA 302
            G    T PILS V+ +              ++D F+M T+ ++I    L++D+ MEE  
Sbjct: 249 TGENMTTIPILSFVSSAE------------AQTDPFDMSTLRNEIAPEVLQSDVPMEEEQ 308

Query: 303 TVSTEASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQ--RIQLWHKDDCLQLL 362
              +E   SE T        F+E D++SVS I A+ VP + GSQ  ++QL+  D  LQL 
Sbjct: 309 NQQSETVASEGTGDQ---EGFMELDKISVSSIRATHVPLYDGSQTMKLQLFLGDRPLQLH 368

Query: 363 CNNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPA 422
           C  L+VRFGI+ KF D AGR RL+FV+D+ P+LC VL+  D  AQ +  DSGSGS+W P 
Sbjct: 369 CPRLKVRFGINGKFMDKAGRRRLNFVIDLYPSLCNVLQECDSAAQTISVDSGSGSDWNPL 428

Query: 423 VTRKSGYFNYPTMRLHIPTAVSGDVANYATEMYQKEASG--AVQRLIFSKFDAAELDSLI 482
           V    G+ N PT R+HIPT ++GD+  YA E++QKE SG  A Q+LI S   A E++SL+
Sbjct: 429 VIPMKGFLNCPTARIHIPTELNGDIDRYAAEIHQKEFSGATATQKLISSNPKAEEIESLL 487

Query: 483 KPAAILDAFISLDTYDYQQSAGIRLVAKKLIIH 505
            P  +LDAF+SL+ YDYQQ AGIRLVA+KL+IH
Sbjct: 489 NPRTVLDAFLSLEPYDYQQRAGIRLVARKLVIH 487

BLAST of Chy2G047490 vs. TAIR 10
Match: AT1G74390.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 495.7 bits (1275), Expect = 4.3e-140
Identity = 271/510 (53.14%), Postives = 355/510 (69.61%), Query Frame = 0

Query: 5   DDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLISSLS 64
           D+RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCP++L EL SYSTL++P+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPAP 124
            R +GI+RD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  P P
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           K TIDSL+LL+Q+FG+RAGDMKMA+LA+YFG+G Q HRSLDDVRMNLEV+KYCATVLFLE
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLEVVKYCATVLFLE 187

Query: 185 SSLPEVFPENSWVSPNAVTRHRA--------AKSSPQGNSSNNNSHVSSSNIGGNPISLT 244
           SS+P++  + SW SP    R R+         + S   +SS+  +  SSS++    +   
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIVK-- 247

Query: 245 DQQGGTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINMEEHAT 304
                 HPI+SL+T  SE  AS     S    D  ++ T+  ++   TL+TD   EE  T
Sbjct: 248 -----NHPIVSLLTECSESDAS-----SYDIEDPIDITTLIGKLRIGTLQTDA-AEEAKT 307

Query: 305 V--STEASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLC 364
           V    E+ PS D+        FL  ++VSVS I AS VPF+RGS R++L+H D  L L  
Sbjct: 308 VRQQDESPPSPDSDAK--DESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCW 367

Query: 365 NNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAV 424
           ++L+VRFGIS KF D+AGRP+L+ +VD P +LC +L+A D  A  L +DS + S+WRP V
Sbjct: 368 HSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTV 427

Query: 425 TRKSGYFNYPTMRLHIPTAVSGDVANYATEMYQKEAS-GAVQRLIFSKFDAAELDSLIKP 484
            RK G+ NYPT RLHI +  +GD     T++YQKE   G  Q+L  S  +  +L+S + P
Sbjct: 428 IRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLP 487

Query: 485 AAILDAFISLDTYDYQQSAGIRLVAKKLII 504
             ++DAF SL+ Y YQQ AGIRL  KKL+I
Sbjct: 488 GTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 502

BLAST of Chy2G047490 vs. TAIR 10
Match: AT1G74390.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 466.8 bits (1200), Expect = 2.1e-131
Identity = 261/510 (51.18%), Postives = 344/510 (67.45%), Query Frame = 0

Query: 5   DDRSEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLISSLS 64
           D+RSEIAFFD+ET VPT+ G+ F+ILEFGAILVCP++L EL SYSTL++P+DLSLIS+L+
Sbjct: 8   DERSEIAFFDLETAVPTKSGEPFAILEFGAILVCPRRLEELYSYSTLVRPTDLSLISTLT 67

Query: 65  VRCNGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPAP 124
            R +GI+RD V+S+ TF++IAD+VYDILHGRIWAGHNI+RFDC RIREAFAEIG+  P P
Sbjct: 68  KRRSGITRDGVLSAHTFSEIADKVYDILHGRIWAGHNIIRFDCVRIREAFAEIGLSPPEP 127

Query: 125 KGTIDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLE 184
           K TIDSL+LL+Q+FG+RAGDMKMA+LA+YFG+G Q HRSLDDVRMNLE           E
Sbjct: 128 KATIDSLSLLSQKFGKRAGDMKMASLATYFGLGDQAHRSLDDVRMNLE-----------E 187

Query: 185 SSLPEVFPENSWVSPNAVTRHRA--------AKSSPQGNSSNNNSHVSSSNIGGNPISLT 244
           SS+P++  + SW SP    R R+         + S   +SS+  +  SSS++    +   
Sbjct: 188 SSVPDILTDMSWFSPRKSPRTRSNGKLVANGVRESSTSSSSSPKTDPSSSSVDATIVK-- 247

Query: 245 DQQGGTHPILSLVTCSSEDRASNLAEPSATESDSFNMHTISDQITGATLETDINMEEHAT 304
                 HPI+SL+T  SE  AS     S    D  ++ T+  ++   TL+TD   EE  T
Sbjct: 248 -----NHPIVSLLTECSESDAS-----SYDIEDPIDITTLIGKLRIGTLQTDA-AEEAKT 307

Query: 305 V--STEASPSEDTSPSLCTTKFLEPDQVSVSFITASFVPFFRGSQRIQLWHKDDCLQLLC 364
           V    E+ PS D+        FL  ++VSVS I AS VPF+RGS R++L+H D  L L  
Sbjct: 308 VRQQDESPPSPDSDAK--DESFLGVNEVSVSSIRASLVPFYRGSLRMKLFHNDTPLHLCW 367

Query: 365 NNLRVRFGISTKFTDYAGRPRLSFVVDVPPNLCTVLEASDGVAQRLFSDSGSGSEWRPAV 424
           ++L+VRFGIS KF D+AGRP+L+ +VD P +LC +L+A D  A  L +DS + S+WRP V
Sbjct: 368 HSLKVRFGISRKFVDHAGRPKLNIIVDAPLDLCKILDAVDAAAHNLPTDSSTNSDWRPTV 427

Query: 425 TRKSGYFNYPTMRLHIPTAVSGDVANYATEMYQKEAS-GAVQRLIFSKFDAAELDSLIKP 484
            RK G+ NYPT RLHI +  +GD     T++YQKE   G  Q+L  S  +  +L+S + P
Sbjct: 428 IRKEGFANYPTARLHISSESNGDDTLCGTQVYQKEEPLGTNQKLDVSSDNLEKLESALLP 487

Query: 485 AAILDAFISLDTYDYQQSAGIRLVAKKLII 504
             ++DAF SL+ Y YQQ AGIRL  KKL+I
Sbjct: 488 GTLVDAFFSLEPYSYQQMAGIRLAVKKLVI 491

BLAST of Chy2G047490 vs. TAIR 10
Match: AT4G39810.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 259.6 bits (662), Expect = 5.2e-69
Identity = 125/199 (62.81%), Postives = 157/199 (78.89%), Query Frame = 0

Query: 8   SEIAFFDVETTVPTRQGQKFSILEFGAILVCPKKLVELESYSTLIKPSDLSLISSLSVRC 67
           +EI FFD+ET VP + GQ F ILEFGAI+VCPKKL ELES++TLI+P DLS++S  S R 
Sbjct: 8   NEIVFFDLETNVPNKAGQHFHILEFGAIIVCPKKLEELESFTTLIQPKDLSVVSIRSSRS 67

Query: 68  NGISRDAVISSPTFAQIADRVYDILHGRIWAGHNILRFDCARIREAFAEIGVPAPAPKGT 127
           +GI+R  V ++P+F  +A++++ +L+GRIWAGHNI RFDC RI+EAFAEIG  AP P G 
Sbjct: 68  DGITRAKVTNAPSFEDVAEKIHGLLNGRIWAGHNIRRFDCVRIKEAFAEIGKAAPEPSGI 127

Query: 128 IDSLALLTQRFGRRAGDMKMATLASYFGIGQQTHRSLDDVRMNLEVLKYCATVLFLESSL 187
           IDSL LL+ +FG+RAG+MKMA+LA+YFG+G Q HRSLDDVRMNLEVLK+CATVLFLES+L
Sbjct: 128 IDSLGLLSDKFGKRAGNMKMASLAAYFGLGVQKHRSLDDVRMNLEVLKHCATVLFLESTL 187

Query: 188 PEVFPENSWVSPNAVTRHR 207
           P         S   +TR R
Sbjct: 188 PNHLEGKWHTSSKIMTRSR 206