Chy1G017760 (gene) Cucumber (hystrix) v1

Overview


Name	Chy1G017760
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	nifU-like protein 1, chloroplastic
Location	chrH01: 23394596 .. 23396762 (+)
RNA-Seq Expression	Chy1G017760
Synteny	Chy1G017760

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCCTTAACTACTTCCGGCCTCCTCAAAACCCCTCTTAAGAATTACCAATCTCCTGCAAATTATCTCCAATATCCACCTTTTGCTTATGGGCAACATCAACTACCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGACCTCCTCACCACGGCTGTACTCCGCTCAGAAATTTGAACTGACCATTGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGACGGAGTAGTTTCCCTCAAACTCGTTGGTGGGTAGATTTCTTGTCTCGTTGAATCGAATGGGTTTGTTTTAATTTCCCTTGTTAATTTAATTTTTTGTGTTGAAATTTTTGCTACTTAACAATTGGTTGCTGTTGGGGTATGTTCTTTGGTGTTTCTATAGTTTAGCAAACTGTGTTGAATATCTGTTACACATTATGGTATTTTGTAGTATCTGCACTTTAGAGATTTGAGTCTGATTCATTTCTTTCATCGAAGCAATCCTGGGATCGTATGGCTTAGGACAAAGTCGACATCTTTGTGATTCTCGGTCTGAGTATGCCCCTTCAGCCCATCCCTTTTTACATGGCCAAATAGTTGGTGTACGCTTTCTCGACGTTGGCCCGATAGTCGTGATTCTTTTTTCTCAAAAAGAATGATTTCGATCTTCTTCTTTCTTAGCATTACTGTAAACCATTTGATCTTAGTCGTCTTGTGTGGAAGGAGCGAGATGACAGAGATTTTGCTCTTCTCACCCCTGTCCGACCACCCGATTGGTGAAGACTCGGAATTAACCACCCCGCTTTGGGAGACACCGAAGACTTTACTGGATGTCTTGATCCATCCGTAGTCTGCTAATCTGATCCCAACGTCTTTAATTCTGACGAGCTTGTCTCGGAGAGATCTCTATCTTTTGTTTGTTACACAACACTGAAGGCTCCACTAGAAAAGGAAGGAAAAAGGAAAATACCTCCAATGATAAATCCCAAGATCAGGATTAGTAGTGCTGCAGTTCTATTTACAATCTTAAACTTTCCTGACTACAATCTTCCTCCATTAGCACTGAATCTATTGGAAATTTTGAAAAGTTTAGACTTGGGCAGATGGATATGATTTTCCTTTCTTTATATGAGGGTCTTTCCAATGAAAAGGATTTACCAGAGAATAGATGATTTGATATCTATCTTTAAAGAAAACCACGAAAAGGATCCTTAATTTCACTTGTCTGTGAACACTTTTAACCAATCTTGTGGGAGATTTGGTATATATCTAATGGGAATGTTTCTCAGGACATGGGTGGGGTTCTCATGACATTTGAAGGGACTGCAGACTAATGTAGCCTTTCGGTTTCTTTCCTGCATATACTAGTTGGAGTACATTTGTTTTGTTGTCTTGGTCTAGGTAAGTTTTGAACAGTACTATCATCTTGTACATGTTACTGGTGAGTTTTATCTGTTTCTTATCAAGTAGGGTGAAAAAGGTCTCCTTGAACTAAATGGCAAAAGGAAGTCTTGTAACCCTCACTTTCAACTAATAAGGGTGTGAGTAAGAACTTTGTTGATTTGATGTTTAACTGCTTTGTATTCCCATGAAGTCATGAACAAAAAGTAATAATGATAAACATTTTTAACTAAAAATGTTTGAATCGATCTTCAGGAGCATGCGGTAGCTGCCCTAGCTCAACAACCACCATGAAGATGGGGATCGAACGTGTACTAAAGGAGAAGTTTGGAGATTCGGTGAAGGAAATATGCCAAGTATATGATGAAGAACCAAAGGAGACTACACCTGAGGTGAGTTTTCTGCAGATGAGCAAATACAAAATTGGCTGATGTCAAAATTCAAACAAAAACAAAGTGAGCTGAATTAGTTAGGTAATTAGTATTAATCCTGATGGTGCACTAATGCAGGCAGTAAACAGTCACCTTGACATACTGAGGCCAGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCGATTAATGGTGGCAATTGCCTGGTCAAGTATGAGGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

mRNA sequence

ATGGCGTCCTTAACTACTTCCGGCCTCCTCAAAACCCCTCTTAAGAATTACCAATCTCCTGCAAATTATCTCCAATATCCACCTTTTGCTTATGGGCAACATCAACTACCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGACCTCCTCACCACGGCTGTACTCCGCTCAGAAATTTGAACTGACCATTGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGACGGAGTAGTTTCCCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCTAGCTCAACAACCACCATGAAGATGGGGATCGAACGTGTACTAAAGGAGAAGTTTGGAGATTCGGTGAAGGAAATATGCCAAGTATATGATGAAGAACCAAAGGAGACTACACCTGAGGCAGTAAACAGTCACCTTGACATACTGAGGCCAGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCGATTAATGGTGGCAATTGCCTGGTCAAGTATGAGGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

Coding sequence (CDS)

ATGGCGTCCTTAACTACTTCCGGCCTCCTCAAAACCCCTCTTAAGAATTACCAATCTCCTGCAAATTATCTCCAATATCCACCTTTTGCTTATGGGCAACATCAACTACCCGTTTCCCTCAAAAGGGCTTTTCCCGGACGAGCCATTAGAGCTTCACCTTCGAATTCGGGTCCATCCACGACCTCCTCACCACGGCTGTACTCCGCTCAGAAATTTGAACTGACCATTGGAAACGTGGACTTGGTTCTTGAGGACGTAAGGCCTTATCTAATTGCAGATGGAGGAAATGTCGATGTTGTTTCTGTTGAAGACGGAGTAGTTTCCCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCTAGCTCAACAACCACCATGAAGATGGGGATCGAACGTGTACTAAAGGAGAAGTTTGGAGATTCGGTGAAGGAAATATGCCAAGTATATGATGAAGAACCAAAGGAGACTACACCTGAGGCAGTAAACAGTCACCTTGACATACTGAGGCCAGCCATAAGGAACTACGGTGGGAGCGTGGAAGTAATATCGATTAATGGTGGCAATTGCCTGGTCAAGTATGAGGGTCCTGAATCTATTGGTACAGGAGTTAAAGCAGCCATTAAGGAGAGATTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAA

Protein sequence

MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPSTTSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEVISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS*

Homology

[Close]

BLAST of Chy1G017760 vs. ExPASy Swiss-Prot
Match: Q93W77 (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 1.5e-62
Identity = 138/230 (60.00%), Postives = 164/230 (71.30%), Query Frame = 0


Query: 1   MASLTTS------GLLKTPLKNYQSPANYLQYPPFA-YGQHQLPVSLKRAFPGRAIRASP 60
           MASL TS       L+K+       P    Q P F  +   +  +S    F   AI  S 
Sbjct: 2   MASLATSISGSFRILVKSSSTRNGFPVISDQNPSFVLFANKRRHISRTAIFHRSAISGSS 61

Query: 61  SNSGPSTTS---SPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLK 120
                S  +   S  LYSAQ F+LT  NVDLVLEDVRP+LI+DGGNVDVVSVEDGVVSLK
Sbjct: 62  QGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSLK 121

Query: 121 LVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPA 180
           L GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV+DEE K+ T EAVN+HLDILRPA
Sbjct: 122 LQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEVKQITVEAVNAHLDILRPA 181

Query: 181 IRNYGGSVEVISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           I+NYGGSVEV+S+ G +C+VKY GPESIG G++AAIKE+F DI+NV F+S
Sbjct: 182 IKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKDISNVTFTS 231

[Close]

BLAST of Chy1G017760 vs. ExPASy Swiss-Prot
Match: Q84LK7 (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 9.4e-20
Identity = 64/152 (42.11%), Postives = 87/152 (57.24%), Query Frame = 0


Query: 68  SAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKM 127
           +A +  LT GNV+ VL+ VRPYL ADGG+V +  +   VV LKL GACGSCPSS  T+K 
Sbjct: 69  TAVQLPLTAGNVESVLDQVRPYLTADGGDVALHEIAGNVVRLKLQGACGSCPSSLITIKR 128

Query: 128 GIERVLKEKFGDSVKEICQVYDEEP-KETTPEAVNSHLDILRPAIRNY-GGSVEVISING 187
           GIER L EK  D V  +  V D+E   E   E V   L+ +RP +    GG ++ + I G
Sbjct: 129 GIERRLMEKIPD-VAAVEPVTDKETGLELNEENVEKVLNEIRPYLAGTGGGGLQFLMIKG 188

Query: 188 GNCLVKYEGPESIGTGVKAAI----KERFPDI 214
               V+  GP ++   V+ A+    +E+ P I
Sbjct: 189 PIVKVRLTGPAAVVRTVRIAVSKKLREKIPSI 219

[Close]

BLAST of Chy1G017760 vs. ExPASy Swiss-Prot
Match: Q93W20 (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.2e-19
Identity = 57/148 (38.51%), Postives = 84/148 (56.76%), Query Frame = 0


Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRP-AIRNYGGSVEVISINGGNCLVKY 193
            EK  + V       +E   E   E +   L+ +RP  I    GS++++ I      ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGVKAAIKERFPDITNV 217
            GP     ++   V   ++E+ P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

[Close]

BLAST of Chy1G017760 vs. ExPASy Swiss-Prot
Match: Q84RQ7 (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.4e-17
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  VV LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KETTPEAVNSHLDILRPAIRNY-GGSVEVISINGGNCLV 193
           ++K  + +  + Q  + E    E   E +   L  LRP +    GG +E++ I+G    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGVKAAIKER 210
           +  GP +    V+ A+ ++
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

[Close]

BLAST of Chy1G017760 vs. ExPASy Swiss-Prot
Match: O32119 (Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224308 GN=yutI PE=3 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.5e-13
Identity = 37/70 (52.86%), Postives = 53/70 (75.71%), Query Frame = 0


Query: 79  VDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVLKEKFG 138
           V  VL+ +RP+L+ DGG+ ++V V++G+V L+L+GACGSCPSST T+K GIER L E+  
Sbjct: 43  VQEVLDKLRPFLLRDGGDCELVDVDEGIVKLRLLGACGSCPSSTITLKAGIERALLEEV- 102

Query: 139 DSVKEICQVY 149
             V E+ QV+
Sbjct: 103 PGVVEVEQVF 111

[Close]

BLAST of Chy1G017760 vs. ExPASy TrEMBL
Match: A0A1S3BQX1 (nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 4.3e-116
Identity = 214/220 (97.27%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKN QSPANY QYPPFAYGQHQLPVSLKRAFPGRA+RASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSP LYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of Chy1G017760 vs. ExPASy TrEMBL
Match: A0A0A0KB98 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G412870 PE=3 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 7.3e-116
Identity = 216/220 (98.18%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKN QSPAN LQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           TSSP LYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGG+CLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of Chy1G017760 vs. ExPASy TrEMBL
Match: A0A5A7TT28 (NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001900 PE=3 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 1.6e-115
Identity = 213/220 (96.82%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKN QSPANY QYPPFAYGQHQLPVSLKRAFPGRA+RASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSP LYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISINGG+CLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of Chy1G017760 vs. ExPASy TrEMBL
Match: A0A6J1CRB9 (nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014036 PE=3 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.1e-103
Identity = 196/219 (89.50%), Postives = 203/219 (92.69%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTP    Q PANY QYPP A+ QHQ P SLKR  P R I+AS SNSGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSP LYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLD+LRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 220
           ISI+GG+CLVKYEGPESIGTG+KAAIKERFPDITNVVFS
Sbjct: 181 ISIDGGDCLVKYEGPESIGTGIKAAIKERFPDITNVVFS 219

[Close]

BLAST of Chy1G017760 vs. ExPASy TrEMBL
Match: A0A6J1JN01 (nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 PE=3 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 1.0e-101
Identity = 192/220 (87.27%), Postives = 204/220 (92.73%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLT SGLLKTPL   Q+ AN+ QYPP A+GQHQ+PVSL RA P ++IRAS SNSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSP LYSAQKFELTI NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           ISI+GGNC+V YEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of Chy1G017760 vs. NCBI nr
Match: XP_008451442.1 (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo])

HSP 1 Score: 427 bits (1099), Expect = 9.32e-151
Identity = 214/220 (97.27%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKN QSPANY QYPPFAYGQHQLPVSLKRAFPGRA+RASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSP LYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220
           ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of Chy1G017760 vs. NCBI nr
Match: XP_004135981.1 (nifU-like protein 1, chloroplastic [Cucumis sativus])

HSP 1 Score: 427 bits (1097), Expect = 1.88e-150
Identity = 216/220 (98.18%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKN QSPAN LQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANSLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           TSSP LYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  TSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220
           ISINGG+CLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of Chy1G017760 vs. NCBI nr
Match: KAA0046713.1 (nifU-like protein 1 [Cucumis melo var. makuwa] >TYK18249.1 nifU-like protein 1 [Cucumis melo var. makuwa])

HSP 1 Score: 426 bits (1094), Expect = 5.39e-150
Identity = 213/220 (96.82%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPLKN QSPANY QYPPFAYGQHQLPVSLKRAFPGRA+RASPSNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSP LYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220
           ISINGG+CLVKYEGPESIGTGVKAAIKERFPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of Chy1G017760 vs. NCBI nr
Match: XP_038898528.1 (nifU-like protein 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 394 bits (1011), Expect = 2.42e-137
Identity = 200/219 (91.32%), Postives = 207/219 (94.52%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTPL   Q+PANY QY PFA+GQHQLP+SLKRA P RAIRAS SNS PST
Sbjct: 1   MASLTTSGLLKTPLSYSQTPANYRQYTPFAHGQHQLPISLKRASPRRAIRASASNSSPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSP LYSAQKFELTI NVDLVLEDVRPYLIADGGNVDVVS+EDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIRNVDLVLEDVRPYLIADGGNVDVVSIEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDE PKETTPEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEVPKETTPEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 219
           ISINGG+CLVKYEGPESIGTGVKAAIKERFPDIT+VVFS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITHVVFS 219

[Close]

BLAST of Chy1G017760 vs. NCBI nr
Match: XP_022144330.1 (nifU-like protein 1, chloroplastic [Momordica charantia])

HSP 1 Score: 384 bits (987), Expect = 1.18e-133
Identity = 196/219 (89.50%), Postives = 203/219 (92.69%), Query Frame = 0


Query: 1   MASLTTSGLLKTPLKNYQSPANYLQYPPFAYGQHQLPVSLKRAFPGRAIRASPSNSGPST 60
           MASLTTSGLLKTP    Q PANY QYPP A+ QHQ P SLKR  P R I+AS SNSGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  TSSPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120
           +SSP LYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLD+LRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEV 180

Query: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFS 219
           ISI+GG+CLVKYEGPESIGTG+KAAIKERFPDITNVVFS
Sbjct: 181 ISIDGGDCLVKYEGPESIGTGIKAAIKERFPDITNVVFS 219

[Close]

BLAST of Chy1G017760 vs. TAIR 10
Match: AT4G01940.1 (NFU domain protein 1 )

HSP 1 Score: 240.7 bits (613), Expect = 1.1e-63
Identity = 138/230 (60.00%), Postives = 164/230 (71.30%), Query Frame = 0


Query: 1   MASLTTS------GLLKTPLKNYQSPANYLQYPPFA-YGQHQLPVSLKRAFPGRAIRASP 60
           MASL TS       L+K+       P    Q P F  +   +  +S    F   AI  S 
Sbjct: 2   MASLATSISGSFRILVKSSSTRNGFPVISDQNPSFVLFANKRRHISRTAIFHRSAISGSS 61

Query: 61  SNSGPSTTS---SPRLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLK 120
                S  +   S  LYSAQ F+LT  NVDLVLEDVRP+LI+DGGNVDVVSVEDGVVSLK
Sbjct: 62  QGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLEDVRPFLISDGGNVDVVSVEDGVVSLK 121

Query: 121 LVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRPA 180
           L GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV+DEE K+ T EAVN+HLDILRPA
Sbjct: 122 LQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQVFDEEVKQITVEAVNAHLDILRPA 181

Query: 181 IRNYGGSVEVISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 221
           I+NYGGSVEV+S+ G +C+VKY GPESIG G++AAIKE+F DI+NV F+S
Sbjct: 182 IKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIKEKFKDISNVTFTS 231

[Close]

BLAST of Chy1G017760 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 98.2 bits (243), Expect = 8.7e-21
Identity = 57/148 (38.51%), Postives = 84/148 (56.76%), Query Frame = 0


Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKETTPEAVNSHLDILRP-AIRNYGGSVEVISINGGNCLVKY 193
            EK  + V       +E   E   E +   L+ +RP  I    GS++++ I      ++ 
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGVKAAIKERFPDITNV 217
            GP     ++   V   ++E+ P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

[Close]

BLAST of Chy1G017760 vs. TAIR 10
Match: AT4G25910.1 (NFU domain protein 3 )

HSP 1 Score: 89.7 bits (221), Expect = 3.1e-18
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  VV LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KETTPEAVNSHLDILRPAIRNY-GGSVEVISINGGNCLV 193
           ++K  + +  + Q  + E    E   E +   L  LRP +    GG +E++ I+G    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 KYEGPESIGTGVKAAIKER 210
           +  GP +    V+ A+ ++
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

[Close]

BLAST of Chy1G017760 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 82.8 bits (203), Expect = 3.8e-16
Identity = 40/68 (58.82%), Postives = 52/68 (76.47%), Query Frame = 0


Query: 74  LTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  +V +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSV 142
            EK  + V
Sbjct: 145 MEKIPEIV 152

[Close]

BLAST of Chy1G017760 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 54.7 bits (130), Expect = 1.1e-07
Identity = 32/85 (37.65%), Postives = 50/85 (58.82%), Query Frame = 0


Query: 75  TIGNVDLVLED-VRPYLIADGGNVDVVSV--EDGVVSLKLVGACGSCPSSTTTMKMGIER 134
           T+  +  +LE  +RP +  DGG+++      E G+V L++ GAC  CPSS+ T+K GIE 
Sbjct: 192 TVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSSSVTLKSGIEN 251

Query: 135 VLKEKFGDSVKEICQVYDEEPKETT 157
           +L   +   VK + Q +D E +E T
Sbjct: 252 MLMH-YVSEVKGVEQEFDGEDEEGT 275

The following BLAST results are available for this feature:

BLAST of Chy1G017760 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93W77	1.5e-62	60.00	NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1...	[more]
Q84LK7	9.4e-20	42.11	NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=N...	[more]
Q93W20	1.2e-19	38.51	NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1...	[more]
Q84RQ7	4.4e-17	40.29	NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2...	[more]
O32119	6.5e-13	52.86	Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224...	[more]

BLAST of Chy1G017760 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BQX1	4.3e-116	97.27	nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 ...	[more]
A0A0A0KB98	7.3e-116	98.18	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G412870 PE=3 SV=1	[more]
A0A5A7TT28	1.6e-115	96.82	NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G...	[more]
A0A6J1CRB9	4.1e-103	89.50	nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101403...	[more]
A0A6J1JN01	1.0e-101	87.27	nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 P...	[more]

BLAST of Chy1G017760 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008451442.1	9.32e-151	97.27	PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo]	[more]
XP_004135981.1	1.88e-150	98.18	nifU-like protein 1, chloroplastic [Cucumis sativus]	[more]
KAA0046713.1	5.39e-150	96.82	nifU-like protein 1 [Cucumis melo var. makuwa] >TYK18249.1 nifU-like protein 1 [...	[more]
XP_038898528.1	2.42e-137	91.32	nifU-like protein 1, chloroplastic [Benincasa hispida]	[more]
XP_022144330.1	1.18e-133	89.50	nifU-like protein 1, chloroplastic [Momordica charantia]	[more]

BLAST of Chy1G017760 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G01940.1	1.1e-63	60.00	NFU domain protein 1	[more]
AT5G49940.1	8.7e-21	38.51	NIFU-like protein 2	[more]
AT4G25910.1	3.1e-18	40.29	NFU domain protein 3	[more]
AT5G49940.2	3.8e-16	58.82	NIFU-like protein 2	[more]
AT3G20970.1	1.1e-07	37.65	NFU domain protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 71..155 e-value: 8.0E-27 score: 95.1
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 156..218 e-value: 6.0E-10 score: 41.1
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 77..149
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 155..217
IPR001075	NIF system FeS cluster assembly, NifU, C-terminal	PFAM	PF01106	NifU	coord: 80..139 e-value: 9.6E-23 score: 80.1 coord: 162..216 e-value: 2.7E-4 score: 21.0
None	No IPR available	PANTHER	PTHR11178:SF15	NIFU-LIKE PROTEIN 1, CHLOROPLASTIC	coord: 54..219
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 54..219

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy1G017760.1	Chy1G017760.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding