Chy2G024680 (gene) Cucumber (hystrix) v1

Overview
NameChy2G024680
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionprotein NRT1/ PTR FAMILY 4.5-like
LocationchrH02: 1364600 .. 1366925 (-)
RNA-Seq ExpressionChy2G024680
SyntenyChy2G024680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATGTTTGGCTGTGTGGGTTAAATCTAACATTTTGGGACTTTTAATTTCGCTGATCCTCGGTTAGTTGAAACGTTTAGCTGTTAATTAGTTGTCTTTTCTATATGCAGGAGAAGCTTGAGATTGTTGAAGGAAAAGTGGACTGGAAGGGAAGATTGGCTTACAAACACAAGCATGGAGGAACTAAATCTTCTTTGCTCATACTAGGTACGCAAAATTATTTGCCCATATTCTAACATATATGACAATAACATGAGTCATAGGAAATAAAAAGAAAGGTGGCAGAGCAAAAATTTTAGCTTTCTACACAAACATATTACACACACCACTATATTCCATTTAATCAAAACACAGTTGATTAGTAGTTCAAGCAGCACTCTTGATTACCTTTTGGGTTTCTGTTGCAGCTACATTTGGTTTCGAGAGCATGGCGATCTTTGCGCTTGCAGTAAACTTGATTACGTATTTCAATTCTGTGATGCACTTTGAACTAGCCGATGCTGCCAATCAGTTGACCAATTACATGGGTACTGGTTATATTCTCTCTATTTTGATGGCCATTCTCGCAGACACATACTTCGGCAGAGTCAAAACCGTCATAATTTCTGGCTGCCTCGAGTTTCTGGTTCGTCTAAATTCACCGGCTTTAATTTGCCACCCATACCATTAGTACTACTATGCTATCATATTCTAATCTATAACTGATATTACAGGCGCTTATCTTGCTCATGATACAAGCTCACTACCCAAAGCTCAAGCCACGACCTTGCAATATGTTTGATAAACAATCCCATTGTGAGACAGTTGAAGGAAAAAGTGCTGCCGTGCTCTATGTTGCTCTCTACATATTGGCCACTGGTACTGCAGGCATTAAGGCTGCATTACCATCACATGGTGCTGATCAATTCGATGAAAAAGACCCCAAGGAGGCGATGCAAATGTCCAGCTTCTTCAATAAACTCTTGCTAGGACTGTGCCTTGGTGGTGCTGTTAGTTTAACTCTAATTGTTTGGATCCAAGACTACAAAGGTTGGGACTGGGGTTTAGGTGTATCTTCTGCAGCCATGTTCTTTAGTGTTGTCATCTTTGTTGCTGGAATGCCACTGTACCGAATCCATACTGTTTCTGGATCTAGTACAATCCTCCAAATTCTACAGGTACACATTCTAACAAGCAAAATCCAGAGTATTTTGAGGCAAATATATCATTACATACGTCCAAATGAATATTTTTTTCAGGTATATGTTGCAGCCATCCGTAATAGAAAACTTATTCTCCCTGAAGACTCTACGCATCTTTATGAGATAGAAAGGGACAAGGAGAATGCTATGGAAGACGACTTTCTGCCACATAGAAACATTTACAGGTTTCGTACATGAAACCTTCATTAAATTTTCTGCTTTCGCAGTCCAATAATAGTGTTAAAATATGTATTCAGGTTTTTAGACAAGGCGGCAATTCAACAAACACCTTCTCGGCAAGTTGGAAACCCCGAAGCTTCAAGTGCATGGAAACTATGCACAGTCACCCAAGTAGAGAATGCAAAAATAGTTCTCAGCATGGTACCGATTTTCTGTTGCACAATCATAATGACCCTTTGCTTGGCTCAGCTCCAAACCTTCTCCATCCAACAGGGCCTCACCATGGATACCAAACTAACCAACTCTTTCAGCATCCCTCCGGCTTCACTCCCCATCATCCCAATCTCCTTCATCATCCTCATAGTCCCAATCTACGACAAGATTTTCGTCCCCTTTGCTCGAAAGCTCACAGGGATCCCCACAGGAATCACCCACTTGCAACGAGTAGGTGTTGGATTGGTCCTATCCAGCATTTCCATGGCCGTCGCGGCATTAGTGGAAGTGAAGCGCAAAGGCGTAGCGAGAGACCACAATATGCTGGACGCAACCCCTGTTTCGCAGCCATTGCCAATCAGCACATTTTGGCTTTCTTTTCAATTCTTCATATTCGGAATCGCTGATTTGTTTACATATGTAGGGCTTCTGGAGTTCTTCTACTCCGAGGCGCCGAAAGCCTTGAAATCTGTATCTACATGCTTCCTTTGGAGTTCGATGGCATTGGGATACTTTTTGAGCACCATAGTTGTAAAAATTGTGAACAGAGCTACGGAAGGAATTACGAGAAGTGGAGGTTGGTTGGTAGGAAACAACATAAATCGGAACCATTTGAACCTCTTCTACTGGATGCTTTCAATCTTGAGCTTGATCAACTTCTTTATCTATGTATTTGTTGCAAAGAAATACAAGTACAGAAACCAGAAACCCACCGTCTCCAATGCCGTTAATGACAGTCGGACGCCATAA

mRNA sequence

ATGGAGAAGCTTGAGATTGTTGAAGGAAAAGTGGACTGGAAGGGAAGATTGGCTTACAAACACAAGCATGGAGGAACTAAATCTTCTTTGCTCATACTAGCTACATTTGGTTTCGAGAGCATGGCGATCTTTGCGCTTGCAGTAAACTTGATTACGTATTTCAATTCTGTGATGCACTTTGAACTAGCCGATGCTGCCAATCAGTTGACCAATTACATGGGTACTGGTTATATTCTCTCTATTTTGATGGCCATTCTCGCAGACACATACTTCGGCAGAGTCAAAACCGTCATAATTTCTGGCTGCCTCGAGTTTCTGGCGCTTATCTTGCTCATGATACAAGCTCACTACCCAAAGCTCAAGCCACGACCTTGCAATATGTTTGATAAACAATCCCATTGTGAGACAGTTGAAGGAAAAAGTGCTGCCGTGCTCTATGTTGCTCTCTACATATTGGCCACTGGTACTGCAGGCATTAAGGCTGCATTACCATCACATGGTGCTGATCAATTCGATGAAAAAGACCCCAAGGAGGCGATGCAAATGTCCAGCTTCTTCAATAAACTCTTGCTAGGACTGTGCCTTGGTGGTGCTGTTAGTTTAACTCTAATTGTTTGGATCCAAGACTACAAAGGTTGGGACTGGGGTTTAGGTGTATCTTCTGCAGCCATGTTCTTTAGTGTTGTCATCTTTGTTGCTGGAATGCCACTGTACCGAATCCATACTGTTTCTGGATCTAGTACAATCCTCCAAATTCTACAGGTACACATTCTAACAAGCAAAATCCAGAGTATTTTGAGGCAAATATATCATTACATACGTCCAAATGAATATTTTTTTCAGGTATATGTTGCAGCCATCCGTAATAGAAAACTTATTCTCCCTGAAGACTCTACGCATCTTTATGAGATAGAAAGGGACAAGGAGAATGCTATGGAAGACGACTTTCTGCCACATAGAAACATTTACAGGTTTTTAGACAAGGCGGCAATTCAACAAACACCTTCTCGGCAAGTTGGAAACCCCGAAGCTTCAAGTGCATGGAAACTATGCACAGTCACCCAAGTAGAGAATGCAAAAATAGTTCTCAGCATGGTACCGATTTTCTGTTGCACAATCATAATGACCCTTTGCTTGGCTCAGCTCCAAACCTTCTCCATCCAACAGGGCCTCACCATGGATACCAAACTAACCAACTCTTTCAGCATCCCTCCGGCTTCACTCCCCATCATCCCAATCTCCTTCATCATCCTCATAGTCCCAATCTACGACAAGATTTTCGTCCCCTTTGCTCGAAAGCTCACAGGGATCCCCACAGGAATCACCCACTTGCAACGAGTAGGTGTTGGATTGGTCCTATCCAGCATTTCCATGGCCGTCGCGGCATTAGTGGAAGTGAAGCGCAAAGGCGTAGCGAGAGACCACAATATGCTGGACGCAACCCCTGTTTCGCAGCCATTGCCAATCAGCACATTTTGGCTTTCTTTTCAATTCTTCATATTCGGAATCGCTGATTTGTTTACATATGTAGGGCTTCTGGAGTTCTTCTACTCCGAGGCGCCGAAAGCCTTGAAATCTGTATCTACATGCTTCCTTTGGAGTTCGATGGCATTGGGATACTTTTTGAGCACCATAGTTGTAAAAATTGTGAACAGAGCTACGGAAGGAATTACGAGAAGTGGAGGTTGGTTGGTAGGAAACAACATAAATCGGAACCATTTGAACCTCTTCTACTGGATGCTTTCAATCTTGAGCTTGATCAACTTCTTTATCTATGTATTTGTTGCAAAGAAATACAAGTACAGAAACCAGAAACCCACCGTCTCCAATGCCGTTAATGACAGTCGGACGCCATAA

Coding sequence (CDS)

ATGGAGAAGCTTGAGATTGTTGAAGGAAAAGTGGACTGGAAGGGAAGATTGGCTTACAAACACAAGCATGGAGGAACTAAATCTTCTTTGCTCATACTAGCTACATTTGGTTTCGAGAGCATGGCGATCTTTGCGCTTGCAGTAAACTTGATTACGTATTTCAATTCTGTGATGCACTTTGAACTAGCCGATGCTGCCAATCAGTTGACCAATTACATGGGTACTGGTTATATTCTCTCTATTTTGATGGCCATTCTCGCAGACACATACTTCGGCAGAGTCAAAACCGTCATAATTTCTGGCTGCCTCGAGTTTCTGGCGCTTATCTTGCTCATGATACAAGCTCACTACCCAAAGCTCAAGCCACGACCTTGCAATATGTTTGATAAACAATCCCATTGTGAGACAGTTGAAGGAAAAAGTGCTGCCGTGCTCTATGTTGCTCTCTACATATTGGCCACTGGTACTGCAGGCATTAAGGCTGCATTACCATCACATGGTGCTGATCAATTCGATGAAAAAGACCCCAAGGAGGCGATGCAAATGTCCAGCTTCTTCAATAAACTCTTGCTAGGACTGTGCCTTGGTGGTGCTGTTAGTTTAACTCTAATTGTTTGGATCCAAGACTACAAAGGTTGGGACTGGGGTTTAGGTGTATCTTCTGCAGCCATGTTCTTTAGTGTTGTCATCTTTGTTGCTGGAATGCCACTGTACCGAATCCATACTGTTTCTGGATCTAGTACAATCCTCCAAATTCTACAGGTACACATTCTAACAAGCAAAATCCAGAGTATTTTGAGGCAAATATATCATTACATACGTCCAAATGAATATTTTTTTCAGGTATATGTTGCAGCCATCCGTAATAGAAAACTTATTCTCCCTGAAGACTCTACGCATCTTTATGAGATAGAAAGGGACAAGGAGAATGCTATGGAAGACGACTTTCTGCCACATAGAAACATTTACAGGTTTTTAGACAAGGCGGCAATTCAACAAACACCTTCTCGGCAAGTTGGAAACCCCGAAGCTTCAAGTGCATGGAAACTATGCACAGTCACCCAAGTAGAGAATGCAAAAATAGTTCTCAGCATGGTACCGATTTTCTGTTGCACAATCATAATGACCCTTTGCTTGGCTCAGCTCCAAACCTTCTCCATCCAACAGGGCCTCACCATGGATACCAAACTAACCAACTCTTTCAGCATCCCTCCGGCTTCACTCCCCATCATCCCAATCTCCTTCATCATCCTCATAGTCCCAATCTACGACAAGATTTTCGTCCCCTTTGCTCGAAAGCTCACAGGGATCCCCACAGGAATCACCCACTTGCAACGAGTAGGTGTTGGATTGGTCCTATCCAGCATTTCCATGGCCGTCGCGGCATTAGTGGAAGTGAAGCGCAAAGGCGTAGCGAGAGACCACAATATGCTGGACGCAACCCCTGTTTCGCAGCCATTGCCAATCAGCACATTTTGGCTTTCTTTTCAATTCTTCATATTCGGAATCGCTGATTTGTTTACATATGTAGGGCTTCTGGAGTTCTTCTACTCCGAGGCGCCGAAAGCCTTGAAATCTGTATCTACATGCTTCCTTTGGAGTTCGATGGCATTGGGATACTTTTTGAGCACCATAGTTGTAAAAATTGTGAACAGAGCTACGGAAGGAATTACGAGAAGTGGAGGTTGGTTGGTAGGAAACAACATAAATCGGAACCATTTGAACCTCTTCTACTGGATGCTTTCAATCTTGAGCTTGATCAACTTCTTTATCTATGTATTTGTTGCAAAGAAATACAAGTACAGAAACCAGAAACCCACCGTCTCCAATGCCGTTAATGACAGTCGGACGCCATAA

Protein sequence

MEKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLKPRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIHTVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHLYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKYRNQKPTVSNAVNDSRTP*
Homology
BLAST of Chy2G024680 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 5.5e-126
Identity = 259/610 (42.46%), Postives = 377/610 (61.80%), Query Frame = 0

Query: 2   EKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFE 61
           E++   EG  DW+ R A K +HGG  ++  +L     E++A  A A NL+ Y    MH  
Sbjct: 5   EEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMS 64

Query: 62  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLK 121
            + +AN +TN+MGT ++L++L   L+D +F   +  +IS  +EFL LI+L IQA  P L 
Sbjct: 65  PSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLM 124

Query: 122 PRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQ 181
           P  C+       CE V G  AA+L+V LY++A G  GIK +L SHGA+QFDE  PK   Q
Sbjct: 125 PPSCD----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQ 184

Query: 182 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIH 241
            S+FFN  +  L  G  V++T +VW++D KGW+WG GVS+ A+F S++IF++G   YR  
Sbjct: 185 RSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNK 244

Query: 242 TVSGS--STILQILQVHIL--TSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPED 301
              GS  +TIL++L    +   S   S        + P+ +         + +K +    
Sbjct: 245 IPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNH------CVSKGKKEV---- 304

Query: 302 STHLYEIERDKENAMEDDFLPHR----NIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTV 361
                E + + E   +++ LP R    N  + L+ AA ++   R +           CTV
Sbjct: 305 -----ESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLE----------CTV 364

Query: 362 TQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPI 421
            QVE+ KIVL M+PIF CTI++  CLAQL TFS+QQ  +M+TK+  S  IPPASLPI P+
Sbjct: 365 QQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPV 424

Query: 422 SFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVAR 481
            FI+++ PIYD + +PFARK T   TG+THLQR+GVGLVLS ++MAVAALVE+KRKGVA+
Sbjct: 425 VFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAK 484

Query: 482 DHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLW 541
           D  +LD+    + LP++  W++ Q+   G ADLFT  GLLE+F++EAP +++S++T   W
Sbjct: 485 DSGLLDS---KETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSW 544

Query: 542 SSMALGYFLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYV 601
           +S+A+GY+LS+++V IVN  T G + +  WL G +INR  L+ FYW++ +LS  NF  Y+
Sbjct: 545 ASLAMGYYLSSVIVSIVNSIT-GSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYL 580

Query: 602 FVAKKYKYRN 604
           F A +YKYR+
Sbjct: 605 FWAMRYKYRS 580

BLAST of Chy2G024680 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 9.7e-123
Identity = 252/596 (42.28%), Postives = 361/596 (60.57%), Query Frame = 0

Query: 8   EGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFELADAAN 67
           EG  DW+ + A + +HGG  ++  +LA    E++A  A A NL+ Y  + MH  LA +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 68  QLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLKPRPCNM 127
           ++T +M T ++L++L   LAD +F      +IS  +EFL LILL IQA  P L P PC  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS 132

Query: 128 FDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFN 187
                 CE V G  AA L+V LY+++ G  GIK +LPSHGA+QFDE  PK   Q S+FFN
Sbjct: 133 -SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 188 KLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIHTVSGS- 247
             +  L  G  V++T +VWI+D KGW+WG GVS+ ++F S+++F+ G   Y+     GS 
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 252

Query: 248 -STILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHLYEIE 307
            +TI ++L    L + I S   +                             S H    E
Sbjct: 253 LTTIFKVL----LAASIVSCSSK---------------------------TSSNHFTSRE 312

Query: 308 RDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKIVLSM 367
              E+  +       N    L+KA   +T             W  CTV QVE+ KIVL M
Sbjct: 313 VQSEHEEKTPSQSLTNSLTCLNKAIEGKT----------HHIWLECTVQQVEDVKIVLKM 372

Query: 368 VPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVPIYDK 427
           +PIF CTI++  CLAQL T+S+ Q  TM+ K+ N F++P ASLP+ P+ F++++ P YD 
Sbjct: 373 LPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVN-FNVPSASLPVFPVVFMLILAPTYDH 432

Query: 428 IFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQ 487
           + +PFARK+T    GITHLQR+GVGLVLS ++MAVAALVE+KRK VAR+  +LD+    +
Sbjct: 433 LIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDS---EE 492

Query: 488 PLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI 547
            LPI+  W++ Q+   G ADLFT  GLLEFF++EAP +++S++T   W+S+ALGY+LS++
Sbjct: 493 TLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSV 552

Query: 548 VVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKY 602
           +V IVNR T+   +S  WL G  +NRN L+LFYW++ +LS++NF  Y+F AK+YKY
Sbjct: 553 MVPIVNRVTKSAGQS-PWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Chy2G024680 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 429.9 bits (1104), Expect = 5.0e-119
Identity = 250/609 (41.05%), Postives = 366/609 (60.10%), Query Frame = 0

Query: 8   EGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFELADAAN 67
           E  VDW+GR +  +KHGG +++L +L    FE M I A+  NLITY  + MHF L+ AAN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 68  QLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLKPRPCNM 127
            +TN++GT +I ++L   L+D + G   T+II G +E    ILL +QAH P+LKP  CN 
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 128 FDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFN 187
              Q+ CE  +G  A + ++ALY++A G+  +K  + +HGADQF +  PK++ ++SS+FN
Sbjct: 147 LIDQT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFN 206

Query: 188 KLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIHTVSGSS 247
                  +G  ++LTL+VW+Q + G D G GVS+AAM   ++  V+G   +R        
Sbjct: 207 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPR--- 266

Query: 248 TILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHLYEIERD 307
                          +SI   I H          V VAAI  RKL  P D   L+     
Sbjct: 267 ---------------RSIFTPIAH----------VIVAAILKRKLASPSDPRMLHGDHHV 326

Query: 308 KENAMEDDFLPHRNIYRFLDKAA--IQQTPSRQVGNPEASSAWKLCTVTQVENAKIVLSM 367
             + +    LPH   +RFLDKA   IQ T +++       S W+LCTVTQVE  K ++S+
Sbjct: 327 ANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKE-------SPWRLCTVTQVEQVKTLISL 386

Query: 368 VPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVPIYDK 427
           VPIF  TI+    LAQLQTFS+QQG +M+T+L+NSF IPPASL  IP   +I +VP+YD 
Sbjct: 387 VPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDS 446

Query: 428 IFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQ 487
             VPFARKLTG  +GI  L R+G+GL LS+ SM  AA++E KR    RD ++LD      
Sbjct: 447 FLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKR----RDSSVLDGR---- 506

Query: 488 PLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI 547
              +S FW++ QF IFGI+++FT VGL+EFFY ++ K ++S      + S + G++ S++
Sbjct: 507 --ILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSV 566

Query: 548 VVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKYRNQK 607
           +V +VN+ T     S GWL  N++N++ L+LFYW+L++LSL+NF  Y+F + ++  ++ +
Sbjct: 567 LVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWS-RWNIKSSR 588

Query: 608 PTVSNAVND 615
              +N V D
Sbjct: 627 RNNTNVVGD 588

BLAST of Chy2G024680 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 2.3e-116
Identity = 247/591 (41.79%), Postives = 354/591 (59.90%), Query Frame = 0

Query: 11  VDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFELADAANQLT 70
           VD++GR     KHGGT+++L +L    FE MAI A+  NLITY  + MHF L+ +AN +T
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 71  NYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLKPRPCNMFDK 130
           N++GT ++LS+L   L+D+Y G  +T+++ G +E    ILL +QAH P+L+P  CNM   
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 131 QSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFNKLL 190
             HC    G  AA LY AL ++A G+  +K  + SHGA+QF  KD +   ++SSFFN   
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKDLR---KLSSFFNAAY 206

Query: 191 LGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIHTVSGSSTIL 250
               +G  ++LTL+VW+Q + G D G GVS+A M   ++  VAG   YR    SGS    
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS---- 266

Query: 251 QILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHLYEIERDKEN 310
                 I T                     QV+VAAI  RK I P +   +++   D   
Sbjct: 267 ------IFTP------------------IAQVFVAAITKRKQICPSNPNMVHQPSTDLVR 326

Query: 311 AMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKIVLSMVPIFC 370
                 L H N +RFLDKA I +T  + + +P     W+LCT+ QV   KI+LS++PIF 
Sbjct: 327 VKP---LLHSNKFRFLDKACI-KTQGKAMESP-----WRLCTIEQVHQVKILLSVIPIFA 386

Query: 371 CTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVPIYDKIFVPF 430
           CTII    LAQLQTFS+QQG +M+T +T +F IPPASL  IP   +I  VP+Y+  FVP 
Sbjct: 387 CTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPL 446

Query: 431 ARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPIS 490
           ARKLTG  +GI+ LQR+G GL L++ SM  AALVE KR+    + N++          +S
Sbjct: 447 ARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM----------LS 506

Query: 491 TFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIV 550
            FW++ QF IFG++++FT VGL+EFFY ++ ++++S  T   + S + G++LS+++V  V
Sbjct: 507 IFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTV 566

Query: 551 NRAT--EGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKY 600
           NR T   G     GWL  N++N++ L+ FYW+L+ LS INFF Y+F ++ Y
Sbjct: 567 NRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWY 567

BLAST of Chy2G024680 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 386.7 bits (992), Expect = 4.8e-106
Identity = 227/605 (37.52%), Postives = 347/605 (57.36%), Query Frame = 0

Query: 3   KLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFEL 62
           +L    G VDW+ R A + +HGG  ++  +L     E++A  A A NL+ Y ++ M F  
Sbjct: 37  RLSAWNGYVDWRSRPALRGRHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSP 96

Query: 63  ADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLKP 122
           + AAN +T +MGT + L++L   LAD +F      ++S  +EFL L++L +QAH    +P
Sbjct: 97  SGAANAVTAFMGTAFFLALLGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEP 156

Query: 123 RPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQM 182
                             S   L+V LY++A G  GIK +LP HGA+QFDE+      Q 
Sbjct: 157 -----------------WSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQR 216

Query: 183 SSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIHT 242
           S FFN  +  L  G  +++T++VW++D KGW +G GVS+AA+  SV +F+AG  +YR+  
Sbjct: 217 SFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKV 276

Query: 243 VSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHLY 302
            SGS           +T+  + +   +Y   +             R  ++++   + +  
Sbjct: 277 PSGSP----------ITTLFKVLTAALYAKYKKR-----------RTSRIVVTCHTRNDC 336

Query: 303 EIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKIV 362
           +    K+N   DD      +  FL +          V   E+      CT  QV++ KIV
Sbjct: 337 DDSVTKQNCDGDDGF----LGSFLGEV---------VRERESLPRPLRCTEEQVKDVKIV 396

Query: 363 LSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVPI 422
           + ++PIF  TI++  CLAQL TFS+QQ  TM+TKL  SF++PPA+LP+ P+ F++++ P 
Sbjct: 397 IKILPIFMSTIMLNCCLAQLSTFSVQQASTMNTKL-GSFTVPPAALPVFPVVFMMILAPT 456

Query: 423 YDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARD--HNMLDA 482
           Y+ + +P ARK T   TGITHLQR+G GLVLS ++MAVAALVE KRK V      N   +
Sbjct: 457 YNHLLLPLARKSTKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSS 516

Query: 483 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 542
           +  S PLPI+  W++ Q+   G ADLFT  G++EFF++EAP  ++S++T   W+S+A+GY
Sbjct: 517 SYSSSPLPITFLWVAIQYVFLGSADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGY 576

Query: 543 FLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYK 602
           + S+++V  VN  T G+     WL+G N+N+ HL  FYW++ +LS INF  Y+F A +Y 
Sbjct: 577 YFSSVLVSAVNFVT-GLNHHNPWLLGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYV 588

Query: 603 YRNQK 606
           YR+ +
Sbjct: 637 YRSNQ 588

BLAST of Chy2G024680 vs. ExPASy TrEMBL
Match: A0A5D3CMU7 (Protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008480 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 562/618 (90.94%), Postives = 573/618 (92.72%), Query Frame = 0

Query: 1   MEKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHF 60
           MEKLE+VEGKVDWKGRLAYKHKHGGT+SSLLIL  FGFESMA FALAVNLITYFNSVMHF
Sbjct: 1   MEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHF 60

Query: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKL 120
           ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS C EFLALILLMIQAHYP L
Sbjct: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPNL 120

Query: 121 KPRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAM 180
           KP PCNMFDKQSHCETVEGKSAA+LYVALYILA G AGIKAALPSHGADQFDEKDPKEAM
Sbjct: 121 KPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAM 180

Query: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRI 240
           QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAG+PLYR+
Sbjct: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRM 240

Query: 241 HTVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTH 300
           H VSGSSTILQIL                           QVYVAAIRNRKLILPEDST 
Sbjct: 241 HIVSGSSTILQIL---------------------------QVYVAAIRNRKLILPEDSTQ 300

Query: 301 LYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAK 360
           LYEIERDKE A EDDFLPHRNIYRFLDKAAIQ+TPSRQV NPEASSAWKLCTVTQVENAK
Sbjct: 301 LYEIERDKEVAAEDDFLPHRNIYRFLDKAAIQETPSRQVENPEASSAWKLCTVTQVENAK 360

Query: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIV 420
           IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIP+SFIILIV
Sbjct: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIV 420

Query: 421 PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480
           PIYDKIFVPF RKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA
Sbjct: 421 PIYDKIFVPFTRKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480

Query: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540
           TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY
Sbjct: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540

Query: 541 FLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYK 600
           FLSTIVVKIVNRATEGITRSGGWL+GNNINRNHLNLFYWMLSILS+INFFIY+FVAKKYK
Sbjct: 541 FLSTIVVKIVNRATEGITRSGGWLIGNNINRNHLNLFYWMLSILSMINFFIYIFVAKKYK 591

Query: 601 YRNQKPTVSNAVNDSRTP 619
           YRN KP +S AVNDSRTP
Sbjct: 601 YRNHKPGISKAVNDSRTP 591

BLAST of Chy2G024680 vs. ExPASy TrEMBL
Match: A0A1S4DT56 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103484420 PE=3 SV=1)

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 563/618 (91.10%), Postives = 573/618 (92.72%), Query Frame = 0

Query: 1   MEKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHF 60
           MEKLE+VEGKVDWKGRLAYKHKHGGT+SSLLIL  FGFESMA FALAVNLITYFNSVMHF
Sbjct: 1   MEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHF 60

Query: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKL 120
           ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS C EFLALILLMIQAHYP L
Sbjct: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPNL 120

Query: 121 KPRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAM 180
           KP PCNMFDKQSHCETVEGKSAA+LYVALYILA G AGIKAALPSHGADQFDEKDPKEAM
Sbjct: 121 KPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAM 180

Query: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRI 240
           QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAG+PLYR+
Sbjct: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRM 240

Query: 241 HTVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTH 300
           H VSGSSTILQIL                           QVYVAAIRNRKLILPEDST 
Sbjct: 241 HIVSGSSTILQIL---------------------------QVYVAAIRNRKLILPEDSTQ 300

Query: 301 LYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAK 360
           LYEIERDKE A EDDFLPHRNIYRFLDKAAIQ+TPSRQV NPEASSAWKLCTVTQVENAK
Sbjct: 301 LYEIERDKEVAAEDDFLPHRNIYRFLDKAAIQETPSRQVENPEASSAWKLCTVTQVENAK 360

Query: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIV 420
           IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIP+SFIILIV
Sbjct: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIV 420

Query: 421 PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480
           PIYDKI VPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA
Sbjct: 421 PIYDKILVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480

Query: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540
           TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY
Sbjct: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540

Query: 541 FLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYK 600
           FLSTIVVKIVNRATEGITRSGGWL+GNNINRNHLNLFYWMLSILSLINFFIY+FVAKKYK
Sbjct: 541 FLSTIVVKIVNRATEGITRSGGWLIGNNINRNHLNLFYWMLSILSLINFFIYIFVAKKYK 591

Query: 601 YRNQKPTVSNAVNDSRTP 619
           YRN KP +S AVNDSRTP
Sbjct: 601 YRNHKPGISKAVNDSRTP 591

BLAST of Chy2G024680 vs. ExPASy TrEMBL
Match: A0A5A7UA16 (Protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G001990 PE=3 SV=1)

HSP 1 Score: 1083.2 bits (2800), Expect = 0.0e+00
Identity = 562/618 (90.94%), Postives = 573/618 (92.72%), Query Frame = 0

Query: 1   MEKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHF 60
           MEKLE+VEGKVDWKGRLAYKHKHGGT+SSLLIL  FGFESMA FALAVNLITYFNSVMHF
Sbjct: 1   MEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHF 60

Query: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKL 120
           ELADAANQLTNYMGTGYILSILMAIL DTYFGRVKTVIIS C EFLALILLMIQAHYP L
Sbjct: 61  ELADAANQLTNYMGTGYILSILMAILPDTYFGRVKTVIISSCFEFLALILLMIQAHYPNL 120

Query: 121 KPRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAM 180
           KP PCNMFDKQSHCETVEGKSAA+LYVALYILA G AGIKAALPSHGADQFDEKDPKEAM
Sbjct: 121 KPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAM 180

Query: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRI 240
           QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAG+PLYR+
Sbjct: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRM 240

Query: 241 HTVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTH 300
           H VSGSSTILQIL                           QVYVAAIRNRKLILPEDST 
Sbjct: 241 HIVSGSSTILQIL---------------------------QVYVAAIRNRKLILPEDSTQ 300

Query: 301 LYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAK 360
           LYEIERDKE A EDDFLPHRNIYRFLDKAAIQ+TPSRQV NPEASSAWKLCTVTQVENAK
Sbjct: 301 LYEIERDKEVAAEDDFLPHRNIYRFLDKAAIQETPSRQVENPEASSAWKLCTVTQVENAK 360

Query: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIV 420
           IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIP+SFIILIV
Sbjct: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIV 420

Query: 421 PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480
           PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA
Sbjct: 421 PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480

Query: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540
           TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY
Sbjct: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540

Query: 541 FLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYK 600
           FLSTIVVKIVNRATEGITRSGGWL+GNNINRNHLNLFYWMLSILS+INFFIY+FVAKKYK
Sbjct: 541 FLSTIVVKIVNRATEGITRSGGWLIGNNINRNHLNLFYWMLSILSMINFFIYIFVAKKYK 591

Query: 601 YRNQKPTVSNAVNDSRTP 619
           YRN KP +S AVNDSRTP
Sbjct: 601 YRNHKPGISKAVNDSRTP 591

BLAST of Chy2G024680 vs. ExPASy TrEMBL
Match: A0A1S3AZE0 (protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484420 PE=3 SV=1)

HSP 1 Score: 1081.6 bits (2796), Expect = 0.0e+00
Identity = 562/617 (91.09%), Postives = 572/617 (92.71%), Query Frame = 0

Query: 2   EKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFE 61
           EKLE+VEGKVDWKGRLAYKHKHGGT+SSLLIL  FGFESMA FALAVNLITYFNSVMHFE
Sbjct: 3   EKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHFE 62

Query: 62  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLK 121
           LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS C EFLALILLMIQAHYP LK
Sbjct: 63  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPNLK 122

Query: 122 PRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQ 181
           P PCNMFDKQSHCETVEGKSAA+LYVALYILA G AGIKAALPSHGADQFDEKDPKEAMQ
Sbjct: 123 PPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAMQ 182

Query: 182 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIH 241
           MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAG+PLYR+H
Sbjct: 183 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRMH 242

Query: 242 TVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHL 301
            VSGSSTILQIL                           QVYVAAIRNRKLILPEDST L
Sbjct: 243 IVSGSSTILQIL---------------------------QVYVAAIRNRKLILPEDSTQL 302

Query: 302 YEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKI 361
           YEIERDKE A EDDFLPHRNIYRFLDKAAIQ+TPSRQV NPEASSAWKLCTVTQVENAKI
Sbjct: 303 YEIERDKEVAAEDDFLPHRNIYRFLDKAAIQETPSRQVENPEASSAWKLCTVTQVENAKI 362

Query: 362 VLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVP 421
           VLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIP+SFIILIVP
Sbjct: 363 VLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIVP 422

Query: 422 IYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDAT 481
           IYDKI VPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDAT
Sbjct: 423 IYDKILVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDAT 482

Query: 482 PVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYF 541
           PVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYF
Sbjct: 483 PVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYF 542

Query: 542 LSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKY 601
           LSTIVVKIVNRATEGITRSGGWL+GNNINRNHLNLFYWMLSILSLINFFIY+FVAKKYKY
Sbjct: 543 LSTIVVKIVNRATEGITRSGGWLIGNNINRNHLNLFYWMLSILSLINFFIYIFVAKKYKY 592

Query: 602 RNQKPTVSNAVNDSRTP 619
           RN KP +S AVNDSRTP
Sbjct: 603 RNHKPGISKAVNDSRTP 592

BLAST of Chy2G024680 vs. ExPASy TrEMBL
Match: A0A0A0KHY7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520380 PE=3 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 1.0e-300
Identity = 543/578 (93.94%), Postives = 546/578 (94.46%), Query Frame = 0

Query: 41  MAIFALAVNLITYFNSVMHFELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS 100
           MAIFALAVNLITYFNSVMHFELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS
Sbjct: 1   MAIFALAVNLITYFNSVMHFELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS 60

Query: 101 GCLEFLALILLMIQAHYPKLKPRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIK 160
           GCLEFLALILLMIQAHYPKLKP PCNMFDKQSHCETVEGKSA VLY+ALYILATGTAGIK
Sbjct: 61  GCLEFLALILLMIQAHYPKLKPPPCNMFDKQSHCETVEGKSAVVLYIALYILATGTAGIK 120

Query: 161 AALPSHGADQFDEKDPKEAMQMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVS 220
           AALPSHGADQFDEKDPKEAMQMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVS
Sbjct: 121 AALPSHGADQFDEKDPKEAMQMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVS 180

Query: 221 SAAMFFSVVIFVAGMPLYRIHTVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFF 280
           +AAMFFSVVIFVAGMPLYRIH VSGSSTILQIL                           
Sbjct: 181 AAAMFFSVVIFVAGMPLYRIHIVSGSSTILQIL--------------------------- 240

Query: 281 QVYVAAIRNRKLILPEDSTHLYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVG 340
           QVYVAAIRNRKLILPEDSTHLYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVG
Sbjct: 241 QVYVAAIRNRKLILPEDSTHLYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVG 300

Query: 341 NPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNS 400
           NPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNS
Sbjct: 301 NPEASSAWKLCTVTQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNS 360

Query: 401 FSIPPASLPIIPISFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAV 460
           FSIPPASLPIIP+SFIILIVPIYDKIFVPFARK TGIPTGITHLQRVGVGLVLSSISMAV
Sbjct: 361 FSIPPASLPIIPVSFIILIVPIYDKIFVPFARKFTGIPTGITHLQRVGVGLVLSSISMAV 420

Query: 461 AALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEA 520
           AALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEA
Sbjct: 421 AALVEVKRKGVARDHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEA 480

Query: 521 PKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWM 580
           PKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWM
Sbjct: 481 PKALKSVSTCFLWSSMALGYFLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWM 540

Query: 581 LSILSLINFFIYVFVAKKYKYRNQKPTVSNAVNDSRTP 619
           LSILSLINFFIYVFVAKKYKYRNQKP VSNAVNDSRTP
Sbjct: 541 LSILSLINFFIYVFVAKKYKYRNQKPAVSNAVNDSRTP 551

BLAST of Chy2G024680 vs. NCBI nr
Match: TYK13233.1 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1083 bits (2802), Expect = 0.0
Identity = 562/618 (90.94%), Postives = 573/618 (92.72%), Query Frame = 0

Query: 1   MEKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHF 60
           MEKLE+VEGKVDWKGRLAYKHKHGGT+SSLLIL  FGFESMA FALAVNLITYFNSVMHF
Sbjct: 1   MEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHF 60

Query: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKL 120
           ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS C EFLALILLMIQAHYP L
Sbjct: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPNL 120

Query: 121 KPRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAM 180
           KP PCNMFDKQSHCETVEGKSAA+LYVALYILA G AGIKAALPSHGADQFDEKDPKEAM
Sbjct: 121 KPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAM 180

Query: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRI 240
           QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAG+PLYR+
Sbjct: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRM 240

Query: 241 HTVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTH 300
           H VSGSSTILQILQ                           VYVAAIRNRKLILPEDST 
Sbjct: 241 HIVSGSSTILQILQ---------------------------VYVAAIRNRKLILPEDSTQ 300

Query: 301 LYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAK 360
           LYEIERDKE A EDDFLPHRNIYRFLDKAAIQ+TPSRQV NPEASSAWKLCTVTQVENAK
Sbjct: 301 LYEIERDKEVAAEDDFLPHRNIYRFLDKAAIQETPSRQVENPEASSAWKLCTVTQVENAK 360

Query: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIV 420
           IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIP+SFIILIV
Sbjct: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIV 420

Query: 421 PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480
           PIYDKIFVPF RKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA
Sbjct: 421 PIYDKIFVPFTRKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480

Query: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540
           TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY
Sbjct: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540

Query: 541 FLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYK 600
           FLSTIVVKIVNRATEGITRSGGWL+GNNINRNHLNLFYWMLSILS+INFFIY+FVAKKYK
Sbjct: 541 FLSTIVVKIVNRATEGITRSGGWLIGNNINRNHLNLFYWMLSILSMINFFIYIFVAKKYK 591

Query: 601 YRNQKPTVSNAVNDSRTP 618
           YRN KP +S AVNDSRTP
Sbjct: 601 YRNHKPGISKAVNDSRTP 591

BLAST of Chy2G024680 vs. NCBI nr
Match: XP_016899169.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Cucumis melo])

HSP 1 Score: 1083 bits (2802), Expect = 0.0
Identity = 563/618 (91.10%), Postives = 573/618 (92.72%), Query Frame = 0

Query: 1   MEKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHF 60
           MEKLE+VEGKVDWKGRLAYKHKHGGT+SSLLIL  FGFESMA FALAVNLITYFNSVMHF
Sbjct: 1   MEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHF 60

Query: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKL 120
           ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS C EFLALILLMIQAHYP L
Sbjct: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPNL 120

Query: 121 KPRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAM 180
           KP PCNMFDKQSHCETVEGKSAA+LYVALYILA G AGIKAALPSHGADQFDEKDPKEAM
Sbjct: 121 KPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAM 180

Query: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRI 240
           QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAG+PLYR+
Sbjct: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRM 240

Query: 241 HTVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTH 300
           H VSGSSTILQILQ                           VYVAAIRNRKLILPEDST 
Sbjct: 241 HIVSGSSTILQILQ---------------------------VYVAAIRNRKLILPEDSTQ 300

Query: 301 LYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAK 360
           LYEIERDKE A EDDFLPHRNIYRFLDKAAIQ+TPSRQV NPEASSAWKLCTVTQVENAK
Sbjct: 301 LYEIERDKEVAAEDDFLPHRNIYRFLDKAAIQETPSRQVENPEASSAWKLCTVTQVENAK 360

Query: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIV 420
           IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIP+SFIILIV
Sbjct: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIV 420

Query: 421 PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480
           PIYDKI VPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA
Sbjct: 421 PIYDKILVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480

Query: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540
           TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY
Sbjct: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540

Query: 541 FLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYK 600
           FLSTIVVKIVNRATEGITRSGGWL+GNNINRNHLNLFYWMLSILSLINFFIY+FVAKKYK
Sbjct: 541 FLSTIVVKIVNRATEGITRSGGWLIGNNINRNHLNLFYWMLSILSLINFFIYIFVAKKYK 591

Query: 601 YRNQKPTVSNAVNDSRTP 618
           YRN KP +S AVNDSRTP
Sbjct: 601 YRNHKPGISKAVNDSRTP 591

BLAST of Chy2G024680 vs. NCBI nr
Match: KAA0052592.1 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 1083 bits (2801), Expect = 0.0
Identity = 562/618 (90.94%), Postives = 573/618 (92.72%), Query Frame = 0

Query: 1   MEKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHF 60
           MEKLE+VEGKVDWKGRLAYKHKHGGT+SSLLIL  FGFESMA FALAVNLITYFNSVMHF
Sbjct: 1   MEKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHF 60

Query: 61  ELADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKL 120
           ELADAANQLTNYMGTGYILSILMAIL DTYFGRVKTVIIS C EFLALILLMIQAHYP L
Sbjct: 61  ELADAANQLTNYMGTGYILSILMAILPDTYFGRVKTVIISSCFEFLALILLMIQAHYPNL 120

Query: 121 KPRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAM 180
           KP PCNMFDKQSHCETVEGKSAA+LYVALYILA G AGIKAALPSHGADQFDEKDPKEAM
Sbjct: 121 KPPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAM 180

Query: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRI 240
           QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAG+PLYR+
Sbjct: 181 QMSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRM 240

Query: 241 HTVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTH 300
           H VSGSSTILQILQ                           VYVAAIRNRKLILPEDST 
Sbjct: 241 HIVSGSSTILQILQ---------------------------VYVAAIRNRKLILPEDSTQ 300

Query: 301 LYEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAK 360
           LYEIERDKE A EDDFLPHRNIYRFLDKAAIQ+TPSRQV NPEASSAWKLCTVTQVENAK
Sbjct: 301 LYEIERDKEVAAEDDFLPHRNIYRFLDKAAIQETPSRQVENPEASSAWKLCTVTQVENAK 360

Query: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIV 420
           IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIP+SFIILIV
Sbjct: 361 IVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIV 420

Query: 421 PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480
           PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA
Sbjct: 421 PIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDA 480

Query: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540
           TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY
Sbjct: 481 TPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGY 540

Query: 541 FLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYK 600
           FLSTIVVKIVNRATEGITRSGGWL+GNNINRNHLNLFYWMLSILS+INFFIY+FVAKKYK
Sbjct: 541 FLSTIVVKIVNRATEGITRSGGWLIGNNINRNHLNLFYWMLSILSMINFFIYIFVAKKYK 591

Query: 601 YRNQKPTVSNAVNDSRTP 618
           YRN KP +S AVNDSRTP
Sbjct: 601 YRNHKPGISKAVNDSRTP 591

BLAST of Chy2G024680 vs. NCBI nr
Match: XP_008439704.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucumis melo])

HSP 1 Score: 1082 bits (2797), Expect = 0.0
Identity = 562/617 (91.09%), Postives = 572/617 (92.71%), Query Frame = 0

Query: 2   EKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFE 61
           EKLE+VEGKVDWKGRLAYKHKHGGT+SSLLIL  FGFESMA FALAVNLITYFNSVMHFE
Sbjct: 3   EKLEVVEGKVDWKGRLAYKHKHGGTRSSLLILVAFGFESMANFALAVNLITYFNSVMHFE 62

Query: 62  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLK 121
           LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIIS C EFLALILLMIQAHYP LK
Sbjct: 63  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISSCFEFLALILLMIQAHYPNLK 122

Query: 122 PRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQ 181
           P PCNMFDKQSHCETVEGKSAA+LYVALYILA G AGIKAALPSHGADQFDEKDPKEAMQ
Sbjct: 123 PPPCNMFDKQSHCETVEGKSAAMLYVALYILAIGNAGIKAALPSHGADQFDEKDPKEAMQ 182

Query: 182 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIH 241
           MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAG+PLYR+H
Sbjct: 183 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGLPLYRMH 242

Query: 242 TVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHL 301
            VSGSSTILQILQ                           VYVAAIRNRKLILPEDST L
Sbjct: 243 IVSGSSTILQILQ---------------------------VYVAAIRNRKLILPEDSTQL 302

Query: 302 YEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKI 361
           YEIERDKE A EDDFLPHRNIYRFLDKAAIQ+TPSRQV NPEASSAWKLCTVTQVENAKI
Sbjct: 303 YEIERDKEVAAEDDFLPHRNIYRFLDKAAIQETPSRQVENPEASSAWKLCTVTQVENAKI 362

Query: 362 VLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVP 421
           VLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIP+SFIILIVP
Sbjct: 363 VLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPVSFIILIVP 422

Query: 422 IYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDAT 481
           IYDKI VPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDAT
Sbjct: 423 IYDKILVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDAT 482

Query: 482 PVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYF 541
           PVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYF
Sbjct: 483 PVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYF 542

Query: 542 LSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKY 601
           LSTIVVKIVNRATEGITRSGGWL+GNNINRNHLNLFYWMLSILSLINFFIY+FVAKKYKY
Sbjct: 543 LSTIVVKIVNRATEGITRSGGWLIGNNINRNHLNLFYWMLSILSLINFFIYIFVAKKYKY 592

Query: 602 RNQKPTVSNAVNDSRTP 618
           RN KP +S AVNDSRTP
Sbjct: 603 RNHKPGISKAVNDSRTP 592

BLAST of Chy2G024680 vs. NCBI nr
Match: XP_022142057.1 (protein NRT1/ PTR FAMILY 4.6-like [Momordica charantia])

HSP 1 Score: 1001 bits (2589), Expect = 0.0
Identity = 519/618 (83.98%), Postives = 552/618 (89.32%), Query Frame = 0

Query: 2   EKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFE 61
           EK E+VEGKVDWK R A+KHKHGGT+SS LIL  FGFE++A FALAVNLITYFNSVMHFE
Sbjct: 3   EKFEVVEGKVDWKARSAFKHKHGGTRSSFLILVAFGFENLANFALAVNLITYFNSVMHFE 62

Query: 62  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLK 121
           LADAANQLTNY+GTGYI+SILMAILADTY GRVKTVIISGCLEFLALILLMIQAHYPKLK
Sbjct: 63  LADAANQLTNYLGTGYIISILMAILADTYLGRVKTVIISGCLEFLALILLMIQAHYPKLK 122

Query: 122 PRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQ 181
           P PCNMFDKQSHCETVEG +AA+LYVALYILA GTAGIKAALPSH ADQFDEKDPKEAMQ
Sbjct: 123 PPPCNMFDKQSHCETVEGGNAALLYVALYILAIGTAGIKAALPSHRADQFDEKDPKEAMQ 182

Query: 182 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIH 241
           MSSFFN LLLG+CLGGAVSLTLIVWIQDYKGWDWGLGVSSAA+FFSVVIFVAG+PLYR+H
Sbjct: 183 MSSFFNHLLLGVCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAIFFSVVIFVAGIPLYRMH 242

Query: 242 TVSGSSTILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHL 301
            +SGSS I+QILQ                           VY AAIRNR L+LPEDS  L
Sbjct: 243 IISGSSAIVQILQ---------------------------VYGAAIRNRNLVLPEDSADL 302

Query: 302 YEIERDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKI 361
           YE  RDKE A E+DFLPHRNIYRFLDKAAIQQTPS QV  PEAS+ WKLC VTQVENAKI
Sbjct: 303 YETVRDKEAATEEDFLPHRNIYRFLDKAAIQQTPSGQVETPEASNPWKLCRVTQVENAKI 362

Query: 362 VLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVP 421
           +LSMVPIFCCTI+MTLCLAQLQTFSIQQGLTMDTKLTNSF IPPASLPIIP+SFIILIVP
Sbjct: 363 ILSMVPIFCCTIVMTLCLAQLQTFSIQQGLTMDTKLTNSFHIPPASLPIIPVSFIILIVP 422

Query: 422 IYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDAT 481
           IYD+IFVPFARKLTGIPTGITHLQRVGVGLVLSS+SMAVAAL+EVKRKGVARDHNMLDAT
Sbjct: 423 IYDRIFVPFARKLTGIPTGITHLQRVGVGLVLSSMSMAVAALMEVKRKGVARDHNMLDAT 482

Query: 482 PVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYF 541
           PVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYF
Sbjct: 483 PVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYF 542

Query: 542 LSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKY 601
           LSTIVVK+VN  T+GIT SGGWL+GNNINRNHLNLFYW+LSILSLINF IY+FVAK+YKY
Sbjct: 543 LSTIVVKVVNSVTKGITSSGGWLIGNNINRNHLNLFYWLLSILSLINFSIYLFVAKRYKY 591

Query: 602 RNQ-KPTVSNAVNDSRTP 618
           RN  KP +S+A  D+R P
Sbjct: 603 RNHNKPAISDA--DNRKP 591

BLAST of Chy2G024680 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 453.0 bits (1164), Expect = 3.9e-127
Identity = 259/610 (42.46%), Postives = 377/610 (61.80%), Query Frame = 0

Query: 2   EKLEIVEGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFE 61
           E++   EG  DW+ R A K +HGG  ++  +L     E++A  A A NL+ Y    MH  
Sbjct: 5   EEVSRWEGYADWRNRAAVKGRHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMS 64

Query: 62  LADAANQLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLK 121
            + +AN +TN+MGT ++L++L   L+D +F   +  +IS  +EFL LI+L IQA  P L 
Sbjct: 65  PSKSANDVTNFMGTAFLLALLGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLM 124

Query: 122 PRPCNMFDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQ 181
           P  C+       CE V G  AA+L+V LY++A G  GIK +L SHGA+QFDE  PK   Q
Sbjct: 125 PPSCD----SPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQ 184

Query: 182 MSSFFNKLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIH 241
            S+FFN  +  L  G  V++T +VW++D KGW+WG GVS+ A+F S++IF++G   YR  
Sbjct: 185 RSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNK 244

Query: 242 TVSGS--STILQILQVHIL--TSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPED 301
              GS  +TIL++L    +   S   S        + P+ +         + +K +    
Sbjct: 245 IPCGSPLTTILKVLLAASVKCCSSGSSSNAVASMSVSPSNH------CVSKGKKEV---- 304

Query: 302 STHLYEIERDKENAMEDDFLPHR----NIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTV 361
                E + + E   +++ LP R    N  + L+ AA ++   R +           CTV
Sbjct: 305 -----ESQGELEKPRQEEALPPRAQLTNSLKVLNGAADEKPVHRLLE----------CTV 364

Query: 362 TQVENAKIVLSMVPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPI 421
            QVE+ KIVL M+PIF CTI++  CLAQL TFS+QQ  +M+TK+  S  IPPASLPI P+
Sbjct: 365 QQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKI-GSLKIPPASLPIFPV 424

Query: 422 SFIILIVPIYDKIFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVAR 481
            FI+++ PIYD + +PFARK T   TG+THLQR+GVGLVLS ++MAVAALVE+KRKGVA+
Sbjct: 425 VFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAK 484

Query: 482 DHNMLDATPVSQPLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLW 541
           D  +LD+    + LP++  W++ Q+   G ADLFT  GLLE+F++EAP +++S++T   W
Sbjct: 485 DSGLLDS---KETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSW 544

Query: 542 SSMALGYFLSTIVVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYV 601
           +S+A+GY+LS+++V IVN  T G + +  WL G +INR  L+ FYW++ +LS  NF  Y+
Sbjct: 545 ASLAMGYYLSSVIVSIVNSIT-GSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYL 580

Query: 602 FVAKKYKYRN 604
           F A +YKYR+
Sbjct: 605 FWAMRYKYRS 580

BLAST of Chy2G024680 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 442.2 bits (1136), Expect = 6.9e-124
Identity = 252/596 (42.28%), Postives = 361/596 (60.57%), Query Frame = 0

Query: 8   EGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFELADAAN 67
           EG  DW+ + A + +HGG  ++  +LA    E++A  A A NL+ Y  + MH  LA +++
Sbjct: 9   EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 68

Query: 68  QLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLKPRPCNM 127
           ++T +M T ++L++L   LAD +F      +IS  +EFL LILL IQA  P L P PC  
Sbjct: 69  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS 128

Query: 128 FDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFN 187
                 CE V G  AA L+V LY+++ G  GIK +LPSHGA+QFDE  PK   Q S+FFN
Sbjct: 129 -SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 188

Query: 188 KLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIHTVSGS- 247
             +  L  G  V++T +VWI+D KGW+WG GVS+ ++F S+++F+ G   Y+     GS 
Sbjct: 189 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 248

Query: 248 -STILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHLYEIE 307
            +TI ++L    L + I S   +                             S H    E
Sbjct: 249 LTTIFKVL----LAASIVSCSSK---------------------------TSSNHFTSRE 308

Query: 308 RDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKIVLSM 367
              E+  +       N    L+KA   +T             W  CTV QVE+ KIVL M
Sbjct: 309 VQSEHEEKTPSQSLTNSLTCLNKAIEGKT----------HHIWLECTVQQVEDVKIVLKM 368

Query: 368 VPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVPIYDK 427
           +PIF CTI++  CLAQL T+S+ Q  TM+ K+ N F++P ASLP+ P+ F++++ P YD 
Sbjct: 369 LPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVN-FNVPSASLPVFPVVFMLILAPTYDH 428

Query: 428 IFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQ 487
           + +PFARK+T    GITHLQR+GVGLVLS ++MAVAALVE+KRK VAR+  +LD+    +
Sbjct: 429 LIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDS---EE 488

Query: 488 PLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI 547
            LPI+  W++ Q+   G ADLFT  GLLEFF++EAP +++S++T   W+S+ALGY+LS++
Sbjct: 489 TLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSV 548

Query: 548 VVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKY 602
           +V IVNR T+   +S  WL G  +NRN L+LFYW++ +LS++NF  Y+F AK+YKY
Sbjct: 549 MVPIVNRVTKSAGQS-PWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 556

BLAST of Chy2G024680 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 442.2 bits (1136), Expect = 6.9e-124
Identity = 252/596 (42.28%), Postives = 361/596 (60.57%), Query Frame = 0

Query: 8   EGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFELADAAN 67
           EG  DW+ + A + +HGG  ++  +LA    E++A  A A NL+ Y  + MH  LA +++
Sbjct: 13  EGYADWRNKAALRGRHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSS 72

Query: 68  QLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLKPRPCNM 127
           ++T +M T ++L++L   LAD +F      +IS  +EFL LILL IQA  P L P PC  
Sbjct: 73  EVTTFMATAFLLALLGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKS 132

Query: 128 FDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFN 187
                 CE V G  AA L+V LY+++ G  GIK +LPSHGA+QFDE  PK   Q S+FFN
Sbjct: 133 -SAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFN 192

Query: 188 KLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIHTVSGS- 247
             +  L  G  V++T +VWI+D KGW+WG GVS+ ++F S+++F+ G   Y+     GS 
Sbjct: 193 YYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSP 252

Query: 248 -STILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHLYEIE 307
            +TI ++L    L + I S   +                             S H    E
Sbjct: 253 LTTIFKVL----LAASIVSCSSK---------------------------TSSNHFTSRE 312

Query: 308 RDKENAMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKIVLSM 367
              E+  +       N    L+KA   +T             W  CTV QVE+ KIVL M
Sbjct: 313 VQSEHEEKTPSQSLTNSLTCLNKAIEGKT----------HHIWLECTVQQVEDVKIVLKM 372

Query: 368 VPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVPIYDK 427
           +PIF CTI++  CLAQL T+S+ Q  TM+ K+ N F++P ASLP+ P+ F++++ P YD 
Sbjct: 373 LPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVN-FNVPSASLPVFPVVFMLILAPTYDH 432

Query: 428 IFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQ 487
           + +PFARK+T    GITHLQR+GVGLVLS ++MAVAALVE+KRK VAR+  +LD+    +
Sbjct: 433 LIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDS---EE 492

Query: 488 PLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI 547
            LPI+  W++ Q+   G ADLFT  GLLEFF++EAP +++S++T   W+S+ALGY+LS++
Sbjct: 493 TLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSV 552

Query: 548 VVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKY 602
           +V IVNR T+   +S  WL G  +NRN L+LFYW++ +LS++NF  Y+F AK+YKY
Sbjct: 553 MVPIVNRVTKSAGQS-PWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKY 560

BLAST of Chy2G024680 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 429.9 bits (1104), Expect = 3.5e-120
Identity = 250/609 (41.05%), Postives = 366/609 (60.10%), Query Frame = 0

Query: 8   EGKVDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFELADAAN 67
           E  VDW+GR +  +KHGG +++L +L    FE M I A+  NLITY  + MHF L+ AAN
Sbjct: 27  EESVDWRGRPSNPNKHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAAN 86

Query: 68  QLTNYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLKPRPCNM 127
            +TN++GT +I ++L   L+D + G   T+II G +E    ILL +QAH P+LKP  CN 
Sbjct: 87  IVTNFVGTIFIFALLGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNP 146

Query: 128 FDKQSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFN 187
              Q+ CE  +G  A + ++ALY++A G+  +K  + +HGADQF +  PK++ ++SS+FN
Sbjct: 147 LIDQT-CEEAKGFKAMIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFN 206

Query: 188 KLLLGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIHTVSGSS 247
                  +G  ++LTL+VW+Q + G D G GVS+AAM   ++  V+G   +R        
Sbjct: 207 AAYFAFSMGELIALTLLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPR--- 266

Query: 248 TILQILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHLYEIERD 307
                          +SI   I H          V VAAI  RKL  P D   L+     
Sbjct: 267 ---------------RSIFTPIAH----------VIVAAILKRKLASPSDPRMLHGDHHV 326

Query: 308 KENAMEDDFLPHRNIYRFLDKAA--IQQTPSRQVGNPEASSAWKLCTVTQVENAKIVLSM 367
             + +    LPH   +RFLDKA   IQ T +++       S W+LCTVTQVE  K ++S+
Sbjct: 327 ANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKE-------SPWRLCTVTQVEQVKTLISL 386

Query: 368 VPIFCCTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVPIYDK 427
           VPIF  TI+    LAQLQTFS+QQG +M+T+L+NSF IPPASL  IP   +I +VP+YD 
Sbjct: 387 VPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIMLIFLVPLYDS 446

Query: 428 IFVPFARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQ 487
             VPFARKLTG  +GI  L R+G+GL LS+ SM  AA++E KR    RD ++LD      
Sbjct: 447 FLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKR----RDSSVLDGR---- 506

Query: 488 PLPISTFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTI 547
              +S FW++ QF IFGI+++FT VGL+EFFY ++ K ++S      + S + G++ S++
Sbjct: 507 --ILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYFSSV 566

Query: 548 VVKIVNRATEGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKYKYRNQK 607
           +V +VN+ T     S GWL  N++N++ L+LFYW+L++LSL+NF  Y+F + ++  ++ +
Sbjct: 567 LVSVVNKITSTSVDSKGWLGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWS-RWNIKSSR 588

Query: 608 PTVSNAVND 615
              +N V D
Sbjct: 627 RNNTNVVGD 588

BLAST of Chy2G024680 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 421.0 bits (1081), Expect = 1.6e-117
Identity = 247/591 (41.79%), Postives = 354/591 (59.90%), Query Frame = 0

Query: 11  VDWKGRLAYKHKHGGTKSSLLILATFGFESMAIFALAVNLITYFNSVMHFELADAANQLT 70
           VD++GR     KHGGT+++L +L    FE MAI A+  NLITY  + MHF L+ +AN +T
Sbjct: 27  VDFRGRPCRPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVT 86

Query: 71  NYMGTGYILSILMAILADTYFGRVKTVIISGCLEFLALILLMIQAHYPKLKPRPCNMFDK 130
           N++GT ++LS+L   L+D+Y G  +T+++ G +E    ILL +QAH P+L+P  CNM   
Sbjct: 87  NFIGTVFLLSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKST 146

Query: 131 QSHCETVEGKSAAVLYVALYILATGTAGIKAALPSHGADQFDEKDPKEAMQMSSFFNKLL 190
             HC    G  AA LY AL ++A G+  +K  + SHGA+QF  KD +   ++SSFFN   
Sbjct: 147 TIHCVEANGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKDLR---KLSSFFNAAY 206

Query: 191 LGLCLGGAVSLTLIVWIQDYKGWDWGLGVSSAAMFFSVVIFVAGMPLYRIHTVSGSSTIL 250
               +G  ++LTL+VW+Q + G D G GVS+A M   ++  VAG   YR    SGS    
Sbjct: 207 FAFSMGQLIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGS---- 266

Query: 251 QILQVHILTSKIQSILRQIYHYIRPNEYFFQVYVAAIRNRKLILPEDSTHLYEIERDKEN 310
                 I T                     QV+VAAI  RK I P +   +++   D   
Sbjct: 267 ------IFTP------------------IAQVFVAAITKRKQICPSNPNMVHQPSTDLVR 326

Query: 311 AMEDDFLPHRNIYRFLDKAAIQQTPSRQVGNPEASSAWKLCTVTQVENAKIVLSMVPIFC 370
                 L H N +RFLDKA I +T  + + +P     W+LCT+ QV   KI+LS++PIF 
Sbjct: 327 VKP---LLHSNKFRFLDKACI-KTQGKAMESP-----WRLCTIEQVHQVKILLSVIPIFA 386

Query: 371 CTIIMTLCLAQLQTFSIQQGLTMDTKLTNSFSIPPASLPIIPISFIILIVPIYDKIFVPF 430
           CTII    LAQLQTFS+QQG +M+T +T +F IPPASL  IP   +I  VP+Y+  FVP 
Sbjct: 387 CTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIFFVPLYETFFVPL 446

Query: 431 ARKLTGIPTGITHLQRVGVGLVLSSISMAVAALVEVKRKGVARDHNMLDATPVSQPLPIS 490
           ARKLTG  +GI+ LQR+G GL L++ SM  AALVE KR+    + N++          +S
Sbjct: 447 ARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM----------LS 506

Query: 491 TFWLSFQFFIFGIADLFTYVGLLEFFYSEAPKALKSVSTCFLWSSMALGYFLSTIVVKIV 550
            FW++ QF IFG++++FT VGL+EFFY ++ ++++S  T   + S + G++LS+++V  V
Sbjct: 507 IFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCSYSFGFYLSSVLVSTV 566

Query: 551 NRAT--EGITRSGGWLVGNNINRNHLNLFYWMLSILSLINFFIYVFVAKKY 600
           NR T   G     GWL  N++N++ L+ FYW+L+ LS INFF Y+F ++ Y
Sbjct: 567 NRVTSSNGSGTKEGWLGDNDLNKDRLDHFYWLLASLSFINFFNYLFWSRWY 567

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1575.5e-12642.46Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE49.7e-12342.28Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q93VV55.0e-11941.05Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1[more]
Q56XQ62.3e-11641.79Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1[more]
Q9FM204.8e-10637.52Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A5D3CMU70.0e+0090.94Protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S4DT560.0e+0091.10protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A5A7UA160.0e+0090.94Protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3AZE00.0e+0091.09protein NRT1/ PTR FAMILY 4.6-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0KHY71.0e-30093.94Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G520380 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
TYK13233.10.090.94protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Cucumis melo var. makuwa][more]
XP_016899169.10.091.10PREDICTED: protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Cucumis melo][more]
KAA0052592.10.090.94protein NRT1/ PTR FAMILY 4.5-like isoform X2 [Cucumis melo var. makuwa][more]
XP_008439704.10.091.09PREDICTED: protein NRT1/ PTR FAMILY 4.6-like isoform X1 [Cucumis melo][more]
XP_022142057.10.083.98protein NRT1/ PTR FAMILY 4.6-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT1G69850.13.9e-12742.46nitrate transporter 1:2 [more]
AT1G27040.26.9e-12442.28Major facilitator superfamily protein [more]
AT1G27040.16.9e-12442.28Major facilitator superfamily protein [more]
AT1G59740.13.5e-12041.05Major facilitator superfamily protein [more]
AT1G33440.11.6e-11741.79Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 1..607
e-value: 7.2E-162
score: 541.2
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 32..603
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 95..555
e-value: 1.7E-81
score: 274.1
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 10..259
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 280..608
NoneNo IPR availablePANTHERPTHR11654:SF336PROTEIN NRT1/ PTR FAMILY 4.2coord: 280..608
NoneNo IPR availablePANTHERPTHR11654:SF336PROTEIN NRT1/ PTR FAMILY 4.2coord: 10..259

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G024680.1Chy2G024680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042981 regulation of apoptotic process
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity