Chy2G029520 (gene) Cucumber (hystrix) v1

Overview
NameChy2G029520
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptioncytochrome P450 CYP736A12-like
LocationchrH02: 4160684 .. 4162413 (+)
RNA-Seq ExpressionChy2G029520
SyntenyChy2G029520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTTGGATTATTTTGACCACAATTACACTCATCTTCCTTCTCCCTTGCCTCTTTCAACAATGGCTTCTCAAGACCAAGCCCTTATGCAACAAACTCCCTCCTGGCCCAAAAGGGTTTCCAATTATTGGAAGCCTTCATCTATTAGGCAAACTCATTCACAAAGACCTTCATTACTTATCCCAAATATACGGTCCCATAATGCACATCCAATTAGGTCTTCTCCCCGCCATTATCGTCTCCTCCGCTAGAGCCACCGAGCTCTTCCTTAAAACTCACGACCTTCACTTTGCGAGACGCCCTCTTACCATCACTTCCAACCATATTTCTTACGCTAGAAAGGGCATAACTTTTGCGCAATATGGTCCTTATTGGCGCAATATTCGCAAAATGTGCACTCTTGAATTGCTTAGCTCCTTAAAAATCAACTATTTTTCCTCAATGAGAAAGCAAGAGGTTGGTTCGTTGATTGAGGGTCTTGAAGTGGCTGCTACAGATGGCGTGGCTGTGGATCTTACTTCTACAATTTCTTGTGTTATTGTAGATATGATATGTGTAATGGTGTTGGGGAGAAAATATGAAGACAGTGAGTTGGATGAAAAGGGGTTTAAGGGTTTGATACGAGAGGCTACACAATTAGCGGCAGCTCCTAACTTGGGAGATTTTATTCCTTTAATTGCAAGGTTTGATGTTCAAGGATTTGGGGGTCGTGCAAAAGCTGTTGGGAAAATATTTGATGTGTTTTTAGAGAGGATTGTTGAAGAACATGTTGTGTTCGAGAGGGATAATAAAGACAAGGATTTTGTGGATGTTATGTTGGATTTGATGGGATCTCGAGAGTACCAAATTGATCGATCAAATATCAAAGCTATAATCCTGGTAAGTGAATTGATTGATTGAGGGTGTGTGTTGGATTTCTTTTTTCTTTAGTGTTTAATTTGAAAGAGCTTTGTTTTTAAATACAGCAAAAAAAATTATTGTTTATTAGTCAAAACATTTATCATGGATAGATCTCTATAATAGTGTCTATCAAATTTTGCTAAACTCTCATTTTAAATTATTTTAAAGAATGTAACTAAACCTAAGAATATTTCTTAATTTGTTTGCAGGATTTCCTTATTGCAGCCGTGGATAGTACAACAACAACAATCAATTGGATGTTATCAGAACTTATTAAGCATCCACACATAATGAAAAAGTTGCAAGAAGAATTAGAAAAAGTGGTGGGATTGAATAGAATGGTGGAAGAATCAGACTTAAGTAACTTGAAATACTTAGAGATGGTAATAAAAGAGAGTTTGAGAATGCATCCACCAGTTCCATTAATTCCAAGAGAATGCATACAAGATTGCAATATAAATGGGTATCATATCCCTGTGAAATCACGAATAGTAATAAATGCATGGGCCATTGGAAGAGATCCAAATACTTGGGTTGATCCGCACAAGTTTGATCCAGAGAGATTTTTGGAAAGTGAAATGGATGTGAAAGGAAGAGATTTTGAACTAATTCCATTTGGATCGGGTCGAAGAGGCTGTGTTGGAATACAATTAGCTTTGGTTGTGGTTCGTCATGTAGTGGCTCAATTAGTACACTGTTTTGATTTGAAGCTTCCCAATGGTATGTCACCCCTTGAATTGGATATGACAGAAATTTTGGGGTTGACCTGTTCTAGAGCCCAAAATCTTACGGTGATTCCCATTTTTCGTCTTTGTCGTAATTATTTATAA

mRNA sequence

ATGACTTGGATTATTTTGACCACAATTACACTCATCTTCCTTCTCCCTTGCCTCTTTCAACAATGGCTTCTCAAGACCAAGCCCTTATGCAACAAACTCCCTCCTGGCCCAAAAGGGTTTCCAATTATTGGAAGCCTTCATCTATTAGGCAAACTCATTCACAAAGACCTTCATTACTTATCCCAAATATACGGTCCCATAATGCACATCCAATTAGGTCTTCTCCCCGCCATTATCGTCTCCTCCGCTAGAGCCACCGAGCTCTTCCTTAAAACTCACGACCTTCACTTTGCGAGACGCCCTCTTACCATCACTTCCAACCATATTTCTTACGCTAGAAAGGGCATAACTTTTGCGCAATATGGTCCTTATTGGCGCAATATTCGCAAAATGTGCACTCTTGAATTGCTTAGCTCCTTAAAAATCAACTATTTTTCCTCAATGAGAAAGCAAGAGGTTGGTTCGTTGATTGAGGGTCTTGAAGTGGCTGCTACAGATGGCGTGGCTGTGGATCTTACTTCTACAATTTCTTGTGTTATTGTAGATATGATATGTGTAATGGTGTTGGGGAGAAAATATGAAGACAGTGAGTTGGATGAAAAGGGGTTTAAGGGTTTGATACGAGAGGCTACACAATTAGCGGCAGCTCCTAACTTGGGAGATTTTATTCCTTTAATTGCAAGGTTTGATGTTCAAGGATTTGGGGGTCGTGCAAAAGCTGTTGGGAAAATATTTGATGTGTTTTTAGAGAGGATTGTTGAAGAACATGTTGTGTTCGAGAGGGATAATAAAGACAAGGATTTTGTGGATGTTATGTTGGATTTGATGGGATCTCGAGAGTACCAAATTGATCGATCAAATATCAAAGCTATAATCCTGGATTTCCTTATTGCAGCCGTGGATAGTACAACAACAACAATCAATTGGATGTTATCAGAACTTATTAAGCATCCACACATAATGAAAAAGTTGCAAGAAGAATTAGAAAAAGTGGTGGGATTGAATAGAATGGTGGAAGAATCAGACTTAAGTAACTTGAAATACTTAGAGATGGTAATAAAAGAGAGTTTGAGAATGCATCCACCAGTTCCATTAATTCCAAGAGAATGCATACAAGATTGCAATATAAATGGGTATCATATCCCTGTGAAATCACGAATAGTAATAAATGCATGGGCCATTGGAAGAGATCCAAATACTTGGGTTGATCCGCACAAGTTTGATCCAGAGAGATTTTTGGAAAGTGAAATGGATGTGAAAGGAAGAGATTTTGAACTAATTCCATTTGGATCGGGTCGAAGAGGCTGTGTTGGAATACAATTAGCTTTGGTTGTGGTTCGTCATGTAGTGGCTCAATTAGTACACTGTTTTGATTTGAAGCTTCCCAATGGTATGTCACCCCTTGAATTGGATATGACAGAAATTTTGGGGTTGACCTGTTCTAGAGCCCAAAATCTTACGGTGATTCCCATTTTTCGTCTTTGTCGTAATTATTTATAA

Coding sequence (CDS)

ATGACTTGGATTATTTTGACCACAATTACACTCATCTTCCTTCTCCCTTGCCTCTTTCAACAATGGCTTCTCAAGACCAAGCCCTTATGCAACAAACTCCCTCCTGGCCCAAAAGGGTTTCCAATTATTGGAAGCCTTCATCTATTAGGCAAACTCATTCACAAAGACCTTCATTACTTATCCCAAATATACGGTCCCATAATGCACATCCAATTAGGTCTTCTCCCCGCCATTATCGTCTCCTCCGCTAGAGCCACCGAGCTCTTCCTTAAAACTCACGACCTTCACTTTGCGAGACGCCCTCTTACCATCACTTCCAACCATATTTCTTACGCTAGAAAGGGCATAACTTTTGCGCAATATGGTCCTTATTGGCGCAATATTCGCAAAATGTGCACTCTTGAATTGCTTAGCTCCTTAAAAATCAACTATTTTTCCTCAATGAGAAAGCAAGAGGTTGGTTCGTTGATTGAGGGTCTTGAAGTGGCTGCTACAGATGGCGTGGCTGTGGATCTTACTTCTACAATTTCTTGTGTTATTGTAGATATGATATGTGTAATGGTGTTGGGGAGAAAATATGAAGACAGTGAGTTGGATGAAAAGGGGTTTAAGGGTTTGATACGAGAGGCTACACAATTAGCGGCAGCTCCTAACTTGGGAGATTTTATTCCTTTAATTGCAAGGTTTGATGTTCAAGGATTTGGGGGTCGTGCAAAAGCTGTTGGGAAAATATTTGATGTGTTTTTAGAGAGGATTGTTGAAGAACATGTTGTGTTCGAGAGGGATAATAAAGACAAGGATTTTGTGGATGTTATGTTGGATTTGATGGGATCTCGAGAGTACCAAATTGATCGATCAAATATCAAAGCTATAATCCTGGATTTCCTTATTGCAGCCGTGGATAGTACAACAACAACAATCAATTGGATGTTATCAGAACTTATTAAGCATCCACACATAATGAAAAAGTTGCAAGAAGAATTAGAAAAAGTGGTGGGATTGAATAGAATGGTGGAAGAATCAGACTTAAGTAACTTGAAATACTTAGAGATGGTAATAAAAGAGAGTTTGAGAATGCATCCACCAGTTCCATTAATTCCAAGAGAATGCATACAAGATTGCAATATAAATGGGTATCATATCCCTGTGAAATCACGAATAGTAATAAATGCATGGGCCATTGGAAGAGATCCAAATACTTGGGTTGATCCGCACAAGTTTGATCCAGAGAGATTTTTGGAAAGTGAAATGGATGTGAAAGGAAGAGATTTTGAACTAATTCCATTTGGATCGGGTCGAAGAGGCTGTGTTGGAATACAATTAGCTTTGGTTGTGGTTCGTCATGTAGTGGCTCAATTAGTACACTGTTTTGATTTGAAGCTTCCCAATGGTATGTCACCCCTTGAATTGGATATGACAGAAATTTTGGGGTTGACCTGTTCTAGAGCCCAAAATCTTACGGTGATTCCCATTTTTCGTCTTTGTCGTAATTATTTATAA

Protein sequence

MTWIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVDMICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSREYQIDRSNIKAIILDFLIAAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMHPPVPLIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVKGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTCSRAQNLTVIPIFRLCRNYL*
Homology
BLAST of Chy2G029520 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 8.9e-159
Identity = 289/498 (58.03%), Postives = 363/498 (72.89%), Query Frame = 0

Query: 1   MTWIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           M W I  TI L+ L+  L   W  K K    +LPPGP+GFPI GSLHLLG+  +KDLH L
Sbjct: 1   MVW-IWATIGLLALVHILQAWWKNKKK----RLPPGPRGFPIFGSLHLLGEFPNKDLHRL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQ 120
           ++ YG IM+++LGL+P I++SS  A ELFLKTHDL FA RP    S HIS+ +K + F++
Sbjct: 61  ARKYGDIMYMRLGLMPTIVISSPEAAELFLKTHDLVFASRPPHEGSKHISFGQKNLIFSE 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATD-GVAVDLTSTISCV 180
           YG YWR+ RKMCT+ELLS+ KIN F SMR++EV   +E +  AA + GVAVDL+  +S +
Sbjct: 121 YGAYWRDTRKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSL 180

Query: 181 IVDMICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAK 240
            VDM C MVLG+KY D E DE+GFK ++REA QLA+APNLGD+I  IA  D+QGF  R K
Sbjct: 181 SVDMSCRMVLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMK 240

Query: 241 AVGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSR--EYQIDRSNIKAIILDFLI 300
           +V K FD   E+I+EEH+      +  DFVDVM+  MGS   EY+I+R +IKAI+LD L+
Sbjct: 241 SVNKAFDNLFEKIIEEHLQPNDGERTMDFVDVMVGFMGSEESEYRIERPHIKAIMLDMLV 300

Query: 301 AAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESL 360
           A++D++ TTI W LSEL++HP  MKK+Q+ELE VVGL++MVEESDL  L YL MV+KE+ 
Sbjct: 301 ASMDTSATTIEWALSELMRHPKAMKKVQKELENVVGLDKMVEESDLEKLDYLNMVVKETF 360

Query: 361 RMHPPVP-LIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESE 420
           R+HP  P LIP   I+DC +NGYHIP KSR++IN WAIGRDPN W D  KF PERF  S 
Sbjct: 361 RLHPVAPLLIPHASIEDCTVNGYHIPKKSRVLINVWAIGRDPNAWTDAEKFIPERFEGSS 420

Query: 421 MDVKGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEIL 480
           +DV+G  F+LIPFGSGRR C GIQL L VV+ V+AQLVHCFD +LPN M P ELDMTE  
Sbjct: 421 VDVRGNHFQLIPFGSGRRRCPGIQLGLTVVQLVLAQLVHCFDWELPNNMLPEELDMTEEF 480

Query: 481 GLTCSRAQNLTVIPIFRL 495
           GLT  RA++L  IP +RL
Sbjct: 481 GLTVPRAKHLLAIPSYRL 493

BLAST of Chy2G029520 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.8e-127
Identity = 241/497 (48.49%), Postives = 332/497 (66.80%), Query Frame = 0

Query: 11  LIFLLPCLFQQWLLK-TKPL--CNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPI 70
           L+F+L      W+L    PL   +KLPPGP+G PIIGSLH LG L H+ L  L++ YGPI
Sbjct: 8   LLFVLLGALSWWILPIISPLKRHHKLPPGPRGLPIIGSLHTLGALPHRTLQTLAKKYGPI 67

Query: 71  MHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYGPYWRN 130
           M ++LG +P I+VSS +A ELFLKTHD  FA RP    + ++SY   G++F  YGP+WRN
Sbjct: 68  MSMRLGSVPTIVVSSPQAAELFLKTHDNIFASRPKLQAAEYMSYGTMGMSFTAYGPHWRN 127

Query: 131 IRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVDMICVM 190
           IRK   LELL+  KIN F  MR++E+G++++ ++ A+     VDL++ ++ +I +M   +
Sbjct: 128 IRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRL 187

Query: 191 VLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVGKIFDV 250
           +LGR  +    D    KG++ EA  LA   N+ DF+P +   D+QG   + K  GK  D 
Sbjct: 188 LLGRTKD----DRYDLKGIMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDK 247

Query: 251 FLERIVEEHVV-FERDNKDKDFVDVMLDLMGSREYQ-------IDRSNIKAIILDFLIAA 310
            LE I++EH       N   DF+D ML L              IDRS IKAI++D + AA
Sbjct: 248 ILEFIIDEHEQNSSNGNASGDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISAA 307

Query: 311 VDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRM 370
           +D++ T+I W+L+ELIKHP  MKK QEE++ VVG++RMVEE+DL NL+Y+ MV+KE LR+
Sbjct: 308 IDTSDTSIEWILTELIKHPRAMKKCQEEIDAVVGVDRMVEETDLPNLEYVYMVVKEGLRL 367

Query: 371 HPPVPLI-PRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVD-PHKFDPERFLESEM 430
           HP  PL+ P E ++D  INGY IP +SR+++N+WA+GRDPN W +   +F PERF  S +
Sbjct: 368 HPVAPLLGPHESMEDITINGYFIPKQSRVIVNSWALGRDPNVWSENAEEFLPERFEGSNV 427

Query: 431 DVKGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILG 490
           DV+GRDF+L+PFGSGRRGC G+QL L+ V+ VVA+LVHCFD  LPNG +P  LDMTE  G
Sbjct: 428 DVRGRDFQLLPFGSGRRGCPGMQLGLITVQLVVARLVHCFDWNLPNGTTPDNLDMTEKFG 487

Query: 491 LTCSRAQNLTVIPIFRL 495
           LT  R ++L  +P +RL
Sbjct: 488 LTTPRVKHLLAVPKYRL 500

BLAST of Chy2G029520 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 377.5 bits (968), Expect = 2.4e-103
Identity = 203/483 (42.03%), Postives = 296/483 (61.28%), Query Frame = 0

Query: 9   ITLIFLLPCLFQQWLLKTKPLCNK---LPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYG 68
           ++L+FL   L   +LLK      K   LPP P   PIIG+LH LG L H+ L  L+   G
Sbjct: 5   VSLLFLAIAL-TFFLLKLNEKREKKPNLPPSPPNLPIIGNLHQLGNLPHRSLRSLANELG 64

Query: 69  PIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYGPYW 128
           P++ + LG +P +IVS+A   E  LKTHDL FA RP T  +  I Y    + F+ YG YW
Sbjct: 65  PLILLHLGHIPTLIVSTAEIAEEILKTHDLIFASRPSTTAARRIFYDCTDVAFSPYGEYW 124

Query: 129 RNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVDMIC 188
           R +RK+C LELLS  ++N + S+R++EVG ++E +  + + G AV+L+  +  +    I 
Sbjct: 125 RQVRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTIT 184

Query: 189 VMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDV-QGFGGRAKAVGKI 248
            +  G+KYE  E  +  F  L  E T L  A  +GD+ P  A  DV  G   R K     
Sbjct: 185 RVAFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGE 244

Query: 249 FDVFLERIVEEHVVFERDN-----KDKDFVDVMLDLM--GSREYQIDRSNIKAIILDFLI 308
            D F++ ++++H++  + N     + KD VDV+L L    S    ++R+N+KA+ILD   
Sbjct: 245 LDAFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFS 304

Query: 309 AAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESL 368
              D+T  T+ W ++ELIKHP +M+K Q+E+ +VVG    VEE DL  L YL+++IKE+L
Sbjct: 305 GGTDTTAVTLEWAMAELIKHPDVMEKAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETL 364

Query: 369 RMHPPVP-LIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESE 428
           R+HP  P L+PRE  +D  I GYHIP K+R+ INAWAIGRDP +W +  +F PERF+ + 
Sbjct: 365 RLHPVAPLLVPRESTRDVVIRGYHIPAKTRVFINAWAIGRDPKSWENAEEFLPERFVNNS 424

Query: 429 MDVKGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEIL 480
           +D KG+DF+LIPFG+GRRGC GI   +  V   +A L++ F+ +LP  ++  +LDM+E +
Sbjct: 425 VDFKGQDFQLIPFGAGRRGCPGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAV 484

BLAST of Chy2G029520 vs. ExPASy Swiss-Prot
Match: A0A068Q721 (Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 5.3e-103
Identity = 199/485 (41.03%), Postives = 298/485 (61.44%), Query Frame = 0

Query: 11  LIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIMHI 70
           LI LL  +++    K++    KLPP P   P+IG+LH LG   H  L  L++ YGPI+++
Sbjct: 29  LILLLKFIYKD---KSRKRRVKLPPSPPKLPVIGNLHQLGNKPHLSLRCLAEKYGPIIYL 88

Query: 71  QLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYGPYWRNIRK 130
           QLG +P ++VSSAR  +  LKTHDL  + RP   ++ H+ Y    + F+ YG YWR+IRK
Sbjct: 89  QLGEIPTVVVSSARLAKEVLKTHDLALSSRPQIFSAKHLFYNCTDVVFSPYGAYWRHIRK 148

Query: 131 MCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVDMICVMVLG 190
           +C LELLS+ ++  FS +R++EV  L+    VA       +LT  +     D++C +  G
Sbjct: 149 ICILELLSAKRVQSFSHVREEEVARLVR--RVAEFYPGTTNLTKMLGLYANDVLCRVAFG 208

Query: 191 RKY-EDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARF-DVQGFGGRAKAVGKIFDVF 250
           R + E  + D  GF+ ++ E  +L    ++GDF P +     + G   R +   + FD  
Sbjct: 209 RGFSEGGDYDRHGFQKMLEEYQELLGGFSIGDFFPSMEFIHSLTGMKSRLQETFRRFDEL 268

Query: 251 LERIVEEHVVFERDNKD-KDFVDVMLDLM--GSREYQIDRSNIKAIILDFLIAAVDSTTT 310
            +++V +H+  +R+ ++ KD VDV+LD+    S E  +   N+KAIILD   A  D+T  
Sbjct: 269 FDQMVTDHLSPKREKEEHKDLVDVLLDIQKKESTEMPLTMDNVKAIILDMFAAGTDTTFI 328

Query: 311 TINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMHPPVP- 370
           T++W ++EL+ +  ++++ Q E+  VVG  R+V ESDL  L Y++ VIKE  R+HPP P 
Sbjct: 329 TLDWGMTELLMNRKVLERAQAEVRGVVGERRVVLESDLPQLDYMKAVIKEIFRLHPPAPV 388

Query: 371 LIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVKGRDF 430
           L+PRE ++D  I+GY I  K+RI +NAWAIGRDP +W DP  F+PERF+ S +D KG+DF
Sbjct: 389 LVPRESMEDVTIDGYDILAKTRIFVNAWAIGRDPESWEDPEAFEPERFIGSTIDFKGQDF 448

Query: 431 ELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTCSRAQ 490
           ELIPFG+GRRGC  +      +   +AQL+H FD +LP   +  +LDMTE+ G+T  R  
Sbjct: 449 ELIPFGAGRRGCPAVTFGTATIELALAQLLHTFDWELPLDTAAKDLDMTEVFGITMHRIA 508

BLAST of Chy2G029520 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 2.2e-101
Identity = 191/477 (40.04%), Postives = 296/477 (62.05%), Query Frame = 0

Query: 30  CNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIMHIQLGLLPAIIVSSARATELF 89
           C  LPPGP G+PI+G+L  LG   H+ L  LS+ YGP+  ++LG +  ++ SS+     F
Sbjct: 30  CPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQYGPLFRLRLGSVNVVVASSSAVAAQF 89

Query: 90  LKTHDLHFARRPLTITSNHISYARKGITFAQYGPYWRNIRKMCTLELLSSLKINYFSSMR 149
           L+THD++F+ RP    + H++Y  + + FA YGP WR +RK+C++ L S   ++    +R
Sbjct: 90  LRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRMLRKLCSVHLFSLKALDDLRPVR 149

Query: 150 KQEVGSLIEGLEVAATDGVAVDLTSTISCVIVDMICVMVLGRKY---EDSELDEKG-FKG 209
           + EV  L+  L   A  GV V+L   ++    + +   +LGR+    ED++L E   FK 
Sbjct: 150 QGEVACLVRNLRRHADTGVLVNLGKALNVCATNALARAMLGRRVFADEDAQLAEADEFKE 209

Query: 210 LIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVGKIFDVFLERIVEEHVVFERDNKD 269
           ++ E  +LA   N+GDF+P +   D+QG  G+ K + + +D FL+R++EE+   + + K 
Sbjct: 210 MVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRLHRRYDAFLDRVIEEN---QANAKS 269

Query: 270 KDFVDVMLDLM---GSREYQIDRSNIKAIILDFLIAAVDSTTTTINWMLSELIKHPHIMK 329
            D + V++ L       E +++ ++IKA++L+   A  D++++T+ W+L+ELI+HP I++
Sbjct: 270 GDLLSVLIRLKEADAEGEIKLNNTDIKALLLNLFTAGTDTSSSTVEWVLAELIRHPDILQ 329

Query: 330 KLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMHPPVPL-IPRECIQDCNINGYHI 389
           K Q EL+ V+G +R+V ESDL NL YL+ V+KE+ R+HP  PL +PR   ++C +NGY I
Sbjct: 330 KTQHELDSVIGRDRLVAESDLPNLPYLQAVVKETFRLHPSTPLSLPRMASEECIVNGYKI 389

Query: 390 PVKSRIVINAWAIGRDPNTWVDPHKFDPERFL----ESEMDVKGRDFELIPFGSGRRGCV 449
           P  + +++N W+IGRD   W DP +F P RFL       +DVKG DFE+IPFG+GRR C 
Sbjct: 390 PKHATLLVNVWSIGRDAAVWNDPLEFRPSRFLPGGEREHVDVKGNDFEVIPFGAGRRICA 449

Query: 450 GIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTCSRAQNLTVIPIFRL 495
           G+ L L +V+ + A +VH +D  LP G    +LDM E  GLT  RA  L V PI RL
Sbjct: 450 GLSLGLRMVQFMTATIVHAYDWSLPKGQECQKLDMEEAYGLTLQRAVPLMVQPIPRL 503

BLAST of Chy2G029520 vs. ExPASy TrEMBL
Match: A0A5D3CM91 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002430 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.9e-269
Identity = 471/499 (94.39%), Postives = 481/499 (96.39%), Query Frame = 0

Query: 1   MTWIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIIL TITLIFLLP LFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFA RPLTITSNHISY RKGI FAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVI 180
           YGPYWRNIRKMCTLELLSSLKIN FSSMRKQEVGSLI+GLE AATDGVAVDLTS IS VI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 VDMICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
            DMICVMVLGRKYED+EL EKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSREYQIDRSNIKAIILDFLIAAV 300
           VGKIFD FLE IVEEHVVFE+DNKDKDFVDV+LDLMGSREYQIDRSNIKAIILDF+IAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMV+KESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVK 420
           PPVPLIPRECIQDCNINGYHIP KSRIVINAWAIGRDPNTWVDPHKFDPERFLESE+DV+
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVR VVAQL+HCFDLKLP+GMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 SRAQNLTVIPIFRLCRNYL 500
            RAQNL VIPIFRLC NYL
Sbjct: 481 PRAQNLRVIPIFRLCPNYL 499

BLAST of Chy2G029520 vs. ExPASy TrEMBL
Match: A0A1S3B2K5 (cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485291 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.9e-269
Identity = 471/499 (94.39%), Postives = 481/499 (96.39%), Query Frame = 0

Query: 1   MTWIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIIL TITLIFLLP LFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFA RPLTITSNHISY RKGI FAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVI 180
           YGPYWRNIRKMCTLELLSSLKIN FSSMRKQEVGSLI+GLE AATDGVAVDLTS IS VI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 VDMICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
            DMICVMVLGRKYED+EL EKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSREYQIDRSNIKAIILDFLIAAV 300
           VGKIFD FLE IVEEHVVFE+DNKDKDFVDV+LDLMGSREYQIDRSNIKAIILDF+IAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMV+KESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVK 420
           PPVPLIPRECIQDCNINGYHIP KSRIVINAWAIGRDPNTWVDPHKFDPERFLESE+DV+
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVR VVAQL+HCFDLKLP+GMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 SRAQNLTVIPIFRLCRNYL 500
            RAQNL VIPIFRLC NYL
Sbjct: 481 PRAQNLRVIPIFRLCPNYL 499

BLAST of Chy2G029520 vs. ExPASy TrEMBL
Match: A0A5A7T3X6 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold18G00600 PE=3 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 1.6e-267
Identity = 469/499 (93.99%), Postives = 479/499 (95.99%), Query Frame = 0

Query: 1   MTWIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIIL TITLIFLLP LFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFA RPLTITSNHISY RKGI FAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIAFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVI 180
           YGPYWRNIRKMCTLELLSSLKIN FSS RKQEVGSLI+GLE AATDGVAVDLTS IS VI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSTRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 VDMICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
            DMICVMVLGRKYED+EL EKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSREYQIDRSNIKAIILDFLIAAV 300
           VGKIFD FLE IVEEHVVFE+DNKDKDFVDV+LDLMGSREYQIDRSNIKAIILDF+IAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMV+KESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVK 420
           PPVPLIPRECIQDCNINGYHIP KSRIVINAWAIGRDPNTWVDPHKFDPERFLESE+DV+
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVR VVAQL+HCFDLKLP+GMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 SRAQNLTVIPIFRLCRNYL 500
            RAQNL VIPIFRLC N L
Sbjct: 481 PRAQNLRVIPIFRLCPNNL 499

BLAST of Chy2G029520 vs. ExPASy TrEMBL
Match: A0A6J1BYU0 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.5e-177
Identity = 310/497 (62.37%), Postives = 391/497 (78.67%), Query Frame = 0

Query: 1   MTWIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           M WI+L  + L  L   + + WL +++   NKLPPGP+GFP+ GSLHLLGKL H+DL  L
Sbjct: 1   MAWILL-AVALAGLALLILKPWLFRSQ---NKLPPGPRGFPVFGSLHLLGKLPHRDLRDL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQ 120
           SQ YG IM+++LG++P IIV+S +A ELFLKTHDL FA RP    S ++SY +K + FAQ
Sbjct: 61  SQKYGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVI 180
           YG YWRN+RKMCTLELLS  K+N F SMR++EVG L+  L  AA D V ++L+S I+ + 
Sbjct: 121 YGSYWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLN 180

Query: 181 VDMICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
            DM C+MV G+KY D+E DE+GFK +I+EA QL A PNLGDFIP IA  D+QG   R+KA
Sbjct: 181 TDMTCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKA 240

Query: 241 VGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSR--EYQIDRSNIKAIILDFLIA 300
           + KIFD F E+I++EH+  + +NK KDFVDVMLD+MGS+  EYQIDRS+IKAIILD L+A
Sbjct: 241 ISKIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVA 300

Query: 301 AVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLR 360
           A+D++ TT+ W ++ELIKHP +MKKLQEEL+KVVGL+R VEESDL +L+YL+M +KE LR
Sbjct: 301 AMDTSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILR 360

Query: 361 MHPPVP-LIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEM 420
           +HP  P L+P E ++DC +NGYHIP KSRI++NAWAIG+DPN+WVDP KF PERF+ S++
Sbjct: 361 VHPAAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQV 420

Query: 421 DVKGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILG 480
           DVKGRDF+LIPFGSGRRGC G+ + LVVV  V+AQLVHCFD KLPNGM P ELDMTE  G
Sbjct: 421 DVKGRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFG 480

Query: 481 LTCSRAQNLTVIPIFRL 495
           LTC RAQ+L V PI+RL
Sbjct: 481 LTCPRAQDLMVTPIYRL 493

BLAST of Chy2G029520 vs. ExPASy TrEMBL
Match: A0A6J1BTE3 (cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005393 PE=3 SV=1)

HSP 1 Score: 632.5 bits (1630), Expect = 1.5e-177
Identity = 314/495 (63.43%), Postives = 389/495 (78.59%), Query Frame = 0

Query: 3   WIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQ 62
           W+I+T + L      LF  WL   +    +LPPGPKGFPI GSLHLLGKL H+ LH LSQ
Sbjct: 5   WLIITILPLFL----LFSSWLWVLRSHA-RLPPGPKGFPIFGSLHLLGKLPHRALHRLSQ 64

Query: 63  IYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYG 122
            YGPIMH++LGL+  I+VSS  A ELFLKTHD  FA RPL   SNHISY RKG+ FA+YG
Sbjct: 65  KYGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYG 124

Query: 123 PYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVD 182
           PYWRNIRKMCT+ELLS+LK++ F SMR +EVG LI+ L  AA   V V+L+S ++ ++ D
Sbjct: 125 PYWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVAD 184

Query: 183 MICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVG 242
           M C+MV G+KY D +LDE+GFK +I+E  QLAAAPNLGDFIP +A  D QGF  RAK++ 
Sbjct: 185 MTCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSIS 244

Query: 243 KIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSR--EYQIDRSNIKAIILDFLIAAV 302
           K+ D FLERI++EH+  +  NK KDFVDVMLDLM S+  EYQI+RS+IKAIILD L AAV
Sbjct: 245 KVIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQATEYQIERSSIKAIILDLLAAAV 304

Query: 303 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMH 362
           D++ TTI+W LSELIKHP  MKK+Q+ELEKVVGL+RMVE+SDL NLKYL+MVIKE  RMH
Sbjct: 305 DTSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMH 364

Query: 363 PPVP-LIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDV 422
           PP P L+P E + DC ++ ++IP KSRI++NAWAIGRDPN W+D  KF PERF+ S++D+
Sbjct: 365 PPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDI 424

Query: 423 KGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLT 482
           KGRDF+LIPFGSGRRGC G+Q+ L+VVR V+AQLVHCF+ +LPNG  P +L+MTE  GLT
Sbjct: 425 KGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLT 484

Query: 483 CSRAQNLTVIPIFRL 495
           C RA++L VIPI+R+
Sbjct: 485 CPRAEDLKVIPIYRI 494

BLAST of Chy2G029520 vs. NCBI nr
Match: XP_008441066.1 (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >TYK12655.1 cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 939 bits (2427), Expect = 0.0
Identity = 471/499 (94.39%), Postives = 481/499 (96.39%), Query Frame = 0

Query: 1   MTWIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIIL TITLIFLLP LFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFA RPLTITSNHISY RKGI FAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIVFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVI 180
           YGPYWRNIRKMCTLELLSSLKIN FSSMRKQEVGSLI+GLE AATDGVAVDLTS IS VI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSMRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 VDMICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
            DMICVMVLGRKYED+EL EKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSREYQIDRSNIKAIILDFLIAAV 300
           VGKIFD FLE IVEEHVVFE+DNKDKDFVDV+LDLMGSREYQIDRSNIKAIILDF+IAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMV+KESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVK 420
           PPVPLIPRECIQDCNINGYHIP KSRIVINAWAIGRDPNTWVDPHKFDPERFLESE+DV+
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVR VVAQL+HCFDLKLP+GMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 SRAQNLTVIPIFRLCRNYL 499
            RAQNL VIPIFRLC NYL
Sbjct: 481 PRAQNLRVIPIFRLCPNYL 499

BLAST of Chy2G029520 vs. NCBI nr
Match: KAA0036261.1 (cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 933 bits (2412), Expect = 0.0
Identity = 469/499 (93.99%), Postives = 479/499 (95.99%), Query Frame = 0

Query: 1   MTWIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           MTWIIL TITLIFLLP LFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL
Sbjct: 1   MTWIILATITLIFLLPYLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQ 120
           SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFA RPLTITSNHISY RKGI FAQ
Sbjct: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFASRPLTITSNHISYGRKGIAFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVI 180
           YGPYWRNIRKMCTLELLSSLKIN FSS RKQEVGSLI+GLE AATDGVAVDLTS IS VI
Sbjct: 121 YGPYWRNIRKMCTLELLSSLKINSFSSTRKQEVGSLIKGLEDAATDGVAVDLTSKISSVI 180

Query: 181 VDMICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
            DMICVMVLGRKYED+EL EKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA
Sbjct: 181 GDMICVMVLGRKYEDNELGEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240

Query: 241 VGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSREYQIDRSNIKAIILDFLIAAV 300
           VGKIFD FLE IVEEHVVFE+DNKDKDFVDV+LDLMGSREYQIDRSNIKAIILDF+IAAV
Sbjct: 241 VGKIFDGFLESIVEEHVVFEKDNKDKDFVDVLLDLMGSREYQIDRSNIKAIILDFVIAAV 300

Query: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMH 360
           DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMV+KESLRMH
Sbjct: 301 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVMKESLRMH 360

Query: 361 PPVPLIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVK 420
           PPVPLIPRECIQDCNINGYHIP KSRIVINAWAIGRDPNTWVDPHKFDPERFLESE+DV+
Sbjct: 361 PPVPLIPRECIQDCNINGYHIPKKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEVDVR 420

Query: 421 GRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTC 480
           GRDFELIPFGSGRRGCVGIQLALVVVR VVAQL+HCFDLKLP+GMSPLELDMTEILGLTC
Sbjct: 421 GRDFELIPFGSGRRGCVGIQLALVVVRLVVAQLLHCFDLKLPDGMSPLELDMTEILGLTC 480

Query: 481 SRAQNLTVIPIFRLCRNYL 499
            RAQNL VIPIFRLC N L
Sbjct: 481 PRAQNLRVIPIFRLCPNNL 499

BLAST of Chy2G029520 vs. NCBI nr
Match: XP_023543453.1 (cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 704 bits (1818), Expect = 5.17e-251
Identity = 357/493 (72.41%), Postives = 409/493 (82.96%), Query Frame = 0

Query: 4   IILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQI 63
           +IL T+ LI LL  L Q W+ K+    NKLPPGPKGFPIIGSLHLL KLIH+DLH+LSQ 
Sbjct: 3   LILATMILISLLTYLIQGWVFKSNTFGNKLPPGPKGFPIIGSLHLLSKLIHRDLHHLSQR 62

Query: 64  YGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYGP 123
           YGPIM++QLG + AI+VSS RA ELFLKTHDL F  RPLT TSN ISY RKGI F+QY  
Sbjct: 63  YGPIMYMQLGRVHAIVVSSPRAAELFLKTHDLDFPSRPLTKTSNQISYGRKGIAFSQYDS 122

Query: 124 YWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVDM 183
           +WRN+RKMCTLEL S LKIN FSSMRKQEV  L++ L+ AA + VAVDL+S ISC+IVD+
Sbjct: 123 HWRNMRKMCTLELFSFLKINSFSSMRKQEVRLLVDDLQEAAENRVAVDLSSKISCLIVDI 182

Query: 184 ICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVGK 243
           +C+MV+GRKY D ELD KGFK +I+EAT+LAAAPNLGDF P IAR D+QG   RAK+VG 
Sbjct: 183 MCLMVVGRKYRDKELDAKGFKAVIQEATRLAAAPNLGDFFPFIARLDLQGLCRRAKSVGD 242

Query: 244 IFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSRE--YQIDRSNIKAIILDFLIAAVD 303
           +FD FLE+IVEEHVV + D K+KDFVDVMLDLMGSRE  YQID S IKA+ILD LIAAVD
Sbjct: 243 VFDGFLEKIVEEHVVSKND-KNKDFVDVMLDLMGSRENDYQIDCSTIKAMILDLLIAAVD 302

Query: 304 STTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMHP 363
           S+TT INW L ELIKHPHIMKK+QEELEKVVGLNRMVEESDL+NLKYLEMVIKE+ RMHP
Sbjct: 303 SSTTIINWALPELIKHPHIMKKMQEELEKVVGLNRMVEESDLNNLKYLEMVIKETFRMHP 362

Query: 364 PVPLIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVKG 423
           P+PLIPRE IQDC +NGYHIP KSR+VINAWAIGRD  TW+DP +F PERF++ ++D+KG
Sbjct: 363 PIPLIPRESIQDCMVNGYHIPNKSRLVINAWAIGRDARTWLDPDEFIPERFVDEQVDMKG 422

Query: 424 RDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTCS 483
           +DF+LIPFG GRRGC G QLAL  VR VVAQLVHCFD +LPNGM   ELDMTE  GLTC 
Sbjct: 423 KDFKLIPFGYGRRGCPGTQLALTTVRLVVAQLVHCFDWELPNGMMQAELDMTEEFGLTCP 482

Query: 484 RAQNLTVIPIFRL 494
           RAQNL +IP +RL
Sbjct: 483 RAQNLVLIPRYRL 494

BLAST of Chy2G029520 vs. NCBI nr
Match: XP_022133318.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 634 bits (1635), Expect = 2.84e-223
Identity = 310/497 (62.37%), Postives = 391/497 (78.67%), Query Frame = 0

Query: 1   MTWIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYL 60
           M WI+L  + L  L   + + WL +++   NKLPPGP+GFP+ GSLHLLGKL H+DL  L
Sbjct: 1   MAWILLA-VALAGLALLILKPWLFRSQ---NKLPPGPRGFPVFGSLHLLGKLPHRDLRDL 60

Query: 61  SQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQ 120
           SQ YG IM+++LG++P IIV+S +A ELFLKTHDL FA RP    S ++SY +K + FAQ
Sbjct: 61  SQKYGSIMYMRLGIVPTIIVTSPQAAELFLKTHDLVFASRPFFEASKYMSYGQKNLGFAQ 120

Query: 121 YGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVI 180
           YG YWRN+RKMCTLELLS  K+N F SMR++EVG L+  L  AA D V ++L+S I+ + 
Sbjct: 121 YGSYWRNMRKMCTLELLSHHKVNSFRSMRREEVGLLVHYLRDAAKDHVVINLSSKIASLN 180

Query: 181 VDMICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKA 240
            DM C+MV G+KY D+E DE+GFK +I+EA QL A PNLGDFIP IA  D+QG   R+KA
Sbjct: 181 TDMTCLMVFGKKYADAEFDERGFKSVIQEAMQLVATPNLGDFIPCIAALDLQGLNRRSKA 240

Query: 241 VGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSRE--YQIDRSNIKAIILDFLIA 300
           + KIFD F E+I++EH+  + +NK KDFVDVMLD+MGS+E  YQIDRS+IKAIILD L+A
Sbjct: 241 ISKIFDGFFEKIIDEHLESKNENKTKDFVDVMLDIMGSQETEYQIDRSSIKAIILDMLVA 300

Query: 301 AVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLR 360
           A+D++ TT+ W ++ELIKHP +MKKLQEEL+KVVGL+R VEESDL +L+YL+M +KE LR
Sbjct: 301 AMDTSATTVGWAIAELIKHPQVMKKLQEELQKVVGLDRRVEESDLDHLEYLDMFVKEILR 360

Query: 361 MHPPVPL-IPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEM 420
           +HP  PL +P E ++DC +NGYHIP KSRI++NAWAIG+DPN+WVDP KF PERF+ S++
Sbjct: 361 VHPAAPLLVPHESLEDCTVNGYHIPKKSRIIVNAWAIGQDPNSWVDPEKFFPERFIGSQV 420

Query: 421 DVKGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILG 480
           DVKGRDF+LIPFGSGRRGC G+ + LVVV  V+AQLVHCFD KLPNGM P ELDMTE  G
Sbjct: 421 DVKGRDFQLIPFGSGRRGCPGMHMGLVVVHFVIAQLVHCFDWKLPNGMLPAELDMTEEFG 480

Query: 481 LTCSRAQNLTVIPIFRL 494
           LTC RAQ+L V PI+RL
Sbjct: 481 LTCPRAQDLMVTPIYRL 493

BLAST of Chy2G029520 vs. NCBI nr
Match: XP_022132564.1 (cytochrome P450 CYP736A12-like [Momordica charantia])

HSP 1 Score: 634 bits (1634), Expect = 4.03e-223
Identity = 314/495 (63.43%), Postives = 389/495 (78.59%), Query Frame = 0

Query: 3   WIILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQ 62
           W+I+T + L  L    F  WL   +    +LPPGPKGFPI GSLHLLGKL H+ LH LSQ
Sbjct: 5   WLIITILPLFLL----FSSWLWVLRSHA-RLPPGPKGFPIFGSLHLLGKLPHRALHRLSQ 64

Query: 63  IYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYG 122
            YGPIMH++LGL+  I+VSS  A ELFLKTHD  FA RPL   SNHISY RKG+ FA+YG
Sbjct: 65  KYGPIMHLRLGLVSTIVVSSPAAAELFLKTHDHVFASRPLIQASNHISYGRKGLGFAEYG 124

Query: 123 PYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVD 182
           PYWRNIRKMCT+ELLS+LK++ F SMR +EVG LI+ L  AA   V V+L+S ++ ++ D
Sbjct: 125 PYWRNIRKMCTVELLSNLKVSSFRSMRSEEVGLLIDHLREAAKSDVVVNLSSKVTSLVAD 184

Query: 183 MICVMVLGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFDVQGFGGRAKAVG 242
           M C+MV G+KY D +LDE+GFK +I+E  QLAAAPNLGDFIP +A  D QGF  RAK++ 
Sbjct: 185 MTCLMVFGKKYGDEQLDERGFKAVIQEVMQLAAAPNLGDFIPYVAGLDFQGFNRRAKSIS 244

Query: 243 KIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSR--EYQIDRSNIKAIILDFLIAAV 302
           K+ D FLERI++EH+  +  NK KDFVDVMLDLM S+  EYQI+RS+IKAIILD L AAV
Sbjct: 245 KVIDGFLERIIDEHLQPKIGNKTKDFVDVMLDLMNSQATEYQIERSSIKAIILDLLAAAV 304

Query: 303 DSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMH 362
           D++ TTI+W LSELIKHP  MKK+Q+ELEKVVGL+RMVE+SDL NLKYL+MVIKE  RMH
Sbjct: 305 DTSATTISWALSELIKHPLSMKKIQDELEKVVGLDRMVEDSDLCNLKYLDMVIKEIFRMH 364

Query: 363 PPVPL-IPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDV 422
           PP PL +P E + DC ++ ++IP KSRI++NAWAIGRDPN W+D  KF PERF+ S++D+
Sbjct: 365 PPAPLLVPHESLDDCTVDNFYIPKKSRIIVNAWAIGRDPNVWIDAEKFFPERFIGSQVDI 424

Query: 423 KGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLT 482
           KGRDF+LIPFGSGRRGC G+Q+ L+VVR V+AQLVHCF+ +LPNG  P +L+MTE  GLT
Sbjct: 425 KGRDFQLIPFGSGRRGCPGMQMGLIVVRLVLAQLVHCFNWELPNGTLPTKLNMTEEFGLT 484

Query: 483 CSRAQNLTVIPIFRL 494
           C RA++L VIPI+R+
Sbjct: 485 CPRAEDLKVIPIYRI 494

BLAST of Chy2G029520 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 363.6 bits (932), Expect = 2.5e-100
Identity = 202/510 (39.61%), Postives = 304/510 (59.61%), Query Frame = 0

Query: 1   MTWIILTTI--TLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLH 60
           M  + LT +  T++FL+  +F     + +   N+LPPGP  +PIIG+L  +G   H+ L 
Sbjct: 1   MATLFLTILLATVLFLILRIFSH--RRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLS 60

Query: 61  YLSQIYGPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITF 120
            +   YGPI+H++LG +  ++ +S    E FLK HD +FA RP    + H++Y  + + F
Sbjct: 61  AMVTTYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVF 120

Query: 121 AQYGPYWRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISC 180
           A YG  WR +RK+ ++ L S+  +  F  +R++EVG+L   L    T    V+L   ++ 
Sbjct: 121 APYGHRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTK--PVNLGQLVNM 180

Query: 181 VIVDMICVMVLGRKYEDSELDEKG--FKGLIREATQLAAAPNLGDFIPLIARFDVQGFGG 240
            +V+ +   ++GR+   ++ D K   F+ ++ E   LA   N+GDF+P +   D+QG  G
Sbjct: 181 CVVNALGREMIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAG 240

Query: 241 RAKAVGKIFDVFLERIVEEHVVFERDNKDKDFVDVMLDLMGS----REYQIDRSNIKAII 300
           + K + K FD FL  I++EH +  +D K  D +  ++ L G+        +  + IKA++
Sbjct: 241 KMKRLHKRFDAFLSSILKEHEMNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALL 300

Query: 301 LDFLIAAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMV 360
           L+   A  D++ +T++W ++ELI+HP IM K QEEL+ VVG +R V ESD++ L YL+ V
Sbjct: 301 LNMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAV 360

Query: 361 IKESLRMHPPVPL-IPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPER 420
           IKE+ R+HPP PL +P    + C INGYHIP  S ++ N WAI RDP+ W DP  F PER
Sbjct: 361 IKENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPER 420

Query: 421 FL----ESEMDVKGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSP 480
           FL    +S +DVKG DFELIPFG+GRR C G+ L L  ++ + A LV  FD +L  G++P
Sbjct: 421 FLPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTP 480

Query: 481 LELDMTEILGLTCSRAQNLTVIPIFRLCRN 498
            +L+M E  GLT  RA  L V P  RL  N
Sbjct: 481 EKLNMEESYGLTLQRAVPLVVHPKPRLAPN 506

BLAST of Chy2G029520 vs. TAIR 10
Match: AT3G26300.1 (cytochrome P450, family 71, subfamily B, polypeptide 34 )

HSP 1 Score: 351.7 bits (901), Expect = 9.9e-97
Identity = 182/489 (37.22%), Postives = 290/489 (59.30%), Query Frame = 0

Query: 9   ITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIM 68
           ++LIF++  L   +  K +    + PP P G PIIG+LH LG+L H+ L  LS+ YGP+M
Sbjct: 7   LSLIFVICILVAVFNHKNRRNYQRTPPSPPGCPIIGNLHQLGELPHQSLWKLSKKYGPVM 66

Query: 69  HIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYGPYWRNI 128
            ++LG +P +IVSS+   +  LK HDLH   RP    +  +SY    I F+ Y  YW+ +
Sbjct: 67  LLKLGRVPTVIVSSSETAKQALKIHDLHCCSRPGFAGARELSYNYLDIAFSPYDDYWKEV 126

Query: 129 RKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVDMICVMV 188
           RK+   EL SS +++    ++ +EV  LI+ +  +A     ++L  T+  + V ++C   
Sbjct: 127 RKLAVQELFSSKQVHSIQPIKDEEVKKLIDSISESAAQKTPINLNKTLLALTVSVVCRTA 186

Query: 189 LGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIARFD--VQGFGGRAKAVGKIFD 248
               +E + L+ + F  ++REA ++  + +  DFIP + R    + G  GR +   +  D
Sbjct: 187 FSVNFEGTVLNSERFNNIVREALEMLGSFSASDFIPYVGRIIDLLTGLQGRRERSMRDLD 246

Query: 249 VFLERIVEEHVVFERDNKDKDFVDVMLDLMGSREY----QIDRSNIKAIILDFLIAAVDS 308
            F E++ + H   +++   +DFVD++L L          ++ R++IKAI++D L+A +D+
Sbjct: 247 AFYEQMFDLHKQ-KKEEGSEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAGMDT 306

Query: 309 TTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMHPP 368
           +  T+ W ++EL K+P +MKK+Q E+   +     +   D   L+YL+MVIKE+ R+HP 
Sbjct: 307 SAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRLHPT 366

Query: 369 VP-LIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVKG 428
            P LIPRE + +  INGY IPVK+R+ +N WAIGRDP+TW DP  F PERF ++ +D KG
Sbjct: 367 TPLLIPREAMSEFEINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFTDNNIDAKG 426

Query: 429 RDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTCS 488
           + FEL+PFG GRR C  + +   +V   +A L++ FD KLP GM   ++DM E  GLT +
Sbjct: 427 QHFELLPFGGGRRMCPAVYMGTTMVEFGLANLLYHFDWKLPEGMKVDDIDMEEAPGLTVN 486

Query: 489 RAQNLTVIP 491
           +   L ++P
Sbjct: 487 KKNELILVP 494

BLAST of Chy2G029520 vs. TAIR 10
Match: AT3G26310.1 (cytochrome P450, family 71, subfamily B, polypeptide 35 )

HSP 1 Score: 347.1 bits (889), Expect = 2.4e-95
Identity = 183/492 (37.20%), Postives = 295/492 (59.96%), Query Frame = 0

Query: 9   ITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIM 68
           + LIFL+  L   +  K  P   +  P P GFPIIG+LH +G+L H+ L  LS+ YGP+M
Sbjct: 7   LPLIFLVCILLAVFNHKKHPKYRQF-PCPPGFPIIGNLHQIGELPHQTLWKLSKKYGPVM 66

Query: 69  HIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYGPYWRNI 128
           H+ LG +P ++VSS+      L+ HDLH   RP       +SY    I F+ Y  YW+ +
Sbjct: 67  HLMLGRVPTVVVSSSDTARQVLRVHDLHCCTRPSLSGPRELSYNYLDIAFSPYDDYWKEV 126

Query: 129 RKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVDMICVMV 188
           RK+C  EL S+ +++    ++ +EV  +I+ +  +A+    V+L +    + V ++C   
Sbjct: 127 RKLCVQELFSTKQVHSIQPIKDEEVKKMIDSIAESASQKNPVNLNNKCLELTVSVVCRTA 186

Query: 189 LGRKYEDSELDEKGFKGLIREATQLAAAPNLGDFIPLIA-RFDV-QGFGGRAKAVGKIFD 248
            G  +E + L+   F  ++REA ++  + +  DFIP +    DV  G  GR +   +  +
Sbjct: 187 FGVSFEGTVLNSDRFNKIVREALEMLGSFSAADFIPYVGWIIDVLTGLQGRRERSKRDLN 246

Query: 249 VFLERIVEEHVVFERDNKDKDFVDVMLDLMGSREY----QIDRSNIKAIILDFLIAAVDS 308
            F E++ + H   +++  ++DFVD++L L          ++ R++IKAI+LD L+A +D+
Sbjct: 247 AFFEQMFDLHKEGKKEG-NEDFVDLLLRLEKEEAVLGNDKLTRNHIKAILLDVLLAGIDT 306

Query: 309 TTTTINWMLSELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMHPP 368
           +  T+ W ++EL ++P +MKK+Q E+   +G   M+   D+  L+YL+MVIKE+ R+HP 
Sbjct: 307 SAITMTWAMTELARNPRVMKKVQSEIRTQMGNRSMISFEDMDQLEYLKMVIKETWRLHPT 366

Query: 369 VP-LIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEMDVKG 428
            P L+PRE + + +INGY IPVK+R+ +N WAIGRDP+TW DP  F PERF+++ +D KG
Sbjct: 367 TPLLLPREAMSEFDINGYTIPVKTRLHVNVWAIGRDPDTWKDPEVFLPERFMDNNIDAKG 426

Query: 429 RDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTCS 488
           + FEL+PFG GRR C  I +   +V   +A L++ FD KLP G+   ++D+ E  GLT +
Sbjct: 427 QHFELLPFGGGRRICPAIYMGTTMVEFGLANLLYHFDWKLPEGVEVKDIDVEEAPGLTVN 486

Query: 489 RAQNLTVIPIFR 494
           +   L ++P  R
Sbjct: 487 KKNELLLVPEMR 496

BLAST of Chy2G029520 vs. TAIR 10
Match: AT4G36220.1 (ferulic acid 5-hydroxylase 1 )

HSP 1 Score: 344.7 bits (883), Expect = 1.2e-94
Identity = 189/484 (39.05%), Postives = 284/484 (58.68%), Query Frame = 0

Query: 34  PPGPKGFPIIGSLHLLGKLIHKDLHYLSQIYGPIMHIQLGLLPAIIVSSARATELFLKTH 93
           PPGP+G+PIIG++ ++ +L H+ L  L++ YG + H+++G L    VSS       L+  
Sbjct: 41  PPGPRGWPIIGNMLMMDQLTHRGLANLAKKYGGLCHLRMGFLHMYAVSSPEVARQVLQVQ 100

Query: 94  DLHFARRPLTITSNHISYARKGITFAQYGPYWRNIRKMCTLELLSSLKINYFSSMRKQEV 153
           D  F+ RP TI  ++++Y R  + FA YGP+WR +RK+C +++ S  +   ++S+R  EV
Sbjct: 101 DSVFSNRPATIAISYLTYDRADMAFAHYGPFWRQMRKVCVMKVFSRKRAESWASVR-DEV 160

Query: 154 GSLIEGLEVAATDGVAVDLTSTISCVIVDMICVMVLGRKYEDSELDEKGFKGLIREATQL 213
             ++    V+   G  +++   I  +  ++      G   E  + DE  F  +++E ++L
Sbjct: 161 DKMVR--SVSCNVGKPINVGEQIFALTRNITYRAAFGSACEKGQ-DE--FIRILQEFSKL 220

Query: 214 AAAPNLGDFIPLIARFDVQGFGGRAKAVGKIFDVFLERIVEEHV--------VFERDNKD 273
             A N+ DFIP     D QG   R        D F++ I++EH+        V + D  D
Sbjct: 221 FGAFNVADFIPYFGWIDPQGINKRLVKARNDLDGFIDDIIDEHMKKKENQNAVDDGDVVD 280

Query: 274 KDFVDVML--------------DLMGSREYQIDRSNIKAIILDFLIAAVDSTTTTINWML 333
            D VD +L              DL  S   ++ R NIKAII+D +    ++  + I W L
Sbjct: 281 TDMVDDLLAFYSEEAKLVSETADLQNS--IKLTRDNIKAIIMDVMFGGTETVASAIEWAL 340

Query: 334 SELIKHPHIMKKLQEELEKVVGLNRMVEESDLSNLKYLEMVIKESLRMHPPVPLIPRECI 393
           +EL++ P  +K++Q+EL +VVGL+R VEESD+  L YL+  +KE+LRMHPP+PL+  E  
Sbjct: 341 TELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLTYLKCTLKETLRMHPPIPLLLHETA 400

Query: 394 QDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERFLESEM-DVKGRDFELIPFG 453
           +D +I+G+ IP KSR++INA+AIGRDP +W DP  F P RFLE  + D KG +FE IPFG
Sbjct: 401 EDTSIDGFFIPKKSRVMINAFAIGRDPTSWTDPDTFRPSRFLEPGVPDFKGSNFEFIPFG 460

Query: 454 SGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDMTEILGLTCSRAQNLTVIP 495
           SGRR C G+QL L  +   VA ++HCF  KLP+GM P ELDM ++ GLT  +A  L  +P
Sbjct: 461 SGRRSCPGMQLGLYALDLAVAHILHCFTWKLPDGMKPSELDMNDVFGLTAPKATRLFAVP 516

BLAST of Chy2G029520 vs. TAIR 10
Match: AT1G13080.1 (cytochrome P450, family 71, subfamily B, polypeptide 2 )

HSP 1 Score: 342.4 bits (877), Expect = 6.0e-94
Identity = 181/501 (36.13%), Postives = 297/501 (59.28%), Query Frame = 0

Query: 5   ILTTITLIFLLPCLFQQWLLKTKPLCNKLPPGPKGFPIIGSLHLLGKLIHKDLHYLSQIY 64
           IL    L+ LL  +   +L + K     LPP P   PIIG+LH L  L H+  H LS  Y
Sbjct: 3   ILLCFFLVSLLTIVSSIFLKQNKTSKFNLPPSPSSLPIIGNLHHLAGLPHRCFHKLSIKY 62

Query: 65  GPIMHIQLGLLPAIIVSSARATELFLKTHDLHFARRPLTITSNHISYARKGITFAQYGPY 124
           GP++ ++LG +P +++SS+ A E  LKT+DL    RP T+ S  +SY  K ITFA YG Y
Sbjct: 63  GPLVFLRLGSVPVVVISSSEAAEAVLKTNDLECCSRPKTVGSGKLSYGFKDITFAPYGEY 122

Query: 125 WRNIRKMCTLELLSSLKINYFSSMRKQEVGSLIEGLEVAATDGVAVDLTSTISCVIVDMI 184
           WR +RK+  +EL SS K+  F  +R++EV  +++ +  +A     VDL+ T   +   +I
Sbjct: 123 WREVRKLAVIELFSSKKVQSFRYIREEEVDFVVKKVSESALKQSPVDLSKTFFSLTASII 182

Query: 185 CVMVLGRKYEDS--ELDEKGFKGLIREATQLAAAPNLGDFIP-LIARFDVQGFGGRAKAV 244
           C + LG+ + +S   +D+   + L+ E+ +        DF P  + RF V     R K +
Sbjct: 183 CRVALGQNFNESGFVIDQDRIEELVTESAEALGTFTFSDFFPGGLGRF-VDWLFQRHKKI 242

Query: 245 GKIF---DVFLERIVEEHVVFERDNKDKDFVDVMLDLMGSRE----YQIDRSNIKAIILD 304
            K+F   D F + ++++H+  E   K++D V ++LD++  +E    ++++  N+KAI++D
Sbjct: 243 NKVFKELDAFYQHVIDDHLKPE-GRKNQDIVTLILDMIDKQEDSDSFKLNMDNLKAIVMD 302

Query: 305 FLIAAVDSTTTTINWMLSELIKHPHIMKKLQEELEKVVGLNR-MVEESDLSNLKYLEMVI 364
             +A +D++  T+ W ++ELI++P +MKK QE +   +GL +  + E DL  ++YL  ++
Sbjct: 303 VFLAGIDTSAVTMIWAMTELIRNPRVMKKAQESIRTTLGLKKERITEEDLGKVEYLNHIL 362

Query: 365 KESLRMHPPVP-LIPRECIQDCNINGYHIPVKSRIVINAWAIGRDPNTWVDPHKFDPERF 424
           KE+ R+HP +P ++PRE +    I GY IP K++I +N W IGRDP  W DP +F+PERF
Sbjct: 363 KETFRLHPALPFVVPRETMSHIKIQGYDIPPKTQIQLNVWTIGRDPKRWNDPEEFNPERF 422

Query: 425 LESEMDVKGRDFELIPFGSGRRGCVGIQLALVVVRHVVAQLVHCFDLKLPNGMSPLELDM 484
             S +D +G+ F+L+PFGSGRR C G+ +A+  V   +  L++ FD  +P+G    ++DM
Sbjct: 423 ANSSVDFRGQHFDLLPFGSGRRICPGMPMAIASVELALMNLLYYFDWSMPDGTKGEDIDM 482

Query: 485 TEILGLTCSRAQNLTVIPIFR 494
            E   ++  +   L ++P+ R
Sbjct: 483 EEAGNISIVKKIPLQLVPVQR 501

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A0A068Q5V68.9e-15958.03Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1[more]
H2DH181.8e-12748.49Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1[more]
P244652.4e-10342.03Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2[more]
A0A068Q7215.3e-10341.03Cytochrome P450 71AP13 OS=Prunus mume OX=102107 GN=CYP71AP13 PE=2 SV=1[more]
A0A4D6Q4152.2e-10140.04Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
Match NameE-valueIdentityDescription
A0A5D3CM912.9e-26994.39Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3B2K52.9e-26994.39cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103485291 PE=3 SV=1[more]
A0A5A7T3X61.6e-26793.99Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1BYU01.5e-17762.37cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005927 PE... [more]
A0A6J1BTE31.5e-17763.43cytochrome P450 CYP736A12-like OS=Momordica charantia OX=3673 GN=LOC111005393 PE... [more]
Match NameE-valueIdentityDescription
XP_008441066.10.094.39PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo] >TYK12655.1 cytochrome ... [more]
KAA0036261.10.093.99cytochrome P450 CYP736A12-like protein [Cucumis melo var. makuwa][more]
XP_023543453.15.17e-25172.41cytochrome P450 CYP736A12-like [Cucurbita pepo subsp. pepo][more]
XP_022133318.12.84e-22362.37cytochrome P450 CYP736A12-like [Momordica charantia][more]
XP_022132564.14.03e-22363.43cytochrome P450 CYP736A12-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G07990.12.5e-10039.61Cytochrome P450 superfamily protein [more]
AT3G26300.19.9e-9737.22cytochrome P450, family 71, subfamily B, polypeptide 34 [more]
AT3G26310.12.4e-9537.20cytochrome P450, family 71, subfamily B, polypeptide 35 [more]
AT4G36220.11.2e-9439.05ferulic acid 5-hydroxylase 1 [more]
AT1G13080.16.0e-9436.13cytochrome P450, family 71, subfamily B, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 321..341
NoneNo IPR availablePANTHERPTHR47943CYTOCHROME P450 93A3-LIKEcoord: 1..494
NoneNo IPR availablePANTHERPTHR47943:SF2CYTOCHROME P450 FAMILY 71 PROTEINcoord: 1..494
IPR001128Cytochrome P450PRINTSPR00385P450coord: 298..315
score: 29.63
coord: 427..436
score: 57.4
coord: 436..447
score: 34.65
coord: 351..362
score: 42.66
IPR001128Cytochrome P450PFAMPF00067p450coord: 34..477
e-value: 2.2E-101
score: 339.9
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 436..459
score: 27.82
coord: 350..368
score: 31.45
coord: 287..304
score: 24.95
coord: 390..414
score: 40.81
coord: 179..197
score: 17.57
coord: 307..333
score: 37.39
coord: 61..80
score: 30.12
coord: 426..436
score: 53.79
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 27..493
e-value: 5.9E-125
score: 419.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 34..493
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 429..438

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G029520.1Chy2G029520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen