Chy1G006540 (gene) Cucumber (hystrix) v1

Overview
NameChy1G006540
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMechanosensitive ion channel protein
LocationchrH01: 4659404 .. 4663315 (+)
RNA-Seq ExpressionChy1G006540
SyntenyChy1G006540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATACGCTCAAGAAATCTTTCAAAGGCAATGTATCTTTCAAGCATACAAGGAAAATTTCTGCTGGAGGAGCCGGCAGCGAAATCAATCACGAAGAGCTCCCCATTCTTCTCAATCACCAAACTACGGATCATAGTCGTTGTCTAAGGGATCCTCATCCGGTGAACGATTCTGATACGTCTGACAGAACAGAGGTCATTCTCAAGATTGATGGTGGTGGTTCTTCAGCTGTCTCCAGATCTCTGGACTCTGCTGACAATAATGGCGGGAAGGTTTGGCGGGAATCCAGGTACGATTTCTGGAACAATGATGAAATTGGAATTGGGGAGAGTGAGAGAATGGGTGATAATAGTGGTGTTGATAGAAACGAGGGATTTGAATTTGTACAGACTGGTTATGGAATGGAGGATCCTCCTACGAAGCTGATTGGTGACTTTCTTCGTAAGCAGAAAATTGAGGGACAAACGACTTTGGATATGGATTTGGAAATGGAGGAATTGAAGCCAAATAGGATTATAATTCCATTGGCAGAGTCGCCTTTGAGTCAAACTTCTAAGGATCTTAAAGTTTCGTTTCAGCAGGATTCGACAGAAATTTCGAGCAATGATCAGTCAATGAGAAGGCGGAATAGAGATTCTAATGATTTGAAAGAAGAGTCTAAAGGAGGACAATCGCCATGGCAACAACCGCACCATGAACGTCTTGGATCTCCAACTATCGCTGGGGTTCAGAACGAATCTCTTGCTGAGGCTATGAGATGCGCATCTAACTTGTCCTTTCATAGTGAGCTTTCATTTCAAAGGAAATCTAATTTACTGAGGACGAAAACCAAGTCAAGATTGATAGACCCACCTGCCGAGCCTGACCGGCTCTCTGGCCTCATTCCTAAGTCAGGTCAACTACGATCTGGGTTTCTGGGGAAGATTGAAGACGACGACGATGACCCCTTTCTAGAGGATGACCTTCCAGACGATTTTAAAAGGGGAAATTTCAGTGCTCTAACTGTTCTACAATGGGTTAGTTTGATTATAATCACTGCAGCTTTAATCTGCACTCTTTCTGTTCCTTATTTGAGGGAAAAGAGTCTATGGGAGTTGGATATATGGAAATGGGAGGTGATGATTATGATACTGATTTGTGGACGATTGGTATCTGGTTGGGGGATTAGGATTGGTGTATTTTTCATTGAGAGGAATTTTCTTTTGCGTAAAAGGGTTTTGTATTTTGTATATGGGGTTAGAAAACCAGTGCAGAATTGTTTATGGTTAGGCCTTGTTCTCATCGCTTGGCATTTGTTGTTCAATAAACGGGTTGAGAAACAAACCAACACCAGTATACTCAATTATGTGAGCAGAGTTTTAGTTTGTCTCTTGATAAGCACACTGATATGGCTTGTGAAAACCTTGATGGTGAAGGTGCTTGCCTCTTCTTTCCATGTAAGCACATACTTCGATCGAATTCAGGAATCGTTGTTTAATCAATATGTCATTGAGACGCTCTCGGGTCCACCTCTGGTTGAAATACGGAAGAATGAGGAAGAAGAGGAGAGGATTGCAGATGAAGTCCAAAAGTTACAGAATGCAGGAATTACCATACCCCCTGATCTCAAGGCTGCAACCTTTGCTTCTATAAAGAGTGGAAGGGCAATAGGTAGTGGACGGACCCACAAAAGTTTTTGTGCAAAAAGTTGCAAACTCTCTCGAGCACTGACCAAAAATGGGAACGATGGAATAACGATTGACCATTTGCACAAACTAAGTACGAAGAACGTCTCTGCCTGGAATATGAAGAGGTTGTTGAACATAGTTCGATATGGTAGTATTTCCACACTGGATGAACAAATACGGGGGCCGTGTCTTGATGATGAATCTACTACAGAGATCAAAAGTGAACGCGAGGCAAAGGCTGCAGCAAAAAAGATTTTTCAGAATGTGGCTCGTCGTGGATATAAGTATGTTGTTATATCTATTATATCGACACTCATGTGGGCTTGAAAATTTGTTGAAGCCCTAACATGTGGAATTTAATATCAATTGGCAAGAAAGTAATTTTAAGGGTTCAAACTCATGACCTCCACCTGTTAAATCAACAACAATTCAAAAGTTTAAGTTGACGAGTTAGGGTAAATGTAATATTATATCAATACTCTTAAATATGCCATTTCTGCTCATATTAAGCTTTCTTATGACTCATATTTTATGAAACCAACCCTCCTAATCACGTCTCCATGTATGTTTCTTGTTGCTTGACAAAGTGAAATTCTTTTGAGAATAAGTCTAAATTCAGGTTAACCACTACTTAGGTTTAATTATCTTAAGAGAACTTTTGATACTCAAATATTATAGAGATTAGCCGAGATACTTATAGGTTAGGTTTGCACACTTACATATATTTAAAAAAAAAGACATGCATTGTTATGTTTACGCTAATCTCATTACATAATTTCTTTTGATTTTGTTCTTTGCAGGTACATCTACCTGGATGACTTAGTGCGGTTCATGAGAGAAGACGAGGTTCTTAAAACGATGAGTCTCTTTGAAGGAGCCGCTGAAAACCAGAGGATAAGCAAATCTGCCTTGAAAAATTGGGTGGTAAGAATGCAAAAATGATTTCTGAAAACATTCCTCATCTCTTTTGAGTTAGTGCCATTGGTCATGTTACAAGTAAATGAAGTTACTGAGGTGAAATAGTATGACTAGCACTGTGCTGGGAAATTTGTTTAGAAATTCTGTTTACATCTAGATAATTAAAACCTCTGCAGCCAGAAAATTTAAGAATTGTTGTCTGAAATTATGGAGATAAATTAATCTTAATGAAGCTTTGTCCGAAATTATGCTCCAAAATACTCCAAATGTTATCCATGATCGTCCCAACTTCCAATTTCTGATCAAATTTCTTCCAATTCTAGAGCTTTAGCATTTTCACCGCCTATTTTTGACATTATGATGCATCCTTCTGTCCAGGTCAATGCGTTCAGGGAACGGAGAGCCCTTGCTTTAACACTGAATGATACCAAAACAGCTGTGGATAAACTACATCACATGGTGAATGTCATATTCGGCATCCTTATATTAATTTTATGGCTTATAGTATTAGGAATTGCCTCCAGCAAATTTTTCATCTTCCTTAGTTCTCAAATAGTGGTTGTGGCATTTATTTTTGGAAACACTTGCAAAACCATATTTGAAGCAATCATCTTTTTGTTTGTCATGCATCCATTTGACGTTGGAGATCGCTGCGAAATCGATGGAATGCAGGTAGCTCTCATTTGTTTTGATTTTTATGTAACTCTTGTTTCTTCTGAACCTTGGACCTGATTATGATCCCACTGAAGTGTAGATGGTTGTAGAGGAAATGAACATCTTGACTACTGTATTCTTGAGATACGACAACTTGAAGGTTATAATCCCAAATAGTGTTCTTGCAACCAAATTAATCCACAACTTCTACCGTAGTCCTGACATGGGCGAATCGGTTGAATTCCTTGTCCATATCGCTACACCAGCTGAGAAAATTACGGTCATGAAACAGAGAATTATAAGGTAATTGTTAGCTGTAAAAGAACAATGTTGTTATCTGTCTTTGACATACGTAATAGCTGACTATTTTTCTTCTGCAGTTACATTGAAGGCAACAAAGAACATTGGTATCCTGCTCCTATGATTGTGTTCAAGGATATAGATGGTTTAAACAAGGTCAAATTGGCAGTTTGGTTGTCACACAGAATGAACCATCAAGACTCAGGGGAAAGATGGGCTCGGAGGTCCGTCTTGGTCGAAGAAGTTGTTAAAGTCTGTCAAGAGCTCGACATTCAATATCGTCTATTGCCAATTGATATCAATATCCGTTCATTGCCTTCTTCTGCACCCTCCATTGGCTTTCCTTCAAATTGGACTTCCCCTGCAAGTTGA

mRNA sequence

ATGGATACGCTCAAGAAATCTTTCAAAGGCAATGTATCTTTCAAGCATACAAGGAAAATTTCTGCTGGAGGAGCCGGCAGCGAAATCAATCACGAAGAGCTCCCCATTCTTCTCAATCACCAAACTACGGATCATAGTCGTTGTCTAAGGGATCCTCATCCGGTGAACGATTCTGATACGTCTGACAGAACAGAGGTCATTCTCAAGATTGATGGTGGTGGTTCTTCAGCTGTCTCCAGATCTCTGGACTCTGCTGACAATAATGGCGGGAAGGTTTGGCGGGAATCCAGGTACGATTTCTGGAACAATGATGAAATTGGAATTGGGGAGAGTGAGAGAATGGGTGATAATAGTGGTGTTGATAGAAACGAGGGATTTGAATTTGTACAGACTGGTTATGGAATGGAGGATCCTCCTACGAAGCTGATTGGTGACTTTCTTCGTAAGCAGAAAATTGAGGGACAAACGACTTTGGATATGGATTTGGAAATGGAGGAATTGAAGCCAAATAGGATTATAATTCCATTGGCAGAGTCGCCTTTGAGTCAAACTTCTAAGGATCTTAAAGTTTCGTTTCAGCAGGATTCGACAGAAATTTCGAGCAATGATCAGTCAATGAGAAGGCGGAATAGAGATTCTAATGATTTGAAAGAAGAGTCTAAAGGAGGACAATCGCCATGGCAACAACCGCACCATGAACGTCTTGGATCTCCAACTATCGCTGGGGTTCAGAACGAATCTCTTGCTGAGGCTATGAGATGCGCATCTAACTTGTCCTTTCATAGTGAGCTTTCATTTCAAAGGAAATCTAATTTACTGAGGACGAAAACCAAGTCAAGATTGATAGACCCACCTGCCGAGCCTGACCGGCTCTCTGGCCTCATTCCTAAGTCAGGTCAACTACGATCTGGGTTTCTGGGGAAGATTGAAGACGACGACGATGACCCCTTTCTAGAGGATGACCTTCCAGACGATTTTAAAAGGGGAAATTTCAGTGCTCTAACTGTTCTACAATGGGTTAGTTTGATTATAATCACTGCAGCTTTAATCTGCACTCTTTCTGTTCCTTATTTGAGGGAAAAGAGTCTATGGGAGTTGGATATATGGAAATGGGAGGTGATGATTATGATACTGATTTGTGGACGATTGGTATCTGGTTGGGGGATTAGGATTGGTGTATTTTTCATTGAGAGGAATTTTCTTTTGCGTAAAAGGGTTTTGTATTTTGTATATGGGGTTAGAAAACCAGTGCAGAATTGTTTATGGTTAGGCCTTGTTCTCATCGCTTGGCATTTGTTGTTCAATAAACGGGTTGAGAAACAAACCAACACCAGTATACTCAATTATGTGAGCAGAGTTTTAGTTTGTCTCTTGATAAGCACACTGATATGGCTTGTGAAAACCTTGATGGTGAAGGTGCTTGCCTCTTCTTTCCATGTAAGCACATACTTCGATCGAATTCAGGAATCGTTGTTTAATCAATATGTCATTGAGACGCTCTCGGGTCCACCTCTGGTTGAAATACGGAAGAATGAGGAAGAAGAGGAGAGGATTGCAGATGAAGTCCAAAAGTTACAGAATGCAGGAATTACCATACCCCCTGATCTCAAGGCTGCAACCTTTGCTTCTATAAAGAGTGGAAGGGCAATAGGTAGTGGACGGACCCACAAAAGTTTTTGTGCAAAAAGTTGCAAACTCTCTCGAGCACTGACCAAAAATGGGAACGATGGAATAACGATTGACCATTTGCACAAACTAAGTACGAAGAACGTCTCTGCCTGGAATATGAAGAGGTTGTTGAACATAGTTCGATATGGTAGTATTTCCACACTGGATGAACAAATACGGGGGCCGTGTCTTGATGATGAATCTACTACAGAGATCAAAAGTGAACGCGAGGCAAAGGCTGCAGCAAAAAAGATTTTTCAGAATGTGGCTCGTCGTGGATATAAGTACATCTACCTGGATGACTTAGTGCGGTTCATGAGAGAAGACGAGGTTCTTAAAACGATGAGTCTCTTTGAAGGAGCCGCTGAAAACCAGAGGATAAGCAAATCTGCCTTGAAAAATTGGGTGGTCAATGCGTTCAGGGAACGGAGAGCCCTTGCTTTAACACTGAATGATACCAAAACAGCTGTGGATAAACTACATCACATGGTGAATGTCATATTCGGCATCCTTATATTAATTTTATGGCTTATAGTATTAGGAATTGCCTCCAGCAAATTTTTCATCTTCCTTAGTTCTCAAATAGTGGTTGTGGCATTTATTTTTGGAAACACTTGCAAAACCATATTTGAAGCAATCATCTTTTTGTTTGTCATGCATCCATTTGACGTTGGAGATCGCTGCGAAATCGATGGAATGCAGATGGTTGTAGAGGAAATGAACATCTTGACTACTGTATTCTTGAGATACGACAACTTGAAGGTTATAATCCCAAATAGTGTTCTTGCAACCAAATTAATCCACAACTTCTACCGTAGTCCTGACATGGGCGAATCGGTTGAATTCCTTGTCCATATCGCTACACCAGCTGAGAAAATTACGGTCATGAAACAGAGAATTATAAGTTACATTGAAGGCAACAAAGAACATTGGTATCCTGCTCCTATGATTGTGTTCAAGGATATAGATGGTTTAAACAAGGTCAAATTGGCAGTTTGGTTGTCACACAGAATGAACCATCAAGACTCAGGGGAAAGATGGGCTCGGAGGTCCGTCTTGGTCGAAGAAGTTGTTAAAGTCTGTCAAGAGCTCGACATTCAATATCGTCTATTGCCAATTGATATCAATATCCGTTCATTGCCTTCTTCTGCACCCTCCATTGGCTTTCCTTCAAATTGGACTTCCCCTGCAAGTTGA

Coding sequence (CDS)

ATGGATACGCTCAAGAAATCTTTCAAAGGCAATGTATCTTTCAAGCATACAAGGAAAATTTCTGCTGGAGGAGCCGGCAGCGAAATCAATCACGAAGAGCTCCCCATTCTTCTCAATCACCAAACTACGGATCATAGTCGTTGTCTAAGGGATCCTCATCCGGTGAACGATTCTGATACGTCTGACAGAACAGAGGTCATTCTCAAGATTGATGGTGGTGGTTCTTCAGCTGTCTCCAGATCTCTGGACTCTGCTGACAATAATGGCGGGAAGGTTTGGCGGGAATCCAGGTACGATTTCTGGAACAATGATGAAATTGGAATTGGGGAGAGTGAGAGAATGGGTGATAATAGTGGTGTTGATAGAAACGAGGGATTTGAATTTGTACAGACTGGTTATGGAATGGAGGATCCTCCTACGAAGCTGATTGGTGACTTTCTTCGTAAGCAGAAAATTGAGGGACAAACGACTTTGGATATGGATTTGGAAATGGAGGAATTGAAGCCAAATAGGATTATAATTCCATTGGCAGAGTCGCCTTTGAGTCAAACTTCTAAGGATCTTAAAGTTTCGTTTCAGCAGGATTCGACAGAAATTTCGAGCAATGATCAGTCAATGAGAAGGCGGAATAGAGATTCTAATGATTTGAAAGAAGAGTCTAAAGGAGGACAATCGCCATGGCAACAACCGCACCATGAACGTCTTGGATCTCCAACTATCGCTGGGGTTCAGAACGAATCTCTTGCTGAGGCTATGAGATGCGCATCTAACTTGTCCTTTCATAGTGAGCTTTCATTTCAAAGGAAATCTAATTTACTGAGGACGAAAACCAAGTCAAGATTGATAGACCCACCTGCCGAGCCTGACCGGCTCTCTGGCCTCATTCCTAAGTCAGGTCAACTACGATCTGGGTTTCTGGGGAAGATTGAAGACGACGACGATGACCCCTTTCTAGAGGATGACCTTCCAGACGATTTTAAAAGGGGAAATTTCAGTGCTCTAACTGTTCTACAATGGGTTAGTTTGATTATAATCACTGCAGCTTTAATCTGCACTCTTTCTGTTCCTTATTTGAGGGAAAAGAGTCTATGGGAGTTGGATATATGGAAATGGGAGGTGATGATTATGATACTGATTTGTGGACGATTGGTATCTGGTTGGGGGATTAGGATTGGTGTATTTTTCATTGAGAGGAATTTTCTTTTGCGTAAAAGGGTTTTGTATTTTGTATATGGGGTTAGAAAACCAGTGCAGAATTGTTTATGGTTAGGCCTTGTTCTCATCGCTTGGCATTTGTTGTTCAATAAACGGGTTGAGAAACAAACCAACACCAGTATACTCAATTATGTGAGCAGAGTTTTAGTTTGTCTCTTGATAAGCACACTGATATGGCTTGTGAAAACCTTGATGGTGAAGGTGCTTGCCTCTTCTTTCCATGTAAGCACATACTTCGATCGAATTCAGGAATCGTTGTTTAATCAATATGTCATTGAGACGCTCTCGGGTCCACCTCTGGTTGAAATACGGAAGAATGAGGAAGAAGAGGAGAGGATTGCAGATGAAGTCCAAAAGTTACAGAATGCAGGAATTACCATACCCCCTGATCTCAAGGCTGCAACCTTTGCTTCTATAAAGAGTGGAAGGGCAATAGGTAGTGGACGGACCCACAAAAGTTTTTGTGCAAAAAGTTGCAAACTCTCTCGAGCACTGACCAAAAATGGGAACGATGGAATAACGATTGACCATTTGCACAAACTAAGTACGAAGAACGTCTCTGCCTGGAATATGAAGAGGTTGTTGAACATAGTTCGATATGGTAGTATTTCCACACTGGATGAACAAATACGGGGGCCGTGTCTTGATGATGAATCTACTACAGAGATCAAAAGTGAACGCGAGGCAAAGGCTGCAGCAAAAAAGATTTTTCAGAATGTGGCTCGTCGTGGATATAAGTACATCTACCTGGATGACTTAGTGCGGTTCATGAGAGAAGACGAGGTTCTTAAAACGATGAGTCTCTTTGAAGGAGCCGCTGAAAACCAGAGGATAAGCAAATCTGCCTTGAAAAATTGGGTGGTCAATGCGTTCAGGGAACGGAGAGCCCTTGCTTTAACACTGAATGATACCAAAACAGCTGTGGATAAACTACATCACATGGTGAATGTCATATTCGGCATCCTTATATTAATTTTATGGCTTATAGTATTAGGAATTGCCTCCAGCAAATTTTTCATCTTCCTTAGTTCTCAAATAGTGGTTGTGGCATTTATTTTTGGAAACACTTGCAAAACCATATTTGAAGCAATCATCTTTTTGTTTGTCATGCATCCATTTGACGTTGGAGATCGCTGCGAAATCGATGGAATGCAGATGGTTGTAGAGGAAATGAACATCTTGACTACTGTATTCTTGAGATACGACAACTTGAAGGTTATAATCCCAAATAGTGTTCTTGCAACCAAATTAATCCACAACTTCTACCGTAGTCCTGACATGGGCGAATCGGTTGAATTCCTTGTCCATATCGCTACACCAGCTGAGAAAATTACGGTCATGAAACAGAGAATTATAAGTTACATTGAAGGCAACAAAGAACATTGGTATCCTGCTCCTATGATTGTGTTCAAGGATATAGATGGTTTAAACAAGGTCAAATTGGCAGTTTGGTTGTCACACAGAATGAACCATCAAGACTCAGGGGAAAGATGGGCTCGGAGGTCCGTCTTGGTCGAAGAAGTTGTTAAAGTCTGTCAAGAGCTCGACATTCAATATCGTCTATTGCCAATTGATATCAATATCCGTTCATTGCCTTCTTCTGCACCCTCCATTGGCTTTCCTTCAAATTGGACTTCCCCTGCAAGTTGA

Protein sequence

MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDTSDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIIIPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHERLGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPPDLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS*
Homology
BLAST of Chy1G006540 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 951.0 bits (2457), Expect = 9.9e-276
Identity = 517/931 (55.53%), Postives = 656/931 (70.46%), Query Frame = 0

Query: 59  DTSDRTEVILKIDGGGSSAVSRSLDSADNNG------GKVWRESRYDFWNNDEIGIG--- 118
           D +DR EVI+KIDG          ++ +NNG      GK+WR+  YDFW + E  +    
Sbjct: 4   DAADRREVIVKIDG----------ENGNNNGVSGETVGKIWRDGSYDFWTDGEGNLNKGH 63

Query: 119 -----ESERMGDNSG-VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLE 178
                +S+R    +G   ++EGFEF +     EDPPTKLIG FL KQ+  G+  LDMDL 
Sbjct: 64  NAAAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLG 123

Query: 179 MEELKPNRIIIPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGG 238
           M+EL+ +R + P++ESP   T +D                  + RR+  SN         
Sbjct: 124 MDELQ-SRGLTPVSESPRVSTKRD-----------------PVGRRDSRSN--------- 183

Query: 239 QSPWQQPHHERLGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSN-LLRTKTKSRLI 298
                                N    E ++C+ N         QR S+ LL+ +T+SRL 
Sbjct: 184 -------------------TNNNDDGEVVKCSGN-----NAPIQRSSSTLLKMRTRSRLS 243

Query: 299 DPPA------EPDRLSGLIPKSGQLRSGFLGKI-----EDDDDDPFLEDDLPDDFKRGNF 358
           DPP         D  SG IPKSGQ++SGF GK      E+++DDPF  +DLP+++++   
Sbjct: 244 DPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKL 303

Query: 359 SALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRI 418
           S   VL+W+SLI+I A  +CTL++P LR+K LWEL +WKWE M+++LICGRLVS W ++I
Sbjct: 304 SLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKI 363

Query: 419 GVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVS 478
            VFFIERNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+++V K  NT  L  V+
Sbjct: 364 VVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVT 423

Query: 479 RVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKN 538
           ++ VCLL+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+KN
Sbjct: 424 KIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKN 483

Query: 539 EEEEERIADEVQKLQNAGITIPPDLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSRALT 598
           EEEEERI+ EV+K QN G              I+SG      +T      KS  LS  L+
Sbjct: 484 EEEEERISVEVKKFQNPG-----------GVEIQSGAQKSPMKT-----GKSPFLSHVLS 543

Query: 599 KNG-----NDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT 658
             G     N GITID LHKL+ KNVSAW MKRL+NI+R GS++TLDEQ++ P LDD+   
Sbjct: 544 NGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGN 603

Query: 659 EIKSEREAKAAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKS 718
           +I+SE EAK AA+KIF NVA+ G K+IY +D++RF+ +DE LKT+SLFEGA+E  RISKS
Sbjct: 604 QIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKS 663

Query: 719 ALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIF 778
           +LKNWVVNAFRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+LGI S+KF + 
Sbjct: 664 SLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVV 723

Query: 779 LSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRY 838
           +SSQ+VVVAFIFGN CK +FE+II+LFV+HPFDVGDRCEIDG+QMVVEEMNILTTVFLR+
Sbjct: 724 MSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRF 783

Query: 839 DNLKVIIPNSVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEH 898
           DN KV+ PNS+L TK I N+YRSPDMG+ +EF +HI TPAEKI ++KQRI SYIEG K+H
Sbjct: 784 DNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDH 843

Query: 899 WYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 955
           WYPAPMIVFKD++ LN V++AVW +HRMNHQD GE+WARRS LVEE+ K+C+ELDI+YRL
Sbjct: 844 WYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRL 853

BLAST of Chy1G006540 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 3.4e-260
Identity = 487/926 (52.59%), Postives = 653/926 (70.52%), Query Frame = 0

Query: 59  DTSDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNS 118
           D++DR + I+ I+G  S AV  +  S++  GG +W+ES YDFW+ ++   G++++ GD+ 
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGEK---GKNDKKGDDE 64

Query: 119 GVDRNEGFEFVQTG------YGMEDPPTKLIGDFLRKQKIEG-QTTLDMDLEMEELKPNR 178
             D    F F Q G        + DPP+KLIG FL KQ+  G + +LD++L M EL+ N 
Sbjct: 65  DED-GGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN- 124

Query: 179 IIIPLAESPLSQTSKDLKVSFQQDSTEISSN---DQSMRRRNRDSNDLKEESKGGQSPWQ 238
              P   +  S T +    +  + S+ + +    D   RR+NR S               
Sbjct: 125 --TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTS--------------- 184

Query: 239 QPHHERLGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPA-- 298
                 LG  +    +N   AE ++C S           +K  L R KTKSRL DPP   
Sbjct: 185 ------LGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPT 244

Query: 299 -----EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLPDDFKRG 358
                + +  SG   +SG  +SGFLGK             E++++DPFL++DLP++FKR 
Sbjct: 245 HPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRD 304

Query: 359 NFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGI 418
             S    L+W+SL++I  +L+C+L++  L+ K+ W+LD+WKWEV +++LICGRLVS W +
Sbjct: 305 KLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIV 364

Query: 419 RIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNY 478
           RI VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T ++ L Y
Sbjct: 365 RIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRY 424

Query: 479 VSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIR 538
           V+RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+
Sbjct: 425 VTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQ 484

Query: 539 KNEEEEERIADEVQKLQN-AGITIPPDLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSR 598
           + EEEE+++A++V+ L+  AG  +PP LKA   + +K G++ G              L+R
Sbjct: 485 RMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPG--------------LNR 544

Query: 599 ALTKNGND--GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT 658
             +K G D  GI ID L +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    +DE  T
Sbjct: 545 IGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDAT 604

Query: 659 EIKSEREAKAAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKS 718
            I+SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+ +ISKS
Sbjct: 605 HIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKS 664

Query: 719 ALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIF 778
            LKNWVV AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++F + 
Sbjct: 665 CLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLV 724

Query: 779 LSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRY 838
           LSSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILTTVFLRY
Sbjct: 725 LSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRY 784

Query: 839 DNLKVIIPNSVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEH 898
           DN K+I PNSVL TK I N+YRSPDMG++VEF VHIATP EKIT +KQRI+SY++  K++
Sbjct: 785 DNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDY 844

Query: 899 WYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 950
           WYPAPMIVF  +D LN VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+ELDI+YRL
Sbjct: 845 WYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRL 875

BLAST of Chy1G006540 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 894.8 bits (2311), Expect = 8.4e-259
Identity = 500/978 (51.12%), Postives = 671/978 (68.61%), Query Frame = 0

Query: 5   KKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDTSDRT 64
           + SFK + S+K   +I + G  SE + E LPIL +H   DHS                  
Sbjct: 4   RNSFKSHSSYK---QIRSPGDQSEPSPEHLPILHDHH-PDHSG----------------- 63

Query: 65  EVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESE---RMGDNSGV- 124
              + +D     +   SLD  D     V R++ Y FW ++  G        R  D   + 
Sbjct: 64  ---MVVDDQKPDSTRSSLD--DGRNAPVERDASYKFWQDNTTGTSTDHTAVRTSDKDPIA 123

Query: 125 -----DRNEG-FEFVQTGYGMEDPPTKLI-GDFLRKQ---KIEGQTTLDMDLEMEELKPN 184
                DR  G F+FV     +++ PTK++ G+ + +Q   +   + TLD+D E +++   
Sbjct: 124 ISRKGDRLSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQENDDVSHQ 183

Query: 185 RIIIPLAESPLS-QTSKDLKVSFQ--------------QDSTEISSNDQSMRRRNRDSND 244
            +  P + +  S   S++++VSF                 S+  SS+  +  R N+D   
Sbjct: 184 TMPTPTSTARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSATMRTNQDQPQ 243

Query: 245 LKEESKGGQSPWQQPHHERLGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLL-R 304
           L+EE                              E +RC SN      +SFQRKS L+ R
Sbjct: 244 LQEE------------------------------EVVRCTSN------MSFQRKSELISR 303

Query: 305 TKTKSRLIDPPAEPDR-LSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSA 364
            KT+SRL DPP E +   SG   +SGQL+SG L  I D++DDP  E+D+PD++KRG   A
Sbjct: 304 VKTRSRLQDPPREEETPYSGW--RSGQLKSGLLADI-DEEDDPLAEEDVPDEYKRGKLDA 363

Query: 365 LTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGV 424
           +T+LQW+SL+ I AAL C+LS+   ++  +W L +WKWEV +++LICGRLVSGWGIRI V
Sbjct: 364 ITLLQWLSLVAIIAALACSLSIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVV 423

Query: 425 FFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRV 484
           FFIERNFLLRKRVLYFVYGVR+ VQNCLWLGLVL+AWH LF+K+V+++T +  L YV+++
Sbjct: 424 FFIERNFLLRKRVLYFVYGVRRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKI 483

Query: 485 LVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEE 544
           LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LFNQYVIETLSGPP++E+ + EE
Sbjct: 484 LVCFLLSTILWLIKTLVVKVLASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEE 543

Query: 545 EEERIADEVQKLQNAGITIPPDLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKN 604
           EEER  DE+ K+QNAG  +PPDL AA F   KSGR +              KLS  + K+
Sbjct: 544 EEERAQDEIFKMQNAGANLPPDLCAAAFPPGKSGRVMNP------------KLSPIIPKS 603

Query: 605 GND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSER 664
             D GI+++HLH+++ KN+SAWNMKRL+ IVR  S++TLDEQ+     +DEST +I+SE+
Sbjct: 604 TTDNGISMEHLHRMNHKNISAWNMKRLMKIVRNVSLTTLDEQMLESTYEDESTRQIRSEK 663

Query: 665 EAKAAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWV 724
           EAKAAA+KIF+NV +RG KYIYL+DL+RF+REDE +KTM LFEGA EN+RISKSALKNW+
Sbjct: 664 EAKAAARKIFKNVEQRGAKYIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWL 723

Query: 725 VNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIV 784
           VNAFRERRALALTLNDTKTAV+KLHHM+N++  I+I+++WL++L IASSK  +F+SSQ+V
Sbjct: 724 VNAFRERRALALTLNDTKTAVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVV 783

Query: 785 VVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVI 844
           ++AFIFGNT KT+FE+IIFLF++HP+DVGDRCEID +Q+VVEEMNILTTVFLRYDNLK++
Sbjct: 784 LLAFIFGNTVKTVFESIIFLFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIM 843

Query: 845 IPNSVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPM 904
            PNS+L  K I+N+YRSPDMG+++EF VHI TP EKI+V+KQRI +YI+   E+WYP   
Sbjct: 844 YPNSLLWQKSINNYYRSPDMGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAK 903

Query: 905 IVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDIN 951
           I+ KD++ L+ V+LA+W  HR+NHQD  ERW RR+VLVEEV+K+  ELDIQ+R  P+DIN
Sbjct: 904 IIVKDLEDLHIVRLAIWPCHRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDIN 903

BLAST of Chy1G006540 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 849.7 bits (2194), Expect = 3.1e-245
Identity = 462/921 (50.16%), Postives = 643/921 (69.82%), Query Frame = 0

Query: 60  TSDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNSG 119
           T  R + +++IDG          D+ D+   K WRES  +FW+ND+     S+  G   G
Sbjct: 6   TDQRRDFVVRIDGE---------DNGDSE--KFWRESSINFWHNDK----SSKPPG---G 65

Query: 120 VDRNEGFEFVQTGYGME---DPPTKLIGDFLRKQKIEG-QTTLDMDLEMEELKPNRIIIP 179
            + +  F+F++         DPP+KLI  FL KQK  G + +LDM+  M EL+ N  + P
Sbjct: 66  EEDDGSFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKN-TVPP 125

Query: 180 LAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHERL 239
           L+ + +S ++  +       S    + D   RR+NR +  L    K G S   +    R+
Sbjct: 126 LSSTAVSGSASPVTAPVTA-SYRNGTGDAIRRRQNRVT--LSPSVKDGDS--SEDEENRV 185

Query: 240 GSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAE--PDRLSG 299
                        +E ++C SN       S  R   L++ KT+SRL+DPP    PD +SG
Sbjct: 186 DG-----------SEVVKCTSN------RSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSG 245

Query: 300 LIPKSGQLRSGFLG-------------KIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWV 359
             P+SG L  GF G             K  ++++DPF E+DLP+  ++       +++W+
Sbjct: 246 RTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWI 305

Query: 360 SLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNF 419
            LI+I A+LIC+L +PYLR K+LW+L +WKWEVM+++LICGRLVS W +++ V+F+E NF
Sbjct: 306 FLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNF 365

Query: 420 LLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLIS 479
           L RK+VLYFVYG+RKPVQNCLWLGLVLIAWH LF+K+VE++  +++L YV++VL+CLL++
Sbjct: 366 LWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVA 425

Query: 480 TLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIAD 539
            +IWL+KTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPP +EI     EEE++A+
Sbjct: 426 VIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---HIEEEKVAN 485

Query: 540 EVQKLQNAGITIPPDLKAATFASIKSGRAIGSGRTHK--SFCAKSCKLSRALTK--NGND 599
           +V+  +  G  + P        S      +GSGR  K  S   KS  LSR+ +K   G +
Sbjct: 486 DVKTFEIVGRKLSP--LGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEE 545

Query: 600 GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCL-DDESTTEIKSEREAK 659
           GI IDHL +++TKNVSAW MK+L+N+++ G++STLDEQI+     +D+  T+I+SE EAK
Sbjct: 546 GIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAK 605

Query: 660 AAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNA 719
            AA+KIFQNVA  G +YIY++D +RF+ EDE  + M LFEGA+E  +ISKS LKNWVVNA
Sbjct: 606 LAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNA 665

Query: 720 FRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVA 779
           FRERRALALTLNDTKTAV++LH +V+V+  I+ILI+WL++LGIA++KF + +SSQ+++V 
Sbjct: 666 FRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVV 725

Query: 780 FIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPN 839
           F+FGN+CKTIFEA+IF+FVMHPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ PN
Sbjct: 726 FVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPN 785

Query: 840 SVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVF 899
           S+L TK I N+YRSPDM +++EF VHIATP EK T ++QRI+SY++  K+HW+P+PMIVF
Sbjct: 786 SLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVF 845

Query: 900 KDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRS 955
           +D+ GLN VK+A+W +H+MNHQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN++S
Sbjct: 846 RDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKS 880

BLAST of Chy1G006540 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 1.6e-225
Identity = 399/689 (57.91%), Postives = 534/689 (77.50%), Query Frame = 0

Query: 268 RKSNLL-RTKTKSRLIDPPAEPD-RLSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 327
           RK+ L+ R K +SRLIDPP E + + S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 328 PDDFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGR 387
           P ++++    A+T+LQW+SLI +  AL+ +L +   R  +LW L +WKWEV++++LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 388 LVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 447
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 448 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 507
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 508 PPLVEIRKNEEEEERIADEVQKLQNAGITIPPDLKAATFASIKSGRAIGSGRTHKSFCAK 567
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L +A F   KSG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 568 SCKLSRALTKNGND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 627
           + K S  + K G+D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 628 DESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQ 687
           DEST +I+SE+EAKAAA+KIF+NVA+ G K+IYL+DL+RF+R DE +KTM LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 688 RISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 747
           +I+KSALKNW+VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 748 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTT 807
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 808 VFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIE 867
           VFLR DNLK++ PN +L  K IHN+ RSPDMG+ V   VHI TP EKI  +KQRI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 868 GNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELD 927
              E+WYP   ++ KD++ LN V++A+WL H++NHQ+ GER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 928 IQYRLLPIDINIRSLPSSAPSIGFPSNWT 951
           IQYR  P+DIN++++P+   S   P  W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSS-RVPPAWS 836

BLAST of Chy1G006540 vs. ExPASy TrEMBL
Match: A0A0A0K619 (Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_7G017150 PE=3 SV=1)

HSP 1 Score: 1809.3 bits (4685), Expect = 0.0e+00
Identity = 932/955 (97.59%), Postives = 938/955 (98.22%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRD HPVNDSDT
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDGGG-SSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNSG 120
           SDRTEVILKIDGGG SSAVSRSLDSA NNGG VWRESRYDFWNNDEIGIGES RMGDNSG
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIIIPLAES 180
           VDRNEGFEFVQTGYGMEDPPTKLIGDFL KQKIEG+TTLDMDLEMEELKPNRI+ PLAES
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180

Query: 181 PLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHERLGSPT 240
           PLSQTSKDLKVSFQ DSTEISSNDQS+RRRNRDSNDLKEESKGGQSP QQPHHERLGSPT
Sbjct: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240

Query: 241 IAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGLIPKSG 300
           I+GVQNESLAEAMRCASNLSFHSELSFQRKSNLLR KTKSRLIDPPAEPDRLSGLIPKSG
Sbjct: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300

Query: 301 QLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360
           QLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNFSALTVLQWVSLIIITAALICTLSVPYLR
Sbjct: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360

Query: 361 EKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420
           EKSLWELDIWKWEVMI ILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN
Sbjct: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420

Query: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480
           CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH
Sbjct: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480

Query: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPPDLKAA 540
           VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGI IPPDLKAA
Sbjct: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540

Query: 541 TFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAWNMKRL 600
           TFASIKSGR IGSGRTHKSFCAKSCKLSRALTKN NDGITIDHLHKLSTKNVSAWNMKRL
Sbjct: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600

Query: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLV 660
           LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYIYLDDLV
Sbjct: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660

Query: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720
           RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM
Sbjct: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720

Query: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780
           VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD
Sbjct: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780

Query: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFL 840
           VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES+EFL
Sbjct: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840

Query: 841 VHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900
           VHIATPAEKIT MK RIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS
Sbjct: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900

Query: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955
           GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955

BLAST of Chy1G006540 vs. ExPASy TrEMBL
Match: A0A1S3BID3 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490400 PE=3 SV=1)

HSP 1 Score: 1714.9 bits (4440), Expect = 0.0e+00
Identity = 894/960 (93.12%), Postives = 907/960 (94.48%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60

Query: 61  SDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGES------ERM 120
           SDRTEVILKID GGSSAVSRSLDS  NNGGKVWRESRYDFWNNDE GIGES       RM
Sbjct: 61  SDRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARM 120

Query: 121 GDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIII 180
            D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI G+TTLDMDLEMEELKPNRII 
Sbjct: 121 SD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIP 180

Query: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHER 240
           PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRR RDS+DLKEE KG Q PWQQ HHER
Sbjct: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHER 240

Query: 241 LGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGL 300
           LGSPTI+GVQNESLAEAMRCASNLSFHSELSFQRKSNLLR KTKSRL DPPAEPDRLSGL
Sbjct: 241 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGL 300

Query: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLS 360
           IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNF+ALTVLQW SLI+ITAA ICTLS
Sbjct: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLS 360

Query: 361 VPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVR 420
           VPYLREKSLWELDIWKWEVMI+ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVR
Sbjct: 361 VPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVR 420

Query: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480
           KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL
Sbjct: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+TIPP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPP 540

Query: 541 DLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAW 600
           DLK ATFASIKSGRAI S RTHKSFCAKS K SRALTKNGNDGITIDHLHKLS KNVSAW
Sbjct: 541 DLK-ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAW 600

Query: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660
           NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY
Sbjct: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660

Query: 661 LDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVD 720
           LDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAVD
Sbjct: 661 LDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVD 720

Query: 721 KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780
           KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV
Sbjct: 721 KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780

Query: 781 MHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGE 840
           MHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMGE
Sbjct: 781 MHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGE 840

Query: 841 SVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRM 900
           SVEF VHIATPAEKIT MKQRIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHRM
Sbjct: 841 SVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRM 900

Query: 901 NHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955
           NHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 NHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 951

BLAST of Chy1G006540 vs. ExPASy TrEMBL
Match: A0A5D3DI81 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004470 PE=3 SV=1)

HSP 1 Score: 1712.6 bits (4434), Expect = 0.0e+00
Identity = 893/960 (93.02%), Postives = 906/960 (94.38%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60

Query: 61  SDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGES------ERM 120
           SDRTEVILKID  GSSAVSRSLDS  NNGGKVWRESRYDFWNNDE GIGES       RM
Sbjct: 61  SDRTEVILKIDDSGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARM 120

Query: 121 GDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIII 180
            D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI G+TTLDMDLEMEELKPNRII 
Sbjct: 121 SD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIP 180

Query: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHER 240
           PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRR RDS+DLKEE KG Q PWQQ HHER
Sbjct: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHER 240

Query: 241 LGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGL 300
           LGSPTI+GVQNESLAEAMRCASNLSFHSELSFQRKSNLLR KTKSRL DPPAEPDRLSGL
Sbjct: 241 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGL 300

Query: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLS 360
           IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNF+ALTVLQW SLI+ITAA ICTLS
Sbjct: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLS 360

Query: 361 VPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVR 420
           VPYLREKSLWELDIWKWEVMI+ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVR
Sbjct: 361 VPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVR 420

Query: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480
           KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL
Sbjct: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+TIPP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPP 540

Query: 541 DLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAW 600
           DLK ATFASIKSGRAI S RTHKSFCAKS K SRALTKNGNDGITIDHLHKLS KNVSAW
Sbjct: 541 DLK-ATFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAW 600

Query: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660
           NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY
Sbjct: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660

Query: 661 LDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVD 720
           LDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAVD
Sbjct: 661 LDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVD 720

Query: 721 KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780
           KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV
Sbjct: 721 KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780

Query: 781 MHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGE 840
           MHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMGE
Sbjct: 781 MHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGE 840

Query: 841 SVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRM 900
           SVEF VHIATPAEKIT MKQRIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHRM
Sbjct: 841 SVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRM 900

Query: 901 NHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955
           NHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 NHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 951

BLAST of Chy1G006540 vs. ExPASy TrEMBL
Match: A0A6J1C288 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111007608 PE=3 SV=1)

HSP 1 Score: 1460.3 bits (3779), Expect = 0.0e+00
Identity = 766/960 (79.79%), Postives = 841/960 (87.60%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MDT+KKSFK NVSFKHTRKISAGG G+EI+H+ELPILL+H+     R  R    +ND D 
Sbjct: 1   MDTIKKSFKSNVSFKHTRKISAGGGGTEISHKELPILLDHEPAAVHR-HRGRRSMNDCDP 60

Query: 61  SDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNSGV 120
           SDRTEVILKID GGS+AVSR +   + NGGKVWRE+ YDFWNND  G   + R    S V
Sbjct: 61  SDRTEVILKIDDGGSAAVSRPV---EGNGGKVWRETNYDFWNNDGKGENGASRASGASRV 120

Query: 121 ---------DRNEGFEFVQTGYG--MEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKP 180
                    DRNEGFEFVQ GYG  +EDPP KLIG+FL KQK+ G+TTLDMDLEMEEL+ 
Sbjct: 121 SGARASDNGDRNEGFEFVQRGYGVEVEDPPMKLIGEFLHKQKLRGETTLDMDLEMEELQQ 180

Query: 181 NRIIIPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQ 240
           +RII PLAESPLSQTSKDLKVSFQQDSTE SSND S+RRR RDS +L++E KG QSPWQQ
Sbjct: 181 DRIIPPLAESPLSQTSKDLKVSFQQDSTESSSND-SIRRRYRDSRELQDEYKGQQSPWQQ 240

Query: 241 PHHERLGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPD 300
            HHER GSPTI+GVQN+  AEA RC SNLSF  ELSFQR S+LLR KTKSRL+DPP EP 
Sbjct: 241 SHHERHGSPTISGVQNDCAAEAARCTSNLSFQRELSFQRTSHLLRAKTKSRLMDPPEEPA 300

Query: 301 RLSGLIPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAAL 360
            LSGLIPKSG LRSGFLGK E++DDDPFLE+DLP+DFKR +FS LT+LQW+SL++IT AL
Sbjct: 301 LLSGLIPKSGPLRSGFLGKGEEEDDDPFLEEDLPEDFKRSDFSVLTLLQWISLVLITGAL 360

Query: 361 ICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYF 420
           +CTL +PYLR  SLWEL+IWKWEVM++ILICGRLVSGWGIRI VF IERNFLLRKR+LYF
Sbjct: 361 VCTLYIPYLRAMSLWELNIWKWEVMVLILICGRLVSGWGIRIIVFCIERNFLLRKRLLYF 420

Query: 421 VYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTL 480
           VYGVRKPVQNC+WLGLVLIAW LLFNKRVE +TN ++L YV RVLV LL+STLIWLVKTL
Sbjct: 421 VYGVRKPVQNCIWLGLVLIAWRLLFNKRVESETNGTVLKYVRRVLVSLLVSTLIWLVKTL 480

Query: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG 540
           MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EIRKNEE EER+ADEVQKLQNAG
Sbjct: 481 MVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEIRKNEEAEERLADEVQKLQNAG 540

Query: 541 ITIPPDLKAATFASIKSGRAIGSGRTHKSFCAKSC--KLSRALTKNGNDGITIDHLHKLS 600
           ++IPPDLKA T +S+KSGR IG G   KS   KS   K SR LTKNGNDGITIDHLHKLS
Sbjct: 541 VSIPPDLKADT-SSVKSGRVIGGGVNQKSSHGKSSHGKSSRPLTKNGNDGITIDHLHKLS 600

Query: 601 TKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAR 660
            KNVSAWNMKRLLN+VRYGSISTLDEQI GPCL+DEST EI+SEREAKAAAKKIFQNVAR
Sbjct: 601 PKNVSAWNMKRLLNMVRYGSISTLDEQIPGPCLEDESTREIRSEREAKAAAKKIFQNVAR 660

Query: 661 RGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLN 720
           R  KYIY +DL+RFMREDEVL+TMSLFEGA E++RISKS+LKNWVVNAFRERR+LALTLN
Sbjct: 661 RELKYIYPEDLMRFMREDEVLRTMSLFEGATESRRISKSSLKNWVVNAFRERRSLALTLN 720

Query: 721 DTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFE 780
           DTKTAVD+LHHMVNVIFGILILILWLI+LGIASSKFF F+SSQIVVVAFIFGNTCKTIFE
Sbjct: 721 DTKTAVDRLHHMVNVIFGILILILWLILLGIASSKFFAFVSSQIVVVAFIFGNTCKTIFE 780

Query: 781 AIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFY 840
           AIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFY
Sbjct: 781 AIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFY 840

Query: 841 RSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLA 900
           RSPDMGESVEF +HIATPAE I  MKQRIISYIEGNK HW P+PM VFKD++ LN+++LA
Sbjct: 841 RSPDMGESVEFCIHIATPAETIATMKQRIISYIEGNKAHWSPSPMFVFKDVEELNRLRLA 900

Query: 901 VWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPS 948
           VWLSHRMNHQDSGERWARRSVLVE+VVKVCQELDIQYRLLPIDIN+ SLPSSAPS+GF S
Sbjct: 901 VWLSHRMNHQDSGERWARRSVLVEQVVKVCQELDIQYRLLPIDINVHSLPSSAPSMGFTS 954

BLAST of Chy1G006540 vs. ExPASy TrEMBL
Match: A0A6J1EJZ4 (mechanosensitive ion channel protein 6-like OS=Cucurbita moschata OX=3662 GN=LOC111435046 PE=4 SV=1)

HSP 1 Score: 1296.6 bits (3354), Expect = 0.0e+00
Identity = 671/935 (71.76%), Postives = 788/935 (84.28%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MD+ KKS K N  FKH+RKISAGGAGSEIN EELPILL+HQ +D     +   P ND + 
Sbjct: 1   MDSFKKSLKHNGPFKHSRKISAGGAGSEINQEELPILLDHQPSDQHNRPKVRRPANDGEP 60

Query: 61  SDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFW-NNDEIGIGESERMGDNSG 120
           S  +EVILK+D GGSSAVSRS +SA   GGKVWRESRYDFW NND IG G S      + 
Sbjct: 61  SGHSEVILKVDDGGSSAVSRSQESA-GYGGKVWRESRYDFWSNNDGIGTGGSASWASGAR 120

Query: 121 V----DRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIIIP 180
                DRNEGFEFV+ G G +DPPTKLIGDFL KQK+ G+TTLD+DLEMEEL+ +R   P
Sbjct: 121 ASDSGDRNEGFEFVRRGNGTDDPPTKLIGDFLHKQKVRGETTLDLDLEMEELRLDRTKTP 180

Query: 181 LAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHERL 240
           + +SPLS+ SKDLKVSFQ DSTEISSN +S+RRR ++  +++EESK GQ  W Q HHE  
Sbjct: 181 MMDSPLSRASKDLKVSFQHDSTEISSN-ESVRRRYKEFREMQEESKRGQPSWHQSHHEHG 240

Query: 241 GSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGLI 300
           GS +I+GVQN+S+AEAMR ASNLSF S LS  RKS+LL+ K KSRL DPPAEPDRLSGL+
Sbjct: 241 GS-SISGVQNDSVAEAMRSASNLSFQSTLSLHRKSHLLKAKGKSRLTDPPAEPDRLSGLV 300

Query: 301 PKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLSV 360
            KSGQLRSGFLG+ +D++DDPFLE+D PDDF+RG   ALTVLQW+SL +ITAAL+CTLS+
Sbjct: 301 NKSGQLRSGFLGRTDDEEDDPFLEEDFPDDFRRGKLDALTVLQWISLFLITAALVCTLSI 360

Query: 361 PYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRK 420
            +LRE SLWE  IWKWEVM+++LICGRLVSGWGI IGVFFIERNFLLRK+VLYFVYGV++
Sbjct: 361 RHLRENSLWEFSIWKWEVMVLVLICGRLVSGWGIGIGVFFIERNFLLRKKVLYFVYGVKR 420

Query: 421 PVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLA 480
           PVQNCLWLGLVLIAWHLLF+KRV+ QTN+ IL+YV + LVCLL+ TLIWL KTLM+KVLA
Sbjct: 421 PVQNCLWLGLVLIAWHLLFDKRVKNQTNSDILDYVRKSLVCLLLGTLIWLAKTLMLKVLA 480

Query: 481 SSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPPD 540
           SSFHVSTYFDRIQESLFNQYVIETLSGPP +E++K+EEE+++IADEVQ+LQ+AG+ +PPD
Sbjct: 481 SSFHVSTYFDRIQESLFNQYVIETLSGPPRIELQKSEEEQKKIADEVQRLQSAGVPVPPD 540

Query: 541 LKAATFASIKSGRAIGSG-RTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAW 600
           L+AA  +SIKSG  + S   + +S   K  KLSR  TKNG++GI IDHLHKL++KNVSAW
Sbjct: 541 LRAAALSSIKSGERVRSNVGSQRSLRGKDPKLSRVSTKNGSEGINIDHLHKLTSKNVSAW 600

Query: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDES-TTEIKSEREAKAAAKKIFQNVARRGYKYI 660
           NMKRLL IVRYGSI+TLDEQI+GP LDDES TTEIKSEREAKAAAKKIFQNVAR G+KYI
Sbjct: 601 NMKRLLKIVRYGSITTLDEQIQGPSLDDESTTTEIKSEREAKAAAKKIFQNVARPGFKYI 660

Query: 661 YLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAV 720
           YLDDL+RFM+E+EVLKT+S FEGA E +RISKSALKNWVVNAFRERRAL+LTLNDTKTAV
Sbjct: 661 YLDDLMRFMKEEEVLKTLSRFEGATETRRISKSALKNWVVNAFRERRALSLTLNDTKTAV 720

Query: 721 DKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLF 780
           ++L  +VNVIF I IL+LW+I+LGIAS K  +++SSQIV+VAFIFGNT K IFEAIIFLF
Sbjct: 721 ERLGLVVNVIFSIFILVLWIILLGIASRKVILYISSQIVLVAFIFGNTLKNIFEAIIFLF 780

Query: 781 VMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMG 840
           VMHPFDVGDRCEID +QMVVEEMNILTTVFLR+DNLK+I+PNSVLATK+IHN YRSPDMG
Sbjct: 781 VMHPFDVGDRCEIDDIQMVVEEMNILTTVFLRHDNLKIIVPNSVLATKVIHNHYRSPDMG 840

Query: 841 ESVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHR 900
           E +E  VHI TP EKIT MKQRIIS+IE +KEHW P+P+I+ KDID  +K+ +++WLSH 
Sbjct: 841 EFIEICVHIMTPLEKITAMKQRIISFIEADKEHWCPSPIILMKDIDSADKLAMSIWLSHT 900

Query: 901 MNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLP 929
           MNHQD  ERWARRSV+VEEV+K+CQE DI   L+P
Sbjct: 901 MNHQDIRERWARRSVVVEEVLKICQEFDITPTLVP 932

BLAST of Chy1G006540 vs. NCBI nr
Match: XP_004144925.1 (mechanosensitive ion channel protein 6 isoform X1 [Cucumis sativus] >KGN43281.1 hypothetical protein Csa_020565 [Cucumis sativus])

HSP 1 Score: 1810 bits (4688), Expect = 0.0
Identity = 932/955 (97.59%), Postives = 938/955 (98.22%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRD HPVNDSDT
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDGGGSS-AVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNSG 120
           SDRTEVILKIDGGGSS AVSRSLDSA NNGG VWRESRYDFWNNDEIGIGES RMGDNSG
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIIIPLAES 180
           VDRNEGFEFVQTGYGMEDPPTKLIGDFL KQKIEG+TTLDMDLEMEELKPNRI+ PLAES
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180

Query: 181 PLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHERLGSPT 240
           PLSQTSKDLKVSFQ DSTEISSNDQS+RRRNRDSNDLKEESKGGQSP QQPHHERLGSPT
Sbjct: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240

Query: 241 IAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGLIPKSG 300
           I+GVQNESLAEAMRCASNLSFHSELSFQRKSNLLR KTKSRLIDPPAEPDRLSGLIPKSG
Sbjct: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300

Query: 301 QLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360
           QLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNFSALTVLQWVSLIIITAALICTLSVPYLR
Sbjct: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360

Query: 361 EKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420
           EKSLWELDIWKWEVMI ILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN
Sbjct: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420

Query: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480
           CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH
Sbjct: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480

Query: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPPDLKAA 540
           VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGI IPPDLKAA
Sbjct: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540

Query: 541 TFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAWNMKRL 600
           TFASIKSGR IGSGRTHKSFCAKSCKLSRALTKN NDGITIDHLHKLSTKNVSAWNMKRL
Sbjct: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600

Query: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLV 660
           LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYIYLDDLV
Sbjct: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660

Query: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720
           RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM
Sbjct: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720

Query: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780
           VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD
Sbjct: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780

Query: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFL 840
           VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGES+EFL
Sbjct: 781 VGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESIEFL 840

Query: 841 VHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900
           VHIATPAEKIT MK RIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS
Sbjct: 841 VHIATPAEKITAMKHRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDS 900

Query: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 954
           GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 955

BLAST of Chy1G006540 vs. NCBI nr
Match: XP_008448113.1 (PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo])

HSP 1 Score: 1716 bits (4444), Expect = 0.0
Identity = 894/960 (93.12%), Postives = 907/960 (94.48%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60

Query: 61  SDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESE------RM 120
           SDRTEVILKID GGSSAVSRSLDS  NNGGKVWRESRYDFWNNDE GIGES       RM
Sbjct: 61  SDRTEVILKIDDGGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARM 120

Query: 121 GDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIII 180
            D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI G+TTLDMDLEMEELKPNRII 
Sbjct: 121 SD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIP 180

Query: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHER 240
           PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRR RDS+DLKEE KG Q PWQQ HHER
Sbjct: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHER 240

Query: 241 LGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGL 300
           LGSPTI+GVQNESLAEAMRCASNLSFHSELSFQRKSNLLR KTKSRL DPPAEPDRLSGL
Sbjct: 241 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGL 300

Query: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLS 360
           IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNF+ALTVLQW SLI+ITAA ICTLS
Sbjct: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLS 360

Query: 361 VPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVR 420
           VPYLREKSLWELDIWKWEVMI+ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVR
Sbjct: 361 VPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVR 420

Query: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480
           KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL
Sbjct: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+TIPP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPP 540

Query: 541 DLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAW 600
           DLKA TFASIKSGRAI S RTHKSFCAKS K SRALTKNGNDGITIDHLHKLS KNVSAW
Sbjct: 541 DLKA-TFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAW 600

Query: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660
           NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY
Sbjct: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660

Query: 661 LDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVD 720
           LDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAVD
Sbjct: 661 LDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVD 720

Query: 721 KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780
           KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV
Sbjct: 721 KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780

Query: 781 MHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGE 840
           MHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMGE
Sbjct: 781 MHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGE 840

Query: 841 SVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRM 900
           SVEF VHIATPAEKIT MKQRIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHRM
Sbjct: 841 SVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRM 900

Query: 901 NHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 954
           NHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 NHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 951

BLAST of Chy1G006540 vs. NCBI nr
Match: KAA0040324.1 (mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa] >TYK23381.1 mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 1714 bits (4438), Expect = 0.0
Identity = 893/960 (93.02%), Postives = 906/960 (94.38%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGG  SEINHEELPILLNHQ+TDH       H VNDSD 
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGVSSEINHEELPILLNHQSTDH-------HLVNDSDP 60

Query: 61  SDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESE------RM 120
           SDRTEVILKID  GSSAVSRSLDS  NNGGKVWRESRYDFWNNDE GIGES       RM
Sbjct: 61  SDRTEVILKIDDSGSSAVSRSLDSVANNGGKVWRESRYDFWNNDETGIGESASRVRGARM 120

Query: 121 GDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIII 180
            D SG D NEGF+FVQTGYGMEDPPTKLIGDFL KQKI G+TTLDMDLEMEELKPNRII 
Sbjct: 121 SD-SGDDGNEGFQFVQTGYGMEDPPTKLIGDFLHKQKIRGETTLDMDLEMEELKPNRIIP 180

Query: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHER 240
           PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRR RDS+DLKEE KG Q PWQQ HHER
Sbjct: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRYRDSHDLKEEFKGEQPPWQQSHHER 240

Query: 241 LGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGL 300
           LGSPTI+GVQNESLAEAMRCASNLSFHSELSFQRKSNLLR KTKSRL DPPAEPDRLSGL
Sbjct: 241 LGSPTISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLTDPPAEPDRLSGL 300

Query: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLS 360
           IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNF+ALTVLQW SLI+ITAA ICTLS
Sbjct: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFTALTVLQWFSLILITAAFICTLS 360

Query: 361 VPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVR 420
           VPYLREKSLWELDIWKWEVMI+ILICGRLVSGWGIRI VFFIERNFLLRKRVLYFVYGVR
Sbjct: 361 VPYLREKSLWELDIWKWEVMILILICGRLVSGWGIRIAVFFIERNFLLRKRVLYFVYGVR 420

Query: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480
           KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL
Sbjct: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAG+TIPP
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGLTIPP 540

Query: 541 DLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAW 600
           DLKA TFASIKSGRAI S RTHKSFCAKS K SRALTKNGNDGITIDHLHKLS KNVSAW
Sbjct: 541 DLKA-TFASIKSGRAISSERTHKSFCAKSSKFSRALTKNGNDGITIDHLHKLSPKNVSAW 600

Query: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660
           NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY
Sbjct: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660

Query: 661 LDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVD 720
           LDDL+RFMREDEVLKT SLFEGAAEN+RISKS LKNWVVN FRERRALALTLNDTKTAVD
Sbjct: 661 LDDLMRFMREDEVLKTTSLFEGAAENRRISKSVLKNWVVNVFRERRALALTLNDTKTAVD 720

Query: 721 KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780
           KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV
Sbjct: 721 KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780

Query: 781 MHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGE 840
           MHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSPDMGE
Sbjct: 781 MHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPDMGE 840

Query: 841 SVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRM 900
           SVEF VHIATPAEKIT MKQRIISYIEGNKEHW PAPMIVFKDIDGLNK+KLAVWLSHRM
Sbjct: 841 SVEFFVHIATPAEKITAMKQRIISYIEGNKEHWCPAPMIVFKDIDGLNKLKLAVWLSHRM 900

Query: 901 NHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 954
           NHQDS ERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS
Sbjct: 901 NHQDSAERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 951

BLAST of Chy1G006540 vs. NCBI nr
Match: XP_038887578.1 (mechanosensitive ion channel protein 6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1682 bits (4357), Expect = 0.0
Identity = 866/960 (90.21%), Postives = 902/960 (93.96%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNH+  DH R LRD HPVNDSD+
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHEPADHRRRLRDRHPVNDSDS 60

Query: 61  SDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESE------RM 120
           SD TEVILKID GGSS VSRSLDS  +NGGKVWRESRY FWNN+ IGIGES       RM
Sbjct: 61  SDGTEVILKIDDGGSSTVSRSLDSVGSNGGKVWRESRYGFWNNNAIGIGESASRVSGARM 120

Query: 121 GDNSGVDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIII 180
            D SGVDRNEGFEFVQ GYGMEDPPTKLIG+FL KQKI G+TTLDMDLEMEELKP+R + 
Sbjct: 121 SD-SGVDRNEGFEFVQPGYGMEDPPTKLIGEFLHKQKIRGETTLDMDLEMEELKPDRSMT 180

Query: 181 PLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHER 240
           PL ESPL+QTSKDLKVSFQQDSTEISSNDQS+RRR RDS DL EE KGGQ PWQQ HHER
Sbjct: 181 PLPESPLNQTSKDLKVSFQQDSTEISSNDQSVRRRCRDSRDLPEEFKGGQPPWQQSHHER 240

Query: 241 LGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGL 300
           LGSPTI+GVQNES AEAMR ASNLSFHSELSFQRKSNLLR KTKSRLID PAEPDRLSGL
Sbjct: 241 LGSPTISGVQNESSAEAMRSASNLSFHSELSFQRKSNLLRAKTKSRLIDSPAEPDRLSGL 300

Query: 301 IPKSGQLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLS 360
           IPKSGQLRSGFLGKI+DDDDDPFLE+DLPDDFKRGNF+ALTVLQW+SLI+ITAAL+CTLS
Sbjct: 301 IPKSGQLRSGFLGKIDDDDDDPFLEEDLPDDFKRGNFNALTVLQWISLILITAALVCTLS 360

Query: 361 VPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVR 420
           +PYLREKSLWELDIWKWEVMI+ILICGRLVSGWGI+I VFFIERNFLLRK+VLYFVYGVR
Sbjct: 361 IPYLREKSLWELDIWKWEVMILILICGRLVSGWGIKIVVFFIERNFLLRKKVLYFVYGVR 420

Query: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVL 480
           KPVQNCLWLGLVLIAWHLLFNKRVEKQTN SILNYV RVLVCLLIST +WLVKTLMVKVL
Sbjct: 421 KPVQNCLWLGLVLIAWHLLFNKRVEKQTNASILNYVRRVLVCLLISTFLWLVKTLMVKVL 480

Query: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPP 540
           ASSFHVSTYFDRIQESLFNQYVIETLSGPPL+EI KNEEEEER+ADEVQKLQNAGI IP 
Sbjct: 481 ASSFHVSTYFDRIQESLFNQYVIETLSGPPLIEILKNEEEEERVADEVQKLQNAGINIPS 540

Query: 541 DLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAW 600
           DLKAATF+S+KSGR IGSGRTHKSFCAKS KLSRALTKNGNDGITIDHLHKLS KNVSAW
Sbjct: 541 DLKAATFSSVKSGRVIGSGRTHKSFCAKSSKLSRALTKNGNDGITIDHLHKLSPKNVSAW 600

Query: 601 NMKRLLNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660
           NMKRLLNIVRYGSISTLDEQI+GP  DDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY
Sbjct: 601 NMKRLLNIVRYGSISTLDEQIQGPGRDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIY 660

Query: 661 LDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVD 720
           LDDL+RFMREDEV KTMSLFEGAAEN+RISKSALKNWVVNAFRERRALALTLNDTKTAVD
Sbjct: 661 LDDLMRFMREDEVFKTMSLFEGAAENRRISKSALKNWVVNAFRERRALALTLNDTKTAVD 720

Query: 721 KLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780
           KLHHMVNVIF ILILILWLIVLGIASSKFF+FLSSQIVVVAFIFGNTCKTIFEAIIFLFV
Sbjct: 721 KLHHMVNVIFSILILILWLIVLGIASSKFFVFLSSQIVVVAFIFGNTCKTIFEAIIFLFV 780

Query: 781 MHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGE 840
           MHPFDVGDRCEIDG QMVVEEMNILTTVFLRYDNLK+IIPNSVLATKLIHNFYRSP+MGE
Sbjct: 781 MHPFDVGDRCEIDGTQMVVEEMNILTTVFLRYDNLKIIIPNSVLATKLIHNFYRSPEMGE 840

Query: 841 SVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRM 900
           SVEF VHIATPAEKIT MKQRIISYIEGNKEHW P+PM+VF DID LNK+KLAVWLSHRM
Sbjct: 841 SVEFYVHIATPAEKITAMKQRIISYIEGNKEHWCPSPMVVFMDIDDLNKLKLAVWLSHRM 900

Query: 901 NHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSPAS 954
           NHQD+GERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTS AS
Sbjct: 901 NHQDAGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRSLPSSAPSIGFPSNWTSSAS 959

BLAST of Chy1G006540 vs. NCBI nr
Match: XP_031744328.1 (mechanosensitive ion channel protein 6 isoform X2 [Cucumis sativus])

HSP 1 Score: 1489 bits (3855), Expect = 0.0
Identity = 771/791 (97.47%), Postives = 776/791 (98.10%), Query Frame = 0

Query: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDPHPVNDSDT 60
           MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRD HPVNDSDT
Sbjct: 1   MDTLKKSFKGNVSFKHTRKISAGGAGSEINHEELPILLNHQTTDHSRCLRDRHPVNDSDT 60

Query: 61  SDRTEVILKIDGGGSS-AVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNSG 120
           SDRTEVILKIDGGGSS AVSRSLDSA NNGG VWRESRYDFWNNDEIGIGES RMGDNSG
Sbjct: 61  SDRTEVILKIDGGGSSSAVSRSLDSAGNNGGNVWRESRYDFWNNDEIGIGESARMGDNSG 120

Query: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLEMEELKPNRIIIPLAES 180
           VDRNEGFEFVQTGYGMEDPPTKLIGDFL KQKIEG+TTLDMDLEMEELKPNRI+ PLAES
Sbjct: 121 VDRNEGFEFVQTGYGMEDPPTKLIGDFLCKQKIEGETTLDMDLEMEELKPNRIVPPLAES 180

Query: 181 PLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHERLGSPT 240
           PLSQTSKDLKVSFQ DSTEISSNDQS+RRRNRDSNDLKEESKGGQSP QQPHHERLGSPT
Sbjct: 181 PLSQTSKDLKVSFQHDSTEISSNDQSIRRRNRDSNDLKEESKGGQSPRQQPHHERLGSPT 240

Query: 241 IAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAEPDRLSGLIPKSG 300
           I+GVQNESLAEAMRCASNLSFHSELSFQRKSNLLR KTKSRLIDPPAEPDRLSGLIPKSG
Sbjct: 241 ISGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRAKTKSRLIDPPAEPDRLSGLIPKSG 300

Query: 301 QLRSGFLGKIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360
           QLRSGFLGKIEDDDDDPFLEDDLPD+FKRGNFSALTVLQWVSLIIITAALICTLSVPYLR
Sbjct: 301 QLRSGFLGKIEDDDDDPFLEDDLPDEFKRGNFSALTVLQWVSLIIITAALICTLSVPYLR 360

Query: 361 EKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420
           EKSLWELDIWKWEVMI ILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN
Sbjct: 361 EKSLWELDIWKWEVMIFILICGRLVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQN 420

Query: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480
           CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH
Sbjct: 421 CLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFH 480

Query: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGITIPPDLKAA 540
           VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGI IPPDLKAA
Sbjct: 481 VSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIADEVQKLQNAGIIIPPDLKAA 540

Query: 541 TFASIKSGRAIGSGRTHKSFCAKSCKLSRALTKNGNDGITIDHLHKLSTKNVSAWNMKRL 600
           TFASIKSGR IGSGRTHKSFCAKSCKLSRALTKN NDGITIDHLHKLSTKNVSAWNMKRL
Sbjct: 541 TFASIKSGREIGSGRTHKSFCAKSCKLSRALTKNRNDGITIDHLHKLSTKNVSAWNMKRL 600

Query: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLV 660
           LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVA RGYKYIYLDDLV
Sbjct: 601 LNIVRYGSISTLDEQIRGPCLDDESTTEIKSEREAKAAAKKIFQNVAHRGYKYIYLDDLV 660

Query: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720
           RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM
Sbjct: 661 RFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHM 720

Query: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780
           VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD
Sbjct: 721 VNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFD 780

Query: 781 VGDRCEIDGMQ 790
           VGDRCEIDGMQ
Sbjct: 781 VGDRCEIDGMQ 791

BLAST of Chy1G006540 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 951.0 bits (2457), Expect = 7.1e-277
Identity = 517/931 (55.53%), Postives = 656/931 (70.46%), Query Frame = 0

Query: 59  DTSDRTEVILKIDGGGSSAVSRSLDSADNNG------GKVWRESRYDFWNNDEIGIG--- 118
           D +DR EVI+KIDG          ++ +NNG      GK+WR+  YDFW + E  +    
Sbjct: 4   DAADRREVIVKIDG----------ENGNNNGVSGETVGKIWRDGSYDFWTDGEGNLNKGH 63

Query: 119 -----ESERMGDNSG-VDRNEGFEFVQTGYGMEDPPTKLIGDFLRKQKIEGQTTLDMDLE 178
                +S+R    +G   ++EGFEF +     EDPPTKLIG FL KQ+  G+  LDMDL 
Sbjct: 64  NAAAVDSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLG 123

Query: 179 MEELKPNRIIIPLAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGG 238
           M+EL+ +R + P++ESP   T +D                  + RR+  SN         
Sbjct: 124 MDELQ-SRGLTPVSESPRVSTKRD-----------------PVGRRDSRSN--------- 183

Query: 239 QSPWQQPHHERLGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSN-LLRTKTKSRLI 298
                                N    E ++C+ N         QR S+ LL+ +T+SRL 
Sbjct: 184 -------------------TNNNDDGEVVKCSGN-----NAPIQRSSSTLLKMRTRSRLS 243

Query: 299 DPPA------EPDRLSGLIPKSGQLRSGFLGKI-----EDDDDDPFLEDDLPDDFKRGNF 358
           DPP         D  SG IPKSGQ++SGF GK      E+++DDPF  +DLP+++++   
Sbjct: 244 DPPTPQLPPQTADMKSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKL 303

Query: 359 SALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRI 418
           S   VL+W+SLI+I A  +CTL++P LR+K LWEL +WKWE M+++LICGRLVS W ++I
Sbjct: 304 SLWIVLEWLSLILIIAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKI 363

Query: 419 GVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVS 478
            VFFIERNFLLRKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+++V K  NT  L  V+
Sbjct: 364 VVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVT 423

Query: 479 RVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKN 538
           ++ VCLL+  L+WLVKTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+KN
Sbjct: 424 KIFVCLLVGFLLWLVKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKN 483

Query: 539 EEEEERIADEVQKLQNAGITIPPDLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSRALT 598
           EEEEERI+ EV+K QN G              I+SG      +T      KS  LS  L+
Sbjct: 484 EEEEERISVEVKKFQNPG-----------GVEIQSGAQKSPMKT-----GKSPFLSHVLS 543

Query: 599 KNG-----NDGITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT 658
             G     N GITID LHKL+ KNVSAW MKRL+NI+R GS++TLDEQ++ P LDD+   
Sbjct: 544 NGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDKGN 603

Query: 659 EIKSEREAKAAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKS 718
           +I+SE EAK AA+KIF NVA+ G K+IY +D++RF+ +DE LKT+SLFEGA+E  RISKS
Sbjct: 604 QIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKS 663

Query: 719 ALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIF 778
           +LKNWVVNAFRERRALALTLNDTKTAV++LH MVN++ GI+IL++WLI+LGI S+KF + 
Sbjct: 664 SLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVV 723

Query: 779 LSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRY 838
           +SSQ+VVVAFIFGN CK +FE+II+LFV+HPFDVGDRCEIDG+QMVVEEMNILTTVFLR+
Sbjct: 724 MSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRF 783

Query: 839 DNLKVIIPNSVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEH 898
           DN KV+ PNS+L TK I N+YRSPDMG+ +EF +HI TPAEKI ++KQRI SYIEG K+H
Sbjct: 784 DNQKVVYPNSLLWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDH 843

Query: 899 WYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 955
           WYPAPMIVFKD++ LN V++AVW +HRMNHQD GE+WARRS LVEE+ K+C+ELDI+YRL
Sbjct: 844 WYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRL 853

BLAST of Chy1G006540 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 899.4 bits (2323), Expect = 2.4e-261
Identity = 487/926 (52.59%), Postives = 653/926 (70.52%), Query Frame = 0

Query: 59  DTSDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNS 118
           D++DR + I+ I+G  S AV  +  S++  GG +W+ES YDFW+ ++   G++++ GD+ 
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGEK---GKNDKKGDDE 64

Query: 119 GVDRNEGFEFVQTG------YGMEDPPTKLIGDFLRKQKIEG-QTTLDMDLEMEELKPNR 178
             D    F F Q G        + DPP+KLIG FL KQ+  G + +LD++L M EL+ N 
Sbjct: 65  DED-GGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN- 124

Query: 179 IIIPLAESPLSQTSKDLKVSFQQDSTEISSN---DQSMRRRNRDSNDLKEESKGGQSPWQ 238
              P   +  S T +    +  + S+ + +    D   RR+NR S               
Sbjct: 125 --TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTS--------------- 184

Query: 239 QPHHERLGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPA-- 298
                 LG  +    +N   AE ++C S           +K  L R KTKSRL DPP   
Sbjct: 185 ------LGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPT 244

Query: 299 -----EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLPDDFKRG 358
                + +  SG   +SG  +SGFLGK             E++++DPFL++DLP++FKR 
Sbjct: 245 HPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRD 304

Query: 359 NFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGI 418
             S    L+W+SL++I  +L+C+L++  L+ K+ W+LD+WKWEV +++LICGRLVS W +
Sbjct: 305 KLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIV 364

Query: 419 RIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNY 478
           RI VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T ++ L Y
Sbjct: 365 RIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRY 424

Query: 479 VSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIR 538
           V+RVLVCLL++ +IWLVKT++VKVLASSFH+STYFDRIQESLF QYVIETLSGPPL+EI+
Sbjct: 425 VTRVLVCLLVALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQ 484

Query: 539 KNEEEEERIADEVQKLQN-AGITIPPDLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSR 598
           + EEEE+++A++V+ L+  AG  +PP LKA   + +K G++ G              L+R
Sbjct: 485 RMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPG--------------LNR 544

Query: 599 ALTKNGND--GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT 658
             +K G D  GI ID L +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    +DE  T
Sbjct: 545 IGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDAT 604

Query: 659 EIKSEREAKAAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKS 718
            I+SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+ +ISKS
Sbjct: 605 HIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKS 664

Query: 719 ALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIF 778
            LKNWVV AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++F + 
Sbjct: 665 CLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLV 724

Query: 779 LSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRY 838
           LSSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILTTVFLRY
Sbjct: 725 LSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRY 784

Query: 839 DNLKVIIPNSVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEH 898
           DN K+I PNSVL TK I N+YRSPDMG++VEF VHIATP EKIT +KQRI+SY++  K++
Sbjct: 785 DNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDY 844

Query: 899 WYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 950
           WYPAPMIVF  +D LN VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+ELDI+YRL
Sbjct: 845 WYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRL 875

BLAST of Chy1G006540 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 849.7 bits (2194), Expect = 2.2e-246
Identity = 462/921 (50.16%), Postives = 643/921 (69.82%), Query Frame = 0

Query: 60  TSDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNSG 119
           T  R + +++IDG          D+ D+   K WRES  +FW+ND+     S+  G   G
Sbjct: 6   TDQRRDFVVRIDGE---------DNGDSE--KFWRESSINFWHNDK----SSKPPG---G 65

Query: 120 VDRNEGFEFVQTGYGME---DPPTKLIGDFLRKQKIEG-QTTLDMDLEMEELKPNRIIIP 179
            + +  F+F++         DPP+KLI  FL KQK  G + +LDM+  M EL+ N  + P
Sbjct: 66  EEDDGSFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQKN-TVPP 125

Query: 180 LAESPLSQTSKDLKVSFQQDSTEISSNDQSMRRRNRDSNDLKEESKGGQSPWQQPHHERL 239
           L+ + +S ++  +       S    + D   RR+NR +  L    K G S   +    R+
Sbjct: 126 LSSTAVSGSASPVTAPVTA-SYRNGTGDAIRRRQNRVT--LSPSVKDGDS--SEDEENRV 185

Query: 240 GSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPAE--PDRLSG 299
                        +E ++C SN       S  R   L++ KT+SRL+DPP    PD +SG
Sbjct: 186 DG-----------SEVVKCTSN------RSTMRTKTLMKMKTRSRLMDPPTPTYPDMVSG 245

Query: 300 LIPKSGQLRSGFLG-------------KIEDDDDDPFLEDDLPDDFKRGNFSALTVLQWV 359
             P+SG L  GF G             K  ++++DPF E+DLP+  ++       +++W+
Sbjct: 246 RTPRSGNLNPGFSGRNTKPGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWI 305

Query: 360 SLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGIRIGVFFIERNF 419
            LI+I A+LIC+L +PYLR K+LW+L +WKWEVM+++LICGRLVS W +++ V+F+E NF
Sbjct: 306 FLILIIASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNF 365

Query: 420 LLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNYVSRVLVCLLIS 479
           L RK+VLYFVYG+RKPVQNCLWLGLVLIAWH LF+K+VE++  +++L YV++VL+CLL++
Sbjct: 366 LWRKKVLYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVA 425

Query: 480 TLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIRKNEEEEERIAD 539
            +IWL+KTL+VKVLASSFH+STYFDRIQESLF QYVIETLSGPP +EI     EEE++A+
Sbjct: 426 VIIWLIKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---HIEEEKVAN 485

Query: 540 EVQKLQNAGITIPPDLKAATFASIKSGRAIGSGRTHK--SFCAKSCKLSRALTK--NGND 599
           +V+  +  G  + P        S      +GSGR  K  S   KS  LSR+ +K   G +
Sbjct: 486 DVKTFEIVGRKLSP--LGPKAVSSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEE 545

Query: 600 GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCL-DDESTTEIKSEREAK 659
           GI IDHL +++TKNVSAW MK+L+N+++ G++STLDEQI+     +D+  T+I+SE EAK
Sbjct: 546 GIRIDHLQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAK 605

Query: 660 AAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKSALKNWVVNA 719
            AA+KIFQNVA  G +YIY++D +RF+ EDE  + M LFEGA+E  +ISKS LKNWVVNA
Sbjct: 606 LAARKIFQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNA 665

Query: 720 FRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIFLSSQIVVVA 779
           FRERRALALTLNDTKTAV++LH +V+V+  I+ILI+WL++LGIA++KF + +SSQ+++V 
Sbjct: 666 FRERRALALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVV 725

Query: 780 FIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRYDNLKVIIPN 839
           F+FGN+CKTIFEA+IF+FVMHPFDVGDRCEIDG+QM+VEEMNILTTVFLR+DN K++ PN
Sbjct: 726 FVFGNSCKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPN 785

Query: 840 SVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEHWYPAPMIVF 899
           S+L TK I N+YRSPDM +++EF VHIATP EK T ++QRI+SY++  K+HW+P+PMIVF
Sbjct: 786 SLLGTKPIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVF 845

Query: 900 KDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRLLPIDINIRS 955
           +D+ GLN VK+A+W +H+MNHQ+ GER+ RR  L+EE+ ++C+ELDI+YRL P++IN++S
Sbjct: 846 RDMCGLNSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKS 880

BLAST of Chy1G006540 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 829.3 bits (2141), Expect = 3.1e-240
Identity = 461/926 (49.78%), Postives = 620/926 (66.95%), Query Frame = 0

Query: 59  DTSDRTEVILKIDGGGSSAVSRSLDSADNNGGKVWRESRYDFWNNDEIGIGESERMGDNS 118
           D++DR + I+ I+G  S AV  +  S++  GG +W+ES YDFW+ ++   G++++ GD+ 
Sbjct: 5   DSTDRRDFIVNINGQESGAVGATGSSSNAEGGNIWKESSYDFWDGEK---GKNDKKGDDE 64

Query: 119 GVDRNEGFEFVQTG------YGMEDPPTKLIGDFLRKQKIEG-QTTLDMDLEMEELKPNR 178
             D    F F Q G        + DPP+KLIG FL KQ+  G + +LD++L M EL+ N 
Sbjct: 65  DED-GGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN- 124

Query: 179 IIIPLAESPLSQTSKDLKVSFQQDSTEISSN---DQSMRRRNRDSNDLKEESKGGQSPWQ 238
              P   +  S T +    +  + S+ + +    D   RR+NR S               
Sbjct: 125 --TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQNRTS--------------- 184

Query: 239 QPHHERLGSPTIAGVQNESLAEAMRCASNLSFHSELSFQRKSNLLRTKTKSRLIDPPA-- 298
                 LG  +    +N   AE ++C S           +K  L R KTKSRL DPP   
Sbjct: 185 ------LGGSSDEEGRNRDEAEVLKCGS-----------KKPMLSRNKTKSRLQDPPTPT 244

Query: 299 -----EPDRLSGLIPKSGQLRSGFLGKI------------EDDDDDPFLEDDLPDDFKRG 358
                + +  SG   +SG  +SGFLGK             E++++DPFL++DLP++FKR 
Sbjct: 245 HPAIDKTEMKSG--RRSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRD 304

Query: 359 NFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGRLVSGWGI 418
             S    L+W+SL++I  +L+C+L++  L+ K+ W+LD+WKWEV +++LICGRLVS W +
Sbjct: 305 KLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIV 364

Query: 419 RIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQTNTSILNY 478
           RI VF +E+NF  RKRVLYFVYGVRK VQNCLWLGLVL+AWH LF+K+VE++T ++ L  
Sbjct: 365 RIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALR- 424

Query: 479 VSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSGPPLVEIR 538
                                           TYFDRIQESLF QYVIETLSGPPL+EI+
Sbjct: 425 --------------------------------TYFDRIQESLFTQYVIETLSGPPLMEIQ 484

Query: 539 KNEEEEERIADEVQKLQN-AGITIPPDLKAATFASIKSGRAIGSGRTHKSFCAKSCKLSR 598
           + EEEE+++A++V+ L+  AG  +PP LKA   + +K G++ G              L+R
Sbjct: 485 RMEEEEQQVAEDVKSLEKLAGAKLPPALKATVKSFMKVGKSPG--------------LNR 544

Query: 599 ALTKNGND--GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLDDESTT 658
             +K G D  GI ID L +++TKNVSAWNMKRL+NI+  G+ISTLD+ ++    +DE  T
Sbjct: 545 IGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTTQEDEDAT 604

Query: 659 EIKSEREAKAAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQRISKS 718
            I+SE EAK AA+KIF NV   G +YIYL+D +RF+ E+E  + M+LFEGA+E+ +ISKS
Sbjct: 605 HIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKS 664

Query: 719 ALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASSKFFIF 778
            LKNWV  AFRERRALALTLNDTKTAVD+LH ++NV+ GI+I+I+WL++LGIA+++F + 
Sbjct: 665 CLKNWV--AFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLV 724

Query: 779 LSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTTVFLRY 838
           LSSQ+++VAF+FGN+CKTIFEAIIFLFVMHPFDVGDRCEIDG+Q+VVEEMNILTTVFLRY
Sbjct: 725 LSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRY 784

Query: 839 DNLKVIIPNSVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIEGNKEH 898
           DN K+I PNSVL TK I N+YRSPDMG++VEF VHIATP EKIT +KQRI+SY++  K++
Sbjct: 785 DNQKIIYPNSVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDY 840

Query: 899 WYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELDIQYRL 950
           WYPAPMIVF  +D LN VK+AVWL+HRMNHQD GER+ RR +L+EEV K C+ELDI+YRL
Sbjct: 845 WYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRL 840

BLAST of Chy1G006540 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 784.3 bits (2024), Expect = 1.1e-226
Identity = 399/689 (57.91%), Postives = 534/689 (77.50%), Query Frame = 0

Query: 268 RKSNLL-RTKTKSRLIDPPAEPD-RLSGLIPKSGQLRSGFLGKIEDD---DDDPFLEDDL 327
           RK+ L+ R K +SRLIDPP E + + S  I  S QLRSG LG+  DD   +DD   E+D+
Sbjct: 161 RKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRSGLLGRHSDDIEEEDDSSAEEDV 220

Query: 328 PDDFKRGNFSALTVLQWVSLIIITAALICTLSVPYLREKSLWELDIWKWEVMIMILICGR 387
           P ++++    A+T+LQW+SLI +  AL+ +L +   R  +LW L +WKWEV++++LICGR
Sbjct: 221 PVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRNATLWSLHLWKWEVVLLVLICGR 280

Query: 388 LVSGWGIRIGVFFIERNFLLRKRVLYFVYGVRKPVQNCLWLGLVLIAWHLLFNKRVEKQT 447
           LVSG GIRI VFFIERNFLLRKRVLYFVYGV+  VQNCLWLGLVL+AWH LF+K+VEK+T
Sbjct: 281 LVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNCLWLGLVLLAWHFLFDKKVEKET 340

Query: 448 NTSILNYVSRVLVCLLISTLIWLVKTLMVKVLASSFHVSTYFDRIQESLFNQYVIETLSG 507
            + +L  +S++LVC L+ST++WL+KTL+VKVLASSFHVSTYFDRIQE+LF+ Y+IETLSG
Sbjct: 341 QSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHVSTYFDRIQEALFHHYLIETLSG 400

Query: 508 PPLVEIRKNEEEEERIADEVQKLQNAGITIPPDLKAATFASIKSGRAIGSGRTHKSFCAK 567
           PP++E+ + EEEE+R  DE+ K+Q  G  + P+L +A F   KSG  +            
Sbjct: 401 PPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAAFPQEKSGSTM------------ 460

Query: 568 SCKLSRALTKNGND-GITIDHLHKLSTKNVSAWNMKRLLNIVRYGSISTLDEQIRGPCLD 627
           + K S  + K G+D GIT+D LHK++ KNVSAWNMKRL+ IVR  S+STLDEQ      +
Sbjct: 461 NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRLMKIVRNVSLSTLDEQALQNTCE 520

Query: 628 DESTTEIKSEREAKAAAKKIFQNVARRGYKYIYLDDLVRFMREDEVLKTMSLFEGAAENQ 687
           DEST +I+SE+EAKAAA+KIF+NVA+ G K+IYL+DL+RF+R DE +KTM LFEGA   +
Sbjct: 521 DESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDLMRFLRVDEAMKTMCLFEGALVTK 580

Query: 688 RISKSALKNWVVNAFRERRALALTLNDTKTAVDKLHHMVNVIFGILILILWLIVLGIASS 747
           +I+KSALKNW+VNAFRERRALALTLNDTKTAV+KLHHM++ +  I+I+++WLI+L IA+S
Sbjct: 581 KITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHHMISFLTAIVIIVIWLILLEIATS 640

Query: 748 KFFIFLSSQIVVVAFIFGNTCKTIFEAIIFLFVMHPFDVGDRCEIDGMQMVVEEMNILTT 807
           K+ +FL+SQ+V++AF+FGN+ KT+FE+IIFLF++HP+DVGDR  ID ++MVVEEMNILTT
Sbjct: 641 KYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPYDVGDRLLIDTVEMVVEEMNILTT 700

Query: 808 VFLRYDNLKVIIPNSVLATKLIHNFYRSPDMGESVEFLVHIATPAEKITVMKQRIISYIE 867
           VFLR DNLK++ PN +L  K IHN+ RSPDMG+ V   VHI TP EKI  +KQRI SYI+
Sbjct: 701 VFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTCCVHITTPPEKIAAIKQRISSYID 760

Query: 868 GNKEHWYPAPMIVFKDIDGLNKVKLAVWLSHRMNHQDSGERWARRSVLVEEVVKVCQELD 927
              E+WYP   ++ KD++ LN V++A+WL H++NHQ+ GER+ RR++L+EEV+K+  ELD
Sbjct: 761 SKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQNMGERFTRRALLIEEVIKILLELD 820

Query: 928 IQYRLLPIDINIRSLPSSAPSIGFPSNWT 951
           IQYR  P+DIN++++P+   S   P  W+
Sbjct: 821 IQYRFHPLDINVKTMPTVVSS-RVPPAWS 836

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SYM19.9e-27655.53Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 P... [more]
Q9LH743.4e-26052.59Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 P... [more]
F4IME28.4e-25951.12Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 P... [more]
Q9LPG33.1e-24550.16Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 P... [more]
F4IME11.6e-22557.91Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 P... [more]
Match NameE-valueIdentityDescription
A0A0A0K6190.0e+0097.59Mechanosensitive ion channel protein OS=Cucumis sativus OX=3659 GN=Csa_7G017150 ... [more]
A0A1S3BID30.0e+0093.13Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490400 PE=... [more]
A0A5D3DI810.0e+0093.02Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1C2880.0e+0079.79Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111007... [more]
A0A6J1EJZ40.0e+0071.76mechanosensitive ion channel protein 6-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
XP_004144925.10.097.59mechanosensitive ion channel protein 6 isoform X1 [Cucumis sativus] >KGN43281.1 ... [more]
XP_008448113.10.093.13PREDICTED: mechanosensitive ion channel protein 6-like [Cucumis melo][more]
KAA0040324.10.093.02mechanosensitive ion channel protein 6-like [Cucumis melo var. makuwa] >TYK23381... [more]
XP_038887578.10.090.21mechanosensitive ion channel protein 6-like isoform X1 [Benincasa hispida][more]
XP_031744328.10.097.47mechanosensitive ion channel protein 6 isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT1G78610.17.1e-27755.53mechanosensitive channel of small conductance-like 6 [more]
AT3G14810.12.4e-26152.59mechanosensitive channel of small conductance-like 5 [more]
AT1G53470.12.2e-24650.16mechanosensitive channel of small conductance-like 4 [more]
AT3G14810.23.1e-24049.78mechanosensitive channel of small conductance-like 5 [more]
AT2G17000.11.1e-22657.91Mechanosensitive ion channel family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 508..528
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 185..201
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 185..237
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..219
NoneNo IPR availablePANTHERPTHR31618:SF27MECHANOSENSITIVE ION CHANNEL PROTEINcoord: 116..938
coord: 3..106
IPR006685Mechanosensitive ion channel MscSPFAMPF00924MS_channelcoord: 719..923
e-value: 4.7E-20
score: 72.0
IPR023408Mechanosensitive ion channel MscS domain superfamilyGENE3D2.30.30.60coord: 777..826
e-value: 4.9E-12
score: 47.6
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPIRSFPIRSF017209Memb_At2g17000coord: 51..954
e-value: 2.8E-265
score: 880.6
IPR016688Mechanosensitive ion channel MscS-like, plants/fungiPANTHERPTHR31618MECHANOSENSITIVE ION CHANNEL PROTEIN 5coord: 116..938
coord: 3..106
IPR010920LSM domain superfamilySUPERFAMILY50182Sm-like ribonucleoproteinscoord: 767..826

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G006540.1Chy1G006540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006820 anion transport
biological_process GO:0034220 ion transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0008381 mechanosensitive ion channel activity