Chy1G009970 (gene) Cucumber (hystrix) v1

BLAST of Chy1G009970 vs. ExPASy Swiss-Prot
Match: Q9XID0 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D OS=Arabidopsis thaliana OX=3702 GN=FAB1D PE=3 SV=1)

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 704/1537 (45.80%), Postives = 928/1537 (60.38%), Query Frame = 0

Query: 57   PTLSPMPSLSSCNSDCSTVANLDVR--DGQESATGSQEDIDCRQE----RMSRNSSEVAH 116
            P+ S   S  S + +CS   N   R  + + S+  SQED++  +E    R+ R S  +  
Sbjct: 3    PSNSLSSSERSLSGECSVDGNSCDRGIEDECSSHSSQEDVELTKEVKVDRLERKSKSM-- 62

Query: 117  SNDQLSGRDDGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEG 176
             +D L   D+ S   NS E  Q  S    ++D         D  +W+PPEPE+PED ++G
Sbjct: 63   PSDILDILDEKS-KENSVENVQFLSDREDDSD---------DVPVWEPPEPENPEDEVDG 122

Query: 177  GMGYNDDDDDEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIRDL 236
                  DDDD+  D S+W+ AS      DE+S   +  EE +R + +  + K K  +  L
Sbjct: 123  VFA---DDDDDCCDGSKWNKASLLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQL 182

Query: 237  LKLG----EDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFV 296
            +K      E+S  W +IV+ L WEAA+ LKP ++GK++DP   +KVKCIATG+   S+  
Sbjct: 183  IKSAGFSIEESGYWFEIVARLCWEAASMLKPAIDGKSVDPTEYIKVKCIATGSCVDSEVF 242

Query: 297  KGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQEND----FTNHVIE 356
            KG+VFKKHAA KHM T  ++P+++L++G+LG  PIS  SS  S++Q+N+    +   V++
Sbjct: 243  KGLVFKKHAALKHMATKYEHPRIMLVEGVLGH-PISGFSSLQSVNQDNEYLLKYVKPVVD 302

Query: 357  MIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQ 416
            +IE    +V+LVEK+ +R IQ+ IL KG+TLV DMKLHRLQRI+ C GSPIL+ ++L SQ
Sbjct: 303  IIEASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPILSVDSLSSQ 362

Query: 417  KTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKI 476
            K + CD+   +KIVEEH    E  K PTKTLMF+EGC TRLGCTILLKG HS+ LK+VK 
Sbjct: 363  KLKHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKE 422

Query: 477  VVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESAS 536
            VVQ + ++A+HL+LE SF+ D+  MF+TI      A  ++     +E  SP+    ES S
Sbjct: 423  VVQYSFILAYHLMLEASFLADRHTMFSTI-----FAKEATSCVVEIENFSPSPSPRESPS 482

Query: 537  DKSEQPTSIYISRGSHEEASTGSNMELAEKLIMSSEP--------EPYNPAIFSGFSSIS 596
            +  + P S        +E +   N E   + + + E         EPYNP IF+GFSS+S
Sbjct: 483  EAVDIPVS-----NGFDEQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLS 542

Query: 597  DSLKRVMGESFLSSSPYQSLSSYFGHGSDLSGLIAKSSSIPSTPQ----AVYQFDVEVRG 656
              L + +G      S   S+ +     S+L  +           +     +   ++ V  
Sbjct: 543  ARLSKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEEDTAEKNEDKQPLLLDPELPVNS 602

Query: 657  SSDEENSVHEQSVSHQSTLEGLGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGA 716
            SSD+ ++  +     +STLE                       SQSILVL+S RNAL+G 
Sbjct: 603  SSDDGDNKSQTENDIESTLE-----------------------SQSILVLVSKRNALRGI 662

Query: 717  MCEQSHFSHIVFYKHFDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQV 776
            MC+Q HFSHI FYKHFDVPL KFL+D + NQ NLC  C   PEAH Y+YAH  KQL+IQ+
Sbjct: 663  MCDQRHFSHIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQI 722

Query: 777  KQLPGDKVLPGETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSD 836
            K++P  K L GE +GK+WMWSRC KCK+     KSTKRVLISTAAR LSFGKFLEL FS 
Sbjct: 723  KRIPVAKGLAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQ 782

Query: 837  DTLLGKSSVCGHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLI 896
             T L +SS CGHS   DFL+FFGLG+MVAM  YS+VA YTVS+PP KLE +  ++ G L 
Sbjct: 783  QTFLNRSSSCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLE 842

Query: 897  RETENVYTKGVLLFTEIAHSLKKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELN 956
            +E + V+TKG+ LF + A  LK++ S+  +S++ + Q      S +EE+L  ER  FE N
Sbjct: 843  KEFQTVFTKGISLFEDAAGFLKRLRSQFTNSDLRY-QRARKLLSNIEELLKHERCIFEEN 902

Query: 957  IQNSLTKKGNPD-----FLNLNRLLWDLLLESCIWDRRLQSL-RSLGVTTNSGSSEIVEP 1016
            I+NS  K    D      L LNR+ W+LLL++ IW+ RLQSL  S  +  +S  ++I E 
Sbjct: 903  IKNSFDKAKTIDDVSHRLLRLNRMRWELLLQALIWNYRLQSLVLSDRLLPSSDETKIYEQ 962

Query: 1017 EPLLLKMNSNIDVGPEELESTAENDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELN 1076
                LK  S       E   T   +D KV    S      PL E     + G S GD   
Sbjct: 963  G---LKTVS-------EAGMTRYENDNKVSDSGSNGGIDTPLVEHKDIPIAGASVGDNDQ 1022

Query: 1077 LPSAI--EVTEIPIIDDLSPKKLSRQGTLSNGFDYH-----HSDYEDSQVGKVLSSGDLH 1136
            +  +   E  E   +   SP   S    ++N FD H     HS         +  +G+  
Sbjct: 1023 MAESYVPEDNESQTLCSSSPDTTS---PINNHFDTHLAVNVHSTNGQEADKSIPVTGE-S 1082

Query: 1137 IDRTIPISIGDSTL--SKLFWTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLPAASDF 1196
            +D  +  S G   L   + FW PF E+R   + DI++ Y  + + ++++T + L   +  
Sbjct: 1083 LDDEVSTSNGPHILGWDEWFWLPFEELRSKRIVDIEKEYLLKFEYVNNFTQENLQTVNQI 1142

Query: 1197 IHEEGQKLHIHLDNDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQ 1256
            I EE  +L I L +D+F+VSDYE ELSS++AC LA L         +NE+SK      + 
Sbjct: 1143 ITEESSRLRISLRDDDFIVSDYEDELSSLIACALAHL---------NNEESKKPLSRCIH 1202

Query: 1257 PALSSRSLNKVPSNGSSDSDSSISSEEYRFSSFDRLNLLDSL----VSETFKRSDHEGVI 1316
             +L     N   +  S  +D  +S    RFSS +  N L++L    V  TF      G +
Sbjct: 1203 GSLQGFLDN---NQDSKQTDRDVS----RFSS-ESTNRLETLPPPEVLVTF------GSV 1262

Query: 1317 KSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDR 1376
            KS+ K KY +   Y + FRDLR RCC SEL +IASLSRC  W+AKGGKSKS FAKTLDDR
Sbjct: 1263 KSVGKPKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDR 1322

Query: 1377 FIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREPK-SGKEMRH 1436
            FI+KEIK+TEY+SF+ FA EYFKY+ +S+D+GNQTCLAKVLGI+QVTVR+PK  GKE+RH
Sbjct: 1323 FIVKEIKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRH 1382

Query: 1437 DLMVMENLSFGRNIIRQYDLKGALHARFN-PATNNFGEVLLDQNFVNDMNSSPLYVSNRA 1496
            DLMVMENLSF R + RQYDLKGALHARF   + N   +VLLDQNFVNDMN SPLYVS  +
Sbjct: 1383 DLMVMENLSFSRKVTRQYDLKGALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTS 1442

Query: 1497 KRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSS 1546
            K+ LQRA++NDT+FL SINVMDYSLLVGVD E  ELVCGIIDYLRQYTWDKQLETWVKSS
Sbjct: 1443 KQNLQRAVYNDTSFLTSINVMDYSLLVGVDDENHELVCGIIDYLRQYTWDKQLETWVKSS 1451

BLAST of Chy1G009970 vs. ExPASy Swiss-Prot
Match: Q9SSJ8 (Putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1C OS=Arabidopsis thaliana OX=3702 GN=FAB1C PE=2 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 2.9e-216
Identity = 525/1441 (36.43%), Postives = 783/1441 (54.34%), Query Frame = 0

Query: 155  LWDPPEPEDPEDHMEGGMGYNDDDDDEFGDN-SEWSTASSFSRSVDEASVSYRFKEEKQR 214
            +W PP PED  D  E    + DD+DD+ GD+ +E+S +SSFS  +       +  E    
Sbjct: 263  IWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHI---PTKEKLGENSNE 322

Query: 215  ALQQVMNGKHKAFIRDLLKLGED--------SENWVDIVSSLSWEAATFLKPVVN-GKAM 274
             L+ V++   +A + +LL+ GE+        +  W+DIV++L+W+AA F+KP    G +M
Sbjct: 323  PLRTVVHDHFRALVAELLR-GEELSPSDDGSAGEWLDIVTALAWQAANFVKPDTRAGGSM 382

Query: 275  DPDANVKVKCIATGTRNQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPIS-R 334
            DP   VK+KC+A+G +N+S  ++G+V  K+  HK M +  KNP+++L+ G L    ++ +
Sbjct: 383  DPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLEYQRVAGQ 442

Query: 335  LSSFNS-MDQENDFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRL 394
            L+SFN+ + QEN+    +I  IE    NV+LVEK+A+   Q+ +L+K ++LVL++K   L
Sbjct: 443  LASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVLNVKRSLL 502

Query: 395  QRIALCTGSPILTS-ETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCST 454
             RIA CTG+ +  S +++ + +   C+    ++++E+H    +  + P++TLM+ EGC  
Sbjct: 503  DRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGNQSNRKPSRTLMYFEGCPR 562

Query: 455  RLGCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETSFIVDQRAMFATIPF--GGVPAT 514
            RLGCT++L+G+  +ELK+VK V+Q AV  A+HL LETSF+ D+ A    I     G+  T
Sbjct: 563  RLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPKIRLKQPGMVRT 622

Query: 515  ASSDPQSPLEPRSPNVGKAESASDKSEQPTSIYISRGSHEEASTGSNMELAEKLIMSSEP 574
            AS       + R  + G +      +E  +   +   +HE+  T     + E  +  S  
Sbjct: 623  AS-------QRRIIDEGISLITQSPTETDSQALLETAAHEDEHTA---PMPEHEVCESLC 682

Query: 575  EPYNPA-IFSGFSSISDSLKRVMGESFLSSSPYQSLSSYFGHGSDL-SGLIAKSSSIPST 634
            E ++P  IF   S +       +   F ++   +S SS     +DL    +  SS IP T
Sbjct: 683  EDFDPTQIFPPSSEVETEQSDTLNGDFANNLVTRSYSS--NQLNDLHEPTLCLSSEIPET 742

Query: 635  PQAVYQFDVEVRGSSDEENSVHEQSVSHQSTLEGLGFHETAPNYSVDTMQKKMSLDSQSI 694
            P      + E  G  +EEN    Q V+ Q     L  HE+     V +     +   QSI
Sbjct: 743  PTQQPSGE-EDNGRGEEEN----QLVNPQD----LPQHESFYEDDVSSEYFSAADSHQSI 802

Query: 695  LVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFY 754
            LV  SSR  LK ++CE+S    I FY  FD PLG++L+D+L ++ + C  C  L +AH  
Sbjct: 803  LVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELVDAHVL 862

Query: 755  FYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARG 814
             Y+H    L+I V++LP  K LPGE +GK+WMW RC +C    G   +T+RV++S AA G
Sbjct: 863  CYSHQNGNLTINVRRLPSMK-LPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSDAAWG 922

Query: 815  LSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQK 874
            LSFGKFLEL FS+     + + CGHSL  D L F+G GNMVA  RYS + I TV +PP  
Sbjct: 923  LSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLLPPSM 982

Query: 875  LEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSLKKIISERKSSNVNHPQCFPNDFSF-- 934
            LEFNS  +Q  +  E   +  K   ++TEI+  L ++  E KSS +   Q    D     
Sbjct: 983  LEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRM--EEKSSLLEPEQSEACDLHSRI 1042

Query: 935  --VEEMLNEERSEFELNIQ----NSLTKKGNPDFLNLNRLLWDLLLESCIWDRRLQSLRS 994
              + + L +E+ E++  +Q     +L  +G+ D L LNRL   L++ +  WD +L  L S
Sbjct: 1043 IGLIDQLVKEKDEYDDALQPIFEENLQIQGSLDILELNRLRRALMIGAHAWDHQLYLLNS 1102

Query: 995  ---------LGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELESTAE-NDDTKVQQDISL 1054
                      G      + E+ +P  +  +M    D   E+  + +E N D K  ++I  
Sbjct: 1103 QLKKASVFKTGDDNAPRNPEMHDPPKIDRRMQEGSDERDEQSHTDSEANGDNKDPENIPS 1162

Query: 1055 EENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIID--DLSPKKLSRQGTLSNGFDY 1114
                L       E +D    G   NL  A  + E       + S ++L+R   + + FD 
Sbjct: 1163 PGTSLS------ERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQS-FDS 1222

Query: 1115 HHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMHLRDIQRSYFPE 1174
                 E  Q G   SS  L   R+   S        +   P S + + + + +      +
Sbjct: 1223 AIRFQERIQKGLPPSSLYLSTLRSFHAS---GEYRNMVRDPVSNVMRTYSQMLPLE-VQK 1282

Query: 1175 LKSISSYTPKLLPAASDFIHEEGQKLHI-HLDNDNFVVSDYEGELSSIVACVLALLKDQP 1234
            L  I    P  + +AS     +G ++ I     ++ VV  Y+ + +S+V+  +       
Sbjct: 1283 LDLIVGSAPTYISSASQM--ADGARMLIPQRGLNDIVVPVYDDDPASVVSYAI------- 1342

Query: 1235 FQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSD--------SSISSEEYRFSSF 1294
              +  + E    +G   L  + SS +LN   S  S+ S           I    Y  S  
Sbjct: 1343 -NSKEYKEWIVNKG---LASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQD 1402

Query: 1295 DRLNLLDSLVSETFKRSDHEGVIKSLAKG--KYLVNCPYINQFRDLRSRCCPSELHFIAS 1354
            DR +        T   SD      +  +G  K+ V C +  QF  LR  CCPSE+ F+ S
Sbjct: 1403 DRKS-----PHLTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRS 1462

Query: 1355 LSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQT 1414
            LSRC  W+A+GGKS  +FAK+LD+RFIIK++ +TE DSF  FAPEYFKY+ ES   G+ T
Sbjct: 1463 LSRCQRWSAQGGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPT 1522

Query: 1415 CLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFG 1474
            CLAK+LGIYQV+++ PK GKE + DLMVMENL + R I R YDLKG+  +R+NP T+   
Sbjct: 1523 CLAKILGIYQVSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGAD 1582

Query: 1475 EVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELV 1534
            +VLLD N +  + + P+++ ++AKR L+RAIWNDT FL S++VMDYSLLVG D E+KELV
Sbjct: 1583 KVLLDMNLLETLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELV 1642

Query: 1535 CGIIDYLRQYTWDKQLETWVKSSLI---PKNVLPTVISPKEYKRRFRKFMSAHFLSVPDH 1545
             GIID++RQYTWDK LETWVK+S I   PKN  PT++SPK+YKRRFRK M+ +FL+VP+ 
Sbjct: 1643 LGIIDFMRQYTWDKHLETWVKASGILGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEP 1646

BLAST of Chy1G009970 vs. ExPASy Swiss-Prot
Match: Q9LUM0 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1B OS=Arabidopsis thaliana OX=3702 GN=FAB1B PE=2 SV=1)

HSP 1 Score: 747.3 bits (1928), Expect = 3.6e-214
Identity = 556/1566 (35.50%), Postives = 805/1566 (51.40%), Query Frame = 0

Query: 84   QESATGSQEDIDCRQERMSRNSSEVAHSNDQLSGRDDGSVMSNSQETTQNASGVPVNADL 143
            Q+S +GS     C  E + R  SE      +  GRD+    S+  + + +    PV  D 
Sbjct: 257  QKSLSGSPLIHQC-LESLIREGSEQFQKKSEHDGRDECEA-SSPADISDDQVVEPV--DF 316

Query: 144  EQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDDDEFGDNSEWS-TASSFSRSVDEAS 203
            E +        LW PPEPE+ ED  E  +   D++D+E   + EW     S S    E  
Sbjct: 317  ENNGL------LWVPPEPENEEDERESAL--FDEEDNEGDASGEWGYLRPSTSFGSGEYR 376

Query: 204  VSYRFKEEKQRALQQVMNGKHKAFIRDLLKL-------GEDSENWVDIVSSLSWEAATFL 263
               R  EE ++A++ V++G  +A +  LL++        E  E+W++I++SLSWEAA  L
Sbjct: 377  GEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANLL 436

Query: 264  KP-VVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQG 323
            KP +     MDP   VKVKC+A+G R+ S  VKG+V KK+  ++ M T  +  +L+++ G
Sbjct: 437  KPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILGG 496

Query: 324  MLGEAPIS-RLSSFNS-MDQENDFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMT 383
             L    +S +LSSF++ + QE D     +  I     N++LVEK+ +R  QE +L K ++
Sbjct: 497  GLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDIS 556

Query: 384  LVLDMKLHRLQRIALCTGSPILTS-ETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMPTK 443
            LVL++K   L RIA CTG+ I+ S + L SQK   C+     +  EEH    + GK   K
Sbjct: 557  LVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGKKVVK 616

Query: 444  TLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETSFIVDQRAMFATI 503
            TLM+ E C   LG TILL+GA+ DELK+VK VVQ  V  A+HL LETSF+ D+ A    +
Sbjct: 617  TLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS-PEL 676

Query: 504  PFGGVPATASSDPQSPLEPRSPNVGKAESASDKSEQPTSIYISRGSH-------EEASTG 563
            P       A  D  + +E RS +     + S   + PT +  +           E  ST 
Sbjct: 677  PLNSPITVALPDKSTSIE-RSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELLSTT 736

Query: 564  SNMELAEKLIMSSEPEPY---------NPAIFSGFSSISDSLKRVMGESFLS-------- 623
            +N+ + + +     P PY         NP+      +IS +L   + ES  S        
Sbjct: 737  TNLSIQKDI----PPIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSGRSVP 796

Query: 624  -------SSPYQSLSSYFGHGSDLS--GLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSV 683
                   S+P         +   LS  G + KSS I ++     Q +      + ++N+ 
Sbjct: 797  VDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNE 856

Query: 684  HEQSVSHQSTLEGLGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFS 743
              +    Q         E  P+ S          D QSILV +SSR+  KG +CE+SH  
Sbjct: 857  KPKETQSQK-------EEFPPSPS----------DHQSILVSLSSRSVWKGTVCERSHLF 916

Query: 744  HIVFYKHFDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKV 803
             I +Y  FD PLG+FL+D+L +Q+  C  C    EAH + Y H +  L+I VK+L  D +
Sbjct: 917  RIKYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQ-DYL 976

Query: 804  LPGETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSS 863
            LPGE EGK+WMW RC +C    G   +T RV++S AA GLSFGKFLEL FS+     + +
Sbjct: 977  LPGEKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVA 1036

Query: 864  VCGHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYT 923
             CGHSL  D L F+G GNMVA  RY+ + +++V +PP  L FN    Q  + RET+ V  
Sbjct: 1037 CCGHSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFNYE-NQDWIQRETDEVIE 1096

Query: 924  KGVLLFTEIAHSLKKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSL--- 983
            +  LLF+E+ +++ +I  +           F      +EE+L +E++EFE N+Q  L   
Sbjct: 1097 RAELLFSEVLNAISQIAEKG----------FRRRIGELEEVLQKEKAEFEENMQKILHRE 1156

Query: 984  TKKGNP--DFLNLNRLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPE-PLLLKMN 1043
              +G P  D L L R+   LL +S +WD RL +  +L    NS  ++  E E P L K  
Sbjct: 1157 VNEGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDTKREENEKPPLAKSQ 1216

Query: 1044 S--NIDVGPEELESTAE---NDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPS 1103
            +   ++ G   L + +E   N D     D     NV    +   +    +  G E++   
Sbjct: 1217 TLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSDLYQEKDDGGEVSPSK 1276

Query: 1104 AIEVTEIPIIDDLSPKKLSRQGTL-------------------------SNGFDYHHSDY 1163
             +  T  P+ + +  ++    G +                         +N      S  
Sbjct: 1277 TLPDTSYPLENKVDVRRTQSDGQIVMKNLSATLDAAWIGERQTSVEIPTNNKVSLPPSTM 1336

Query: 1164 EDSQVGKVLSSGDLHID---------------RTIPISIGDSTLSKLFW--TPFSEIRQM 1223
             +S     +S G + ID                 +P    +++   + W   PF      
Sbjct: 1337 SNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSEDSVSWLSVPFLNF--- 1396

Query: 1224 HLRDIQRSYF---PELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGELS 1283
              R I +++     +L +   ++P  + +  +   + G +L + +  ++ VV  Y+ E +
Sbjct: 1397 -YRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDIVVPVYDDEPT 1456

Query: 1284 SIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEE 1343
            S++A  L +  +   QT +  E           P     ++   PS  +   D SI S  
Sbjct: 1457 SMIAYAL-MSPEYQRQTSAEGESLVSYPSELNIPRPVDDTIFD-PSRSNGSVDESILS-- 1516

Query: 1344 YRFSSFDRLNLLDSL-VSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSEL 1403
               SS    +LLD L  ++        G   +L K KY V C Y  +F  LR  C PSEL
Sbjct: 1517 --ISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICLPSEL 1576

Query: 1404 HFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFD 1463
             +I SLSRC  W A+GGKS  FFAKTLDDRFIIK++ +TE +SF+KFAP YFKY++ES  
Sbjct: 1577 EYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLSESIS 1636

Query: 1464 MGNQTCLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPA 1523
              + TCLAK+LGIYQV  ++ KSGKE + D+++MENL FGR + R YDLKG+  AR+NP 
Sbjct: 1637 TKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRARYNPD 1696

Query: 1524 TNNFGEVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAE 1545
            ++   +VLLDQN +  M +SP++V N+AKR L+RA+WNDTAFL   +VMDYSLLVGVD E
Sbjct: 1697 SSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVGVDEE 1756

BLAST of Chy1G009970 vs. ExPASy Swiss-Prot
Match: Q0WUR5 (1-phosphatidylinositol-3-phosphate 5-kinase FAB1A OS=Arabidopsis thaliana OX=3702 GN=FAB1A PE=2 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 5.7e-212
Identity = 531/1481 (35.85%), Postives = 768/1481 (51.86%), Query Frame = 0

Query: 155  LWDPPEPEDPEDHMEGGMGYNDDDDDEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRA 214
            LW PPEPE+ ED  E  +  +DDD DE GD  +W      S S +E    +  K++   A
Sbjct: 311  LWLPPEPENEEDEREAVL--SDDDGDE-GDRGDWGYLRP-SNSFNEK--DFHSKDKSSGA 370

Query: 215  LQQVMNGKHKAFIRDLLKLGE-------DSENWVDIVSSLSWEAATFLKP-VVNGKAMDP 274
            ++ V+ G  +A +  LL++         D E W+DI++SLSWEAAT LKP       MDP
Sbjct: 371  MKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMDP 430

Query: 275  DANVKVKCIATGTRNQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPIS-RLS 334
               VKVKCI  G R++S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +LS
Sbjct: 431  GGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLS 490

Query: 335  SFNS-MDQENDFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQR 394
            SF++ + QE D     +  I+    +++LVEK+ +R  QE +L K ++LVL++K   L+R
Sbjct: 491  SFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLER 550

Query: 395  IALCTGSPILTS-ETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCSTRL 454
            I+ CTG+ I+ S + L S K   CD  + +K VE H    +  K   KTLMF +GC   L
Sbjct: 551  ISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPL 610

Query: 455  GCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSD 514
            GCTILLKGAH DELK+VK V+Q  V  A+HL LETSF+ D+ A    +P    P T +  
Sbjct: 611  GCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVALP 670

Query: 515  PQSPLEPRSPNVGKAESASDKSEQPTS---------------IYISRGSHEEASTGSN-- 574
             +  +  RS +     + S   + PT+                + S  +H +     N  
Sbjct: 671  DKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGNDR 730

Query: 575  MELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQSLSSYFGHGSDLSG 634
            ++ +E+L+ + +     P      +S  D L   + ES   S   +  S      S LSG
Sbjct: 731  IDPSERLLHNLDTVYCKPP--ETITSKDDGLVPTL-ESRQLSFHVEEPSVQKDQWSVLSG 790

Query: 635  LIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSHQSTLEGL--GFHETAPNYSVD 694
                     +T Q      V   G +++   +  Q+ + Q  +E     FH +A      
Sbjct: 791  ---------ATEQ------VTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHPSAS----- 850

Query: 695  TMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLGKFLQDNLLNQNNL 754
                    D QSILV +S+R   KG++CE++H   I +Y  FD PLG+FL+DNL +Q+  
Sbjct: 851  --------DHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQC 910

Query: 755  CTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSTGGPSK 814
            C  C    EAH + Y H +  L+I VK+LP  ++LPG+ EGK+WMW RC KC    G   
Sbjct: 911  CPSCTMPAEAHIHCYTHRQGSLTISVKKLP--ELLPGQREGKIWMWHRCLKCPRINGFPP 970

Query: 815  STKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYFFGLGNMVAMSRYS 874
            +T+R+++S AA GLSFGKFLEL FS+     + + CGHSL  D L F+G G MVA  RY+
Sbjct: 971  ATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYA 1030

Query: 875  EVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSLKKIISERKSSNVN 934
             + IY V++PP KL FN    Q  L +E++ V  K  +LF E+  +L +I    K+    
Sbjct: 1031 SINIYAVTLPPAKLYFNYE-NQEWLQKESKEVIKKAEVLFNEVQEALSQI--SAKTMGAG 1090

Query: 935  HPQCFPNDFSF-VEE---MLNEERSEFELNIQNSL--TKKGNP--DFLNLNRLLWDLLLE 994
                 PN     +EE   +L + + E++ ++Q  L   K G P  D L +N+L   ++ +
Sbjct: 1091 SKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQMLNVVKDGQPTIDILLINKLRRLIIFD 1150

Query: 995  SCIWDRRLQSLRSL-------------------GVTTNSGSSEIVEPEPLLLKMNSNIDV 1054
            S  WD  L    ++                    V+    S E V+  P  + + ++  +
Sbjct: 1151 SYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAICNDSLL 1210

Query: 1055 GPEELESTAEND----DTKVQQDISLEENVLPLKEITVEGLDGESGGDEL---------- 1114
               + E+         DT  +  I  +       +  +E    E G D            
Sbjct: 1211 QDADYETCLNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDPSEGGKDNFVESSQVVKPA 1270

Query: 1115 NLPSAIEVTEIPIIDDLSPKKLSRQGTLSNGFDYHHSDYEDSQVGKV------------- 1174
            +  S  + T++   D L    +  Q T  NG     S    +   ++             
Sbjct: 1271 HTESQFQATDLS--DTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIPDLRLLGSESELN 1330

Query: 1175 LSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLP 1234
               G  + + T  + +   +        +S   + H+    R  +     +SSY      
Sbjct: 1331 FKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKHIMAEDRPVY-----VSSY------ 1390

Query: 1235 AASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEG 1294
               +     G +L + L  ++ V+  Y+ E +SI+A  L   + +   + S     + + 
Sbjct: 1391 --RELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSGSDKSRDRLDS 1450

Query: 1295 GG--ALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFSSF--DRLNLLDSLVSETFKRSD 1354
            GG  +L  +++  SLN + S+ S D   S+SS + + S      L L D     +F    
Sbjct: 1451 GGSFSLFDSVNLLSLNSL-SDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEG 1510

Query: 1355 HEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAK 1414
              G      K KY V C Y  +F  LR  CCPSE  FI SL RC  W A+GGKS  FFAK
Sbjct: 1511 PPG------KVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAK 1570

Query: 1415 TLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREPKSGK 1474
            +LDDRFIIK++ +TE +SF+KF P YFKY+ ES    + T LAK+LGIYQV+ +  K GK
Sbjct: 1571 SLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGK 1630

Query: 1475 EMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVS 1534
            E + D++VMENL F RN  R YDLKG+  AR+NP T+    VLLDQN V  M +SP++V 
Sbjct: 1631 EFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSPIFVG 1690

Query: 1535 NRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWV 1545
            ++AKR L+RA+WNDT+FL SI+VMDYSLLVGVD E+ ELV GIID++RQYTWDK LETWV
Sbjct: 1691 SKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHLETWV 1726

BLAST of Chy1G009970 vs. ExPASy Swiss-Prot
Match: O59722 (1-phosphatidylinositol 3-phosphate 5-kinase fab1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=fab1 PE=1 SV=2)

HSP 1 Score: 344.0 bits (881), Expect = 9.2e-93
Identity = 378/1487 (25.42%), Postives = 637/1487 (42.84%), Query Frame = 0

Query: 234  GEDSE-NWVDIVSSLSWEAATFLKP-VVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVF 293
            G DS   WV  +  +  + A  L P + +G  +D  + VK+K I  G+      V G++F
Sbjct: 487  GVDSPMQWVLCLPKILLKMALDLGPDIRSGDDIDVRSYVKIKKIPGGSIQDCFLVNGVLF 546

Query: 294  KKHAAHKHMPTHCKNPKLILIQGMLGEAPISR--LSSFNSMDQENDFTNHVIEMIEGCTA 353
             K A+ K M    + P++ L+   L  A   +  LS    + Q+ ++  +++  I     
Sbjct: 547  SKKASSKSMDRSLRRPRIALLTFSLDYACDEQRILSLDLIISQQEEYIINLVNRICMLKP 606

Query: 354  NVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDA 413
            N++  +     +  +   + G+     +K   L  IA C  + I++S   +S   R    
Sbjct: 607  NLVFAQGQIPSIALKYFEEHGVIAFHGLKESVLYDIARCCRADIISSIDKLSLCPRLGTC 666

Query: 414  VYFQ---KIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQC 473
              FQ    +V+E+ G+        KT   ++ CS RLGCTI+L+GA  ++L +VK +V+ 
Sbjct: 667  GRFQLRTYVVDENKGL-------RKTFAILDRCSERLGCTIVLRGADYNQLSKVKKIVEL 726

Query: 474  AVVMAFHLILETSFIVDQ--------RAMFATIPFGGVPATASSDPQSPLEPRSPNVGKA 533
             V++A+H+ LE + + D+           + ++    +P+ AS+   +  E    +  K+
Sbjct: 727  VVLIAYHIKLECALLRDKFVNMPELFETTYQSLSRKSLPSFAST--AADKEKSQNHEKKS 786

Query: 534  ESASDKSEQPTSIYISRGSHEEASTGSN--MELAEKLIMSSEPEPYNPAIFSGFSSISDS 593
             ++ ++S +P     +      ++ GSN  +EL   L  S +           +SSI+ +
Sbjct: 787  LNSDNQSLRPLE---NENQSVSSTQGSNSPLELINNLPASDD-----------YSSITKA 846

Query: 594  LK-RVMGESFLSSSPYQSLSSYFGHGSDLSGLIAKSSSI-PSTPQAVYQFDVEVRGSSDE 653
            LK R +  S   S P   L +   +   +   + K   + P +P   +     V   S+ 
Sbjct: 847  LKTRFLTFSPFLSKPLPRLLNQVNYYQFIRNKLLKDVKLHPYSPTGSF-----VMKQSEN 906

Query: 654  ENSVHEQSVSHQSTLEGLGFHETAPNYSVDTMQKKM---SLDSQSILVLMSSRNALKGAM 713
            +N       S++       +H     +++     K+      SQ I++L S  N      
Sbjct: 907  DNVEESYEESYKFFCIDERYHFLEKQWTLYYSHSKLMFSPFSSQRIILLYSIINKETSVP 966

Query: 714  CEQSHFSHIVFYKHFDVPLGKFLQDNLLNQNNLCTVCGGL------PEAHFYFYAHYRKQ 773
            C       + FY+  D  LG++++D+ LN N     CGG          H+  Y H   +
Sbjct: 967  CIGPERCLLEFYRETDCTLGQYIEDSCLNTN---VSCGGEYCKTNDMLWHYRSYVHGNSR 1026

Query: 774  LSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLE 833
            +S+ ++       +PG  E K+ MWS C  CK      K+T   ++S      SFGK+LE
Sbjct: 1027 ISVFLESF--SCPVPG-LEEKIIMWSYCKFCK------KNTHITVMSEETWKYSFGKYLE 1086

Query: 834  LCFSDDTLLGKSSVCGHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMR 893
              F +  +  +   C HS+    +++FG  NM    +   + I+ + +P   L  N S  
Sbjct: 1087 FMFYNSQIRDRFEFCDHSVMAQHVHYFGYCNMALRFQRDLIEIFELFVPSVTLRNNPSYI 1146

Query: 894  QGHLIRETENVYTKGVLLFTEIAHSLKKIISERKSSNVNHPQCF---PNDFSFVEEMLNE 953
            +   ++E E    KGV+   +   S+   I++ K   V  P+ F    ++ S    +L+ 
Sbjct: 1147 KE--LKEKEYKRLKGVI--EKCLSSVASRINQIKCDWVTDPEKFESCTSEISKFRTLLSS 1206

Query: 954  ERSEFELNIQNSLTKKGNPDFLNLNRLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIV 1013
            + +E      +        D+L+LN +L  L  +   W++R    + L + +    S+I 
Sbjct: 1207 DYTELYSEFDSIYLNSSTSDYLSLNSILRVLQGKMVKWEQRFLDYQRLYLPSYKELSKIA 1266

Query: 1014 EPE-------------PLLLK---MNSNIDVGPE-ELESTAENDDTKVQQDISLEENVLP 1073
              +             PL L     N+ ID+ P  + EST +  +   Q +++  + V P
Sbjct: 1267 AAQIKKVFLERPLSQTPLDLPETLENTQIDIYPSFKTESTDDQLEKVTQTNVASNKRVAP 1326

Query: 1074 LKEITVEGLDGESGGDELNLPSAIEVTEIPIIDDLS--------------------PKKL 1133
              +        ES    +   S I    I   +D+S                    P   
Sbjct: 1327 YADSMANVGSPESDCFSVATSSDIPKANIDFTNDISTQNTFPASPVSNSGFSRQTYPNIS 1386

Query: 1134 SRQGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQM 1193
             RQG   N   +       S    V +S    ++  I  SI     S    T +S  + +
Sbjct: 1387 QRQGV--NMLSHKRKSASTSDRRFVNASSTSGMNMPISSSISAKISSIQNSTKYSPRKPI 1446

Query: 1194 HLRDIQRS----YFPEL------------KSISSYTPKLLPAA-SDFIHEEGQKLHIHLD 1253
              +D++ S     F EL            + I +   + LP   S  + E    L+   D
Sbjct: 1447 PAKDVRVSSLVRRFEELSLQLQEKQKRDEELIKARRKRALPVVPSKPVVEVFNDLNEAFD 1506

Query: 1254 NDNFVVSDYEGELS---------SIVACVLALLKDQPFQTDSHNEDS------------- 1313
            ++N   S+ E  ++         S  + V ++ K++  +  S NED+             
Sbjct: 1507 DEN---SEDENGINDTKENRATESNFSGVDSMSKER--ENVSSNEDNSPEAFEDIFGILF 1566

Query: 1314 KGEGGGALQPALSSRSL-----NKVPSNGS------------------------------ 1373
            K E G   Q  L   S      +K+P++G                               
Sbjct: 1567 KNESGLEEQQNLEPSSQMDKEGSKLPTSGPLADKTSVYRILSAFWNEWNSLNPPPFEFPL 1626

Query: 1374 -------SDSDSSISSEE------YRFSSFDRLNLLDSLVSETFKRSDHEGVIKS----- 1433
                   SDS+  I  +E      +  SS D L+ +  +     +   ++G+  S     
Sbjct: 1627 QPTEHMFSDSNVIIREDEPSSLISFTLSSPDYLSKMVEIEDSMDEALTNQGLQGSTQFKI 1686

Query: 1434 ---------------LAKGKYLVNCP--YINQFRDLRSRCCPSELHFIASLSRCINWNAK 1493
                             +G   ++C   +  QF  LR R C  E  F+ SL+RC  W + 
Sbjct: 1687 ENLMLKPTGTHLKYQFEEGSARLSCKVFFAEQFSALR-RACGCEETFVTSLARCSLWESS 1746

Query: 1494 GGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQ 1543
            GGKS S F KT D ++I+K + R E DS + FAP YF YI++ F     T L K+ G Y+
Sbjct: 1747 GGKSGSAFLKTFDKKYILKVLSRLESDSLLNFAPAYFDYISKVFFHELPTALTKIFGFYR 1806

BLAST of Chy1G009970 vs. ExPASy TrEMBL
Match: A0A5D3DIR8 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G001430 PE=4 SV=1)

HSP 1 Score: 2863.2 bits (7421), Expect = 0.0e+00
Identity = 1466/1603 (91.45%), Postives = 1516/1603 (94.57%), Query Frame = 0

Query: 1    MCHCCGAVITDSNGKNLENGSSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60
            MCH CGAVITDSNGKNLENGSS +LDDA  +CLCRVCKENQEQEMMKSDNR+SSEAPTLS
Sbjct: 1    MCHYCGAVITDSNGKNLENGSSLKLDDADPMCLCRVCKENQEQEMMKSDNRNSSEAPTLS 60

Query: 61   PMPSLSSCNS-----------DCSTVANLDVRDGQESATG-SQEDIDCRQERMSRNSSEV 120
            PMPSLSSCNS           DCSTVANLDVRDGQESATG SQEDIDCRQERMS NSSEV
Sbjct: 61   PMPSLSSCNSCVSANYTSFKCDCSTVANLDVRDGQESATGSSQEDIDCRQERMSLNSSEV 120

Query: 121  AHSNDQLSGRDDGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHM 180
            A SNDQLSGRDDGSVMSN+QETTQNASGVPVNAD+EQSNSNWMDPDLWDPPEPEDPEDHM
Sbjct: 121  ADSNDQLSGRDDGSVMSNNQETTQNASGVPVNADVEQSNSNWMDPDLWDPPEPEDPEDHM 180

Query: 181  EGGMGYNDDDDDEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIR 240
            EGGMGYNDDDDDEFGD+SEWST+SSFSRSVDEASVSYRFKEEKQRA+QQVMNGK+KAFIR
Sbjct: 181  EGGMGYNDDDDDEFGDSSEWSTSSSFSRSVDEASVSYRFKEEKQRAMQQVMNGKYKAFIR 240

Query: 241  DLLKL-------GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRN 300
             LLK+       GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDP+A+VKVKCIATGTR 
Sbjct: 241  HLLKVVHVPASSGEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPNAHVKVKCIATGTRK 300

Query: 301  QSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVI 360
            QSQFVKGMVFKKHAAHKHMPTHCKNPKL+LIQGMLGEAPISRLSSFNSMDQENDFTNHVI
Sbjct: 301  QSQFVKGMVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVI 360

Query: 361  EMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMS 420
            EMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMS
Sbjct: 361  EMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMS 420

Query: 421  QKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVK 480
            QKTRQCDAVYFQKIVEEHAGVLEGGKM TKTLMFIEGC TRLGCTILLKGAHSDELKRVK
Sbjct: 421  QKTRQCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVK 480

Query: 481  IVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESA 540
            +VVQCAVVMAF LILETSFIVDQ+AMFATIPFGGVP+ ASSDPQSPLEPRSP VG+AESA
Sbjct: 481  LVVQCAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESA 540

Query: 541  SDKSEQPTSIYISRGSHEEASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVM 600
            SD+ EQPT+I+IS GSHEEAS  SN+E  EKLI+SSEPEPYNPAIFSGFSSISDSLKR M
Sbjct: 541  SDQIEQPTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAM 600

Query: 601  GESFLSSSPYQSLSSYFGHGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQ 660
            GESFL +SPY SLSSYFGH SDLSGL+AKS SIPST QAVYQFD EVRGSSD ENSVHEQ
Sbjct: 601  GESFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQ 660

Query: 661  SVSHQSTLEGLGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIV 720
            SVSHQSTLEGLGFHETAPN + DTMQKK  LDSQSILVLMSSRNALKG MCEQSHFSHIV
Sbjct: 661  SVSHQSTLEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIV 720

Query: 721  FYKHFDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPG 780
            FYK+FDVPLGKFLQ+NLLNQNNLCTVCG LPEAHFY+YAHYRKQLSIQVKQLPGDKVLPG
Sbjct: 721  FYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPG 780

Query: 781  ETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCG 840
            ETEGKLWMWSRCSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSDDTL  KSSVCG
Sbjct: 781  ETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCG 840

Query: 841  HSLFGDFLYFFG--LGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTK 900
            HSLFGDFLYFFG  LGNMVAM RYSEVAIYTVSMPPQKLEFNSSMRQGHLI+E ENVYTK
Sbjct: 841  HSLFGDFLYFFGYVLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTK 900

Query: 901  GVLLFTEIAHSLKKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKG 960
            G+LLF+EIAHSLKKIISER SSNVNHPQ F NDFSFVEEMLNEERSEFE+ IQNSLTKKG
Sbjct: 901  GMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKG 960

Query: 961  NPD-----FLNLNRLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSN 1020
            N D     FLNLNRLLWDLLLESCIWDRRLQSL SLGVTTNSG+SE VEPEP++LKMNSN
Sbjct: 961  NLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSN 1020

Query: 1021 IDVGPEELESTAENDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPSAIEVTEI 1080
            I+VGPEE ES AENDD+K+QQDIS++ENVLPLKEI VEG DGESGGDELNLPSAIEVTEI
Sbjct: 1021 INVGPEESESIAENDDSKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEI 1080

Query: 1081 PIIDDLSPKKLSRQGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKL 1140
             IIDDLSPKKLSRQGTLSNGFD HHSDYEDSQVG+VLSSGDLH+DRTIPISIGDSTLSKL
Sbjct: 1081 QIIDDLSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKL 1140

Query: 1141 FWTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVV 1200
            F TPFSEIRQMHLRDIQRSYFPELKSISSYTPKLL AASDFIHEEGQKLHIHL +DNFVV
Sbjct: 1141 FCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVV 1200

Query: 1201 SDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDS 1260
            SDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDS
Sbjct: 1201 SDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDS 1260

Query: 1261 DSSISSEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRS 1320
            +SSISSEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRS
Sbjct: 1261 ESSISSEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRS 1320

Query: 1321 RCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFK 1380
            RCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFK
Sbjct: 1321 RCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFK 1380

Query: 1381 YINESFDMGNQTCLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGAL 1440
            YINESFDMGNQTCLAKVLGIYQVTVRE KSGKEMRHDLMVMENLSFGRNIIRQYDLKGAL
Sbjct: 1381 YINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGAL 1440

Query: 1441 HARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSL 1500
            HARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKR LQRAIWNDT FLNSINVMDYSL
Sbjct: 1441 HARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSL 1500

Query: 1501 LVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMS 1560
            LVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTV+SPKEYKRRFRKFMS
Sbjct: 1501 LVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMS 1560

Query: 1561 AHFLSVPDHWCMRQNLPGPWDLFDTADPASSHRTNADENDVSS 1578
            AHFLSVPDHWCM+QNLPGPWDL  TAD ASS++TNA+EN +SS
Sbjct: 1561 AHFLSVPDHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1603

BLAST of Chy1G009970 vs. ExPASy TrEMBL
Match: A0A0A0K755 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucumis sativus OX=3659 GN=Csa_7G206930 PE=4 SV=1)

HSP 1 Score: 2848.5 bits (7383), Expect = 0.0e+00
Identity = 1439/1477 (97.43%), Postives = 1453/1477 (98.38%), Query Frame = 0

Query: 101  MSRNSSEVAHSNDQLSGRDDGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPE 160
            MSRNSSEVAHSNDQLSGRDDGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPE
Sbjct: 1    MSRNSSEVAHSNDQLSGRDDGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPE 60

Query: 161  PEDPEDHMEGGMGYNDDDDDEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMN 220
            PEDPEDHMEGGMGYNDDDD+EFGDNSEWSTASSFSRSVDE SVSYRFKEEKQRALQQVMN
Sbjct: 61   PEDPEDHMEGGMGYNDDDDEEFGDNSEWSTASSFSRSVDETSVSYRFKEEKQRALQQVMN 120

Query: 221  GKHKAFIRDLLKLGEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTR 280
            GKHKAFIRDLLKLGEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTR
Sbjct: 121  GKHKAFIRDLLKLGEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTR 180

Query: 281  NQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHV 340
            NQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHV
Sbjct: 181  NQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHV 240

Query: 341  IEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLM 400
            IEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLM
Sbjct: 241  IEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLM 300

Query: 401  SQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRV 460
            SQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGC TRLGCTILLKGAHSDELKRV
Sbjct: 301  SQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCPTRLGCTILLKGAHSDELKRV 360

Query: 461  KIVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAES 520
            KIVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSD QSPLEPRSPNVGKAES
Sbjct: 361  KIVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSDRQSPLEPRSPNVGKAES 420

Query: 521  ASDKSEQPTSIYISRGSHEEASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRV 580
            ASDKSEQPT+I ISRGSHEE STGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRV
Sbjct: 421  ASDKSEQPTNICISRGSHEETSTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRV 480

Query: 581  MGESFLSSSPYQSLSSYFGHGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHE 640
            MGESFLSSSPYQSLSSYFGHGSDLSGL+AKS SIPSTPQAVYQFDVEV+GSSDEENSVHE
Sbjct: 481  MGESFLSSSPYQSLSSYFGHGSDLSGLVAKSGSIPSTPQAVYQFDVEVKGSSDEENSVHE 540

Query: 641  QSVSHQSTLEGLGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHI 700
             SVSHQSTLEGLGFHETA NYSVDTMQKKMSLDSQSILVLMSSRNALKGA+CEQSHFSHI
Sbjct: 541  SSVSHQSTLEGLGFHETALNYSVDTMQKKMSLDSQSILVLMSSRNALKGAICEQSHFSHI 600

Query: 701  VFYKHFDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLP 760
            VFYKHFDVPLGKFLQ+NLLNQNNLCTVCG LPEAH YF+AHYRKQLSIQVKQLPGDKVLP
Sbjct: 601  VFYKHFDVPLGKFLQENLLNQNNLCTVCGELPEAHIYFFAHYRKQLSIQVKQLPGDKVLP 660

Query: 761  GETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVC 820
            GETEGKLWMWSRCSKCKSTGGPSKSTKRV ISTAARGLSFGKFLELCFSDDTLL KS VC
Sbjct: 661  GETEGKLWMWSRCSKCKSTGGPSKSTKRVPISTAARGLSFGKFLELCFSDDTLLSKSPVC 720

Query: 821  GHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKG 880
            GHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGH IRETENVYTKG
Sbjct: 721  GHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHFIRETENVYTKG 780

Query: 881  VLLFTEIAHSLKKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKGN 940
            VLLFTEIAHSLKKIISERKSSNVNHPQ FPNDFSFVEEMLNEERSEFE+NIQNSLTKKGN
Sbjct: 781  VLLFTEIAHSLKKIISERKSSNVNHPQGFPNDFSFVEEMLNEERSEFEINIQNSLTKKGN 840

Query: 941  PDFLNLNRLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPE 1000
            PDFLNLNRLLWDLLLESCIWDRRLQSL SLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPE
Sbjct: 841  PDFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPE 900

Query: 1001 ELESTAENDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIIDDL 1060
            ELES AEND+TKVQQDISL+ENVLPLKEITVEG DGESGGDELNLPSAIEVTEIPIIDD 
Sbjct: 901  ELESIAENDETKVQQDISLDENVLPLKEITVEGSDGESGGDELNLPSAIEVTEIPIIDDP 960

Query: 1061 SPKKLSRQGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFS 1120
            SPKKLSRQGTLSNGFDYHHSDYEDSQVG+VLSSGDLHIDRTIPISIGDSTLSKLF TPFS
Sbjct: 961  SPKKLSRQGTLSNGFDYHHSDYEDSQVGRVLSSGDLHIDRTIPISIGDSTLSKLFCTPFS 1020

Query: 1121 EIRQMHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGE 1180
            EIRQMHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHL NDNFVVSDYEGE
Sbjct: 1021 EIRQMHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLANDNFVVSDYEGE 1080

Query: 1181 LSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISS 1240
            LSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISS
Sbjct: 1081 LSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISS 1140

Query: 1241 EEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSE 1300
            EEYRFSSFDRLNLLDSLVSETFKRSD+EGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSE
Sbjct: 1141 EEYRFSSFDRLNLLDSLVSETFKRSDYEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSE 1200

Query: 1301 LHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESF 1360
            LHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESF
Sbjct: 1201 LHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESF 1260

Query: 1361 DMGNQTCLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNP 1420
            DMGNQTCLAKV+GIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNP
Sbjct: 1261 DMGNQTCLAKVVGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNP 1320

Query: 1421 ATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDA 1480
            ATNNFGEVLLDQNFVNDMNSSPLYVSNRA R LQRAIWNDTAFLNSINVMDYSLLVGVDA
Sbjct: 1321 ATNNFGEVLLDQNFVNDMNSSPLYVSNRAMRRLQRAIWNDTAFLNSINVMDYSLLVGVDA 1380

Query: 1481 EKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSV 1540
            EKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSV
Sbjct: 1381 EKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSV 1440

Query: 1541 PDHWCMRQNLPGPWDLFDTADPASSHRTNADENDVSS 1578
            PDHWCM+QNLPGPWDLFDTADPASSHRTNADEND SS
Sbjct: 1441 PDHWCMQQNLPGPWDLFDTADPASSHRTNADENDASS 1477

BLAST of Chy1G009970 vs. ExPASy TrEMBL
Match: A0A6J1EWK7 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111439025 PE=4 SV=1)

HSP 1 Score: 2442.2 bits (6328), Expect = 0.0e+00
Identity = 1259/1590 (79.18%), Postives = 1367/1590 (85.97%), Query Frame = 0

Query: 1    MCHCCGAVITDSNGKNLENGSSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60
            MCH CGAVITDS GK L NG+S  LDD+GTVC+CRVCKENQE + M  DN+SSSEA  LS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCNSDCSTVANLDVRDGQESAT-GSQEDIDCRQERMSRNSSEVAHSNDQLSGRD 120
            PMPSLSSC+SDCSTVANLDV+DGQ+  T  SQ+ IDCRQERMS+N SEV HSN QLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDD 180
            D SV++ SQE T N SGV VNA++EQS+SNW+D DLWDPPEPEDP+DHMEGGMGY+DDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHMEGGMGYDDDDD 180

Query: 181  DEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIRDLLKL------ 240
            DEF D++EW+T+SSFSRSVDEASVSYR KE+KQRA+QQ+MNGK K FI  LLK       
Sbjct: 181  DEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240

Query: 241  GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFKK 300
            GEDSENWVDIVSSLSWEAATFLKPVVNGKA+DP+A+VKVKCIATGTR+QSQFVKGMVFKK
Sbjct: 241  GEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300

Query: 301  HAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVIL 360
            HAAHKHMPTHCKNPKLILI+GMLGEAPISRLSSFNSMDQENDFT HVIEMIE CTANVIL
Sbjct: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360

Query: 361  VEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDAVYFQ 420
            VEKT +RVIQEA+LKKGMTLVLDMKLHRL+RIALCTGSPILTSETLMSQ TRQCDAVYF+
Sbjct: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420

Query: 421  KIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQCAVVMAFH 480
            KIVEEHAG+LEGGK PTKTLMFIEGC TRLGCTILLKGAHSDELK++K+VVQ AVVMAFH
Sbjct: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480

Query: 481  LILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESASDKSEQPTSIYI 540
            L+LETSF+VDQRAMFATIPFGGVPAT SSDPQSPLEP SPN                   
Sbjct: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNA------------------ 540

Query: 541  SRGSHEEASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQS 600
                 + AS GSN+          E EPYNPAIFSG SSISDSLKRVMGESF  +SP QS
Sbjct: 541  -----QGASNGSNL----------EGEPYNPAIFSGLSSISDSLKRVMGESFFLASPCQS 600

Query: 601  LSSYFGHGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSHQSTL-EGL 660
             SSYFGHG DLSGL+AKS SIPSTP    QFDVEVRGSSDEENS+HEQ V HQST  EG 
Sbjct: 601  FSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDVEVRGSSDEENSIHEQFVPHQSTFDEGF 660

Query: 661  GFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLGK 720
            GFHETAPNYS D + +K   +SQSILVLMSSRNA KG MCEQSHFSHIVFYK+FD PLGK
Sbjct: 661  GFHETAPNYSEDKLPEKTPFNSQSILVLMSSRNASKGTMCEQSHFSHIVFYKNFDAPLGK 720

Query: 721  FLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSR 780
            FLQ+NLLNQ N+CTVCG LPEAH+Y+YAH RKQL IQVKQLP +KVLPGETEGKLWMWSR
Sbjct: 721  FLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVNKVLPGETEGKLWMWSR 780

Query: 781  CSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYFF 840
            CSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSD TL  KSSVCGHSLFGDFLYFF
Sbjct: 781  CSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSLFGDFLYFF 840

Query: 841  GLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSLK 900
            GLGNMVAM RY+EVAIYTVSMPPQKLEFNSSMRQGHLI ETENVY KG++LFTEIA  LK
Sbjct: 841  GLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITETENVYAKGMILFTEIAQFLK 900

Query: 901  KIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKGNPDF-----LNLN 960
            KIISERKSS V       NDFS +EEMLNEERSEFELNIQ+SLTKKGNPDF     L+LN
Sbjct: 901  KIISERKSSTV-------NDFSLLEEMLNEERSEFELNIQSSLTKKGNPDFPFHKYLSLN 960

Query: 961  RLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELESTAE 1020
            RLLW+LLLESCIWD+RLQ L S+GVT NSG+SE VEPEP          VG E LES A+
Sbjct: 961  RLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP----------VGQEGLESIAQ 1020

Query: 1021 NDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIIDDLSPKKLSR 1080
             DD  VQQD+S++EN L  KEI VE LDGES GDEL LPSA EVTEIPI+DDLSPK+LSR
Sbjct: 1021 TDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPIMDDLSPKQLSR 1080

Query: 1081 QGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMHL 1140
            QG+LSNGF+  HSD ED Q  +VLSSGD      IP S G+ TL KLFWTPFSEI+QM +
Sbjct: 1081 QGSLSNGFNRRHSDDEDPQAVRVLSSGD------IPSSTGNLTLDKLFWTPFSEIQQMRM 1140

Query: 1141 RDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSIVAC 1200
            RDIQRSYFPELKS+S+YTPKLLP A DFI++EGQKLHI L N+ F+VSDYEGELSSIVAC
Sbjct: 1141 RDIQRSYFPELKSVSNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELSSIVAC 1200

Query: 1201 VLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFSS 1260
            VLALLKD   +TD HNEDSKGEG            LNKVPSNGSSDSDSS+SSEE++FSS
Sbjct: 1201 VLALLKDLSLETDLHNEDSKGEG------------LNKVPSNGSSDSDSSVSSEEHQFSS 1260

Query: 1261 FDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASL 1320
            FDRLNLLDSL+ +TFKR++HEGVIKSLAKGKYLVNCPY NQFRDLR RCCPSELH+IASL
Sbjct: 1261 FDRLNLLDSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASL 1320

Query: 1321 SRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTC 1380
            SRCI+WNAKGGKSKSFFAKTLDDRFIIKEIKRTEY+SFMKFAP+YFKYINESFDMGNQTC
Sbjct: 1321 SRCIHWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTC 1380

Query: 1381 LAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGE 1440
            LAKVLGIYQVTVREPKSGKE+RH+LMVMENLSFGRNIIRQYDLKGALHAR+NPATN+FGE
Sbjct: 1381 LAKVLGIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGE 1440

Query: 1441 VLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVC 1500
            VLLDQNFVNDMNSSPLYV+N+AKR LQRAIWNDTAFLNS+NVMDYSLLVGVDAEKKELVC
Sbjct: 1441 VLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVC 1500

Query: 1501 GIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWCMR 1560
            GIIDYLRQYTWDKQLETW KSSL PKNVLPTV+SPKEYKRRFRKFMSAHFLSVPD+WC +
Sbjct: 1501 GIIDYLRQYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQ 1522

Query: 1561 QNLPGPWDLFDTADPASSHRTNADENDVSS 1578
            QNL GPW         +S +TNA+END+SS
Sbjct: 1561 QNLHGPWGACGGTIDRASSQTNAEENDLSS 1522

BLAST of Chy1G009970 vs. ExPASy TrEMBL
Match: A0A6J1IGZ0 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita maxima OX=3661 GN=LOC111477135 PE=4 SV=1)

HSP 1 Score: 2433.7 bits (6306), Expect = 0.0e+00
Identity = 1255/1590 (78.93%), Postives = 1364/1590 (85.79%), Query Frame = 0

Query: 1    MCHCCGAVITDSNGKNLENGSSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60
            MCH CGAVITDS GK L NG+S  LDD+GTVC+CRVCKENQE E M  DN+SSSEA  LS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELEAMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCNSDCSTVANLDVRDGQESAT-GSQEDIDCRQERMSRNSSEVAHSNDQLSGRD 120
            PMPSLSSC+SDCSTVANLD++DGQE  T  SQ+ IDCRQERMS+N SEV HSN QLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDD 180
            D SV++ SQE T N SGV VNA++EQS+SNW+D DLWDPPEPEDP+DH+EGGMGY+DDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  DEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIRDLLKL------ 240
            DEF D++EW+T+SSFSRSVDEAS+SYR KE+KQRA+QQ+MNGK K FI  LLK       
Sbjct: 181  DEFDDSTEWNTSSSFSRSVDEASISYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240

Query: 241  GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFKK 300
            GE SENWVDIVSSLSWEAATFLKPVVNGKA+DP+A+VKVKCIATGTR+QSQFVKGMVFKK
Sbjct: 241  GEGSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300

Query: 301  HAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVIL 360
            HAAHKHMPTHCKNPKLILI+GMLGEAPISRLSSFNSMDQENDFT HVIEMIE CTANVIL
Sbjct: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360

Query: 361  VEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDAVYFQ 420
            VEKT +RVIQEA+LKKGMTLVLDMKLHRL+RIALCTGSPILTSETLMSQ TRQCDAVYF+
Sbjct: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420

Query: 421  KIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQCAVVMAFH 480
            KIVEEHAG+LEGGK PTKTLMFIEGC TRLGCTILLKGAHSDELK++K+VVQ AVVMAFH
Sbjct: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480

Query: 481  LILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESASDKSEQPTSIYI 540
            L+LETSF+VDQRAMFATIPFGGVPAT SSDPQSPLEP SPN                   
Sbjct: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNA------------------ 540

Query: 541  SRGSHEEASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQS 600
                 + AS GSN+          E EPYN AIFSG SSISDSLKRVMGESF  +SP QS
Sbjct: 541  -----QGASNGSNL----------EGEPYNAAIFSGLSSISDSLKRVMGESFFLASPCQS 600

Query: 601  LSSYFGHGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSHQSTL-EGL 660
             SSYFGHG DLSGL+AKS SIPSTP    QFDVEVRGSSDEENS+HEQ V HQST  EG 
Sbjct: 601  FSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDVEVRGSSDEENSIHEQFVPHQSTFDEGF 660

Query: 661  GFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLGK 720
            GFHETAPNYS D + KK   +SQSILVLMSSRNALKG MCEQSHFSHIVFYK+FD PLGK
Sbjct: 661  GFHETAPNYSEDKLPKKTPFNSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDAPLGK 720

Query: 721  FLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSR 780
            FLQ+NLLNQ N+CTVCG LPEAH+Y+YAH RKQL IQVKQLP DKVLPGETEGKLWMWSR
Sbjct: 721  FLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVDKVLPGETEGKLWMWSR 780

Query: 781  CSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYFF 840
            CSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSD TL  KSSVCGHSLFGDFLYFF
Sbjct: 781  CSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSLFGDFLYFF 840

Query: 841  GLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSLK 900
            GLGNMVAM RY+EVAIYTVSMPPQKLEFN+SMRQGHLI ETENVY KG++LFTEIA  LK
Sbjct: 841  GLGNMVAMFRYTEVAIYTVSMPPQKLEFNNSMRQGHLITETENVYEKGMILFTEIAQFLK 900

Query: 901  KIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKGNPDF-----LNLN 960
            KIISERKSS V       NDFS +EEMLNEERSEFELNIQ+SLTKKGNPDF     L+LN
Sbjct: 901  KIISERKSSTV-------NDFSLLEEMLNEERSEFELNIQSSLTKKGNPDFPFHKYLSLN 960

Query: 961  RLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELESTAE 1020
            RLLW+LLLESCIWD+RLQ L SLGVT NSG+SE VEPEP          V  E LES A+
Sbjct: 961  RLLWELLLESCIWDKRLQLLTSLGVTINSGTSENVEPEP----------VRHEGLESIAQ 1020

Query: 1021 NDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIIDDLSPKKLSR 1080
             DD  VQQD+S++EN L  KEI VE LDGES GDEL LPSA EVTEIPI+DDLSPK+LSR
Sbjct: 1021 TDDRNVQQDVSVDENALHSKEIAVEHLDGESDGDELGLPSATEVTEIPIMDDLSPKQLSR 1080

Query: 1081 QGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMHL 1140
            QG+L NGF+  HSD ED Q G+VLSSGD      IP S G+ T  KLFWTPFSEI+QM +
Sbjct: 1081 QGSLLNGFNRRHSDDEDPQAGRVLSSGD------IPSSTGNLTSDKLFWTPFSEIQQMRM 1140

Query: 1141 RDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSIVAC 1200
            RDIQRSYFPELKSIS+YTPKLLP A +FI++EGQKLHI L N+ F+VSDYEGELSSIVAC
Sbjct: 1141 RDIQRSYFPELKSISNYTPKLLPTAHEFINKEGQKLHIFLSNEKFIVSDYEGELSSIVAC 1200

Query: 1201 VLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFSS 1260
            VLALLKD   +TD HNEDSKGEG            LNKVPS+GSSDSDSS++SEE++FSS
Sbjct: 1201 VLALLKDLSLETDLHNEDSKGEG------------LNKVPSSGSSDSDSSVTSEEHQFSS 1260

Query: 1261 FDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASL 1320
            FDRLNLLDSL+ +TFKR++HEGVIKSLAKGKYLVNCPY NQFRDLR RCCPSELH+IASL
Sbjct: 1261 FDRLNLLDSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASL 1320

Query: 1321 SRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTC 1380
            SRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEY+SFMKFAP+YFKYINESFDMGNQTC
Sbjct: 1321 SRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTC 1380

Query: 1381 LAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGE 1440
            LAKVLGIYQVTVREPKSGKE+RH+LMVMENLSFGRNIIRQYDLKGALHAR+NPATN+FGE
Sbjct: 1381 LAKVLGIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGE 1440

Query: 1441 VLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVC 1500
            VLLDQNFVNDMNSSPLYV+N+AKR LQRAIWNDTAFLNS+NVMDYSLLVGVDA+KKELVC
Sbjct: 1441 VLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAKKKELVC 1500

Query: 1501 GIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWCMR 1560
            GIIDYLRQYTWDKQLETW KSSL PKNVLPTV+SPKEYKRRFRKFMSAHFLSVPD+WC +
Sbjct: 1501 GIIDYLRQYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQ 1522

Query: 1561 QNLPGPWDLFDTADPASSHRTNADENDVSS 1578
            QNL GPW         +S +TNA+END+SS
Sbjct: 1561 QNLHGPWGACGGTIDHASSQTNAEENDLSS 1522

BLAST of Chy1G009970 vs. ExPASy TrEMBL
Match: A0A6J1F0I5 (1-phosphatidylinositol-3-phosphate 5-kinase OS=Cucurbita moschata OX=3662 GN=LOC111438379 PE=4 SV=1)

HSP 1 Score: 2391.3 bits (6196), Expect = 0.0e+00
Identity = 1237/1566 (78.99%), Postives = 1353/1566 (86.40%), Query Frame = 0

Query: 1    MCHCCGAVITDSNGKNLENGSSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60
            MCH CGAV+ DS  + LENGSS +LDDAG +C C+VC ENQEQE MK D+ SSSE+  LS
Sbjct: 1    MCHHCGAVVADSAAQKLENGSSVKLDDAGAMCFCKVCNENQEQETMKRDDGSSSESLMLS 60

Query: 61   PMPSLSSCNSDCSTVANLDVRDGQESATG-SQEDIDCRQERMSRNSSEVAHSNDQLSGRD 120
            PMPS S+CNSDCST+ +LD+R+GQE +TG SQEDID +QER S+N S V HSN QL GRD
Sbjct: 61   PMPSPSTCNSDCSTMGSLDIRNGQEGSTGSSQEDIDHKQERRSQNLSGVVHSNVQLGGRD 120

Query: 121  DGSVMS-NSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEGGMGY---N 180
            D SVM  N+QE T N  GVPVNA+ E+SNS+WMD +LWDPPEPEDP+DHMEGGMGY   +
Sbjct: 121  DESVMPINNQEATHNGFGVPVNAEFEKSNSSWMDTNLWDPPEPEDPDDHMEGGMGYYDDD 180

Query: 181  DDDDDEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIRDLLKL-- 240
            DDDDDEFGD++EWS +SSFSRSVDEASVS+R KEEKQRA+Q+VMNGK+KAFI  LL L  
Sbjct: 181  DDDDDEFGDSTEWSMSSSFSRSVDEASVSHRSKEEKQRAMQEVMNGKYKAFIHHLLNLVG 240

Query: 241  ----GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFVKGM 300
                GED ENW DIV SLSWEAATFLKP+VNGKAMDP A+VKVKCIA+GTR+QSQFVKGM
Sbjct: 241  VPSSGEDGENWADIVCSLSWEAATFLKPLVNGKAMDPGAHVKVKCIASGTRSQSQFVKGM 300

Query: 301  VFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTA 360
            VFKKHAAHKHM T+ KNP+LILIQG LGEAPIS LSSFNSMDQENDFTN+VIEMIE CTA
Sbjct: 301  VFKKHAAHKHMVTNWKNPRLILIQGRLGEAPISGLSSFNSMDQENDFTNYVIEMIETCTA 360

Query: 361  NVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDA 420
            NVI+VEKTA+RVIQEAILK+ +TLVLDMKLHRL+RIALCTGSPILTSETL SQK RQCDA
Sbjct: 361  NVIIVEKTASRVIQEAILKRCITLVLDMKLHRLERIALCTGSPILTSETLASQKARQCDA 420

Query: 421  VYFQKIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQCAVV 480
            VYFQK+V EH+G LEGGK  TKTLMFIEGC  RLGCTILLKGA S ELKRVK+VVQ AVV
Sbjct: 421  VYFQKVV-EHSGGLEGGKRSTKTLMFIEGCPARLGCTILLKGARS-ELKRVKLVVQRAVV 480

Query: 481  MAFHLILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESASDKSEQ-- 540
            MAFHLILETSF+VDQRAMFATIPFGGV    S DPQSPLEP S N+ + E A+DKSE   
Sbjct: 481  MAFHLILETSFLVDQRAMFATIPFGGVSTMVSPDPQSPLEPCSSNILQVEKANDKSEPCV 540

Query: 541  --PTSIYISRGSHEEASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESF 600
               T IYIS G HEE+S GSN++  EK I+ SE EPYNPAI SGFSSISDS K+VMGESF
Sbjct: 541  SLLTDIYISSGPHEESSNGSNLKPVEKPIIPSELEPYNPAILSGFSSISDSSKKVMGESF 600

Query: 601  LSSSPYQSLSSYFGHGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSH 660
            L SS + SLSSYFGH  D  GL  KS SIPSTPQA  +FDV+VRGSSDEENS HEQS SH
Sbjct: 601  LLSSTHHSLSSYFGHERDWIGLAPKSDSIPSTPQAANRFDVKVRGSSDEENSGHEQSASH 660

Query: 661  QSTLEGLGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKH 720
            QS LEGLGFHE APN S +TMQKK SLDSQSILVLMSSRNALKG MCEQSHF HIVFY++
Sbjct: 661  QSALEGLGFHENAPNCSEETMQKKSSLDSQSILVLMSSRNALKGTMCEQSHFLHIVFYRN 720

Query: 721  FDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEG 780
            FDVPLGKFLQ+NLLNQ N CTVC  LPEAHFY++AHY KQLSIQV+QLPG K+LPG+TEG
Sbjct: 721  FDVPLGKFLQENLLNQTNFCTVCSELPEAHFYYFAHYGKQLSIQVQQLPGYKLLPGQTEG 780

Query: 781  KLWMWSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLF 840
            KLWMWSRCSKCKS G PS STK VLISTAAR LSFGKFLELCFSD+TL  KSS CGHSLF
Sbjct: 781  KLWMWSRCSKCKSKGEPSTSTKMVLISTAARSLSFGKFLELCFSDETLPSKSSGCGHSLF 840

Query: 841  GDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFT 900
            GDFLYFFGLGN VAM RYS  AIYTVSMPPQKL+FNSSMRQGHLIRETENV  KG LLFT
Sbjct: 841  GDFLYFFGLGNRVAMFRYSAAAIYTVSMPPQKLDFNSSMRQGHLIRETENVKKKGKLLFT 900

Query: 901  EIAHSLKKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKGNPDFLN 960
            EIAHSLKKI SE +SSNV  PQCF NDFS +EEMLN+ERSEFE+ IQNSL       +L+
Sbjct: 901  EIAHSLKKISSECRSSNVK-PQCFLNDFSSIEEMLNQERSEFEVKIQNSLAFH---KYLS 960

Query: 961  LNRLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELEST 1020
            LN+LLW+LLLESCIWDRRLQSL SLG+T+  G+SE VEPE + + MNSNID   EE ES 
Sbjct: 961  LNQLLWELLLESCIWDRRLQSLTSLGLTSTPGTSENVEPESVTMNMNSNIDARHEESESI 1020

Query: 1021 AENDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIIDDLSPKKL 1080
            A NDDT+VQQDIS +ENVLP+KEI VEGLDGESG DE + PS  EVTEI I+DDLSPK L
Sbjct: 1021 AANDDTEVQQDISWDENVLPMKEIAVEGLDGESGCDENHSPSVTEVTEIRIMDDLSPKHL 1080

Query: 1081 SRQGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQM 1140
            SRQG+LSNGF+ HH D EDSQV +V SSGD+H+DRTIP+   DS+L KL W P  EIRQM
Sbjct: 1081 SRQGSLSNGFNQHHLDDEDSQV-RVASSGDMHVDRTIPVLTADSSLGKLLWAPCVEIRQM 1140

Query: 1141 HLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSIV 1200
            H+RDIQRS+FPELKS SSYTPKL+PAAS F +EEGQKLHI L  DNF+VSDYEGELSSIV
Sbjct: 1141 HIRDIQRSFFPELKSFSSYTPKLIPAASAFTNEEGQKLHIRLAKDNFIVSDYEGELSSIV 1200

Query: 1201 ACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEYRF 1260
            ACVLALL+D   QTDSHNEDSKGEGGG  QP LSS+SLNKVPSNGSSDSDSS +S+ Y+F
Sbjct: 1201 ACVLALLEDLSLQTDSHNEDSKGEGGGVSQPNLSSQSLNKVPSNGSSDSDSSAASDAYQF 1260

Query: 1261 SSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIA 1320
            S+F+RLNLLDSLV ETFKR+DHEGVIKSLA GKY+VNCPY NQFRDLRS+CCPSELH+IA
Sbjct: 1261 SNFERLNLLDSLVPETFKRTDHEGVIKSLANGKYVVNCPYFNQFRDLRSQCCPSELHYIA 1320

Query: 1321 SLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQ 1380
            SLSRCI WNAKGGKS SFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYI +SFDMGNQ
Sbjct: 1321 SLSRCIKWNAKGGKSNSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYIKQSFDMGNQ 1380

Query: 1381 TCLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNF 1440
            TCLAKVLGIYQV VREPKSGKEMRHD+MVMENLSFGRNIIRQYDLKGALHAR+NPA NNF
Sbjct: 1381 TCLAKVLGIYQVAVREPKSGKEMRHDVMVMENLSFGRNIIRQYDLKGALHARYNPAANNF 1440

Query: 1441 GEVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKEL 1500
            GEVLLDQNFV DMNSSP+YVSNRAKR +QRAIWNDTAFLNSINVMDYSLLVGVDAEKKE 
Sbjct: 1441 GEVLLDQNFVKDMNSSPIYVSNRAKRRMQRAIWNDTAFLNSINVMDYSLLVGVDAEKKEF 1500

Query: 1501 VCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWC 1552
            VCGIIDYLRQYTWDKQLETWVKSSL+PKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWC
Sbjct: 1501 VCGIIDYLRQYTWDKQLETWVKSSLVPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWC 1559

BLAST of Chy1G009970 vs. NCBI nr
Match: XP_011658963.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucumis sativus] >KAE8646042.1 hypothetical protein Csa_016172 [Cucumis sativus])

HSP 1 Score: 3021 bits (7833), Expect = 0.0
Identity = 1537/1578 (97.40%), Postives = 1552/1578 (98.35%), Query Frame = 0

Query: 1    MCHCCGAVITDSNGKNLENGSSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60
            MCHCCGA ITDSNGKNLENG+SHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS
Sbjct: 1    MCHCCGAAITDSNGKNLENGTSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60

Query: 61   PMPSLSSCNSDCSTVANLDVRDGQESATGS-QEDIDCRQERMSRNSSEVAHSNDQLSGRD 120
            PMPSLSSCNSDCSTVANLDVRDGQESATGS QEDIDCRQERMSRNSSEVAHSNDQLSGRD
Sbjct: 61   PMPSLSSCNSDCSTVANLDVRDGQESATGSSQEDIDCRQERMSRNSSEVAHSNDQLSGRD 120

Query: 121  DGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDD 180
            DGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDD
Sbjct: 121  DGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDD 180

Query: 181  DEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIRDLLKLGEDSEN 240
            +EFGDNSEWSTASSFSRSVDE SVSYRFKEEKQRALQQVMNGKHKAFIRDLLKLGEDSEN
Sbjct: 181  EEFGDNSEWSTASSFSRSVDETSVSYRFKEEKQRALQQVMNGKHKAFIRDLLKLGEDSEN 240

Query: 241  WVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFKKHAAHKH 300
            WVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFKKHAAHKH
Sbjct: 241  WVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFKKHAAHKH 300

Query: 301  MPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVILVEKTAA 360
            MPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVILVEKTAA
Sbjct: 301  MPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVILVEKTAA 360

Query: 361  RVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDAVYFQKIVEEH 420
            RVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDAVYFQKIVEEH
Sbjct: 361  RVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDAVYFQKIVEEH 420

Query: 421  AGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETS 480
            AGVLEGGKMPTKTLMFIEGC TRLGCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETS
Sbjct: 421  AGVLEGGKMPTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETS 480

Query: 481  FIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESASDKSEQPTSIYISRGSHE 540
            FIVDQRAMFATIPFGGVPATASSD QSPLEPRSPNVGKAESASDKSEQPT+I ISRGSHE
Sbjct: 481  FIVDQRAMFATIPFGGVPATASSDRQSPLEPRSPNVGKAESASDKSEQPTNICISRGSHE 540

Query: 541  EASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQSLSSYFG 600
            E STGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQSLSSYFG
Sbjct: 541  ETSTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQSLSSYFG 600

Query: 601  HGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSHQSTLEGLGFHETAP 660
            HGSDLSGL+AKS SIPSTPQAVYQFDVEV+GSSDEENSVHE SVSHQSTLEGLGFHETA 
Sbjct: 601  HGSDLSGLVAKSGSIPSTPQAVYQFDVEVKGSSDEENSVHESSVSHQSTLEGLGFHETAL 660

Query: 661  NYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLGKFLQDNLL 720
            NYSVDTMQKKMSLDSQSILVLMSSRNALKGA+CEQSHFSHIVFYKHFDVPLGKFLQ+NLL
Sbjct: 661  NYSVDTMQKKMSLDSQSILVLMSSRNALKGAICEQSHFSHIVFYKHFDVPLGKFLQENLL 720

Query: 721  NQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKST 780
            NQNNLCTVCG LPEAH YF+AHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKST
Sbjct: 721  NQNNLCTVCGELPEAHIYFFAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKST 780

Query: 781  GGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYFFGLGNMVA 840
            GGPSKSTKRV ISTAARGLSFGKFLELCFSDDTLL KS VCGHSLFGDFLYFFGLGNMVA
Sbjct: 781  GGPSKSTKRVPISTAARGLSFGKFLELCFSDDTLLSKSPVCGHSLFGDFLYFFGLGNMVA 840

Query: 841  MSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSLKKIISERK 900
            MSRYSEVAIYTVSMPPQKLEFNSSMRQGH IRETENVYTKGVLLFTEIAHSLKKIISERK
Sbjct: 841  MSRYSEVAIYTVSMPPQKLEFNSSMRQGHFIRETENVYTKGVLLFTEIAHSLKKIISERK 900

Query: 901  SSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKGNPDFLNLNRLLWDLLLESCI 960
            SSNVNHPQ FPNDFSFVEEMLNEERSEFE+NIQNSLTKKGNPDFLNLNRLLWDLLLESCI
Sbjct: 901  SSNVNHPQGFPNDFSFVEEMLNEERSEFEINIQNSLTKKGNPDFLNLNRLLWDLLLESCI 960

Query: 961  WDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELESTAENDDTKVQQDISL 1020
            WDRRLQSL SLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELES AEND+TKVQQDISL
Sbjct: 961  WDRRLQSLASLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELESIAENDETKVQQDISL 1020

Query: 1021 EENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIIDDLSPKKLSRQGTLSNGFDYHH 1080
            +ENVLPLKEITVEG DGESGGDELNLPSAIEVTEIPIIDD SPKKLSRQGTLSNGFDYHH
Sbjct: 1021 DENVLPLKEITVEGSDGESGGDELNLPSAIEVTEIPIIDDPSPKKLSRQGTLSNGFDYHH 1080

Query: 1081 SDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMHLRDIQRSYFPELK 1140
            SDYEDSQVG+VLSSGDLHIDRTIPISIGDSTLSKLF TPFSEIRQMHLRDIQRSYFPELK
Sbjct: 1081 SDYEDSQVGRVLSSGDLHIDRTIPISIGDSTLSKLFCTPFSEIRQMHLRDIQRSYFPELK 1140

Query: 1141 SISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSIVACVLALLKDQPFQT 1200
            SISSYTPKLLPAASDFIHEEGQKLHIHL NDNFVVSDYEGELSSIVACVLALLKDQPFQT
Sbjct: 1141 SISSYTPKLLPAASDFIHEEGQKLHIHLANDNFVVSDYEGELSSIVACVLALLKDQPFQT 1200

Query: 1201 DSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFSSFDRLNLLDSLVS 1260
            DSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFSSFDRLNLLDSLVS
Sbjct: 1201 DSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFSSFDRLNLLDSLVS 1260

Query: 1261 ETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGK 1320
            ETFKRSD+EGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGK
Sbjct: 1261 ETFKRSDYEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGK 1320

Query: 1321 SKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTV 1380
            SKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKV+GIYQVTV
Sbjct: 1321 SKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVVGIYQVTV 1380

Query: 1381 REPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMN 1440
            REPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMN
Sbjct: 1381 REPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMN 1440

Query: 1441 SSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWD 1500
            SSPLYVSNRA R LQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWD
Sbjct: 1441 SSPLYVSNRAMRRLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWD 1500

Query: 1501 KQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWCMRQNLPGPWDLFDT 1560
            KQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWCM+QNLPGPWDLFDT
Sbjct: 1501 KQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWCMQQNLPGPWDLFDT 1560

Query: 1561 ADPASSHRTNADENDVSS 1577
            ADPASSHRTNADEND SS
Sbjct: 1561 ADPASSHRTNADENDASS 1578

BLAST of Chy1G009970 vs. NCBI nr
Match: TYK23139.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucumis melo var. makuwa])

HSP 1 Score: 2845 bits (7374), Expect = 0.0
Identity = 1466/1603 (91.45%), Postives = 1516/1603 (94.57%), Query Frame = 0

Query: 1    MCHCCGAVITDSNGKNLENGSSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60
            MCH CGAVITDSNGKNLENGSS +LDDA  +CLCRVCKENQEQEMMKSDNR+SSEAPTLS
Sbjct: 1    MCHYCGAVITDSNGKNLENGSSLKLDDADPMCLCRVCKENQEQEMMKSDNRNSSEAPTLS 60

Query: 61   PMPSLSSCNS-----------DCSTVANLDVRDGQESATGS-QEDIDCRQERMSRNSSEV 120
            PMPSLSSCNS           DCSTVANLDVRDGQESATGS QEDIDCRQERMS NSSEV
Sbjct: 61   PMPSLSSCNSCVSANYTSFKCDCSTVANLDVRDGQESATGSSQEDIDCRQERMSLNSSEV 120

Query: 121  AHSNDQLSGRDDGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHM 180
            A SNDQLSGRDDGSVMSN+QETTQNASGVPVNAD+EQSNSNWMDPDLWDPPEPEDPEDHM
Sbjct: 121  ADSNDQLSGRDDGSVMSNNQETTQNASGVPVNADVEQSNSNWMDPDLWDPPEPEDPEDHM 180

Query: 181  EGGMGYNDDDDDEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIR 240
            EGGMGYNDDDDDEFGD+SEWST+SSFSRSVDEASVSYRFKEEKQRA+QQVMNGK+KAFIR
Sbjct: 181  EGGMGYNDDDDDEFGDSSEWSTSSSFSRSVDEASVSYRFKEEKQRAMQQVMNGKYKAFIR 240

Query: 241  DLLKL-------GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRN 300
             LLK+       GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDP+A+VKVKCIATGTR 
Sbjct: 241  HLLKVVHVPASSGEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPNAHVKVKCIATGTRK 300

Query: 301  QSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVI 360
            QSQFVKGMVFKKHAAHKHMPTHCKNPKL+LIQGMLGEAPISRLSSFNSMDQENDFTNHVI
Sbjct: 301  QSQFVKGMVFKKHAAHKHMPTHCKNPKLVLIQGMLGEAPISRLSSFNSMDQENDFTNHVI 360

Query: 361  EMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMS 420
            EMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMS
Sbjct: 361  EMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMS 420

Query: 421  QKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVK 480
            QKTRQCDAVYFQKIVEEHAGVLEGGKM TKTLMFIEGC TRLGCTILLKGAHSDELKRVK
Sbjct: 421  QKTRQCDAVYFQKIVEEHAGVLEGGKMSTKTLMFIEGCPTRLGCTILLKGAHSDELKRVK 480

Query: 481  IVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESA 540
            +VVQCAVVMAF LILETSFIVDQ+AMFATIPFGGVP+ ASSDPQSPLEPRSP VG+AESA
Sbjct: 481  LVVQCAVVMAFQLILETSFIVDQQAMFATIPFGGVPSIASSDPQSPLEPRSPKVGQAESA 540

Query: 541  SDKSEQPTSIYISRGSHEEASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVM 600
            SD+ EQPT+I+IS GSHEEAS  SN+E  EKLI+SSEPEPYNPAIFSGFSSISDSLKR M
Sbjct: 541  SDQIEQPTNIHISSGSHEEASNESNVETVEKLIVSSEPEPYNPAIFSGFSSISDSLKRAM 600

Query: 601  GESFLSSSPYQSLSSYFGHGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQ 660
            GESFL +SPY SLSSYFGH SDLSGL+AKS SIPST QAVYQFD EVRGSSD ENSVHEQ
Sbjct: 601  GESFLLTSPYLSLSSYFGHESDLSGLVAKSDSIPSTLQAVYQFDEEVRGSSDGENSVHEQ 660

Query: 661  SVSHQSTLEGLGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIV 720
            SVSHQSTLEGLGFHETAPN + DTMQKK  LDSQSILVLMSSRNALKG MCEQSHFSHIV
Sbjct: 661  SVSHQSTLEGLGFHETAPNDTGDTMQKKPPLDSQSILVLMSSRNALKGTMCEQSHFSHIV 720

Query: 721  FYKHFDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPG 780
            FYK+FDVPLGKFLQ+NLLNQNNLCTVCG LPEAHFY+YAHYRKQLSIQVKQLPGDKVLPG
Sbjct: 721  FYKNFDVPLGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPGDKVLPG 780

Query: 781  ETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCG 840
            ETEGKLWMWSRCSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSDDTL  KSSVCG
Sbjct: 781  ETEGKLWMWSRCSKCKSLGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCG 840

Query: 841  HSLFGDFLYFFG--LGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTK 900
            HSLFGDFLYFFG  LGNMVAM RYSEVAIYTVSMPPQKLEFNSSMRQGHLI+E ENVYTK
Sbjct: 841  HSLFGDFLYFFGYVLGNMVAMFRYSEVAIYTVSMPPQKLEFNSSMRQGHLIKEIENVYTK 900

Query: 901  GVLLFTEIAHSLKKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKG 960
            G+LLF+EIAHSLKKIISER SSNVNHPQ F NDFSFVEEMLNEERSEFE+ IQNSLTKKG
Sbjct: 901  GMLLFSEIAHSLKKIISERNSSNVNHPQGFLNDFSFVEEMLNEERSEFEVKIQNSLTKKG 960

Query: 961  NPD-----FLNLNRLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSN 1020
            N D     FLNLNRLLWDLLLESCIWDRRLQSL SLGVTTNSG+SE VEPEP++LKMNSN
Sbjct: 961  NLDLAFHKFLNLNRLLWDLLLESCIWDRRLQSLASLGVTTNSGTSENVEPEPVMLKMNSN 1020

Query: 1021 IDVGPEELESTAENDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPSAIEVTEI 1080
            I+VGPEE ES AENDD+K+QQDIS++ENVLPLKEI VEG DGESGGDELNLPSAIEVTEI
Sbjct: 1021 INVGPEESESIAENDDSKIQQDISVDENVLPLKEIAVEGSDGESGGDELNLPSAIEVTEI 1080

Query: 1081 PIIDDLSPKKLSRQGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKL 1140
             IIDDLSPKKLSRQGTLSNGFD HHSDYEDSQVG+VLSSGDLH+DRTIPISIGDSTLSKL
Sbjct: 1081 QIIDDLSPKKLSRQGTLSNGFDCHHSDYEDSQVGRVLSSGDLHVDRTIPISIGDSTLSKL 1140

Query: 1141 FWTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVV 1200
            F TPFSEIRQMHLRDIQRSYFPELKSISSYTPKLL AASDFIHEEGQKLHIHL +DNFVV
Sbjct: 1141 FCTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLTAASDFIHEEGQKLHIHLADDNFVV 1200

Query: 1201 SDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDS 1260
            SDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDS
Sbjct: 1201 SDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDS 1260

Query: 1261 DSSISSEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRS 1320
            +SSISSEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRS
Sbjct: 1261 ESSISSEEYRFSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRS 1320

Query: 1321 RCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFK 1380
            RCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFK
Sbjct: 1321 RCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFK 1380

Query: 1381 YINESFDMGNQTCLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGAL 1440
            YINESFDMGNQTCLAKVLGIYQVTVRE KSGKEMRHDLMVMENLSFGRNIIRQYDLKGAL
Sbjct: 1381 YINESFDMGNQTCLAKVLGIYQVTVREQKSGKEMRHDLMVMENLSFGRNIIRQYDLKGAL 1440

Query: 1441 HARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSL 1500
            HARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKR LQRAIWNDT FLNSINVMDYSL
Sbjct: 1441 HARFNPATNNFGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTTFLNSINVMDYSL 1500

Query: 1501 LVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMS 1560
            LVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTV+SPKEYKRRFRKFMS
Sbjct: 1501 LVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMS 1560

Query: 1561 AHFLSVPDHWCMRQNLPGPWDLFDTADPASSHRTNADENDVSS 1577
            AHFLSVPDHWCM+QNLPGPWDL  TAD ASS++TNA+EN +SS
Sbjct: 1561 AHFLSVPDHWCMQQNLPGPWDLCGTADCASSNKTNANENVLSS 1603

BLAST of Chy1G009970 vs. NCBI nr
Match: XP_038899624.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Benincasa hispida])

HSP 1 Score: 2756 bits (7145), Expect = 0.0
Identity = 1410/1593 (88.51%), Postives = 1478/1593 (92.78%), Query Frame = 0

Query: 1    MCHCCGAVITDSNGKNLENGSSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60
            MCH CGAVITDS+ K LENGSS +LDDAG +CLC+VCKENQEQE MKSDN+S+SEAP LS
Sbjct: 1    MCHYCGAVITDSDSKKLENGSSLKLDDAGPLCLCKVCKENQEQETMKSDNKSTSEAPMLS 60

Query: 61   PMPSLSSCNSDCSTVANLDVRDGQESATGS-QEDIDCRQERMSRNSSEVAHSNDQLSGRD 120
             MPSLSSCNSDCSTVANLD+R GQE ATGS QEDID RQERM+ N SEV HSND LSGR 
Sbjct: 61   SMPSLSSCNSDCSTVANLDIRYGQEGATGSSQEDIDYRQERMTHNLSEVVHSNDHLSGRF 120

Query: 121  DGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDD 180
            D SVMS SQE T + SGVPVN ++EQS+SNWMD DLWDPPEPEDPEDHMEGGMGY+DDDD
Sbjct: 121  DESVMSISQEATHDGSGVPVNEEIEQSHSNWMDTDLWDPPEPEDPEDHMEGGMGYDDDDD 180

Query: 181  DEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIRDLLKL------ 240
            DEFGD++EWST+SSFSRSVDEASVSYRF+EEKQRA+QQVMNGK+KAFIR LLK       
Sbjct: 181  DEFGDSTEWSTSSSFSRSVDEASVSYRFREEKQRAMQQVMNGKYKAFIRHLLKFVGVAAT 240

Query: 241  -GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFK 300
             GEDSENWVDIVSSLSWEAATFLKPVVNGKA DP+A+VKVKCIATGTRNQSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKATDPEAHVKVKCIATGTRNQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVI 360
            KHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVI 360

Query: 361  LVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDAVYF 420
            LVEKTAARVIQEAILKKGMTLVLDMKLHRL+RIALCTGSPILTSETLMSQKTRQCDAVYF
Sbjct: 361  LVEKTAARVIQEAILKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQKTRQCDAVYF 420

Query: 421  QKIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQCAVVMAF 480
            QKIVEEHA VL+GG+ PTKTLMFIEGC TRLGCTILLKGAHSDELKRVK+VVQCAVVMAF
Sbjct: 421  QKIVEEHASVLDGGRRPTKTLMFIEGCPTRLGCTILLKGAHSDELKRVKVVVQCAVVMAF 480

Query: 481  HLILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESASDKSEQPT--- 540
            HLILETSF+VDQRAMFATIPFGGVPA  SSDP+SPLEP SPNVG+AE+ASDKSEQPT   
Sbjct: 481  HLILETSFLVDQRAMFATIPFGGVPAIVSSDPRSPLEPCSPNVGQAENASDKSEQPTDSM 540

Query: 541  SIYISRGSHEEASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSS 600
            +IYIS G+HEEAS GSN+E  EKLIMSSEPEPYNPAIFSGFSSI+DSLKRVMGESFL  S
Sbjct: 541  NIYISSGAHEEASNGSNLESVEKLIMSSEPEPYNPAIFSGFSSITDSLKRVMGESFLLPS 600

Query: 601  PYQSLSSYFGHGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSHQSTL 660
            PYQSLSSYFGHGSDLSGL+ KS SIPSTPQA  QFDVEVRGSSDEEN VHEQSVSHQSTL
Sbjct: 601  PYQSLSSYFGHGSDLSGLVPKSDSIPSTPQASDQFDVEVRGSSDEENLVHEQSVSHQSTL 660

Query: 661  EGLGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKHFDVP 720
            E LGFHET PNYS DTMQKK SLDSQSILVLMSSRNALKG MCEQSHFSHIVFYK+FDVP
Sbjct: 661  EALGFHETVPNYSEDTMQKKTSLDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDVP 720

Query: 721  LGKFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEGKLWM 780
            LGKFLQ+NLLNQNNLCTVCG LPEAHFY+YAHYRKQLSIQVKQLP DKVLPGETEGKLWM
Sbjct: 721  LGKFLQENLLNQNNLCTVCGELPEAHFYYYAHYRKQLSIQVKQLPVDKVLPGETEGKLWM 780

Query: 781  WSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLFGDFL 840
            WSRCSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSDDTL  KSSVCGHSLFGDFL
Sbjct: 781  WSRCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLPSKSSVCGHSLFGDFL 840

Query: 841  YFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFTEIAH 900
            YFFGLGNMVAM RYSEVAIYT+SMPPQKLEFNSSMRQGHLIRETENVYTKG+LLFTEIAH
Sbjct: 841  YFFGLGNMVAMFRYSEVAIYTISMPPQKLEFNSSMRQGHLIRETENVYTKGMLLFTEIAH 900

Query: 901  SLKKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKGNPDF-----L 960
            SLKKIISE KSSN+NHPQ F NDFS VEEMLNEERSEFE+NIQNSL KKG+PDF     L
Sbjct: 901  SLKKIISECKSSNLNHPQGFLNDFSLVEEMLNEERSEFEVNIQNSLGKKGSPDFAFHRYL 960

Query: 961  NLNRLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELES 1020
            NLNRLLWDLLLESCIWDRRLQSL SLGVT NSG++E VEPEP++LKMNSNIDVGPE  ES
Sbjct: 961  NLNRLLWDLLLESCIWDRRLQSLISLGVTINSGTTENVEPEPVMLKMNSNIDVGPEGSES 1020

Query: 1021 TAENDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIIDDLSPKK 1080
              ENDDTKVQ DIS++ENV P +E  VEG  GESGGDELNLP   EVTEIPI+DDLSPK+
Sbjct: 1021 IVENDDTKVQHDISVDENVSPQREAVVEGSIGESGGDELNLPLVTEVTEIPIMDDLSPKQ 1080

Query: 1081 LSRQGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQ 1140
            LSRQGTL+NGFDY HSDYE+ QVG+VLSSGDLH+DRTIPIS GDSTLSKLFWTPFSEIRQ
Sbjct: 1081 LSRQGTLTNGFDYIHSDYENPQVGRVLSSGDLHLDRTIPISTGDSTLSKLFWTPFSEIRQ 1140

Query: 1141 MHLRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSI 1200
            MH+RDIQRSYFPELKSISSYTPK LPAASDFI+EEGQKLHI L NDNF+VSDYEGELSSI
Sbjct: 1141 MHIRDIQRSYFPELKSISSYTPKFLPAASDFINEEGQKLHIRLANDNFIVSDYEGELSSI 1200

Query: 1201 VACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEYR 1260
            VACVLALLKD P Q DSHNEDSKGEGGG  QPALSSRS NKVPSNGSSDS+SSISSEEYR
Sbjct: 1201 VACVLALLKDLPLQADSHNEDSKGEGGGVSQPALSSRSQNKVPSNGSSDSESSISSEEYR 1260

Query: 1261 FSSFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFI 1320
            FSSFDRLNLLDSLV ETFKR+DHEGVIKSLAKGKYLVNCPY NQFRDLRSRCCPSELH+I
Sbjct: 1261 FSSFDRLNLLDSLVPETFKRADHEGVIKSLAKGKYLVNCPYFNQFRDLRSRCCPSELHYI 1320

Query: 1321 ASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGN 1380
            ASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGN
Sbjct: 1321 ASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGN 1380

Query: 1381 QTCLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNN 1440
            QTCLAKVLGIYQVTVRE KSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHAR+NPAT+N
Sbjct: 1381 QTCLAKVLGIYQVTVRELKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARYNPATSN 1440

Query: 1441 FGEVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKE 1500
            FGEVLLDQNFVNDMNSSPLYVSNRAKR LQRAIWNDTAFLNSINVMDYSLLVGVDAEKKE
Sbjct: 1441 FGEVLLDQNFVNDMNSSPLYVSNRAKRRLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKE 1500

Query: 1501 LVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHW 1560
            LVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTV+SPKEYKRRFRKFMSAHFLSVPDHW
Sbjct: 1501 LVCGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDHW 1560

Query: 1561 CMRQNLPGPWDLFDTADPASSHRTNADENDVSS 1577
            CM+QNLPGPWDL    D ASS +TN +END+SS
Sbjct: 1561 CMQQNLPGPWDLCGNTDCASSQKTNGEENDLSS 1593

BLAST of Chy1G009970 vs. NCBI nr
Match: XP_023535373.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita pepo subsp. pepo] >XP_023535374.1 putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2426 bits (6288), Expect = 0.0
Identity = 1262/1591 (79.32%), Postives = 1370/1591 (86.11%), Query Frame = 0

Query: 1    MCHCCGAVITDSNGKNLENGSSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60
            MCH CGAVITDS GK L NG+S  LDD+GTVC+C+VCKENQE E M  DN+SSSE   LS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICKVCKENQELETMNLDNKSSSEVLMLS 60

Query: 61   PMPSLSSCNSDCSTVANLDVRDGQESAT-GSQEDIDCRQERMSRNSSEVAHSNDQLSGRD 120
            PMPSLSSC+SDCSTVANLD++DGQE  T  SQ+ IDCRQERMS+N SEV HSN QLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDIKDGQEGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDD 180
            D SV++ SQE T N SGV VNA++EQS+SNW+D DLWDPPEPEDP+DH+EGGMGY+DDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHVEGGMGYDDDDD 180

Query: 181  D-EFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIRDLLKL----- 240
            D EF D++EW+T+SSFSRSVDEASVSYR KE+KQRA+QQ+MNGK K FI  LLK      
Sbjct: 181  DDEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVAS 240

Query: 241  -GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFK 300
             GEDSENWVDIVSSLSWEAATFLKPVVNGKA+DP+A+VKVKCIATGTR+QSQFVKGMVFK
Sbjct: 241  SGEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFK 300

Query: 301  KHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVI 360
            KHAAHKHMPTHCKNPKLILI+GMLGEAPISRLSSFNSMDQENDFT HVIEMIE CTANVI
Sbjct: 301  KHAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVI 360

Query: 361  LVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDAVYF 420
            LVEKT +RVIQEA+LKKGMTLVLDMKLHRL+RIALCTGSPILTSETLMSQ TRQCDAVYF
Sbjct: 361  LVEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYF 420

Query: 421  QKIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQCAVVMAF 480
            +KIVEEHAG+LEGGK PTKTLMFIEGC TRLGCTILLKGAHSDELK++K+VVQ AVVMAF
Sbjct: 421  EKIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAF 480

Query: 481  HLILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESASDKSEQPTSIY 540
            HL+LETSF+VDQRAMFATIPFGGVPAT SSDPQSPLEP SPN                  
Sbjct: 481  HLMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNA----------------- 540

Query: 541  ISRGSHEEASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQ 600
                  + AS GSN+E           EPYNPAIFSG SSISDSLKRVMGESF  +SP Q
Sbjct: 541  ------QGASNGSNLE----------GEPYNPAIFSGLSSISDSLKRVMGESFFLASPCQ 600

Query: 601  SLSSYFGHGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSHQSTL-EG 660
            S SSYFGHG DLSGL+AKS SIPSTP    QFDVEVRGSSDEENS+HEQ V HQST  EG
Sbjct: 601  SFSSYFGHGKDLSGLVAKSDSIPSTPPGADQFDVEVRGSSDEENSIHEQFVPHQSTFDEG 660

Query: 661  LGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLG 720
             GFHETAPNYS D +QKK   DSQSILVLMSSRNALKG MCEQSHFSHIVFYK+FD PLG
Sbjct: 661  FGFHETAPNYSEDKLQKKTPFDSQSILVLMSSRNALKGTMCEQSHFSHIVFYKNFDAPLG 720

Query: 721  KFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWS 780
            KFLQ+NLLNQ N+CTVCG LPEAH+Y+YAH RKQL IQVKQLP +KVLPGETEGKLWMWS
Sbjct: 721  KFLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVNKVLPGETEGKLWMWS 780

Query: 781  RCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYF 840
            RCSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSD TL  KSSVCGHSLFGDFLYF
Sbjct: 781  RCSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSLFGDFLYF 840

Query: 841  FGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSL 900
            FGLGNMVAM RY+EVAIYTVSMPPQKLEFNSSMRQGHLI ETENVY KG++LFTEIA  L
Sbjct: 841  FGLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITETENVYAKGMILFTEIAQFL 900

Query: 901  KKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKGNPDF-----LNL 960
            KKIISERKSS VN       DFS +EEMLNEERSEFE NIQ+SLTKKGNPDF     L+L
Sbjct: 901  KKIISERKSSTVN-------DFSLLEEMLNEERSEFEQNIQSSLTKKGNPDFPFHKYLSL 960

Query: 961  NRLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELESTA 1020
            NRLLW+LLLESCIWD+RLQ L S+GVT NSG+SE VEPEP          VG E LES A
Sbjct: 961  NRLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP----------VGHEGLESIA 1020

Query: 1021 ENDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIIDDLSPKKLS 1080
            + DD  VQQD+S++EN L  KEI VE LDGES GDEL LPSA EVTEIPI+DDLSPK+LS
Sbjct: 1021 QTDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPIMDDLSPKQLS 1080

Query: 1081 RQGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMH 1140
            RQG+LSNGF+  HSD ED Q G+VLSSGD      IP S G+ TL KLFWTPFSEI+QM 
Sbjct: 1081 RQGSLSNGFNRRHSDDEDPQAGRVLSSGD------IPSSTGNLTLDKLFWTPFSEIQQMR 1140

Query: 1141 LRDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSIVA 1200
            +RDIQRSYFPELKSIS+YTPKLLP A DFI++EGQKLHI L N+ F+VSDYEGELSSIVA
Sbjct: 1141 MRDIQRSYFPELKSISNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELSSIVA 1200

Query: 1201 CVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFS 1260
            CVLALLKD   +TD HNEDSKGEG            LNKVPSNGSSDSDSS+SSEE++FS
Sbjct: 1201 CVLALLKDLSLETDLHNEDSKGEG------------LNKVPSNGSSDSDSSVSSEEHQFS 1260

Query: 1261 SFDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIAS 1320
            SFDRLNLLDSL+ +TFKR++HEG+IKSLAKGKYLVNCPY NQFRDLR RCCPSELH+IAS
Sbjct: 1261 SFDRLNLLDSLLPKTFKRAEHEGIIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIAS 1320

Query: 1321 LSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQT 1380
            LSRCI+WNAKGGKSKSFFAKTLDDRFIIKEIKRTEY+SFMKFAP+YFKYINESFDMGNQ+
Sbjct: 1321 LSRCIHWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQS 1380

Query: 1381 CLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFG 1440
            CLAKVLGIYQVTVR+PKSGKE+RH+LMVMENLSFGRNIIRQYDLKGALHAR+NPATN+FG
Sbjct: 1381 CLAKVLGIYQVTVRDPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFG 1440

Query: 1441 EVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELV 1500
            EVLLDQNFVNDMNSSPLYV+N+AKR LQRAIWNDTAFLNS+NVMDYSLLVGVDAEKKELV
Sbjct: 1441 EVLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELV 1500

Query: 1501 CGIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWCM 1560
            CGIIDYLRQYTWDKQLETW KSSL PKNVLPTV+SPKEYKRRFRKFMSAHFLSVPD+WC 
Sbjct: 1501 CGIIDYLRQYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQ 1522

Query: 1561 RQNLPGPWDLFDTADPASSHRTNADENDVSS 1577
            +QNL GPW      D ASS +TNA+END+SS
Sbjct: 1561 QQNLHGPWGACGITDRASS-QTNAEENDLSS 1522

BLAST of Chy1G009970 vs. NCBI nr
Match: XP_022932526.1 (putative 1-phosphatidylinositol-3-phosphate 5-kinase FAB1D [Cucurbita moschata])

HSP 1 Score: 2424 bits (6282), Expect = 0.0
Identity = 1259/1590 (79.18%), Postives = 1367/1590 (85.97%), Query Frame = 0

Query: 1    MCHCCGAVITDSNGKNLENGSSHQLDDAGTVCLCRVCKENQEQEMMKSDNRSSSEAPTLS 60
            MCH CGAVITDS GK L NG+S  LDD+GTVC+CRVCKENQE + M  DN+SSSEA  LS
Sbjct: 1    MCHYCGAVITDSEGKRLGNGNSFDLDDSGTVCICRVCKENQELKTMNLDNKSSSEALMLS 60

Query: 61   PMPSLSSCNSDCSTVANLDVRDGQESAT-GSQEDIDCRQERMSRNSSEVAHSNDQLSGRD 120
            PMPSLSSC+SDCSTVANLDV+DGQ+  T  SQ+ IDCRQERMS+N SEV HSN QLSGRD
Sbjct: 61   PMPSLSSCDSDCSTVANLDVKDGQDGTTRSSQDSIDCRQERMSQNWSEVVHSNGQLSGRD 120

Query: 121  DGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDD 180
            D SV++ SQE T N SGV VNA++EQS+SNW+D DLWDPPEPEDP+DHMEGGMGY+DDDD
Sbjct: 121  DESVITISQEATHNGSGVRVNAEIEQSHSNWLDTDLWDPPEPEDPQDHMEGGMGYDDDDD 180

Query: 181  DEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIRDLLKL------ 240
            DEF D++EW+T+SSFSRSVDEASVSYR KE+KQRA+QQ+MNGK K FI  LLK       
Sbjct: 181  DEFDDSTEWNTSSSFSRSVDEASVSYRIKEQKQRAMQQIMNGKFKVFIGHLLKFVGVASS 240

Query: 241  GEDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFKK 300
            GEDSENWVDIVSSLSWEAATFLKPVVNGKA+DP+A+VKVKCIATGTR+QSQFVKGMVFKK
Sbjct: 241  GEDSENWVDIVSSLSWEAATFLKPVVNGKAVDPEAHVKVKCIATGTRSQSQFVKGMVFKK 300

Query: 301  HAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQENDFTNHVIEMIEGCTANVIL 360
            HAAHKHMPTHCKNPKLILI+GMLGEAPISRLSSFNSMDQENDFT HVIEMIE CTANVIL
Sbjct: 301  HAAHKHMPTHCKNPKLILIEGMLGEAPISRLSSFNSMDQENDFTKHVIEMIEVCTANVIL 360

Query: 361  VEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQKTRQCDAVYFQ 420
            VEKT +RVIQEA+LKKGMTLVLDMKLHRL+RIALCTGSPILTSETLMSQ TRQCDAVYF+
Sbjct: 361  VEKTTSRVIQEAMLKKGMTLVLDMKLHRLERIALCTGSPILTSETLMSQNTRQCDAVYFE 420

Query: 421  KIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQCAVVMAFH 480
            KIVEEHAG+LEGGK PTKTLMFIEGC TRLGCTILLKGAHSDELK++K+VVQ AVVMAFH
Sbjct: 421  KIVEEHAGLLEGGKRPTKTLMFIEGCPTRLGCTILLKGAHSDELKKIKVVVQIAVVMAFH 480

Query: 481  LILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESASDKSEQPTSIYI 540
            L+LETSF+VDQRAMFATIPFGGVPAT SSDPQSPLEP SPN                   
Sbjct: 481  LMLETSFLVDQRAMFATIPFGGVPATVSSDPQSPLEPCSPNA------------------ 540

Query: 541  SRGSHEEASTGSNMELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQS 600
                 + AS GSN+E           EPYNPAIFSG SSISDSLKRVMGESF  +SP QS
Sbjct: 541  -----QGASNGSNLE----------GEPYNPAIFSGLSSISDSLKRVMGESFFLASPCQS 600

Query: 601  LSSYFGHGSDLSGLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSHQSTL-EGL 660
             SSYFGHG DLSGL+AKS SIPSTP    QFDVEVRGSSDEENS+HEQ V HQST  EG 
Sbjct: 601  FSSYFGHGKDLSGLVAKSDSIPSTPPEADQFDVEVRGSSDEENSIHEQFVPHQSTFDEGF 660

Query: 661  GFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLGK 720
            GFHETAPNYS D + +K   +SQSILVLMSSRNA KG MCEQSHFSHIVFYK+FD PLGK
Sbjct: 661  GFHETAPNYSEDKLPEKTPFNSQSILVLMSSRNASKGTMCEQSHFSHIVFYKNFDAPLGK 720

Query: 721  FLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSR 780
            FLQ+NLLNQ N+CTVCG LPEAH+Y+YAH RKQL IQVKQLP +KVLPGETEGKLWMWSR
Sbjct: 721  FLQENLLNQKNVCTVCGELPEAHYYYYAHSRKQLCIQVKQLPVNKVLPGETEGKLWMWSR 780

Query: 781  CSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYFF 840
            CSKCKS GGPSKSTKRVLISTAARGLSFGKFLELCFSD TL  KSSVCGHSLFGDFLYFF
Sbjct: 781  CSKCKSKGGPSKSTKRVLISTAARGLSFGKFLELCFSDCTLPSKSSVCGHSLFGDFLYFF 840

Query: 841  GLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSLK 900
            GLGNMVAM RY+EVAIYTVSMPPQKLEFNSSMRQGHLI ETENVY KG++LFTEIA  LK
Sbjct: 841  GLGNMVAMFRYTEVAIYTVSMPPQKLEFNSSMRQGHLITETENVYAKGMILFTEIAQFLK 900

Query: 901  KIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSLTKKGNPDF-----LNLN 960
            KIISERKSS VN       DFS +EEMLNEERSEFELNIQ+SLTKKGNPDF     L+LN
Sbjct: 901  KIISERKSSTVN-------DFSLLEEMLNEERSEFELNIQSSLTKKGNPDFPFHKYLSLN 960

Query: 961  RLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELESTAE 1020
            RLLW+LLLESCIWD+RLQ L S+GVT NSG+SE VEPEP          VG E LES A+
Sbjct: 961  RLLWELLLESCIWDKRLQLLTSVGVTLNSGTSENVEPEP----------VGQEGLESIAQ 1020

Query: 1021 NDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIIDDLSPKKLSR 1080
             DD  VQQD+S++EN L  KEI VE LDGES GDEL LPSA EVTEIPI+DDLSPK+LSR
Sbjct: 1021 TDDRNVQQDVSVDENALHPKEIAVEHLDGESDGDELGLPSATEVTEIPIMDDLSPKQLSR 1080

Query: 1081 QGTLSNGFDYHHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMHL 1140
            QG+LSNGF+  HSD ED Q  +VLSSGD      IP S G+ TL KLFWTPFSEI+QM +
Sbjct: 1081 QGSLSNGFNRRHSDDEDPQAVRVLSSGD------IPSSTGNLTLDKLFWTPFSEIQQMRM 1140

Query: 1141 RDIQRSYFPELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSIVAC 1200
            RDIQRSYFPELKS+S+YTPKLLP A DFI++EGQKLHI L N+ F+VSDYEGELSSIVAC
Sbjct: 1141 RDIQRSYFPELKSVSNYTPKLLPTAHDFINKEGQKLHIFLSNEKFIVSDYEGELSSIVAC 1200

Query: 1201 VLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFSS 1260
            VLALLKD   +TD HNEDSKGEG            LNKVPSNGSSDSDSS+SSEE++FSS
Sbjct: 1201 VLALLKDLSLETDLHNEDSKGEG------------LNKVPSNGSSDSDSSVSSEEHQFSS 1260

Query: 1261 FDRLNLLDSLVSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASL 1320
            FDRLNLLDSL+ +TFKR++HEGVIKSLAKGKYLVNCPY NQFRDLR RCCPSELH+IASL
Sbjct: 1261 FDRLNLLDSLLPKTFKRAEHEGVIKSLAKGKYLVNCPYFNQFRDLRRRCCPSELHYIASL 1320

Query: 1321 SRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTC 1380
            SRCI+WNAKGGKSKSFFAKTLDDRFIIKEIKRTEY+SFMKFAP+YFKYINESFDMGNQTC
Sbjct: 1321 SRCIHWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYESFMKFAPDYFKYINESFDMGNQTC 1380

Query: 1381 LAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGE 1440
            LAKVLGIYQVTVREPKSGKE+RH+LMVMENLSFGRNIIRQYDLKGALHAR+NPATN+FGE
Sbjct: 1381 LAKVLGIYQVTVREPKSGKEVRHELMVMENLSFGRNIIRQYDLKGALHARYNPATNDFGE 1440

Query: 1441 VLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVC 1500
            VLLDQNFVNDMNSSPLYV+N+AKR LQRAIWNDTAFLNS+NVMDYSLLVGVDAEKKELVC
Sbjct: 1441 VLLDQNFVNDMNSSPLYVNNQAKRRLQRAIWNDTAFLNSMNVMDYSLLVGVDAEKKELVC 1500

Query: 1501 GIIDYLRQYTWDKQLETWVKSSLIPKNVLPTVISPKEYKRRFRKFMSAHFLSVPDHWCMR 1560
            GIIDYLRQYTWDKQLETW KSSL PKNVLPTV+SPKEYKRRFRKFMSAHFLSVPD+WC +
Sbjct: 1501 GIIDYLRQYTWDKQLETWAKSSLRPKNVLPTVVSPKEYKRRFRKFMSAHFLSVPDNWCQQ 1522

Query: 1561 QNLPGPWDLFDTADPASSHRTNADENDVSS 1577
            QNL GPW         +S +TNA+END+SS
Sbjct: 1561 QNLHGPWGACGGTIDRASSQTNAEENDLSS 1522

BLAST of Chy1G009970 vs. TAIR 10
Match: AT1G34260.1 (FORMS APLOID AND BINUCLEATE CELLS 1A )

HSP 1 Score: 1111.7 bits (2874), Expect = 0.0e+00
Identity = 704/1537 (45.80%), Postives = 928/1537 (60.38%), Query Frame = 0

Query: 57   PTLSPMPSLSSCNSDCSTVANLDVR--DGQESATGSQEDIDCRQE----RMSRNSSEVAH 116
            P+ S   S  S + +CS   N   R  + + S+  SQED++  +E    R+ R S  +  
Sbjct: 3    PSNSLSSSERSLSGECSVDGNSCDRGIEDECSSHSSQEDVELTKEVKVDRLERKSKSM-- 62

Query: 117  SNDQLSGRDDGSVMSNSQETTQNASGVPVNADLEQSNSNWMDPDLWDPPEPEDPEDHMEG 176
             +D L   D+ S   NS E  Q  S    ++D         D  +W+PPEPE+PED ++G
Sbjct: 63   PSDILDILDEKS-KENSVENVQFLSDREDDSD---------DVPVWEPPEPENPEDEVDG 122

Query: 177  GMGYNDDDDDEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRALQQVMNGKHKAFIRDL 236
                  DDDD+  D S+W+ AS      DE+S   +  EE +R + +  + K K  +  L
Sbjct: 123  VFA---DDDDDCCDGSKWNKASLLGELSDESSEKRKVYEENRRVMLEEADSKFKFIVSQL 182

Query: 237  LKLG----EDSENWVDIVSSLSWEAATFLKPVVNGKAMDPDANVKVKCIATGTRNQSQFV 296
            +K      E+S  W +IV+ L WEAA+ LKP ++GK++DP   +KVKCIATG+   S+  
Sbjct: 183  IKSAGFSIEESGYWFEIVARLCWEAASMLKPAIDGKSVDPTEYIKVKCIATGSCVDSEVF 242

Query: 297  KGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPISRLSSFNSMDQEND----FTNHVIE 356
            KG+VFKKHAA KHM T  ++P+++L++G+LG  PIS  SS  S++Q+N+    +   V++
Sbjct: 243  KGLVFKKHAALKHMATKYEHPRIMLVEGVLGH-PISGFSSLQSVNQDNEYLLKYVKPVVD 302

Query: 357  MIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQRIALCTGSPILTSETLMSQ 416
            +IE    +V+LVEK+ +R IQ+ IL KG+TLV DMKLHRLQRI+ C GSPIL+ ++L SQ
Sbjct: 303  IIEASKPDVMLVEKSVSRDIQKTILDKGVTLVFDMKLHRLQRISRCIGSPILSVDSLSSQ 362

Query: 417  KTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCSTRLGCTILLKGAHSDELKRVKI 476
            K + CD+   +KIVEEH    E  K PTKTLMF+EGC TRLGCTILLKG HS+ LK+VK 
Sbjct: 363  KLKHCDSFRIEKIVEEHNAAGESDKKPTKTLMFLEGCPTRLGCTILLKGCHSERLKKVKE 422

Query: 477  VVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSDPQSPLEPRSPNVGKAESAS 536
            VVQ + ++A+HL+LE SF+ D+  MF+TI      A  ++     +E  SP+    ES S
Sbjct: 423  VVQYSFILAYHLMLEASFLADRHTMFSTI-----FAKEATSCVVEIENFSPSPSPRESPS 482

Query: 537  DKSEQPTSIYISRGSHEEASTGSNMELAEKLIMSSEP--------EPYNPAIFSGFSSIS 596
            +  + P S        +E +   N E   + + + E         EPYNP IF+GFSS+S
Sbjct: 483  EAVDIPVS-----NGFDEQTIQINGEADGEKVGTWESDGDHVFSHEPYNPVIFTGFSSLS 542

Query: 597  DSLKRVMGESFLSSSPYQSLSSYFGHGSDLSGLIAKSSSIPSTPQ----AVYQFDVEVRG 656
              L + +G      S   S+ +     S+L  +           +     +   ++ V  
Sbjct: 543  ARLSKYLGFVQNPESVPVSVDTDVSTTSNLDSIRESEEDTAEKNEDKQPLLLDPELPVNS 602

Query: 657  SSDEENSVHEQSVSHQSTLEGLGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGA 716
            SSD+ ++  +     +STLE                       SQSILVL+S RNAL+G 
Sbjct: 603  SSDDGDNKSQTENDIESTLE-----------------------SQSILVLVSKRNALRGI 662

Query: 717  MCEQSHFSHIVFYKHFDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQV 776
            MC+Q HFSHI FYKHFDVPL KFL+D + NQ NLC  C   PEAH Y+YAH  KQL+IQ+
Sbjct: 663  MCDQRHFSHIKFYKHFDVPLEKFLRD-MFNQRNLCQTCVEFPEAHLYYYAHQNKQLTIQI 722

Query: 777  KQLPGDKVLPGETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSD 836
            K++P  K L GE +GK+WMWSRC KCK+     KSTKRVLISTAAR LSFGKFLEL FS 
Sbjct: 723  KRIPVAKGLAGEAKGKIWMWSRCGKCKTKNASRKSTKRVLISTAARSLSFGKFLELSFSQ 782

Query: 837  DTLLGKSSVCGHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLI 896
             T L +SS CGHS   DFL+FFGLG+MVAM  YS+VA YTVS+PP KLE +  ++ G L 
Sbjct: 783  QTFLNRSSSCGHSFDSDFLHFFGLGSMVAMLSYSQVASYTVSLPPMKLESSILIKAGWLE 842

Query: 897  RETENVYTKGVLLFTEIAHSLKKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELN 956
            +E + V+TKG+ LF + A  LK++ S+  +S++ + Q      S +EE+L  ER  FE N
Sbjct: 843  KEFQTVFTKGISLFEDAAGFLKRLRSQFTNSDLRY-QRARKLLSNIEELLKHERCIFEEN 902

Query: 957  IQNSLTKKGNPD-----FLNLNRLLWDLLLESCIWDRRLQSL-RSLGVTTNSGSSEIVEP 1016
            I+NS  K    D      L LNR+ W+LLL++ IW+ RLQSL  S  +  +S  ++I E 
Sbjct: 903  IKNSFDKAKTIDDVSHRLLRLNRMRWELLLQALIWNYRLQSLVLSDRLLPSSDETKIYEQ 962

Query: 1017 EPLLLKMNSNIDVGPEELESTAENDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELN 1076
                LK  S       E   T   +D KV    S      PL E     + G S GD   
Sbjct: 963  G---LKTVS-------EAGMTRYENDNKVSDSGSNGGIDTPLVEHKDIPIAGASVGDNDQ 1022

Query: 1077 LPSAI--EVTEIPIIDDLSPKKLSRQGTLSNGFDYH-----HSDYEDSQVGKVLSSGDLH 1136
            +  +   E  E   +   SP   S    ++N FD H     HS         +  +G+  
Sbjct: 1023 MAESYVPEDNESQTLCSSSPDTTS---PINNHFDTHLAVNVHSTNGQEADKSIPVTGE-S 1082

Query: 1137 IDRTIPISIGDSTL--SKLFWTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLPAASDF 1196
            +D  +  S G   L   + FW PF E+R   + DI++ Y  + + ++++T + L   +  
Sbjct: 1083 LDDEVSTSNGPHILGWDEWFWLPFEELRSKRIVDIEKEYLLKFEYVNNFTQENLQTVNQI 1142

Query: 1197 IHEEGQKLHIHLDNDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEGGGALQ 1256
            I EE  +L I L +D+F+VSDYE ELSS++AC LA L         +NE+SK      + 
Sbjct: 1143 ITEESSRLRISLRDDDFIVSDYEDELSSLIACALAHL---------NNEESKKPLSRCIH 1202

Query: 1257 PALSSRSLNKVPSNGSSDSDSSISSEEYRFSSFDRLNLLDSL----VSETFKRSDHEGVI 1316
             +L     N   +  S  +D  +S    RFSS +  N L++L    V  TF      G +
Sbjct: 1203 GSLQGFLDN---NQDSKQTDRDVS----RFSS-ESTNRLETLPPPEVLVTF------GSV 1262

Query: 1317 KSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAKTLDDR 1376
            KS+ K KY +   Y + FRDLR RCC SEL +IASLSRC  W+AKGGKSKS FAKTLDDR
Sbjct: 1263 KSVGKPKYSIVSLYADDFRDLRKRCCSSELDYIASLSRCKPWDAKGGKSKSVFAKTLDDR 1322

Query: 1377 FIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREPK-SGKEMRH 1436
            FI+KEIK+TEY+SF+ FA EYFKY+ +S+D+GNQTCLAKVLGI+QVTVR+PK  GKE+RH
Sbjct: 1323 FIVKEIKKTEYESFVTFATEYFKYMKDSYDLGNQTCLAKVLGIHQVTVRQPKGGGKEIRH 1382

Query: 1437 DLMVMENLSFGRNIIRQYDLKGALHARFN-PATNNFGEVLLDQNFVNDMNSSPLYVSNRA 1496
            DLMVMENLSF R + RQYDLKGALHARF   + N   +VLLDQNFVNDMN SPLYVS  +
Sbjct: 1383 DLMVMENLSFSRKVTRQYDLKGALHARFTATSANGEDDVLLDQNFVNDMNKSPLYVSKTS 1442

Query: 1497 KRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWVKSS 1546
            K+ LQRA++NDT+FL SINVMDYSLLVGVD E  ELVCGIIDYLRQYTWDKQLETWVKSS
Sbjct: 1443 KQNLQRAVYNDTSFLTSINVMDYSLLVGVDDENHELVCGIIDYLRQYTWDKQLETWVKSS 1451

BLAST of Chy1G009970 vs. TAIR 10
Match: AT1G71010.1 (FORMS APLOID AND BINUCLEATE CELLS 1C )

HSP 1 Score: 754.2 bits (1946), Expect = 2.1e-217
Identity = 525/1441 (36.43%), Postives = 783/1441 (54.34%), Query Frame = 0

Query: 155  LWDPPEPEDPEDHMEGGMGYNDDDDDEFGDN-SEWSTASSFSRSVDEASVSYRFKEEKQR 214
            +W PP PED  D  E    + DD+DD+ GD+ +E+S +SSFS  +       +  E    
Sbjct: 263  IWYPPPPEDENDDAESNYFHYDDEDDDIGDSATEFSLSSSFSSHI---PTKEKLGENSNE 322

Query: 215  ALQQVMNGKHKAFIRDLLKLGED--------SENWVDIVSSLSWEAATFLKPVVN-GKAM 274
             L+ V++   +A + +LL+ GE+        +  W+DIV++L+W+AA F+KP    G +M
Sbjct: 323  PLRTVVHDHFRALVAELLR-GEELSPSDDGSAGEWLDIVTALAWQAANFVKPDTRAGGSM 382

Query: 275  DPDANVKVKCIATGTRNQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPIS-R 334
            DP   VK+KC+A+G +N+S  ++G+V  K+  HK M +  KNP+++L+ G L    ++ +
Sbjct: 383  DPGNYVKIKCVASGNQNESILIRGIVCSKNITHKRMISQYKNPRVMLLAGSLEYQRVAGQ 442

Query: 335  LSSFNS-MDQENDFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRL 394
            L+SFN+ + QEN+    +I  IE    NV+LVEK+A+   Q+ +L+K ++LVL++K   L
Sbjct: 443  LASFNTLLQQENEHMKAIIAKIESLRPNVLLVEKSASSYAQQYLLEKEISLVLNVKRSLL 502

Query: 395  QRIALCTGSPILTS-ETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCST 454
             RIA CTG+ +  S +++ + +   C+    ++++E+H    +  + P++TLM+ EGC  
Sbjct: 503  DRIARCTGAVLCPSLDSISTARLGHCELFRTERVLEQHEAGNQSNRKPSRTLMYFEGCPR 562

Query: 455  RLGCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETSFIVDQRAMFATIPF--GGVPAT 514
            RLGCT++L+G+  +ELK+VK V+Q AV  A+HL LETSF+ D+ A    I     G+  T
Sbjct: 563  RLGCTVVLRGSCREELKKVKHVIQYAVFAAYHLSLETSFLADEGASLPKIRLKQPGMVRT 622

Query: 515  ASSDPQSPLEPRSPNVGKAESASDKSEQPTSIYISRGSHEEASTGSNMELAEKLIMSSEP 574
            AS       + R  + G +      +E  +   +   +HE+  T     + E  +  S  
Sbjct: 623  AS-------QRRIIDEGISLITQSPTETDSQALLETAAHEDEHTA---PMPEHEVCESLC 682

Query: 575  EPYNPA-IFSGFSSISDSLKRVMGESFLSSSPYQSLSSYFGHGSDL-SGLIAKSSSIPST 634
            E ++P  IF   S +       +   F ++   +S SS     +DL    +  SS IP T
Sbjct: 683  EDFDPTQIFPPSSEVETEQSDTLNGDFANNLVTRSYSS--NQLNDLHEPTLCLSSEIPET 742

Query: 635  PQAVYQFDVEVRGSSDEENSVHEQSVSHQSTLEGLGFHETAPNYSVDTMQKKMSLDSQSI 694
            P      + E  G  +EEN    Q V+ Q     L  HE+     V +     +   QSI
Sbjct: 743  PTQQPSGE-EDNGRGEEEN----QLVNPQD----LPQHESFYEDDVSSEYFSAADSHQSI 802

Query: 695  LVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFY 754
            LV  SSR  LK ++CE+S    I FY  FD PLG++L+D+L ++ + C  C  L +AH  
Sbjct: 803  LVSFSSRCVLKESVCERSRLLRIKFYGSFDKPLGRYLKDDLFDKTSSCRSCKELVDAHVL 862

Query: 755  FYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARG 814
             Y+H    L+I V++LP  K LPGE +GK+WMW RC +C    G   +T+RV++S AA G
Sbjct: 863  CYSHQNGNLTINVRRLPSMK-LPGEQDGKIWMWHRCLRCAHVDGVPPATRRVVMSDAAWG 922

Query: 815  LSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQK 874
            LSFGKFLEL FS+     + + CGHSL  D L F+G GNMVA  RYS + I TV +PP  
Sbjct: 923  LSFGKFLELSFSNHATANRVASCGHSLQRDCLRFYGFGNMVAFFRYSPINILTVLLPPSM 982

Query: 875  LEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSLKKIISERKSSNVNHPQCFPNDFSF-- 934
            LEFNS  +Q  +  E   +  K   ++TEI+  L ++  E KSS +   Q    D     
Sbjct: 983  LEFNSHPQQEWIRTEAAELVGKMRTMYTEISDMLNRM--EEKSSLLEPEQSEACDLHSRI 1042

Query: 935  --VEEMLNEERSEFELNIQ----NSLTKKGNPDFLNLNRLLWDLLLESCIWDRRLQSLRS 994
              + + L +E+ E++  +Q     +L  +G+ D L LNRL   L++ +  WD +L  L S
Sbjct: 1043 IGLIDQLVKEKDEYDDALQPIFEENLQIQGSLDILELNRLRRALMIGAHAWDHQLYLLNS 1102

Query: 995  ---------LGVTTNSGSSEIVEPEPLLLKMNSNIDVGPEELESTAE-NDDTKVQQDISL 1054
                      G      + E+ +P  +  +M    D   E+  + +E N D K  ++I  
Sbjct: 1103 QLKKASVFKTGDDNAPRNPEMHDPPKIDRRMQEGSDERDEQSHTDSEANGDNKDPENIPS 1162

Query: 1055 EENVLPLKEITVEGLDGESGGDELNLPSAIEVTEIPIID--DLSPKKLSRQGTLSNGFDY 1114
                L       E +D    G   NL  A  + E       + S ++L+R   + + FD 
Sbjct: 1163 PGTSLS------ERIDSAWLGSFQNLEKAETIAETEGFSAVNSSLRRLARPIRVQS-FDS 1222

Query: 1115 HHSDYEDSQVGKVLSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMHLRDIQRSYFPE 1174
                 E  Q G   SS  L   R+   S        +   P S + + + + +      +
Sbjct: 1223 AIRFQERIQKGLPPSSLYLSTLRSFHAS---GEYRNMVRDPVSNVMRTYSQMLPLE-VQK 1282

Query: 1175 LKSISSYTPKLLPAASDFIHEEGQKLHI-HLDNDNFVVSDYEGELSSIVACVLALLKDQP 1234
            L  I    P  + +AS     +G ++ I     ++ VV  Y+ + +S+V+  +       
Sbjct: 1283 LDLIVGSAPTYISSASQM--ADGARMLIPQRGLNDIVVPVYDDDPASVVSYAI------- 1342

Query: 1235 FQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSD--------SSISSEEYRFSSF 1294
              +  + E    +G   L  + SS +LN   S  S+ S           I    Y  S  
Sbjct: 1343 -NSKEYKEWIVNKG---LASSSSSSNLNNRESEPSAFSTWRSLSMDVDYIQHAVYGSSQD 1402

Query: 1295 DRLNLLDSLVSETFKRSDHEGVIKSLAKG--KYLVNCPYINQFRDLRSRCCPSELHFIAS 1354
            DR +        T   SD      +  +G  K+ V C +  QF  LR  CCPSE+ F+ S
Sbjct: 1403 DRKS-----PHLTISFSDRASSSSTATEGKVKFSVTCYFATQFDTLRKTCCPSEVDFVRS 1462

Query: 1355 LSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQT 1414
            LSRC  W+A+GGKS  +FAK+LD+RFIIK++ +TE DSF  FAPEYFKY+ ES   G+ T
Sbjct: 1463 LSRCQRWSAQGGKSNVYFAKSLDERFIIKQVVKTELDSFEDFAPEYFKYLKESLSSGSPT 1522

Query: 1415 CLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFG 1474
            CLAK+LGIYQV+++ PK GKE + DLMVMENL + R I R YDLKG+  +R+NP T+   
Sbjct: 1523 CLAKILGIYQVSIKHPKGGKETKMDLMVMENLFYNRRISRIYDLKGSARSRYNPNTSGAD 1582

Query: 1475 EVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELV 1534
            +VLLD N +  + + P+++ ++AKR L+RAIWNDT FL S++VMDYSLLVG D E+KELV
Sbjct: 1583 KVLLDMNLLETLRTEPIFLGSKAKRSLERAIWNDTNFLASVDVMDYSLLVGFDEERKELV 1642

Query: 1535 CGIIDYLRQYTWDKQLETWVKSSLI---PKNVLPTVISPKEYKRRFRKFMSAHFLSVPDH 1545
             GIID++RQYTWDK LETWVK+S I   PKN  PT++SPK+YKRRFRK M+ +FL+VP+ 
Sbjct: 1643 LGIIDFMRQYTWDKHLETWVKASGILGGPKNASPTIVSPKQYKRRFRKAMTTYFLTVPEP 1646

BLAST of Chy1G009970 vs. TAIR 10
Match: AT3G14270.1 (phosphatidylinositol-4-phosphate 5-kinase family protein )

HSP 1 Score: 747.3 bits (1928), Expect = 2.6e-215
Identity = 556/1566 (35.50%), Postives = 805/1566 (51.40%), Query Frame = 0

Query: 84   QESATGSQEDIDCRQERMSRNSSEVAHSNDQLSGRDDGSVMSNSQETTQNASGVPVNADL 143
            Q+S +GS     C  E + R  SE      +  GRD+    S+  + + +    PV  D 
Sbjct: 257  QKSLSGSPLIHQC-LESLIREGSEQFQKKSEHDGRDECEA-SSPADISDDQVVEPV--DF 316

Query: 144  EQSNSNWMDPDLWDPPEPEDPEDHMEGGMGYNDDDDDEFGDNSEWS-TASSFSRSVDEAS 203
            E +        LW PPEPE+ ED  E  +   D++D+E   + EW     S S    E  
Sbjct: 317  ENNGL------LWVPPEPENEEDERESAL--FDEEDNEGDASGEWGYLRPSTSFGSGEYR 376

Query: 204  VSYRFKEEKQRALQQVMNGKHKAFIRDLLKL-------GEDSENWVDIVSSLSWEAATFL 263
               R  EE ++A++ V++G  +A +  LL++        E  E+W++I++SLSWEAA  L
Sbjct: 377  GEDRTTEEHKKAMKNVVDGHFRALLAQLLQVENISVSDEEGKESWLEIITSLSWEAANLL 436

Query: 264  KP-VVNGKAMDPDANVKVKCIATGTRNQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQG 323
            KP +     MDP   VKVKC+A+G R+ S  VKG+V KK+  ++ M T  +  +L+++ G
Sbjct: 437  KPDMSKSGGMDPGGYVKVKCLASGFRHDSMVVKGVVCKKNVVNRRMSTKIEKARLLILGG 496

Query: 324  MLGEAPIS-RLSSFNS-MDQENDFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMT 383
             L    +S +LSSF++ + QE D     +  I     N++LVEK+ +R  QE +L K ++
Sbjct: 497  GLEYQRVSNQLSSFDTLLQQEKDHLKMAVAKIHAERPNILLVEKSVSRFAQEYLLAKDIS 556

Query: 384  LVLDMKLHRLQRIALCTGSPILTS-ETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMPTK 443
            LVL++K   L RIA CTG+ I+ S + L SQK   C+     +  EEH    + GK   K
Sbjct: 557  LVLNIKRPLLDRIARCTGAQIIPSVDHLSSQKLGYCENFRVDRYPEEHGSTGQVGKKVVK 616

Query: 444  TLMFIEGCSTRLGCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETSFIVDQRAMFATI 503
            TLM+ E C   LG TILL+GA+ DELK+VK VVQ  V  A+HL LETSF+ D+ A    +
Sbjct: 617  TLMYFEHCPKPLGFTILLRGANEDELKKVKHVVQYGVFAAYHLALETSFLADEGAS-PEL 676

Query: 504  PFGGVPATASSDPQSPLEPRSPNVGKAESASDKSEQPTSIYISRGSH-------EEASTG 563
            P       A  D  + +E RS +     + S   + PT +  +           E  ST 
Sbjct: 677  PLNSPITVALPDKSTSIE-RSISTVPGFTVSTYEKSPTMLSCAEPQRANSVPVSELLSTT 736

Query: 564  SNMELAEKLIMSSEPEPY---------NPAIFSGFSSISDSLKRVMGESFLS-------- 623
            +N+ + + +     P PY         NP+      +IS +L   + ES  S        
Sbjct: 737  TNLSIQKDI----PPIPYGSGWQAREINPSFVFSRHNISLNLPDRVIESRNSDLSGRSVP 796

Query: 624  -------SSPYQSLSSYFGHGSDLS--GLIAKSSSIPSTPQAVYQFDVEVRGSSDEENSV 683
                   S+P         +   LS  G + KSS I ++     Q +      + ++N+ 
Sbjct: 797  VDTPADKSNPIVVADETTNNSLHLSGQGFVRKSSQIGTSIMVENQDNGSELTIAQQQNNE 856

Query: 684  HEQSVSHQSTLEGLGFHETAPNYSVDTMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFS 743
              +    Q         E  P+ S          D QSILV +SSR+  KG +CE+SH  
Sbjct: 857  KPKETQSQK-------EEFPPSPS----------DHQSILVSLSSRSVWKGTVCERSHLF 916

Query: 744  HIVFYKHFDVPLGKFLQDNLLNQNNLCTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKV 803
             I +Y  FD PLG+FL+D+L +Q+  C  C    EAH + Y H +  L+I VK+L  D +
Sbjct: 917  RIKYYGSFDKPLGRFLRDHLFDQSYRCRSCEMPSEAHVHCYTHRQGSLTISVKKLQ-DYL 976

Query: 804  LPGETEGKLWMWSRCSKCKSTGGPSKSTKRVLISTAARGLSFGKFLELCFSDDTLLGKSS 863
            LPGE EGK+WMW RC +C    G   +T RV++S AA GLSFGKFLEL FS+     + +
Sbjct: 977  LPGEKEGKIWMWHRCLRCPRLNGFPPATLRVVMSDAAWGLSFGKFLELSFSNHAAASRVA 1036

Query: 864  VCGHSLFGDFLYFFGLGNMVAMSRYSEVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYT 923
             CGHSL  D L F+G GNMVA  RY+ + +++V +PP  L FN    Q  + RET+ V  
Sbjct: 1037 CCGHSLHRDCLRFYGFGNMVACFRYATIDVHSVYLPPSILSFNYE-NQDWIQRETDEVIE 1096

Query: 924  KGVLLFTEIAHSLKKIISERKSSNVNHPQCFPNDFSFVEEMLNEERSEFELNIQNSL--- 983
            +  LLF+E+ +++ +I  +           F      +EE+L +E++EFE N+Q  L   
Sbjct: 1097 RAELLFSEVLNAISQIAEKG----------FRRRIGELEEVLQKEKAEFEENMQKILHRE 1156

Query: 984  TKKGNP--DFLNLNRLLWDLLLESCIWDRRLQSLRSLGVTTNSGSSEIVEPE-PLLLKMN 1043
              +G P  D L L R+   LL +S +WD RL +  +L    NS  ++  E E P L K  
Sbjct: 1157 VNEGQPLVDILELYRIHRQLLFQSYMWDHRLINASTLHKLENSDDTKREENEKPPLAKSQ 1216

Query: 1044 S--NIDVGPEELESTAE---NDDTKVQQDISLEENVLPLKEITVEGLDGESGGDELNLPS 1103
            +   ++ G   L + +E   N D     D     NV    +   +    +  G E++   
Sbjct: 1217 TLPEMNAGTNSLLTGSEVNLNPDGDSTGDTGSLNNVQKEADTNSDLYQEKDDGGEVSPSK 1276

Query: 1104 AIEVTEIPIIDDLSPKKLSRQGTL-------------------------SNGFDYHHSDY 1163
             +  T  P+ + +  ++    G +                         +N      S  
Sbjct: 1277 TLPDTSYPLENKVDVRRTQSDGQIVMKNLSATLDAAWIGERQTSVEIPTNNKVSLPPSTM 1336

Query: 1164 EDSQVGKVLSSGDLHID---------------RTIPISIGDSTLSKLFW--TPFSEIRQM 1223
             +S     +S G + ID                 +P    +++   + W   PF      
Sbjct: 1337 SNSSTFPPISEGLMPIDLPEQQNEFKVAYPVSPALPSKNYENSEDSVSWLSVPFLNF--- 1396

Query: 1224 HLRDIQRSYF---PELKSISSYTPKLLPAASDFIHEEGQKLHIHLDNDNFVVSDYEGELS 1283
              R I +++     +L +   ++P  + +  +   + G +L + +  ++ VV  Y+ E +
Sbjct: 1397 -YRSINKNFLLSSQKLDTFGEHSPIYISSFREAELQGGPRLLLPVGLNDIVVPVYDDEPT 1456

Query: 1284 SIVACVLALLKDQPFQTDSHNEDSKGEGGGALQPALSSRSLNKVPSNGSSDSDSSISSEE 1343
            S++A  L +  +   QT +  E           P     ++   PS  +   D SI S  
Sbjct: 1457 SMIAYAL-MSPEYQRQTSAEGESLVSYPSELNIPRPVDDTIFD-PSRSNGSVDESILS-- 1516

Query: 1344 YRFSSFDRLNLLDSL-VSETFKRSDHEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSEL 1403
               SS    +LLD L  ++        G   +L K KY V C Y  +F  LR  C PSEL
Sbjct: 1517 --ISSSRSTSLLDPLSYTKALHARVSYGEDGTLGKVKYTVTCYYAKRFEALRGICLPSEL 1576

Query: 1404 HFIASLSRCINWNAKGGKSKSFFAKTLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFD 1463
             +I SLSRC  W A+GGKS  FFAKTLDDRFIIK++ +TE +SF+KFAP YFKY++ES  
Sbjct: 1577 EYIRSLSRCKKWGAQGGKSNVFFAKTLDDRFIIKQVTKTELESFIKFAPAYFKYLSESIS 1636

Query: 1464 MGNQTCLAKVLGIYQVTVREPKSGKEMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPA 1523
              + TCLAK+LGIYQV  ++ KSGKE + D+++MENL FGR + R YDLKG+  AR+NP 
Sbjct: 1637 TKSPTCLAKILGIYQVATKQLKSGKETKMDVLIMENLLFGRTVKRLYDLKGSSRARYNPD 1696

Query: 1524 TNNFGEVLLDQNFVNDMNSSPLYVSNRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAE 1545
            ++   +VLLDQN +  M +SP++V N+AKR L+RA+WNDTAFL   +VMDYSLLVGVD E
Sbjct: 1697 SSGSNKVLLDQNLIEAMPTSPIFVGNKAKRLLERAVWNDTAFLALGDVMDYSLLVGVDEE 1756

BLAST of Chy1G009970 vs. TAIR 10
Match: AT4G33240.1 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 740.0 bits (1909), Expect = 4.1e-213
Identity = 531/1481 (35.85%), Postives = 768/1481 (51.86%), Query Frame = 0

Query: 155  LWDPPEPEDPEDHMEGGMGYNDDDDDEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRA 214
            LW PPEPE+ ED  E  +  +DDD DE GD  +W      S S +E    +  K++   A
Sbjct: 311  LWLPPEPENEEDEREAVL--SDDDGDE-GDRGDWGYLRP-SNSFNEK--DFHSKDKSSGA 370

Query: 215  LQQVMNGKHKAFIRDLLKLGE-------DSENWVDIVSSLSWEAATFLKP-VVNGKAMDP 274
            ++ V+ G  +A +  LL++         D E W+DI++SLSWEAAT LKP       MDP
Sbjct: 371  MKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMDP 430

Query: 275  DANVKVKCIATGTRNQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPIS-RLS 334
               VKVKCI  G R++S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +LS
Sbjct: 431  GGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLS 490

Query: 335  SFNS-MDQENDFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQR 394
            SF++ + QE D     +  I+    +++LVEK+ +R  QE +L K ++LVL++K   L+R
Sbjct: 491  SFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLER 550

Query: 395  IALCTGSPILTS-ETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCSTRL 454
            I+ CTG+ I+ S + L S K   CD  + +K VE H    +  K   KTLMF +GC   L
Sbjct: 551  ISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPL 610

Query: 455  GCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSD 514
            GCTILLKGAH DELK+VK V+Q  V  A+HL LETSF+ D+ A    +P    P T +  
Sbjct: 611  GCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVALP 670

Query: 515  PQSPLEPRSPNVGKAESASDKSEQPTS---------------IYISRGSHEEASTGSN-- 574
             +  +  RS +     + S   + PT+                + S  +H +     N  
Sbjct: 671  DKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGNDR 730

Query: 575  MELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQSLSSYFGHGSDLSG 634
            ++ +E+L+ + +     P      +S  D L   + ES   S   +  S      S LSG
Sbjct: 731  IDPSERLLHNLDTVYCKPP--ETITSKDDGLVPTL-ESRQLSFHVEEPSVQKDQWSVLSG 790

Query: 635  LIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSHQSTLEGL--GFHETAPNYSVD 694
                     +T Q      V   G +++   +  Q+ + Q  +E     FH +A      
Sbjct: 791  ---------ATEQ------VTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHPSAS----- 850

Query: 695  TMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLGKFLQDNLLNQNNL 754
                    D QSILV +S+R   KG++CE++H   I +Y  FD PLG+FL+DNL +Q+  
Sbjct: 851  --------DHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQC 910

Query: 755  CTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSTGGPSK 814
            C  C    EAH + Y H +  L+I VK+LP  ++LPG+ EGK+WMW RC KC    G   
Sbjct: 911  CPSCTMPAEAHIHCYTHRQGSLTISVKKLP--ELLPGQREGKIWMWHRCLKCPRINGFPP 970

Query: 815  STKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYFFGLGNMVAMSRYS 874
            +T+R+++S AA GLSFGKFLEL FS+     + + CGHSL  D L F+G G MVA  RY+
Sbjct: 971  ATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYA 1030

Query: 875  EVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSLKKIISERKSSNVN 934
             + IY V++PP KL FN    Q  L +E++ V  K  +LF E+  +L +I    K+    
Sbjct: 1031 SINIYAVTLPPAKLYFNYE-NQEWLQKESKEVIKKAEVLFNEVQEALSQI--SAKTMGAG 1090

Query: 935  HPQCFPNDFSF-VEE---MLNEERSEFELNIQNSL--TKKGNP--DFLNLNRLLWDLLLE 994
                 PN     +EE   +L + + E++ ++Q  L   K G P  D L +N+L   ++ +
Sbjct: 1091 SKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQMLNVVKDGQPTIDILLINKLRRLIIFD 1150

Query: 995  SCIWDRRLQSLRSL-------------------GVTTNSGSSEIVEPEPLLLKMNSNIDV 1054
            S  WD  L    ++                    V+    S E V+  P  + + ++  +
Sbjct: 1151 SYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAICNDSLL 1210

Query: 1055 GPEELESTAEND----DTKVQQDISLEENVLPLKEITVEGLDGESGGDEL---------- 1114
               + E+         DT  +  I  +       +  +E    E G D            
Sbjct: 1211 QDADYETCLNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDPSEGGKDNFVESSQVVKPA 1270

Query: 1115 NLPSAIEVTEIPIIDDLSPKKLSRQGTLSNGFDYHHSDYEDSQVGKV------------- 1174
            +  S  + T++   D L    +  Q T  NG     S    +   ++             
Sbjct: 1271 HTESQFQATDLS--DTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIPDLRLLGSESELN 1330

Query: 1175 LSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLP 1234
               G  + + T  + +   +        +S   + H+    R  +     +SSY      
Sbjct: 1331 FKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKHIMAEDRPVY-----VSSY------ 1390

Query: 1235 AASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEG 1294
               +     G +L + L  ++ V+  Y+ E +SI+A  L   + +   + S     + + 
Sbjct: 1391 --RELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSGSDKSRDRLDS 1450

Query: 1295 GG--ALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFSSF--DRLNLLDSLVSETFKRSD 1354
            GG  +L  +++  SLN + S+ S D   S+SS + + S      L L D     +F    
Sbjct: 1451 GGSFSLFDSVNLLSLNSL-SDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEG 1510

Query: 1355 HEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAK 1414
              G      K KY V C Y  +F  LR  CCPSE  FI SL RC  W A+GGKS  FFAK
Sbjct: 1511 PPG------KVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAK 1570

Query: 1415 TLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREPKSGK 1474
            +LDDRFIIK++ +TE +SF+KF P YFKY+ ES    + T LAK+LGIYQV+ +  K GK
Sbjct: 1571 SLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGK 1630

Query: 1475 EMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVS 1534
            E + D++VMENL F RN  R YDLKG+  AR+NP T+    VLLDQN V  M +SP++V 
Sbjct: 1631 EFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSPIFVG 1690

Query: 1535 NRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWV 1545
            ++AKR L+RA+WNDT+FL SI+VMDYSLLVGVD E+ ELV GIID++RQYTWDK LETWV
Sbjct: 1691 SKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHLETWV 1726

BLAST of Chy1G009970 vs. TAIR 10
Match: AT4G33240.2 (1-phosphatidylinositol-4-phosphate 5-kinases;zinc ion binding;1-phosphatidylinositol-3-phosphate 5-kinases )

HSP 1 Score: 740.0 bits (1909), Expect = 4.1e-213
Identity = 531/1481 (35.85%), Postives = 768/1481 (51.86%), Query Frame = 0

Query: 155  LWDPPEPEDPEDHMEGGMGYNDDDDDEFGDNSEWSTASSFSRSVDEASVSYRFKEEKQRA 214
            LW PPEPE+ ED  E  +  +DDD DE GD  +W      S S +E    +  K++   A
Sbjct: 310  LWLPPEPENEEDEREAVL--SDDDGDE-GDRGDWGYLRP-SNSFNEK--DFHSKDKSSGA 369

Query: 215  LQQVMNGKHKAFIRDLLKLGE-------DSENWVDIVSSLSWEAATFLKP-VVNGKAMDP 274
            ++ V+ G  +A +  LL++         D E W+DI++SLSWEAAT LKP       MDP
Sbjct: 370  MKNVVEGHFRALVAQLLEVDNLPMVNEGDEEGWLDIITSLSWEAATLLKPDTSKSGGMDP 429

Query: 275  DANVKVKCIATGTRNQSQFVKGMVFKKHAAHKHMPTHCKNPKLILIQGMLGEAPIS-RLS 334
               VKVKCI  G R++S  VKG+V KK+ AH+ M +  + P+L+++ G L    IS +LS
Sbjct: 430  GGYVKVKCIPCGRRSESMVVKGVVCKKNVAHRRMTSKIEKPRLLILGGALEYQRISNQLS 489

Query: 335  SFNS-MDQENDFTNHVIEMIEGCTANVILVEKTAARVIQEAILKKGMTLVLDMKLHRLQR 394
            SF++ + QE D     +  I+    +++LVEK+ +R  QE +L K ++LVL++K   L+R
Sbjct: 490  SFDTLLQQEMDHLKMAVAKIDSHNPDILLVEKSVSRFAQEYLLAKDISLVLNIKRSLLER 549

Query: 395  IALCTGSPILTS-ETLMSQKTRQCDAVYFQKIVEEHAGVLEGGKMPTKTLMFIEGCSTRL 454
            I+ CTG+ I+ S + L S K   CD  + +K VE H    +  K   KTLMF +GC   L
Sbjct: 550  ISRCTGAQIVPSIDQLTSPKLGYCDLFHVEKFVETHVSPCQVAKKMAKTLMFFDGCPKPL 609

Query: 455  GCTILLKGAHSDELKRVKIVVQCAVVMAFHLILETSFIVDQRAMFATIPFGGVPATASSD 514
            GCTILLKGAH DELK+VK V+Q  V  A+HL LETSF+ D+ A    +P    P T +  
Sbjct: 610  GCTILLKGAHEDELKKVKHVIQYGVFAAYHLALETSFLADEGASIHELPL-QTPITVALP 669

Query: 515  PQSPLEPRSPNVGKAESASDKSEQPTS---------------IYISRGSHEEASTGSN-- 574
             +  +  RS +     + S   + PT+                + S  +H +     N  
Sbjct: 670  DKPSMVNRSISTIPGFTVSSAEKSPTTELRGEPHKANGDLTGNFTSSKTHFQGKLDGNDR 729

Query: 575  MELAEKLIMSSEPEPYNPAIFSGFSSISDSLKRVMGESFLSSSPYQSLSSYFGHGSDLSG 634
            ++ +E+L+ + +     P      +S  D L   + ES   S   +  S      S LSG
Sbjct: 730  IDPSERLLHNLDTVYCKPP--ETITSKDDGLVPTL-ESRQLSFHVEEPSVQKDQWSVLSG 789

Query: 635  LIAKSSSIPSTPQAVYQFDVEVRGSSDEENSVHEQSVSHQSTLEGL--GFHETAPNYSVD 694
                     +T Q      V   G +++   +  Q+ + Q  +E     FH +A      
Sbjct: 790  ---------ATEQ------VTDGGYTNDSAVIGNQNFNRQEQMESSKGDFHPSAS----- 849

Query: 695  TMQKKMSLDSQSILVLMSSRNALKGAMCEQSHFSHIVFYKHFDVPLGKFLQDNLLNQNNL 754
                    D QSILV +S+R   KG++CE++H   I +Y  FD PLG+FL+DNL +Q+  
Sbjct: 850  --------DHQSILVSLSTRCVWKGSVCERAHLLRIKYYGSFDKPLGRFLRDNLFDQDQC 909

Query: 755  CTVCGGLPEAHFYFYAHYRKQLSIQVKQLPGDKVLPGETEGKLWMWSRCSKCKSTGGPSK 814
            C  C    EAH + Y H +  L+I VK+LP  ++LPG+ EGK+WMW RC KC    G   
Sbjct: 910  CPSCTMPAEAHIHCYTHRQGSLTISVKKLP--ELLPGQREGKIWMWHRCLKCPRINGFPP 969

Query: 815  STKRVLISTAARGLSFGKFLELCFSDDTLLGKSSVCGHSLFGDFLYFFGLGNMVAMSRYS 874
            +T+R+++S AA GLSFGKFLEL FS+     + + CGHSL  D L F+G G MVA  RY+
Sbjct: 970  ATRRIVMSDAAWGLSFGKFLELSFSNHAAASRVANCGHSLHRDCLRFYGFGRMVACFRYA 1029

Query: 875  EVAIYTVSMPPQKLEFNSSMRQGHLIRETENVYTKGVLLFTEIAHSLKKIISERKSSNVN 934
             + IY V++PP KL FN    Q  L +E++ V  K  +LF E+  +L +I    K+    
Sbjct: 1030 SINIYAVTLPPAKLYFNYE-NQEWLQKESKEVIKKAEVLFNEVQEALSQI--SAKTMGAG 1089

Query: 935  HPQCFPNDFSF-VEE---MLNEERSEFELNIQNSL--TKKGNP--DFLNLNRLLWDLLLE 994
                 PN     +EE   +L + + E++ ++Q  L   K G P  D L +N+L   ++ +
Sbjct: 1090 SKGSTPNKIKLSLEELAGLLEQRKKEYKDSLQQMLNVVKDGQPTIDILLINKLRRLIIFD 1149

Query: 995  SCIWDRRLQSLRSL-------------------GVTTNSGSSEIVEPEPLLLKMNSNIDV 1054
            S  WD  L    ++                    V+    S E V+  P  + + ++  +
Sbjct: 1150 SYAWDECLAGAANMVRNNYLEAPKNSAPKVMGRNVSLEKLSDEKVKSIPTHVAICNDSLL 1209

Query: 1055 GPEELESTAEND----DTKVQQDISLEENVLPLKEITVEGLDGESGGDEL---------- 1114
               + E+         DT  +  I  +       +  +E    E G D            
Sbjct: 1210 QDADYETCLNQGKSFADTSGKFAIPEDVGSDRPPDCRMEFDPSEGGKDNFVESSQVVKPA 1269

Query: 1115 NLPSAIEVTEIPIIDDLSPKKLSRQGTLSNGFDYHHSDYEDSQVGKV------------- 1174
            +  S  + T++   D L    +  Q T  NG     S    +   ++             
Sbjct: 1270 HTESQFQATDLS--DTLDAAWIGEQTTSENGIFRPPSRAASTNGTQIPDLRLLGSESELN 1329

Query: 1175 LSSGDLHIDRTIPISIGDSTLSKLFWTPFSEIRQMHLRDIQRSYFPELKSISSYTPKLLP 1234
               G  + + T  + +   +        +S   + H+    R  +     +SSY      
Sbjct: 1330 FKGGPTNDEHTTQVQLPSPSFYYSLNKNYSLNSRKHIMAEDRPVY-----VSSY------ 1389

Query: 1235 AASDFIHEEGQKLHIHLDNDNFVVSDYEGELSSIVACVLALLKDQPFQTDSHNEDSKGEG 1294
               +     G +L + L  ++ V+  Y+ E +SI+A  L   + +   + S     + + 
Sbjct: 1390 --RELEWRSGARLLLPLGCNDLVLPVYDDEPTSIIAYALTSSEYKAQMSGSDKSRDRLDS 1449

Query: 1295 GG--ALQPALSSRSLNKVPSNGSSDSDSSISSEEYRFSSF--DRLNLLDSLVSETFKRSD 1354
            GG  +L  +++  SLN + S+ S D   S+SS + + S      L L D     +F    
Sbjct: 1450 GGSFSLFDSVNLLSLNSL-SDLSVDMSRSLSSADEQVSQLLHSSLYLKDLHARISFTDEG 1509

Query: 1355 HEGVIKSLAKGKYLVNCPYINQFRDLRSRCCPSELHFIASLSRCINWNAKGGKSKSFFAK 1414
              G      K KY V C Y  +F  LR  CCPSE  FI SL RC  W A+GGKS  FFAK
Sbjct: 1510 PPG------KVKYSVTCYYAKEFEALRMICCPSETDFIRSLGRCRKWGAQGGKSNVFFAK 1569

Query: 1415 TLDDRFIIKEIKRTEYDSFMKFAPEYFKYINESFDMGNQTCLAKVLGIYQVTVREPKSGK 1474
            +LDDRFIIK++ +TE +SF+KF P YFKY+ ES    + T LAK+LGIYQV+ +  K GK
Sbjct: 1570 SLDDRFIIKQVTKTELESFIKFGPAYFKYLTESISTKSPTSLAKILGIYQVSSKHLKGGK 1629

Query: 1475 EMRHDLMVMENLSFGRNIIRQYDLKGALHARFNPATNNFGEVLLDQNFVNDMNSSPLYVS 1534
            E + D++VMENL F RN  R YDLKG+  AR+NP T+    VLLDQN V  M +SP++V 
Sbjct: 1630 EFKMDVLVMENLLFKRNFTRLYDLKGSTRARYNPDTSGSNTVLLDQNLVEAMPTSPIFVG 1689

Query: 1535 NRAKRWLQRAIWNDTAFLNSINVMDYSLLVGVDAEKKELVCGIIDYLRQYTWDKQLETWV 1545
            ++AKR L+RA+WNDT+FL SI+VMDYSLLVGVD E+ ELV GIID++RQYTWDK LETWV
Sbjct: 1690 SKAKRLLERAVWNDTSFLASIHVMDYSLLVGVDEERNELVLGIIDFMRQYTWDKHLETWV 1725