Chy2G024160 (gene) Cucumber (hystrix) v1

Overview
NameChy2G024160
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionalpha-1,2-Mannosidase
LocationchrH02: 1080625 .. 1086154 (+)
RNA-Seq ExpressionChy2G024160
SyntenyChy2G024160
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGTTCTCTACCTTACTCTGTCAAGGATGTTCACTACGACAATGCTAAGTTTCGACAGAGATCCTACGTCAAGGTTTTTCTATGCTAGATCTTGACTTAATTGCTCCATTTCGATTTCATGAAATCTGATTGCTTATCAGGCACTCCATGTGTGCGATTTTTTTTATTCGATTAGTGTTTGGATGAATTGGTAGCTTTCTAGTTACATTCTAGCAACGATTATTGCTTAATAATTGTTCGTTCCTTGAACTTTGATTGTGCCTGGAAAAACTTTAGATTCTCGAGGTGAAAATTTTATTGCCGATTCCATTTACTTTATTTTAAGACTGCGATGTTCATTCTGGAATGGAGGTGGCTCGGGGGAGGATTAAAAACTCGACGGAAATGATATCCGGATTTGTTTGAATAGTCATTTTCGTCGGAAATGCGTGCATAACTGGTGTAAATTATCATAGAAACGCGAGACTGAAGATACTTGTGTTTTGCGAATTTGCCAGGAGCTATGTTGCCAGTTTAGATCCTATGGTTACTTGTTGGTTTGTTGTGACCGCTGACCAACCTTTAATCTGTAGCGTGATGGATACTAGGTCATCGGTGATTGTTGTGAAAGCACACAACGTCGTTCGTAAAAGTATAATCTGTGATGGGTGGTTATACACGTTGTCTTCTTCTACTGTAAATTTCAGACTTACCAATACTTTTGGATGACTGCCCAGACAATATGAAAGAACCATAGAAGATCAATTTTCAAGGAAGAATAATTCTAGAGAATACTTTTAGTGACAAGAAATTTCTCCCTCCAAATTTCTCCTTACATGTTGAACTGCTCGTCTAGAACGTGGGACATGCAACTTATTTCCAAAATACTCGTCCCATGTTGTGAGATCATTGTGATCGGTAACTCTGGGAATTTGTACGGTTTTAATTATCTTAACACGGAATATAGTGGCCTAATAATTTTGTATTTATTTATTTATTATGTCATATCCTAGTAAAATAAAGATCTTATTTTGGTGGTACAAAAATTAGATTTTATACAAAAGGAAGTGTATTGTTTGAACTAATCCTTATTGGTTACAGACTGCACTTATCAGAATCTTCCATTTATGGGATCAACCCAGAGTTATTTGATAGGGATGTACATTATTTGTTATGAGTTATGACTCAAAGTTCTCATCTCGTTTTCTCAGGTGATGACTCAGAATTTACTCACTGGTAGCAGAAAACGTGATTATCTAAGTTGTAGCACAGGAAAATTTCTTGTATTACTTTTGATCTTCGGTTTGGCATATCTTTTATTGACAAGTGCTACTCCTGTCCATGTTCCCAACCAACTTGCCAAAGTTAGCGAGAATAGTGAGAAACAAGAGATTAAATATAATGGTGGCATCAACTTCAGGAACTTATGGAGGAAAGCTCCTAGGCTTCCCCCTCAATTACCACCAGATGAAAAAGGTAGCAATACTAGCTATCATATCGATCCAAACACGTTAATCTCTGAGTCTTTATGGATTTCAAGGCAACAGAAAGTAAAGGATGCTTTTATCCATGCATGGTCCGGCTATAAAAGGTATGCAATGGGGTATGATGAACTTATGCCACTGAGCCAGCATGGAACCGATGGGTTAGGAGGTTTAGGTGCTACAGTTGTGGATGCCCTTGACACTGCCATGATAATGGGCGCTAGTGATATTGTCTCTGAGGCAGGCTCATGGATTGAAAAAAACCTTATGGATAGAATTAAGGAAAAAGGACAAGTCAATTTATTTGAAACTACCATACGTGTCCTAGGTGGTCTCTTGAGTGCATATCACTTGAGAGCTGGAGAGTATGGGAAGACCTTTGGAAATGAAGGACCCAAACCTGCTGTTTATCTAGAAATTGCGAAGAACTTGGCTGATCGTTTGCTAAGTGCTTTTACCTCCAGTCCAACTGCTATTCCTCTCTGTGATGTTGTTTTAAAAGATTCCTCTGCCCATGCAGCTCCTGGTGGATCAAGTAGTACATCCGAAGTATCCACTTTGCAGCTGGAATTTAATTATCTTAGTACTGTTTCAGGCGACCCAAAGTATTCAATTGCTGCTATGAAGGTCTTGGAACACATGAAGAGCCTTCCGAAAGTGGAAGGTTTGGTTCCCATCTATATTAGGTATGGAGCTTTTATGTCTGGCTATAAGTAATTTACTTTATTTTAGACAGTATGGATACGTCTTAACATTTGTGTCAAACTTCGAATGCCCTTTTGTTATGTGATCTGTGGATGAATCAGAAAAAAAAAAAAATTAAATTATGAATTCTTTTGTAAACTTCTCTAGTTTTCATCTGGGTTGATAGCATATCTCCCGGGACATTTTCTGGTATACCTGACTTTCGGTATTAATGTAGTACAAACTGGTGCGTGAATGTAATCAATTCAATGAATATTTCTAGTATATATTTTATGTCAAACTTTCTATTTTCTTTTATTGATATATATATATTTTTCGGGCTTCTCATTGTGGATAAATGAAATCACTATTATTTGAGACGAAATTTTTTGGGCATCAAAATCATTCTGATGTGTCAAGATAGGCAAACCTAAATAATGTATCATTAGAAATATCATCTTTGTCAATTATAATCTCAGTGTCTGAGATATGACTTCAATTGAGCCTTTTGGCATGTTGGCAAAATTCTCTTTCCAATCAAGAGATGTTATTCTTCTGAAGCCAAGAGAAAGTAAATTGTTACGCTTGTACCAGTTTTATTTTCCTTTTCGGGAACATATGATCCTGTGGCTGTTAATCCTTTCAAGCATTATGTAAGAACCCTAGTTAGGGAAATAATAAGATTATTAGTTGAATATTAGTATGGGTTTTAGAAGGGACAAAATAGTAGTTAGTTAGTAAGTTTGTTCGGGTTTTTGTTATAAATAGAAGGTGTGGGTTGAGAGGAAGTGGGAAAATTTTTGTATGGTGAATTCCTAATTGGGGAATTTTGGGAGAGGATTTCCAGCCCTCTTGAAAGTGCTGGTTTGGGTACCTAACACATTAATCCTGAAAAAGCTCGCTTTGAATTTCATGAGTTTTGGTCTAATGTGGTTCCTTTCACCATTCTAAATCTTCTCGTGCTTTTCAGTTCTTGAAATGTTCCTTTAATTTAAAGGATATGGTGATGACCAAATTTACTTATTTTCGCGAAATTTTCTTCTCGATTGCAGCCCTCATACTGGCGAATTTAGTGGTGAAACCATAAGACTGGGATCTCGTGGTGATAGCTACTACGAGTATCTACTGAAAGTGTGGCTTCAGAAGCGAACAAGCGGAAATAATAATTTCACTTACCTACATGATATGTATGAAGAAGCCATGAAAGGTGTTAAACATCTTCTTGTTCGGAAGTCTATTCCAAATGGGTTGGTTTTTGTTGGAGAATTGCCCTATGGATCGGATTCTACTATTAGTCCGAAGATGGATCATCTGGTATGTATGATTTCCTCCTGTATCAAATATGCACGTTTCTGGTAGACCAGCTTATGGTATAGTCAAGGCTATATATCAAGGAACATTGGATCAAACTGAGAAAAAATTTGGAAGATTAACCCCAATTTGGGAATTGTTTGACTTTTGATTTGTCTAAGGTATTCTACTTCGAAATGTCTCTGGCTGCTTAGAAATATGCTTTAAAATTTGAAAAAGAATGTGACGTATGATAAAGATGGTATATGTATTCGTTGATTGACATAGTATAGGGCTCTATACATTCATTCGCCCTCAAGATCAGTATTAGGGTAACAATGGAAACAGTACATTATACAAACTTCTTTCTCTGCTGCTTTGTTCACAAATGGCCGTTTCATCTGTTTCTATGCAGTGAGGCAAATTTTATGCTTTAGGGCTTCTTTTCGTTTTATCTGTACAGTGTTGCAATTTATTTGTAGTATGAACACGTCTCCAACATAACATTTAATAGCCTACTAGTTATTCAATCGAACTGATGCTACCGGAAAGAAAGTAAATGTCATTGGCAACATGAATATATCTTTCCTGTTCTCTTTAATGAATACAAGGAGATAGGGAGAAAGTCTATTTGGTGATGAGATTAGATGATGCAGGGGAAAAGAGGATATGCAAATACTGGTAAATGGGGGGAATGTATACATGAGGAAAGCCTTTATTTAGTTAGTTTTCAAAATAAATAGTAAATAAAAATCAAAGTGACCGTTAGCTATCATCCTACCCGTTCGTAGGTTAACACTTGGGCAATTCACGATTGTGCTTTGTACTGCACTTTATTGGTGTAAATCTGTTCCCCTTTATAGATTAATACATAATCTCTCACCTTTCGCTAGTTGGAGGTCCTTTGTAATGGTGGATGGGTTCTACTTCTACCCATTGTTGTTGTCCATTTCATTCATCGGTGATACTCCTCAATATATATATATATCTGGACTTGTATCTATTTCGGTATTTAGTTGATGAGTTTACTAAGCCTGCATATTTTCCTTCATTATTCTGATGTGATTTTATTTGAAGACTTAATTTATTCACCAGCTGAATTGTAATGGGTTCTACATCACGATTTCAAATTAATACTTTGATTCATCAGATTATTGGCTATCTGAATAAAGCTATGTGACATAATAAAGCATCATAGCAATCCTTGTTTTGTTTTGGAAAAGTGCTTAAACTAATAAACTTGTGCTTGTTTTAGGTATGTTTCTTATCAGGTACCCTTGCTCTTGGTGCTACCAAGGGCATCACCAAGGAAGAAGCCATGAGTAAAAGCTTGTTGAACTTTGATGATCTGGAAAATCTAAATCTGGCAGAGGATCTTGCAAAAACTTGCTTTGAAATGTATGAAGTAACCTCTACCGGTCTTGCCCCAGAAATTGCTTATTTTCATACTGAGGTCGGATTTATTTCCCATCATCTATTGTAAATTTGTTCGCTATATCTATTTTTCCCCTTTTCTTCATGCTGTCCAATAATCTAAAAACTCCACGCACATTCTTGTCCAGGAATATTCTGAACAAGGCCTTGATGGTGGAAATAAAAATTCGAAATACATCGATGACATAATAATCAAGCCACTGGATCGTCATAATCTTATGCGGCCCGAAACAGTTGAGTCATTGTTTGTCCTGTATCGTATCACAGGCGATCCAAAGTATGGTTTCTATATCATTAACCACGCCTCTTGTATTGTTCATTAACTGTGTTTCGTTTATTCACATTGGATGAGAAAGAAAAAAAAAAAGAAGGATGCTTACATGAACCTATTGCAGGTATCGTGCATGGGGGTGGGAGATTTTCGAAGCATTCGAGAGGCACACAAAGGTTAGTAGTGGTGGATATACATCACTAGATGATGTTACTGCGGTTCCTCCTCGGAGAAGGGACAAAATGGAGACATTCTTCCTGGGGGAGACACTCAAGTATTTGTACTTGCTATTTGGGGACAGTTCCACGATCCCATTGGATAAATATGTGTTCAATACTGAAGCACATCCTCTACCAATGGAAGGCAATATTCAGGTTCAATGA

mRNA sequence

ATGTCGAGTTCTCTACCTTACTCTGTCAAGGATGTTCACTACGACAATGCTAAGTTTCGACAGAGATCCTACGTCAAGGTGATGACTCAGAATTTACTCACTGGTAGCAGAAAACGTGATTATCTAAGTTGTAGCACAGGAAAATTTCTTGTATTACTTTTGATCTTCGGTTTGGCATATCTTTTATTGACAAGTGCTACTCCTGTCCATGTTCCCAACCAACTTGCCAAAGTTAGCGAGAATAGTGAGAAACAAGAGATTAAATATAATGGTGGCATCAACTTCAGGAACTTATGGAGGAAAGCTCCTAGGCTTCCCCCTCAATTACCACCAGATGAAAAAGGTAGCAATACTAGCTATCATATCGATCCAAACACGTTAATCTCTGAGTCTTTATGGATTTCAAGGCAACAGAAAGTAAAGGATGCTTTTATCCATGCATGGTCCGGCTATAAAAGGTATGCAATGGGGTATGATGAACTTATGCCACTGAGCCAGCATGGAACCGATGGGTTAGGAGGTTTAGGTGCTACAGTTGTGGATGCCCTTGACACTGCCATGATAATGGGCGCTAGTGATATTGTCTCTGAGGCAGGCTCATGGATTGAAAAAAACCTTATGGATAGAATTAAGGAAAAAGGACAAGTCAATTTATTTGAAACTACCATACGTGTCCTAGGTGGTCTCTTGAGTGCATATCACTTGAGAGCTGGAGAGTATGGGAAGACCTTTGGAAATGAAGGACCCAAACCTGCTGTTTATCTAGAAATTGCGAAGAACTTGGCTGATCGTTTGCTAAGTGCTTTTACCTCCAGTCCAACTGCTATTCCTCTCTGTGATGTTGTTTTAAAAGATTCCTCTGCCCATGCAGCTCCTGGTGGATCAAGTAGTACATCCGAAGTATCCACTTTGCAGCTGGAATTTAATTATCTTAGTACTGTTTCAGGCGACCCAAAGTATTCAATTGCTGCTATGAAGGTCTTGGAACACATGAAGAGCCTTCCGAAAGTGGAAGGTTTGGTTCCCATCTATATTAGCCCTCATACTGGCGAATTTAGTGGTGAAACCATAAGACTGGGATCTCGTGGTGATAGCTACTACGAGTATCTACTGAAAGTGTGGCTTCAGAAGCGAACAAGCGGAAATAATAATTTCACTTACCTACATGATATGTATGAAGAAGCCATGAAAGGTGTTAAACATCTTCTTGTTCGGAAGTCTATTCCAAATGGGTTGGTTTTTGTTGGAGAATTGCCCTATGGATCGGATTCTACTATTAGTCCGAAGATGGATCATCTGGTATGTTTCTTATCAGGTACCCTTGCTCTTGGTGCTACCAAGGGCATCACCAAGGAAGAAGCCATGAGTAAAAGCTTGTTGAACTTTGATGATCTGGAAAATCTAAATCTGGCAGAGGATCTTGCAAAAACTTGCTTTGAAATGTATGAAGTAACCTCTACCGGTCTTGCCCCAGAAATTGCTTATTTTCATACTGAGGAATATTCTGAACAAGGCCTTGATGGTGGAAATAAAAATTCGAAATACATCGATGACATAATAATCAAGCCACTGGATCGTCATAATCTTATGCGGCCCGAAACAGTTGAGTCATTGTTTGTCCTGTATCGTATCACAGGCGATCCAAAGTATCGTGCATGGGGGTGGGAGATTTTCGAAGCATTCGAGAGGCACACAAAGGTTAGTAGTGGTGGATATACATCACTAGATGATGTTACTGCGGTTCCTCCTCGGAGAAGGGACAAAATGGAGACATTCTTCCTGGGGGAGACACTCAAGTATTTGTACTTGCTATTTGGGGACAGTTCCACGATCCCATTGGATAAATATGTGTTCAATACTGAAGCACATCCTCTACCAATGGAAGGCAATATTCAGGTTCAATGA

Coding sequence (CDS)

ATGTCGAGTTCTCTACCTTACTCTGTCAAGGATGTTCACTACGACAATGCTAAGTTTCGACAGAGATCCTACGTCAAGGTGATGACTCAGAATTTACTCACTGGTAGCAGAAAACGTGATTATCTAAGTTGTAGCACAGGAAAATTTCTTGTATTACTTTTGATCTTCGGTTTGGCATATCTTTTATTGACAAGTGCTACTCCTGTCCATGTTCCCAACCAACTTGCCAAAGTTAGCGAGAATAGTGAGAAACAAGAGATTAAATATAATGGTGGCATCAACTTCAGGAACTTATGGAGGAAAGCTCCTAGGCTTCCCCCTCAATTACCACCAGATGAAAAAGGTAGCAATACTAGCTATCATATCGATCCAAACACGTTAATCTCTGAGTCTTTATGGATTTCAAGGCAACAGAAAGTAAAGGATGCTTTTATCCATGCATGGTCCGGCTATAAAAGGTATGCAATGGGGTATGATGAACTTATGCCACTGAGCCAGCATGGAACCGATGGGTTAGGAGGTTTAGGTGCTACAGTTGTGGATGCCCTTGACACTGCCATGATAATGGGCGCTAGTGATATTGTCTCTGAGGCAGGCTCATGGATTGAAAAAAACCTTATGGATAGAATTAAGGAAAAAGGACAAGTCAATTTATTTGAAACTACCATACGTGTCCTAGGTGGTCTCTTGAGTGCATATCACTTGAGAGCTGGAGAGTATGGGAAGACCTTTGGAAATGAAGGACCCAAACCTGCTGTTTATCTAGAAATTGCGAAGAACTTGGCTGATCGTTTGCTAAGTGCTTTTACCTCCAGTCCAACTGCTATTCCTCTCTGTGATGTTGTTTTAAAAGATTCCTCTGCCCATGCAGCTCCTGGTGGATCAAGTAGTACATCCGAAGTATCCACTTTGCAGCTGGAATTTAATTATCTTAGTACTGTTTCAGGCGACCCAAAGTATTCAATTGCTGCTATGAAGGTCTTGGAACACATGAAGAGCCTTCCGAAAGTGGAAGGTTTGGTTCCCATCTATATTAGCCCTCATACTGGCGAATTTAGTGGTGAAACCATAAGACTGGGATCTCGTGGTGATAGCTACTACGAGTATCTACTGAAAGTGTGGCTTCAGAAGCGAACAAGCGGAAATAATAATTTCACTTACCTACATGATATGTATGAAGAAGCCATGAAAGGTGTTAAACATCTTCTTGTTCGGAAGTCTATTCCAAATGGGTTGGTTTTTGTTGGAGAATTGCCCTATGGATCGGATTCTACTATTAGTCCGAAGATGGATCATCTGGTATGTTTCTTATCAGGTACCCTTGCTCTTGGTGCTACCAAGGGCATCACCAAGGAAGAAGCCATGAGTAAAAGCTTGTTGAACTTTGATGATCTGGAAAATCTAAATCTGGCAGAGGATCTTGCAAAAACTTGCTTTGAAATGTATGAAGTAACCTCTACCGGTCTTGCCCCAGAAATTGCTTATTTTCATACTGAGGAATATTCTGAACAAGGCCTTGATGGTGGAAATAAAAATTCGAAATACATCGATGACATAATAATCAAGCCACTGGATCGTCATAATCTTATGCGGCCCGAAACAGTTGAGTCATTGTTTGTCCTGTATCGTATCACAGGCGATCCAAAGTATCGTGCATGGGGGTGGGAGATTTTCGAAGCATTCGAGAGGCACACAAAGGTTAGTAGTGGTGGATATACATCACTAGATGATGTTACTGCGGTTCCTCCTCGGAGAAGGGACAAAATGGAGACATTCTTCCTGGGGGAGACACTCAAGTATTTGTACTTGCTATTTGGGGACAGTTCCACGATCCCATTGGATAAATATGTGTTCAATACTGAAGCACATCCTCTACCAATGGAAGGCAATATTCAGGTTCAATGA

Protein sequence

MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAYLLLTSATPVHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQVQ*
Homology
BLAST of Chy2G024160 vs. ExPASy Swiss-Prot
Match: Q93Y37 (Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 OS=Arabidopsis thaliana OX=3702 GN=MNS3 PE=1 SV=1)

HSP 1 Score: 922.5 bits (2383), Expect = 2.5e-267
Identity = 456/634 (71.92%), Postives = 528/634 (83.28%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYSVKD+HYDNAKFR RS +KV +Q+LLT S KR+Y SCSTGKFL+L+L FG+A 
Sbjct: 1   MSKSLPYSVKDIHYDNAKFRHRSPLKVFSQSLLTLSTKRNYASCSTGKFLILILFFGVAC 60

Query: 61  LLLTSATPVHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDE---KGSN 120
           L+L S +P          S  +EK ++ + GG+    L RK PRLPP+L PDE   +GS+
Sbjct: 61  LMLMSKSP--------NESGLNEKGKVTFVGGLRLGGLLRKPPRLPPRLSPDEGQLRGSS 120

Query: 121 TSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGA 180
           T+     N   S+  W +RQQ VK+AF HAWSGY++YAMGYDELMP+SQ G DGLGGLGA
Sbjct: 121 TNGSTISN---SDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLGA 180

Query: 181 TVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRA 240
           TVVDALDTAMIMG  +IVSEAGSW+E +L++RI +KGQVNLFETTIRVLGGLLSAYHL  
Sbjct: 181 TVVDALDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSG 240

Query: 241 GEYGK-TFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSS 300
           GE G     + GPKP +YL IAK+LADRLLSAFTSSPT +P CDV+L +S+AH APGG+S
Sbjct: 241 GEQGTVNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAHPAPGGAS 300

Query: 301 STSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGET 360
           ST+EV+++QLEFNYLS++SGDPKYS  AMKVL H+K+LPK EGLVPIYISP TG+F GE 
Sbjct: 301 STAEVASVQLEFNYLSSISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGEN 360

Query: 361 IRLGSRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVG 420
           IRLGSRGDSYYEYL+KVWLQ+    N+NFTYLHDMY EAMKGV+HLLV+ SIP GLVFVG
Sbjct: 361 IRLGSRGDSYYEYLIKVWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFVG 420

Query: 421 ELPYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAK 480
           ELPYGS    SPKMDHLVCFL GTLALGATKG+TKE+A+ ++LL+F+DLENL LAEDLAK
Sbjct: 421 ELPYGSKGEFSPKMDHLVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLAK 480

Query: 481 TCFEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVE 540
           TCFEMYEVT+TGLAPEIAYFHT++Y+E GLDGGNK+S Y +DIIIKP DRHNL+RPETVE
Sbjct: 481 TCFEMYEVTATGLAPEIAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETVE 540

Query: 541 SLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGE 600
           SLFVLYRIT D KYR  GW+IFEAFE++TKV SGGYTSLDDVT VPP RRDKMETFFLGE
Sbjct: 541 SLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLGE 600

Query: 601 TLKYLYLLFGDSSTIPLDKYVFNTEAHPLPMEGN 631
           TLKYLYLLFGD S IPLDK+VFNTEAHPLP+  N
Sbjct: 601 TLKYLYLLFGDDSVIPLDKFVFNTEAHPLPIRRN 623

BLAST of Chy2G024160 vs. ExPASy Swiss-Prot
Match: Q8J0Q0 (Mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Candida albicans OX=5476 GN=MNS1 PE=3 SV=2)

HSP 1 Score: 406.0 bits (1042), Expect = 7.9e-112
Identity = 241/509 (47.35%), Postives = 312/509 (61.30%), Query Frame = 0

Query: 130 ESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTD-GLGGLGATVVDALDTAMI 189
           +S W   Q +V+ A + +W  Y++Y  GYD   P+ Q G + G   LG  +VD+LDT MI
Sbjct: 32  QSKWERAQSEVRQAILDSWHTYEKYGWGYDVYHPIKQEGENMGPKPLGWMIVDSLDTLMI 91

Query: 190 MGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEG 249
           M   + VS A  WI KN +D   +   VN FETTIR+LGGLLSAYH         F N+ 
Sbjct: 92  MDCPEEVSRARDWI-KNDLDYTFDY-NVNTFETTIRMLGGLLSAYH---------FSNDD 151

Query: 250 PKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSA--HAAPGGSSSTSEVSTLQL 309
               VYL+ A  LA+ L  A+  SP+ IP   V LK      +    G+SST+E +T+QL
Sbjct: 152 ----VYLDKAVQLANALHGAY-DSPSGIPYSSVNLKSGKGIKNHVDNGASSTAEAATVQL 211

Query: 310 EFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSY 369
           E  YLS ++G+  +   A KV++ ++S    +GLVPIY++P TG++ G  IRLGSRGDSY
Sbjct: 212 EMKYLSKLTGEILWWNLAEKVMQVLESNKPQDGLVPIYVNPDTGKYQGHLIRLGSRGDSY 271

Query: 370 YEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTI 429
           YEYLLK +LQ     N       DMY E+++GVK  LV  S P+GL F+GEL  G    +
Sbjct: 272 YEYLLKQYLQT----NKQELVYWDMYRESVEGVKKHLVSDSYPSGLTFIGELDNGIGGKL 331

Query: 430 SPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMY-EVT 489
           S KMDHLVCF  G LALGAT G+T  EA S    N +   +  L E+L  TC++MY +V+
Sbjct: 332 STKMDHLVCFYGGLLALGATGGLTLNEAQSLKSWNEEREADFKLGEELTYTCYKMYHDVS 391

Query: 490 STGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRIT 549
            TGL+PEI  F             N+++    D IIKPLDRHNL RPETVESLF LYR+T
Sbjct: 392 PTGLSPEIVVF-------------NEDTSKSKDFIIKPLDRHNLQRPETVESLFYLYRLT 451

Query: 550 GDPKYRAWGWEIFEAFERHTKV--SSG--GYTSLDDVTAVP----PRRRDKMETFFLGET 609
           GD KYR  G+EIF+ F ++TKV  S G   ++SL DVT+      P+ +D  E+F+  ET
Sbjct: 452 GDVKYREMGYEIFQNFIKYTKVVNSEGEVSFSSLSDVTSFDSNGLPKFKDNTESFWWAET 507

Query: 610 LKYLYLLFGDSSTIPLDKYVFNTEAHPLP 627
           LKYLYLLF D++ IPL  YVFNTEAHP P
Sbjct: 512 LKYLYLLFDDTNKIPLTDYVFNTEAHPFP 507

BLAST of Chy2G024160 vs. ExPASy Swiss-Prot
Match: Q9UKM7 (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Homo sapiens OX=9606 GN=MAN1B1 PE=1 SV=2)

HSP 1 Score: 401.7 bits (1031), Expect = 1.5e-110
Identity = 238/532 (44.74%), Postives = 323/532 (60.71%), Query Frame = 0

Query: 101 KAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDE 160
           + P  PP LPP  +   T  H++            RQ+ V D F+HAW GY+++A G+DE
Sbjct: 227 EVPTKPP-LPP-ARTQGTPVHLN-----------YRQKGVIDVFLHAWKGYRKFAWGHDE 286

Query: 161 LMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFE 220
           L P+S+  ++   GLG T++DALDT  I+G      EA  W+ K L    ++   VNLFE
Sbjct: 287 LKPVSRSFSEWF-GLGLTLIDALDTMWILGLRKEFEEARKWVSKKL--HFEKDVDVNLFE 346

Query: 221 TTIRVLGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCD 280
           +TIR+LGGLLSAYHL                +++L  A++  +RL+ AF  +P+ IP  D
Sbjct: 347 STIRILGGLLSAYHLSG-------------DSLFLRKAEDFGNRLMPAF-RTPSKIPYSD 406

Query: 281 VVLKDSSAHAAPGGSSST-SEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLP-KVE 340
           V +    AH     S ST +EV+++QLEF  LS ++GD K+  A  KV +H+  L  K +
Sbjct: 407 VNIGTGVAHPPRWTSDSTVAEVTSIQLEFRELSRLTGDKKFQEAVEKVTQHIHGLSGKKD 466

Query: 341 GLVPIYISPHTGEFSG-ETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMK 400
           GLVP++I+ H+G F+      LG+R DSYYEYLLK W+Q    G    T L + Y EA++
Sbjct: 467 GLVPMFINTHSGLFTHLGVFTLGARADSYYEYLLKQWIQ----GGKQETQLLEDYVEAIE 526

Query: 401 GVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSK 460
           GV+  L+R S P+ L FVGEL +G     S KMDHLVCFL GTLALG   G+        
Sbjct: 527 GVRTHLLRHSEPSKLTFVGELAHG---RFSAKMDHLVCFLPGTLALGVYHGL-------- 586

Query: 461 SLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYID 520
                    ++ LA++L +TC++M     TGL+PEI +F+   Y + G            
Sbjct: 587 ------PASHMELAQELMETCYQMNRQMETGLSPEIVHFNL--YPQPGR----------R 646

Query: 521 DIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDD 580
           D+ +KP DRHNL+RPETVESLF LYR+TGD KY+ WGWEI ++F R T+V SGGY+S+++
Sbjct: 647 DVEVKPADRHNLLRPETVESLFYLYRVTGDRKYQDWGWEILQSFSRFTRVPSGGYSSINN 695

Query: 581 V-TAVPPRRRDKMETFFLGETLKYLYLLFGDS-STIPLDKYVFNTEAHPLPM 628
           V     P  RDKME+FFLGETLKYL+LLF D  + + LD YVFNTEAHPLP+
Sbjct: 707 VQDPQKPEPRDKMESFFLGETLKYLFLLFSDDPNLLSLDAYVFNTEAHPLPI 695

BLAST of Chy2G024160 vs. ExPASy Swiss-Prot
Match: B2GUY0 (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Rattus norvegicus OX=10116 GN=Man1b1 PE=2 SV=2)

HSP 1 Score: 395.2 bits (1014), Expect = 1.4e-108
Identity = 235/563 (41.74%), Postives = 336/563 (59.68%), Query Frame = 0

Query: 76  AKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLW-- 135
           A+  EN+++  I + G +           + P+   +         I P  L S  +W  
Sbjct: 153 AQQEENTQRTVISWRGAV-----------IEPEQATEPPSKRAEASIKPLFLASR-IWKE 212

Query: 136 ----ISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIM 195
                 RQ+ V +AF+HAW GY+++A G+DEL P+S+  ++   GLG T++DALDT  I+
Sbjct: 213 PAPPNERQKGVIEAFLHAWKGYQKFAWGHDELKPVSKTFSEWF-GLGLTLIDALDTMWIL 272

Query: 196 GASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEGP 255
           G      EA  W+ +NL    ++   VNLFE+TIR+LGGLLSAYHL              
Sbjct: 273 GLKQEFKEARKWVSENL--DFQKNVDVNLFESTIRILGGLLSAYHLSG------------ 332

Query: 256 KPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSST-SEVSTLQLEF 315
             +++L  A++  +RL+ AFT +P+ IP  DV +    AH+    S ST +EV+++QLEF
Sbjct: 333 -DSLFLSKAEDFGNRLMPAFT-TPSKIPYSDVNIGTGFAHSPQWTSDSTVAEVTSIQLEF 392

Query: 316 NYLSTVSGDPKYSIAAMKVLEHMKSLP-KVEGLVPIYISPHTGEFSGETI-RLGSRGDSY 375
             LS ++G  K+  A  +V +H+ SL  K +GLVP++I+ ++G F+   +  LG+R DSY
Sbjct: 393 RELSRLTGIKKFQEAVEEVTKHIHSLSGKKDGLVPMFINTNSGLFTHPGVFTLGARADSY 452

Query: 376 YEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTI 435
           YEYLLK W+Q    G    T L + Y  A++G+K  L+R+S P  L FVGEL +G     
Sbjct: 453 YEYLLKQWIQ----GGKKETQLLEDYVRAIEGIKAHLLRQSQPRKLTFVGELAHG---RF 512

Query: 436 SPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTS 495
           S KMDHLVCFL GTLALG   G+                ++++LA  L +TC++M +   
Sbjct: 513 SAKMDHLVCFLPGTLALGVHHGL--------------PADHMDLARALMETCYQMNQQME 572

Query: 496 TGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITG 555
           TGL+PEIA+F+    ++              D+ +KP DRHNL+RPETVESLF LYR+T 
Sbjct: 573 TGLSPEIAHFNMYPRADH------------KDVEVKPADRHNLLRPETVESLFYLYRVTK 632

Query: 556 DPKYRAWGWEIFEAFERHTKVSSGGYTSLDDV-TAVPPRRRDKMETFFLGETLKYLYLLF 615
           D KY+ WGWEI ++F ++T+V SGGY+S+++V  +  P  RDKME+FF+GETLKYLYLLF
Sbjct: 633 DRKYQDWGWEILQSFNKYTRVPSGGYSSINNVQNSHKPEPRDKMESFFVGETLKYLYLLF 653

Query: 616 GDS-STIPLDKYVFNTEAHPLPM 628
            D    + LD  VFNTEAHPLP+
Sbjct: 693 SDDLELLGLDTCVFNTEAHPLPI 653

BLAST of Chy2G024160 vs. ExPASy Swiss-Prot
Match: A2AJ15 (Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Mus musculus OX=10090 GN=Man1b1 PE=1 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.5e-107
Identity = 233/563 (41.39%), Postives = 336/563 (59.68%), Query Frame = 0

Query: 76  AKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLW-- 135
           A+  EN+++  I + G +           + P+   +         I P  L S+ +W  
Sbjct: 154 AQQEENTQRTVISWRGAV-----------IEPEQATELPYKRAEASIKPLVLASK-IWKE 213

Query: 136 ----ISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIM 195
                 RQ+ V +AF+HAW GY+++A G+DEL P+S+  ++   GLG T++DALDT  I+
Sbjct: 214 PAPPNERQKGVIEAFLHAWKGYQKFAWGHDELKPVSKTFSEWF-GLGLTLIDALDTMWIL 273

Query: 196 GASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEGP 255
           G      +A  W+ +NL    ++   VNLFE+TIR+LGGLLS YHL              
Sbjct: 274 GLKQEFKQARKWVSENL--DFQKNVDVNLFESTIRILGGLLSTYHLSG------------ 333

Query: 256 KPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSST-SEVSTLQLEF 315
             +++L  A++   RL+ AFT +P+ IP  DV +    AH+    S ST +EV+++QLEF
Sbjct: 334 -DSLFLTKAEDFGKRLMPAFT-TPSKIPYSDVNIGTGFAHSPQWTSDSTVAEVTSIQLEF 393

Query: 316 NYLSTVSGDPKYSIAAMKVLEHMKSLP-KVEGLVPIYISPHTGEFSGETI-RLGSRGDSY 375
             LS ++G  K+  A  +V +H+ SL  K +GLVP++I+ ++G F+   +  LG+R DSY
Sbjct: 394 RELSRLTGIKKFQEAVEEVTKHIHSLSGKKDGLVPMFINTNSGLFTHPGVFTLGARADSY 453

Query: 376 YEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTI 435
           YEYLLK W+Q    G    T L + Y +A++G+K  L+R+S P  L FVGEL +G     
Sbjct: 454 YEYLLKQWIQ----GGKKETQLLEDYVKAIEGIKAHLLRQSQPRKLTFVGELAHG---RF 513

Query: 436 SPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTS 495
           S KMDHLVCFL GTLALG   G+                ++++LA  L +TC++M +   
Sbjct: 514 SAKMDHLVCFLPGTLALGVHHGL--------------PADHMDLARALMETCYQMNQQME 573

Query: 496 TGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITG 555
           TGL+PEIA+F+    ++              D+ +KP DRHNL+RPETVESLF LYR+T 
Sbjct: 574 TGLSPEIAHFNMYPRADH------------KDVEVKPADRHNLLRPETVESLFYLYRVTR 633

Query: 556 DPKYRAWGWEIFEAFERHTKVSSGGYTSLDDV-TAVPPRRRDKMETFFLGETLKYLYLLF 615
           D KY+ WGWEI ++F ++T+V SGGY+S+++V  +  P  RDKME+FF+GETLKYLYLLF
Sbjct: 634 DRKYQDWGWEILQSFNKYTRVPSGGYSSINNVQNSHKPEPRDKMESFFVGETLKYLYLLF 654

Query: 616 GDS-STIPLDKYVFNTEAHPLPM 628
            D    + LD  VFNTEAHPLP+
Sbjct: 694 SDDLELLSLDSCVFNTEAHPLPI 654

BLAST of Chy2G024160 vs. ExPASy TrEMBL
Match: A0A0A0KLY9 (alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G523340 PE=3 SV=1)

HSP 1 Score: 1266.1 bits (3275), Expect = 0.0e+00
Identity = 623/634 (98.26%), Postives = 631/634 (99.53%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSATPVHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120
           LLLTSATPVHVPN+LAKVSENSEKQEIK NGGINFRNLWRKAPRLPPQLPPDEKGSNTSY
Sbjct: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180
           HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIR++GGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240

Query: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTG+FSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420
           SRGDSYYEYLLKVWLQKRTSGN+NFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480
           GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE
Sbjct: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNK SKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKY 600
           LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVT +PPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQVQ 635
           LYLLFGDSSTIPLDKYVFNTEAHPLPM+GNIQ+Q
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 634

BLAST of Chy2G024160 vs. ExPASy TrEMBL
Match: A0A1S3AZ69 (alpha-1,2-Mannosidase OS=Cucumis melo OX=3656 GN=LOC103484372 PE=3 SV=1)

HSP 1 Score: 1256.1 bits (3249), Expect = 0.0e+00
Identity = 618/634 (97.48%), Postives = 629/634 (99.21%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRSY+KVMTQNLLTGSRKRDYLSCSTGKFL+LL+IFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYLKVMTQNLLTGSRKRDYLSCSTGKFLILLMIFGLAY 60

Query: 61  LLLTSATPVHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120
           LLLT+ATPVHVPNQ AKVSEN+EK+EIK NGGINFRNLWRKAPRLPPQLPPDEKGSNTSY
Sbjct: 61  LLLTNATPVHVPNQPAKVSENNEKREIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180
           HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSWIEK+LMDRIKEKGQVNLFETTIRV+GGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKHLMDRIKEKGQVNLFETTIRVVGGLLSAYHLRAGEY 240

Query: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420
           SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480
           GSDS ISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLL+FDDLENLNLAEDLAKTCFE
Sbjct: 421 GSDSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEIAYFHTEEYSEQG DGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGHDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKY 600
           LYRITG+PKYRAWGWEIFEAFERHTKVSSGGYTSLDDVT VPPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGNPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQVQ 635
           LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQ+Q
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQIQ 634

BLAST of Chy2G024160 vs. ExPASy TrEMBL
Match: A0A5A7UEF9 (alpha-1,2-Mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G008980 PE=3 SV=1)

HSP 1 Score: 1204.5 bits (3115), Expect = 0.0e+00
Identity = 592/607 (97.53%), Postives = 602/607 (99.18%), Query Frame = 0

Query: 28  MTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAYLLLTSATPVHVPNQLAKVSENSEKQEI 87
           MTQNLLTGSRKRDYLSCSTGKFL+LL+IFGLAYLLLT+ATPVHVPNQ AKVSEN+EK+EI
Sbjct: 1   MTQNLLTGSRKRDYLSCSTGKFLILLMIFGLAYLLLTNATPVHVPNQPAKVSENNEKREI 60

Query: 88  KYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHA 147
           K NGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHA
Sbjct: 61  KNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHA 120

Query: 148 WSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKNLM 207
           WSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEK+LM
Sbjct: 121 WSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKHLM 180

Query: 208 DRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLS 267
           DRIKEKGQVNLFETTIRV+GGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLS
Sbjct: 181 DRIKEKGQVNLFETTIRVVGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLS 240

Query: 268 AFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKV 327
           AFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKV
Sbjct: 241 AFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKV 300

Query: 328 LEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFTY 387
           LEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFTY
Sbjct: 301 LEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFTY 360

Query: 388 LHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMDHLVCFLSGTLALGATK 447
           LHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDS ISPKMDHLVCFLSGTLALGATK
Sbjct: 361 LHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSAISPKMDHLVCFLSGTLALGATK 420

Query: 448 GITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGLD 507
           GITKEEAMSKSLL+FDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQG D
Sbjct: 421 GITKEEAMSKSLLSFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGHD 480

Query: 508 GGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRAWGWEIFEAFERHTKV 567
           GGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITG+PKYRAWGWEIFEAFERHTKV
Sbjct: 481 GGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNPKYRAWGWEIFEAFERHTKV 540

Query: 568 SSGGYTSLDDVTAVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPM 627
           SSGGYTSLDDVT VPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPM
Sbjct: 541 SSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPM 600

Query: 628 EGNIQVQ 635
           EGNIQ+Q
Sbjct: 601 EGNIQIQ 607

BLAST of Chy2G024160 vs. ExPASy TrEMBL
Match: A0A6J1KW66 (alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111496964 PE=3 SV=1)

HSP 1 Score: 1166.8 bits (3017), Expect = 0.0e+00
Identity = 574/633 (90.68%), Postives = 599/633 (94.63%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           M SSLPYSVKDVHYDNAKFRQRSY KVMTQNL TGSRKRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLFTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSATP-VHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTS 120
           LLLTS TP   VPNQL KV EN++K E K NGGINFRNLWRK PRLPP+LPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNQLDKVRENNQKGEFKNNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATV 180
           YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMG+DELMPLSQ GTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMGASDIVSEAGSW+EK+L+DRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 YGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300
           YGKTF NEGPKPAVYLE AKNLADRLLSAFTSSPTAIPLCDVVL+DSSAHAAPGG SSTS
Sbjct: 241 YGKTFENEGPKPAVYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYSIAAMKVLEHMKSLPK+EGLVPIYISP +G+FSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSIAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQK    NNN TYLHDMYEEAMKGVKHLLVRKS+PNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENNNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCF 480
           YGS   ISPKMDHLVCFL GTLALGATKGI+KEE+M KSLL+F+DLENLNLAEDLAKTCF
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEESMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLF 540
           EMYEVT+TGLAPEI YFHTEEYSE G DGGNKN+KYIDDIIIKP DRHNL+RPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLK 600
           VLYRITGDPKYRAWGW+IFEAFERHTKV++GGYTSLDDVT VPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQ 633
           YLYLLFGD+STIPLDKYVFNTEAHPLP+E NIQ
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIETNIQ 633

BLAST of Chy2G024160 vs. ExPASy TrEMBL
Match: A0A6J1ELX6 (alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111433756 PE=3 SV=1)

HSP 1 Score: 1164.1 bits (3010), Expect = 0.0e+00
Identity = 573/633 (90.52%), Postives = 599/633 (94.63%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           M SSLPYSVKDVHYDNAKFRQRSY KVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MRSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSATP-VHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTS 120
           LLLTS TP   VPN+L KVSEN++K E K NGGINFRNLWRK PRLPP+LPPDEKGSNTS
Sbjct: 61  LLLTSTTPGDFVPNRLDKVSENNQKGEFKSNGGINFRNLWRKPPRLPPRLPPDEKGSNTS 120

Query: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATV 180
           YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMG+DELMPLSQ GTDGLGGLGATV
Sbjct: 121 YHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGHDELMPLSQQGTDGLGGLGATV 180

Query: 181 VDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240
           VDALDTAMIMGASDIVSEAGSW+EK+L+DRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE
Sbjct: 181 VDALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGE 240

Query: 241 YGKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTS 300
           YGKTF NEGP PA YLE AKNLADRLLSAFTSSPTAIPLCDVVL+DSSAHAAPGG SSTS
Sbjct: 241 YGKTFENEGPTPAAYLETAKNLADRLLSAFTSSPTAIPLCDVVLRDSSAHAAPGGLSSTS 300

Query: 301 EVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRL 360
           EVSTLQLEFNYLST+SGDPKYSIAAMKVLEHMKSLPK+EGLVPIYISP +G+FSGETIRL
Sbjct: 301 EVSTLQLEFNYLSTISGDPKYSIAAMKVLEHMKSLPKLEGLVPIYISPDSGQFSGETIRL 360

Query: 361 GSRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELP 420
           GSRGDSYYEYLLKVWLQK    N+N TYLHDMYEEAMKGVKHLLVRKS+PNGLVFVGELP
Sbjct: 361 GSRGDSYYEYLLKVWLQKGARENHNLTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELP 420

Query: 421 YGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCF 480
           YGS   ISPKMDHLVCFL GTLALGATKGI+KEEAM KSLL+F+DLENLNLAEDLAKTCF
Sbjct: 421 YGSSEAISPKMDHLVCFLPGTLALGATKGISKEEAMKKSLLSFEDLENLNLAEDLAKTCF 480

Query: 481 EMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLF 540
           EMYEVT+TGLAPEI YFHTEEYSE G DGGNKN+KYIDDIIIKP DRHNL+RPETVESLF
Sbjct: 481 EMYEVTATGLAPEIVYFHTEEYSEGGHDGGNKNAKYIDDIIIKPPDRHNLLRPETVESLF 540

Query: 541 VLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLK 600
           VLYRITGDPKYRAWGW+IFEAFERHTKV++GGYTSLDDVT VPPRRRDKMETFFLGETLK
Sbjct: 541 VLYRITGDPKYRAWGWQIFEAFERHTKVNTGGYTSLDDVTTVPPRRRDKMETFFLGETLK 600

Query: 601 YLYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQ 633
           YLYLLFGD+STIPLDKYVFNTEAHPLP+E NIQ
Sbjct: 601 YLYLLFGDNSTIPLDKYVFNTEAHPLPIETNIQ 633

BLAST of Chy2G024160 vs. NCBI nr
Match: XP_004134582.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis sativus] >KGN49392.1 hypothetical protein Csa_002711 [Cucumis sativus])

HSP 1 Score: 1257 bits (3253), Expect = 0.0
Identity = 623/634 (98.26%), Postives = 631/634 (99.53%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSATPVHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120
           LLLTSATPVHVPN+LAKVSENSEKQEIK NGGINFRNLWRKAPRLPPQLPPDEKGSNTSY
Sbjct: 61  LLLTSATPVHVPNKLAKVSENSEKQEIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180
           HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIR++GGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRIVGGLLSAYHLRAGEY 240

Query: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTG+FSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGKFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420
           SRGDSYYEYLLKVWLQKRTSGN+NFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNSNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480
           GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE
Sbjct: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNK SKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKKSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKY 600
           LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVT +PPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTIPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQVQ 634
           LYLLFGDSSTIPLDKYVFNTEAHPLPM+GNIQ+Q
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMDGNIQIQ 634

BLAST of Chy2G024160 vs. NCBI nr
Match: XP_008439639.1 (PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis melo])

HSP 1 Score: 1247 bits (3227), Expect = 0.0
Identity = 618/634 (97.48%), Postives = 629/634 (99.21%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRSY+KVMTQNLLTGSRKRDYLSCSTGKFL+LL+IFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYLKVMTQNLLTGSRKRDYLSCSTGKFLILLMIFGLAY 60

Query: 61  LLLTSATPVHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120
           LLLT+ATPVHVPNQ AKVSEN+EK+EIK NGGINFRNLWRKAPRLPPQLPPDEKGSNTSY
Sbjct: 61  LLLTNATPVHVPNQPAKVSENNEKREIKNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120

Query: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180
           HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV
Sbjct: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSWIEK+LMDRIKEKGQVNLFETTIRV+GGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWIEKHLMDRIKEKGQVNLFETTIRVVGGLLSAYHLRAGEY 240

Query: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420
           SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420

Query: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480
           GSDS ISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLL+FDDLENLNLAEDLAKTCFE
Sbjct: 421 GSDSAISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCFE 480

Query: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEIAYFHTEEYSEQG DGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIAYFHTEEYSEQGHDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKY 600
           LYRITG+PKYRAWGWEIFEAFERHTKVSSGGYTSLDDVT VPPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGNPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQVQ 634
           LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQ+Q
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQIQ 634

BLAST of Chy2G024160 vs. NCBI nr
Match: XP_038882608.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X1 [Benincasa hispida])

HSP 1 Score: 1213 bits (3138), Expect = 0.0
Identity = 600/634 (94.64%), Postives = 617/634 (97.32%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MSSSLPYSVKDVHYDNAKFRQRSY KVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY
Sbjct: 1   MSSSLPYSVKDVHYDNAKFRQRSYFKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60

Query: 61  LLLTSATPVHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSY 120
           LLLTS TPVH PN++AKV EN+EK E K NGGINFRNLWR+APRLPP+LPPDEKGSNTSY
Sbjct: 61  LLLTSTTPVHGPNRVAKVGENNEKGEFKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSY 120

Query: 121 HIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180
           H DPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV
Sbjct: 121 HFDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVV 180

Query: 181 DALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240
           DALDTAMIMGASDIVSEAGSW+EK+L+DRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY
Sbjct: 181 DALDTAMIMGASDIVSEAGSWVEKHLLDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEY 240

Query: 241 GKTFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300
           GK F NEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE
Sbjct: 241 GKRFENEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSE 300

Query: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLG 360
           VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISP TG+FSGETIRLG
Sbjct: 301 VSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPQTGQFSGETIRLG 360

Query: 361 SRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPY 420
           SRGDSYYEYLLKVWLQKR   NNNFTYLHDMYEEAMKGVKHLLVRKS+PNGLVFVGELPY
Sbjct: 361 SRGDSYYEYLLKVWLQKRRRENNNFTYLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELPY 420

Query: 421 GSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFE 480
           GSDS+ISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLL+FDDLENLNLAEDLAKTC+E
Sbjct: 421 GSDSSISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYE 480

Query: 481 MYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFV 540
           MYEVTSTGLAPEI YFHTEEYSEQGLDGGNKNSKYI DIIIKPLDRHNLMRPETVESLFV
Sbjct: 481 MYEVTSTGLAPEIVYFHTEEYSEQGLDGGNKNSKYIGDIIIKPLDRHNLMRPETVESLFV 540

Query: 541 LYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKY 600
           LYRITG+PKYRAWGWEIFEAFERHTKVSSGGYTSLDDVT VPPRRRDKMETFFLGETLKY
Sbjct: 541 LYRITGNPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKY 600

Query: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPMEGNIQVQ 634
           LYLLFGDSSTIPLDKYVFNTEAHPLP+EGNIQ++
Sbjct: 601 LYLLFGDSSTIPLDKYVFNTEAHPLPIEGNIQIK 634

BLAST of Chy2G024160 vs. NCBI nr
Match: KAA0052546.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis melo var. makuwa] >TYK13279.1 mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis melo var. makuwa])

HSP 1 Score: 1195 bits (3091), Expect = 0.0
Identity = 592/607 (97.53%), Postives = 602/607 (99.18%), Query Frame = 0

Query: 28  MTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAYLLLTSATPVHVPNQLAKVSENSEKQEI 87
           MTQNLLTGSRKRDYLSCSTGKFL+LL+IFGLAYLLLT+ATPVHVPNQ AKVSEN+EK+EI
Sbjct: 1   MTQNLLTGSRKRDYLSCSTGKFLILLMIFGLAYLLLTNATPVHVPNQPAKVSENNEKREI 60

Query: 88  KYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHA 147
           K NGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHA
Sbjct: 61  KNNGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIHA 120

Query: 148 WSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKNLM 207
           WSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEK+LM
Sbjct: 121 WSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKHLM 180

Query: 208 DRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLS 267
           DRIKEKGQVNLFETTIRV+GGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLS
Sbjct: 181 DRIKEKGQVNLFETTIRVVGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLLS 240

Query: 268 AFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKV 327
           AFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKV
Sbjct: 241 AFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMKV 300

Query: 328 LEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFTY 387
           LEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFTY
Sbjct: 301 LEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFTY 360

Query: 388 LHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMDHLVCFLSGTLALGATK 447
           LHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDS ISPKMDHLVCFLSGTLALGATK
Sbjct: 361 LHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSAISPKMDHLVCFLSGTLALGATK 420

Query: 448 GITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGLD 507
           GITKEEAMSKSLL+FDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQG D
Sbjct: 421 GITKEEAMSKSLLSFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGHD 480

Query: 508 GGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRAWGWEIFEAFERHTKV 567
           GGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITG+PKYRAWGWEIFEAFERHTKV
Sbjct: 481 GGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGNPKYRAWGWEIFEAFERHTKV 540

Query: 568 SSGGYTSLDDVTAVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPM 627
           SSGGYTSLDDVT VPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPM
Sbjct: 541 SSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLPM 600

Query: 628 EGNIQVQ 634
           EGNIQ+Q
Sbjct: 601 EGNIQIQ 607

BLAST of Chy2G024160 vs. NCBI nr
Match: XP_038882609.1 (mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X2 [Benincasa hispida])

HSP 1 Score: 1163 bits (3008), Expect = 0.0
Identity = 575/608 (94.57%), Postives = 592/608 (97.37%), Query Frame = 0

Query: 27  VMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAYLLLTSATPVHVPNQLAKVSENSEKQE 86
           VMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAYLLLTS TPVH PN++AKV EN+EK E
Sbjct: 20  VMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAYLLLTSTTPVHGPNRVAKVGENNEKGE 79

Query: 87  IKYNGGINFRNLWRKAPRLPPQLPPDEKGSNTSYHIDPNTLISESLWISRQQKVKDAFIH 146
            K NGGINFRNLWR+APRLPP+LPPDEKGSNTSYH DPNTLISESLWISRQQKVKDAFIH
Sbjct: 80  FKNNGGINFRNLWRRAPRLPPRLPPDEKGSNTSYHFDPNTLISESLWISRQQKVKDAFIH 139

Query: 147 AWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEKNL 206
           AWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSW+EK+L
Sbjct: 140 AWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWVEKHL 199

Query: 207 MDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADRLL 266
           +DRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGK F NEGPKPAVYLEIAKNLADRLL
Sbjct: 200 LDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKRFENEGPKPAVYLEIAKNLADRLL 259

Query: 267 SAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMK 326
           SAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMK
Sbjct: 260 SAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYLSTVSGDPKYSIAAMK 319

Query: 327 VLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQKRTSGNNNFT 386
           VLEHMKSLPKVEGLVPIYISP TG+FSGETIRLGSRGDSYYEYLLKVWLQKR   NNNFT
Sbjct: 320 VLEHMKSLPKVEGLVPIYISPQTGQFSGETIRLGSRGDSYYEYLLKVWLQKRRRENNNFT 379

Query: 387 YLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMDHLVCFLSGTLALGAT 446
           YLHDMYEEAMKGVKHLLVRKS+PNGLVFVGELPYGSDS+ISPKMDHLVCFLSGTLALGAT
Sbjct: 380 YLHDMYEEAMKGVKHLLVRKSVPNGLVFVGELPYGSDSSISPKMDHLVCFLSGTLALGAT 439

Query: 447 KGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEEYSEQGL 506
           KGITKEEAMSKSLL+FDDLENLNLAEDLAKTC+EMYEVTSTGLAPEI YFHTEEYSEQGL
Sbjct: 440 KGITKEEAMSKSLLSFDDLENLNLAEDLAKTCYEMYEVTSTGLAPEIVYFHTEEYSEQGL 499

Query: 507 DGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRAWGWEIFEAFERHTK 566
           DGGNKNSKYI DIIIKPLDRHNLMRPETVESLFVLYRITG+PKYRAWGWEIFEAFERHTK
Sbjct: 500 DGGNKNSKYIGDIIIKPLDRHNLMRPETVESLFVLYRITGNPKYRAWGWEIFEAFERHTK 559

Query: 567 VSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLP 626
           VSSGGYTSLDDVT VPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLP
Sbjct: 560 VSSGGYTSLDDVTTVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNTEAHPLP 619

Query: 627 MEGNIQVQ 634
           +EGNIQ++
Sbjct: 620 IEGNIQIK 627

BLAST of Chy2G024160 vs. TAIR 10
Match: AT1G30000.1 (alpha-mannosidase 3 )

HSP 1 Score: 922.5 bits (2383), Expect = 1.8e-268
Identity = 456/634 (71.92%), Postives = 528/634 (83.28%), Query Frame = 0

Query: 1   MSSSLPYSVKDVHYDNAKFRQRSYVKVMTQNLLTGSRKRDYLSCSTGKFLVLLLIFGLAY 60
           MS SLPYSVKD+HYDNAKFR RS +KV +Q+LLT S KR+Y SCSTGKFL+L+L FG+A 
Sbjct: 1   MSKSLPYSVKDIHYDNAKFRHRSPLKVFSQSLLTLSTKRNYASCSTGKFLILILFFGVAC 60

Query: 61  LLLTSATPVHVPNQLAKVSENSEKQEIKYNGGINFRNLWRKAPRLPPQLPPDE---KGSN 120
           L+L S +P          S  +EK ++ + GG+    L RK PRLPP+L PDE   +GS+
Sbjct: 61  LMLMSKSP--------NESGLNEKGKVTFVGGLRLGGLLRKPPRLPPRLSPDEGQLRGSS 120

Query: 121 TSYHIDPNTLISESLWISRQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGA 180
           T+     N   S+  W +RQQ VK+AF HAWSGY++YAMGYDELMP+SQ G DGLGGLGA
Sbjct: 121 TNGSTISN---SDPKWAARQQSVKEAFDHAWSGYRKYAMGYDELMPISQKGVDGLGGLGA 180

Query: 181 TVVDALDTAMIMGASDIVSEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRA 240
           TVVDALDTAMIMG  +IVSEAGSW+E +L++RI +KGQVNLFETTIRVLGGLLSAYHL  
Sbjct: 181 TVVDALDTAMIMGLDNIVSEAGSWVETHLLERISQKGQVNLFETTIRVLGGLLSAYHLSG 240

Query: 241 GEYGK-TFGNEGPKPAVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSS 300
           GE G     + GPKP +YL IAK+LADRLLSAFTSSPT +P CDV+L +S+AH APGG+S
Sbjct: 241 GEQGTVNMTHVGPKPVIYLNIAKDLADRLLSAFTSSPTPVPFCDVILHESTAHPAPGGAS 300

Query: 301 STSEVSTLQLEFNYLSTVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGET 360
           ST+EV+++QLEFNYLS++SGDPKYS  AMKVL H+K+LPK EGLVPIYISP TG+F GE 
Sbjct: 301 STAEVASVQLEFNYLSSISGDPKYSTEAMKVLAHIKTLPKTEGLVPIYISPQTGDFVGEN 360

Query: 361 IRLGSRGDSYYEYLLKVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVG 420
           IRLGSRGDSYYEYL+KVWLQ+    N+NFTYLHDMY EAMKGV+HLLV+ SIP GLVFVG
Sbjct: 361 IRLGSRGDSYYEYLIKVWLQQGAKLNSNFTYLHDMYIEAMKGVRHLLVQNSIPKGLVFVG 420

Query: 421 ELPYGSDSTISPKMDHLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAK 480
           ELPYGS    SPKMDHLVCFL GTLALGATKG+TKE+A+ ++LL+F+DLENL LAEDLAK
Sbjct: 421 ELPYGSKGEFSPKMDHLVCFLPGTLALGATKGLTKEQALKENLLSFEDLENLKLAEDLAK 480

Query: 481 TCFEMYEVTSTGLAPEIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVE 540
           TCFEMYEVT+TGLAPEIAYFHT++Y+E GLDGGNK+S Y +DIIIKP DRHNL+RPETVE
Sbjct: 481 TCFEMYEVTATGLAPEIAYFHTKDYTEDGLDGGNKSSMYANDIIIKPADRHNLLRPETVE 540

Query: 541 SLFVLYRITGDPKYRAWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGE 600
           SLFVLYRIT D KYR  GW+IFEAFE++TKV SGGYTSLDDVT VPP RRDKMETFFLGE
Sbjct: 541 SLFVLYRITKDTKYRDQGWQIFEAFEKYTKVKSGGYTSLDDVTEVPPHRRDKMETFFLGE 600

Query: 601 TLKYLYLLFGDSSTIPLDKYVFNTEAHPLPMEGN 631
           TLKYLYLLFGD S IPLDK+VFNTEAHPLP+  N
Sbjct: 601 TLKYLYLLFGDDSVIPLDKFVFNTEAHPLPIRRN 623

BLAST of Chy2G024160 vs. TAIR 10
Match: AT1G51590.1 (alpha-mannosidase 1 )

HSP 1 Score: 352.4 bits (903), Expect = 7.4e-97
Identity = 218/494 (44.13%), Postives = 296/494 (59.92%), Query Frame = 0

Query: 136 RQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIV 195
           R+QKVK+A IHAWS Y++YA G DEL P ++ GTD  GGLGAT+VD+LDT  IMG  +  
Sbjct: 97  RRQKVKEAMIHAWSSYEKYAWGKDELQPRTKDGTDSFGGLGATMVDSLDTLYIMGLDEQF 156

Query: 196 SEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEGPKPAVYL 255
            +A  W+  +L     +    ++FETTIRV+GGLLSAY L            G K  ++L
Sbjct: 157 QKAREWVASSL--DFDKDYDASMFETTIRVVGGLLSAYDL-----------SGDK--MFL 216

Query: 256 EIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHA---APGGSSSTSEVSTLQLEFNYLS 315
           E AK++ADRLL A+ ++PT IP   + L++ +AH    A GG S  ++  T QLEF  LS
Sbjct: 217 EKAKDIADRLLPAW-NTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALS 276

Query: 316 TVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLK 375
             +GDPKY     KV+  +      +GL+PIYI+P     S  T   G+ GDS+YEYLLK
Sbjct: 277 QRTGDPKYQQKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLLK 336

Query: 376 VWLQ-KRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMD 435
           VW+Q  +TS    +    DM+E++MKG+   LV+KS P+   ++ E    + + +  KMD
Sbjct: 337 VWVQGNKTSAVKPY---RDMWEKSMKGLLS-LVKKSTPSSFTYICE---KNGNNLIDKMD 396

Query: 436 HLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAP 495
            L CF  G LALGA+ G   +E          + + L+LA +LA TC+  Y+ T T LA 
Sbjct: 397 ELACFAPGMLALGAS-GYGPDE----------EKKFLSLAGELAWTCYNFYQSTPTKLAG 456

Query: 496 EIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYR 555
           E  YF T   + Q +  G                  N++RPETVESLF L+R+TG+  Y+
Sbjct: 457 E-NYFFT---AGQDMSVGTS---------------WNILRPETVESLFYLWRLTGNKTYQ 516

Query: 556 AWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKYLYLLFGDSSTI 615
            WGW IF+AFE++++V S GY  L DV      + +KM++FFL ETLKYLYLLF  SS I
Sbjct: 517 EWGWNIFQAFEKNSRVES-GYVGLKDVNT--GAKDNKMQSFFLAETLKYLYLLFSPSSVI 534

Query: 616 PLDKYVFNTEAHPL 626
            LD++VFNTEAHPL
Sbjct: 577 SLDEWVFNTEAHPL 534

BLAST of Chy2G024160 vs. TAIR 10
Match: AT3G21160.1 (alpha-mannosidase 2 )

HSP 1 Score: 345.9 bits (886), Expect = 6.9e-95
Identity = 215/498 (43.17%), Postives = 291/498 (58.43%), Query Frame = 0

Query: 136 RQQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIV 195
           R Q+VK+A +HAWS Y++YA G DEL P ++ G D  GGLGAT++DALDT  IMG  +  
Sbjct: 98  RMQRVKEAMVHAWSSYEKYAWGQDELQPQTKDGVDSFGGLGATMIDALDTLYIMGLDEQF 157

Query: 196 SEAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEGPKPAVYL 255
            +A  W+  +L D  K+    ++FETTIRV+GGLLSAY L            G K  ++L
Sbjct: 158 QKAREWVASSL-DFDKDYA-ASMFETTIRVVGGLLSAYDL-----------SGDK--IFL 217

Query: 256 EIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAH--AAPGGSSSTSEVSTLQLEFNYLST 315
           E A ++ADRLL A+  + + IP   + LK  +AH     GG S  ++  T QLEF  LS 
Sbjct: 218 EKAMDIADRLLPAW-DTQSGIPYNIINLKHGNAHNPTWAGGDSILADSGTEQLEFIALSQ 277

Query: 316 VSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKV 375
            +GDPKY     KV+  +      +GL+PIYI+P T   S  TI  G+ GDS+YEYLLKV
Sbjct: 278 RTGDPKYQQKVEKVISVLNKNFPADGLLPIYINPDTANPSQSTITFGAMGDSFYEYLLKV 337

Query: 376 WLQKRTSGNNNFTYLH--DMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMD 435
           W+     GN      H  DM+E++M G+   LV+KS P    ++ E    S +++  KMD
Sbjct: 338 WV----FGNKTSAVKHYRDMWEKSMNGLLS-LVKKSTPLSFTYICE---KSGNSLIDKMD 397

Query: 436 HLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAP 495
            L CF  G LALGA+     + A  K          L LAE+LA TC+  Y+ T T LA 
Sbjct: 398 ELACFAPGMLALGASG--YSDPAEGKKF--------LTLAEELAWTCYNFYQSTPTKLAG 457

Query: 496 EIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYR 555
           E  +F++      G                      N++RPETVESLF L+R+TG+  Y+
Sbjct: 458 ENYFFNSGSDMSVGTS-------------------WNILRPETVESLFYLWRLTGNKTYQ 517

Query: 556 AWGWEIFEAFERHTKVSSGGYTSLDDV-TAVPPRRRDKMETFFLGETLKYLYLLFGDSST 615
            WGW IFEAFE+++++ S GY  L DV T V   + +KM++FFL ETLKYLYLLF  ++ 
Sbjct: 518 EWGWNIFEAFEKNSRIES-GYVGLKDVNTGV---KDNKMQSFFLAETLKYLYLLFSPTTV 538

Query: 616 IPLDKYVFNTEAHPLPME 629
           IPLD++VFNTEAHPL ++
Sbjct: 578 IPLDEWVFNTEAHPLKIK 538

BLAST of Chy2G024160 vs. TAIR 10
Match: AT1G51590.2 (alpha-mannosidase 1 )

HSP 1 Score: 340.1 bits (871), Expect = 3.8e-93
Identity = 212/485 (43.71%), Postives = 288/485 (59.38%), Query Frame = 0

Query: 145 IHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVSEAGSWIEK 204
           IHAWS Y++YA G DEL P ++ GTD  GGLGAT+VD+LDT  IMG  +   +A  W+  
Sbjct: 2   IHAWSSYEKYAWGKDELQPRTKDGTDSFGGLGATMVDSLDTLYIMGLDEQFQKAREWVAS 61

Query: 205 NLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYGKTFGNEGPKPAVYLEIAKNLADR 264
           +L     +    ++FETTIRV+GGLLSAY L            G K  ++LE AK++ADR
Sbjct: 62  SL--DFDKDYDASMFETTIRVVGGLLSAYDL-----------SGDK--MFLEKAKDIADR 121

Query: 265 LLSAFTSSPTAIPLCDVVLKDSSAHA---APGGSSSTSEVSTLQLEFNYLSTVSGDPKYS 324
           LL A+ ++PT IP   + L++ +AH    A GG S  ++  T QLEF  LS  +GDPKY 
Sbjct: 122 LLPAW-NTPTGIPYNIINLRNGNAHNPSWAAGGDSILADSGTEQLEFIALSQRTGDPKYQ 181

Query: 325 IAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLLKVWLQ-KRTS 384
               KV+  +      +GL+PIYI+P     S  T   G+ GDS+YEYLLKVW+Q  +TS
Sbjct: 182 QKVEKVITELNKNFPADGLLPIYINPDNANPSYSTTTFGAMGDSFYEYLLKVWVQGNKTS 241

Query: 385 GNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMDHLVCFLSGT 444
               +    DM+E++MKG+   LV+KS P+   ++ E    + + +  KMD L CF  G 
Sbjct: 242 AVKPY---RDMWEKSMKGLLS-LVKKSTPSSFTYICE---KNGNNLIDKMDELACFAPGM 301

Query: 445 LALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAPEIAYFHTEE 504
           LALGA+ G   +E          + + L+LA +LA TC+  Y+ T T LA E  YF T  
Sbjct: 302 LALGAS-GYGPDE----------EKKFLSLAGELAWTCYNFYQSTPTKLAGE-NYFFT-- 361

Query: 505 YSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYRAWGWEIFEA 564
            + Q +  G                  N++RPETVESLF L+R+TG+  Y+ WGW IF+A
Sbjct: 362 -AGQDMSVGTS---------------WNILRPETVESLFYLWRLTGNKTYQEWGWNIFQA 421

Query: 565 FERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKYLYLLFGDSSTIPLDKYVFNT 624
           FE++++V S GY  L DV      + +KM++FFL ETLKYLYLLF  SS I LD++VFNT
Sbjct: 422 FEKNSRVES-GYVGLKDVNT--GAKDNKMQSFFLAETLKYLYLLFSPSSVISLDEWVFNT 430

Query: 625 EAHPL 626
           EAHPL
Sbjct: 482 EAHPL 430

BLAST of Chy2G024160 vs. TAIR 10
Match: AT5G43710.1 (Glycosyl hydrolase family 47 protein )

HSP 1 Score: 182.6 bits (462), Expect = 1.0e-45
Identity = 156/508 (30.71%), Postives = 235/508 (46.26%), Query Frame = 0

Query: 137 QQKVKDAFIHAWSGYKRYAMGYDELMPLSQHGTDGLGGLGATVVDALDTAMIMGASDIVS 196
           + +V+  F HA+ GY   A   DEL PLS  G D LGG   T++D+LDT  ++G  +  +
Sbjct: 41  RDEVRGMFYHAFDGYMNNAFPLDELRPLSCQGEDTLGGYALTLIDSLDTLALLGDRERFT 100

Query: 197 EAGSWIEKNLMDRIKEKGQVNLFETTIRVLGGLLSAYHLRAGEYG-----KTFGNEGPKP 256
            +  WI KNL   I +   V++FETTIRVLGGLLSA HL A +Y       ++ NE    
Sbjct: 101 SSVEWIGKNLQFNINK--TVSVFETTIRVLGGLLSA-HLIASDYATGMRIPSYNNE---- 160

Query: 257 AVYLEIAKNLADRLLSAFTSSPTAIPLCDVVLKDSSAHAAPGGSSSTSEVSTLQLEFNYL 316
              L +A+NLA R+L AF  +PT IP   V L            +ST+   TL LEF  L
Sbjct: 161 --LLVLAENLARRMLPAF-DTPTGIPFGSVNLM-YGVDKHESKITSTAGGGTLSLEFGVL 220

Query: 317 STVSGDPKYSIAAMKVLEHMKSLPKVEGLVPIYISPHTGEFSGETIRLGSRGDSYYEYLL 376
           S ++ DP +   A   +  + +      LV  +I+  TGE++ +   +G+  DS+YEYLL
Sbjct: 221 SRLTNDPVFEQVAKNAVRGLWARRSNLDLVGAHINVFTGEWTQKDAGIGTSIDSFYEYLL 280

Query: 377 KVWLQKRTSGNNNFTYLHDMYEEAMKGVKHLLVRKSIPNGLVFVGELPYGSDSTISPKMD 436
           K ++     G+  + Y+   ++EA +     L +        +  E+   S + + P  +
Sbjct: 281 KAYI---LFGDEEYLYI---FQEAYRSAMQYLHKDP------WYVEVNMDSAAIVWPVFN 340

Query: 437 HLVCFLSGTLALGATKGITKEEAMSKSLLNFDDLENLNLAEDLAKTCFEMYEVTSTGLAP 496
            L  F  G   L                    D++          + ++ Y  T  G   
Sbjct: 341 SLQAFWPGLQVLAG------------------DVDPAIRTHTAFFSVWKRYGFTPEG--- 400

Query: 497 EIAYFHTEEYSEQGLDGGNKNSKYIDDIIIKPLDRHNLMRPETVESLFVLYRITGDPKYR 556
               F+    S Q    G K+                 +RPE +ES + LY+ T DP+Y 
Sbjct: 401 ----FNLATLSVQ---YGQKSYP---------------LRPELIESTYWLYKATRDPRYL 460

Query: 557 AWGWEIFEAFERHTKVSSGGYTSLDDVTAVPPRRRDKMETFFLGETLKYLYLLFG---DS 616
             G +   + +   K    GY  + DV     ++ D ME+FFL ET+KYL+LLF    DS
Sbjct: 461 DAGRDFVASLQYGAKCPC-GYCHITDVEL--HKQEDHMESFFLAETVKYLWLLFDLAVDS 479

Query: 617 STIPLD---KYVFNTEAHPLPMEGNIQV 634
             +  +   KY+F+TE H LP+   I +
Sbjct: 521 DNLVDNGPYKYIFSTEGHLLPITPQISL 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q93Y372.5e-26771.92Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 OS=Arabidopsis thaliana OX=3... [more]
Q8J0Q07.9e-11247.35Mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Candida albicans OX=5476 GN=MN... [more]
Q9UKM71.5e-11044.74Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Homo sap... [more]
B2GUY01.4e-10841.74Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Rattus n... [more]
A2AJ151.5e-10741.39Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase OS=Mus musc... [more]
Match NameE-valueIdentityDescription
A0A0A0KLY90.0e+0098.26alpha-1,2-Mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G523340 PE=3 SV=1[more]
A0A1S3AZ690.0e+0097.48alpha-1,2-Mannosidase OS=Cucumis melo OX=3656 GN=LOC103484372 PE=3 SV=1[more]
A0A5A7UEF90.0e+0097.53alpha-1,2-Mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25... [more]
A0A6J1KW660.0e+0090.68alpha-1,2-Mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111496964 PE=3 SV=1[more]
A0A6J1ELX60.0e+0090.52alpha-1,2-Mannosidase OS=Cucurbita moschata OX=3662 GN=LOC111433756 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004134582.10.098.26mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis sativus] >KGN49392.... [more]
XP_008439639.10.097.48PREDICTED: mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis melo][more]
XP_038882608.10.094.64mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X1 [Benincasa hispid... [more]
KAA0052546.10.097.53mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 [Cucumis melo var. makuwa] >... [more]
XP_038882609.10.094.57mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS3 isoform X2 [Benincasa hispid... [more]
Match NameE-valueIdentityDescription
AT1G30000.11.8e-26871.92alpha-mannosidase 3 [more]
AT1G51590.17.4e-9744.13alpha-mannosidase 1 [more]
AT3G21160.16.9e-9543.17alpha-mannosidase 2 [more]
AT1G51590.23.8e-9343.71alpha-mannosidase 1 [more]
AT5G43710.11.0e-4530.71Glycosyl hydrolase family 47 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001382Glycoside hydrolase family 47PRINTSPR00747GLYHDRLASE47coord: 215..233
score: 63.66
coord: 360..377
score: 58.2
coord: 143..163
score: 50.57
coord: 176..190
score: 62.22
coord: 268..287
score: 24.52
coord: 429..445
score: 52.66
coord: 529..553
score: 50.29
coord: 591..611
score: 58.28
IPR001382Glycoside hydrolase family 47PFAMPF01532Glyco_hydro_47coord: 143..626
e-value: 1.5E-157
score: 525.2
IPR012341Six-hairpin glycosidase-like superfamilyGENE3D1.50.10.10coord: 134..633
e-value: 2.3E-172
score: 576.1
NoneNo IPR availablePANTHERPTHR11742MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE-RELATEDcoord: 13..628
NoneNo IPR availablePANTHERPTHR11742:SF88ALPHA-1,2-MANNOSIDASEcoord: 13..628
IPR036026Seven-hairpin glycosidasesSUPERFAMILY48225Seven-hairpin glycosidasescoord: 133..629

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy2G024160.1Chy2G024160.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006491 N-glycan processing
biological_process GO:0030433 ubiquitin-dependent ERAD pathway
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0004571 mannosyl-oligosaccharide 1,2-alpha-mannosidase activity