Chy1G006930 (gene) Cucumber (hystrix) v1

Overview


Name	Chy1G006930
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	Amidase domain-containing protein
Location	chrH01: 5063813 .. 5067044 (-)
RNA-Seq Expression	Chy1G006930
Synteny	Chy1G006930

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACATCGTCTTCTTCTTCTTCTTCTTCCTCTCAGCTGTCTTACTCTTCACCGGCGTCGCCAACTCCTCCCACTTCTCCATCGACGAAGCCACCATTGCCGAAATTCAAAACGCCTTCTCCCAAAACAAACTCACCTCCACACAACTCCTCGACTATTACCTCAAAAAAATACACCTCCTCAATCCAGTCCTCAAAAGCGTTCTCGAACTAAACCCAGACGCAAGAGCTCAAGCGGAAGCCGCCGATCGAGAGAGGCTCCTCGCCGGTGGAAAAGCCCTAGGCGAACTCCATGGAATTCCAATTCTACTCAAGGACGCCATTGCGACTAAGGATCTTCTCAATACAACCGCCGGATCCTTCGCGTTGTTGGGTTCGGTGGTGCCTCGAGATGCGACGGTGGTTAGTCGCCTGAGAAACGCCGGTGCAGTGATTTTGGGGAAAACTTCACTTACAGAATGGTATAAATCCCGTTCTTATGAGATTCCCAATGGATGGTGTGCTCGTGGCGGTCAAGCGGTGGTTAGTAAATTTTTTTCCCCCCAAGACTTTTTGCCCTCGAATTGAATTGAATTGAATTGAATTGAATGAAATACACAGAACCCATATGGAAAAGGAGGGGATCCATGTGGGTCAAGCAGTGGGTCGGCGATATCGGTGGCCGCGAATATGGTGGCGGTGTCATTAGGGACGGAGACTGATGGTTCGATTCTGTGTCCGGCCGATTACAACTCGGTGGTTGGGATTAAGCCTACCGTCGGTCTCACGAGCCGGGCCGGCGTTATCCCGGTCACTCCTCGCCAAGACACAATTGGGTAATACAAACTTGAATGAATGAACCCTACAAATTTGGAATATTTTTTACATTCTAACCTAATAAATTACTTCTAATGAAATTTCAATCATTTTAAGTATATATACTCTCAAATTTGTTCGCTTTTAGTTTAGATAGCTTTAGTTGTCCAATTTTAATCTATATAGGTTTAATATTCAATTAAAAAAGAAAAGTTACCTTTTTAATGTTTCTCTCAATTTTCATTACAAGTTTGATTAGAAGGTGCAATTGAATTGATGGGGGAGTTGACAGGCCCATATGTAGAACAGTTTCAGATGCTGTTTATGTGCTTGAAGCTATTGTTGGATTTGATCCGATGGACTATGAGGTAACCAAGGAAGCTTCTCAATTCATACCTCCAGGTGGTTACAAACAGTTTCTAAGGAAAGATGGGCTCAAGGGAAAGAGATTAGGCATTGTTAGGCATCCCTTTTCTGACTTGTATCCCAATAACTCCATGGCCATTCCAACTTTTGAGCAACATCTCAATCTGTTAAGGTATATTTGCAAAAATAATCACTTCTTTCTTCTTTTTTGGTCCACATATAGTTTGATTTGGTTCTTTACCATTTAGAATTTAGAATTGAGTTCTTATACTATGAGATTTTTTTAGGTGAAATTACAAAAAGTACCACTGAACTTTACTTTTTTGTTTTAAAGGACGTGCTCTTGTTGTTTCAAAAATTGTAATGTTACTTTTCATTGTAGAAAGTTATTAGAATGTTGGGATGAAAATTGAGACGGTTTGGTAGTAATTTGGACACCTCTACACTTGCTCCATGTCTCCATTTTCATCCTAAGTTTTATTGGATTACTACTGCAGGAGTAGTTTGAATCATTTTTGAAAAACCAAAGGTAATGATGTTGTAAACCAAAGTTAATGTTGTAACTTCTAAATAATAGGAGTATTGTCTAAACATAGAGCAACAGAGCAAATTTCGAAAGGTTTTATCAACGATTAGGTGTTGTGTATATTAGTTTTGAGTAAATTGAAAAAATTACTCACAAAGTTTGTTGATAGTTGTAATTGTACTTTTACTCTCAATTGTAAAAAATTTAGCCTCCAAACTTGTACAAGTATCACACTTGACCGTCAAACTTGCAAAGTTGTAAGTATAACAACCATTTAAGCTTAAGGATTTAATTCTTATCATTTGGGGATTCGATTTCTACAACTATTGTAAGTTCAAGGATTCAATTTTAACACTTGTATAAATAAGTTTGAATGTTTAATTTTTTCTATTAAAAGTTTAAAAGTGTAATTACAACGATTTTAGGAGTTATTTTTGTAATTTGACCATTAGTTTTTGAAGTGTATTTGGTGTATCATACTAGTTGCTGAGAGTTATTTATTTAATTTAATTTTTTCTTGGCTGAAACTGAAGGAAGATGGGAGCAACCATTGTAGACAACCTTCAAATATCAAATGTAGATGTCATTTTGAATTCGTATGAAAGTGGTGAATTCATTGCAATAATAGCTGAGTTCAAGGTGGCCTTAAATGATTACCTCAAGAAACTCATCCGAAGTCCAGTTAAATCTTTGGCTGACATTATCTCCTTCAACAACAACCACCCTGAACTAGTAAGACTTTCTTATGACTCGAATCATACAATAAAAGTATAACATTATGTTTTTTTATGGTGTCTTACTTGACTCTTAAAAGAAGAGTTTTCTTTTTGTTTTGTACAAATGACCCTTCCATAGATCCCTGTATAATTAATGATTTGTCCCCTACAAATGTGTTAAGTCTAACATTATTACGTTAGAGAGCACAATCGCAACAAAACAAAATTGAAAGGTTGCGATGCATTATAAAATCTGATAATGGGCTGAAAGTTAGATTCCATGCATTTGTACGGAGCGGGTCACTAGTCTTGAACAAGTCTATGAAAGAGTCATCCGTACAGAAACTCCTTAGAGAAGTGTAGAGTATATTATAAGTAACATTTCAAAAACGTCAAGTAACACAATTATTCTATTCTTTGATAAGGTGTAAGTTTGTATTTCTTGTTGGGATTGCAGGAGAAAATGAAAGAGTATGGCCAAGATGCCTTTCTTTTATCTGAACAAACAAATGGTATCGGAGTGATGGAGAAGGAGGCGATTAGTAAGATGGCGAACTTATCACGAAACGGGTTTGAGGAATTAATGAAAGGCAACAATTTGGATGCAATGGTGACAATAGGGATAGGAGTTGAAAGTGTGCTAGCAATTGGGGGATACCCAGGAATAAGTGTCCCTGCTGGATATGAAGAAAATGGAGAGCCATTTGGTATTCTATTTGGAGGTCTTAAGGGGAGTGAACCAAAGCTAATTGAGATTGCTTATGCATATGAACAAGCCACCATGGTGAGAGAGCCACCTCCTCTTCTTTCTCAAATTTTAAGTTTGTGA

mRNA sequence

ATGAACATCGTCTTCTTCTTCTTCTTCTTCCTCTCAGCTGTCTTACTCTTCACCGGCGTCGCCAACTCCTCCCACTTCTCCATCGACGAAGCCACCATTGCCGAAATTCAAAACGCCTTCTCCCAAAACAAACTCACCTCCACACAACTCCTCGACTATTACCTCAAAAAAATACACCTCCTCAATCCAGTCCTCAAAAGCGTTCTCGAACTAAACCCAGACGCAAGAGCTCAAGCGGAAGCCGCCGATCGAGAGAGGCTCCTCGCCGGTGGAAAAGCCCTAGGCGAACTCCATGGAATTCCAATTCTACTCAAGGACGCCATTGCGACTAAGGATCTTCTCAATACAACCGCCGGATCCTTCGCGTTGTTGGGTTCGGTGGTGCCTCGAGATGCGACGGTGGTTAGTCGCCTGAGAAACGCCGGTGCAGTGATTTTGGGGAAAACTTCACTTACAGAATGGTATAAATCCCGTTCTTATGAGATTCCCAATGGATGGTGTGCTCGTGGCGGTCAAGCGGTGAACCCATATGGAAAAGGAGGGGATCCATGTGGGTCAAGCAGTGGGTCGGCGATATCGGTGGCCGCGAATATGGTGGCGGTGTCATTAGGGACGGAGACTGATGGTTCGATTCTGTGTCCGGCCGATTACAACTCGGTGGTTGGGATTAAGCCTACCGTCGGTCTCACGAGCCGGGCCGGCGTTATCCCGGTCACTCCTCGCCAAGACACAATTGGGCCCATATGTAGAACAGTTTCAGATGCTGTTTATGTGCTTGAAGCTATTGTTGGATTTGATCCGATGGACTATGAGGTAACCAAGGAAGCTTCTCAATTCATACCTCCAGGTGGTTACAAACAGTTTCTAAGGAAAGATGGGCTCAAGGGAAAGAGATTAGGCATTGTTAGGCATCCCTTTTCTGACTTGTATCCCAATAACTCCATGGCCATTCCAACTTTTGAGCAACATCTCAATCTGTTAAGGAAGATGGGAGCAACCATTGTAGACAACCTTCAAATATCAAATGTAGATGTCATTTTGAATTCGTATGAAAGTGGTGAATTCATTGCAATAATAGCTGAGTTCAAGGTGGCCTTAAATGATTACCTCAAGAAACTCATCCGAAGTCCAGTTAAATCTTTGGCTGACATTATCTCCTTCAACAACAACCACCCTGAACTAGAGAAAATGAAAGAGTATGGCCAAGATGCCTTTCTTTTATCTGAACAAACAAATGGTATCGGAGTGATGGAGAAGGAGGCGATTAGTAAGATGGCGAACTTATCACGAAACGGGTTTGAGGAATTAATGAAAGGCAACAATTTGGATGCAATGGTGACAATAGGGATAGGAGTTGAAAGTGTGCTAGCAATTGGGGGATACCCAGGAATAAGTGTCCCTGCTGGATATGAAGAAAATGGAGAGCCATTTGGTATTCTATTTGGAGGTCTTAAGGGGAGTGAACCAAAGCTAATTGAGATTGCTTATGCATATGAACAAGCCACCATGGTGAGAGAGCCACCTCCTCTTCTTTCTCAAATTTTAAGTTTGTGA

Coding sequence (CDS)

ATGAACATCGTCTTCTTCTTCTTCTTCTTCCTCTCAGCTGTCTTACTCTTCACCGGCGTCGCCAACTCCTCCCACTTCTCCATCGACGAAGCCACCATTGCCGAAATTCAAAACGCCTTCTCCCAAAACAAACTCACCTCCACACAACTCCTCGACTATTACCTCAAAAAAATACACCTCCTCAATCCAGTCCTCAAAAGCGTTCTCGAACTAAACCCAGACGCAAGAGCTCAAGCGGAAGCCGCCGATCGAGAGAGGCTCCTCGCCGGTGGAAAAGCCCTAGGCGAACTCCATGGAATTCCAATTCTACTCAAGGACGCCATTGCGACTAAGGATCTTCTCAATACAACCGCCGGATCCTTCGCGTTGTTGGGTTCGGTGGTGCCTCGAGATGCGACGGTGGTTAGTCGCCTGAGAAACGCCGGTGCAGTGATTTTGGGGAAAACTTCACTTACAGAATGGTATAAATCCCGTTCTTATGAGATTCCCAATGGATGGTGTGCTCGTGGCGGTCAAGCGGTGAACCCATATGGAAAAGGAGGGGATCCATGTGGGTCAAGCAGTGGGTCGGCGATATCGGTGGCCGCGAATATGGTGGCGGTGTCATTAGGGACGGAGACTGATGGTTCGATTCTGTGTCCGGCCGATTACAACTCGGTGGTTGGGATTAAGCCTACCGTCGGTCTCACGAGCCGGGCCGGCGTTATCCCGGTCACTCCTCGCCAAGACACAATTGGGCCCATATGTAGAACAGTTTCAGATGCTGTTTATGTGCTTGAAGCTATTGTTGGATTTGATCCGATGGACTATGAGGTAACCAAGGAAGCTTCTCAATTCATACCTCCAGGTGGTTACAAACAGTTTCTAAGGAAAGATGGGCTCAAGGGAAAGAGATTAGGCATTGTTAGGCATCCCTTTTCTGACTTGTATCCCAATAACTCCATGGCCATTCCAACTTTTGAGCAACATCTCAATCTGTTAAGGAAGATGGGAGCAACCATTGTAGACAACCTTCAAATATCAAATGTAGATGTCATTTTGAATTCGTATGAAAGTGGTGAATTCATTGCAATAATAGCTGAGTTCAAGGTGGCCTTAAATGATTACCTCAAGAAACTCATCCGAAGTCCAGTTAAATCTTTGGCTGACATTATCTCCTTCAACAACAACCACCCTGAACTAGAGAAAATGAAAGAGTATGGCCAAGATGCCTTTCTTTTATCTGAACAAACAAATGGTATCGGAGTGATGGAGAAGGAGGCGATTAGTAAGATGGCGAACTTATCACGAAACGGGTTTGAGGAATTAATGAAAGGCAACAATTTGGATGCAATGGTGACAATAGGGATAGGAGTTGAAAGTGTGCTAGCAATTGGGGGATACCCAGGAATAAGTGTCCCTGCTGGATATGAAGAAAATGGAGAGCCATTTGGTATTCTATTTGGAGGTCTTAAGGGGAGTGAACCAAAGCTAATTGAGATTGCTTATGCATATGAACAAGCCACCATGGTGAGAGAGCCACCTCCTCTTCTTTCTCAAATTTTAAGTTTGTGA

Protein sequence

MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHLLNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGSFALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKGGDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLGIVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIAEFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKEAISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGILFGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL*

Homology

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BLAST of Chy1G006930 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 2.8e-163
Identity = 312/519 (60.12%), Postives = 393/519 (75.72%), Query Frame = 0


Query: 5   FFFFFFL-------SAVLLFTGVAN----SSHFSIDEATIAEIQNAFSQNKLTSTQLLDY 64
           FFFF  L        A+++  G A+    SS FSI EATI +I+ AF++ +LTS QL++ 
Sbjct: 6   FFFFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVEL 65

Query: 65  YLKKIHLLNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDL 124
           YL+ I  LNP+L +V+E NPDA  QAE ADRER L     L  LHG+P+LLKD+I+TKD 
Sbjct: 66  YLEAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDK 125

Query: 125 LNTTAGSFALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQA 184
           LNTTAGSFALLGSVV RDA VV RLR +GAVILGK SL+EW   RS+ IP+GW ARG Q 
Sbjct: 126 LNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQG 185

Query: 185 VNPYGKGGDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRA 244
            NPY    +P GSSSGSAISV AN+VAVSLGTETDGSIL PA  NSVVGIKP+VGLTSRA
Sbjct: 186 KNPYVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRA 245

Query: 245 GVIPVTPRQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDG 304
           GV+P++ RQD+IGPICRTVSDAV++L+AIVG+DP+D E TK AS+FIP GGYKQFL   G
Sbjct: 246 GVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLD-EATKTASEFIPEGGYKQFLTTSG 305

Query: 305 LKGKRLGIVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESG 364
           LKGKRLGIV    S L           + H+  LR+ GA +++NL I N++VI+   +SG
Sbjct: 306 LKGKRLGIVMKHSSLL-----------DHHIKTLRREGAIVINNLTIPNIEVIVGGTDSG 365

Query: 365 EFIAIIAEFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNG 424
           E IA++AEFK++LN YLK+L++SPV+SLAD+I++N    E EK+KE+GQ+ FL +E T+G
Sbjct: 366 EEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSG 425

Query: 425 IGVMEKEAISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEEN 484
           +G  EK A+ KM  LSRNG E+L++ N LDA+VT+G  + SVLAIGGYPGI+VPAGY+  
Sbjct: 426 MGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSG 485

Query: 485 GEPFGILFGGLKGSEPKLIEIAYAYEQATMVREPPPLLS 513
           G P+GI FGGL+ SEPKLIEIA+A+EQAT++R+PP  ++
Sbjct: 486 GVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPPKFIA 512

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BLAST of Chy1G006930 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 5.3e-82
Identity = 198/513 (38.60%), Postives = 283/513 (55.17%), Query Frame = 0


Query: 25  HFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHLLNPVLKSVLELNPDARAQAEAADR 84
           + ++++ATI ++QN      LTST ++  YL +   +NP +  +L+LNPD    A   D 
Sbjct: 63  NITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDD 122

Query: 85  ERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGSFALLGSVVPRDATVVSRLRNAGAV 144
           ER  A G   G LHGIP ++KD  ATKD ++TTAGS+ALLGS+VPRDA VV +LR AGAV
Sbjct: 123 ER--ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAV 182

Query: 145 ILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKGGDPCGSSSGSAISVAANMVAVSLG 204
           + G  +L+EW   RS +   G+ ARGGQ+  P+    +P GSSSGSAISVA+NM+A +LG
Sbjct: 183 LFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALG 242

Query: 205 TETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYVLEAIVG 264
           TETDGSI+ PA  N VVG+KPTVGLTSR GVIP +  QDT GPI RTV DAVYV +++ G
Sbjct: 243 TETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWG 302

Query: 265 FDPMDYEVTKEASQFIPPGGYKQFL-RKDGLKGKRLGIVRHPFSDLYPN-NSMAIPTFEQ 324
            D  D     +  +    G Y +FL  K  L+G R G+   P+  L+ N  +  I    +
Sbjct: 303 IDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGL---PWKRLWQNAKADEIDRLLE 362

Query: 325 HLNLLRKMGATIVDNLQISNVDVI--------LNSYESGEFIAIIAEFKVALNDYLKKLI 384
            +  + + GA + +N    N+DVI        L S    E+  +  +F   +  YL ++ 
Sbjct: 363 VVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVK 422

Query: 385 RSPVKSLADIISFNNNH-------PELEKMKEYGQDAFLLSEQTNGI-GVMEKEAISKMA 444
            + + SL DI+ +NN +       P +      GQD FL S +  G+      +A+  + 
Sbjct: 423 NTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVR 482

Query: 445 NLSRN-GFEELMK---GNNLDAMVTIGIGVESVLAI-------GGYPGISVPAGYEENGE 504
             S++ G +  +        D+ +  G+ V S  +I        GYP I++P G + NG 
Sbjct: 483 RTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIGVKTNGR 542

Query: 505 PFGILFGGLKGSEPKLIEIAYAYEQATMVREPP 509
           PFG+        EP+LI+   A E     +  P
Sbjct: 543 PFGLGIMHSAWQEPQLIKYGSAIEDLLQYKAKP 570

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BLAST of Chy1G006930 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 4.2e-63
Identity = 172/508 (33.86%), Postives = 261/508 (51.38%), Query Frame = 0


Query: 19  GVANSSHFSIDEATI-AEIQNAFSQNKLTSTQLLDYYLKKIHLLNPVLKSVLELNPDARA 78
           G+      S+D   +   +Q  + Q  +    ++  Y+ +I  +N  +++V E+NPDA  
Sbjct: 76  GLTKGCFTSVDLVKVRITLQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALT 135

Query: 79  QAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGSFALLGSVVPRDATVVSR 138
            A+  D ER +  GK  G LHG+PI++K+ I T D +++TAGS+A+ G+    DATV ++
Sbjct: 136 IAKQMDNERKM--GKLRGPLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATK 195

Query: 139 LRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKGGDPCGSSSGSAISVAAN 198
           LR AG VI+GK+  ++W   RS    NGW A GGQ    Y K  DP GSSSGS ++    
Sbjct: 196 LREAGLVIMGKSGASQWANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLG 255

Query: 199 MVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVY 258
           +   +LGTET GSI+ PAD +++VG+KPTVGLTSR  V+P++ RQDT+GP+ R+V DA Y
Sbjct: 256 LAFATLGTETSGSIVSPADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAY 315

Query: 259 VLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLGIVRHPFSDLYPNNSMAI 318
           +L+ I G D  D   +      IP   Y +    + LKGKR+G+ R+    ++ +    +
Sbjct: 316 LLQVIAGKDSNDNYTSAIPFDTIP--DYVKACDINALKGKRIGVPRNVIK-IFGSPQTVV 375

Query: 319 PTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIAEFKVALNDYLKKLIRSP 378
             F Q L +++K GA IV+N   ++      S    +   + A+    L  + K+L  +P
Sbjct: 376 DQFNQALAVMKKAGAIIVENTDFTSFAEFAQSPIPDDI--LYADSLTNLPAFFKQLKVNP 435

Query: 379 --VKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKEAISKMANL---SRNG 438
             +  L  +  F  +H  LE+        + ++ Q        K       N+   +  G
Sbjct: 436 HNITDLESLRRFTQHH-RLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGG 495

Query: 439 FEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGE---------------PF 498
               ++ + LDA V        + A+ G P I+VP G   NG                P 
Sbjct: 496 ILGALRRHKLDAAVLPTDLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPGIPI 555

Query: 499 GILFGGLKGSEPKLIEIAYAYEQATMVR 506
           GI F G   SE KLI +AYA+EQ T  R
Sbjct: 556 GIGFMGDLWSEEKLIGLAYAFEQKTHAR 575

[Close]

BLAST of Chy1G006930 vs. ExPASy Swiss-Prot
Match: B0JSX3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=gatA PE=3 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 2.1e-46
Identity = 162/502 (32.27%), Postives = 246/502 (49.00%), Query Frame = 0


Query: 32  TIAEIQNAFSQNKLTSTQLLDYYLKKIHLLNPVLKSVLELNPD-ARAQAEAADRERLLAG 91
           +I ++       + T+ ++   +L +I  L P +KS L L PD A AQA+  D +  +A 
Sbjct: 3   SIRQLHQQLVNKEKTAVEIATEFLARIQALEPQVKSFLHLTPDLALAQAQKVDEK--IAR 62

Query: 92  GKALGELHGIPILLKDAIATKDLLNTTAGSFALLGSVVPRDATVVSRLRNAGAVILGKTS 151
           G++L  L GIPI LKD + TK  + TT  S  L   V P ++TV  +LR+ GAVI+GKT+
Sbjct: 63  GESLHLLAGIPIALKDNLCTKG-IPTTCASRILENFVPPYESTVTQKLRDLGAVIVGKTN 122

Query: 152 LTEWYKSRSYEIPNGWCARGGQAVNPYGKGGDPCGSSSGSAISVAANMVAVSLGTETDGS 211
           L E+    S E  +G+        NP+     P GSS GSA +VAA    V+LG++T GS
Sbjct: 123 LDEFAMGSSTE-NSGYHV----TANPWDLSRVPGGSSGGSAAAVAAQECVVALGSDTGGS 182

Query: 212 ILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYVLEAIVGFDPMDY 271
           I  PA +  VVG+KPT GL SR G++      D IGP  RTV DA  +L+AI G+DP D 
Sbjct: 183 IRQPASFCGVVGLKPTYGLVSRFGLVAYASSLDQIGPFGRTVEDAAILLQAIAGYDPQD- 242

Query: 272 EVTKEASQFIPPGGYKQFLRKDGLKGKRLGIVRHPFSDLYPNNSMAIPTFEQHLNLLRKM 331
                 S  +P   Y QFL K  LKG ++G+++  F +    + +      Q L  L+ +
Sbjct: 243 ----STSLNLPIPDYSQFL-KTSLKGLKIGVIKETFGE--GLDQVVAEAVNQALAQLKAL 302

Query: 332 GATIVDNLQISNVDVILNSYESGEFIAIIAEFKVALN----DYLKKLIRSPVKSLADIIS 391
           GATI + +        L +Y       IIA  + + N    D +K  IR    SL D+ +
Sbjct: 303 GATIKE-ISCPRFRYGLPTY------YIIAPSEASANLARYDGVKYGIREDADSLIDMYT 362

Query: 392 FNNNHPELEKMKEYGQDA---FLLSEQTNGIGVMEKEAI--SKMANLSRNGFEELMKGNN 451
                    + K +G +     +L   T   G  +   +   K+  L +  F+   +  +
Sbjct: 363 -------KTRAKGFGAEVKRRIMLGTYTLSAGYYDAYYLKAQKVRTLIKEDFDRAFQ--S 422

Query: 452 LDAMVT-----------------IGIGVESVLAI----GGYPGISVPAGYEENGEPFGIL 503
           +D +V+                 + + +  ++ I     G PG+S+P G++  G P G+ 
Sbjct: 423 VDVLVSPTSPTTAFKAGEKTADPLSMYLSDLMTIPVNLAGLPGLSLPCGFDGQGLPIGLQ 472

[Close]

BLAST of Chy1G006930 vs. ExPASy Swiss-Prot
Match: Q6MRL7 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIMB 9529 / HD100) OX=264462 GN=gatA PE=3 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 5.4e-42
Identity = 151/509 (29.67%), Postives = 249/509 (48.92%), Query Frame = 0


Query: 31  ATIAEIQNAFSQNKLTSTQLLDYYLKKIHLLNPVLKSVLELNPDARAQAEAADRERLLAG 90
           A+++EI  A +   +++ ++  ++LK+I  LNP L +   LNP A  +AEA D    +A 
Sbjct: 6   ASLSEISEAVNNRSISAKEVTLHFLKRIENLNPKLNAFTSLNPQAVQEAEAVDAR--IAN 65

Query: 91  GKALGELHGIPILLKDAIATKDLLNTTAGSFALLGSVVPRDATVVSRLRNAGAVILGKTS 150
           G+ +G L G+P  +K+   TK  L TTAGS  L   V P DAT V+RL+ +G V++GK +
Sbjct: 66  GEDVGLLAGVPFGIKEMFCTKG-LTTTAGSKILENFVPPYDATAVARLKKSGIVVMGKLN 125

Query: 151 LTEWYKSRSYEIPNGWCARGGQAVNPYGKGGDPCGSSSGSAISVAANMVAVSLGTETDGS 210
             E+    S E      +  G   NP+     P GSS GSA + A+ +VA +LGT+T GS
Sbjct: 126 QDEFAMGSSNE-----TSFHGVVKNPWDLERVPGGSSGGSAAAQASRLVAGTLGTDTGGS 185

Query: 211 ILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYVLEAIVGFDPMDY 270
           I  PA +  +VG+KPT G  SR G++      D  GP+  +V DA   LE I GFDP D 
Sbjct: 186 IRQPASFCGIVGVKPTYGRVSRYGIVAYASSLDQAGPMVSSVRDAALTLEVISGFDPQDS 245

Query: 271 EVTKEASQFIPPGGYKQFLRKDGLKGKRLGIVRHPFSDLYPNNSMAIPTFEQHLNLLRKM 330
             T++    +P   + Q L+ D +KG ++G+++   +     +     T E  ++ L+++
Sbjct: 246 TTTQKQ---VP--AWSQNLKAD-VKGMKIGLMKEYMTGALDPDVQ--KTVENSVDTLKQL 305

Query: 331 GATIVDNLQISNVDVILNSYESGEFIAII---AEFKVALNDYLKKLIRSPVKSLA--DII 390
           GA IV+      V V + ++    +  +    A   ++  D +K   R+  K+L+  D+ 
Sbjct: 306 GAEIVE------VSVPMTAFAVPVYYLVAASEASSNLSRYDGVKYGYRAEFKNLSAVDLE 365

Query: 391 SFNNNHPELEKMKEYGQDA---FLLSEQTNGIGVMEKEAISKMANLSRNGFEELMKG-NN 450
            F +      + + +G +     +L       G  +    +K   + R   E+ ++    
Sbjct: 366 EFYSQ----TRGQAFGAEVKRRIMLGTYCLSSGYYD-AFYNKAGQVRRLIMEQYLEAFKK 425

Query: 451 LDAMVT---------IGIGVESVLA------------IGGYPGISVPAGYEENGEPFGIL 510
            D +++         IG  V   LA            + G PG+SVP G  ++G P GI 
Sbjct: 426 CDVILSPVTTAPAFKIGERVSDPLAMYLNDIFTTSTNLAGLPGMSVPFGQSQSGLPIGIQ 485

[Close]

BLAST of Chy1G006930 vs. ExPASy TrEMBL
Match: A0A0A0K188 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009770 PE=4 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.4e-279
Identity = 503/517 (97.29%), Postives = 510/517 (98.65%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNIV    FFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL
Sbjct: 1   MNIV----FFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVLKSVLELNPDARAQAEAADRERLLAGGKA GELHG+PILLKDAIATKDLLNTTAGS
Sbjct: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKARGELHGVPILLKDAIATKDLLNTTAGS 120

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRS+EIPNGWCARGGQAVNPYG+G
Sbjct: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSFEIPNGWCARGGQAVNPYGRG 180

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP
Sbjct: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIP GGYKQFLRK+GLKGKRLG
Sbjct: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRKNGLKGKRLG 300

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPFSDLYPNNS+AIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA
Sbjct: 301 IVRHPFSDLYPNNSIAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFKVA+NDYLKKLIRSPV+SLADIISFNNNH ELEKMKEYGQDAFLLSEQTNGIGVMEKE
Sbjct: 361 EFKVAINDYLKKLIRSPVRSLADIISFNNNHAELEKMKEYGQDAFLLSEQTNGIGVMEKE 420

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL
Sbjct: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 518
           FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL
Sbjct: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 513

[Close]

BLAST of Chy1G006930 vs. ExPASy TrEMBL
Match: A0A1S3BIN0 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103490302 PE=4 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 2.5e-268
Identity = 486/517 (94.00%), Postives = 500/517 (96.71%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNIV   FFFLSAVLLFTG+ANSS+FSIDEATI EIQNAFSQNKLTSTQLLDYYL KIHL
Sbjct: 33  MNIV---FFFLSAVLLFTGLANSSYFSIDEATIVEIQNAFSQNKLTSTQLLDYYLNKIHL 92

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVLKSVLELNPDARAQAEAADRERLLA GK+LGELHGIPILLKDAIATKDLLNTTAGS
Sbjct: 93  LNPVLKSVLELNPDARAQAEAADRERLLACGKSLGELHGIPILLKDAIATKDLLNTTAGS 152

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEW+KSRS +IPNGWCARGGQAVNPYGKG
Sbjct: 153 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWFKSRSSKIPNGWCARGGQAVNPYGKG 212

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++P
Sbjct: 213 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISP 272

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIP GGYKQFLRKDGLKGKRLG
Sbjct: 273 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRKDGLKGKRLG 332

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPFSDLY NNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILN YESGEFIAIIA
Sbjct: 333 IVRHPFSDLYSNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNPYESGEFIAIIA 392

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFK+A+NDYLKKLI+SPV+SLADIISFNN HPELE MKEYGQDAFLLSEQTNGIG MEKE
Sbjct: 393 EFKLAVNDYLKKLIQSPVRSLADIISFNNKHPELENMKEYGQDAFLLSEQTNGIGEMEKE 452

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AIS+M NLSRNGFEELMKGNNLDAMVTIG G+ESVLAIGGYPGISVPAGYEENGEPFGIL
Sbjct: 453 AISRMMNLSRNGFEELMKGNNLDAMVTIGTGIESVLAIGGYPGISVPAGYEENGEPFGIL 512

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 518
           FGGLKGSEPKLIEIAYAYEQATMVRE PPLLS+I SL
Sbjct: 513 FGGLKGSEPKLIEIAYAYEQATMVRE-PPLLSRISSL 545

[Close]

BLAST of Chy1G006930 vs. ExPASy TrEMBL
Match: A0A5A7U802 (Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00790 PE=4 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 8.1e-267
Identity = 484/517 (93.62%), Postives = 499/517 (96.52%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNIV   FFFLSAVLLFTG+ANSS+FSIDEATI EIQNAFSQNKLTSTQLLDYYL KIHL
Sbjct: 1   MNIV---FFFLSAVLLFTGLANSSYFSIDEATIVEIQNAFSQNKLTSTQLLDYYLNKIHL 60

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVLKSVLELNPDARAQAEAADRERLLA GK+LGELHGIPILLKDAIATKDLLNTTAGS
Sbjct: 61  LNPVLKSVLELNPDARAQAEAADRERLLACGKSLGELHGIPILLKDAIATKDLLNTTAGS 120

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEW+KSRS +IPNGWCARGGQAVNPYGKG
Sbjct: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWFKSRSSKIPNGWCARGGQAVNPYGKG 180

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++P
Sbjct: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISP 240

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIP GGYKQFLR DGLKGKRLG
Sbjct: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRIDGLKGKRLG 300

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPFSDLY NNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILN YESGEFIAIIA
Sbjct: 301 IVRHPFSDLYSNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNPYESGEFIAIIA 360

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFK+A+NDYLKKLI+SPV+SLADIISFNN HPELE MKEYGQDAFLLSEQTNGIG MEKE
Sbjct: 361 EFKLAVNDYLKKLIQSPVRSLADIISFNNKHPELENMKEYGQDAFLLSEQTNGIGEMEKE 420

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AIS+M NLSRNGFEELMKGN+LDAMVTIG G+ESVLAIGGYPGISVPAGYEENGEPFGIL
Sbjct: 421 AISRMMNLSRNGFEELMKGNHLDAMVTIGTGIESVLAIGGYPGISVPAGYEENGEPFGIL 480

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 518
           FGGLKGSEPKLIEIAYAYEQATMVRE PPLLS+I SL
Sbjct: 481 FGGLKGSEPKLIEIAYAYEQATMVRE-PPLLSRISSL 513

[Close]

BLAST of Chy1G006930 vs. ExPASy TrEMBL
Match: A0A6J1ENQ3 (putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111435192 PE=4 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 8.7e-237
Identity = 429/517 (82.98%), Postives = 460/517 (88.97%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNIV    FFLS  LLF G ANSS F IDEATIAEIQ+AFSQNKLTS +LLD+YL KI  
Sbjct: 36  MNIV----FFLSVFLLFVG-ANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDF 95

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVL+SVLE+NPDARAQAE ADRER LAGGKALGELHGIP+LLKD+I TKD LNTTAGS
Sbjct: 96  LNPVLRSVLEVNPDARAQAETADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGS 155

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDATVV RLRNAGAVILGKTSLTEWY SRS +IP+GWCARGGQA+NPYGKG
Sbjct: 156 FALLGSVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLKIPHGWCARGGQALNPYGKG 215

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANM AVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++P
Sbjct: 216 GDPCGSSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISP 275

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVL+AIVGFDPMD E TK  SQFIP GGYKQFL++DGL GKRLG
Sbjct: 276 RQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLG 335

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPFSDLY N S AI TFE H+ LLRK GATIVDNLQISNV  ILN YESGE +A+IA
Sbjct: 336 IVRHPFSDLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIA 395

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFK+ +NDYLK LI+SPV+SLADII+FNNNHPELE MKEYGQDAFLLSEQT GIG  EK 
Sbjct: 396 EFKLTINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKA 455

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AIS M+NLSR+GFEE+MK  NLDAMVT+G G E+VLAIGGYP ISVPAGYE NGEPFG+L
Sbjct: 456 AISMMSNLSRDGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVL 515

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 518
           F GLKG+EPKLIEIAYAYEQATMVR PPPLLS + SL
Sbjct: 516 FSGLKGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547

[Close]

BLAST of Chy1G006930 vs. ExPASy TrEMBL
Match: A0A6J1JLA2 (putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111486234 PE=4 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 4.3e-236
Identity = 429/517 (82.98%), Postives = 459/517 (88.78%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNIV    FFLSA L F G ANSS F IDEAT+AEIQ+AFSQNKLTS +LLD+YL KI  
Sbjct: 1   MNIV----FFLSAFLFFIG-ANSSQFPIDEATVAEIQHAFSQNKLTSRELLDHYLNKIDF 60

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVLKSVLE+NPDARAQAEAADRER LAGGKALGELHGIP+LLKD+I TKD LNTTAGS
Sbjct: 61  LNPVLKSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGS 120

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDA VV RLRNAGAVILGKTSLTEWY SRS +IP+GWCARGGQA+NPYGKG
Sbjct: 121 FALLGSVVPRDAKVVHRLRNAGAVILGKTSLTEWYGSRSMKIPHGWCARGGQALNPYGKG 180

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANM AVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++P
Sbjct: 181 GDPCGSSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISP 240

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVL+AIVGFDPMD E TK  SQFIP GGYKQFL++DGL GKRLG
Sbjct: 241 RQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLG 300

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPFSDLY N S AI +FE H+ LLRK GATIVDNLQISNV  ILN YESGE +A+IA
Sbjct: 301 IVRHPFSDLYANESTAIQSFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIA 360

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFK+ +NDYLK LI+SPV+SLADII+FNNNHPELE MKEYGQDAFLLSEQT GIG  EK 
Sbjct: 361 EFKLTINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKA 420

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AIS M NLSR+GFEE+MK  NLDAMVT+G G E+VLAIGGYP ISVPAGYE NGEPFGIL
Sbjct: 421 AISMMENLSRHGFEEMMKTYNLDAMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGIL 480

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 518
           F GLKG+EPKLIEIAYAYEQATMVR PPPLLS + SL
Sbjct: 481 FSGLKGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 512

[Close]

BLAST of Chy1G006930 vs. NCBI nr
Match: XP_004144836.1 (probable amidase At4g34880 [Cucumis sativus] >KGN43233.1 hypothetical protein Csa_020185 [Cucumis sativus])

HSP 1 Score: 969 bits (2506), Expect = 0.0
Identity = 503/517 (97.29%), Postives = 510/517 (98.65%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNIVFF    LSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL
Sbjct: 1   MNIVFF----LSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVLKSVLELNPDARAQAEAADRERLLAGGKA GELHG+PILLKDAIATKDLLNTTAGS
Sbjct: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKARGELHGVPILLKDAIATKDLLNTTAGS 120

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRS+EIPNGWCARGGQAVNPYG+G
Sbjct: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSFEIPNGWCARGGQAVNPYGRG 180

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP
Sbjct: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIP GGYKQFLRK+GLKGKRLG
Sbjct: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRKNGLKGKRLG 300

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPFSDLYPNNS+AIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA
Sbjct: 301 IVRHPFSDLYPNNSIAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFKVA+NDYLKKLIRSPV+SLADIISFNNNH ELEKMKEYGQDAFLLSEQTNGIGVMEKE
Sbjct: 361 EFKVAINDYLKKLIRSPVRSLADIISFNNNHAELEKMKEYGQDAFLLSEQTNGIGVMEKE 420

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL
Sbjct: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 517
           FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL
Sbjct: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 513

[Close]

BLAST of Chy1G006930 vs. NCBI nr
Match: XP_008447974.1 (PREDICTED: putative amidase C869.01 [Cucumis melo])

HSP 1 Score: 932 bits (2409), Expect = 0.0
Identity = 486/517 (94.00%), Postives = 500/517 (96.71%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNIVFFF   LSAVLLFTG+ANSS+FSIDEATI EIQNAFSQNKLTSTQLLDYYL KIHL
Sbjct: 33  MNIVFFF---LSAVLLFTGLANSSYFSIDEATIVEIQNAFSQNKLTSTQLLDYYLNKIHL 92

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVLKSVLELNPDARAQAEAADRERLLA GK+LGELHGIPILLKDAIATKDLLNTTAGS
Sbjct: 93  LNPVLKSVLELNPDARAQAEAADRERLLACGKSLGELHGIPILLKDAIATKDLLNTTAGS 152

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEW+KSRS +IPNGWCARGGQAVNPYGKG
Sbjct: 153 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWFKSRSSKIPNGWCARGGQAVNPYGKG 212

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++P
Sbjct: 213 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISP 272

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIP GGYKQFLRKDGLKGKRLG
Sbjct: 273 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRKDGLKGKRLG 332

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPFSDLY NNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILN YESGEFIAIIA
Sbjct: 333 IVRHPFSDLYSNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNPYESGEFIAIIA 392

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFK+A+NDYLKKLI+SPV+SLADIISFNN HPELE MKEYGQDAFLLSEQTNGIG MEKE
Sbjct: 393 EFKLAVNDYLKKLIQSPVRSLADIISFNNKHPELENMKEYGQDAFLLSEQTNGIGEMEKE 452

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AIS+M NLSRNGFEELMKGNNLDAMVTIG G+ESVLAIGGYPGISVPAGYEENGEPFGIL
Sbjct: 453 AISRMMNLSRNGFEELMKGNNLDAMVTIGTGIESVLAIGGYPGISVPAGYEENGEPFGIL 512

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 517
           FGGLKGSEPKLIEIAYAYEQATMVREPP LLS+I SL
Sbjct: 513 FGGLKGSEPKLIEIAYAYEQATMVREPP-LLSRISSL 545

[Close]

BLAST of Chy1G006930 vs. NCBI nr
Match: KAA0049735.1 (putative amidase [Cucumis melo var. makuwa] >TYK12142.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 927 bits (2396), Expect = 0.0
Identity = 484/517 (93.62%), Postives = 499/517 (96.52%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNIVFFF   LSAVLLFTG+ANSS+FSIDEATI EIQNAFSQNKLTSTQLLDYYL KIHL
Sbjct: 1   MNIVFFF---LSAVLLFTGLANSSYFSIDEATIVEIQNAFSQNKLTSTQLLDYYLNKIHL 60

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVLKSVLELNPDARAQAEAADRERLLA GK+LGELHGIPILLKDAIATKDLLNTTAGS
Sbjct: 61  LNPVLKSVLELNPDARAQAEAADRERLLACGKSLGELHGIPILLKDAIATKDLLNTTAGS 120

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEW+KSRS +IPNGWCARGGQAVNPYGKG
Sbjct: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWFKSRSSKIPNGWCARGGQAVNPYGKG 180

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++P
Sbjct: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISP 240

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIP GGYKQFLR DGLKGKRLG
Sbjct: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPSGGYKQFLRIDGLKGKRLG 300

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPFSDLY NNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILN YESGEFIAIIA
Sbjct: 301 IVRHPFSDLYSNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNPYESGEFIAIIA 360

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFK+A+NDYLKKLI+SPV+SLADIISFNN HPELE MKEYGQDAFLLSEQTNGIG MEKE
Sbjct: 361 EFKLAVNDYLKKLIQSPVRSLADIISFNNKHPELENMKEYGQDAFLLSEQTNGIGEMEKE 420

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AIS+M NLSRNGFEELMKGN+LDAMVTIG G+ESVLAIGGYPGISVPAGYEENGEPFGIL
Sbjct: 421 AISRMMNLSRNGFEELMKGNHLDAMVTIGTGIESVLAIGGYPGISVPAGYEENGEPFGIL 480

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 517
           FGGLKGSEPKLIEIAYAYEQATMVREPP LLS+I SL
Sbjct: 481 FGGLKGSEPKLIEIAYAYEQATMVREPP-LLSRISSL 513

[Close]

BLAST of Chy1G006930 vs. NCBI nr
Match: XP_038887903.1 (probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 881 bits (2277), Expect = 0.0
Identity = 456/517 (88.20%), Postives = 480/517 (92.84%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNIVFF    LSA LLFTG A+SS F IDEATIAEIQNAFSQNKLT+TQLL++YL KIH 
Sbjct: 45  MNIVFF----LSAFLLFTGDASSSDFPIDEATIAEIQNAFSQNKLTTTQLLEHYLNKIHF 104

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVLKSVLE+NPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKD LNTTAGS
Sbjct: 105 LNPVLKSVLEVNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDRLNTTAGS 164

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWY SRS +IPNGWCARGGQAVNPYGKG
Sbjct: 165 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYGSRSLKIPNGWCARGGQAVNPYGKG 224

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANMV VSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++P
Sbjct: 225 GDPCGSSSGSAISVAANMVTVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISP 284

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVLEAIVGFDPMD E TKEASQFIP GGYKQFLRKDGLKGKRLG
Sbjct: 285 RQDTIGPICRTVSDAVYVLEAIVGFDPMDCEATKEASQFIPSGGYKQFLRKDGLKGKRLG 344

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPF DLYPN+SMAIPTFEQHLNLLRK GATIVDNLQI+NVDVILN + SGEFIA IA
Sbjct: 345 IVRHPFFDLYPNDSMAIPTFEQHLNLLRKSGATIVDNLQITNVDVILNPFGSGEFIATIA 404

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFK+ +NDYLKKLI+SPV+SLADII+FNNNHPELE MKEYGQDAFLLSEQT+GIG  EKE
Sbjct: 405 EFKLGINDYLKKLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTDGIGETEKE 464

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AIS+M NLS  GFEE+MKGN+LDAM+T+G G E VLAIGGYP ISVPAGYE NGEPFGIL
Sbjct: 465 AISRMMNLSLYGFEEIMKGNSLDAMITLGTGAEGVLAIGGYPAISVPAGYEGNGEPFGIL 524

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 517
            GGLKG+EPKLIEIAYAYEQATMVR PPPLLSQ+ SL
Sbjct: 525 LGGLKGTEPKLIEIAYAYEQATMVRRPPPLLSQLSSL 557

[Close]

BLAST of Chy1G006930 vs. NCBI nr
Match: KAG7015266.1 (hypothetical protein SDJN02_22900 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 827 bits (2137), Expect = 1.72e-298
Identity = 428/517 (82.79%), Postives = 461/517 (89.17%), Query Frame = 0


Query: 1   MNIVFFFFFFLSAVLLFTGVANSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHL 60
           MNI+FF    LS  LLF G ANSS F IDEATIAEIQ+AFSQNKLTS +LLD+YL KI  
Sbjct: 36  MNIIFF----LSVFLLFVG-ANSSQFPIDEATIAEIQHAFSQNKLTSRELLDHYLNKIDF 95

Query: 61  LNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGS 120
           LNPVL+SVLE+NPDARAQAEAADRER LAGGKALGELHGIP+LLKD+I TKD LNTTAGS
Sbjct: 96  LNPVLRSVLEVNPDARAQAEAADRERELAGGKALGELHGIPVLLKDSIGTKDRLNTTAGS 155

Query: 121 FALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKG 180
           FALLGSVVPRDATVV RLRNAGAVILGKTSLTEWY SRS +IP+GWCARGGQA+NPYGKG
Sbjct: 156 FALLGSVVPRDATVVHRLRNAGAVILGKTSLTEWYGSRSLQIPHGWCARGGQALNPYGKG 215

Query: 181 GDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTP 240
           GDPCGSSSGSAISVAANM AVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIP++P
Sbjct: 216 GDPCGSSSGSAISVAANMAAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPISP 275

Query: 241 RQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDGLKGKRLG 300
           RQDTIGPICRTVSDAVYVL+AIVGFDPMD E TK  SQFIP GGYKQFL++DGL GKRLG
Sbjct: 276 RQDTIGPICRTVSDAVYVLDAIVGFDPMDSEATKVGSQFIPSGGYKQFLKRDGLTGKRLG 335

Query: 301 IVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIA 360
           IVRHPFSDLY N S AI TFE H+ LLRK GATIVDNLQISNV  ILN YESGE +A+IA
Sbjct: 336 IVRHPFSDLYANGSKAIQTFEHHVKLLRKSGATIVDNLQISNVAAILNPYESGELVAMIA 395

Query: 361 EFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNGIGVMEKE 420
           EFK+ +NDYLK LI+SPV+SLADII+FNNNHPELE MKEYGQDAFLLSEQT GIG  EK 
Sbjct: 396 EFKLTINDYLKNLIQSPVRSLADIIAFNNNHPELENMKEYGQDAFLLSEQTEGIGEAEKA 455

Query: 421 AISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEENGEPFGIL 480
           AIS M+NLSR+GFEE+MK  NLD+MVT+G G E+VLAIGGYP ISVPAGYE NGEPFG+L
Sbjct: 456 AISMMSNLSRDGFEEMMKTYNLDSMVTLGTGAETVLAIGGYPAISVPAGYEGNGEPFGVL 515

Query: 481 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILSL 517
           F GLKG+EPKLIEIAYAYEQATMVR PPPLLS + SL
Sbjct: 516 FSGLKGTEPKLIEIAYAYEQATMVRRPPPLLSDLSSL 547

[Close]

BLAST of Chy1G006930 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 496.5 bits (1277), Expect = 2.6e-140
Identity = 277/519 (53.37%), Postives = 356/519 (68.59%), Query Frame = 0


Query: 5   FFFFFFL-------SAVLLFTGVAN----SSHFSIDEATIAEIQNAFSQNKLTSTQLLDY 64
           FFFF  L        A+++  G A+    SS FSI EATI +I+ AF++ +LTS QL++ 
Sbjct: 6   FFFFSSLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVEL 65

Query: 65  YLKKIHLLNPVLKSVLELNPDARAQAEAADRERLLAGGKALGELHGIPILLKDAIATKDL 124
           YL+ I  LNP+L +V+E NPDA  QAE ADRER L     L  LHG+P+LLKD+I+TKD 
Sbjct: 66  YLEAISKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDK 125

Query: 125 LNTTAGSFALLGSVVPRDATVVSRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQA 184
           LNTTAGSFALLGSVV RDA VV RLR +GAVILGK SL+EW   RS+ IP+GW A     
Sbjct: 126 LNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA----- 185

Query: 185 VNPYGKGGDPCGSSSGSAISVAANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRA 244
                                                       NSVVGIKP+VGLTSRA
Sbjct: 186 -----------------------------------------PSQNSVVGIKPSVGLTSRA 245

Query: 245 GVIPVTPRQDTIGPICRTVSDAVYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDG 304
           GV+P++ RQD+IGPICRTVSDAV++L+AIVG+DP+D E TK AS+FIP GGYKQFL   G
Sbjct: 246 GVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLD-EATKTASEFIPEGGYKQFLTTSG 305

Query: 305 LKGKRLGIVRHPFSDLYPNNSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESG 364
           LKGKRLGIV    S L           + H+  LR+ GA +++NL I N++VI+   +SG
Sbjct: 306 LKGKRLGIVMKHSSLL-----------DHHIKTLRREGAIVINNLTIPNIEVIVGGTDSG 365

Query: 365 EFIAIIAEFKVALNDYLKKLIRSPVKSLADIISFNNNHPELEKMKEYGQDAFLLSEQTNG 424
           E IA++AEFK++LN YLK+L++SPV+SLAD+I++N    E EK+KE+GQ+ FL +E T+G
Sbjct: 366 EEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSG 425

Query: 425 IGVMEKEAISKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIGGYPGISVPAGYEEN 484
           +G  EK A+ KM  LSRNG E+L++ N LDA+VT+G  + SVLAIGGYPGI+VPAGY+  
Sbjct: 426 MGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSG 466

Query: 485 GEPFGILFGGLKGSEPKLIEIAYAYEQATMVREPPPLLS 513
           G P+GI FGGL+ SEPKLIEIA+A+EQAT++R+PP  ++
Sbjct: 486 GVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPPKFIA 466

[Close]

BLAST of Chy1G006930 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 130.6 bits (327), Expect = 3.7e-30
Identity = 144/534 (26.97%), Postives = 232/534 (43.45%), Query Frame = 0


Query: 22  NSSHFSIDEATIAEIQNAFSQNKLTSTQLLDYYLKKIHLLNPVLKSVLELNPDARAQAEA 81
           ++S  S  ++ I   + +    + T+ ++   YL +I L  P LK  L ++ +    A+ 
Sbjct: 35  DTSVISPPQSQILTTRRSLLSGETTAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQE 94

Query: 82  ADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGSFALLGSVVPRDATVVSRLRNA 141
            D+   +A G+ LG L G+ I +KD I T+  + +TA S  L     P DAT V +++  
Sbjct: 95  IDQR--IAKGEELGPLAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFDATAVKKIKEL 154

Query: 142 GAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKGGDPCGSSSGSAISVAANMVAV 201
           G +++GKT++ E+    + E      +      NP+     P GSS GSA +VAA    V
Sbjct: 155 GGIVVGKTNMDEFGMGSTTE-----ASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMV 214

Query: 202 SLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYVLEA 261
           SLG++T GS+  PA +  VVG+KPT G  SR G++      D IG    TV+DA  +L A
Sbjct: 215 SLGSDTGGSVRQPASFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHA 274

Query: 262 IVGFDPMDYEVTKEASQFIPPGGYKQFLRKD-----GLKGKRLGIVRHPFSDLYPNNSMA 321
           I G+D  D   +K+      P    QFL  D      L G ++GI+R    D        
Sbjct: 275 ISGYDRFDSTSSKQD----VPEFQSQFLSVDHFESKPLNGVKVGIIRETLED-------- 334

Query: 322 IPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIAEFKVALNDYLKKLIRS 381
               +  +    +  A+ ++ L     +V L S+  G    + A + +A ++    L R 
Sbjct: 335 --GVDSGVRSATQEAASHLEALGCILTEVSLPSFSLG----LPAYYVIASSESSSNLSR- 394

Query: 382 PVKSLADIISFNNN--HPELEKMKEY-------GQDAFLLSEQTNGIGVMEKEAISKMA- 441
                 D + + N     EL K+ E        G+    +   T  +     +A  K A 
Sbjct: 395 -----YDGVRYGNQVMAEELNKLYECSRGEGFGGEVKMRILMGTYALSAGYYDAYYKRAQ 454

Query: 442 ---NLSRNGFEELMKGNNL-------DAMVTIGIGVESVLA------------IGGYPGI 501
               L R  F+  ++ N++        A   IG   +  LA            + G P +
Sbjct: 455 QVRTLIRKDFKAALEQNDILISPAAPSAAYKIGEKKDDPLAMYAGDIMTVNVNLAGLPAM 514

Query: 502 SVPAGYEENGE---PFGILFGGLKGSEPKLIEIAYAYEQATMVRE-PPPLLSQI 515
            +P G  E G    P G+   G    E KL+++ + +EQ        PPLL+ +
Sbjct: 515 VLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLKGSSFVPPLLANV 536

[Close]

BLAST of Chy1G006930 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 118.6 bits (296), Expect = 1.5e-26
Identity = 81/238 (34.03%), Postives = 126/238 (52.94%), Query Frame = 0


Query: 32  TIAEIQNAFSQNKLTSTQLLDYYLKKIHLLNPVLKSVLELNPDARAQAEAADRERLLAGG 91
           ++ E+       ++TS +L+  YLK++   N VL++V+    +  A  +A + + LL+ G
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEEL-AYKQAKEADDLLSQG 263

Query: 92  KALGELHGIPILLKDAIATKDLLNTTAGSFALLGSVVPRDATVVSRLRNAGAVILGKTSL 151
             LG LHGIP  LKD +A      TT GS +     +  +A V  RL+ +GAV++ K   
Sbjct: 264 TYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAK--- 323

Query: 152 TEWYKSRSYEIPNGWCARGGQAVNPYGKGGDPCGSSSGSAISVAANMVAVSLGTETDGSI 211
                + S    + W   GG+  NP+       GSS+G A S +A MV  ++G+ET GS+
Sbjct: 324 ---LVTGSMAYDDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSM 383

Query: 212 LCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYVLEAIVGFDPMD 270
             PA    +  ++PT G   R GV+ ++   D +GP CRT +D   +L+AI G DP D
Sbjct: 384 TYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 431

[Close]

BLAST of Chy1G006930 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 108.2 bits (269), Expect = 2.0e-23
Identity = 79/238 (33.19%), Postives = 122/238 (51.26%), Query Frame = 0


Query: 32  TIAEIQNAFSQNKLTSTQLLDYYLKKIHLLNPVLKSVLELNPDARAQAEAADRERLLAGG 91
           ++ E+       ++TS +L+  YLK++   N VL++V+    +  A  +A + + LL+ G
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEEL-AYKQAKEADDLLSQG 263

Query: 92  KALGELHGIPILLKDAIATKDLLNTTAGSFALLGSVVPRDATVVSRLRNAGAVILGKTSL 151
             LG LHGIP  LKD +A      TT GS +     +  +A V  RL+ +GAV++ K   
Sbjct: 264 TYLGPLHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAK--- 323

Query: 152 TEWYKSRSYEIPNGWCARGGQAVNPYGKGGDPCGSSSGSAISVAANMVAVSLGTETDGSI 211
                + S    + W   GG+  NP+       GSS+G A S +A       G+ET GS+
Sbjct: 324 ---LVTGSMAYDDIWF--GGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSM 383

Query: 212 LCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDAVYVLEAIVGFDPMD 270
             PA    +  ++PT G   R GV+ ++   D +GP CRT +D   +L+AI G DP D
Sbjct: 384 TYPAARCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 424

[Close]

BLAST of Chy1G006930 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 93.6 bits (231), Expect = 5.0e-19
Identity = 122/456 (26.75%), Postives = 193/456 (42.32%), Query Frame = 0


Query: 78  QAEAADRERLLAGGKALGELHGIPILLKDAIATKDLLNTTAGSFALL--GSVVPRDATVV 137
           QAEA+ R      G  +  L GI + +KD I    L + T G    L     V +D+ VV
Sbjct: 180 QAEASTRR--FEQGNPISVLDGIFVTIKDDIDC--LPHPTNGGTTWLHEDRSVEKDSAVV 239

Query: 138 SRLRNAGAVILGKTSLTEWYKSRSYEIPNGWCARGGQAVNPYGKGGDPCGSSSGSAISVA 197
           S+LR+ GA++LGK ++ E     +     G  +  G   NP+       GSSSGSA  VA
Sbjct: 240 SKLRSCGAILLGKANMHELGMGTT-----GNNSNYGTTRNPHDPKRYTGGSSSGSAAIVA 299

Query: 198 ANMVAVSLGTETDGSILCPADYNSVVGIKPTVGLTSRAGVIPVTPRQDTIGPICRTVSDA 257
           A + + +LGT+  GS+  P+    + G+K T G T   G +      + IGP+  ++ DA
Sbjct: 300 AGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLEDA 359

Query: 258 VYVLEAIVGFDPMDYEVTKEASQFIPPGGYKQFLRKDG---LKGKRLGIVRHPFSDLYPN 317
             V  AI+G    D    K +     P  + + L  +G   +   RLG     F+D+  +
Sbjct: 360 FLVYAAILGSSSADRYNLKPS-----PPCFPKLLSHNGSNAIGSLRLGKYTKWFNDV--S 419

Query: 318 NSMAIPTFEQHLNLLRKMGATIVDNLQISNVDVILNSYESGEFIAIIAEFKVALNDYLK- 377
           +S      E  L LL       V  + +      L    +   I+I +    +L  Y + 
Sbjct: 420 SSDISDKCEDILKLLSNNHGCKVVEIVVPE----LEEMRAAHVISIGSPTLSSLTPYCEA 479

Query: 378 ----KLIRSPVKSLADIISFNNN-----HPELEKMKEYGQDAFLLSEQTNGIGVMEKEAI 437
               KL      S A   SF+ +          ++ EY  + F        + V+     
Sbjct: 480 GKNSKLSYDTRTSFAIFRSFSASDYIAAQCLRRRLMEYHLNIF------KDVDVIVTPTT 539

Query: 438 SKMANLSRNGFEELMKGNNLDAMVTIGIGVESVLAIG--GYPGISVPAGYEENGEPFGIL 497
              A +      + +K    +  VT  + +  VLA    G+P ISVP GY++ G P G+ 
Sbjct: 540 GMTAPVIP---PDALKNGETNIQVTTDL-MRFVLAANLLGFPAISVPVGYDKEGLPIGLQ 599

Query: 498 FGGLKGSEPKLIEIAYAYEQATMVREPPPLLSQILS 517
             G   +E  ++ +A A E+   V + P +   IL+
Sbjct: 600 IMGRPWAEATVLGLAAAVEELAPVTKKPAIFYDILN 605

The following BLAST results are available for this feature:

BLAST of Chy1G006930 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1P8B760	2.8e-163	60.12	Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=...	[more]
Q9URY4	5.3e-82	38.60	Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) ...	[more]
D4B3C8	4.2e-63	33.86	Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS ...	[more]
B0JSX3	2.1e-46	32.27	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Microcystis aeruginosa (strain ...	[more]
Q6MRL7	5.4e-42	29.67	Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Bdellovibrio bacteriovorus (str...	[more]

BLAST of Chy1G006930 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K188	1.4e-279	97.29	Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G009770 PE=...	[more]
A0A1S3BIN0	2.5e-268	94.00	putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103490302 PE=4 SV=1	[more]
A0A5A7U802	8.1e-267	93.62	Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G007...	[more]
A0A6J1ENQ3	8.7e-237	82.98	putative amidase C869.01 OS=Cucurbita moschata OX=3662 GN=LOC111435192 PE=4 SV=1	[more]
A0A6J1JLA2	4.3e-236	82.98	putative amidase C869.01 OS=Cucurbita maxima OX=3661 GN=LOC111486234 PE=4 SV=1	[more]

BLAST of Chy1G006930 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144836.1	0.0	97.29	probable amidase At4g34880 [Cucumis sativus] >KGN43233.1 hypothetical protein Cs...	[more]
XP_008447974.1	0.0	94.00	PREDICTED: putative amidase C869.01 [Cucumis melo]	[more]
KAA0049735.1	0.0	93.62	putative amidase [Cucumis melo var. makuwa] >TYK12142.1 putative amidase [Cucumi...	[more]
XP_038887903.1	0.0	88.20	probable amidase At4g34880 [Benincasa hispida]	[more]
KAG7015266.1	1.72e-298	82.79	hypothetical protein SDJN02_22900 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Chy1G006930 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34880.1	2.6e-140	53.37	Amidase family protein	[more]
AT3G25660.1	3.7e-30	26.97	Amidase family protein	[more]
AT5G07360.1	1.5e-26	34.03	Amidase family protein	[more]
AT5G07360.2	2.0e-23	33.19	Amidase family protein	[more]
AT5G64440.1	5.0e-19	26.75	fatty acid amide hydrolase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036928	Amidase signature (AS) superfamily	GENE3D	3.90.1300.10	Amidase signature (AS) domain	coord: 17..510 e-value: 2.7E-145 score: 487.2
IPR036928	Amidase signature (AS) superfamily	SUPERFAMILY	75304	Amidase signature (AS) enzymes	coord: 27..510
IPR023631	Amidase signature domain	PFAM	PF01425	Amidase	coord: 50..398 e-value: 1.5E-72 score: 245.1
None	No IPR available	PANTHER	PTHR42678:SF25	C869.01-LIKE PROTEIN, PUTATIVE-RELATED	coord: 11..513
None	No IPR available	PANTHER	PTHR42678	AMIDASE	coord: 11..513

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy1G006930.1	Chy1G006930.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane