Chy1G020850 (gene) Cucumber (hystrix) v1

BLAST of Chy1G020850 vs. ExPASy Swiss-Prot
Match: Q9M817 (Protein NRT1/ PTR FAMILY 1.2 OS=Arabidopsis thaliana OX=3702 GN=NPF1.2 PE=1 SV=1)

HSP 1 Score: 708.8 bits (1828), Expect = 1.0e-202
Identity = 355/569 (62.39%), Postives = 448/569 (78.73%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE+ EKV SYGL+PNMI+YL++DY  G AKG N+LF WSAA NF PLLGAFL+DSYLGRF
Sbjct: 34   NEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLGAFLSDSYLGRF 93

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPET-CRSPTAAQMALLAVALTLMSI 709
            LTI   S+++FLGM+LLWLTAM+P  KP  CD     + C S TA+Q+ALL  A  L+SI
Sbjct: 94   LTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLALLYSAFALISI 153

Query: 710  GAGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW 769
            G+GG+RPC+LAFGADQ+D ++NP N+R+LE FFGWYYAS++ +VLIA TGIVYIQ+H+GW
Sbjct: 154  GSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGW 213

Query: 770  KVGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPAST 829
            K+GFGVPA LML A +LF  AS +YV +  TKSLF+  AQ +VAA+K RK  LP    S 
Sbjct: 214  KIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSF 273

Query: 830  KWFYH-KDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIK 889
              +YH KDS    PS KLRFLNKAC++ N E++I  DG A +PW LCT ++VEELK LIK
Sbjct: 274  DCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTTDKVEELKALIK 333

Query: 890  VIPIWSTGVMMSINISQSSFPLLQAKSMDRHIS---STFQIPAGSFGTFVIITIVIWVIL 949
            VIPIWSTG+MMSIN SQSSF LLQA SMDR +S   S+FQ+PAGSFG F II + +WVIL
Sbjct: 334  VIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVIL 393

Query: 950  YDRAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLD 1009
            YDRA++PLASKIRG+P    VK RMG GL  S ++MA+SA+VE+ RR+KAI+QG  ++ +
Sbjct: 394  YDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKKAISQGYANNSN 453

Query: 1010 AVVDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLAS 1069
            AVVD+SA+WL+PQ+ L+GLAEAL AIGQTEF+Y+EFPK+MSS+A+SLFGLGMAVA+LLAS
Sbjct: 454  AVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLAS 513

Query: 1070 AIMSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQR 1129
             +++ V+ +TS+ GKESWVS NINKGH+  YYW+LAI+S INV+YYV+CSW+YGP VDQ 
Sbjct: 514  VVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVICSWSYGPLVDQV 573

Query: 1130 RTAMDDGKISSNEDELSMLDARVKEEEGE 1154
            R    +G+++   +E  ++D   K  E E
Sbjct: 574  R----NGRVNGVREEEELIDIVGKGFEKE 598

BLAST of Chy1G020850 vs. ExPASy Swiss-Prot
Match: Q8LPL2 (Protein NRT1/ PTR FAMILY 1.1 OS=Arabidopsis thaliana OX=3702 GN=NPF1.1 PE=1 SV=2)

HSP 1 Score: 681.8 bits (1758), Expect = 1.4e-194
Identity = 340/556 (61.15%), Postives = 426/556 (76.62%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE  EKV SYGL+ NMILYLM DY LG  KG  +LF W AA NFMPL+GAFL+DSYLGRF
Sbjct: 36   NEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVGAFLSDSYLGRF 95

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
            LTI   S+++ LGM++LWLTAM+P  KP  C       C S T++Q+ALL  A  L+SIG
Sbjct: 96   LTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLALLYTAFALISIG 155

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            +GG+RPC+LAFGADQ+D ++NP N+R+LE FFGWYYAS+S +VLIA T IVYIQDH+GWK
Sbjct: 156  SGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWK 215

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            +GFG+PA LML A  LF  AS +YVK+  +KSLF+  AQVV AA+  R   LP    S  
Sbjct: 216  IGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRD 275

Query: 830  WFYH-KDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKV 889
             +Y  KDS    PSDKLRFLNKAC + N ++D+  DG A + W LCT +QVE+LK L+KV
Sbjct: 276  CYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKV 335

Query: 890  IPIWSTGVMMSINISQSSFPLLQAKSMDRHIS--STFQIPAGSFGTFVIITIVIWVILYD 949
            IP+WSTG+MMSIN+SQ+SF LLQAKSMDR +S  STFQIPAGSFG F II ++ WV+LYD
Sbjct: 336  IPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYD 395

Query: 950  RAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAV 1009
            RAILPLASKIRG+PV   VK RMG GL  S ++MA+SA VE+ RR+ AI+QG+ +D ++ 
Sbjct: 396  RAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAISQGLANDANST 455

Query: 1010 VDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAI 1069
            V +SA+WL+PQ+ L+GLAEAL  IGQTEF+Y+EFPK+MSS+A+SLFGLGMAVAN+LAS I
Sbjct: 456  VSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLGMAVANILASVI 515

Query: 1070 MSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRT 1129
            ++ V N +SK G  SW+  NINKGH++ YYW+LAILS +NV+YYVVCSW+YGP+VDQ R 
Sbjct: 516  LNAVKN-SSKQGNVSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSWSYGPTVDQVRN 575

Query: 1130 AMDDGKISSNEDELSM 1143
               +G     E+ + +
Sbjct: 576  DKVNGMRKEEEEVIKL 590

BLAST of Chy1G020850 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 5.2e-109
Identity = 217/559 (38.82%), Postives = 334/559 (59.75%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE+LE++GS GL+ N ++YL K ++L      N++  WS   N  PL+GA+++D+Y+GRF
Sbjct: 64   NETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVGAYISDTYVGRF 123

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
             TI F S AT LG++ + LTA  P   P  C+   P +C  P   Q+ +L + L  +S+G
Sbjct: 124  KTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVG 183

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            +GG+RPC++ FG DQ D+R     K +   FF WYY + +  ++I  T +VYIQD V W 
Sbjct: 184  SGGIRPCSIPFGVDQFDQRTEEGVKGVAS-FFNWYYMTFTVVLIITQTVVVYIQDQVSWI 243

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            +GF +P GLM  A V+FFA    YV  K   S+FS  AQV+VAA K RK  LP     T 
Sbjct: 244  IGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDDGTV 303

Query: 830  WFYH---KDSLFTQ--PSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKT 889
             +Y    K S+ ++   S++ R L+KA VV   E D+  +G  AD W LC+V++VEE+K 
Sbjct: 304  TYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWRLCSVQEVEEVKC 363

Query: 890  LIKVIPIWSTGVM-MSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVI 949
            LI+++PIWS G++ ++   +Q +F + QA  MDR++   F+IPAGS     ++TI I++ 
Sbjct: 364  LIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTIGIFLP 423

Query: 950  LYDRAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDL 1009
             YDR  +P   +I G      +  R+G G++ +  SM ++ IVE +RR ++I  G   D 
Sbjct: 424  FYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAG---DP 483

Query: 1010 DAVVDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLA 1069
              +  MS  WL PQ  L GL EA N IGQ EF+ S+FP+ M S+A+SLF L  A ++ L+
Sbjct: 484  TGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLS 543

Query: 1070 SAIMSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQ 1129
            S +++ V   +    +  W++KN+N G  + +Y+L+A+L V+N++Y+  C+  Y   V  
Sbjct: 544  SFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFWYCARGYRYKVG- 603

Query: 1130 RRTAMDDGKISSNEDELSM 1143
                ++D +   + D++ M
Sbjct: 604  --LPIEDFEEDKSSDDVEM 613

BLAST of Chy1G020850 vs. ExPASy Swiss-Prot
Match: Q944G5 (Protein NRT1/ PTR FAMILY 2.10 OS=Arabidopsis thaliana OX=3702 GN=NPF2.10 PE=1 SV=3)

HSP 1 Score: 369.8 bits (948), Expect = 1.2e-100
Identity = 210/562 (37.37%), Postives = 332/562 (59.07%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE+ EK+G  G + N+++YL   +NL       I+  +S  INF   + AFL D+Y GR+
Sbjct: 74   NETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFGTFIAAFLCDTYFGRY 133

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
             T+    IA FLG  ++ LTA +PS  P  C   +  +C  P+  Q+  L + L  + +G
Sbjct: 134  KTLSVAVIACFLGSFVILLTAAIPSLHPVACG--NKISCEGPSVGQILFLLMGLGFLVVG 193

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            AGG+RPC LAFGADQ + + + + K+ +  FF WY+ + +F+ +I+LT +VYIQ +V W 
Sbjct: 194  AGGIRPCNLAFGADQFNPK-SESGKKGINSFFNWYFFTFTFAQIISLTAVVYIQSNVSWT 253

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            +G  +P  LM  A V+FFA   +YVK KA+ S  +  A+V+ AA K R    P       
Sbjct: 254  IGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLK-PVKQPWVN 313

Query: 830  WFYHKDSLFTQP----SDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTL 889
             + H  S +       +D+ RFL+KA ++  PE+ +  DGTA+DPW LCT++QVEE+K +
Sbjct: 314  LYNHIPSNYANTTLKYTDQFRFLDKAAIM-TPEEKLNSDGTASDPWKLCTLQQVEEVKCI 373

Query: 890  IKVIPIW--STGVMMSINISQSSFPLLQAKSMDRHI-SSTFQIPAGSFGTFVIITIVIWV 949
            ++VIPIW  ST   ++I I Q ++P+ QA   DR + S  F+IPA ++  F++  + +++
Sbjct: 374  VRVIPIWFASTIYYLAITI-QMTYPVFQALQSDRRLGSGGFRIPAATYVVFLMTGMTVFI 433

Query: 950  ILYDRAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQ---GI 1009
            I YDR ++P   ++ G      +  R+GAG   + MS+ +S  +E  RR  A+ +   G+
Sbjct: 434  IFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSGFIEERRRNFALTKPTLGM 493

Query: 1010 VDDLDAVVDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVA 1069
                  +  MSALWLIPQ  L G+AEA  AIGQ EFYY +FP+ M S A S+F +G  V+
Sbjct: 494  APRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFPENMKSFAGSIFYVGAGVS 553

Query: 1070 NLLASAIMSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCS--WAY 1129
            + LAS ++STV   T+     +W+++++NK   + +Y++L  L V+N+ Y+++ +  + Y
Sbjct: 554  SYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLTGLMVVNMAYFLLMARWYRY 613

Query: 1130 GPSVDQRRTAMDDGKISSNEDE 1140
                D+     D  +I +NE+E
Sbjct: 614  KGGNDE-----DITEIETNEEE 624

BLAST of Chy1G020850 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 365.9 bits (938), Expect = 1.7e-99
Identity = 196/534 (36.70%), Postives = 316/534 (59.18%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE+LEK+GS G+  N +LYL   +++   +  N+ + W    NF PLLGA ++D+Y+GRF
Sbjct: 27   NETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLGALISDAYIGRF 86

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
             TI + S+ + LG++ + LTA +P   PP C+  HP+ C  P   Q+ +L + L  +SIG
Sbjct: 87   KTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGILFLGLGFLSIG 146

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            +GG+RPC++ FG DQ D+R     K +   FF WYY + +  ++ + T +VY+Q  V W 
Sbjct: 147  SGGIRPCSIPFGVDQFDQRTEQGLKGVAS-FFNWYYLTLTMVLIFSHTVVVYLQT-VSWV 206

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            +GF +P  LM  A VLFF     YV  K   S+FS  A+V+VAA K R   +      T+
Sbjct: 207  IGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDLKISLVDDGTE 266

Query: 830  WFYH---KDSLFTQ--PSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKT 889
             +Y    K  + ++   +D+ +FL+KA V+   + D+  +G  A+ W LC++++VEE+K 
Sbjct: 267  EYYEPPVKPGVLSKLPLTDQFKFLDKAAVIL--DGDLTSEGVPANKWRLCSIQEVEEVKC 326

Query: 890  LIKVIPIWSTGVMMSINI-SQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVI 949
            LI+V+P+WS G++  + + +Q++F + QA  MDRH+   F+IPA S      ITI IWV 
Sbjct: 327  LIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVISYITIGIWVP 386

Query: 950  LYDRAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDL 1009
            +Y+  ++P   ++R       +  RMG G++ + +SM  +  VE +RR +A         
Sbjct: 387  IYEHLLVPFLWRMR--KFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTRA--------- 446

Query: 1010 DAVVDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLA 1069
              +  MS  WL     L GL E+ N IG  EF+ S+FP+ M S+A+SLF L  A AN L+
Sbjct: 447  TEMTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAAANYLS 506

Query: 1070 SAIMSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAY 1118
            S +++TV  V+       W++K++++G  + +Y+L+A+L V+N++Y+  C+  Y
Sbjct: 507  SLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLVYFWYCAHRY 545

BLAST of Chy1G020850 vs. ExPASy TrEMBL
Match: A0A0A0KAH5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G447020 PE=3 SV=1)

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 572/574 (99.65%), Postives = 573/574 (99.83%), Query Frame = 0

Query: 589  LNESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGR 648
            +NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGR
Sbjct: 50   VNESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGR 109

Query: 649  FLTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSI 708
            FLTIGFGSIATFLGMLLLWLTAMVPSTKPP CDQLHPETCRSPTAAQMALLAVALTLMSI
Sbjct: 110  FLTIGFGSIATFLGMLLLWLTAMVPSTKPPACDQLHPETCRSPTAAQMALLAVALTLMSI 169

Query: 709  GAGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW 768
            GAGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW
Sbjct: 170  GAGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW 229

Query: 769  KVGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPAST 828
            KVGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPAST
Sbjct: 230  KVGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPAST 289

Query: 829  KWFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKV 888
            KWFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKV
Sbjct: 290  KWFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKV 349

Query: 889  IPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRA 948
            IPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRA
Sbjct: 350  IPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRA 409

Query: 949  ILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVD 1008
            ILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVD
Sbjct: 410  ILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVD 469

Query: 1009 MSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMS 1068
            MSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMS
Sbjct: 470  MSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMS 529

Query: 1069 TVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAM 1128
            TVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAM
Sbjct: 530  TVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAM 589

Query: 1129 DDGKISSNEDELSMLDARVKEEEGELHKVKELEA 1163
            DDGKISSNEDELSMLDARVKEEEGELHKVKELEA
Sbjct: 590  DDGKISSNEDELSMLDARVKEEEGELHKVKELEA 623

BLAST of Chy1G020850 vs. ExPASy TrEMBL
Match: A0A6J1KDE4 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita maxima OX=3661 GN=LOC111494604 PE=3 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 6.4e-296
Identity = 520/573 (90.75%), Postives = 546/573 (95.29%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NES EKV SYGL+PNMILYLMKDYN+GFA+GNNILFFWSAAINFMPLLGAFL+DSYLGRF
Sbjct: 38   NESFEKVASYGLLPNMILYLMKDYNMGFAEGNNILFFWSAAINFMPLLGAFLSDSYLGRF 97

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
            LTIG GSIA+FLGMLLLWLTAM+P+TKPPVCDQLHPETCRSPTAAQ+ LLA + TLMSIG
Sbjct: 98   LTIGLGSIASFLGMLLLWLTAMIPATKPPVCDQLHPETCRSPTAAQLTLLAASFTLMSIG 157

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            AGG+RPCTLAFGADQIDRR++PNNKR+LERFFGWYYASASFSVLIALTGIVYIQDHVGWK
Sbjct: 158  AGGIRPCTLAFGADQIDRRNDPNNKRILERFFGWYYASASFSVLIALTGIVYIQDHVGWK 217

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            VGFGVPA LML ATVLFFAASSIYVKQKATKSLFS  AQV VAAFKNRK  LP++ AS K
Sbjct: 218  VGFGVPASLMLVATVLFFAASSIYVKQKATKSLFSRLAQVAVAAFKNRKIALPSASASNK 277

Query: 830  WFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKVI 889
            WFYH DS FTQPSDKLRFLN+ACVVKNPEQDIA DGTAADPWSLCTVEQVEELKTLIKVI
Sbjct: 278  WFYHTDSCFTQPSDKLRFLNRACVVKNPEQDIAADGTAADPWSLCTVEQVEELKTLIKVI 337

Query: 890  PIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRAI 949
            PIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVI YDRAI
Sbjct: 338  PIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVIFYDRAI 397

Query: 950  LPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVDM 1009
            LPLASKIRGKPVHFGVKSRMGAGL+CSAMSMALSAIVENIRR KAI QGIVDD +AVV+M
Sbjct: 398  LPLASKIRGKPVHFGVKSRMGAGLVCSAMSMALSAIVENIRRGKAIRQGIVDDPNAVVNM 457

Query: 1010 SALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMST 1069
            SA+WL+PQHCLNGLAEALNAI QTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMST
Sbjct: 458  SAMWLVPQHCLNGLAEALNAIAQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMST 517

Query: 1070 VDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAMD 1129
            VDNVTSKGGKESWVSKNIN+GHFEKYYWLL ILSVINVLYYVVCSWAYGPSVDQRRTAMD
Sbjct: 518  VDNVTSKGGKESWVSKNINRGHFEKYYWLLTILSVINVLYYVVCSWAYGPSVDQRRTAMD 577

Query: 1130 DGKISSNEDELSMLDARVKEEEGELHKVKELEA 1163
            DGKISS+E+ELSML+ARVK EEGEL K KEL+A
Sbjct: 578  DGKISSHEEELSMLEARVK-EEGELQKPKELQA 609

BLAST of Chy1G020850 vs. ExPASy TrEMBL
Match: A0A6J1HMR3 (protein NRT1/ PTR FAMILY 1.2-like OS=Cucurbita moschata OX=3662 GN=LOC111464351 PE=3 SV=1)

HSP 1 Score: 1020.8 bits (2638), Expect = 4.6e-294
Identity = 520/575 (90.43%), Postives = 545/575 (94.78%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NES EKV SYGL+PNMILYLMKDYN+GFA+GNNILFFWSAAINFMPLLGAFL+DSYLGRF
Sbjct: 38   NESFEKVASYGLLPNMILYLMKDYNMGFAEGNNILFFWSAAINFMPLLGAFLSDSYLGRF 97

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
            LTIG GSIA+FLGMLLLWLTAM+P+TKP VCDQLHPETCRSPTAAQ+ LLA + TLMSIG
Sbjct: 98   LTIGLGSIASFLGMLLLWLTAMIPATKPTVCDQLHPETCRSPTAAQLTLLAASFTLMSIG 157

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            AGG+RPCTLAFGADQIDRRD+PNNKR+LERFFGWYYASASFSVLIALTGIVYIQDHVGWK
Sbjct: 158  AGGIRPCTLAFGADQIDRRDDPNNKRILERFFGWYYASASFSVLIALTGIVYIQDHVGWK 217

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTS--PAS 829
            VGFGVPA LML ATVLFFAASSIYVKQKATKSLFS  AQV VAAFKNRK  LP++   AS
Sbjct: 218  VGFGVPASLMLVATVLFFAASSIYVKQKATKSLFSRLAQVAVAAFKNRKIALPSASGSAS 277

Query: 830  TKWFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIK 889
             KWFYH DS FTQPSDKLRFLNKACVVKNPEQDIA DGTAADPWSLCTVEQVEELKTLIK
Sbjct: 278  NKWFYHTDSCFTQPSDKLRFLNKACVVKNPEQDIAADGTAADPWSLCTVEQVEELKTLIK 337

Query: 890  VIPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDR 949
            VIPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDR
Sbjct: 338  VIPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDR 397

Query: 950  AILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVV 1009
            AILPLASKIRGKPVHFGVKSRMGAGL+CSAMSMALSAIVENIRR KAI QGIVDD +AVV
Sbjct: 398  AILPLASKIRGKPVHFGVKSRMGAGLVCSAMSMALSAIVENIRRGKAIRQGIVDDPNAVV 457

Query: 1010 DMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIM 1069
            +MSA+WL+PQHCLNGLAEALNAI QTEFYYSEFPKTMSSVASSLFG+GMAVANLLASAIM
Sbjct: 458  NMSAMWLVPQHCLNGLAEALNAIAQTEFYYSEFPKTMSSVASSLFGMGMAVANLLASAIM 517

Query: 1070 STVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTA 1129
            STVDNVTSKGGKESWVSKNIN+GHFE YYWLL ILSVINVLYYVVCSWAYGPSVDQRRTA
Sbjct: 518  STVDNVTSKGGKESWVSKNINRGHFENYYWLLTILSVINVLYYVVCSWAYGPSVDQRRTA 577

Query: 1130 MDDGKISSNEDELSMLDARVKEEEGELHKVKELEA 1163
            MDDGKISS+E+ELSML+ARVK EEGEL K KEL+A
Sbjct: 578  MDDGKISSHEEELSMLEARVK-EEGELQKPKELQA 611

BLAST of Chy1G020850 vs. ExPASy TrEMBL
Match: A0A6J1DHM6 (protein NRT1/ PTR FAMILY 1.2-like OS=Momordica charantia OX=3673 GN=LOC111020557 PE=3 SV=1)

HSP 1 Score: 978.4 bits (2528), Expect = 2.6e-281
Identity = 493/577 (85.44%), Postives = 533/577 (92.37%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NES EKV SYGL+PNMILYLMKDYNLGFAKGNN+LFFWSAA NFMPLLGAFLADSYLGRF
Sbjct: 46   NESFEKVASYGLLPNMILYLMKDYNLGFAKGNNVLFFWSAATNFMPLLGAFLADSYLGRF 105

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
            LTIGFGSIA+FLGM LLWLTAMVP+TKPP CD LHP TCRSP+A QM LL  A  LMS+G
Sbjct: 106  LTIGFGSIASFLGMALLWLTAMVPATKPPACDPLHPLTCRSPSAGQMTLLVAAFGLMSVG 165

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            AGGVRPCTLAFGADQIDRR++PNNKR+LERFFGWYYASASFSVLIALTGIVYIQDH+GWK
Sbjct: 166  AGGVRPCTLAFGADQIDRREDPNNKRILERFFGWYYASASFSVLIALTGIVYIQDHLGWK 225

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            +GFGVPA LMLFATVLFFAAS IYVKQ+ATKSLFSSF QV VAAFKNRK PLP  PAS K
Sbjct: 226  IGFGVPASLMLFATVLFFAASPIYVKQRATKSLFSSFVQVAVAAFKNRKLPLP-PPASDK 285

Query: 830  WFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKVI 889
            WFYHKDS  TQPS+KLRFLNKACVVKN E+DI  DG A+DPWSLCTVEQVEELKTLIKVI
Sbjct: 286  WFYHKDSYLTQPSEKLRFLNKACVVKNAEEDILADGRASDPWSLCTVEQVEELKTLIKVI 345

Query: 890  PIWSTGVMMSINISQSSFPLLQAKSMDRHIS--STFQIPAGSFGTFVIITIVIWVILYDR 949
            PIWSTGVMMSIN+SQSSFPLLQAKSMDR IS  S+FQIPAGSFGTFVIITIV+WVILYDR
Sbjct: 346  PIWSTGVMMSINVSQSSFPLLQAKSMDRRISSASSFQIPAGSFGTFVIITIVLWVILYDR 405

Query: 950  AILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVV 1009
            AILPLASKIRGKPVHFGVKSRMGAGL+CS ++MALSA+VEN+RRRKAI QG++DD +AVV
Sbjct: 406  AILPLASKIRGKPVHFGVKSRMGAGLLCSFLAMALSAVVENVRRRKAIQQGLLDDPNAVV 465

Query: 1010 DMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIM 1069
            D+SALWL+PQHCL+GLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAI+
Sbjct: 466  DISALWLVPQHCLSGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIL 525

Query: 1070 STVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTA 1129
            S VD VTSKGGK+SWVSKNIN+ HFE YYW+LAILS +N+LY+VVCSWAYGP VDQRRTA
Sbjct: 526  SAVDEVTSKGGKQSWVSKNINRAHFENYYWILAILSGVNILYFVVCSWAYGPCVDQRRTA 585

Query: 1130 MDDGKISSNEDELSMLDARVKEEEG---ELHKVKELE 1162
            MDDGKISSNE+ELSML+ARVKEEEG   EL + KEL+
Sbjct: 586  MDDGKISSNEEELSMLEARVKEEEGKMVELQRPKELQ 621

BLAST of Chy1G020850 vs. ExPASy TrEMBL
Match: A0A5D3C0N9 (Protein NRT1/ PTR FAMILY 1.2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G002140 PE=3 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 1.1e-276
Identity = 489/500 (97.80%), Postives = 495/500 (99.00%), Query Frame = 0

Query: 663  MLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIGAGGVRPCTLAFGA 722
            MLLLWLTAMVPSTKPP CDQLHPETC+SPTAAQMA LAVAL+LMSIGAGGVRPCTLAFGA
Sbjct: 1    MLLLWLTAMVPSTKPPACDQLHPETCQSPTAAQMAFLAVALSLMSIGAGGVRPCTLAFGA 60

Query: 723  DQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWKVGFGVPAGLMLFA 782
            DQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWKVGFGVPAGLMLFA
Sbjct: 61   DQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWKVGFGVPAGLMLFA 120

Query: 783  TVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTKWFYHKDSLFTQPS 842
            TVLFFAASSIYVKQKATKSLFSSFAQV VAAFKNRKFPLP SPASTKWFYHKDS+FTQPS
Sbjct: 121  TVLFFAASSIYVKQKATKSLFSSFAQVAVAAFKNRKFPLPASPASTKWFYHKDSIFTQPS 180

Query: 843  DKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKVIPIWSTGVMMSINI 902
            DKLRFLNKACVVKNPEQDIAGDGTAA+PWSLCTVEQVEELKTLIKVIPIWSTGVMMSIN+
Sbjct: 181  DKLRFLNKACVVKNPEQDIAGDGTAANPWSLCTVEQVEELKTLIKVIPIWSTGVMMSINV 240

Query: 903  SQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRAILPLASKIRGKPVH 962
            SQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRAILPLASKIRGKPVH
Sbjct: 241  SQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRAILPLASKIRGKPVH 300

Query: 963  FGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVDMSALWLIPQHCLNG 1022
            FGVKSRMGAGLICSAMSMALSAIVEN+RRRKAIAQGIVDDLDAVVDMSALWLIPQHCLNG
Sbjct: 301  FGVKSRMGAGLICSAMSMALSAIVENVRRRKAIAQGIVDDLDAVVDMSALWLIPQHCLNG 360

Query: 1023 LAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMSTVDNVTSKGGKESW 1082
            LAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMSTVDNVTSKGGKESW
Sbjct: 361  LAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMSTVDNVTSKGGKESW 420

Query: 1083 VSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAMDDGKISSNEDELSM 1142
            VSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAMDDGKISSNEDELSM
Sbjct: 421  VSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAMDDGKISSNEDELSM 480

Query: 1143 LDARVKEEEGELHKVKELEA 1163
            LDARVKEEEGELHKVK LEA
Sbjct: 481  LDARVKEEEGELHKVKGLEA 500

BLAST of Chy1G020850 vs. NCBI nr
Match: XP_011659573.1 (protein NRT1/ PTR FAMILY 1.2 [Cucumis sativus] >KGN45387.1 hypothetical protein Csa_016404 [Cucumis sativus])

HSP 1 Score: 1117 bits (2890), Expect = 0.0
Identity = 572/574 (99.65%), Postives = 573/574 (99.83%), Query Frame = 0

Query: 589  LNESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGR 648
            +NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGR
Sbjct: 50   VNESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGR 109

Query: 649  FLTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSI 708
            FLTIGFGSIATFLGMLLLWLTAMVPSTKPP CDQLHPETCRSPTAAQMALLAVALTLMSI
Sbjct: 110  FLTIGFGSIATFLGMLLLWLTAMVPSTKPPACDQLHPETCRSPTAAQMALLAVALTLMSI 169

Query: 709  GAGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW 768
            GAGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW
Sbjct: 170  GAGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW 229

Query: 769  KVGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPAST 828
            KVGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPAST
Sbjct: 230  KVGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPAST 289

Query: 829  KWFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKV 888
            KWFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKV
Sbjct: 290  KWFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKV 349

Query: 889  IPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRA 948
            IPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRA
Sbjct: 350  IPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRA 409

Query: 949  ILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVD 1008
            ILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVD
Sbjct: 410  ILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVD 469

Query: 1009 MSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMS 1068
            MSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMS
Sbjct: 470  MSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMS 529

Query: 1069 TVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAM 1128
            TVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAM
Sbjct: 530  TVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAM 589

Query: 1129 DDGKISSNEDELSMLDARVKEEEGELHKVKELEA 1162
            DDGKISSNEDELSMLDARVKEEEGELHKVKELEA
Sbjct: 590  DDGKISSNEDELSMLDARVKEEEGELHKVKELEA 623

BLAST of Chy1G020850 vs. NCBI nr
Match: XP_038897596.1 (protein NRT1/ PTR FAMILY 1.2 [Benincasa hispida])

HSP 1 Score: 1062 bits (2746), Expect = 0.0
Identity = 539/574 (93.90%), Postives = 553/574 (96.34%), Query Frame = 0

Query: 589  LNESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGR 648
            +NESLEKV SYGL+PNMILYLMKDYNLGFAKGNNILFFWSAA NFMPLLGAFL+DSYLGR
Sbjct: 39   VNESLEKVASYGLLPNMILYLMKDYNLGFAKGNNILFFWSAATNFMPLLGAFLSDSYLGR 98

Query: 649  FLTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSI 708
            FLTIGFGSIA+FLGMLLLWLTAMVP+TKPP CDQLHPETCRSPTA QM LLA AL LMSI
Sbjct: 99   FLTIGFGSIASFLGMLLLWLTAMVPATKPPACDQLHPETCRSPTAGQMTLLATALILMSI 158

Query: 709  GAGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW 768
            GAGGVRPCTLAFGADQIDRR+NPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW
Sbjct: 159  GAGGVRPCTLAFGADQIDRRENPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW 218

Query: 769  KVGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPAST 828
            KVGFGVPA LMLF TVLFFAASSIYVKQKATKSLFSSFAQV VAAFKNR+FPLP SPAS 
Sbjct: 219  KVGFGVPASLMLFGTVLFFAASSIYVKQKATKSLFSSFAQVAVAAFKNRRFPLPPSPASN 278

Query: 829  KWFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKV 888
            KWFYHKDS FTQPSDKLRFLNKACVVKNP QDIAGDGTAADPWSLCTVEQVEELKTLIKV
Sbjct: 279  KWFYHKDSCFTQPSDKLRFLNKACVVKNPGQDIAGDGTAADPWSLCTVEQVEELKTLIKV 338

Query: 889  IPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRA 948
            IPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRA
Sbjct: 339  IPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRA 398

Query: 949  ILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVD 1008
            ILPLASKIRGKPVHFGVKSRMGAGL+CSAMSMALSAIVENIRRRKAIA GIVDD +AVVD
Sbjct: 399  ILPLASKIRGKPVHFGVKSRMGAGLVCSAMSMALSAIVENIRRRKAIALGIVDDPNAVVD 458

Query: 1009 MSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMS 1068
            MSALWL+PQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMS
Sbjct: 459  MSALWLVPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMS 518

Query: 1069 TVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAM 1128
            TVDNVTSKGGKESWVSKNINKGHFE YYWLLAILS IN+LYYVVCSWAYGPSVDQRRTAM
Sbjct: 519  TVDNVTSKGGKESWVSKNINKGHFENYYWLLAILSAINILYYVVCSWAYGPSVDQRRTAM 578

Query: 1129 DDGKISSNEDELSMLDARVKEEEGELHKVKELEA 1162
            DDGKISSNE+ELSML+ARVKEEEGE  K KEL+A
Sbjct: 579  DDGKISSNEEELSMLEARVKEEEGEFQKAKELQA 612

BLAST of Chy1G020850 vs. NCBI nr
Match: XP_023515294.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1025 bits (2650), Expect = 0.0
Identity = 521/573 (90.92%), Postives = 546/573 (95.29%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NES EKV SYGL+PNMI+YLMKDYN+GFA+GNNILFFWSAAINFMPLLGAFL+DSYLGRF
Sbjct: 38   NESFEKVASYGLLPNMIVYLMKDYNMGFAEGNNILFFWSAAINFMPLLGAFLSDSYLGRF 97

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
            LTIG GSIA+FLGMLLLWLTAM+P+TKPPVCDQLHPETCRSPTAAQ+ LLA + TLMSIG
Sbjct: 98   LTIGLGSIASFLGMLLLWLTAMIPATKPPVCDQLHPETCRSPTAAQLTLLAASFTLMSIG 157

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            AGG+RPCTLAFGADQIDRRD+PNNKR+LERFFGWYYASASFSVLIALTGIVYIQDHVGWK
Sbjct: 158  AGGIRPCTLAFGADQIDRRDDPNNKRILERFFGWYYASASFSVLIALTGIVYIQDHVGWK 217

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            VGFGVPA LML ATVLFFAASSIYVKQKATKSLFS  AQV VAAFKNRK  LP++ AS K
Sbjct: 218  VGFGVPASLMLVATVLFFAASSIYVKQKATKSLFSRLAQVAVAAFKNRKIALPSASASNK 277

Query: 830  WFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKVI 889
            WFYH DS FTQPSDKLRFLNKACVVKNPEQDIA DGTAADPWSLCTVEQVEELKTLIKVI
Sbjct: 278  WFYHTDSCFTQPSDKLRFLNKACVVKNPEQDIAADGTAADPWSLCTVEQVEELKTLIKVI 337

Query: 890  PIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRAI 949
            PIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRAI
Sbjct: 338  PIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRAI 397

Query: 950  LPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVDM 1009
            LPLASKIRGKPVHFGVKSRMGAGL+CSAMSMALSAIVENIRR KAI QGIVDD +AVV+M
Sbjct: 398  LPLASKIRGKPVHFGVKSRMGAGLVCSAMSMALSAIVENIRRGKAIRQGIVDDPNAVVNM 457

Query: 1010 SALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMST 1069
            SA+WL+PQHCLNGLAEALNAI QTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMST
Sbjct: 458  SAMWLVPQHCLNGLAEALNAIAQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMST 517

Query: 1070 VDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAMD 1129
            VDNVTSKGGKESWVSKNIN+GHFE YYWLL ILSVINVLYYVVCSWAYGPSVDQRRTAMD
Sbjct: 518  VDNVTSKGGKESWVSKNINRGHFENYYWLLTILSVINVLYYVVCSWAYGPSVDQRRTAMD 577

Query: 1130 DGKISSNEDELSMLDARVKEEEGELHKVKELEA 1162
            DGKISS+E+ELSML+ARVKEE GEL K KEL+A
Sbjct: 578  DGKISSHEEELSMLEARVKEE-GELQKPKELQA 609

BLAST of Chy1G020850 vs. NCBI nr
Match: XP_023000347.1 (protein NRT1/ PTR FAMILY 1.2-like [Cucurbita maxima])

HSP 1 Score: 1023 bits (2646), Expect = 0.0
Identity = 520/573 (90.75%), Postives = 546/573 (95.29%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NES EKV SYGL+PNMILYLMKDYN+GFA+GNNILFFWSAAINFMPLLGAFL+DSYLGRF
Sbjct: 38   NESFEKVASYGLLPNMILYLMKDYNMGFAEGNNILFFWSAAINFMPLLGAFLSDSYLGRF 97

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
            LTIG GSIA+FLGMLLLWLTAM+P+TKPPVCDQLHPETCRSPTAAQ+ LLA + TLMSIG
Sbjct: 98   LTIGLGSIASFLGMLLLWLTAMIPATKPPVCDQLHPETCRSPTAAQLTLLAASFTLMSIG 157

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            AGG+RPCTLAFGADQIDRR++PNNKR+LERFFGWYYASASFSVLIALTGIVYIQDHVGWK
Sbjct: 158  AGGIRPCTLAFGADQIDRRNDPNNKRILERFFGWYYASASFSVLIALTGIVYIQDHVGWK 217

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            VGFGVPA LML ATVLFFAASSIYVKQKATKSLFS  AQV VAAFKNRK  LP++ AS K
Sbjct: 218  VGFGVPASLMLVATVLFFAASSIYVKQKATKSLFSRLAQVAVAAFKNRKIALPSASASNK 277

Query: 830  WFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKVI 889
            WFYH DS FTQPSDKLRFLN+ACVVKNPEQDIA DGTAADPWSLCTVEQVEELKTLIKVI
Sbjct: 278  WFYHTDSCFTQPSDKLRFLNRACVVKNPEQDIAADGTAADPWSLCTVEQVEELKTLIKVI 337

Query: 890  PIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDRAI 949
            PIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVI YDRAI
Sbjct: 338  PIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVIFYDRAI 397

Query: 950  LPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVVDM 1009
            LPLASKIRGKPVHFGVKSRMGAGL+CSAMSMALSAIVENIRR KAI QGIVDD +AVV+M
Sbjct: 398  LPLASKIRGKPVHFGVKSRMGAGLVCSAMSMALSAIVENIRRGKAIRQGIVDDPNAVVNM 457

Query: 1010 SALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMST 1069
            SA+WL+PQHCLNGLAEALNAI QTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMST
Sbjct: 458  SAMWLVPQHCLNGLAEALNAIAQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIMST 517

Query: 1070 VDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTAMD 1129
            VDNVTSKGGKESWVSKNIN+GHFEKYYWLL ILSVINVLYYVVCSWAYGPSVDQRRTAMD
Sbjct: 518  VDNVTSKGGKESWVSKNINRGHFEKYYWLLTILSVINVLYYVVCSWAYGPSVDQRRTAMD 577

Query: 1130 DGKISSNEDELSMLDARVKEEEGELHKVKELEA 1162
            DGKISS+E+ELSML+ARVKEE GEL K KEL+A
Sbjct: 578  DGKISSHEEELSMLEARVKEE-GELQKPKELQA 609

BLAST of Chy1G020850 vs. NCBI nr
Match: KAG6593470.1 (Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025813.1 Protein NRT1/ PTR FAMILY 1.2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1021 bits (2640), Expect = 0.0
Identity = 522/575 (90.78%), Postives = 546/575 (94.96%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NES EKV SYGL+PNMILYLMKDYN+GFA+GNNILFFWSAAINFMPLLGAFL+DSYLGRF
Sbjct: 38   NESFEKVASYGLLPNMILYLMKDYNMGFAEGNNILFFWSAAINFMPLLGAFLSDSYLGRF 97

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
            LTIG GSIA+FLGMLLLWLTAM+P+TKPPVCDQLHPETCRSPTAAQ+ LLA + TLMSIG
Sbjct: 98   LTIGLGSIASFLGMLLLWLTAMIPATKPPVCDQLHPETCRSPTAAQLTLLAASFTLMSIG 157

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            AGG+RPCTLAFGADQIDRRD+PNNKR+LERFFGWYYASASFSVLIALTGIVYIQDHVGWK
Sbjct: 158  AGGIRPCTLAFGADQIDRRDDPNNKRILERFFGWYYASASFSVLIALTGIVYIQDHVGWK 217

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSP--AS 829
            VGFGVPA LML ATVLFFAASSIYVKQKATKSLFS  AQV VAAFKNRK  LP++   AS
Sbjct: 218  VGFGVPASLMLVATVLFFAASSIYVKQKATKSLFSRLAQVAVAAFKNRKIALPSASGSAS 277

Query: 830  TKWFYHKDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIK 889
             KWFYH DS FTQPSDKLRFLNKACVVKNPEQDIA DGTAADPWSLCTVEQVEELKTLIK
Sbjct: 278  NKWFYHTDSCFTQPSDKLRFLNKACVVKNPEQDIAADGTAADPWSLCTVEQVEELKTLIK 337

Query: 890  VIPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDR 949
            VIPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDR
Sbjct: 338  VIPIWSTGVMMSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVILYDR 397

Query: 950  AILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAVV 1009
            AILPLASKIRGKPVHFGVKSRMGAGL+CSAMSMALSAIVENIRR KAI QGIVDD +AVV
Sbjct: 398  AILPLASKIRGKPVHFGVKSRMGAGLVCSAMSMALSAIVENIRRGKAIRQGIVDDPNAVV 457

Query: 1010 DMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIM 1069
            +MSA+WL+PQHCLNGLAEALNAI QTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIM
Sbjct: 458  NMSAMWLVPQHCLNGLAEALNAIAQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAIM 517

Query: 1070 STVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRTA 1129
            STVDNVTSKGGKESWVSKNIN+GHFE YYWLL ILSVINVLYYVVCSWAYGPSVDQRRTA
Sbjct: 518  STVDNVTSKGGKESWVSKNINRGHFENYYWLLTILSVINVLYYVVCSWAYGPSVDQRRTA 577

Query: 1130 MDDGKISSNEDELSMLDARVKEEEGELHKVKELEA 1162
            MDDGKISS+E+ELSML+ARVKEE GEL K KEL+A
Sbjct: 578  MDDGKISSHEEELSMLEARVKEE-GELQKPKELQA 611

BLAST of Chy1G020850 vs. TAIR 10
Match: AT1G52190.1 (Major facilitator superfamily protein )

HSP 1 Score: 708.8 bits (1828), Expect = 7.4e-204
Identity = 355/569 (62.39%), Postives = 448/569 (78.73%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE+ EKV SYGL+PNMI+YL++DY  G AKG N+LF WSAA NF PLLGAFL+DSYLGRF
Sbjct: 34   NEAFEKVASYGLLPNMIMYLIRDYRFGVAKGTNVLFMWSAASNFTPLLGAFLSDSYLGRF 93

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPET-CRSPTAAQMALLAVALTLMSI 709
            LTI   S+++FLGM+LLWLTAM+P  KP  CD     + C S TA+Q+ALL  A  L+SI
Sbjct: 94   LTISIASLSSFLGMVLLWLTAMLPQVKPSPCDPTAAGSHCGSSTASQLALLYSAFALISI 153

Query: 710  GAGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGW 769
            G+GG+RPC+LAFGADQ+D ++NP N+R+LE FFGWYYAS++ +VLIA TGIVYIQ+H+GW
Sbjct: 154  GSGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSAVAVLIAFTGIVYIQEHLGW 213

Query: 770  KVGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPAST 829
            K+GFGVPA LML A +LF  AS +YV +  TKSLF+  AQ +VAA+K RK  LP    S 
Sbjct: 214  KIGFGVPAVLMLIAALLFILASPLYVTRGVTKSLFTGLAQAIVAAYKKRKLSLPDHHDSF 273

Query: 830  KWFYH-KDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIK 889
              +YH KDS    PS KLRFLNKAC++ N E++I  DG A +PW LCT ++VEELK LIK
Sbjct: 274  DCYYHMKDSEIKAPSQKLRFLNKACLISNREEEIGSDGFALNPWRLCTTDKVEELKALIK 333

Query: 890  VIPIWSTGVMMSINISQSSFPLLQAKSMDRHIS---STFQIPAGSFGTFVIITIVIWVIL 949
            VIPIWSTG+MMSIN SQSSF LLQA SMDR +S   S+FQ+PAGSFG F II + +WVIL
Sbjct: 334  VIPIWSTGIMMSINTSQSSFQLLQATSMDRRLSRHGSSFQVPAGSFGMFTIIALALWVIL 393

Query: 950  YDRAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLD 1009
            YDRA++PLASKIRG+P    VK RMG GL  S ++MA+SA+VE+ RR+KAI+QG  ++ +
Sbjct: 394  YDRAVIPLASKIRGRPFRLSVKLRMGLGLFMSFLAMAISAMVESFRRKKAISQGYANNSN 453

Query: 1010 AVVDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLAS 1069
            AVVD+SA+WL+PQ+ L+GLAEAL AIGQTEF+Y+EFPK+MSS+A+SLFGLGMAVA+LLAS
Sbjct: 454  AVVDISAMWLVPQYVLHGLAEALTAIGQTEFFYTEFPKSMSSIAASLFGLGMAVASLLAS 513

Query: 1070 AIMSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQR 1129
             +++ V+ +TS+ GKESWVS NINKGH+  YYW+LAI+S INV+YYV+CSW+YGP VDQ 
Sbjct: 514  VVLNAVNELTSRNGKESWVSDNINKGHYNYYYWVLAIMSFINVIYYVICSWSYGPLVDQV 573

Query: 1130 RTAMDDGKISSNEDELSMLDARVKEEEGE 1154
            R    +G+++   +E  ++D   K  E E
Sbjct: 574  R----NGRVNGVREEEELIDIVGKGFEKE 598

BLAST of Chy1G020850 vs. TAIR 10
Match: AT3G16180.1 (Major facilitator superfamily protein )

HSP 1 Score: 681.8 bits (1758), Expect = 9.7e-196
Identity = 340/556 (61.15%), Postives = 426/556 (76.62%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE  EKV SYGL+ NMILYLM DY LG  KG  +LF W AA NFMPL+GAFL+DSYLGRF
Sbjct: 36   NEGFEKVASYGLLQNMILYLMSDYRLGLVKGQTVLFMWVAATNFMPLVGAFLSDSYLGRF 95

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
            LTI   S+++ LGM++LWLTAM+P  KP  C       C S T++Q+ALL  A  L+SIG
Sbjct: 96   LTIVIASLSSLLGMVVLWLTAMLPQVKPSPCVATAGTNCSSATSSQLALLYTAFALISIG 155

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            +GG+RPC+LAFGADQ+D ++NP N+R+LE FFGWYYAS+S +VLIA T IVYIQDH+GWK
Sbjct: 156  SGGIRPCSLAFGADQLDNKENPKNERVLESFFGWYYASSSVAVLIAFTVIVYIQDHLGWK 215

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            +GFG+PA LML A  LF  AS +YVK+  +KSLF+  AQVV AA+  R   LP    S  
Sbjct: 216  IGFGIPAILMLLAGFLFVFASPLYVKRDVSKSLFTGLAQVVAAAYVKRNLTLPDHHDSRD 275

Query: 830  WFYH-KDSLFTQPSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTLIKV 889
             +Y  KDS    PSDKLRFLNKAC + N ++D+  DG A + W LCT +QVE+LK L+KV
Sbjct: 276  CYYRLKDSELKAPSDKLRFLNKACAISNRDEDLGSDGLALNQWRLCTTDQVEKLKALVKV 335

Query: 890  IPIWSTGVMMSINISQSSFPLLQAKSMDRHIS--STFQIPAGSFGTFVIITIVIWVILYD 949
            IP+WSTG+MMSIN+SQ+SF LLQAKSMDR +S  STFQIPAGSFG F II ++ WV+LYD
Sbjct: 336  IPVWSTGIMMSINVSQNSFQLLQAKSMDRRLSSNSTFQIPAGSFGMFTIIALISWVVLYD 395

Query: 950  RAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDLDAV 1009
            RAILPLASKIRG+PV   VK RMG GL  S ++MA+SA VE+ RR+ AI+QG+ +D ++ 
Sbjct: 396  RAILPLASKIRGRPVRVNVKIRMGLGLFISFLAMAVSATVEHYRRKTAISQGLANDANST 455

Query: 1010 VDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLASAI 1069
            V +SA+WL+PQ+ L+GLAEAL  IGQTEF+Y+EFPK+MSS+A+SLFGLGMAVAN+LAS I
Sbjct: 456  VSISAMWLVPQYVLHGLAEALTGIGQTEFFYTEFPKSMSSIAASLFGLGMAVANILASVI 515

Query: 1070 MSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQRRT 1129
            ++ V N +SK G  SW+  NINKGH++ YYW+LAILS +NV+YYVVCSW+YGP+VDQ R 
Sbjct: 516  LNAVKN-SSKQGNVSWIEDNINKGHYDYYYWVLAILSFVNVIYYVVCSWSYGPTVDQVRN 575

Query: 1130 AMDDGKISSNEDELSM 1143
               +G     E+ + +
Sbjct: 576  DKVNGMRKEEEEVIKL 590

BLAST of Chy1G020850 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 397.5 bits (1020), Expect = 3.7e-110
Identity = 217/559 (38.82%), Postives = 334/559 (59.75%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE+LE++GS GL+ N ++YL K ++L      N++  WS   N  PL+GA+++D+Y+GRF
Sbjct: 64   NETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVGAYISDTYVGRF 123

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
             TI F S AT LG++ + LTA  P   P  C+   P +C  P   Q+ +L + L  +S+G
Sbjct: 124  KTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGLCFLSVG 183

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            +GG+RPC++ FG DQ D+R     K +   FF WYY + +  ++I  T +VYIQD V W 
Sbjct: 184  SGGIRPCSIPFGVDQFDQRTEEGVKGVAS-FFNWYYMTFTVVLIITQTVVVYIQDQVSWI 243

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            +GF +P GLM  A V+FFA    YV  K   S+FS  AQV+VAA K RK  LP     T 
Sbjct: 244  IGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAEDDGTV 303

Query: 830  WFYH---KDSLFTQ--PSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKT 889
             +Y    K S+ ++   S++ R L+KA VV   E D+  +G  AD W LC+V++VEE+K 
Sbjct: 304  TYYDPAIKSSVLSKLHRSNQFRCLDKAAVV--IEGDLTPEGPPADKWRLCSVQEVEEVKC 363

Query: 890  LIKVIPIWSTGVM-MSINISQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVI 949
            LI+++PIWS G++ ++   +Q +F + QA  MDR++   F+IPAGS     ++TI I++ 
Sbjct: 364  LIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTIGIFLP 423

Query: 950  LYDRAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDL 1009
             YDR  +P   +I G      +  R+G G++ +  SM ++ IVE +RR ++I  G   D 
Sbjct: 424  FYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAG---DP 483

Query: 1010 DAVVDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLA 1069
              +  MS  WL PQ  L GL EA N IGQ EF+ S+FP+ M S+A+SLF L  A ++ L+
Sbjct: 484  TGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSYLS 543

Query: 1070 SAIMSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAYGPSVDQ 1129
            S +++ V   +    +  W++KN+N G  + +Y+L+A+L V+N++Y+  C+  Y   V  
Sbjct: 544  SFLVTVVHKFSGGHDRPDWLNKNLNAGKLDYFYYLIAVLGVVNLVYFWYCARGYRYKVG- 603

Query: 1130 RRTAMDDGKISSNEDELSM 1143
                ++D +   + D++ M
Sbjct: 604  --LPIEDFEEDKSSDDVEM 613

BLAST of Chy1G020850 vs. TAIR 10
Match: AT3G47960.1 (Major facilitator superfamily protein )

HSP 1 Score: 369.8 bits (948), Expect = 8.2e-102
Identity = 210/562 (37.37%), Postives = 332/562 (59.07%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE+ EK+G  G + N+++YL   +NL       I+  +S  INF   + AFL D+Y GR+
Sbjct: 74   NETFEKLGIIGTLSNLLVYLTSVFNLKSYTAATIINAFSGTINFGTFIAAFLCDTYFGRY 133

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
             T+    IA FLG  ++ LTA +PS  P  C   +  +C  P+  Q+  L + L  + +G
Sbjct: 134  KTLSVAVIACFLGSFVILLTAAIPSLHPVACG--NKISCEGPSVGQILFLLMGLGFLVVG 193

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            AGG+RPC LAFGADQ + + + + K+ +  FF WY+ + +F+ +I+LT +VYIQ +V W 
Sbjct: 194  AGGIRPCNLAFGADQFNPK-SESGKKGINSFFNWYFFTFTFAQIISLTAVVYIQSNVSWT 253

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            +G  +P  LM  A V+FFA   +YVK KA+ S  +  A+V+ AA K R    P       
Sbjct: 254  IGLIIPVALMFLACVIFFAGDRLYVKVKASGSPLAGIARVIAAAIKKRGLK-PVKQPWVN 313

Query: 830  WFYHKDSLFTQP----SDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKTL 889
             + H  S +       +D+ RFL+KA ++  PE+ +  DGTA+DPW LCT++QVEE+K +
Sbjct: 314  LYNHIPSNYANTTLKYTDQFRFLDKAAIM-TPEEKLNSDGTASDPWKLCTLQQVEEVKCI 373

Query: 890  IKVIPIW--STGVMMSINISQSSFPLLQAKSMDRHI-SSTFQIPAGSFGTFVIITIVIWV 949
            ++VIPIW  ST   ++I I Q ++P+ QA   DR + S  F+IPA ++  F++  + +++
Sbjct: 374  VRVIPIWFASTIYYLAITI-QMTYPVFQALQSDRRLGSGGFRIPAATYVVFLMTGMTVFI 433

Query: 950  ILYDRAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQ---GI 1009
            I YDR ++P   ++ G      +  R+GAG   + MS+ +S  +E  RR  A+ +   G+
Sbjct: 434  IFYDRVLVPSLRRVTGLETGISLLQRIGAGFTFAIMSLLVSGFIEERRRNFALTKPTLGM 493

Query: 1010 VDDLDAVVDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVA 1069
                  +  MSALWLIPQ  L G+AEA  AIGQ EFYY +FP+ M S A S+F +G  V+
Sbjct: 494  APRTGEISSMSALWLIPQLTLAGIAEAFAAIGQMEFYYKQFPENMKSFAGSIFYVGAGVS 553

Query: 1070 NLLASAIMSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCS--WAY 1129
            + LAS ++STV   T+     +W+++++NK   + +Y++L  L V+N+ Y+++ +  + Y
Sbjct: 554  SYLASFLISTVHRTTAHSPSGNWLAEDLNKAKLDYFYFMLTGLMVVNMAYFLLMARWYRY 613

Query: 1130 GPSVDQRRTAMDDGKISSNEDE 1140
                D+     D  +I +NE+E
Sbjct: 614  KGGNDE-----DITEIETNEEE 624

BLAST of Chy1G020850 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 365.9 bits (938), Expect = 1.2e-100
Identity = 196/534 (36.70%), Postives = 316/534 (59.18%), Query Frame = 0

Query: 590  NESLEKVGSYGLMPNMILYLMKDYNLGFAKGNNILFFWSAAINFMPLLGAFLADSYLGRF 649
            NE+LEK+GS G+  N +LYL   +++   +  N+ + W    NF PLLGA ++D+Y+GRF
Sbjct: 27   NETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLGALISDAYIGRF 86

Query: 650  LTIGFGSIATFLGMLLLWLTAMVPSTKPPVCDQLHPETCRSPTAAQMALLAVALTLMSIG 709
             TI + S+ + LG++ + LTA +P   PP C+  HP+ C  P   Q+ +L + L  +SIG
Sbjct: 87   KTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGILFLGLGFLSIG 146

Query: 710  AGGVRPCTLAFGADQIDRRDNPNNKRMLERFFGWYYASASFSVLIALTGIVYIQDHVGWK 769
            +GG+RPC++ FG DQ D+R     K +   FF WYY + +  ++ + T +VY+Q  V W 
Sbjct: 147  SGGIRPCSIPFGVDQFDQRTEQGLKGVAS-FFNWYYLTLTMVLIFSHTVVVYLQT-VSWV 206

Query: 770  VGFGVPAGLMLFATVLFFAASSIYVKQKATKSLFSSFAQVVVAAFKNRKFPLPTSPASTK 829
            +GF +P  LM  A VLFF     YV  K   S+FS  A+V+VAA K R   +      T+
Sbjct: 207  IGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDLKISLVDDGTE 266

Query: 830  WFYH---KDSLFTQ--PSDKLRFLNKACVVKNPEQDIAGDGTAADPWSLCTVEQVEELKT 889
             +Y    K  + ++   +D+ +FL+KA V+   + D+  +G  A+ W LC++++VEE+K 
Sbjct: 267  EYYEPPVKPGVLSKLPLTDQFKFLDKAAVIL--DGDLTSEGVPANKWRLCSIQEVEEVKC 326

Query: 890  LIKVIPIWSTGVMMSINI-SQSSFPLLQAKSMDRHISSTFQIPAGSFGTFVIITIVIWVI 949
            LI+V+P+WS G++  + + +Q++F + QA  MDRH+   F+IPA S      ITI IWV 
Sbjct: 327  LIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVISYITIGIWVP 386

Query: 950  LYDRAILPLASKIRGKPVHFGVKSRMGAGLICSAMSMALSAIVENIRRRKAIAQGIVDDL 1009
            +Y+  ++P   ++R       +  RMG G++ + +SM  +  VE +RR +A         
Sbjct: 387  IYEHLLVPFLWRMR--KFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTRA--------- 446

Query: 1010 DAVVDMSALWLIPQHCLNGLAEALNAIGQTEFYYSEFPKTMSSVASSLFGLGMAVANLLA 1069
              +  MS  WL     L GL E+ N IG  EF+ S+FP+ M S+A+SLF L  A AN L+
Sbjct: 447  TEMTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAAANYLS 506

Query: 1070 SAIMSTVDNVTSKGGKESWVSKNINKGHFEKYYWLLAILSVINVLYYVVCSWAY 1118
            S +++TV  V+       W++K++++G  + +Y+L+A+L V+N++Y+  C+  Y
Sbjct: 507  SLLVTTVHKVSGTKDHPDWLNKDLDRGKLDYFYYLIAVLGVVNLVYFWYCAHRY 545