Chy1G016420 (gene) Cucumber (hystrix) v1

Overview
NameChy1G016420
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionE3 ubiquitin-protein ligase MIEL1-like
LocationchrH01: 22388262 .. 22394032 (-)
RNA-Seq ExpressionChy1G016420
SyntenyChy1G016420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCCATTAGACCTCTAGTTTCCATAACTTTCTTGGCTCTTTTCTTCTTCTCCGAGTCCCTACTCACTTCCCATTCTCTTACCGGAATTCCTCACCGGAACATCTTGAACACAGCTGCGCATCGGATTATGGATGGCAGTGTTGAGTACTCCTCCTACCAAATTAGGAAGTCGAATAAGCTTCATATCAAATATAGAAGAAAGCGGCGGAGTCGACCAATACTAGTCAGCAGCAGCAGCGGCAACCATCATAAATCGGCAGCGAATCGGAGCCGAGTGGCGTCCCTTGAAGTTGGATCTATGTTGTGTATCTGCATTTTCTTGGGATTGTTCTTGTGAATATTTTGGAAGCTTTGGACTCTTTGTTCCTTTTTTTTTTCCTTTTTTGTGGTGTGTATATTTAGCTCTAAGATAACGATTTAAAGGGAAGAAAAAAATCAATGACTCTAAAACTTTTGATTTTTCATCTTACTAAAAATGTACTTGGGAAGAATGAAATTGAAAATCAATTACATTATTCAATTTATTGCATAGTGCTTTCTACTATCAACTATCGGTTGAGGTTTGTGGTTTGATTTATCTAAAAATAATAATAATATTAAGTAAAAAAATCAATTTGTCTCTTTCGAAGAAAATTGAGAGGTTGTATTAATTAATTTAAATAATACTCAACTAAGTGTATCAATTTAAACCTCAATTTTGTTAAGTGTATTAATTTACTCATTCTAATATATTATGTTTGAAAAATATCTTGTGTGAAGTTTATAATTAAATTAATTAAGCTTTTAATCTTTTGTAAATCAATAAATCTATGCTCTTAGACAAATTTTCATATAAAAGTTGTATGTGTATTTACTTTAATCTTCTATTTTATAAGTTTAATATTTAAAGAAGTATACATGTATTTGAGAATTAGTCTATGGATGCTTTTCAAAGGTAATCACAATAAAAGTTGTAAACTATTCGTTTACGAAACTCTAAATATTTAATTAACTAAAATTTCAAGCAATATGATATTAGTCCAATTTAATCTGGAGAATAGTGTAAATTGATACCGTTGTGAAATTTTAAAGTTTAAACTGATACAATTATTAATTTTGGAGTTTTAATTTTATACCACTTCAAAATTTATAGTATAAATTGATATTTTTTTAAATAATAAATTTTGATTTTTTTTAACGATATAAAGTAATTAAAAAGGAATAGGAATAAAGAGTGAAAAGATAATCCAAAGTGAAACTTAAAAGTAACTACAAATTTGAAAATTTTTAAATAAGAATGGTTGAAAAAGAATGATACCCACAACAATATTTTAATTTTTTTTTTTTTTTTTTTACTTTTCAATTCTTATCTATATTTTTCTTCTTTTACAAAACAAGAAAGAACAAAGCTTAAATTAATTTGTGTTATTGGCTATTCAGAAAGAGTGAAAAAGTTATATTTATTTTTCAAAGTCAAATCCTTTTTAATGGCAACATCTTACAACTTTGCAATGGCTGCTTCTTGTGATTTTCTATTTTATTAAATTATGAAAACAAAAAAAAATTATAGAAAAAGCCATCTTATAATATGACACAATTATTTCATATTAAATTGATTAGGACTTTCTTCATTTTCTTAAAAAAAAATATATATATATTAATATTGATGTGTACTTGACAATCCACCCCACCACTGTTTTTTCATCATATAATGTGTACTTAAAAAAAGCTTAAGAATATTTTTTAGATAAAATCGTAATTGTTTTCCTCCAAAATAAATGATAAGGTATTTTTTTAACTCATTTTAAAAGTTTTGAACATAAAGTATAAAGTTTATGAATATTTTTTATAATTTAACTAATGAAAAATAGGATAAATTTAATATTTATAAATTGAAAATTTATTGTAAAGTATAAAATTGTGAAAAATATTTACAGAATATAATAGAAAAATTTATGATTCTATCAATTTTTTTTTTTTTTTGTTATTAAAAATTTGATATTTTTTTTAATTTTTATTTAGCAATTTTTGTGGATGATACAAATAATAGAAAAAGAAGAAAGAAAACTTGAAATGAGTTAAAAGGAATTTATTGTCTATATTCGAAAATAGGCTTTTATTTACTCCTTAAAAATTAATTGTAAAATAAATATTCTGTTAATACAAGAATTTGCTGTTGTGTTCTGTATTACCGAATAACTCGAACAGGGAGACGATCTCCAATGGAAGAAGAACACTTCCCTCTGCCCCAATCCGGCCTCAAACAAGATTTCGGAAAATTGCAATATGGGTATCAATCTCTTTCCTATTCCCTTTTTCCTTTTTCCTTTTTCTTCCTCTTCTTCTTCATTCTTATCTTCACCTGTAACTTCTTTTCGATTTCAATTAGATGCGAGCATTACAGGAGACGATGCAAAATCCGAGCTCCATGCTGCGACCGAATCTTCACCTGTCGCCACTGCCACAACGAGGCCATGGTCTTTTCCTCCCCTCTCTCTATGCCTTTTTCCTTGCGTGCGTTTGTTTCTCAGAGATTTATATTGTATGAGGGTTATGTTTTTCCATATGTTGTTAAACTGGAGCTTTCAGTAATTATGATTTTCTGAAACTTTTGTTGATTTTAGACCTCGCTGAGCAATCCCAAGGACCGCCATGAACTTGTTCGCCAGGATATTAGACAAGTCTGTTTGTACGCTGCTCTACTGATTTTTTTTTTAATAGTATTACATTTTGTAAAGAATATATTGTGATGGGGTTTGATTAATTTTGTTGGAATTTGATTCTTTAGGTGGTTTGCTTGATTTGCAACACAGAACAAGAGGTTTGGGGGATTTTTTTTTTTTTTTTTTTGGATTTATTTCAATTCATTGCGTTATATACTCTTGAGCGTTTGAATTCGGTGTGTTGTAGGTTGCTAAGGTTTGTCGCAAGTGTAATGTTAATATGGGAGAATACTTTTGCGACATCTGCAAATTCTATGATGATAATGTATGAGAAGTCAATCCCATTACTTCTTTCTTTTCTTGGATCTATTTTGCTTTCTTTAAGAACTAATTTTCATATCTTAAATGTTGAGCTGAATGGTTTTTGTTACCTTAGATGCTAATAGTTTTTGCTATAAATGCAGACCGAGAAGAAACAGTTTCATTGTGATGCGTGTGGTATCTGTAGGTTAGTAGTGTGCACTTTTCCTGTTCTAATTCTAATGTAAACTCAGCTTTCTGTGTTAAGTTTCTCAAAATAAGTGTTTGTTGTTATATGATTTTTCAGGGTTGGTGGTCGTGAAAACTTCTTTCATTGTGAAAGATGTGGTATGTTACTTAACTCTCTTGATGGTTCACTAGAAATTAGTATTGGGTTATTGACTTGCTGTTTGAAGTAAATGTAGCAGAAGAATATTTTTGGTTAATTGAGATTGAAATAACCTAGTAAAATGTGAACGATTGAACAAATAGACCATTCAGATTGAAATCCATCCATGTAGGCATGAACTTAATTTTGTATTATTAAATATACTGTTGTATTAATTAACTATTTAATCAATTAAAATATAAATAAATGAAATTTTACATCGAACACTAATTGAATGTAATATTGATTATTAAATGGTTTAAAATTAATGAGTATAAAATCAATTAGAATATTAATAATTAATAATAAAATTTGATATTAAATAGTTAAGAATCTAAGATGATATAAAATATCAATTGCATATGAGTAGTTCAAATTTATTAAGTAGGAATTATATGTTCATATTTAACAATTAATTCAAATTGATATACGTCTCACAAAAAATCAAATTGATATTGATTCATTAGTTAATTAGGTTTTATTGTAGGGTATTAAAAGACTCATGTCAAATGGGTATAGGAACATGAGTTTTAAAAAATGAATTCTGTAAAGCAAATAAGGATATTAAATGTCTGTCTGAAAACTTATAGCAAAGCATGTGAAACAAACATGCTGTTAGGGTCTATTTTCCCCTGTTTTTAATGGATAGAGAGATTAACATTTTGTTTTTATGAGTACAGGATCTTGCTATTCTACTCCTTTGCGTGATAACCACAAATGTGTTGAGAACTCAATGAAAAGTTATTGCCCCATCTGTTGGGAGGTTTGTACTTCTAGAATACATATTTACTCTGGGAGGTATAATCTAAATTGTTCTCTTCTAACAATTTTACTGATGCTTGCAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCCTTGTGGTCACACTATACATTGGGAGTGCTTCAGTGAGATGGAATCAATGAATCAGTAATCCAACACTTACTTCAAAATCCAATTTCTGAATCTACTATTAAACATATTTCTATGAACTTTGAATCTACTTAATACAACTCTATTTTCTATTATATCCATTGCATTTTACCAAACTAGTTTTCTGTTTTGAATTACATCGACATTTCCAGTTCTCTAATTTTAATGTAGTACTATCTGTCTCTCAGATACCGTTGTCCAATCTGCTTGAAAACAGTTGTGGACATGTCTGCGAGCTGGGCATTGCTGAAAATGGAGGTGTGTAACATCTTAGTTTAGGCATGATAAAAAATGATATTAAGAAGGCAATTAGACGAACTGGTGTTTTAATTCTTGGAGAGGTTTCCCTAATTAGTGAACATGGTAGAACAGGTGGCTTCACGTAAATTTTAGTTACCTAAGAAATTAGGATCTATATCTTATCATTGTAACATTGCACAATGCACTCACTCTTTAACCTCCTGACTGATCTTCTGTTTCTTTCTTTGTCATGATGTATGATTGCATGGATTGGGCTGATTCTTAGAAAATTGGTGATTGTAGACTTATTATCTTAATAGAATTCTTAGTGGAGTGATTCCATGAAGAGACAGTGTTAAGAAATGCGTGTATATTCAAGGCTTTAGTTAGTGGAGAGGAATAAGGATGCATTAACTTGTATAATAAAAATGAAAGAAGAAAGATCAGTCCATGAGAGTTATATATGTTCTGTTGCAAACGAATACTCTACATCTGTCTTTATCTACTTGAATTAAAATTCCTATTTACCCATTACCTGTTGACCGGTTGATATTTTATGGTAAAATCGATCTCATTGGGTACTTAACCTTATGGGTTACCAAATGGTATTGATATGTACGAAATCCGACATGTTTCTTGTGTCATAGTCTGATGTTGAAATTTTTAAAGCTCTGTTTGACAACTATTTATTTTTTAAAAATTAAGCCTATAAACATGATTTTCATTTATTACTTATTAGTTTATATATTTTCTAGTTTTCATTTTATGAGTGTTCAAAATCAAAGCCAAAAATTAGAGGAAAAAAAGATTTTCAAAAACTTATTTTTGTTTTCGGAAATTGGCCAAATATTCAAATGTTTAGATAGGAAATATGAAAACCACGAGTAAAAAATGGTTCAAGTTTCTTTTTCTTGATTACGTTTTCTTTACCATAGATCTCTTGTGTTGTCTTAAATTTGAATGTTGTTTCTATCTTATCTAGATCGAGTGCACACCGATGCCCGAGGAGTACAGCCACGAGGTAGTTGCTTCTATACATGTTTCAATTACCACAAGCTAATTTGCTGTCTTCCAACACACTGCATGTCAGAATTCTTCAAACTTAACCGACTACTGTGTTAAACAGGTATCAGTCCACTGCAACGATTGCAGCCATATTAGCAAAGTTCGTTTTCACATTTTGGGACACAAATGCAGTCAGTGCAATTCATTCAATACTTGGAGGACTTCTTCCGGAGGACGTCAATGAAAACTAA

mRNA sequence

ATGGCTGCCATTAGACCTCTAGTTTCCATAACTTTCTTGGCTCTTTTCTTCTTCTCCGAGTCCCTACTCACTTCCCATTCTCTTACCGGAATTCCTCACCGGAACATCTTGAACACAGCTGCGCATCGGATTATGGATGGCAGTGTTGAGTACTCCTCCTACCAAATTAGGAAGTCGAATAAGCTTCATATCAAATATAGAAGAAAGCGGCGGAGTCGACCAATACTAGTCAGCAGCAGCAGCGGCAACCATCATAAATCGGCAGCGAATCGGAGCCGAGTGGCGTCCCTTGAAGGAGACGATCTCCAATGGAAGAAGAACACTTCCCTCTGCCCCAATCCGGCCTCAAACAAGATTTCGGAAAATTGCAATATGGATGCGAGCATTACAGGAGACGATGCAAAATCCGAGCTCCATGCTGCGACCGAATCTTCACCTGTCGCCACTGCCACAACGAGGCCATGGTCTTTTCCTCCCCTCTCTCTATGCCTTTTTCCTTGCACCTCGCTGAGCAATCCCAAGGACCGCCATGAACTTGTTCGCCAGGATATTAGACAAGTGGTTTGCTTGATTTGCAACACAGAACAAGAGGTTGCTAAGGTTTGTCGCAAGTGTAATGTTAATATGGGAGAATACTTTTGCGACATCTGCAAATTCTATGATGATAATACCGAGAAGAAACAGTTTCATTGTGATGCGTGTGGTATCTGTAGGGTTGGTGGTCGTGAAAACTTCTTTCATTGTGAAAGATGTGGATCTTGCTATTCTACTCCTTTGCGTGATAACCACAAATGTGTTGAGAACTCAATGAAAAGTTATTGCCCCATCTGTTGGGAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCCTTGTGGTCACACTATACATTGGGAGTGCTTCAGTGAGATGGAATCAATGAATCAATACCGTTGTCCAATCTGCTTGAAAACAGTTGTGGACATGTCTGCGAGCTGGGCATTGCTGAAAATGGAGATCTCTTGTGTTGTCTTAAATTTGAATGTTGTTTCTATCTTATCTAGATCGAGTGCACACCGATGCCCGAGGAGTACAGCCACGAGGTATCAGTCCACTGCAACGATTGCAGCCATATTAGCAAAGTTCGTTTTCACATTTTGGGACACAAATGCAGTCAGTGCAATTCATTCAATACTTGGAGGACTTCTTCCGGAGGACGTCAATGAAAACTAA

Coding sequence (CDS)

ATGGCTGCCATTAGACCTCTAGTTTCCATAACTTTCTTGGCTCTTTTCTTCTTCTCCGAGTCCCTACTCACTTCCCATTCTCTTACCGGAATTCCTCACCGGAACATCTTGAACACAGCTGCGCATCGGATTATGGATGGCAGTGTTGAGTACTCCTCCTACCAAATTAGGAAGTCGAATAAGCTTCATATCAAATATAGAAGAAAGCGGCGGAGTCGACCAATACTAGTCAGCAGCAGCAGCGGCAACCATCATAAATCGGCAGCGAATCGGAGCCGAGTGGCGTCCCTTGAAGGAGACGATCTCCAATGGAAGAAGAACACTTCCCTCTGCCCCAATCCGGCCTCAAACAAGATTTCGGAAAATTGCAATATGGATGCGAGCATTACAGGAGACGATGCAAAATCCGAGCTCCATGCTGCGACCGAATCTTCACCTGTCGCCACTGCCACAACGAGGCCATGGTCTTTTCCTCCCCTCTCTCTATGCCTTTTTCCTTGCACCTCGCTGAGCAATCCCAAGGACCGCCATGAACTTGTTCGCCAGGATATTAGACAAGTGGTTTGCTTGATTTGCAACACAGAACAAGAGGTTGCTAAGGTTTGTCGCAAGTGTAATGTTAATATGGGAGAATACTTTTGCGACATCTGCAAATTCTATGATGATAATACCGAGAAGAAACAGTTTCATTGTGATGCGTGTGGTATCTGTAGGGTTGGTGGTCGTGAAAACTTCTTTCATTGTGAAAGATGTGGATCTTGCTATTCTACTCCTTTGCGTGATAACCACAAATGTGTTGAGAACTCAATGAAAAGTTATTGCCCCATCTGTTGGGAGTTTCTTTTTGACTCAATAAAAGACACAACTGTTATGCCTTGTGGTCACACTATACATTGGGAGTGCTTCAGTGAGATGGAATCAATGAATCAATACCGTTGTCCAATCTGCTTGAAAACAGTTGTGGACATGTCTGCGAGCTGGGCATTGCTGAAAATGGAGATCTCTTGTGTTGTCTTAAATTTGAATGTTGTTTCTATCTTATCTAGATCGAGTGCACACCGATGCCCGAGGAGTACAGCCACGAGGTATCAGTCCACTGCAACGATTGCAGCCATATTAGCAAAGTTCGTTTTCACATTTTGGGACACAAATGCAGTCAGTGCAATTCATTCAATACTTGGAGGACTTCTTCCGGAGGACGTCAATGAAAACTAA

Protein sequence

MAAIRPLVSITFLALFFFSESLLTSHSLTGIPHRNILNTAAHRIMDGSVEYSSYQIRKSNKLHIKYRRKRRSRPILVSSSSGNHHKSAANRSRVASLEGDDLQWKKNTSLCPNPASNKISENCNMDASITGDDAKSELHAATESSPVATATTRPWSFPPLSLCLFPCTSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKDTTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISCVVLNLNVVSILSRSSAHRCPRSTATRYQSTATIAAILAKFVFTFWDTNAVSAIHSILGGLLPEDVNEN*
Homology
BLAST of Chy1G016420 vs. ExPASy Swiss-Prot
Match: Q8VZK0 (E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 6.9e-69
Identity = 108/172 (62.79%), Postives = 140/172 (81.40%), Query Frame = 0

Query: 168 TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 227
           ++L N  DRH+LVRQD++QV+C +C+TEQ  A+VC  C VNMGEYFC IC FYDD+TEK+
Sbjct: 47  STLRNIYDRHDLVRQDVKQVICSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQ 106

Query: 228 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 287
           QFHCD CGICRVGGRENFFHC++CGSCY+  LR+NH+CVENSM+ +CPIC+E+LFDS+KD
Sbjct: 107 QFHCDDCGICRVGGRENFFHCKKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKD 166

Query: 288 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISCVVL 340
           T VM CGHT+H EC++EM   +++ CPIC ++V+DMS +W  L  EI    +
Sbjct: 167 TNVMKCGHTMHVECYNEMIKRDKFCCPICSRSVIDMSKTWQRLDEEIEATAM 218

BLAST of Chy1G016420 vs. ExPASy Swiss-Prot
Match: Q10LI1 (E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SRFP1 PE=1 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 3.2e-58
Identity = 99/208 (47.60%), Postives = 136/208 (65.38%), Query Frame = 0

Query: 164 LFPCTSLSN--PKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYD 223
           +F C    N   KD HEL R  +  V+CL+C+TEQ VA+VC  C V MGEYFC  CKF+D
Sbjct: 35  VFHCRHCHNESTKDGHELDRHAVESVICLVCDTEQPVAQVCYNCGVCMGEYFCSACKFFD 94

Query: 224 DNTEKKQFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFL 283
           D+ +++ FHC  CGICRVGG++NFFHCE+CGSCYS  LRD H C+ENSMK+ CPIC+E+L
Sbjct: 95  DDVDREHFHCQDCGICRVGGKDNFFHCEKCGSCYSVSLRDKHCCIENSMKNNCPICYEYL 154

Query: 284 FDSIKDTTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISCVVLNL 343
           FDS+++T+V+ CGHT+H +CF EM   +++ CPIC   + DM      L  EI   +L++
Sbjct: 155 FDSLRETSVLRCGHTMHLQCFHEMLKHDKFSCPICSMPIFDMDKFLRALDAEIEANMLHI 214

Query: 344 --------------NVVSILSRSSAHRC 356
                         +   + +R + H+C
Sbjct: 215 DYMGKGWIVCNDCRDTTQVYARVAGHKC 242

BLAST of Chy1G016420 vs. ExPASy Swiss-Prot
Match: Q5JL96 (Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=39947 GN=RZFP34 PE=2 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 6.1e-57
Identity = 88/162 (54.32%), Postives = 122/162 (75.31%), Query Frame = 0

Query: 176 RHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDACG 235
           RHEL R +++QV+C +C TEQEV +VC  C V MG+YFC++CK +DD+  K+Q+HC+ CG
Sbjct: 99  RHELPRHEVQQVICSLCGTEQEVRQVCISCGVCMGKYFCEVCKLFDDDVSKQQYHCNGCG 158

Query: 236 ICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKDTTVMPCGH 295
           ICR+GG+ENFFHC +CG CYS  L+++H CVE +M   CPIC+E+LF+S  D +V+PCGH
Sbjct: 159 ICRIGGKENFFHCSKCGCCYSIVLKNSHACVEGAMHHDCPICFEYLFESTNDVSVLPCGH 218

Query: 296 TIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISCV 338
           TIH +C  EME   Q+ CP+C K+V DMS +W  L  E++ +
Sbjct: 219 TIHVKCLREMEEHCQFACPLCSKSVCDMSKAWERLDEELATI 260

BLAST of Chy1G016420 vs. ExPASy Swiss-Prot
Match: Q9FFB6 (E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=1 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 2.8e-54
Identity = 86/160 (53.75%), Postives = 114/160 (71.25%), Query Frame = 0

Query: 176 RHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDACG 235
           RHEL R ++ +V+C +C TEQ+V + C  C V MG+YFC  CKF+DD+  KKQ+HCD CG
Sbjct: 65  RHELPRHEVSKVICSLCETEQDVQQNCSNCGVCMGKYFCSKCKFFDDDLSKKQYHCDECG 124

Query: 236 ICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKDTTVMPCGH 295
           ICR GG ENFFHC+RC  CYS  + D H+CVE +M   CP+C+E+LFDS +D TV+ CGH
Sbjct: 125 ICRTGGEENFFHCKRCRCCYSKIMEDKHQCVEGAMHHNCPVCFEYLFDSTRDITVLRCGH 184

Query: 296 TIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIS 336
           T+H EC  +M   N+Y CP+C K++ DMS  W  L  E++
Sbjct: 185 TMHLECTKDMGLHNRYTCPVCSKSICDMSNLWKKLDEEVA 224

BLAST of Chy1G016420 vs. ExPASy Swiss-Prot
Match: Q9CR50 (RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=10090 GN=Rchy1 PE=1 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 2.6e-39
Identity = 68/172 (39.53%), Postives = 103/172 (59.88%), Query Frame = 0

Query: 164 LFPCTSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDN 223
           L+ C    +  + H+L R  +++V C+ C   Q   + C  C+   GEY+C IC  +D  
Sbjct: 37  LYTCRLCHDTNEDHQLDRFKVKEVQCINCEKLQHAQQTCEDCSTLFGEYYCSICHLFD-- 96

Query: 224 TEKKQFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFD 283
            +K+Q+HC++CGICR+G +E+FFHC +C  C +T LR  HKC+EN  +  CPIC E +  
Sbjct: 97  KDKRQYHCESCGICRIGPKEDFFHCLKCNLCLTTNLRGKHKCIENVSRQNCPICLEDIHT 156

Query: 284 SIKDTTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIS 336
           S     V+PCGH +H  C+ EM     YRCP+C+ + +DM+  W  L  E++
Sbjct: 157 SRVVAHVLPCGHLLHRTCYEEM-LKEGYRCPLCMHSALDMTRYWRQLDTEVA 205

BLAST of Chy1G016420 vs. ExPASy TrEMBL
Match: A0A1S3BT53 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo OX=3656 GN=LOC103492883 PE=4 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 2.1e-121
Identity = 235/336 (69.94%), Postives = 241/336 (71.73%), Query Frame = 0

Query: 1   MAAIRPLVSITFLALFFFSESLLTSHSLTGIPHRNILNTAAHRIMDGSVEYSSYQIRKSN 60
           MAAIRPLVSI  LAL FFSESLLTSHSLTGIPHRNIL+T AHRI++GSVE SSYQ RKSN
Sbjct: 1   MAAIRPLVSIALLALLFFSESLLTSHSLTGIPHRNILHTVAHRIVNGSVE-SSYQ-RKSN 60

Query: 61  KLHIKYRRKRRSRPILVSSSSGNHHKSAANRSRVASLEGDDLQWKKNTSLCPNPASNKIS 120
           KLH K R  RR RPIL+S S+G HHKSA NRSRVAS E                      
Sbjct: 61  KLHTKDR--RRIRPILISGSNGMHHKSAENRSRVASFE---------------------- 120

Query: 121 ENCNMDASITGDDAKSELHAATESSPVATATTRPWSFPPLSLCLFPCTSLSNPKDRHELV 180
                                                                 DRHELV
Sbjct: 121 ------------------------------------------------------DRHELV 180

Query: 181 RQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDACGICRVG 240
           R+DI QVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDACGICRVG
Sbjct: 181 RRDIGQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDACGICRVG 240

Query: 241 GRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKDTTVMPCGHTIHWE 300
           GRENFFHCERCGSCYSTPLRDNH CVENSMKSYCPICWEFLFDSIKDTTVMPCGHTIH E
Sbjct: 241 GRENFFHCERCGSCYSTPLRDNHTCVENSMKSYCPICWEFLFDSIKDTTVMPCGHTIHLE 256

Query: 301 CFSEMESMNQYRCPICLKTVVDMSASWALLKMEISC 337
           CFSEMESMNQYRCPICLKTVVDMSASWALLKMEI C
Sbjct: 301 CFSEMESMNQYRCPICLKTVVDMSASWALLKMEIEC 256

BLAST of Chy1G016420 vs. ExPASy TrEMBL
Match: A0A0A0KAQ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G394010 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 9.3e-101
Identity = 168/169 (99.41%), Postives = 168/169 (99.41%), Query Frame = 0

Query: 168 TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 227
           TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK
Sbjct: 53  TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 112

Query: 228 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 287
           QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD
Sbjct: 113 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 172

Query: 288 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISC 337
           TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEI C
Sbjct: 173 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIEC 221

BLAST of Chy1G016420 vs. ExPASy TrEMBL
Match: A0A5D3D3Y9 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001940 PE=4 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 1.5e-98
Identity = 166/169 (98.22%), Postives = 166/169 (98.22%), Query Frame = 0

Query: 168 TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 227
           TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK
Sbjct: 52  TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 111

Query: 228 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 287
           QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNH CVENSMKSYCPICWEFLFDSIKD
Sbjct: 112 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHTCVENSMKSYCPICWEFLFDSIKD 171

Query: 288 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISC 337
           TTVMPCGHTIH ECFSEMESMNQYRCPICLKTVVDMSASWALLKMEI C
Sbjct: 172 TTVMPCGHTIHLECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIEC 220

BLAST of Chy1G016420 vs. ExPASy TrEMBL
Match: A0A5A7UVJ3 (E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold497G00010 PE=4 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 1.9e-98
Identity = 170/190 (89.47%), Postives = 174/190 (91.58%), Query Frame = 0

Query: 168 TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 227
           TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK
Sbjct: 52  TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 111

Query: 228 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 287
           QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNH CVENSMKSYCPICWEFLFDSIKD
Sbjct: 112 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHTCVENSMKSYCPICWEFLFDSIKD 171

Query: 288 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISCVVL----NLNV 347
           TTVMPCGHTIH ECFSEMESMNQYRCPICLKTVVDMSASWALLKMEI C  +    +  V
Sbjct: 172 TTVMPCGHTIHLECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIECTPMPEEYSHEV 231

Query: 348 VSILSRSSAH 354
           VS+     +H
Sbjct: 232 VSVHCNDCSH 241

BLAST of Chy1G016420 vs. ExPASy TrEMBL
Match: A0A6J1KQY2 (E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE=4 SV=1)

HSP 1 Score: 345.1 bits (884), Expect = 3.9e-91
Identity = 156/189 (82.54%), Postives = 168/189 (88.89%), Query Frame = 0

Query: 168 TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 227
           +SLSNPKDRHELVRQD+RQVVCLICNTEQE  +VCR CNVNMGEYFCDICKFYDD+T+KK
Sbjct: 53  SSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVCRNCNVNMGEYFCDICKFYDDDTKKK 112

Query: 228 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 287
           QFHCDACGICRVGGRENFFHCE+CGSCYSTPLRDNH CVENSMKS+CPIC+EFLFDSIKD
Sbjct: 113 QFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDNHMCVENSMKSFCPICYEFLFDSIKD 172

Query: 288 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISCVVL---NLNVV 347
           TTVMPCGHTIH ECF EMES NQYRCPIC KTV+DMSASWALLKMEI C  +     + V
Sbjct: 173 TTVMPCGHTIHLECFREMESQNQYRCPICSKTVMDMSASWALLKMEIDCTPMPEEYSHEV 232

Query: 348 SILSRSSAH 354
           SIL    +H
Sbjct: 233 SILCNDCSH 241

BLAST of Chy1G016420 vs. NCBI nr
Match: XP_008451651.1 (PREDICTED: E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo])

HSP 1 Score: 439 bits (1130), Expect = 4.88e-151
Identity = 235/336 (69.94%), Postives = 241/336 (71.73%), Query Frame = 0

Query: 1   MAAIRPLVSITFLALFFFSESLLTSHSLTGIPHRNILNTAAHRIMDGSVEYSSYQIRKSN 60
           MAAIRPLVSI  LAL FFSESLLTSHSLTGIPHRNIL+T AHRI++GSVE SSYQ RKSN
Sbjct: 1   MAAIRPLVSIALLALLFFSESLLTSHSLTGIPHRNILHTVAHRIVNGSVE-SSYQ-RKSN 60

Query: 61  KLHIKYRRKRRSRPILVSSSSGNHHKSAANRSRVASLEGDDLQWKKNTSLCPNPASNKIS 120
           KLH K RR  R RPIL+S S+G HHKSA NRSRVAS E                      
Sbjct: 61  KLHTKDRR--RIRPILISGSNGMHHKSAENRSRVASFE---------------------- 120

Query: 121 ENCNMDASITGDDAKSELHAATESSPVATATTRPWSFPPLSLCLFPCTSLSNPKDRHELV 180
                                                                 DRHELV
Sbjct: 121 ------------------------------------------------------DRHELV 180

Query: 181 RQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDACGICRVG 240
           R+DI QVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDACGICRVG
Sbjct: 181 RRDIGQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDACGICRVG 240

Query: 241 GRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKDTTVMPCGHTIHWE 300
           GRENFFHCERCGSCYSTPLRDNH CVENSMKSYCPICWEFLFDSIKDTTVMPCGHTIH E
Sbjct: 241 GRENFFHCERCGSCYSTPLRDNHTCVENSMKSYCPICWEFLFDSIKDTTVMPCGHTIHLE 256

Query: 301 CFSEMESMNQYRCPICLKTVVDMSASWALLKMEISC 336
           CFSEMESMNQYRCPICLKTVVDMSASWALLKMEI C
Sbjct: 301 CFSEMESMNQYRCPICLKTVVDMSASWALLKMEIEC 256

BLAST of Chy1G016420 vs. NCBI nr
Match: KAG7025561.1 (E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392 bits (1008), Expect = 3.81e-131
Identity = 223/394 (56.60%), Postives = 258/394 (65.48%), Query Frame = 0

Query: 1   MAAIRPLVSITFLALFFFSESLLTSH--------SLTGIPHRNILNTAA--HRIMDGSVE 60
           MAAI+ L+SI  +AL  F ESL TS         SL GI  RNI+ T A  H++ +    
Sbjct: 1   MAAIKALLSIALIALLLFFESLPTSAAVHRGASTSLAGILRRNIVQTTADHHQVAE---- 60

Query: 61  YSSYQIRKSNKLHIKYRRKRRSRPILVS---SSSGNHHKSAANRSRVASLEGDDLQWKKN 120
                  K   +HIK  R+ R RP+L+    +S  N  + A N    + +E DD    ++
Sbjct: 61  -------KKFNVHIK--RRMRMRPVLIGRPKTSKANRSREAENEQGWSPMEQDDCPVPQS 120

Query: 121 ------------------------TSLCPNPASNKISE-NCNMDASITGDDAKSELHAAT 180
                                   +S C +  S+ ++     +DASI GDDA+SE  AAT
Sbjct: 121 AGREDFGKLQHGYRFLSFMRRPWPSSSCSSSFSSSVTSFRFELDASIIGDDAESEPRAAT 180

Query: 181 ESSPVATATTRPWSFPPLSLCLFPCTSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVC 240
           ES PVA ATTRP              SLSNPKDRHELVRQD+RQVVCLICNTEQE  +VC
Sbjct: 181 ESLPVAIATTRP-------------CSLSNPKDRHELVRQDVRQVVCLICNTEQEAVRVC 240

Query: 241 RKCNVNMGEYFCDICKFYDDNTEKKQFHCDACGICRVGGRENFFHCERCGSCYSTPLRDN 300
           R CNVNMGEYFCDICKFYDD+T+KKQFHCDACGICRVGGRENFFHCE+CGSCYSTPLRDN
Sbjct: 241 RNCNVNMGEYFCDICKFYDDDTKKKQFHCDACGICRVGGRENFFHCEKCGSCYSTPLRDN 300

Query: 301 HKCVENSMKSYCPICWEFLFDSIKDTTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVD 353
           H CVENSMKS+CPIC+EFLFDSIKDTTVMPCGHTIH ECF EMES NQYRCPIC KTV+D
Sbjct: 301 HMCVENSMKSFCPICYEFLFDSIKDTTVMPCGHTIHLECFREMESQNQYRCPICSKTVMD 360

BLAST of Chy1G016420 vs. NCBI nr
Match: XP_004136056.1 (E3 ubiquitin-protein ligase MIEL1 [Cucumis sativus] >XP_031744839.1 E3 ubiquitin-protein ligase MIEL1 [Cucumis sativus] >KGN44881.1 hypothetical protein Csa_015948 [Cucumis sativus])

HSP 1 Score: 373 bits (957), Expect = 2.59e-125
Identity = 168/169 (99.41%), Postives = 168/169 (99.41%), Query Frame = 0

Query: 168 TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 227
           TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK
Sbjct: 53  TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 112

Query: 228 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 287
           QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD
Sbjct: 113 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 172

Query: 288 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISC 336
           TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEI C
Sbjct: 173 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIEC 221

BLAST of Chy1G016420 vs. NCBI nr
Match: TYK18253.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 365 bits (938), Expect = 1.90e-122
Identity = 166/169 (98.22%), Postives = 166/169 (98.22%), Query Frame = 0

Query: 168 TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 227
           TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK
Sbjct: 52  TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 111

Query: 228 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 287
           QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNH CVENSMKSYCPICWEFLFDSIKD
Sbjct: 112 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHTCVENSMKSYCPICWEFLFDSIKD 171

Query: 288 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISC 336
           TTVMPCGHTIH ECFSEMESMNQYRCPICLKTVVDMSASWALLKMEI C
Sbjct: 172 TTVMPCGHTIHLECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIEC 220

BLAST of Chy1G016420 vs. NCBI nr
Match: KAA0057531.1 (E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa])

HSP 1 Score: 365 bits (938), Expect = 1.97e-122
Identity = 166/169 (98.22%), Postives = 166/169 (98.22%), Query Frame = 0

Query: 168 TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 227
           TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK
Sbjct: 52  TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 111

Query: 228 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 287
           QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNH CVENSMKSYCPICWEFLFDSIKD
Sbjct: 112 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHTCVENSMKSYCPICWEFLFDSIKD 171

Query: 288 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISC 336
           TTVMPCGHTIH ECFSEMESMNQYRCPICLKTVVDMSASWALLKMEI C
Sbjct: 172 TTVMPCGHTIHLECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIEC 220

BLAST of Chy1G016420 vs. TAIR 10
Match: AT3G62970.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 268.5 bits (685), Expect = 8.9e-72
Identity = 112/167 (67.07%), Postives = 136/167 (81.44%), Query Frame = 0

Query: 169 SLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQ 228
           SL +PK+RH+LVRQ+++QVVC IC TEQEVAKVC  C VNMGEYFCDICKF+DD+  K+Q
Sbjct: 65  SLPDPKERHDLVRQNVKQVVCSICQTEQEVAKVCSNCGVNMGEYFCDICKFFDDDISKEQ 124

Query: 229 FHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKDT 288
           FHCD CGICRVGGR+ FFHC+ CG+CY   LRD H C+ENS K+ CP+C+E+LFDS+K  
Sbjct: 125 FHCDDCGICRVGGRDKFFHCQNCGACYGMGLRDKHSCIENSTKNSCPVCYEYLFDSVKAA 184

Query: 289 TVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIS 336
            VM CGHT+H +CF +M + NQYRCPIC K++VDMS SW LL  EIS
Sbjct: 185 HVMKCGHTMHMDCFEQMINENQYRCPICAKSMVDMSPSWHLLDFEIS 231

BLAST of Chy1G016420 vs. TAIR 10
Match: AT5G18650.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 262.7 bits (670), Expect = 4.9e-70
Identity = 108/172 (62.79%), Postives = 140/172 (81.40%), Query Frame = 0

Query: 168 TSLSNPKDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKK 227
           ++L N  DRH+LVRQD++QV+C +C+TEQ  A+VC  C VNMGEYFC IC FYDD+TEK+
Sbjct: 47  STLRNIYDRHDLVRQDVKQVICSVCDTEQPAAQVCSNCGVNMGEYFCSICIFYDDDTEKQ 106

Query: 228 QFHCDACGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKD 287
           QFHCD CGICRVGGRENFFHC++CGSCY+  LR+NH+CVENSM+ +CPIC+E+LFDS+KD
Sbjct: 107 QFHCDDCGICRVGGRENFFHCKKCGSCYAVGLRNNHRCVENSMRHHCPICYEYLFDSLKD 166

Query: 288 TTVMPCGHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISCVVL 340
           T VM CGHT+H EC++EM   +++ CPIC ++V+DMS +W  L  EI    +
Sbjct: 167 TNVMKCGHTMHVECYNEMIKRDKFCCPICSRSVIDMSKTWQRLDEEIEATAM 218

BLAST of Chy1G016420 vs. TAIR 10
Match: AT5G25560.2 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 232.3 bits (591), Expect = 7.1e-61
Identity = 95/162 (58.64%), Postives = 118/162 (72.84%), Query Frame = 0

Query: 174 KDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDA 233
           K RH++ R  + QV+CL+C TEQEV ++C  C V MG+YFC +CK YDD+T KKQ+HCD 
Sbjct: 99  KQRHDIPRHQVEQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDG 158

Query: 234 CGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKDTTVMPC 293
           CGICR+GGRENFFHC +CG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PC
Sbjct: 159 CGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPC 218

Query: 294 GHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEIS 336
           GHTIH +C  EM    QY CP+C K+V DMS  W    MEI+
Sbjct: 219 GHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIA 260

BLAST of Chy1G016420 vs. TAIR 10
Match: AT5G25560.1 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 230.7 bits (587), Expect = 2.1e-60
Identity = 98/194 (50.52%), Postives = 128/194 (65.98%), Query Frame = 0

Query: 174 KDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDA 233
           K RH++ R  + QV+CL+C TEQEV ++C  C V MG+YFC +CK YDD+T KKQ+HCD 
Sbjct: 99  KQRHDIPRHQVEQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDG 158

Query: 234 CGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKDTTVMPC 293
           CGICR+GGRENFFHC +CG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PC
Sbjct: 159 CGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPC 218

Query: 294 GHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISCVVLNLNVVSILSRSSAH 353
           GHTIH +C  EM    QY CP+C K+V DMS  W    MEI+   +     + + +   +
Sbjct: 219 GHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCN 278

Query: 354 RCPRSTATRYQSTA 368
            C +    +Y   A
Sbjct: 279 DCGKKAEVQYHVVA 292

BLAST of Chy1G016420 vs. TAIR 10
Match: AT5G25560.4 (CHY-type/CTCHY-type/RING-type Zinc finger protein )

HSP 1 Score: 230.7 bits (587), Expect = 2.1e-60
Identity = 98/194 (50.52%), Postives = 128/194 (65.98%), Query Frame = 0

Query: 174 KDRHELVRQDIRQVVCLICNTEQEVAKVCRKCNVNMGEYFCDICKFYDDNTEKKQFHCDA 233
           K RH++ R  + QV+CL+C TEQEV ++C  C V MG+YFC +CK YDD+T KKQ+HCD 
Sbjct: 94  KQRHDIPRHQVEQVICLLCGTEQEVGQICIHCGVCMGKYFCKVCKLYDDDTSKKQYHCDG 153

Query: 234 CGICRVGGRENFFHCERCGSCYSTPLRDNHKCVENSMKSYCPICWEFLFDSIKDTTVMPC 293
           CGICR+GGRENFFHC +CG CYS  L++ H CVE +M   CPIC+EFLF+S  D TV+PC
Sbjct: 154 CGICRIGGRENFFHCYKCGCCYSILLKNGHPCVEGAMHHDCPICFEFLFESRNDVTVLPC 213

Query: 294 GHTIHWECFSEMESMNQYRCPICLKTVVDMSASWALLKMEISCVVLNLNVVSILSRSSAH 353
           GHTIH +C  EM    QY CP+C K+V DMS  W    MEI+   +     + + +   +
Sbjct: 214 GHTIHQKCLEEMRDHYQYACPLCSKSVCDMSKVWEKFDMEIAATPMPEPYQNRMVQILCN 273

Query: 354 RCPRSTATRYQSTA 368
            C +    +Y   A
Sbjct: 274 DCGKKAEVQYHVVA 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8VZK06.9e-6962.79E3 ubiquitin-protein ligase MIEL1 OS=Arabidopsis thaliana OX=3702 GN=MIEL1 PE=1 ... [more]
Q10LI13.2e-5847.60E3 ubiquitin-protein ligase SRFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SR... [more]
Q5JL966.1e-5754.32Probable E3 ubiquitin-protein ligase RZFP34 OS=Oryza sativa subsp. japonica OX=3... [more]
Q9FFB62.8e-5453.75E3 ubiquitin-protein ligase RZFP34 OS=Arabidopsis thaliana OX=3702 GN=RZPF34 PE=... [more]
Q9CR502.6e-3939.53RING finger and CHY zinc finger domain-containing protein 1 OS=Mus musculus OX=1... [more]
Match NameE-valueIdentityDescription
A0A1S3BT532.1e-12169.94E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo OX=3656 GN=LOC103492883 P... [more]
A0A0A0KAQ19.3e-10199.41Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G394010 PE=4 SV=1[more]
A0A5D3D3Y91.5e-9898.22E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A5A7UVJ31.9e-9889.47E3 ubiquitin-protein ligase MIEL1-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A6J1KQY23.9e-9182.54E3 ubiquitin-protein ligase MIEL1 OS=Cucurbita maxima OX=3661 GN=LOC111497863 PE... [more]
Match NameE-valueIdentityDescription
XP_008451651.14.88e-15169.94PREDICTED: E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo][more]
KAG7025561.13.81e-13156.60E3 ubiquitin-protein ligase MIEL1, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_004136056.12.59e-12599.41E3 ubiquitin-protein ligase MIEL1 [Cucumis sativus] >XP_031744839.1 E3 ubiquitin... [more]
TYK18253.11.90e-12298.22E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa][more]
KAA0057531.11.97e-12298.22E3 ubiquitin-protein ligase MIEL1-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
AT3G62970.18.9e-7267.07zinc finger (C3HC4-type RING finger) family protein [more]
AT5G18650.14.9e-7062.79CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.27.1e-6158.64CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.12.1e-6050.52CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
AT5G25560.42.1e-6050.52CHY-type/CTCHY-type/RING-type Zinc finger protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 274..316
e-value: 1.2E-7
score: 41.4
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 273..316
e-value: 2.0E-8
score: 34.4
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 274..316
score: 11.633604
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 260..337
e-value: 7.9E-10
score: 39.8
IPR008913Zinc finger, CHY-typePFAMPF05495zf-CHYcoord: 164..219
e-value: 1.5E-10
score: 41.4
IPR008913Zinc finger, CHY-typePROSITEPS51266ZF_CHYcoord: 139..207
score: 10.493858
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..97
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..90
NoneNo IPR availablePANTHERPTHR21319RING FINGER AND CHY ZINC FINGER DOMAIN-CONTAINING PROTEIN 1coord: 171..342
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 273..323
IPR031109Ring finger and CHY zinc finger domain-containing protein, plantPANTHERPTHR21319:SF12ZINC FINGER (C3HC4-TYPE RING FINGER) FAMILY PROTEINcoord: 171..342
IPR017921Zinc finger, CTCHY-typePROSITEPS51270ZF_CTCHYcoord: 209..273
score: 32.965549
IPR037274Zinc finger, CHY-type superfamilySUPERFAMILY161219CHY zinc finger-likecoord: 164..208
IPR037275Zinc finger, CTCHY-type superfamilySUPERFAMILY161245Zinc hairpin stackcoord: 210..267

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G016420.1Chy1G016420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding