Chy2G029310 (gene) Cucumber (hystrix) v1

Overview
NameChy2G029310
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionvicilin-like seed storage protein At2g18540
LocationchrH02: 4031188 .. 4033537 (-)
RNA-Seq ExpressionChy2G029310
SyntenyChy2G029310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAAACGCACAGCAGTTTCAGTTTCAGGATCCTCGTTTTCACCTTCCATTCTTATCCCTATCTTCTTCCTCTTCCTCTCTCTTCCTGCATATGCCGATGACGGGTGGTGGGAAGGAGACAGTCCTGTGGTGAAGAGAGCCAATGAAAGAATCCCAATTCTTAAAACAGAGTACGGTGAGATCTCCGCGGTTGATTTCGACGATGGCACTCGATTTGGACGTTACCATCTTCAGTTCATCACATTGGAACCCAATTCACTGTTTCTCCCTGTTCTTCTTCATTCTGATATGGTGTTCTATGTCCACACTGGTATGGTATATGCTCTGTTTGTCGAAAAATTAGAAAAACAGAACGAAATATGAAATTTTGTATGACGAGATTTTTTATTTATTTTTTGTCGTTTTAGGAAGTGGGAGATTGAATTGGTTTGATGACAATGGTTTGAAGGAGGTGGATTTACGGCGGGGAGATCTTTATAGGCTTCATCCAGGCTCCATTTTTTACTTGCAGAGTAGCTTTGAGACCGAACGTGAAAAGCTTCGAATTTATGCTCTGTTTTCCAGCACAGATGAGGATTCATTCGTACGTTTCATCAAAAGTTATATCATTAAATCATTGAATGTTTGTAAATTGGTTTGAATATCTGATGGTGAGAATGAATTTGATGCATATCTTTGAAGAATCCTTCCATTGGAGCATACTCCCGCGTCACTGATCTGGTTCGTGGCTTCGGCAAAGAAGTTCTTCGTAAAGCCTTCATGGTATGAATTTGGCAAATCCAATTCACTCCCTAATTAGTTTATAACTGTCAAATTGTCAAATTTTCCATATTAGAACTTGTCAATTCAATTATTTTGTTTCGTTTTTGGATCATTTTATGAACGGTTGTGATAATGTGTAAAAAGGCCCCTGATGAAGTAATAGAGGAAATAATGAACACTAAGAGGCCGCCGCTTATCGTCCACGCTGCTGCGCCAACTCCGAGCATAAAGGCAAAGTCGTCGTCACCATGGGAATTTGAGGCTCGGTTATTGAAAACTTTCCTAGGAGGAGACGCAAGTGCGACAGAATTCAACAAGAAGAAGAAGAAGAAAGGCATATACAACGTTTATGAAGTAGACCCTGATTTTGAGAATTGCAATGGATGGAGTTTGACTGTAACTAAGAAAAACTCCCATCAATTGAAAGGCTCCAACATCGGCTTCCTCGTAGTCAACCTTACAGCGGTCAGTAATTAAATGTCACTGATTAATGAAAAAACAGAGTGAACAAACAAACAACAGAATTCATTTGCATTTGCAGGGATCAATGATGGGTCCGCATTGGAATCCGAGGGCGTGGGAGATTGGGATTGTGACATCGGACGAGCCGGGGGTGGTTCGTGTAGGGTGTTCGAGCACCTCGGCAAACAGTTCAAAATGCAAGAATTGGAGTTTTGTGGTAGAGAAAGGGGATGTATTTGTAGTGCCAAGGTTCCATCCAATGGCGCAAATGTCATTCAACAATGGAACGTTTGTATTTGTGGGATTTAGCACAACGAATGGACATAACATGCCGCAGTTCTTTGCTGGGAGTAGCTCTGTTTTGAAAATTGTGGACAGGGAAGTATTGGCATGGTCGTTTGATGTGAATGTGACAACGATTGATCGGTTGTTGAAAGCTAGAGTTGAGTCGATTGTTTTGGAATGTACTTCATGTGCTGAAGAGGAAGTAAGGAAAATGGAAGAGGAAGCTGAGAGAGAGAGGGAGGAGGAGGAAGAAAGAAAGAGAGAAGAGGAGGAAGAAGAGAGAAAAAGGGAAGAAGAGGAACGGAGGAAGAGAGAGGAAGAGGAAGAGAGGAAACGGGAAGAAGAAGAAGAGAGAAAAAGGGAAGAAGAGGAAGAGGAAGAGAGGAAACGGGAAGAAGAAGAAGAGAGAAAAAGGGAAGAAGAGGAAGAGGAAGAGAGGAAACGGGAAGAAGAAGAAGAGAGAAAAAGGGAAGAAGAGGAACGGAGGAAGAGAGAGGAAGAGGAAGAGAGGGAGAGAGAAGAAGAAGAAGCACAGAAAGAGGAAGAGTGGAGGAGAGAAGAGGAAGAGAGGAGAAAGAGAGAAGAGGAAGAGAGAAAAAGAGAGGAAGAGGAGACAGAGAGAGAGAAAGAGAGAGAAGAGGAGGAAGAGAGGGAGGCAGAGAGAGAGGAGGAGGAAGCTAGGGAGAGAGAGGAAGAACATCAAAGAGAGAGAGGGAAGAGGCGGAGAGAGGGGGAGGAAAGACAAAGAAGAGGGTGGGAGGAAGAAGAAGAAGAAGAAGAAGGTGGAGGAGAGGAGCCGCAGCTGCCAGTACTAAGAATTTTGGAACAATGGACTTGA

mRNA sequence

ATGAAGAAACGCACAGCAGTTTCAGTTTCAGGATCCTCGTTTTCACCTTCCATTCTTATCCCTATCTTCTTCCTCTTCCTCTCTCTTCCTGCATATGCCGATGACGGGTGGTGGGAAGGAGACAGTCCTGTGGTGAAGAGAGCCAATGAAAGAATCCCAATTCTTAAAACAGAGTACGGTGAGATCTCCGCGGTTGATTTCGACGATGGCACTCGATTTGGACGTTACCATCTTCAGTTCATCACATTGGAACCCAATTCACTGTTTCTCCCTGTTCTTCTTCATTCTGATATGGTGTTCTATGTCCACACTGGAAGTGGGAGATTGAATTGGTTTGATGACAATGGTTTGAAGGAGGTGGATTTACGGCGGGGAGATCTTTATAGGCTTCATCCAGGCTCCATTTTTTACTTGCAGAGTAGCTTTGAGACCGAACGTGAAAAGCTTCGAATTTATGCTCTGTTTTCCAGCACAGATGAGGATTCATTCAATCCTTCCATTGGAGCATACTCCCGCGTCACTGATCTGGTTCGTGGCTTCGGCAAAGAAGTTCTTCGTAAAGCCTTCATGGCCCCTGATGAAGTAATAGAGGAAATAATGAACACTAAGAGGCCGCCGCTTATCGTCCACGCTGCTGCGCCAACTCCGAGCATAAAGGCAAAGTCGTCGTCACCATGGGAATTTGAGGCTCGGTTATTGAAAACTTTCCTAGGAGGAGACGCAAGTGCGACAGAATTCAACAAGAAGAAGAAGAAGAAAGGCATATACAACGTTTATGAAGTAGACCCTGATTTTGAGAATTGCAATGGATGGAGTTTGACTGTAACTAAGAAAAACTCCCATCAATTGAAAGGCTCCAACATCGGCTTCCTCGTAGTCAACCTTACAGCGGGATCAATGATGGGTCCGCATTGGAATCCGAGGGCGTGGGAGATTGGGATTGTGACATCGGACGAGCCGGGGGTGGTTCGTGTAGGGTGTTCGAGCACCTCGGCAAACAGTTCAAAATGCAAGAATTGGAGTTTTGTGGTAGAGAAAGGGGATGTATTTGTAGTGCCAAGGTTCCATCCAATGGCGCAAATGTCATTCAACAATGGAACGTTTGTATTTGTGGGATTTAGCACAACGAATGGACATAACATGCCGCAGTTCTTTGCTGGGAGTAGCTCTGTTTTGAAAATTGTGGACAGGGAAGTATTGGCATGGTCGTTTGATGTGAATGTGACAACGATTGATCGGTTGTTGAAAGCTAGAGTTGAGTCGATTGTTTTGGAATGTACTTCATGTGCTGAAGAGGAAGTAAGGAAAATGGAAGAGGAAGCTGAGAGAGAGAGGGAGGAGGAGGAAGAAAGAAAGAGAGAAGAGGAGGAAGAAGAGAGAAAAAGGGAAGAAGAGGAACGGAGGAAGAGAGAGGAAGAGGAAGAGAGGAAACGGGAAGAAGAAGAAGAGAGAAAAAGGGAAGAAGAGGAAGAGGAAGAGAGGAAACGGGAAGAAGAAGAAGAGAGAAAAAGGGAAGAAGAGGAAGAGGAAGAGAGGAAACGGGAAGAAGAAGAAGAGAGAAAAAGGGAAGAAGAGGAACGGAGGAAGAGAGAGGAAGAGGAAGAGAGGGAGAGAGAAGAAGAAGAAGCACAGAAAGAGGAAGAGTGGAGGAGAGAAGAGGAAGAGAGGAGAAAGAGAGAAGAGGAAGAGAGAAAAAGAGAGGAAGAGGAGACAGAGAGAGAGAAAGAGAGAGAAGAGGAGGAAGAGAGGGAGGCAGAGAGAGAGGAGGAGGAAGCTAGGGAGAGAGAGGAAGAACATCAAAGAGAGAGAGGGAAGAGGCGGAGAGAGGGGGAGGAAAGACAAAGAAGAGGGTGGGAGGAAGAAGAAGAAGAAGAAGAAGGTGGAGGAGAGGAGCCGCAGCTGCCAGTACTAAGAATTTTGGAACAATGGACTTGA

Coding sequence (CDS)

ATGAAGAAACGCACAGCAGTTTCAGTTTCAGGATCCTCGTTTTCACCTTCCATTCTTATCCCTATCTTCTTCCTCTTCCTCTCTCTTCCTGCATATGCCGATGACGGGTGGTGGGAAGGAGACAGTCCTGTGGTGAAGAGAGCCAATGAAAGAATCCCAATTCTTAAAACAGAGTACGGTGAGATCTCCGCGGTTGATTTCGACGATGGCACTCGATTTGGACGTTACCATCTTCAGTTCATCACATTGGAACCCAATTCACTGTTTCTCCCTGTTCTTCTTCATTCTGATATGGTGTTCTATGTCCACACTGGAAGTGGGAGATTGAATTGGTTTGATGACAATGGTTTGAAGGAGGTGGATTTACGGCGGGGAGATCTTTATAGGCTTCATCCAGGCTCCATTTTTTACTTGCAGAGTAGCTTTGAGACCGAACGTGAAAAGCTTCGAATTTATGCTCTGTTTTCCAGCACAGATGAGGATTCATTCAATCCTTCCATTGGAGCATACTCCCGCGTCACTGATCTGGTTCGTGGCTTCGGCAAAGAAGTTCTTCGTAAAGCCTTCATGGCCCCTGATGAAGTAATAGAGGAAATAATGAACACTAAGAGGCCGCCGCTTATCGTCCACGCTGCTGCGCCAACTCCGAGCATAAAGGCAAAGTCGTCGTCACCATGGGAATTTGAGGCTCGGTTATTGAAAACTTTCCTAGGAGGAGACGCAAGTGCGACAGAATTCAACAAGAAGAAGAAGAAGAAAGGCATATACAACGTTTATGAAGTAGACCCTGATTTTGAGAATTGCAATGGATGGAGTTTGACTGTAACTAAGAAAAACTCCCATCAATTGAAAGGCTCCAACATCGGCTTCCTCGTAGTCAACCTTACAGCGGGATCAATGATGGGTCCGCATTGGAATCCGAGGGCGTGGGAGATTGGGATTGTGACATCGGACGAGCCGGGGGTGGTTCGTGTAGGGTGTTCGAGCACCTCGGCAAACAGTTCAAAATGCAAGAATTGGAGTTTTGTGGTAGAGAAAGGGGATGTATTTGTAGTGCCAAGGTTCCATCCAATGGCGCAAATGTCATTCAACAATGGAACGTTTGTATTTGTGGGATTTAGCACAACGAATGGACATAACATGCCGCAGTTCTTTGCTGGGAGTAGCTCTGTTTTGAAAATTGTGGACAGGGAAGTATTGGCATGGTCGTTTGATGTGAATGTGACAACGATTGATCGGTTGTTGAAAGCTAGAGTTGAGTCGATTGTTTTGGAATGTACTTCATGTGCTGAAGAGGAAGTAAGGAAAATGGAAGAGGAAGCTGAGAGAGAGAGGGAGGAGGAGGAAGAAAGAAAGAGAGAAGAGGAGGAAGAAGAGAGAAAAAGGGAAGAAGAGGAACGGAGGAAGAGAGAGGAAGAGGAAGAGAGGAAACGGGAAGAAGAAGAAGAGAGAAAAAGGGAAGAAGAGGAAGAGGAAGAGAGGAAACGGGAAGAAGAAGAAGAGAGAAAAAGGGAAGAAGAGGAAGAGGAAGAGAGGAAACGGGAAGAAGAAGAAGAGAGAAAAAGGGAAGAAGAGGAACGGAGGAAGAGAGAGGAAGAGGAAGAGAGGGAGAGAGAAGAAGAAGAAGCACAGAAAGAGGAAGAGTGGAGGAGAGAAGAGGAAGAGAGGAGAAAGAGAGAAGAGGAAGAGAGAAAAAGAGAGGAAGAGGAGACAGAGAGAGAGAAAGAGAGAGAAGAGGAGGAAGAGAGGGAGGCAGAGAGAGAGGAGGAGGAAGCTAGGGAGAGAGAGGAAGAACATCAAAGAGAGAGAGGGAAGAGGCGGAGAGAGGGGGAGGAAAGACAAAGAAGAGGGTGGGAGGAAGAAGAAGAAGAAGAAGAAGGTGGAGGAGAGGAGCCGCAGCTGCCAGTACTAAGAATTTTGGAACAATGGACTTGA

Protein sequence

MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYGEISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEVDLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKTFLGGDASATEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEEEERKREEEERRKREEEEERKREEEEERKREEEEEEERKREEEEERKREEEEEEERKREEEEERKREEEERRKREEEEEREREEEEAQKEEEWRREEEERRKREEEERKREEEETEREKEREEEEEREAEREEEEAREREEEHQRERGKRRREGEERQRRGWEEEEEEEEGGGEEPQLPVLRILEQWT*
Homology
BLAST of Chy2G029310 vs. ExPASy Swiss-Prot
Match: F4IQK5 (Vicilin-like seed storage protein At2g18540 OS=Arabidopsis thaliana OX=3702 GN=At2g18540 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 5.8e-94
Identity = 309/619 (49.92%), Postives = 410/619 (66.24%), Query Frame = 0

Query: 42  SPVVKRANERIPILKTEYGEISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFY 101
           SP++ + ++R  ++ TE+G ISAV   DG     YH+QFITLEPN+L LP+LLHSDMVF+
Sbjct: 41  SPLLVKKDQRTSVVATEFGNISAVQIGDG-----YHIQFITLEPNALLLPLLLHSDMVFF 100

Query: 102 VHTGSGRLNWFDDNGLKEVDLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDED 161
           VHTG+G LNW D+   ++++LRRGD++RL  G++FY+ S+     EKLR+YA+F +  + 
Sbjct: 101 VHTGTGILNWIDEESERKLELRRGDVFRLRSGTVFYVHSN-----EKLRVYAIF-NVGKC 160

Query: 162 SFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAK 221
             +P +GAYS V DL+ GF    LR AF  P++++ +I +  +PPLIV+A    P  + +
Sbjct: 161 LNDPCLGAYSSVRDLLLGFDDRTLRSAFAVPEDILRKIRDATKPPLIVNA---LPRNRTQ 220

Query: 222 SSSPWEFEARLLKTFLGGD------ASATEFNKKKKKKGIYNVYEVDPDFENCNGWSLTV 281
                ++++RL++ F+  +      A     +  KKK   +NV+E DPDFEN NG S+ V
Sbjct: 221 GLEEDKWQSRLVRLFVSVEDVTDHLAMKPIVDTNKKKSRTFNVFEEDPDFENNNGRSIVV 280

Query: 282 TKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSS 341
            +K+   LKGS  G  +VNLT GSM+GPHWNP A EI IV   E  V  V   S S+  +
Sbjct: 281 DEKDLDALKGSRFGVFMVNLTKGSMIGPHWNPSACEISIVLEGEGMVRVVNQQSLSSCKN 340

Query: 342 KCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIV 401
             K+ SF+VE+GDVFVVP+FHPMAQMSF N +FVF+GFST+   N PQF  G SSVLK++
Sbjct: 341 DRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFSTSAKTNHPQFLVGQSSVLKVL 400

Query: 402 DREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKME---EEAEREREEEEERK 461
           DR+V+A SF+++  TI  LLKA+ ES++ EC SCAE E+ K+    EE +R  EEE ER+
Sbjct: 401 DRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELSKLMREIEERKRREEEEIERR 460

Query: 462 REEEEEERKREEEERR-----KREEEEE---RKREEEEERKREEE---EEEERKREEEEE 521
           R+EEEE RKREE +RR     KR EEEE   +KREEEE RKREEE   EEEE KR EEE 
Sbjct: 461 RKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRREEER 520

Query: 522 RKREEEEEEERKREEEEE--------------RKREEEERRKREEEEEREREEEEAQKEE 581
           +KREEE E+ RKREEE E              RK  EE  RKR EE+ER+R EEEA+K E
Sbjct: 521 KKREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKRREEQERKRREEEARKRE 580

Query: 582 EWRREEEERRKREEEERKREEEETEREKEREEEEEREAEREEEEAREREEEHQRERGKRR 627
           E R+ EEE  KR E+ER+R+E E   E ER+  EE+E +REEE A+ RE+E Q    K+ 
Sbjct: 581 EERKREEEMAKRREQERQRKERE---EVERKIREEQERKREEEMAKRREQERQ----KKE 638

BLAST of Chy2G029310 vs. ExPASy Swiss-Prot
Match: F4JQG6 (Vicilin-like seed storage protein At4g36700 OS=Arabidopsis thaliana OX=3702 GN=At4g36700 PE=3 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.9e-89
Identity = 238/491 (48.47%), Postives = 329/491 (67.01%), Query Frame = 0

Query: 42  SPVVKRANERIPILKTEYGEISAVDFDDGT-RFGRYHLQFITLEPNSLFLPVLLHSDMVF 101
           SP++ + ++  PI +T++G+IS V   +G    G Y +  ITLEPN++ LP+LLHSDMVF
Sbjct: 45  SPLLIKKDQWKPIFETKFGQISTVQIGNGCGGMGPYKIHSITLEPNTILLPLLLHSDMVF 104

Query: 102 YVHTGSGRLNWFDDNGLKEVDLRRGDLYRLHPGSIFYLQSSFET--EREKLRIYALFSST 161
           +V +GSG LNW D+   K  ++R GD+YRL PGS+FYLQS         KL++YA+FS+ 
Sbjct: 105 FVDSGSGILNWVDEEA-KSTEIRLGDVYRLRPGSVFYLQSKPVDIFLGTKLKLYAIFSNN 164

Query: 162 DEDSFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSI 221
           DE   +P  GAYS +TDL+ GF + +L+ AF  P+ +IE + N  +PPLIV     TP +
Sbjct: 165 DECLHDPCFGAYSSITDLMFGFDETILQSAFGVPEGIIELMRNRTKPPLIVSETLCTPGV 224

Query: 222 KAKSSSPWEFEARLLKTFLGGDASATEFNKKKKKK---------GIYNVYEVDPDFENCN 281
               ++ W+ + RLLK F  G A   +  KKK+KK           +NV+E +PDFE+  
Sbjct: 225 ----ANTWQLQPRLLKLF-AGSADLVDNKKKKEKKEKKEKVKKAKTFNVFESEPDFESPY 284

Query: 282 GWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVVRVGCSS 341
           G ++T+ +K+   LKGS +G  +VNLT GSMMGPHWNP A EI IV     G+VRV  SS
Sbjct: 285 GRTITINRKDLKVLKGSMVGVSMVNLTQGSMMGPHWNPWACEISIVLKG-AGMVRVLRSS 344

Query: 342 TSAN-SSKCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGS 401
            S+N SS+CKN  F VE+GD+F VPR HPMAQMSFNN + VFVGF+T+  +N PQF AG 
Sbjct: 345 ISSNTSSECKNVRFKVEEGDIFAVPRLHPMAQMSFNNDSLVFVGFTTSAKNNEPQFLAGE 404

Query: 402 SSVLKIVDREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKMEEEAEREREEE 461
            S L+++DR+VLA S +V+  TID LL A+ E+++LEC SCAE E+ K++ E ER++ ++
Sbjct: 405 DSALRMLDRQVLAASLNVSSVTIDGLLGAQKEAVILECHSCAEGEIEKLKVEIERKKIDD 464

Query: 462 EERKREEEEEERKREEEERRKREEEEERKREEEEERKREEEEEEERKREEEEERKREEEE 520
           E ++R    +ERK+EEEE  KREEEE RKREEEEE+KR   ++     +EEE R+R+   
Sbjct: 465 ERKRR---HDERKKEEEE-AKREEEERRKREEEEEKKRWPPQQPP---QEEELRERQLPM 521

BLAST of Chy2G029310 vs. ExPASy Swiss-Prot
Match: Q9SK09 (Vicilin-like seed storage protein At2g28490 OS=Arabidopsis thaliana OX=3702 GN=At2g28490 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 2.6e-38
Identity = 118/422 (27.96%), Postives = 200/422 (47.39%), Query Frame = 0

Query: 54  ILKTEYGEISAVDFDDGTRFGR-YHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWF 113
           ++K+E GE+  V    G    +  H+ F+T+EP +LF+P  L S ++ ++  G   L   
Sbjct: 94  VIKSEGGEMRVVLSPRGRIIEKPMHIGFLTMEPKTLFVPQYLDSSLLIFIRQGEATLGVI 153

Query: 114 DDNGLKEVDLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAY-- 173
             +   E  L+ GD+Y +  GS+FYL ++   +R  L +      T    F      Y  
Sbjct: 154 CKDEFGERKLKAGDIYWIPAGSVFYLHNTGLGQR--LHVICSIDPTQSLGFETFQPFYIG 213

Query: 174 SRVTDLVRGFGKEVLRKAF-MAPDEVIEEIMNTKRPPLIVHAAAPTPS------------ 233
              + ++ GF    L  AF ++  E+ + +M+  R P++     P P             
Sbjct: 214 GGPSSVLAGFDPHTLTSAFNVSLPELQQMMMSQFRGPIVYVTEGPQPQPQSTVWTQFLGL 273

Query: 234 ------------IKAKSSSP--------WEFEARLLKTFLGGDASATEFNKKKKKKGIYN 293
                       ++ K  SP        W +   ++++ L       + +   + +  YN
Sbjct: 274 RGEEKHKQLKKLLETKQGSPQDQQYSSGWSWR-NIVRSILDLTEEKNKGSGSSECEDSYN 333

Query: 294 VYEV--DPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIV 353
           +Y+    P F+N  GWS+ +   +   LK S IG  +VNLTAG+MM PH NP A E GIV
Sbjct: 334 IYDKKDKPSFDNKYGWSIALDYDDYKPLKHSGIGVYLVNLTAGAMMAPHMNPTATEYGIV 393

Query: 354 TSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFST 413
            +    +  V  + TSA +++       V  GDVF +PR+    Q++   G F FVGF+T
Sbjct: 394 LAGSGEIQVVFPNGTSAMNTR-------VSVGDVFWIPRYFAFCQIASRTGPFEFVGFTT 453

Query: 414 TNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVR 438
           +   N PQF  GS+S+L+ ++   L+ +F V+  T+ R ++A+ E+++L   + A   V 
Sbjct: 454 SAHKNRPQFLVGSNSLLRTLNLTSLSIAFGVDEETMRRFIEAQREAVILPTPAAAPPHVG 505

BLAST of Chy2G029310 vs. ExPASy TrEMBL
Match: A0A5D3CNA8 (Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002250 PE=4 SV=1)

HSP 1 Score: 917.1 bits (2369), Expect = 4.0e-263
Identity = 568/674 (84.27%), Postives = 601/674 (89.17%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK TA+SVSGS FS S LI IFFLF SLPAYADDGWWEGDSPVVKRANERI +LKTEYG
Sbjct: 1   MKKPTAISVSGSPFSLSFLISIFFLFFSLPAYADDGWWEGDSPVVKRANERIQLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           +ISAVDFDDG+RFG YHLQFITLEPNSLFLPVLLHSDMVFY+HTGSGRLNWFD+N LKEV
Sbjct: 61  DISAVDFDDGSRFGPYHLQFITLEPNSLFLPVLLHSDMVFYIHTGSGRLNWFDENDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSS E EREKLRIYALFSSTDEDSFNPS+GAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLEIEREKLRIYALFSSTDEDSFNPSLGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKTFLGGD 240
           GKEVLRKAFMAPDEVIEEIM  KRPPLIVHAAAPTPSI+AKSSSPWEFEARLLK FLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMTAKRPPLIVHAAAPTPSIRAKSSSPWEFEARLLKAFLGGD 240

Query: 241 ASATEFN-KKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300
           AS  EFN KKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS
Sbjct: 241 ASGIEFNKKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300

Query: 301 MMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMA 360
           MMGPHWNPRAWEIGIVTSDEPGVV VGCSSTSANSSKCKNWSFVVEKGD+FVVPRFHPMA
Sbjct: 301 MMGPHWNPRAWEIGIVTSDEPGVVHVGCSSTSANSSKCKNWSFVVEKGDIFVVPRFHPMA 360

Query: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARV 420
           QMSFNNGTFVFVGFSTTNGHNMPQFF GSSSVL++VDREVLAWSFDVNVTT+DRLLKARV
Sbjct: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFVGSSSVLQLVDREVLAWSFDVNVTTVDRLLKARV 420

Query: 421 ESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEEEERKREEEERRKREEEEERKRE 480
           ESI+LECTSCAEEEVRKMEEEAEREREEEEERKR EEEE+RKREEEE+RKREEEE RKRE
Sbjct: 421 ESIILECTSCAEEEVRKMEEEAEREREEEEERKR-EEEEQRKREEEEQRKREEEERRKRE 480

Query: 481 EEEERKREE------EEEEERKREEEEERKREEE---EEEERKREEEEERKREEEERRKR 540
           EEE+RKREE      EEEE RKREEEEE +REEE   EEEE +REEE  R+REEEE+R++
Sbjct: 481 EEEQRKREEEEQRKREEEERRKREEEEEAEREEERKREEEEAQREEERRRRREEEEKREK 540

Query: 541 EEEEERER---EEEEAQKEEEWRREEEERRKREEEERKREEEETEREKEREE-------- 600
           E EEE +R   EEE+ Q+EEE +REEE RR+REEEERKREEEE E+E+E E+        
Sbjct: 541 EREEEEQRRREEEEQQQEEEEAQREEERRRRREEEERKREEEEGEKEREEEQQQEEEEAK 600

Query: 601 ---------EEEREAEREEEEAREREEEHQRERGKRRREGEERQRRGWEEEEEEEEGGGE 645
                    EEEREA+REEEEAREREEEHQRERG+RRRE EE QRR W  EEEE EG  E
Sbjct: 601 REEEEERKREEEREAKREEEEAREREEEHQRERGRRRREAEEGQRRRW--EEEEGEGEEE 660

BLAST of Chy2G029310 vs. ExPASy TrEMBL
Match: A0A0A0KGF2 (PreproMP73 OS=Cucumis sativus OX=3659 GN=Csa_6G502040 PE=4 SV=1)

HSP 1 Score: 902.1 bits (2330), Expect = 1.3e-258
Identity = 563/650 (86.62%), Postives = 592/650 (91.08%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK +AVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGD+PVVKRANERIPILKTEYG
Sbjct: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDN LKEV
Sbjct: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSS ETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKTFLGGD 240
           GKEVLRKAFMAPDEVIEEIMN KRPPLIVHAAAPTPSIKAKSSSPWEFEARLLK+FLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD 240

Query: 241 ASATEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300
           ASA EFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM
Sbjct: 241 ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300

Query: 301 MGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360
           MGPHWNPRAWEIGIVTSDEPGV+RVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ
Sbjct: 301 MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360

Query: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420
           MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE
Sbjct: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420

Query: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKRE-------EEEEERKREEEERRKREEE 480
           SIVLECTSCAEEEVRKMEEEAEREREEEEERKRE       EEEEERKREEEERRKREEE
Sbjct: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEERRKREEEEERKREEEERRKREEE 480

Query: 481 EERKREE---EEERKR-EEEEEEERKREEEEERKR-EEEEEEERKREEEEERKREEEERR 540
           EERKREE   EEERKR EEEEEEERKREEEEE+KR EEEEEEERKREEEEE+KREEE   
Sbjct: 481 EERKREEEEREEERKREEEEEEEERKREEEEEKKREEEEEEEERKREEEEEKKREEE--- 540

Query: 541 KREEEEEREREEEEAQKEEEWRREEEERRKREE---EERKREEEETEREKEREEEEER-- 600
             EEEEER+REEEE +K EE   EEE +RKR+    E+RKRE+E   RE+ER+ EE R  
Sbjct: 541 --EEEEERKREEEEEKKREEEEEEEERKRKRKRGGGEKRKREKERKRRERERKREERRNR 600

Query: 601 --EAEREEEEAREREEEHQRERGKRRREGEERQRRGWEEEEEEEEGGGEE 632
             E ++ +++ R R+ E +R+ GK R+  +ER+ RG E   +++E GG +
Sbjct: 601 GGEKKKRKKKKRGRQRERRRKLGKERKNIKEREGRGGERGRKDKEDGGRK 645

BLAST of Chy2G029310 vs. ExPASy TrEMBL
Match: A0A1S3B2F9 (vicilin-like seed storage protein At2g18540 OS=Cucumis melo OX=3656 GN=LOC103485029 PE=4 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.8e-258
Identity = 551/658 (83.74%), Postives = 592/658 (89.97%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK TA+SVSGS FS S LI IFFLF SLPAYADDGWWEGDSPVVKRANERI +LKTEYG
Sbjct: 1   MKKPTAISVSGSPFSLSFLISIFFLFFSLPAYADDGWWEGDSPVVKRANERIQLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           +ISAVDFDDG+RFG YHLQFITLEPNSLFLPVLLHSDMVFY+HTGSGRLNWFD+N LKEV
Sbjct: 61  DISAVDFDDGSRFGPYHLQFITLEPNSLFLPVLLHSDMVFYIHTGSGRLNWFDENDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSS E EREKLRIYALFSSTDEDSFNPS+GAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLEIEREKLRIYALFSSTDEDSFNPSLGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKTFLGGD 240
           GKEVLRKAFMAPDEVIEEIM  KRPPLIVHAAAPTPSI+AKSSSPWEFEARLLK FLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMTAKRPPLIVHAAAPTPSIRAKSSSPWEFEARLLKAFLGGD 240

Query: 241 ASATEFN-KKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300
           AS  EFN KKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS
Sbjct: 241 ASGIEFNKKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300

Query: 301 MMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMA 360
           MMGPHWNPRAWEIGIVTSDEPGVV VGCSSTSANSSKCKNWSFVVEKGD+FVVPRFHPMA
Sbjct: 301 MMGPHWNPRAWEIGIVTSDEPGVVHVGCSSTSANSSKCKNWSFVVEKGDIFVVPRFHPMA 360

Query: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARV 420
           QMSFNNGTFVFVGFSTTNGHNMPQFF GSSSVL++VDREVLAWSFDVNVTT+DRLLKARV
Sbjct: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFVGSSSVLQLVDREVLAWSFDVNVTTVDRLLKARV 420

Query: 421 ESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEEEERKREEEERR--------KRE 480
           ESI+LECTSCAEEEVRKMEEEAEREREEEEERKREEEE  ++ EEE+R+        KRE
Sbjct: 421 ESIILECTSCAEEEVRKMEEEAEREREEEEERKREEEERRKREEEEQRKRXXXXXXXKRE 480

Query: 481 EEEERKREEEEERKREEE---EEEERKREEEEERKREEEEEEERKREEEEERKREEEERR 540
           EEE RKREEEEE +REEE   EEEE +REEE  R+REEEE+ E++REEEE+R+REEEE++
Sbjct: 481 EEERRKREEEEEAEREEERKREEEEAQREEERRRRREEEEKREKEREEEEQRRREEEEQQ 540

Query: 541 KREEEEEREREEEEAQKEEEWRREEEERRKREEEERKREEEETER--EKEREEEEEREAE 600
           + EEE +RE E    ++EEE +REEEE  K  EEE+++EEEE +R  E+ER+ EEEREA+
Sbjct: 541 QEEEEAQREEERRRRREEEERKREEEEGEKEREEEQQQEEEEAKREEEEERKREEEREAK 600

Query: 601 REEEEAREREEEHQRERGKRRREGEERQRRGWEEEEEEEEGGGEEPQLPVLRILEQWT 645
           REEEEAREREEEHQRERG+RRRE EE QRR W  EEEE EG  EE + PVLRIL QWT
Sbjct: 601 REEEEAREREEEHQRERGRRRREAEEGQRRRW--EEEEGEGEEEEEEQPVLRILSQWT 656

BLAST of Chy2G029310 vs. ExPASy TrEMBL
Match: A0A6J1GFV7 (vicilin-like seed storage protein At2g18540 OS=Cucurbita moschata OX=3662 GN=LOC111453792 PE=4 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 2.9e-205
Identity = 471/651 (72.35%), Postives = 517/651 (79.42%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK TA  +SGS FS S L  +FFLF+SLP+ ADD WWEG  PVVKRANER  +LKTEYG
Sbjct: 1   MKKSTA--ISGSPFSLSFLFTVFFLFVSLPSNADDKWWEGACPVVKRANERRSLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           EISA+D  D ++FG YHLQFIT+EPNSLFLPVLLH+DMV Y+HTGSGRLNWFDD+ L+EV
Sbjct: 61  EISAIDLHDASQFGPYHLQFITMEPNSLFLPVLLHADMVLYMHTGSGRLNWFDDDDLREV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGD++RL PG+IFY+ SS ETEREKLR+YALFSSTDED F P+IGAYSRVTD VRGF
Sbjct: 121 DLRRGDIFRLQPGAIFYIHSSLETEREKLRMYALFSSTDEDPFEPAIGAYSRVTDHVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAA-----PTPSIKAKSSSPWEFEARLLKT 240
            KEVL KAFM P+EVIEEIM+ KRPPLIVHAA      PT S+    S   E EAR LK+
Sbjct: 181 DKEVLCKAFMVPEEVIEEIMDAKRPPLIVHAATTLSKKPTSSL----SMSLELEARFLKS 240

Query: 241 FLGGDASATEFN-KKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVN 300
           F+GG  S  +FN KKKKKKG+YNV+E DPDFENCNGWSLTVTKK SHQLKGSNIGF VVN
Sbjct: 241 FIGGGGSGMDFNKKKKKKKGLYNVFEADPDFENCNGWSLTVTKKVSHQLKGSNIGFFVVN 300

Query: 301 LTAGSMMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPR 360
           LTAGSMMGPHWNPRAWEIGIVTS+E GVVRVGCSS + NSS CK WSFVV KGDVFVVPR
Sbjct: 301 LTAGSMMGPHWNPRAWEIGIVTSEEAGVVRVGCSSMT-NSSICKKWSFVVGKGDVFVVPR 360

Query: 361 FHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRL 420
           FHPMAQMSFNNG+FVFVGFSTTN +N+PQF AG SSVL+ VDREVLAWSFDVNVTTIDRL
Sbjct: 361 FHPMAQMSFNNGSFVFVGFSTTNRNNLPQFLAGRSSVLQTVDREVLAWSFDVNVTTIDRL 420

Query: 421 LKARVESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEEEERKREEEERRKREEEE 480
           L ARVES++LECTSCAEEEVRKM EEAERER+EEEERKREE                   
Sbjct: 421 LGARVESVILECTSCAEEEVRKMVEEAERERQEEEERKREE------------------- 480

Query: 481 ERKREEEEERKREEEEEEERKREEEEERKREEEEEEERKREEEEERKREEEERRKREEEE 540
               EEEEERKR+EEEEEERKR+EEE ++     EEER+R EEEER+REEEE RKR EEE
Sbjct: 481 ----EEEEERKRKEEEEEERKRKEEEAKR-----EEERRRREEEEREREEEEARKR-EEE 540

Query: 541 EREREEEEAQKEEEWRREEEERRKR-EEEERKREEEETEREKEREEEEEREAEREEEEAR 600
           EREREEE     E  RREEEE RKR EEEER+REEEE  RE+E     EREAERE EEAR
Sbjct: 541 EREREEE----AERGRREEEEARKREEEEEREREEEEARREEEEAVRGEREAEREAEEAR 600

Query: 601 EREEEHQRERGKRRREGEERQRRGWEEEEEEEEGGGEEPQLPVLRILEQWT 645
           E EE H+RERG+RRRE EERQRR  EEEEE           P LRIL Q T
Sbjct: 601 ESEEAHRRERGRRRREAEERQRRRREEEEE-----------PTLRILRQRT 600

BLAST of Chy2G029310 vs. ExPASy TrEMBL
Match: A0A6J1IU23 (vicilin-like seed storage protein At2g18540 OS=Cucurbita maxima OX=3661 GN=LOC111478419 PE=4 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 1.6e-203
Identity = 489/671 (72.88%), Postives = 541/671 (80.63%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK TA  +SGS FS S L  +FFLFLSLP+ ADD WWE   P VKRANER  +LKTEYG
Sbjct: 1   MKKSTA--ISGSPFSLSFLFTVFFLFLSLPSNADDKWWEAACP-VKRANERKSLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           EISAVD  D ++FG YHLQFIT+EPNSLFLPVLLH+DMV Y+HTGSGRLNWFDD+ L+EV
Sbjct: 61  EISAVDLHDASQFGPYHLQFITMEPNSLFLPVLLHADMVLYMHTGSGRLNWFDDDDLREV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGD++RL PG+IFY+ SS ETEREKLR+YALFSSTDED F P+IGAYSRVTD VRGF
Sbjct: 121 DLRRGDIFRLQPGAIFYIHSSLETEREKLRMYALFSSTDEDPFEPAIGAYSRVTDHVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAKS----SSPWEFEARLLKTF 240
            KE+L KAFM P+EVIEEIM+ KRPPLIVHAA    ++  K     S   E EAR LK+F
Sbjct: 181 DKEILCKAFMVPEEVIEEIMDAKRPPLIVHAATTLSTLSKKQRSSLSMSLELEARFLKSF 240

Query: 241 LGGDASATEF--NKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVN 300
           +GG     +F   KKKKKKG+YNV+E DPDFENCNGWSLTVTKK SHQLKGSNIGF VVN
Sbjct: 241 IGGGGIGMDFKKKKKKKKKGLYNVFEADPDFENCNGWSLTVTKKVSHQLKGSNIGFFVVN 300

Query: 301 LTAGSMMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPR 360
           LTAGSMMGPHWNPRAWEIGIVTS+E GVVRVGCSS + NSSKCK WSFVV KGDVFVVPR
Sbjct: 301 LTAGSMMGPHWNPRAWEIGIVTSEEAGVVRVGCSSMT-NSSKCKKWSFVVGKGDVFVVPR 360

Query: 361 FHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRL 420
           FHPMAQMSFNNG+F FVGFSTTN +N+PQF AG SSVL+ V+R+VLAWSFDVNVTTIDRL
Sbjct: 361 FHPMAQMSFNNGSFAFVGFSTTNRNNLPQFLAGRSSVLQTVERQVLAWSFDVNVTTIDRL 420

Query: 421 LKARVESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEEEERKREEEERRKREEEE 480
           L+ARVES++LECTSCAEEEV KMEEEAERER+EEEER+R  EEEER+REEEE RKR EEE
Sbjct: 421 LEARVESVILECTSCAEEEVMKMEEEAERERQEEEERRR--EEEEREREEEEARKR-EEE 480

Query: 481 ERKREEEEERKREEEEEEERKREEEEERKREEEEEEERKREEEEERKREEEERRKREEEE 540
           ER+REEEE RKR   EEEER+REEEEER+R   EEEER+REEEE RKREEEE RKR EEE
Sbjct: 481 EREREEEEARKR---EEEEREREEEEERRR---EEEEREREEEEARKREEEEARKR-EEE 540

Query: 541 EREREEEEAQK--EEEWRREEEERRKREEEE-RKREEEETEREKE-----REEEE----- 600
           EREREEEEA+K  EEE  REEEE RKRE+EE RKREEEE ERE+E     REEEE     
Sbjct: 541 EREREEEEARKREEEEREREEEEARKREKEEARKREEEEREREEEAERERREEEEEARRR 600

Query: 601 ------EREAEREEEEAREREEEHQRERGKRRREGEE--RQRRGWEEEEEEEEGGGEEPQ 645
                 EREAERE EEARE EE H+RERG+RRRE E    +R+G   EEEEE        
Sbjct: 601 EEAERGEREAEREAEEARESEEAHRRERGRRRREAEREAEERQGRRREEEEE-------- 648

BLAST of Chy2G029310 vs. NCBI nr
Match: XP_011658490.2 (vicilin-like seed storage protein At2g18540 [Cucumis sativus] >KAE8647587.1 hypothetical protein Csa_003555 [Cucumis sativus])

HSP 1 Score: 1011 bits (2613), Expect = 0.0
Identity = 621/676 (91.86%), Postives = 629/676 (93.05%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK +AVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGD+PVVKRANERIPILKTEYG
Sbjct: 1   MKKHSAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDTPVVKRANERIPILKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDN LKEV
Sbjct: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSS ETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKTFLGGD 240
           GKEVLRKAFMAPDEVIEEIMN KRPPLIVHAAAPTPSIKAKSSSPWEFEARLLK+FLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMNAKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKSFLGGD 240

Query: 241 ASATEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300
           ASA EFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM
Sbjct: 241 ASAIEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSM 300

Query: 301 MGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360
           MGPHWNPRAWEIGIVTSDEPGV+RVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ
Sbjct: 301 MGPHWNPRAWEIGIVTSDEPGVIRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQ 360

Query: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420
           MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE
Sbjct: 361 MSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVE 420

Query: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE-------EERKREEEERRKREEE 480
           SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEE       EERKREEEERRKREEE
Sbjct: 421 SIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEERRKREEEEERKREEEERRKREEE 480

Query: 481 EERKREEEEERKREEEEEEERKREEEEERKREEEEEEERKREEEEERKREEEERRKREEE 540
           EERKREEEE RKREEEEE  +KREEEEERKREEEEE  +KREEEEERKREEEERRKREEE
Sbjct: 481 EERKREEEERRKREEEEE--KKREEEEERKREEEEE--KKREEEEERKREEEERRKREEE 540

Query: 541 EER-----------EREEEEAQKEEEWRREEEERRKREEEERKREEEETEREKER----- 600
           EER           EREEEEAQKEEE RREEEERR+REEEERKREEEE EREKER     
Sbjct: 541 EERKREEEEEEEEREREEEEAQKEEERRREEEERRRREEEERKREEEEREREKERGEEEQ 600

Query: 601 -------EEEEEREAEREEEEAREREEEHQRERGKRRREGEERQRRGWEEEEEEEEGGGE 644
                  EEEEEREAEREEEEAR+REEEHQRERGKRRREGEERQRR WEEEEEE  GGGE
Sbjct: 601 RRREEEEEEEEEREAEREEEEARKREEEHQRERGKRRREGEERQRRRWEEEEEE--GGGE 660

BLAST of Chy2G029310 vs. NCBI nr
Match: TYK12638.1 (vicilin-like seed storage protein [Cucumis melo var. makuwa])

HSP 1 Score: 918 bits (2372), Expect = 0.0
Identity = 571/677 (84.34%), Postives = 602/677 (88.92%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK TA+SVSGS FS S LI IFFLF SLPAYADDGWWEGDSPVVKRANERI +LKTEYG
Sbjct: 1   MKKPTAISVSGSPFSLSFLISIFFLFFSLPAYADDGWWEGDSPVVKRANERIQLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           +ISAVDFDDG+RFG YHLQFITLEPNSLFLPVLLHSDMVFY+HTGSGRLNWFD+N LKEV
Sbjct: 61  DISAVDFDDGSRFGPYHLQFITLEPNSLFLPVLLHSDMVFYIHTGSGRLNWFDENDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSS E EREKLRIYALFSSTDEDSFNPS+GAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLEIEREKLRIYALFSSTDEDSFNPSLGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKTFLGGD 240
           GKEVLRKAFMAPDEVIEEIM  KRPPLIVHAAAPTPSI+AKSSSPWEFEARLLK FLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMTAKRPPLIVHAAAPTPSIRAKSSSPWEFEARLLKAFLGGD 240

Query: 241 ASATEFNKKKKKK-GIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300
           AS  EFNKKKKKK GIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS
Sbjct: 241 ASGIEFNKKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300

Query: 301 MMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMA 360
           MMGPHWNPRAWEIGIVTSDEPGVV VGCSSTSANSSKCKNWSFVVEKGD+FVVPRFHPMA
Sbjct: 301 MMGPHWNPRAWEIGIVTSDEPGVVHVGCSSTSANSSKCKNWSFVVEKGDIFVVPRFHPMA 360

Query: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARV 420
           QMSFNNGTFVFVGFSTTNGHNMPQFF GSSSVL++VDREVLAWSFDVNVTT+DRLLKARV
Sbjct: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFVGSSSVLQLVDREVLAWSFDVNVTTVDRLLKARV 420

Query: 421 ESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEEEERKREEEERRKREEEEERKRE 480
           ESI+LECTSCAEEEVRKMEEEAEREREEEEERKREEEE+ RKREEEE+RKREEEE RKRE
Sbjct: 421 ESIILECTSCAEEEVRKMEEEAEREREEEEERKREEEEQ-RKREEEEQRKREEEERRKRE 480

Query: 481 EEEERKREEEEEEERKREEEEERKREEEEE----EERKREEEEERKREEEERRKREEEEE 540
           EEE+RKREEEE+  RKREEEE RKREEEEE    EERKREEEE  +REEE RR+REEEE+
Sbjct: 481 EEEQRKREEEEQ--RKREEEERRKREEEEEAEREEERKREEEE-AQREEERRRRREEEEK 540

Query: 541 RERE-----------EEEAQKEEEWRREEEERRKREEEERKREEEETEREKEREE----- 600
           RE+E           EE+ Q+EEE +REEE RR+REEEERKREEEE E+E+E E+     
Sbjct: 541 REKEREEEEQRRREEEEQQQEEEEAQREEERRRRREEEERKREEEEGEKEREEEQQQEEE 600

Query: 601 ------------EEEREAEREEEEAREREEEHQRERGKRRREGEERQRRGWEEEEEEEEG 644
                       EEEREA+REEEEAREREEEHQRERG+RRRE EE QRR WEEEE E  G
Sbjct: 601 EAKREEEEERKREEEREAKREEEEAREREEEHQRERGRRRREAEEGQRRRWEEEEGE--G 660

BLAST of Chy2G029310 vs. NCBI nr
Match: XP_008440688.1 (PREDICTED: vicilin-like seed storage protein At2g18540 [Cucumis melo])

HSP 1 Score: 901 bits (2329), Expect = 0.0
Identity = 560/674 (83.09%), Postives = 588/674 (87.24%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK TA+SVSGS FS S LI IFFLF SLPAYADDGWWEGDSPVVKRANERI +LKTEYG
Sbjct: 1   MKKPTAISVSGSPFSLSFLISIFFLFFSLPAYADDGWWEGDSPVVKRANERIQLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           +ISAVDFDDG+RFG YHLQFITLEPNSLFLPVLLHSDMVFY+HTGSGRLNWFD+N LKEV
Sbjct: 61  DISAVDFDDGSRFGPYHLQFITLEPNSLFLPVLLHSDMVFYIHTGSGRLNWFDENDLKEV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGDLYRLHPGSIFYLQSS E EREKLRIYALFSSTDEDSFNPS+GAYSRVTDLVRGF
Sbjct: 121 DLRRGDLYRLHPGSIFYLQSSLEIEREKLRIYALFSSTDEDSFNPSLGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAKSSSPWEFEARLLKTFLGGD 240
           GKEVLRKAFMAPDEVIEEIM  KRPPLIVHAAAPTPSI+AKSSSPWEFEARLLK FLGGD
Sbjct: 181 GKEVLRKAFMAPDEVIEEIMTAKRPPLIVHAAAPTPSIRAKSSSPWEFEARLLKAFLGGD 240

Query: 241 ASATEFNKKKKKK-GIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300
           AS  EFNKKKKKK GIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS
Sbjct: 241 ASGIEFNKKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGS 300

Query: 301 MMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMA 360
           MMGPHWNPRAWEIGIVTSDEPGVV VGCSSTSANSSKCKNWSFVVEKGD+FVVPRFHPMA
Sbjct: 301 MMGPHWNPRAWEIGIVTSDEPGVVHVGCSSTSANSSKCKNWSFVVEKGDIFVVPRFHPMA 360

Query: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARV 420
           QMSFNNGTFVFVGFSTTNGHNMPQFF GSSSVL++VDREVLAWSFDVNVTT+DRLLKARV
Sbjct: 361 QMSFNNGTFVFVGFSTTNGHNMPQFFVGSSSVLQLVDREVLAWSFDVNVTTVDRLLKARV 420

Query: 421 ESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEEEERKREEEERRKREEEEERKR- 480
           ESI+LECTSCAEEEVRKMEEEAEREREEEEERKREEEE         RRKREEEE+RKR 
Sbjct: 421 ESIILECTSCAEEEVRKMEEEAEREREEEEERKREEEE---------RRKREEEEQRKRX 480

Query: 481 --------EEEEERKREEEEEEERKREEEEERKREEEEEEERKREEEEERKREEEERRK- 540
                   EEEE RKREEEEE ER    EEERKREEEE    +REEE  R+REEEE+R+ 
Sbjct: 481 XXXXXXKREEEERRKREEEEEAER----EEERKREEEE---AQREEERRRRREEEEKREK 540

Query: 541 -REEEEEREREEEEAQKEEEW-RREEEERRKREEEERKREEEETEREKEREE-------- 600
            REEEE+R REEEE Q+EEE  +REEE RR+REEEERKREEEE E+E+E E+        
Sbjct: 541 EREEEEQRRREEEEQQQEEEEAQREEERRRRREEEERKREEEEGEKEREEEQQQEEEEAK 600

Query: 601 ---------EEEREAEREEEEAREREEEHQRERGKRRREGEERQRRGWEEEEEEEEGGGE 644
                    EEEREA+REEEEAREREEEHQRERG+RRRE EE QRR WEEEE E  G  E
Sbjct: 601 REEEEERKREEEREAKREEEEAREREEEHQRERGRRRREAEEGQRRRWEEEEGE--GEEE 656

BLAST of Chy2G029310 vs. NCBI nr
Match: XP_038882657.1 (vicilin-like seed storage protein At2g18540 [Benincasa hispida])

HSP 1 Score: 819 bits (2116), Expect = 3.40e-292
Identity = 522/646 (80.80%), Postives = 553/646 (85.60%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK TA+S  GS  SPS LI I FLFLSLP  ADDGWWE DSPVVKRANERIP+LKTEYG
Sbjct: 1   MKKCTAIS--GSPSSPSFLISILFLFLSLPTNADDGWWETDSPVVKRANERIPLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           EIS VDF DG+RFG YHLQFITLEPNSLFLPVLLH+DMVFY HTGSGRL+WFDD+ L+EV
Sbjct: 61  EISTVDFADGSRFGHYHLQFITLEPNSLFLPVLLHADMVFYTHTGSGRLSWFDDDDLREV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           D+RRGD+YRLHPGSIFYLQS+ ETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF
Sbjct: 121 DIRRGDIYRLHPGSIFYLQSNLETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAK--SSSPWEFEARLLKTFLG 240
            KEVLRKAFMAP+EVIEEIMN KRPPLIVHAA  TPS K K  ++  WE EARLLKTF+G
Sbjct: 181 DKEVLRKAFMAPEEVIEEIMNAKRPPLIVHAAT-TPSKKKKKVAAVAWELEARLLKTFIG 240

Query: 241 GDASATEFNKKKKKKGIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAG 300
           G AS  EFNKKKKK G+YNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLT+G
Sbjct: 241 G-ASGMEFNKKKKK-GVYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTSG 300

Query: 301 SMMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPM 360
           SMMGPHWNP AWEIGIVTSDEPGVVRVGCSST  NSSKCKNWSFVV KGDVFVVPRFHPM
Sbjct: 301 SMMGPHWNPWAWEIGIVTSDEPGVVRVGCSSTK-NSSKCKNWSFVVGKGDVFVVPRFHPM 360

Query: 361 AQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKAR 420
           AQMSFNNGTFVFVGFSTTNGHNMPQF AGSSSVL+IVDREVLAWSFDVNVTT+DRLL AR
Sbjct: 361 AQMSFNNGTFVFVGFSTTNGHNMPQFLAGSSSVLQIVDREVLAWSFDVNVTTVDRLLGAR 420

Query: 421 VESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEEEERKREEEERRKREEEEERKR 480
           VESI+LECTSCAEEEVRKMEEEAEREREEEEE ++ EEEEERKREEEER KREEEEERKR
Sbjct: 421 VESIILECTSCAEEEVRKMEEEAEREREEEEEERKREEEEERKREEEER-KREEEEERKR 480

Query: 481 EEEEERKREEEEEEERKREEEEERKREEEEEEERKREEEEERKREEEERRKREEEEERER 540
           EEEE RKREEEEEE RKREEEEERKREEEEE          RKREEE    RE+EEER R
Sbjct: 481 EEEE-RKREEEEEE-RKREEEEERKREEEEE----------RKREEE----REQEEERRR 540

Query: 541 EEEEAQKEEEWRREEEERRKREEEERKREEEETEREKEREEEEEREAEREEEEAREREEE 600
           EEEEA++EEE RR EEE R+ E+EE + E               REA+REEEEAREREE 
Sbjct: 541 EEEEAKREEERRRREEEERREEKEEERGE---------------REAKREEEEAREREET 600

Query: 601 HQRERGKRRRE----GEERQRRGWEEEEEEEEGGGEEPQLPVLRIL 640
           HQRERG+RRRE     EERQRR WEEE+EEE    EE + P LRIL
Sbjct: 601 HQRERGRRRRETEREAEERQRRRWEEEKEEE----EEEEQPRLRIL 604

BLAST of Chy2G029310 vs. NCBI nr
Match: KAG6603989.1 (Vicilin-like seed storage protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 732 bits (1889), Expect = 1.55e-257
Identity = 482/659 (73.14%), Postives = 527/659 (79.97%), Query Frame = 0

Query: 1   MKKRTAVSVSGSSFSPSILIPIFFLFLSLPAYADDGWWEGDSPVVKRANERIPILKTEYG 60
           MKK TA+S  GS FS S L  +FFLF+SLP+ ADD WWEG  PVVKRANER  +LKTEYG
Sbjct: 1   MKKSTAIS--GSPFSLSFLFTVFFLFVSLPSNADDKWWEGACPVVKRANERRSLLKTEYG 60

Query: 61  EISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWFDDNGLKEV 120
           EISAVD  D ++FG YHLQFIT+EPNSLFLPVLLH+DMV Y+HTGSGRLNWFDD+ L+EV
Sbjct: 61  EISAVDLHDASQFGPYHLQFITMEPNSLFLPVLLHADMVLYMHTGSGRLNWFDDDDLREV 120

Query: 121 DLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAYSRVTDLVRGF 180
           DLRRGD++RL PG+IFY+ SS ETEREKLR+YALFSSTDED F P+IGAYSRVTD VRGF
Sbjct: 121 DLRRGDIFRLQPGAIFYIHSSLETEREKLRMYALFSSTDEDPFEPAIGAYSRVTDHVRGF 180

Query: 181 GKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAKSSSPW--EFEARLLKTFLG 240
            KEVL KAFM P+EVIEEIM+ KRPPLIVHAA  T S K  SS     E EAR LK+F+G
Sbjct: 181 DKEVLCKAFMVPEEVIEEIMDAKRPPLIVHAAT-TLSKKPTSSLSMSLELEARFLKSFIG 240

Query: 241 GDASATEFNKKKKKK-GIYNVYEVDPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTA 300
           G  S  +FNKKKKKK G+YNV+E DPDFENCNGWSLTVTKK SHQLKGSNIGF VVNLTA
Sbjct: 241 GRGSGMDFNKKKKKKKGLYNVFEADPDFENCNGWSLTVTKKVSHQLKGSNIGFFVVNLTA 300

Query: 301 GSMMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHP 360
           GSMMGPHWNPRAWEIGIVTS+E GVVRVGCSS + NSS CK WSFVV KGDVFVVPRFHP
Sbjct: 301 GSMMGPHWNPRAWEIGIVTSEEAGVVRVGCSSMT-NSSICKKWSFVVGKGDVFVVPRFHP 360

Query: 361 MAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKA 420
           MAQMSFNNG+FVFVGFSTTN +N+PQF AG SSVL+ VDREVLAWSFDVNVTTIDRLL A
Sbjct: 361 MAQMSFNNGSFVFVGFSTTNRNNLPQFLAGRSSVLQTVDREVLAWSFDVNVTTIDRLLGA 420

Query: 421 RVESIVLECTSCAEEEVRKMEEEAEREREEEEERKREEEEEERKREEEE--RRKREEEEE 480
           RVES++LECTSCAEEEVRKM EEAERER+EEEERKREEEEEERKREEEE  R+++EEEEE
Sbjct: 421 RVESVILECTSCAEEEVRKMVEEAERERQEEEERKREEEEEERKREEEEEERKRKEEEEE 480

Query: 481 RKREEEEERKREEEEEEERKREEEEERKREEEEEEERKREEEEERKREEEERRKREEEEE 540
           RKREEEE ++                       EEER+R EEEER+REEEE RKREEEE 
Sbjct: 481 RKREEEEAKR-----------------------EEERRRREEEEREREEEEARKREEEE- 540

Query: 541 REREEEEAQK--EEEWRREEEERRKREEEERKREEE--------ETEREKEREEEEEREA 600
           REREEEEA+K  EEE  REEEE RKREEEER+REEE        E  R KE  E  EREA
Sbjct: 541 REREEEEARKREEEEREREEEEARKREEEEREREEEAERERREGEEARRKEEAERGEREA 600

Query: 601 EREEEEAREREEEHQRERGKRRREGEERQRRGWEEEEEEEEGGGEEPQLPVLRILEQWT 644
           ERE EEARE EE H+RERG+RRRE EERQRR  EEEEE           P LRIL Q T
Sbjct: 601 EREAEEARESEEAHRRERGRRRREAEERQRRRREEEEE-----------PTLRILRQRT 620

BLAST of Chy2G029310 vs. TAIR 10
Match: AT2G18540.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 346.7 bits (888), Expect = 4.1e-95
Identity = 309/619 (49.92%), Postives = 410/619 (66.24%), Query Frame = 0

Query: 42  SPVVKRANERIPILKTEYGEISAVDFDDGTRFGRYHLQFITLEPNSLFLPVLLHSDMVFY 101
           SP++ + ++R  ++ TE+G ISAV   DG     YH+QFITLEPN+L LP+LLHSDMVF+
Sbjct: 41  SPLLVKKDQRTSVVATEFGNISAVQIGDG-----YHIQFITLEPNALLLPLLLHSDMVFF 100

Query: 102 VHTGSGRLNWFDDNGLKEVDLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDED 161
           VHTG+G LNW D+   ++++LRRGD++RL  G++FY+ S+     EKLR+YA+F +  + 
Sbjct: 101 VHTGTGILNWIDEESERKLELRRGDVFRLRSGTVFYVHSN-----EKLRVYAIF-NVGKC 160

Query: 162 SFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSIKAK 221
             +P +GAYS V DL+ GF    LR AF  P++++ +I +  +PPLIV+A    P  + +
Sbjct: 161 LNDPCLGAYSSVRDLLLGFDDRTLRSAFAVPEDILRKIRDATKPPLIVNA---LPRNRTQ 220

Query: 222 SSSPWEFEARLLKTFLGGD------ASATEFNKKKKKKGIYNVYEVDPDFENCNGWSLTV 281
                ++++RL++ F+  +      A     +  KKK   +NV+E DPDFEN NG S+ V
Sbjct: 221 GLEEDKWQSRLVRLFVSVEDVTDHLAMKPIVDTNKKKSRTFNVFEEDPDFENNNGRSIVV 280

Query: 282 TKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVVRVGCSSTSANSS 341
            +K+   LKGS  G  +VNLT GSM+GPHWNP A EI IV   E  V  V   S S+  +
Sbjct: 281 DEKDLDALKGSRFGVFMVNLTKGSMIGPHWNPSACEISIVLEGEGMVRVVNQQSLSSCKN 340

Query: 342 KCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGSSSVLKIV 401
             K+ SF+VE+GDVFVVP+FHPMAQMSF N +FVF+GFST+   N PQF  G SSVLK++
Sbjct: 341 DRKSESFMVEEGDVFVVPKFHPMAQMSFENSSFVFMGFSTSAKTNHPQFLVGQSSVLKVL 400

Query: 402 DREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKME---EEAEREREEEEERK 461
           DR+V+A SF+++  TI  LLKA+ ES++ EC SCAE E+ K+    EE +R  EEE ER+
Sbjct: 401 DRDVVAVSFNLSNETIKGLLKAQKESVIFECASCAEGELSKLMREIEERKRREEEEIERR 460

Query: 462 REEEEEERKREEEERR-----KREEEEE---RKREEEEERKREEE---EEEERKREEEEE 521
           R+EEEE RKREE +RR     KR EEEE   +KREEEE RKREEE   EEEE KR EEE 
Sbjct: 461 RKEEEEARKREEAKRREEEEAKRREEEETERKKREEEEARKREEERKREEEEAKRREEER 520

Query: 522 RKREEEEEEERKREEEEE--------------RKREEEERRKREEEEEREREEEEAQKEE 581
           +KREEE E+ RKREEE E              RK  EE  RKR EE+ER+R EEEA+K E
Sbjct: 521 KKREEEAEQARKREEEREKEEEMAKKREEERQRKEREEVERKRREEQERKRREEEARKRE 580

Query: 582 EWRREEEERRKREEEERKREEEETEREKEREEEEEREAEREEEEAREREEEHQRERGKRR 627
           E R+ EEE  KR E+ER+R+E E   E ER+  EE+E +REEE A+ RE+E Q    K+ 
Sbjct: 581 EERKREEEMAKRREQERQRKERE---EVERKIREEQERKREEEMAKRREQERQ----KKE 638

BLAST of Chy2G029310 vs. TAIR 10
Match: AT4G36700.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 1.4e-90
Identity = 238/491 (48.47%), Postives = 329/491 (67.01%), Query Frame = 0

Query: 42  SPVVKRANERIPILKTEYGEISAVDFDDGT-RFGRYHLQFITLEPNSLFLPVLLHSDMVF 101
           SP++ + ++  PI +T++G+IS V   +G    G Y +  ITLEPN++ LP+LLHSDMVF
Sbjct: 45  SPLLIKKDQWKPIFETKFGQISTVQIGNGCGGMGPYKIHSITLEPNTILLPLLLHSDMVF 104

Query: 102 YVHTGSGRLNWFDDNGLKEVDLRRGDLYRLHPGSIFYLQSSFET--EREKLRIYALFSST 161
           +V +GSG LNW D+   K  ++R GD+YRL PGS+FYLQS         KL++YA+FS+ 
Sbjct: 105 FVDSGSGILNWVDEEA-KSTEIRLGDVYRLRPGSVFYLQSKPVDIFLGTKLKLYAIFSNN 164

Query: 162 DEDSFNPSIGAYSRVTDLVRGFGKEVLRKAFMAPDEVIEEIMNTKRPPLIVHAAAPTPSI 221
           DE   +P  GAYS +TDL+ GF + +L+ AF  P+ +IE + N  +PPLIV     TP +
Sbjct: 165 DECLHDPCFGAYSSITDLMFGFDETILQSAFGVPEGIIELMRNRTKPPLIVSETLCTPGV 224

Query: 222 KAKSSSPWEFEARLLKTFLGGDASATEFNKKKKKK---------GIYNVYEVDPDFENCN 281
               ++ W+ + RLLK F  G A   +  KKK+KK           +NV+E +PDFE+  
Sbjct: 225 ----ANTWQLQPRLLKLF-AGSADLVDNKKKKEKKEKKEKVKKAKTFNVFESEPDFESPY 284

Query: 282 GWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIVTSDEPGVVRVGCSS 341
           G ++T+ +K+   LKGS +G  +VNLT GSMMGPHWNP A EI IV     G+VRV  SS
Sbjct: 285 GRTITINRKDLKVLKGSMVGVSMVNLTQGSMMGPHWNPWACEISIVLKG-AGMVRVLRSS 344

Query: 342 TSAN-SSKCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFSTTNGHNMPQFFAGS 401
            S+N SS+CKN  F VE+GD+F VPR HPMAQMSFNN + VFVGF+T+  +N PQF AG 
Sbjct: 345 ISSNTSSECKNVRFKVEEGDIFAVPRLHPMAQMSFNNDSLVFVGFTTSAKNNEPQFLAGE 404

Query: 402 SSVLKIVDREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVRKMEEEAEREREEE 461
            S L+++DR+VLA S +V+  TID LL A+ E+++LEC SCAE E+ K++ E ER++ ++
Sbjct: 405 DSALRMLDRQVLAASLNVSSVTIDGLLGAQKEAVILECHSCAEGEIEKLKVEIERKKIDD 464

Query: 462 EERKREEEEEERKREEEERRKREEEEERKREEEEERKREEEEEEERKREEEEERKREEEE 520
           E ++R    +ERK+EEEE  KREEEE RKREEEEE+KR   ++     +EEE R+R+   
Sbjct: 465 ERKRR---HDERKKEEEE-AKREEEERRKREEEEEKKRWPPQQPP---QEEELRERQLPM 521

BLAST of Chy2G029310 vs. TAIR 10
Match: AT2G28490.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 161.8 bits (408), Expect = 1.9e-39
Identity = 118/422 (27.96%), Postives = 200/422 (47.39%), Query Frame = 0

Query: 54  ILKTEYGEISAVDFDDGTRFGR-YHLQFITLEPNSLFLPVLLHSDMVFYVHTGSGRLNWF 113
           ++K+E GE+  V    G    +  H+ F+T+EP +LF+P  L S ++ ++  G   L   
Sbjct: 94  VIKSEGGEMRVVLSPRGRIIEKPMHIGFLTMEPKTLFVPQYLDSSLLIFIRQGEATLGVI 153

Query: 114 DDNGLKEVDLRRGDLYRLHPGSIFYLQSSFETEREKLRIYALFSSTDEDSFNPSIGAY-- 173
             +   E  L+ GD+Y +  GS+FYL ++   +R  L +      T    F      Y  
Sbjct: 154 CKDEFGERKLKAGDIYWIPAGSVFYLHNTGLGQR--LHVICSIDPTQSLGFETFQPFYIG 213

Query: 174 SRVTDLVRGFGKEVLRKAF-MAPDEVIEEIMNTKRPPLIVHAAAPTPS------------ 233
              + ++ GF    L  AF ++  E+ + +M+  R P++     P P             
Sbjct: 214 GGPSSVLAGFDPHTLTSAFNVSLPELQQMMMSQFRGPIVYVTEGPQPQPQSTVWTQFLGL 273

Query: 234 ------------IKAKSSSP--------WEFEARLLKTFLGGDASATEFNKKKKKKGIYN 293
                       ++ K  SP        W +   ++++ L       + +   + +  YN
Sbjct: 274 RGEEKHKQLKKLLETKQGSPQDQQYSSGWSWR-NIVRSILDLTEEKNKGSGSSECEDSYN 333

Query: 294 VYEV--DPDFENCNGWSLTVTKKNSHQLKGSNIGFLVVNLTAGSMMGPHWNPRAWEIGIV 353
           +Y+    P F+N  GWS+ +   +   LK S IG  +VNLTAG+MM PH NP A E GIV
Sbjct: 334 IYDKKDKPSFDNKYGWSIALDYDDYKPLKHSGIGVYLVNLTAGAMMAPHMNPTATEYGIV 393

Query: 354 TSDEPGVVRVGCSSTSANSSKCKNWSFVVEKGDVFVVPRFHPMAQMSFNNGTFVFVGFST 413
            +    +  V  + TSA +++       V  GDVF +PR+    Q++   G F FVGF+T
Sbjct: 394 LAGSGEIQVVFPNGTSAMNTR-------VSVGDVFWIPRYFAFCQIASRTGPFEFVGFTT 453

Query: 414 TNGHNMPQFFAGSSSVLKIVDREVLAWSFDVNVTTIDRLLKARVESIVLECTSCAEEEVR 438
           +   N PQF  GS+S+L+ ++   L+ +F V+  T+ R ++A+ E+++L   + A   V 
Sbjct: 454 SAHKNRPQFLVGSNSLLRTLNLTSLSIAFGVDEETMRRFIEAQREAVILPTPAAAPPHVG 505

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
F4IQK55.8e-9449.92Vicilin-like seed storage protein At2g18540 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
F4JQG61.9e-8948.47Vicilin-like seed storage protein At4g36700 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q9SK092.6e-3827.96Vicilin-like seed storage protein At2g28490 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Match NameE-valueIdentityDescription
A0A5D3CNA84.0e-26384.27Vicilin-like seed storage protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A0A0KGF21.3e-25886.62PreproMP73 OS=Cucumis sativus OX=3659 GN=Csa_6G502040 PE=4 SV=1[more]
A0A1S3B2F93.8e-25883.74vicilin-like seed storage protein At2g18540 OS=Cucumis melo OX=3656 GN=LOC103485... [more]
A0A6J1GFV72.9e-20572.35vicilin-like seed storage protein At2g18540 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1IU231.6e-20372.88vicilin-like seed storage protein At2g18540 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
XP_011658490.20.091.86vicilin-like seed storage protein At2g18540 [Cucumis sativus] >KAE8647587.1 hypo... [more]
TYK12638.10.084.34vicilin-like seed storage protein [Cucumis melo var. makuwa][more]
XP_008440688.10.083.09PREDICTED: vicilin-like seed storage protein At2g18540 [Cucumis melo][more]
XP_038882657.13.40e-29280.80vicilin-like seed storage protein At2g18540 [Benincasa hispida][more]
KAG6603989.11.55e-25773.14Vicilin-like seed storage protein, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
AT2G18540.14.1e-9549.92RmlC-like cupins superfamily protein [more]
AT4G36700.11.4e-9048.47RmlC-like cupins superfamily protein [more]
AT2G28490.11.9e-3927.96RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 427..621
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 573..587
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 479..644
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 588..617
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 431..457
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 543..572
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 479..535
NoneNo IPR availablePANTHERPTHR31189OS03G0336100 PROTEIN-RELATEDcoord: 19..474
NoneNo IPR availablePANTHERPTHR31189:SF7PREPROMP73coord: 19..474
NoneNo IPR availableCDDcd02244cupin_7S_vicilin-like_Ncoord: 54..211
e-value: 2.9429E-62
score: 202.739
NoneNo IPR availableCDDcd02245cupin_7S_vicilin-like_Ccoord: 263..421
e-value: 1.67858E-64
score: 208.14
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 46..197
e-value: 3.1E-4
score: 16.8
coord: 256..412
e-value: 1.2E-48
score: 177.6
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 256..410
e-value: 4.1E-30
score: 104.4
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 244..428
e-value: 1.3E-43
score: 150.6
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 39..223
e-value: 7.0E-40
score: 138.4
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 23..418

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G029310.1Chy2G029310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane