Chy1G015130 (gene) Cucumber (hystrix) v1

Overview
NameChy1G015130
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionprotein KINESIN LIGHT CHAIN-RELATED 1
LocationchrH01: 20845800 .. 20848672 (+)
RNA-Seq ExpressionChy1G015130
SyntenyChy1G015130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAGAAATGCTTCAATTTCCCTTCTTCCTTACTTCAATCGTCTCAATCTCCGCTCACTTTCATCGCTCATTTCCCCAAATTCGATTTCTAATCTCCCTTCAAATTCCTACCCTGTTTCAACTCCCAATCTCAAGCCCTGCACCAAAAACAATGGACTGTTCTTCAAGTTCACTCGATTTCAAGCAAACCCATCTCGAAACTTGGATACCCTCGTGGAGAAACGGCCTGAAATTTCCTCAAGACAGCGAAAACTCAAGGACAAATCCGTGCTTGAGGAGTCTTTTGAGTCTGCTGACACTGTTGATGAAATGTTCAAGGCCTTTAAAGAAATGGAATCCGCTTTTGAAGAGAAGGATCTCGGGTTGGCTTCCTTGAAAATTGCTCTGAAACTTGACCAAGAAGGTGAGGATCCTGAACAAATTTTGTTGTATGCTGATAGAGCTTTAAAAGCTTTGGATAAAGATGATAATAGGAATCCTTCTTTACCTGTTGCTATGACTTTACAATTGATTGGTTCTGTTAATTATAGTTTGAAGAGGTTTAGTGACAGTTTGGGGTACTTGAATAGGGCTAATAGGATTCTGGGTCAGTTAGAGGAGAAGGGTTTTAGTGCTGAGGATATTAGGCCAGTACTTCATGCTGTGCTACTTGAGTTGGCAAATGTTAAAACTGCAATGGGGAGAAGAGAAGAAGCGCTCTCTAATCTCAGGAAGTGTTTAGAGATTAAGGAGTTGATGTTGGAAAAGGATAGTAGAGAATTAGGGACGGCGAATCGTGATTTGGCCGAAGCTTATGTTGCTATATTGAACTTTAAGGATGCTCTTCCCTTTTGTATGAAGGGATTGGAGATACATAAGAAGGAGTTGGGCAATAATTCAGTTGAAGTTGCTCATGATAGGAGGCTTCTTGGGGTGATTTATAGTGGGTTGGAAGAATATCAAAAGGCATTGGAGCAGAATGAATTGTCTCGGAAGGTTTTGAAGAACTGGGGCCTTAATTCTGATTTGCTTCGTGCAGAAACCGATGCTGCTAATATGCAAATTGCACTCGGTAGGTACGATGAGGCCATTAATACTCTTAAGGATGTTGCTCAGCATACAGATAAAGACAGTGAACTTCGGGGAGTTGTGTTTATCTCAATGGGAAAAGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAGATTGCTAGTGGAATTTTCGACAAGAGAGAAGGAACTTCTCCTGTGGAAGCTGCTGAGGCGTATTCAGAGATATCAATGCAGTATGAGACAATGAATGAGTTTGACACTGCAATTTCTTTATTAAAAAAATCTCTAGCTTTGCTTGAGAAGCTTCCACAGGAGCAGCACTCGGAGGGTAGTGTTTCAGCGAGATTAGGGTGGTTGCTACTGTTGACTGGTAAGGTTCAAGAAGCTATTCCATACTTGGAGGGTGCAGCAGAGACTTTAAAAGAGTGTTTTGGATCTAATCATTTTGGAGTTGGATATATATACAATAATCTTGGAGCTGCGTATTTGGAACTCGAGAGACCTCAATCGGCTGCACAAATGTTTGCAGTGGCGAAAGACATTATGGATGTATCACTTGGCCCCCATCATGCAGATTCAATTGAAGCCTGTCACAATCTTTCAAAGGCATATGGTACAATGGGAAGGTATGGTTACTTTCAAGCGTCATTTTTTCCTCCATCGCTGGTTACATTCTTTACAACGTACAATGTTTTGCTTATTATTCATTGAAATATCTATTGGGGGAGTTGGGTTTTATTAATGATATTGAGACACAGAACCATATAGATTGAGATCAGAATGTTTTGTTTTGAATGGCAGAGTAGGAAATTGATTCATATAATATGTCTGATGTCAAAACTGTTCATTTCCTTTTGGAGATATATTTAATTTCATCATGTACGATAAATTCACTTGCCATTGTTTGTTCTTAAAACCAAATGTGGCATGTTTTATATACTTGCCCCATTCTGCTTAGGTTAACTGTACTTTTACGGTTGTGGAAGAACTAGTGGTTAATTGGATACACAAAAATGTCAAGAAGACCTTGATGGGACTCTAACCAATTATTGGATGACCCAAACTAATGGGTTCATCTTGGACTGGAATTTCCTTTGAGTCACATGACAGGAATGATAAAGTATCATTAGTATTTTAGAATAGGAATATTACAGGGCAGAGAGCTTTTGCCGGTCTTGTTAAAAAAAGTGAGTATGGTGAATACTGATAAGTCTGTATTTTATAGCTATCGTTATATGGGAATATCTAAGTTGTGTACGGAGATGAATGCTTGTAGACCTAACTTGATACTGCAAGTTCGACCTATGGGCTGAGTTGATTTTGAAATTTGATCACTTTGTACTTTTAACTATCAATCTGTCCTGAATGTATAACACTATGTTCAGTTCAGTCCTGCATCTTTCTTATTGCCCTTGGTATCTTAATCATTTATTATTGAATGTTGCCAAAACTGCAGAAATTAAAACTATTCTTTTCTACCAACATCGTAATGAAAACCTTTGTTTCTGGATCCATGGATCACAGCAGCAATTTGGCTAGTGATGGCCACTCATGAACTCATTACTTTACTAATCTTTTCTATGTGTTGGTGCAGTTATGCCCTTGCAATTGAATTCCAGGAGCAAGTGGTTGATGCTTGGGACAGCCATGGCTCGAGTGCCGAAGACGAACTTAGAGAAGCCCGACGAACGCTAGAACAATTGAAGAGGAAAGCTCAAGGTACATCTAAATTTGAGCACTCAACAAAGGCCTTGCCCCTCCCTCATTCCTCAACTTCTTCCAAAACTCCACAGCCCGATCTCCCTACCCCTCAGAAACATCAATAA

mRNA sequence

ATGAGAAGAAATGCTTCAATTTCCCTTCTTCCTTACTTCAATCGTCTCAATCTCCGCTCACTTTCATCGCTCATTTCCCCAAATTCGATTTCTAATCTCCCTTCAAATTCCTACCCTGTTTCAACTCCCAATCTCAAGCCCTGCACCAAAAACAATGGACTGTTCTTCAAGTTCACTCGATTTCAAGCAAACCCATCTCGAAACTTGGATACCCTCGTGGAGAAACGGCCTGAAATTTCCTCAAGACAGCGAAAACTCAAGGACAAATCCGTGCTTGAGGAGTCTTTTGAGTCTGCTGACACTGTTGATGAAATGTTCAAGGCCTTTAAAGAAATGGAATCCGCTTTTGAAGAGAAGGATCTCGGGTTGGCTTCCTTGAAAATTGCTCTGAAACTTGACCAAGAAGGTGAGGATCCTGAACAAATTTTGTTGTATGCTGATAGAGCTTTAAAAGCTTTGGATAAAGATGATAATAGGAATCCTTCTTTACCTGTTGCTATGACTTTACAATTGATTGGTTCTGTTAATTATAGTTTGAAGAGGTTTAGTGACAGTTTGGGGTACTTGAATAGGGCTAATAGGATTCTGGGTCAGTTAGAGGAGAAGGGTTTTAGTGCTGAGGATATTAGGCCAGTACTTCATGCTGTGCTACTTGAGTTGGCAAATGTTAAAACTGCAATGGGGAGAAGAGAAGAAGCGCTCTCTAATCTCAGGAAGTGTTTAGAGATTAAGGAGTTGATGTTGGAAAAGGATAGTAGAGAATTAGGGACGGCGAATCGTGATTTGGCCGAAGCTTATGTTGCTATATTGAACTTTAAGGATGCTCTTCCCTTTTGTATGAAGGGATTGGAGATACATAAGAAGGAGTTGGGCAATAATTCAGTTGAAGTTGCTCATGATAGGAGGCTTCTTGGGGTGATTTATAGTGGGTTGGAAGAATATCAAAAGGCATTGGAGCAGAATGAATTGTCTCGGAAGGTTTTGAAGAACTGGGGCCTTAATTCTGATTTGCTTCGTGCAGAAACCGATGCTGCTAATATGCAAATTGCACTCGGTAGGTACGATGAGGCCATTAATACTCTTAAGGATGTTGCTCAGCATACAGATAAAGACAGTGAACTTCGGGGAGTTGTGTTTATCTCAATGGGAAAAGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAGATTGCTAGTGGAATTTTCGACAAGAGAGAAGGAACTTCTCCTGTGGAAGCTGCTGAGGCGTATTCAGAGATATCAATGCAGTATGAGACAATGAATGAGTTTGACACTGCAATTTCTTTATTAAAAAAATCTCTAGCTTTGCTTGAGAAGCTTCCACAGGAGCAGCACTCGGAGGGTAGTGTTTCAGCGAGATTAGGGTGGTTGCTACTGTTGACTGGTAAGGTTCAAGAAGCTATTCCATACTTGGAGGGTGCAGCAGAGACTTTAAAAGAGTGTTTTGGATCTAATCATTTTGGAGTTGGATATATATACAATAATCTTGGAGCTGCGTATTTGGAACTCGAGAGACCTCAATCGGCTGCACAAATGTTTGCAGTGGCGAAAGACATTATGGATGTATCACTTGGCCCCCATCATGCAGATTCAATTGAAGCCTGTCACAATCTTTCAAAGGCATATGGTACAATGGGAAGTTATGCCCTTGCAATTGAATTCCAGGAGCAAGTGGTTGATGCTTGGGACAGCCATGGCTCGAGTGCCGAAGACGAACTTAGAGAAGCCCGACGAACGCTAGAACAATTGAAGAGGAAAGCTCAAGGTACATCTAAATTTGAGCACTCAACAAAGGCCTTGCCCCTCCCTCATTCCTCAACTTCTTCCAAAACTCCACAGCCCGATCTCCCTACCCCTCAGAAACATCAATAA

Coding sequence (CDS)

ATGAGAAGAAATGCTTCAATTTCCCTTCTTCCTTACTTCAATCGTCTCAATCTCCGCTCACTTTCATCGCTCATTTCCCCAAATTCGATTTCTAATCTCCCTTCAAATTCCTACCCTGTTTCAACTCCCAATCTCAAGCCCTGCACCAAAAACAATGGACTGTTCTTCAAGTTCACTCGATTTCAAGCAAACCCATCTCGAAACTTGGATACCCTCGTGGAGAAACGGCCTGAAATTTCCTCAAGACAGCGAAAACTCAAGGACAAATCCGTGCTTGAGGAGTCTTTTGAGTCTGCTGACACTGTTGATGAAATGTTCAAGGCCTTTAAAGAAATGGAATCCGCTTTTGAAGAGAAGGATCTCGGGTTGGCTTCCTTGAAAATTGCTCTGAAACTTGACCAAGAAGGTGAGGATCCTGAACAAATTTTGTTGTATGCTGATAGAGCTTTAAAAGCTTTGGATAAAGATGATAATAGGAATCCTTCTTTACCTGTTGCTATGACTTTACAATTGATTGGTTCTGTTAATTATAGTTTGAAGAGGTTTAGTGACAGTTTGGGGTACTTGAATAGGGCTAATAGGATTCTGGGTCAGTTAGAGGAGAAGGGTTTTAGTGCTGAGGATATTAGGCCAGTACTTCATGCTGTGCTACTTGAGTTGGCAAATGTTAAAACTGCAATGGGGAGAAGAGAAGAAGCGCTCTCTAATCTCAGGAAGTGTTTAGAGATTAAGGAGTTGATGTTGGAAAAGGATAGTAGAGAATTAGGGACGGCGAATCGTGATTTGGCCGAAGCTTATGTTGCTATATTGAACTTTAAGGATGCTCTTCCCTTTTGTATGAAGGGATTGGAGATACATAAGAAGGAGTTGGGCAATAATTCAGTTGAAGTTGCTCATGATAGGAGGCTTCTTGGGGTGATTTATAGTGGGTTGGAAGAATATCAAAAGGCATTGGAGCAGAATGAATTGTCTCGGAAGGTTTTGAAGAACTGGGGCCTTAATTCTGATTTGCTTCGTGCAGAAACCGATGCTGCTAATATGCAAATTGCACTCGGTAGGTACGATGAGGCCATTAATACTCTTAAGGATGTTGCTCAGCATACAGATAAAGACAGTGAACTTCGGGGAGTTGTGTTTATCTCAATGGGAAAAGTGTTGTGTAATCAGGAAAAGTTTACTGATGCAAAGAGATGTTTGGAGATTGCTAGTGGAATTTTCGACAAGAGAGAAGGAACTTCTCCTGTGGAAGCTGCTGAGGCGTATTCAGAGATATCAATGCAGTATGAGACAATGAATGAGTTTGACACTGCAATTTCTTTATTAAAAAAATCTCTAGCTTTGCTTGAGAAGCTTCCACAGGAGCAGCACTCGGAGGGTAGTGTTTCAGCGAGATTAGGGTGGTTGCTACTGTTGACTGGTAAGGTTCAAGAAGCTATTCCATACTTGGAGGGTGCAGCAGAGACTTTAAAAGAGTGTTTTGGATCTAATCATTTTGGAGTTGGATATATATACAATAATCTTGGAGCTGCGTATTTGGAACTCGAGAGACCTCAATCGGCTGCACAAATGTTTGCAGTGGCGAAAGACATTATGGATGTATCACTTGGCCCCCATCATGCAGATTCAATTGAAGCCTGTCACAATCTTTCAAAGGCATATGGTACAATGGGAAGTTATGCCCTTGCAATTGAATTCCAGGAGCAAGTGGTTGATGCTTGGGACAGCCATGGCTCGAGTGCCGAAGACGAACTTAGAGAAGCCCGACGAACGCTAGAACAATTGAAGAGGAAAGCTCAAGGTACATCTAAATTTGAGCACTCAACAAAGGCCTTGCCCCTCCCTCATTCCTCAACTTCTTCCAAAACTCCACAGCCCGATCTCCCTACCCCTCAGAAACATCAATAA

Protein sequence

MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSYPVSTPNLKPCTKNNGLFFKFTRFQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEKDLGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLKRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKCLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHDRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINTLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEAYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQGTSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ*
Homology
BLAST of Chy1G015130 vs. ExPASy Swiss-Prot
Match: Q9LII8 (Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 PE=1 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 2.7e-43
Identity = 137/497 (27.57%), Postives = 236/497 (47.48%), Query Frame = 0

Query: 117 EEKDLGLASLKIALKLDQEGEDPEQILLYADRALKALDK--DDNRNPSLPVAMTLQLIGS 176
           E  +LG+  LK A +L   GE+  + L  A RA+K  +K  +  +   L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 177 VNYSLKRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEAL 236
           +   L R++D++  L R+  I   + E G   ED      A  ++L ++   MG+ E ++
Sbjct: 209 IYAGLGRYNDAVPVLERSIEI--PMIEDG---EDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 237 SNLRKCLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKE--LGN 296
                 LEI+  +L +    +G   R LAEA+V  + F++A   C   L+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 297 NSVEVAHDRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALG 356
            S+E A DR+L+G+I     +Y+ ALE   L+   + +     D+   +    +  ++L 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 357 RYDEAI----NTLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDK 416
           R+DEAI      L    Q   +      +V++ +  +     K  D+K   E A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 417 -REGTSPVEAAEAYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGW 476
              GT   E A  + EIS  Y++MNE D A+ LL+++L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 477 LLLLTGKVQEAIPYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVA 536
           +  + G   E+    + A    +          G   N +G A ++      AA +F  A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 537 KDIMDVSLGPHHADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREA 596
           K I++   GP+H D++    NL+  Y  MG    AIE  E VV   +    +A  E+ + 
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 597 RRTLEQLKRKA-QGTSK 604
           ++ L  L ++A +G SK
Sbjct: 629 KQRLAALLKEAGRGRSK 640

BLAST of Chy1G015130 vs. ExPASy Swiss-Prot
Match: O81629 (Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 1.6e-40
Identity = 159/619 (25.69%), Postives = 280/619 (45.23%), Query Frame = 0

Query: 29  SISNLPSNSYPVSTPNLKPCTKNNGLFFKFTRFQANPSRNLDTLVEKRPEISSRQRKLKD 88
           ++  L S   P  TP L+    +       T+  +NP R   T ++K P  SS   + K 
Sbjct: 3   AMPGLVSVKTPSDTPPLRVAVPD-------TQPLSNPPR---TPMKKTP--SSTPSRSKP 62

Query: 89  KSVLEESFESADTVDEMFKAFKEMES-AFEEKDLGLASLKIALKLDQEGEDPEQILLYAD 148
                   + + TV     A  +++  + +  DLG   LK+A      GE P + L YA 
Sbjct: 63  SPNRSTGKKDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAI 122

Query: 149 RALKALDK-----------DDNRNPSLPVAMTLQLIGSVNYSLKRFSDSLGYLNRANRIL 208
           RA K+ ++             +  P L +AM+L ++ ++  SL RF +++  L RA ++ 
Sbjct: 123 RATKSFERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVP 182

Query: 209 GQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKCLEIKELMLEKDSRELG 268
                      D      +  ++L +  + +G+ + +++   + L+I+   L      +G
Sbjct: 183 DPTR-----GPDHSLAAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVG 242

Query: 269 TANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHDRRLLGVIYSGLEEYQK 328
              R LAEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +Y+ 
Sbjct: 243 ETCRYLAEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYEN 302

Query: 329 ALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAI----NTLKDVAQHTDKDS 388
           ALE   L+   +   G  S++   +    N+ ++L R+DEA+      L        +  
Sbjct: 303 ALEHLVLASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETH 362

Query: 389 ELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKR-EGTSPVEAAEAYSEISMQYETM 448
                VF+ + ++     K  ++K   E A  I++K   GT+  E A   +EIS  YE++
Sbjct: 363 PTVASVFVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESV 422

Query: 449 NEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKE 508
           +E + A+ LL+KS+ LLE  P +Q +   + AR+G +    G+ ++A    E A   L+ 
Sbjct: 423 DEPEEALKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRA 482

Query: 509 CFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHADSIEACHNLSK 568
             G      G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+ 
Sbjct: 483 A-GEKSAFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAA 542

Query: 569 AYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQGTSKFEHST--- 624
            Y  MG    AIE  EQV+   +    +A  +  + ++ L +L ++A  +  ++  +   
Sbjct: 543 TYDAMGRIEDAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEAGRSRNYKAKSLQN 602

BLAST of Chy1G015130 vs. ExPASy Swiss-Prot
Match: F4HSX9 (Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 1.1e-39
Identity = 132/492 (26.83%), Postives = 232/492 (47.15%), Query Frame = 0

Query: 113 ESAFEEKDLGLASLKIALKLDQEGEDPEQILLYADRALKALDKD-DNRNPSLPVAMTLQL 172
           E + E  +L    L  A  L   G+   + L    RA K  +   +N  P L   M L +
Sbjct: 138 EPSSENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHV 197

Query: 173 IGSVNYSLKRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRRE 232
             +V+  LK +++++  L R+  I   + E+G   E+      A L++L +    +G+ E
Sbjct: 198 TAAVHCKLKEYNEAIPVLQRSVEI--PVVEEG---EEHALAKFAGLMQLGDTYAMVGQLE 257

Query: 233 EALSNLRKCLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELG 292
            ++S   + L I++ +L ++   +G   R LAEA V  L F +A   C   L IH++   
Sbjct: 258 SSISCYTEGLNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGL 317

Query: 293 NNSVEVAHDRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIAL 352
             S+  A DRRL+G+I     +++ ALE   L+   +   G  S++   +T   +  ++L
Sbjct: 318 PGSIAEAADRRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSL 377

Query: 353 GRYDEAI----NTLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFD 412
            R+DEAI     +L  +     ++    G V+I +  +     K  +AK   E A  I++
Sbjct: 378 SRFDEAICAYQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYE 437

Query: 413 KRE-GTSPVEAAEAYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLG 472
                 SP E A   ++IS+  E+MNE + AI+LL+K+L +    P ++     + A++G
Sbjct: 438 SHNLEISPEEIASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMG 497

Query: 473 WLLLLTGKVQEAIPYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAV 532
            L  + GK  E+    + A   L+          G   N +G A ++L+  + A ++F  
Sbjct: 498 VLYYMMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEE 557

Query: 533 AKDIMDVSLGPHHADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELRE 592
           AK I++   GP+H +++    NL+ AY  +G    AI+    VV   +    +A     +
Sbjct: 558 AKCILEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTED 617

Query: 593 ARRTLEQLKRKA 599
            +R L QL ++A
Sbjct: 618 EKRRLAQLLKEA 624

BLAST of Chy1G015130 vs. ExPASy Swiss-Prot
Match: A0JM23 (Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2)

HSP 1 Score: 94.0 bits (232), Expect = 6.7e-18
Identity = 93/411 (22.63%), Postives = 174/411 (42.34%), Query Frame = 0

Query: 170  QLIGSVNYSL-KRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMG 229
            QL+G    S+   + D+L    R+     + EEK  S  D+   L   L +L       G
Sbjct: 893  QLVGKDKISMASEYFDALKQYERS----CEGEEKMTSLADLYETLGRFLKDL-------G 952

Query: 230  RREEALSNLRKCLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKK 289
               +A++ L++ LEI+E  L+ D   +  +   LA  YV    F +A     + LEI + 
Sbjct: 953  LLSQAVTPLQRSLEIRETALDPDHPSVAQSLHQLAGVYVQSKKFGNAEQLYKQALEISEN 1012

Query: 290  ELGNNSVEVAHDRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQ 349
              G+  + VA +   L V+Y    ++++A +  + S K+ +        +          
Sbjct: 1013 AYGSEHMRVARELDALAVLYQKQNKFEQAEQLRKKSLKIRQKSARRKGSMYGFALLRRRA 1072

Query: 350  IALGRYDEAINTLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDK 409
            + L    E +   KD + +    +EL GV++     +   +   T  KR LE+   +   
Sbjct: 1073 LQL----EELTLGKDTSDNARTLNEL-GVLYYLQNNL---ETAETFLKRSLEMRERVL-- 1132

Query: 410  REGTSPVEAAEAYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSAR-LGW 469
              G    + A++ + ++  Y    ++D A  L +++L +  +     H   + + + L  
Sbjct: 1133 --GADHPDCAQSINNLAALYNEKKQYDKAEELYERALDIRRRALSPDHPSLAYTVKHLAV 1192

Query: 470  LLLLTGKVQEAIPYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVA 529
            L    GK+ +A+P  E A E  ++ FG  H  V     NL   Y ++++   A+ ++  A
Sbjct: 1193 LYKRKGKLDKAVPLYELAVEIRQKSFGPKHPSVATALVNLAVLYCQMKKQAEASPLYERA 1252

Query: 530  KDIMDVSLGPHHADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGS 579
              I + SLG  H    E   NL+      G +  A E  ++ ++  ++  S
Sbjct: 1253 MKIYEDSLGRMHPRVGETLKNLAVLRYEEGDFEKAAELYKRAMEIKETETS 1280

BLAST of Chy1G015130 vs. ExPASy Swiss-Prot
Match: Q6AZT7 (Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 3.3e-17
Identity = 85/380 (22.37%), Postives = 160/380 (42.11%), Query Frame = 0

Query: 200  EEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKCLEIKELMLEKDSRELGTAN 259
            EEK  S  D+   L   L +L       G   +A++ L++ LEI+E  L+ D   +  + 
Sbjct: 909  EEKMTSLADLYETLGRFLKDL-------GLLSQAVTPLQRSLEIRETALDPDHPSVAQSL 968

Query: 260  RDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHDRRLLGVIYSGLEEYQKALE 319
              LA  Y+    F +A     + LEI +   G+  + VA +   L V+Y    ++++A +
Sbjct: 969  HQLAGVYMQSKKFGNAEQLYKQALEISENAYGSEHLRVARELDALAVLYQKQNKFEQAEQ 1028

Query: 320  QNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINTLKDVAQHTDKDSELRGVVF 379
              + S K+ +        +          + L    E +   KD + +    +EL GV++
Sbjct: 1029 LRKKSLKIRQKSARRKGSMYGFALLRRRALQL----EELTLGKDTSDNARTLNEL-GVLY 1088

Query: 380  ISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEAYSEISMQYETMNEFDTAIS 439
                 +   +   T  KR LE+   +     G    + A++ + ++  Y    ++D A  
Sbjct: 1089 YLQNNL---ETAETFLKRSLEMRERVL----GADHPDCAQSINNLAALYNEKKQYDKAEE 1148

Query: 440  LLKKSLALLEKLPQEQHSEGSVSAR-LGWLLLLTGKVQEAIPYLEGAAETLKECFGSNHF 499
            L +++L +  +     H   + + + L  L    GK+ +A+P  E A +  ++ FG  H 
Sbjct: 1149 LYERALDIRRRALSPDHPSLAYTVKHLAVLYKRKGKLDKAVPLYELAVDIRQKSFGPKHP 1208

Query: 500  GVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHADSIEACHNLSKAYGTMGS 559
             V     NL   Y ++++   A  ++  A  I + SLG  H    E   NL+      G 
Sbjct: 1209 SVATALVNLAVLYCQMKKQDDALPLYERAMKIYEDSLGRMHPRVGETLKNLAVLRYEEGD 1268

Query: 560  YALAIEFQEQVVDAWDSHGS 579
            Y  A E  ++ ++  ++  S
Sbjct: 1269 YEKAAELYKRAMEIKETETS 1269

BLAST of Chy1G015130 vs. ExPASy TrEMBL
Match: A0A0A0K8P3 (TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375840 PE=4 SV=1)

HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 620/634 (97.79%), Postives = 626/634 (98.74%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSYPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNS PVSTPNLKPCT NNGLFFKFTR
Sbjct: 12  MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSSPVSTPNLKPCTINNGLFFKFTR 71

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEKD 120
           FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESA+TVDEMFKAFKEMESAFEEKD
Sbjct: 72  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESAETVDEMFKAFKEMESAFEEKD 131

Query: 121 LGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKIALKLD+EGEDPEQ+LLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK
Sbjct: 132 LGLASLKIALKLDREGEDPEQVLLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 191

Query: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 240
           RFSDSLGYLNRANRILGQLEEKG+SAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC
Sbjct: 192 RFSDSLGYLNRANRILGQLEEKGYSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 251

Query: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300
           LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD
Sbjct: 252 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 311

Query: 301 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 360
           RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT
Sbjct: 312 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 371

Query: 361 LKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 420
           LKDVAQHTDKDSELRGVVF SMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA
Sbjct: 372 LKDVAQHTDKDSELRGVVFFSMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 431

Query: 421 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP
Sbjct: 432 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 491

Query: 481 YLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540
           YLEGAAETLKE FGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA
Sbjct: 492 YLEGAAETLKESFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 551

Query: 541 DSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQG 600
           DSIEACHNLSKAYG MGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKA+G
Sbjct: 552 DSIEACHNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKARG 611

Query: 601 TSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 635
           TSKFEHSTKALPLP SSTSSKT QPDLPT Q+HQ
Sbjct: 612 TSKFEHSTKALPLPQSSTSSKTLQPDLPTRQQHQ 645

BLAST of Chy1G015130 vs. ExPASy TrEMBL
Match: A0A1S3BR15 (nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1)

HSP 1 Score: 1135.2 bits (2935), Expect = 0.0e+00
Identity = 590/634 (93.06%), Postives = 608/634 (95.90%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSYPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASIS+LPYFNRLNLRSLSSLISPNSISNLP NS PVSTPNLKPCTKNNGLFFKFTR
Sbjct: 1   MRRNASISILPYFNRLNLRSLSSLISPNSISNLPLNSSPVSTPNLKPCTKNNGLFFKFTR 60

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEKD 120
           FQAN SRNLDTLVEKRPE+SSRQRKLK+KS LEE+FESADTVDEM KAFK MESAFEEKD
Sbjct: 61  FQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEKD 120

Query: 121 LGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKI LKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK
Sbjct: 121 LGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180

Query: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 240
           RFSDSLGYLNRANRILGQLEEKGFSAED+RPVLHAV LELANVKTAMGRREEALSNLRK 
Sbjct: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRKS 240

Query: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300
           LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD
Sbjct: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300

Query: 301 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 360
           RRLLGVIYSGLEE+QKALEQNELSRKVLKNWGLNSDLLRAE +AANMQIALGRYDEAI+T
Sbjct: 301 RRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIST 360

Query: 361 LKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 420
           LKDV QHTDKDSE+RGVVF SMGKVLCNQEKFTD KRCLEIA GIFDKRE TSP  AAEA
Sbjct: 361 LKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAEA 420

Query: 421 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           YSEISM YETMN+F+TAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAIP
Sbjct: 421 YSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAIP 480

Query: 481 YLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540
           YLEGAAETL+E FGS HFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540

Query: 541 DSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQG 600
           DSIEACHNLSKAYG MGSYALAIEFQ+QVVDAW+SHGS+AEDELREAR+TL+QLKRKAQG
Sbjct: 541 DSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQG 600

Query: 601 TSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 635
           TSKFE STKALPLPHSSTSSKT QPDLP  QKHQ
Sbjct: 601 TSKFELSTKALPLPHSSTSSKTLQPDLPAHQKHQ 634

BLAST of Chy1G015130 vs. ExPASy TrEMBL
Match: A0A5A7UWT8 (Nephrocystin-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00740 PE=4 SV=1)

HSP 1 Score: 1134.8 bits (2934), Expect = 0.0e+00
Identity = 590/634 (93.06%), Postives = 608/634 (95.90%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSYPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASIS+LPYFNRLNLRSLSSLISPNSISNLP NS PVSTPNLKPCTKNNGLFFKFTR
Sbjct: 1   MRRNASISVLPYFNRLNLRSLSSLISPNSISNLPLNSSPVSTPNLKPCTKNNGLFFKFTR 60

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEKD 120
           FQAN SRNLDTLVEKRPE+SSRQRKLK+KS LEE+FESADTVDEM KAFK MESAFEEKD
Sbjct: 61  FQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEKD 120

Query: 121 LGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKI LKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK
Sbjct: 121 LGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180

Query: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 240
           RFSDSLGYLNRANRILGQLEEKGFSAED+RPVLHAV LELANVKTAMGRREEALSNLRK 
Sbjct: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRKS 240

Query: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300
           LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD
Sbjct: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300

Query: 301 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 360
           RRLLGVIYSGLEE+QKALEQNELSRKVLKNWGLNSDLLRAE +AANMQIALGRYDEAI+T
Sbjct: 301 RRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIST 360

Query: 361 LKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 420
           LKDV QHTDKDSE+RGVVF SMGKVLCNQEKFTD KRCLEIA GIFDKRE TSP  AAEA
Sbjct: 361 LKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAEA 420

Query: 421 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           YSEISM YETMN+F+TAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAIP
Sbjct: 421 YSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAIP 480

Query: 481 YLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540
           YLEGAAETL+E FGS HFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540

Query: 541 DSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQG 600
           DSIEACHNLSKAYG MGSYALAIEFQ+QVVDAW+SHGS+AEDELREAR+TL+QLKRKAQG
Sbjct: 541 DSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQG 600

Query: 601 TSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 635
           TSKFE STKALPLPHSSTSSKT QPDLP  QKHQ
Sbjct: 601 TSKFELSTKALPLPHSSTSSKTLQPDLPAHQKHQ 634

BLAST of Chy1G015130 vs. ExPASy TrEMBL
Match: A0A6J1GPX7 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456463 PE=4 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 1.0e-300
Identity = 546/635 (85.98%), Postives = 586/635 (92.28%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISN-LPSNSYPVSTPNLKPCTKNNGLFFKFT 60
           MRRNASISLL + NR+NLRSLSSLISP  IS+  PS S PVS P+L PCTKN+GLFFK +
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKLS 60

Query: 61  RFQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEK 120
           RFQANPSRNL+TLVEKRP+ISSRQRK K+KS LEE+FESA+TVDEM KAFK+MES FEE+
Sbjct: 61  RFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 180
           DLGLASLKI LKL+QE EDPE+IL+YA RALKALDKDDN  PSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRK 240
           KRFSDSLGYLNRANRILGQLEE GFSAE IRPVLHAV LELANVKTAMGRREEAL+NLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 300
           CLEIKEL LEKDSRELG ANRDLAEAYVAILNFKDALPFCM+ LEIHKK+LG+NSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEE+QKALEQNELS+KVLKNWGLNSDLLRAE DAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAE 420
           TLKDV QHT+KDSE + VVFISMGKVL NQEKF DAKRCLEIA+ IFDK+E  SPVEAAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLHNQEKFADAKRCLEIATRIFDKKEAASPVEAAE 420

Query: 421 AYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEF+TAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 540
           PYLEGAAETLKE FGS HFGVGYIYNNLGAAYLELER QSAAQMFA+AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERLQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQ 600
           ADSIEAC NL+KAYG++GSYALAIEFQEQVVDAW+ HGS+AEDELREA+R LEQLKRKA 
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 635
           G SK E S KALPLPHSSTSSKT QPD+P+ QKHQ
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKHQ 635

BLAST of Chy1G015130 vs. ExPASy TrEMBL
Match: A0A6J1JWL0 (protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC111488134 PE=4 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 3.2e-297
Identity = 544/636 (85.53%), Postives = 583/636 (91.67%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSY--PVSTPNLKPCTKNNGLFFKF 60
           MRRNASISLL + NR NLRSLSSLISP  IS+ PS SY  PVST +L PCTKN+GLFFKF
Sbjct: 1   MRRNASISLLFHSNRFNLRSLSSLISPKFISD-PSFSYSSPVSTTHLNPCTKNHGLFFKF 60

Query: 61  TRFQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEE 120
            RFQANPSRNL+TLVEKRP+ISSRQRK K+KS LEE+FESA+TVDEM KAFK+MES FEE
Sbjct: 61  KRFQANPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEE 120

Query: 121 KDLGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYS 180
           +DLGLASLKI LKL+QE EDPE+IL+YA RALKALDKDDN   SLPVAMTLQL+GSVNYS
Sbjct: 121 RDLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKLSLPVAMTLQLLGSVNYS 180

Query: 181 LKRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLR 240
           LKRFSDSLGYLNRANRILGQLEE GFSAE IRPVLHAV LELANVKTAMGRREEAL+NLR
Sbjct: 181 LKRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLR 240

Query: 241 KCLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVA 300
           KCLEIKEL LEKDSRELG A RDLAEAYVAILNFKDAL FCM+ LEIHKK+LG+NSVEVA
Sbjct: 241 KCLEIKELTLEKDSRELGKAKRDLAEAYVAILNFKDALLFCMQALEIHKKQLGHNSVEVA 300

Query: 301 HDRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAI 360
           HDRRLLGVIYSGLEE+QKALEQNELS+KVLKNWGLNSDLLRAE DAANMQIALGRYDEAI
Sbjct: 301 HDRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAI 360

Query: 361 NTLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAA 420
           +TLKDV QHT+KDSE + VVFISMGKVL NQEKF DAKRCLEIA+ IFDK+E  SPVEAA
Sbjct: 361 STLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVEAA 420

Query: 421 EAYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEA 480
           EAYSEISMQYE+MNEF+TAISLLK+SLALLEKLPQEQHS GSVSARLGWLLLLTGKV EA
Sbjct: 421 EAYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSVGSVSARLGWLLLLTGKVHEA 480

Query: 481 IPYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPH 540
           IPYLEGAAETLKE FGS HFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPH
Sbjct: 481 IPYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPH 540

Query: 541 HADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKA 600
           HADSIEAC NL+KAYG++GSYALAIEFQEQVVDAW+ HGS+AEDELREA+R LEQLKRKA
Sbjct: 541 HADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSAAEDELREAQRMLEQLKRKA 600

Query: 601 QGTSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 635
            G SK E S KALPLPHSSTSSKT QPD+P+ Q+HQ
Sbjct: 601 HGKSKIELSMKALPLPHSSTSSKTLQPDIPSSQEHQ 635

BLAST of Chy1G015130 vs. NCBI nr
Match: XP_004148956.2 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucumis sativus] >KGN44742.1 hypothetical protein Csa_016654 [Cucumis sativus])

HSP 1 Score: 1189 bits (3077), Expect = 0.0
Identity = 620/634 (97.79%), Postives = 626/634 (98.74%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSYPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNS PVSTPNLKPCT NNGLFFKFTR
Sbjct: 12  MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSSPVSTPNLKPCTINNGLFFKFTR 71

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEKD 120
           FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESA+TVDEMFKAFKEMESAFEEKD
Sbjct: 72  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESAETVDEMFKAFKEMESAFEEKD 131

Query: 121 LGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKIALKLD+EGEDPEQ+LLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK
Sbjct: 132 LGLASLKIALKLDREGEDPEQVLLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 191

Query: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 240
           RFSDSLGYLNRANRILGQLEEKG+SAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC
Sbjct: 192 RFSDSLGYLNRANRILGQLEEKGYSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 251

Query: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300
           LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD
Sbjct: 252 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 311

Query: 301 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 360
           RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT
Sbjct: 312 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 371

Query: 361 LKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 420
           LKDVAQHTDKDSELRGVVF SMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA
Sbjct: 372 LKDVAQHTDKDSELRGVVFFSMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 431

Query: 421 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP
Sbjct: 432 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 491

Query: 481 YLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540
           YLEGAAETLKE FGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA
Sbjct: 492 YLEGAAETLKESFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 551

Query: 541 DSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQG 600
           DSIEACHNLSKAYG MGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKA+G
Sbjct: 552 DSIEACHNLSKAYGAMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKARG 611

Query: 601 TSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 634
           TSKFEHSTKALPLP SSTSSKT QPDLPT Q+HQ
Sbjct: 612 TSKFEHSTKALPLPQSSTSSKTLQPDLPTRQQHQ 645

BLAST of Chy1G015130 vs. NCBI nr
Match: XP_008451256.1 (PREDICTED: nephrocystin-3 [Cucumis melo])

HSP 1 Score: 1131 bits (2926), Expect = 0.0
Identity = 590/634 (93.06%), Postives = 608/634 (95.90%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSYPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASIS+LPYFNRLNLRSLSSLISPNSISNLP NS PVSTPNLKPCTKNNGLFFKFTR
Sbjct: 1   MRRNASISILPYFNRLNLRSLSSLISPNSISNLPLNSSPVSTPNLKPCTKNNGLFFKFTR 60

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEKD 120
           FQAN SRNLDTLVEKRPE+SSRQRKLK+KS LEE+FESADTVDEM KAFK MESAFEEKD
Sbjct: 61  FQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEKD 120

Query: 121 LGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKI LKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK
Sbjct: 121 LGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180

Query: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 240
           RFSDSLGYLNRANRILGQLEEKGFSAED+RPVLHAV LELANVKTAMGRREEALSNLRK 
Sbjct: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRKS 240

Query: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300
           LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD
Sbjct: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300

Query: 301 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 360
           RRLLGVIYSGLEE+QKALEQNELSRKVLKNWGLNSDLLRAE +AANMQIALGRYDEAI+T
Sbjct: 301 RRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIST 360

Query: 361 LKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 420
           LKDV QHTDKDSE+RGVVF SMGKVLCNQEKFTD KRCLEIA GIFDKRE TSP  AAEA
Sbjct: 361 LKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAEA 420

Query: 421 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           YSEISM YETMN+F+TAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAIP
Sbjct: 421 YSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAIP 480

Query: 481 YLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540
           YLEGAAETL+E FGS HFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540

Query: 541 DSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQG 600
           DSIEACHNLSKAYG MGSYALAIEFQ+QVVDAW+SHGS+AEDELREAR+TL+QLKRKAQG
Sbjct: 541 DSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQG 600

Query: 601 TSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 634
           TSKFE STKALPLPHSSTSSKT QPDLP  QKHQ
Sbjct: 601 TSKFELSTKALPLPHSSTSSKTLQPDLPAHQKHQ 634

BLAST of Chy1G015130 vs. NCBI nr
Match: KAA0059648.1 (nephrocystin-3 [Cucumis melo var. makuwa] >TYK08180.1 nephrocystin-3 [Cucumis melo var. makuwa])

HSP 1 Score: 1131 bits (2925), Expect = 0.0
Identity = 590/634 (93.06%), Postives = 608/634 (95.90%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSYPVSTPNLKPCTKNNGLFFKFTR 60
           MRRNASIS+LPYFNRLNLRSLSSLISPNSISNLP NS PVSTPNLKPCTKNNGLFFKFTR
Sbjct: 1   MRRNASISVLPYFNRLNLRSLSSLISPNSISNLPLNSSPVSTPNLKPCTKNNGLFFKFTR 60

Query: 61  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEKD 120
           FQAN SRNLDTLVEKRPE+SSRQRKLK+KS LEE+FESADTVDEM KAFK MESAFEEKD
Sbjct: 61  FQANSSRNLDTLVEKRPEMSSRQRKLKEKSDLEEAFESADTVDEMVKAFKNMESAFEEKD 120

Query: 121 LGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180
           LGLASLKI LKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK
Sbjct: 121 LGLASLKIGLKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 180

Query: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKC 240
           RFSDSLGYLNRANRILGQLEEKGFSAED+RPVLHAV LELANVKTAMGRREEALSNLRK 
Sbjct: 181 RFSDSLGYLNRANRILGQLEEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRKS 240

Query: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300
           LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD
Sbjct: 241 LEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHD 300

Query: 301 RRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAINT 360
           RRLLGVIYSGLEE+QKALEQNELSRKVLKNWGLNSDLLRAE +AANMQIALGRYDEAI+T
Sbjct: 301 RRLLGVIYSGLEEHQKALEQNELSRKVLKNWGLNSDLLRAEIEAANMQIALGRYDEAIST 360

Query: 361 LKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAEA 420
           LKDV QHTDKDSE+RGVVF SMGKVLCNQEKFTD KRCLEIA GIFDKRE TSP  AAEA
Sbjct: 361 LKDVVQHTDKDSEIRGVVFTSMGKVLCNQEKFTDGKRCLEIAIGIFDKREETSPAAAAEA 420

Query: 421 YSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIP 480
           YSEISM YETMN+F+TAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLL GKVQEAIP
Sbjct: 421 YSEISMLYETMNDFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLIGKVQEAIP 480

Query: 481 YLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540
           YLEGAAETL+E FGS HFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA
Sbjct: 481 YLEGAAETLQESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHA 540

Query: 541 DSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQG 600
           DSIEACHNLSKAYG MGSYALAIEFQ+QVVDAW+SHGS+AEDELREAR+TL+QLKRKAQG
Sbjct: 541 DSIEACHNLSKAYGAMGSYALAIEFQQQVVDAWESHGSTAEDELREARQTLQQLKRKAQG 600

Query: 601 TSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 634
           TSKFE STKALPLPHSSTSSKT QPDLP  QKHQ
Sbjct: 601 TSKFELSTKALPLPHSSTSSKTLQPDLPAHQKHQ 634

BLAST of Chy1G015130 vs. NCBI nr
Match: XP_038899312.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida])

HSP 1 Score: 1083 bits (2802), Expect = 0.0
Identity = 570/635 (89.76%), Postives = 595/635 (93.70%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISN-LPSNSYPVSTPNLKPCTKNNGLFFKFT 60
           MRRNASISLL + NRLNLRSLSS IS NSISN  PSNS PVSTPNLKPCTKN+GLFFKFT
Sbjct: 1   MRRNASISLLSHSNRLNLRSLSSFISSNSISNPSPSNSSPVSTPNLKPCTKNHGLFFKFT 60

Query: 61  RFQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEK 120
           RFQANPSRNLDTLVEKRP+ISSRQRK+K+KS LEE+FESADTVDEM KAFK+MESAFEE+
Sbjct: 61  RFQANPSRNLDTLVEKRPQISSRQRKVKEKSELEEAFESADTVDEMVKAFKDMESAFEER 120

Query: 121 DLGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 180
           DLGLASLKI LKLDQEGEDPE ILLYA+RALKALDKD N NPSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLDQEGEDPENILLYANRALKALDKDVNGNPSLPVAMTLQLMGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRK 240
           KRFSDSLGYLNRANRILGQL EKGFSAED+RPVLHAV LELANVKTAMGRREEALSNLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLVEKGFSAEDVRPVLHAVQLELANVKTAMGRREEALSNLRK 240

Query: 241 CLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 300
           CLEIKEL LEKDSRELG ANRDLAEAYVA+LNFKDALPFCMK LEIHK +LG+NSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGNANRDLAEAYVAVLNFKDALPFCMKALEIHKMQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAIN 360
           DRRLL VIYSGLEE+QKALEQNELS+KVLKNWGLNSDLLRAE DAANMQIALGRY+EAI+
Sbjct: 301 DRRLLAVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYNEAIS 360

Query: 361 TLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAE 420
           TLKDV QHTDKDSE R VVFISMGKVLCNQEKFTD KRCLEIASGIFDKRE TSPVEAAE
Sbjct: 361 TLKDVVQHTDKDSETRAVVFISMGKVLCNQEKFTDPKRCLEIASGIFDKREATSPVEAAE 420

Query: 421 AYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYETMNEF+TAISLLKKSLALLEKLP EQHSEGSVSARLGWLLLLTGKVQEAI
Sbjct: 421 AYSEISMQYETMNEFETAISLLKKSLALLEKLPHEQHSEGSVSARLGWLLLLTGKVQEAI 480

Query: 481 PYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 540
           PYLEGAAETLKE FGS HFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQ 600
           ADSIEAC NLSKAYG MGSY LAIEFQEQVVDAW+SHGS+AEDELREA++ L+QLKRKA 
Sbjct: 541 ADSIEACRNLSKAYGAMGSYPLAIEFQEQVVDAWESHGSTAEDELREAQQMLKQLKRKAH 600

Query: 601 GTSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 634
           GTSK E STKALPLPHSSTSSKT QPDLP  QK Q
Sbjct: 601 GTSKIELSTKALPLPHSSTSSKTLQPDLPARQKRQ 635

BLAST of Chy1G015130 vs. NCBI nr
Match: XP_023549125.1 (protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1040 bits (2689), Expect = 0.0
Identity = 546/635 (85.98%), Postives = 586/635 (92.28%), Query Frame = 0

Query: 1   MRRNASISLLPYFNRLNLRSLSSLISPNSISN-LPSNSYPVSTPNLKPCTKNNGLFFKFT 60
           MRRNASISLL + NR+NLRSLSSLISP  IS+  PS S PVS P+L PCTKN+GLFFKF 
Sbjct: 1   MRRNASISLLFHSNRINLRSLSSLISPKFISDPSPSYSSPVSAPHLNPCTKNHGLFFKFN 60

Query: 61  RFQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEK 120
           RFQA+PSRNL+TLVEKRP+ISSRQRK K+KS LEE+FESA+TVDEM KAFK+MES FEE+
Sbjct: 61  RFQASPSRNLETLVEKRPQISSRQRKFKEKSELEEAFESANTVDEMVKAFKDMESTFEER 120

Query: 121 DLGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSL 180
           DLGLASLKI LKL+QE EDPE+IL+YA RALKALDKDDN  PSLPVAMTLQL+GSVNYSL
Sbjct: 121 DLGLASLKIGLKLEQEDEDPEKILVYATRALKALDKDDNGKPSLPVAMTLQLLGSVNYSL 180

Query: 181 KRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRK 240
           KRFSDSLGYLNRANRILGQLEE GFSAE IRPVLHAV LELANVKTAMGRREEAL+NLRK
Sbjct: 181 KRFSDSLGYLNRANRILGQLEESGFSAEGIRPVLHAVQLELANVKTAMGRREEALANLRK 240

Query: 241 CLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 300
           CLEIKEL LEKDSRELG ANRDLAEAYVAILNFKDALPFCM+ LEIHKK+LG+NSVEVAH
Sbjct: 241 CLEIKELTLEKDSRELGKANRDLAEAYVAILNFKDALPFCMQALEIHKKQLGHNSVEVAH 300

Query: 301 DRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAIN 360
           DRRLLGVIYSGLEE+QKALEQNELS+KVLKNWGLNSDLLRAE DAANMQIALGRYDEAI+
Sbjct: 301 DRRLLGVIYSGLEEHQKALEQNELSQKVLKNWGLNSDLLRAEIDAANMQIALGRYDEAIS 360

Query: 361 TLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAE 420
           TLKDV QHT+KDSE + VVFISMGKVL NQEKF DAKRCLEIA+ IFDK+E  SPV AAE
Sbjct: 361 TLKDVVQHTEKDSETQAVVFISMGKVLYNQEKFADAKRCLEIATRIFDKKEAASPVAAAE 420

Query: 421 AYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 480
           AYSEISMQYE+MNEF+TAISLLK+SLALLEKLPQEQHSEGSVSARLGWLLLLTGKV EAI
Sbjct: 421 AYSEISMQYESMNEFETAISLLKRSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVHEAI 480

Query: 481 PYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 540
           PYLEGAAETLKE FGS HFGVGYIYNNLGAAYLELERPQSAAQMFA+AKDIMDVSLGPHH
Sbjct: 481 PYLEGAAETLKESFGSKHFGVGYIYNNLGAAYLELERPQSAAQMFAMAKDIMDVSLGPHH 540

Query: 541 ADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQ 600
           ADSIEAC NL+KAYG++GSYALAIEFQEQVVDAW+ HGS+AEDELREA+R LEQLKRKA 
Sbjct: 541 ADSIEACQNLAKAYGSLGSYALAIEFQEQVVDAWEGHGSTAEDELREAQRMLEQLKRKAH 600

Query: 601 GTSKFEHSTKALPLPHSSTSSKTPQPDLPTPQKHQ 634
           G SK E S KALPLPHSSTSSKT QPD+P+ QKHQ
Sbjct: 601 GKSKIELSMKALPLPHSSTSSKTLQPDIPSSQKHQ 635

BLAST of Chy1G015130 vs. TAIR 10
Match: AT2G31240.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 746.1 bits (1925), Expect = 2.3e-215
Identity = 395/622 (63.50%), Postives = 491/622 (78.94%), Query Frame = 0

Query: 3   RNASISLLPYFNRLNLRSLSSLISPNSISNLPSNSYPVSTPN--LKPCTKNNGLFFKFTR 62
           ++ SISL+   +   +R +   I  N+ S+  S+S  +S        C + N L  +  +
Sbjct: 2   KSLSISLIRRLHFHKIRPIPLSIHLNAASSSSSSSSLISPAREISTSCRRTNDLIKR--Q 61

Query: 63  FQANPSRNLDTLVEKRPEISSRQRKLKDKSVLEESFESADTVDEMFKAFKEMESAFEEKD 122
           F  NP RNL+T VE+ PEISS     K+K  LEE+FESA+T DEM + FKEME +FE  +
Sbjct: 62  FWVNPYRNLNTHVEEPPEISSSD---KEKIDLEEAFESANTTDEMVRLFKEMELSFEGNE 121

Query: 123 LGLASLKIALKLDQEGEDPEQILLYADRALKALDKDDNRNPSLPVAMTLQLIGSVNYSLK 182
           LGL++LK+ L LD+EGEDPE++L YAD+ALK+ D D N+ P+L VAM  QL+GS NY LK
Sbjct: 122 LGLSALKLGLHLDREGEDPEKVLSYADKALKSFDGDGNK-PNLLVAMASQLMGSANYGLK 181

Query: 183 RFSDSLGYLNRANRILGQLEEKG-FSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRK 242
           RFSDSLGYLNRANRIL +LE  G    ED+RPVLHAV LELANVK AMGRREEA+ NL+K
Sbjct: 182 RFSDSLGYLNRANRILVKLEADGDCVVEDVRPVLHAVQLELANVKNAMGRREEAIENLKK 241

Query: 243 CLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAH 302
            LEIKE+  ++DS+E+G ANR LA+AYVA+LNF +ALP+ +K LEIHKKELGNNS EVA 
Sbjct: 242 SLEIKEMTFDEDSKEMGVANRSLADAYVAVLNFNEALPYALKALEIHKKELGNNSAEVAQ 301

Query: 303 DRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAIN 362
           DRRLLGVIYSGLE++ KALEQN LS++VLKNWG+  +L+RAE DAANM++ALG+Y+EAI+
Sbjct: 302 DRRLLGVIYSGLEQHDKALEQNRLSQRVLKNWGMKLELIRAEIDAANMKVALGKYEEAID 361

Query: 363 TLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKREGTSPVEAAE 422
            LK V Q TDKDSE+R +VFISM K L NQ+KF ++KRCLE A  I +K+E   PVE AE
Sbjct: 362 ILKSVVQQTDKDSEMRAMVFISMSKALVNQQKFAESKRCLEFACEILEKKETALPVEVAE 421

Query: 423 AYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAI 482
           AYSE++MQYE+MNEF+TAISLL+K+L +LEKLPQEQHSEGSVSAR+GWLLL +G+V +A+
Sbjct: 422 AYSEVAMQYESMNEFETAISLLQKTLGILEKLPQEQHSEGSVSARIGWLLLFSGRVSQAV 481

Query: 483 PYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHH 542
           PYLE AAE LKE FG+ HFGVGY+YNNLGAAYLEL RPQSAAQMFAVAKDIMDVSLGP+H
Sbjct: 482 PYLESAAERLKESFGAKHFGVGYVYNNLGAAYLELGRPQSAAQMFAVAKDIMDVSLGPNH 541

Query: 543 ADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQ 602
            DSI+AC NLSKAY  MG+Y+LA+EFQ++V++AWD+HG SA+DE++EA+R LE L+ KA+
Sbjct: 542 VDSIDACQNLSKAYAGMGNYSLAVEFQQRVINAWDNHGDSAKDEMKEAKRLLEDLRLKAR 601

Query: 603 -GTSKFEHSTKALPLPHSSTSS 621
            G S  +   KALPLP  S SS
Sbjct: 602 GGVSTNKLLNKALPLPKPSHSS 617

BLAST of Chy1G015130 vs. TAIR 10
Match: AT3G27960.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 178.3 bits (451), Expect = 1.9e-44
Identity = 137/497 (27.57%), Postives = 236/497 (47.48%), Query Frame = 0

Query: 117 EEKDLGLASLKIALKLDQEGEDPEQILLYADRALKALDK--DDNRNPSLPVAMTLQLIGS 176
           E  +LG+  LK A +L   GE+  + L  A RA+K  +K  +  +   L + M+L ++ +
Sbjct: 149 ESPELGVVLLKQARELVSSGENLNKALDLALRAVKVFEKCGEGEKQLGLNLVMSLHILAA 208

Query: 177 VNYSLKRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEAL 236
           +   L R++D++  L R+  I   + E G   ED      A  ++L ++   MG+ E ++
Sbjct: 209 IYAGLGRYNDAVPVLERSIEI--PMIEDG---EDHALAKFAGCMQLGDMYGLMGQVENSI 268

Query: 237 SNLRKCLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKE--LGN 296
                 LEI+  +L +    +G   R LAEA+V  + F++A   C   L+IHK+      
Sbjct: 269 MLYTAGLEIQRQVLGESDARVGETCRYLAEAHVQAMQFEEASRLCQMALDIHKENGAAAT 328

Query: 297 NSVEVAHDRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALG 356
            S+E A DR+L+G+I     +Y+ ALE   L+   + +     D+   +    +  ++L 
Sbjct: 329 ASIEEAADRKLMGLICDAKGDYEVALEHYVLASMAMSSQNHREDVAAVDCSIGDAYMSLA 388

Query: 357 RYDEAI----NTLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDK 416
           R+DEAI      L    Q   +      +V++ +  +     K  D+K   E A  I+ K
Sbjct: 389 RFDEAIFAYQKALAVFKQGKGETHSSVALVYVRLADLYNKIGKTRDSKSYCENALKIYLK 448

Query: 417 -REGTSPVEAAEAYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGW 476
              GT   E A  + EIS  Y++MNE D A+ LL+++L +    P +Q++   + A++G 
Sbjct: 449 PTPGTPMEEVATGFIEISAIYQSMNELDQALKLLRRALKIYANAPGQQNTIAGIEAQMGV 508

Query: 477 LLLLTGKVQEAIPYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVA 536
           +  + G   E+    + A    +          G   N +G A ++      AA +F  A
Sbjct: 509 VTYMMGNYSESYDIFKSAISKFRNSGEKKTALFGIALNQMGLACVQRYAINEAADLFEEA 568

Query: 537 KDIMDVSLGPHHADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREA 596
           K I++   GP+H D++    NL+  Y  MG    AIE  E VV   +    +A  E+ + 
Sbjct: 569 KTILEKECGPYHPDTLAVYSNLAGTYDAMGRLDDAIEILEYVVGTREEKLGTANPEVEDE 628

Query: 597 RRTLEQLKRKA-QGTSK 604
           ++ L  L ++A +G SK
Sbjct: 629 KQRLAALLKEAGRGRSK 640

BLAST of Chy1G015130 vs. TAIR 10
Match: AT4G10840.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 169.1 bits (427), Expect = 1.2e-41
Identity = 159/619 (25.69%), Postives = 280/619 (45.23%), Query Frame = 0

Query: 29  SISNLPSNSYPVSTPNLKPCTKNNGLFFKFTRFQANPSRNLDTLVEKRPEISSRQRKLKD 88
           ++  L S   P  TP L+    +       T+  +NP R   T ++K P  SS   + K 
Sbjct: 3   AMPGLVSVKTPSDTPPLRVAVPD-------TQPLSNPPR---TPMKKTP--SSTPSRSKP 62

Query: 89  KSVLEESFESADTVDEMFKAFKEMES-AFEEKDLGLASLKIALKLDQEGEDPEQILLYAD 148
                   + + TV     A  +++  + +  DLG   LK+A      GE P + L YA 
Sbjct: 63  SPNRSTGKKDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAI 122

Query: 149 RALKALDK-----------DDNRNPSLPVAMTLQLIGSVNYSLKRFSDSLGYLNRANRIL 208
           RA K+ ++             +  P L +AM+L ++ ++  SL RF +++  L RA ++ 
Sbjct: 123 RATKSFERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVP 182

Query: 209 GQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKCLEIKELMLEKDSRELG 268
                      D      +  ++L +  + +G+ + +++   + L+I+   L      +G
Sbjct: 183 DPTR-----GPDHSLAAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVG 242

Query: 269 TANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHDRRLLGVIYSGLEEYQK 328
              R LAEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +Y+ 
Sbjct: 243 ETCRYLAEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYEN 302

Query: 329 ALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAI----NTLKDVAQHTDKDS 388
           ALE   L+   +   G  S++   +    N+ ++L R+DEA+      L        +  
Sbjct: 303 ALEHLVLASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETH 362

Query: 389 ELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKR-EGTSPVEAAEAYSEISMQYETM 448
                VF+ + ++     K  ++K   E A  I++K   GT+  E A   +EIS  YE++
Sbjct: 363 PTVASVFVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESV 422

Query: 449 NEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKE 508
           +E + A+ LL+KS+ LLE  P +Q +   + AR+G +    G+ ++A    E A   L+ 
Sbjct: 423 DEPEEALKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRA 482

Query: 509 CFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHADSIEACHNLSK 568
             G      G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+ 
Sbjct: 483 A-GEKSAFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAA 542

Query: 569 AYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELREARRTLEQLKRKAQGTSKFEHST--- 624
            Y  MG    AIE  EQV+   +    +A  +  + ++ L +L ++A  +  ++  +   
Sbjct: 543 TYDAMGRIEDAIEILEQVLKLREEKLGTANPDFEDEKKRLAELLKEAGRSRNYKAKSLQN 602

BLAST of Chy1G015130 vs. TAIR 10
Match: AT1G27500.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 166.4 bits (420), Expect = 7.5e-41
Identity = 132/492 (26.83%), Postives = 232/492 (47.15%), Query Frame = 0

Query: 113 ESAFEEKDLGLASLKIALKLDQEGEDPEQILLYADRALKALDKD-DNRNPSLPVAMTLQL 172
           E + E  +L    L  A  L   G+   + L    RA K  +   +N  P L   M L +
Sbjct: 138 EPSSENVELARFLLNQARNLVSSGDSTHKALELTHRAAKLFEASAENGKPCLEWIMCLHV 197

Query: 173 IGSVNYSLKRFSDSLGYLNRANRILGQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRRE 232
             +V+  LK +++++  L R+  I   + E+G   E+      A L++L +    +G+ E
Sbjct: 198 TAAVHCKLKEYNEAIPVLQRSVEI--PVVEEG---EEHALAKFAGLMQLGDTYAMVGQLE 257

Query: 233 EALSNLRKCLEIKELMLEKDSRELGTANRDLAEAYVAILNFKDALPFCMKGLEIHKKELG 292
            ++S   + L I++ +L ++   +G   R LAEA V  L F +A   C   L IH++   
Sbjct: 258 SSISCYTEGLNIQKKVLGENDPRVGETCRYLAEALVQALRFDEAQQVCETALSIHRESGL 317

Query: 293 NNSVEVAHDRRLLGVIYSGLEEYQKALEQNELSRKVLKNWGLNSDLLRAETDAANMQIAL 352
             S+  A DRRL+G+I     +++ ALE   L+   +   G  S++   +T   +  ++L
Sbjct: 318 PGSIAEAADRRLMGLICETKGDHENALEHLVLASMAMAANGQESEVAFVDTSIGDSYLSL 377

Query: 353 GRYDEAI----NTLKDVAQHTDKDSELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFD 412
            R+DEAI     +L  +     ++    G V+I +  +     K  +AK   E A  I++
Sbjct: 378 SRFDEAICAYQKSLTALKTAKGENHPAVGSVYIRLADLYNRTGKVREAKSYCENALRIYE 437

Query: 413 KRE-GTSPVEAAEAYSEISMQYETMNEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLG 472
                 SP E A   ++IS+  E+MNE + AI+LL+K+L +    P ++     + A++G
Sbjct: 438 SHNLEISPEEIASGLTDISVICESMNEVEQAITLLQKALKIYADSPGQKIMIAGIEAQMG 497

Query: 473 WLLLLTGKVQEAIPYLEGAAETLKECFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAV 532
            L  + GK  E+    + A   L+          G   N +G A ++L+  + A ++F  
Sbjct: 498 VLYYMMGKYMESYNTFKSAISKLRATGKKQSTFFGIALNQMGLACIQLDAIEEAVELFEE 557

Query: 533 AKDIMDVSLGPHHADSIEACHNLSKAYGTMGSYALAIEFQEQVVDAWDSHGSSAEDELRE 592
           AK I++   GP+H +++    NL+ AY  +G    AI+    VV   +    +A     +
Sbjct: 558 AKCILEQECGPYHPETLGLYSNLAGAYDAIGRLDDAIKLLGHVVGVREEKLGTANPVTED 617

Query: 593 ARRTLEQLKRKA 599
            +R L QL ++A
Sbjct: 618 EKRRLAQLLKEA 624

BLAST of Chy1G015130 vs. TAIR 10
Match: AT4G10840.2 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 3.1e-39
Identity = 144/546 (26.37%), Postives = 249/546 (45.60%), Query Frame = 0

Query: 29  SISNLPSNSYPVSTPNLKPCTKNNGLFFKFTRFQANPSRNLDTLVEKRPEISSRQRKLKD 88
           ++  L S   P  TP L+    +       T+  +NP R   T ++K P  SS   + K 
Sbjct: 3   AMPGLVSVKTPSDTPPLRVAVPD-------TQPLSNPPR---TPMKKTP--SSTPSRSKP 62

Query: 89  KSVLEESFESADTVDEMFKAFKEMES-AFEEKDLGLASLKIALKLDQEGEDPEQILLYAD 148
                   + + TV     A  +++  + +  DLG   LK+A      GE P + L YA 
Sbjct: 63  SPNRSTGKKDSPTVSSSTAAVIDVDDPSLDNPDLGPFLLKLARDAIASGEGPNKALDYAI 122

Query: 149 RALKALDK-----------DDNRNPSLPVAMTLQLIGSVNYSLKRFSDSLGYLNRANRIL 208
           RA K+ ++             +  P L +AM+L ++ ++  SL RF +++  L RA ++ 
Sbjct: 123 RATKSFERCCAAVAPPIPGGSDGGPVLDLAMSLHVLAAIYCSLGRFDEAVPPLERAIQVP 182

Query: 209 GQLEEKGFSAEDIRPVLHAVLLELANVKTAMGRREEALSNLRKCLEIKELMLEKDSRELG 268
                      D      +  ++L +  + +G+ + +++   + L+I+   L      +G
Sbjct: 183 DPTR-----GPDHSLAAFSGHMQLGDTLSMLGQIDRSIACYEEGLKIQIQTLGDTDPRVG 242

Query: 269 TANRDLAEAYVAILNFKDALPFCMKGLEIHKKELGNNSVEVAHDRRLLGVIYSGLEEYQK 328
              R LAEAYV  + F  A   C K LEIH+      S+E A DRRL+ +I     +Y+ 
Sbjct: 243 ETCRYLAEAYVQAMQFNKAEELCKKTLEIHRAHSEPASLEEAADRRLMAIICEAKGDYEN 302

Query: 329 ALEQNELSRKVLKNWGLNSDLLRAETDAANMQIALGRYDEAI----NTLKDVAQHTDKDS 388
           ALE   L+   +   G  S++   +    N+ ++L R+DEA+      L        +  
Sbjct: 303 ALEHLVLASMAMIASGQESEVASIDVSIGNIYMSLCRFDEAVFSYQKALTVFKASKGETH 362

Query: 389 ELRGVVFISMGKVLCNQEKFTDAKRCLEIASGIFDKR-EGTSPVEAAEAYSEISMQYETM 448
                VF+ + ++     K  ++K   E A  I++K   GT+  E A   +EIS  YE++
Sbjct: 363 PTVASVFVRLAELYHRTGKLRESKSYCENALRIYNKPVPGTTVEEIAGGLTEISAIYESV 422

Query: 449 NEFDTAISLLKKSLALLEKLPQEQHSEGSVSARLGWLLLLTGKVQEAIPYLEGAAETLKE 508
           +E + A+ LL+KS+ LLE  P +Q +   + AR+G +    G+ ++A    E A   L+ 
Sbjct: 423 DEPEEALKLLQKSMKLLEDKPGQQSAIAGLEARMGVMYYTVGRYEDARNAFESAVTKLRA 482

Query: 509 CFGSNHFGVGYIYNNLGAAYLELERPQSAAQMFAVAKDIMDVSLGPHHADSIEACHNLSK 558
             G      G + N +G A ++L +   A ++F  A+ I++   GP   D++    NL+ 
Sbjct: 483 A-GEKSAFFGVVLNQMGLACVQLFKIDEAGELFEEARGILEQERGPCDQDTLGVYSNLAA 530

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LII82.7e-4327.57Protein KINESIN LIGHT CHAIN-RELATED 2 OS=Arabidopsis thaliana OX=3702 GN=KLCR2 P... [more]
O816291.6e-4025.69Protein KINESIN LIGHT CHAIN-RELATED 1 OS=Arabidopsis thaliana OX=3702 GN=KLCR1 P... [more]
F4HSX91.1e-3926.83Protein KINESIN LIGHT CHAIN-RELATED 3 OS=Arabidopsis thaliana OX=3702 GN=KLCR3 P... [more]
A0JM236.7e-1822.63Nephrocystin-3 OS=Xenopus tropicalis OX=8364 GN=nphp3 PE=2 SV=2[more]
Q6AZT73.3e-1722.37Nephrocystin-3 OS=Xenopus laevis OX=8355 GN=nphp3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0K8P30.0e+0097.79TPR_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G375840 ... [more]
A0A1S3BR150.0e+0093.06nephrocystin-3 OS=Cucumis melo OX=3656 GN=LOC103492605 PE=4 SV=1[more]
A0A5A7UWT80.0e+0093.06Nephrocystin-3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold886G00740... [more]
A0A6J1GPX71.0e-30085.98protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita moschata OX=3662 GN=LOC111456... [more]
A0A6J1JWL03.2e-29785.53protein KINESIN LIGHT CHAIN-RELATED 1 OS=Cucurbita maxima OX=3661 GN=LOC11148813... [more]
Match NameE-valueIdentityDescription
XP_004148956.20.097.79protein KINESIN LIGHT CHAIN-RELATED 1 [Cucumis sativus] >KGN44742.1 hypothetical... [more]
XP_008451256.10.093.06PREDICTED: nephrocystin-3 [Cucumis melo][more]
KAA0059648.10.093.06nephrocystin-3 [Cucumis melo var. makuwa] >TYK08180.1 nephrocystin-3 [Cucumis me... [more]
XP_038899312.10.089.76protein KINESIN LIGHT CHAIN-RELATED 1 [Benincasa hispida][more]
XP_023549125.10.085.98protein KINESIN LIGHT CHAIN-RELATED 1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT2G31240.12.3e-21563.50Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT3G27960.11.9e-4427.57Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.11.2e-4125.69Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT1G27500.17.5e-4126.83Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT4G10840.23.1e-3926.37Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 577..601
NoneNo IPR availablePFAMPF13424TPR_12coord: 216..277
e-value: 3.1E-7
score: 30.6
coord: 465..527
e-value: 6.0E-9
score: 36.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 611..634
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 591..634
NoneNo IPR availablePANTHERPTHR47459:SF1KINESIN LIGHT CHAIN-RELATEDcoord: 3..622
NoneNo IPR availablePANTHERPTHR47459KINESIN LIGHT CHAIN-RELATEDcoord: 3..622
IPR019734Tetratricopeptide repeatSMARTSM00028tpr_5coord: 214..247
e-value: 6.0
score: 15.8
coord: 459..492
e-value: 180.0
score: 3.9
coord: 298..331
e-value: 330.0
score: 1.6
coord: 418..451
e-value: 3.5
score: 16.6
coord: 501..534
e-value: 150.0
score: 4.7
coord: 376..409
e-value: 71.0
score: 7.5
coord: 543..576
e-value: 68.0
score: 7.7
coord: 166..199
e-value: 220.0
score: 3.2
coord: 338..371
e-value: 150.0
score: 4.6
coord: 256..289
e-value: 84.0
score: 6.8
IPR019734Tetratricopeptide repeatPFAMPF13181TPR_8coord: 544..571
e-value: 0.0019
score: 18.3
IPR019734Tetratricopeptide repeatPROSITEPS50005TPRcoord: 418..451
score: 8.6144
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 249..413
e-value: 7.7E-14
score: 53.4
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 89..248
e-value: 1.8E-7
score: 33.1
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 415..611
e-value: 3.4E-28
score: 100.5
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 416..564
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 215..406

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G015130.1Chy1G015130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity