Homology
BLAST of Chy1G005480 vs. ExPASy Swiss-Prot
Match:
Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)
HSP 1 Score: 957.6 bits (2474), Expect = 8.2e-278
Identity = 474/739 (64.14%), Postives = 572/739 (77.40%), Query Frame = 0
Query: 2 GAHSHGGQKPADV---VANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVA 61
GAHSH G+ D V +H++FL F S E T D IFYSYT+HINGFAA L+ ++A
Sbjct: 37 GAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERAT-DAIFYSYTKHINGFAAHLDHDLA 96
Query: 62 AQLAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDT 121
+++KHP VVSVF N+ KLHTTRSW+F+GLE N + S SIW+KARFGEDTII NLDT
Sbjct: 97 YEISKHPEVVSVFPNKALKLHTTRSWDFLGLE-HNSYVPSSSIWRKARFGEDTIIANLDT 156
Query: 122 GVWAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSF 181
GVW ESKSF D+ GPIP RWKGICQNQ D +FHCNRKLIGARYFNKGYA+ VG LNSSF
Sbjct: 157 GVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSF 216
Query: 182 HSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECF 241
SPRD +GHGSHTLSTA G+FV G S+FG G GTAKGGSPRARVAAYKVCWPP GNEC+
Sbjct: 217 DSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECY 276
Query: 242 DADILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAA 301
DAD+LA FD AIHDG DV+SVSLGG+P FNDSVAIGSFHA K IVV+CSAGNSGPA
Sbjct: 277 DADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPAD 336
Query: 302 GTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRL 361
TV+NVAPWQITVGASTMDR+F S +VLGN K +G+SLS ALP K YP+M + + +
Sbjct: 337 STVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKA 396
Query: 362 ANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGN 421
NASA +AQLCK G+LDP+K KGKILVCLRG N RV+KG L G GM+L N ++GN
Sbjct: 397 KNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGN 456
Query: 422 EILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPN 481
++LADPHVLPA+ + D V YI+ TK P A+ITP+ T LG++PAP MA+FSS GP+
Sbjct: 457 DLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPS 516
Query: 482 TVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLL 541
V P+ILKPDITAPG+SVIAAY+ A PTN++FD RR+ FN++SGTSMSCPH+SGIAGLL
Sbjct: 517 IVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLL 576
Query: 542 KTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIY 601
KT YP WSPAAI+SAIMTTA+ +D+ P+ NA+ A+PF++GAGHV PN A +PGL+Y
Sbjct: 577 KTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVY 636
Query: 602 DIEVNEYLSFLCALGYNKAQISQFSNGPFNCSEP-ISPTNLNYPSITVPELSRS-IAITR 661
D+ + +YL+FLC+LGYN +QIS FS F CS P IS NLNYPSITVP L+ S + ++R
Sbjct: 637 DLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSR 696
Query: 662 RLKNVGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYG 721
+KNVG P Y V++ P G+ V VKP LNFT++GE+ +FKV + + VA K YV+G
Sbjct: 697 TVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVA-KGYVFG 756
Query: 722 DLIWSDGKHHVRSPIVVKV 736
+L+WSD KH VRSPIVVK+
Sbjct: 757 ELVWSDKKHRVRSPIVVKL 772
BLAST of Chy1G005480 vs. ExPASy Swiss-Prot
Match:
F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)
HSP 1 Score: 890.2 bits (2299), Expect = 1.6e-257
Identity = 439/740 (59.32%), Postives = 554/740 (74.86%), Query Frame = 0
Query: 1 MGAHSHGGQKPA---DVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEV 60
+G+H+H Q + D VA+SH FL F+ S E + IFYSY RHINGFAA+L++
Sbjct: 46 LGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN-AKEAIFYSYKRHINGFAAILDENE 105
Query: 61 AAQLAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLD 120
AA++AKHP VVSVF N+GRKLHTT SW FM L K NGV++ S+W KA +GEDTII NLD
Sbjct: 106 AAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAK-NGVVHKSSLWNKAGYGEDTIIANLD 165
Query: 121 TGVWAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVG-PLNS 180
TGVW ESKSFSD+ YG +P RWKG C CNRKLIGARYFNKGY + G P N+
Sbjct: 166 TGVWPESKSFSDEGYGAVPARWKGRCHK----DVPCNRKLIGARYFNKGYLAYTGLPSNA 225
Query: 181 SFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNE 240
S+ + RD +GHGSHTLSTA GNFV GA+VFG+G GTA GGSP+ARVAAYKVCWPP G E
Sbjct: 226 SYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAE 285
Query: 241 CFDADILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGP 300
CFDADILA + AI DGVDVLS S+GGD +D +AIGSFHA+K+G+ V+CSAGNSGP
Sbjct: 286 CFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGP 345
Query: 301 AAGTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADV 360
+GTV+NVAPW ITVGAS+MDR+F + V L N + +G SLS+ LP +K+Y L++AAD
Sbjct: 346 KSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSK-PLPEEKMYSLISAADA 405
Query: 361 RLANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELS 420
+AN + +A LCK G+LDP K KGKILVCLRGDNARVDKG QA AGAAGM+L N++ S
Sbjct: 406 NVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKAS 465
Query: 421 GNEILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVG 480
GNEI++D HVLPAS I++ DG +F+Y++STK P+ YI T L +PAPFMA+FSS G
Sbjct: 466 GNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRG 525
Query: 481 PNTVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAG 540
PNT+TP ILKPDITAPG+++IAA++EA GPT+ + DNRR PFN+ SGTSMSCPH+SG+ G
Sbjct: 526 PNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVG 585
Query: 541 LLKTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGL 600
LLKTL+PHWSPAAI+SAIMTT+ T +N +P+++ S+ A+PF+YG+GHV PN A PGL
Sbjct: 586 LLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGL 645
Query: 601 IYDIEVNEYLSFLCALGYNKAQISQFSNGP-FNCSEPISPTNLNYPSITVPELSRSIAIT 660
+YD+ +YL FLCA+GYN + F+ P + C + + + NYPSITVP L+ SI +T
Sbjct: 646 VYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVT 705
Query: 661 RRLKNVGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVY 720
R+LKNVG P TY R+P G+ V V+PK+L F + GE F+ M ++ V YV+
Sbjct: 706 RKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQ--MTLRPLPVTPSGYVF 765
Query: 721 GDLIWSDGKHHVRSPIVVKV 736
G+L W+D H+VRSPIVV++
Sbjct: 766 GELTWTDSHHYVRSPIVVQL 776
BLAST of Chy1G005480 vs. ExPASy Swiss-Prot
Match:
I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)
HSP 1 Score: 694.5 bits (1791), Expect = 1.3e-198
Identity = 382/760 (50.26%), Postives = 491/760 (64.61%), Query Frame = 0
Query: 1 MGAHSHGGQKPA---DVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEV 60
MGAHSHG + ++ +SH++ L S E+ + I YSY RHINGFAA+LE+E
Sbjct: 36 MGAHSHGPSPTSADLELATDSHYDLLGSIFGSREK-AKEAIIYSYNRHINGFAALLEEEE 95
Query: 61 AAQLAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLD 120
AA +AK+P VVSVFL++ KLHTTRSWEF+GL + S W+K RFGE+TIIGN+D
Sbjct: 96 AADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRR----GQNSAWQKGRFGENTIIGNID 155
Query: 121 TGVWAESKSFSDDEYGPIPHRWK-GICQNQNDPSF---HCNRKLIGARYFNKGYASVVGP 180
TGVW ES+SFSD YG +P +W+ G+CQ P CNRKLIGARY+NK + + G
Sbjct: 156 TGVWPESQSFSDKGYGTVPSKWRGGLCQINKLPGSMKNTCNRKLIGARYYNKAFEAHNGQ 215
Query: 181 LNSSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKG 240
L+ H+ RD GHG+HTLSTAGGNFV GA VF +G GTAKGGSPRARVAAYKVCW
Sbjct: 216 LDPLLHTARDFVGHGTHTLSTAGGNFVPGARVFAVGNGTAKGGSPRARVAAYKVCWSLTD 275
Query: 241 GNECFDADILAGFDFAIHDGVDVLSVSLGGD----PNPLFNDSVAIGSFHAIKHGIVVIC 300
C+ AD+LA D AI DGVDV++VS G +F D ++IG+FHAI I+++
Sbjct: 276 PASCYGADVLAAIDQAIDDGVDVINVSFGVSYVVTAEGIFTDEISIGAFHAISKNILLVA 335
Query: 301 SAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYP 360
SAGN GP GTV NVAPW T+ AST+DR F S + + N + IEG SL + LP + +
Sbjct: 336 SAGNDGPTPGTVANVAPWVFTIAASTLDRDFSSNLTI-NNQLIEGASLFVN-LPPNQAFS 395
Query: 361 LMNAADVRLANASAHEAQLCKAGTLDPMKAKGKILVCLR-GDNARVDKGEQALLAGAAGM 420
L+ + D +LANA+ +AQLC+ GTLD K GKI++C R G V +G +AL AGA GM
Sbjct: 396 LILSTDAKLANATFRDAQLCRRGTLDRTKVNGKIVLCTREGKIKSVAEGLEALTAGARGM 455
Query: 421 ILANNELSGNEILADPHVL-----PASHINFTDGSLVFAYINSTKYP-----EAYITPAT 480
IL N +G + A+PHV P + I P ++ A
Sbjct: 456 ILNNQMQNGKTLSAEPHVFSTVNTPPRRAKSRPHGVKTTAIGDEDDPLKTGDTIKMSRAR 515
Query: 481 TQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRR-IP 540
T G +PAP MA+FSS GPN + P ILKPD+TAPG++++AAYSE ++ DNRR
Sbjct: 516 TLFGRKPAPVMASFSSRGPNKIQPSILKPDVTAPGVNILAAYSEFASASSLLVDNRRGFK 575
Query: 541 FNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNA-SYSVA 600
FN + GTSMSCPH SGIAGLLKT +P WSPAAIKSAIMTTA+TLDN P+ +A ++A
Sbjct: 576 FNVLQGTSMSCPHASGIAGLLKTRHPSWSPAAIKSAIMTTATTLDNTNRPIQDAFDKTLA 635
Query: 601 SPFNYGAGHVHPNGATDPGLIYDIEVNEYLSFLCALGYNKAQISQFS-NGPFNCSEPISP 660
F YG+GHV P+ A +PGL+YD+ + +YL+FLCA GY++ IS + N F CS S
Sbjct: 636 DAFAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCASGYDQQLISALNFNRTFICSGSHSV 695
Query: 661 TNLNYPSITVPELS-RSIAITRRLKNVGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEE 720
+LNYPSIT+P L + + I R + NVG P TY V R P G S+ V P L FT++GE
Sbjct: 696 NDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGER 755
Query: 721 LSFKVFMKVKEHKVAKKNYVYGDLIWSDGKHHVRSPIVVK 735
+FKV ++ ++ Y +GDL W+DGKH VRSPI VK
Sbjct: 756 KTFKVIVQASS-AATRRKYEFGDLRWTDGKHIVRSPITVK 787
BLAST of Chy1G005480 vs. ExPASy Swiss-Prot
Match:
O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)
HSP 1 Score: 614.0 bits (1582), Expect = 2.2e-174
Identity = 342/741 (46.15%), Postives = 472/741 (63.70%), Query Frame = 0
Query: 4 HSHGGQKPA--DVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQL 63
H Q P+ D+ +N + L+ S E + Y+Y I+GF+ L E A L
Sbjct: 35 HMAKSQMPSSFDLHSNWYDSSLRSISDSAE------LLYTYENAIHGFSTRLTQEEADSL 94
Query: 64 AKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGVW 123
P V+SV +LHTTR+ F+GL++ ++ ++ +A D ++G LDTGVW
Sbjct: 95 MTQPGVISVLPEHRYELHTTRTPLFLGLDE-----HTADLFPEAGSYSDVVVGVLDTGVW 154
Query: 124 AESKSFSDDEYGPIPHRWKGICQ-NQNDPSFHCNRKLIGARYFNKGYASVVGPLNSS--F 183
ESKS+SD+ +GPIP WKG C+ N + CNRKLIGAR+F +GY S +GP++ S
Sbjct: 155 PESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKES 214
Query: 184 HSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECF 243
SPRD +GHG+HT STA G+ V GAS+ G GTA+G +PRARVA YKVCW GG CF
Sbjct: 215 RSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW--LGG--CF 274
Query: 244 DADILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAA 303
+DILA D AI D V+VLS+SLGG + + D VAIG+F A++ GI+V CSAGN+GP++
Sbjct: 275 SSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSS 334
Query: 304 GTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQ-DALPSKKLYPLMNAADVR 363
+++NVAPW TVGA T+DR FP+L +LGN K G SL + +ALP KL P + A +
Sbjct: 335 SSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLPFIYAGNA- 394
Query: 364 LANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSG 423
++A LC GTL P K KGKI++C RG NARV KG+ AG GMILAN +G
Sbjct: 395 ---SNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANG 454
Query: 424 NEILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGP 483
E++AD H+LPA+ + G ++ Y+ + P A I+ T +G++P+P +AAFSS GP
Sbjct: 455 EELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGP 514
Query: 484 NTVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGL 543
N++TP ILKPD+ APG++++AA++ A GPT D+RR+ FN +SGTSMSCPHVSG+A L
Sbjct: 515 NSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAAL 574
Query: 544 LKTLYPHWSPAAIKSAIMTTASTLDNNFEPLLN-ASYSVASPFNYGAGHVHPNGATDPGL 603
LK+++P WSPAAI+SA+MTTA + +PLL+ A+ ++PF++GAGHV P AT+PGL
Sbjct: 575 LKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGL 634
Query: 604 IYDIEVNEYLSFLCALGYNKAQISQFSNGPFNC--SEPISPTNLNYPSITV-PELSRSIA 663
IYD+ +YL FLCAL Y QI S + C S+ S +LNYPS V + +
Sbjct: 635 IYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYK 694
Query: 664 ITRRLKNVGSPGTYEVEI-RKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKN 723
TR + +VG GTY V++ + G+ + V+P LNF E+ S+ V V K + N
Sbjct: 695 YTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN 754
Query: 724 YVYGDLIWSDGKHHVRSPIVV 734
+G + WSDGKH V SP+ +
Sbjct: 755 -SFGSIEWSDGKHVVGSPVAI 754
BLAST of Chy1G005480 vs. ExPASy Swiss-Prot
Match:
Q9LUM3 (Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 SV=1)
HSP 1 Score: 606.7 bits (1563), Expect = 3.5e-172
Identity = 335/718 (46.66%), Postives = 452/718 (62.95%), Query Frame = 0
Query: 38 IFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVIN 97
I ++Y +GF+A L + A+QL HP V+SV + R LHTTRS EF+GL +
Sbjct: 62 IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS----TD 121
Query: 98 SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC-QNQNDPSFHCNRK 157
+ +++ FG D +IG +DTGVW E SF D GP+P +WKG C +Q+ P CNRK
Sbjct: 122 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 181
Query: 158 LIGARYFNKGYASVVGPLN--SSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAK 217
L+GAR+F GY + G +N + F SPRD +GHG+HT S + G +V AS G G A
Sbjct: 182 LVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAA 241
Query: 218 GGSPRARVAAYKVCWPPKGGNECFDADILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVA 277
G +P+AR+AAYKVCW + C+D+DILA FD A+ DGVDV+S+S+GG P + D++A
Sbjct: 242 GMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIA 301
Query: 278 IGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEG 337
IG+F AI GI V SAGN GP A TVTNVAPW TVGA T+DR FP+ V LGN K I G
Sbjct: 302 IGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISG 361
Query: 338 ESL-SQDALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNAR 397
S+ L ++YPL+ L + + LC G+LDP KGKI++C RG N+R
Sbjct: 362 VSVYGGPGLDPGRMYPLVYGGS--LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSR 421
Query: 398 VDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSLVFAYIN------ST 457
KGE G GMI+AN G ++AD HVLPA+ + + G + YI+ S+
Sbjct: 422 ATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSS 481
Query: 458 KYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYSEAEGPT 517
K+P A I T+LGIRPAP +A+FS+ GPN TPEILKPD+ APGL+++AA+ + GP+
Sbjct: 482 KHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPS 541
Query: 518 NQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASTLDNNFEP 577
DNRR FN +SGTSM+CPHVSG+A LLK +P WSPAAI+SA++TTA T+DN+ EP
Sbjct: 542 GVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEP 601
Query: 578 LLNASY-SVASPFNYGAGHVHPNGATDPGLIYDIEVNEYLSFLCALGYNKAQISQFSNGP 637
+++ S + +S +YG+GHVHP A DPGL+YDI +Y++FLC Y + I +
Sbjct: 602 MMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQ 661
Query: 638 FNCS---EPISPTNLNYPSITV-----PELSRSIAITRRLKNVG-SPGTYEVEIRKPAGI 697
+C NLNYPS +V E S R + NVG S YE++IR P G
Sbjct: 662 ADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGT 721
Query: 698 SVWVKPKKLNFTRLGEELSFKVFMKVKEHKVA--KKNYVYGDLIWSDGKHHVRSPIVV 734
+V V+P+KL+F R+G++LSF V +K E K++ N G ++WSDGK +V SP+VV
Sbjct: 722 TVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVV 769
BLAST of Chy1G005480 vs. ExPASy TrEMBL
Match:
A0A0A0K313 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G030520 PE=3 SV=1)
HSP 1 Score: 1454.5 bits (3764), Expect = 0.0e+00
Identity = 713/736 (96.88%), Postives = 722/736 (98.10%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFT DVIFYSYTRHINGFAAMLEDEVA Q
Sbjct: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQ 60
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHP+VVSVFLNRGRKLHTTRSWEFMGLE +NGVINSESIWKKARFGEDTIIGNLDTGV
Sbjct: 61 LAKHPKVVSVFLNRGRKLHTTRSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGV 120
Query: 121 WAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
WAESKSFSDDEYGPIPHRWKGICQNQ DPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS
Sbjct: 121 WAESKSFSDDEYGPIPHRWKGICQNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
Query: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFDA 240
PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPK GNECFDA
Sbjct: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 240
Query: 241 DILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
DILA FDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT
Sbjct: 241 DILAAFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
Query: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 360
VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN
Sbjct: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 360
Query: 361 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 420
AS HEAQLCKAGTL+PMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI
Sbjct: 361 ASVHEAQLCKAGTLNPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 420
Query: 421 LADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 480
LADPHVLPASHINFTDGS VFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV
Sbjct: 421 LADPHVLPASHINFTDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 480
Query: 481 TPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 540
TPEILKPDITAPGLSVIAAY+EAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT
Sbjct: 481 TPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 540
Query: 541 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIYDI 600
LYPHWSPAAIKSAIMTTAS LDNNFEPLLNASYSVASPFNYGAGHVHPNGA DPGL+YDI
Sbjct: 541 LYPHWSPAAIKSAIMTTASILDNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDI 600
Query: 601 EVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRLKN 660
EVNEYLSFLCALGYNKAQISQFSNGPFNCS+PISPTNLNYPSITVP+LSRSI ITRRLKN
Sbjct: 601 EVNEYLSFLCALGYNKAQISQFSNGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKN 660
Query: 661 VGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYGDLIW 720
VGSPGTY+ EIRKPAGISVWVKPKKL+FTRLGEELSFKV MKVKE KVAKKNYVYGDLIW
Sbjct: 661 VGSPGTYKAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIW 720
Query: 721 SDGKHHVRSPIVVKVV 737
SDGKHHVRSPIVVKVV
Sbjct: 721 SDGKHHVRSPIVVKVV 736
BLAST of Chy1G005480 vs. ExPASy TrEMBL
Match:
A0A5D3BUG6 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84663G00160 PE=3 SV=1)
HSP 1 Score: 1439.5 bits (3725), Expect = 0.0e+00
Identity = 704/736 (95.65%), Postives = 722/736 (98.10%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGG+KPADVVA+SHH+FLQPFLKSGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38 MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHPRVVSVFLNRGRKLHTTRSWEFMGLE ENGVI SESIWKKARFGEDTIIGNLDTGV
Sbjct: 98 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGV 157
Query: 121 WAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
WAESKSFSDDE GPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS
Sbjct: 158 WAESKSFSDDEMGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 217
Query: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFDA 240
PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPK GNECFDA
Sbjct: 218 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 277
Query: 241 DILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
DILA FD AIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT
Sbjct: 278 DILAAFDLAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 337
Query: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 360
VTNVAPWQITVGASTMDRKFPSLVVLGN+KQIEGESLSQ+ALPSKKLYPLMNAADVRLAN
Sbjct: 338 VTNVAPWQITVGASTMDRKFPSLVVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLAN 397
Query: 361 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 420
ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI
Sbjct: 398 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 457
Query: 421 LADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 480
LADPHVLPA HIN+TDGS VFAYINSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTV
Sbjct: 458 LADPHVLPALHINYTDGSAVFAYINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 517
Query: 481 TPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 540
TPEILKPDITAPGLSVIAAY+EAEGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+T
Sbjct: 518 TPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRT 577
Query: 541 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIYDI 600
LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASY VA+PFNYGAGHVHPNGATDPGL+YDI
Sbjct: 578 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDI 637
Query: 601 EVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRLKN 660
+VNEYLSFLCALGY+KAQISQFS+GPFNCSEPIS TNLNYPSITVP+LSRSI ITRRLKN
Sbjct: 638 DVNEYLSFLCALGYDKAQISQFSDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKN 697
Query: 661 VGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYGDLIW 720
VGSPGTYE +IRKPAGISVWVKPKKLNFT+LGEELSFKVFMKVK HKVAKKNYVYGDLIW
Sbjct: 698 VGSPGTYEAKIRKPAGISVWVKPKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIW 757
Query: 721 SDGKHHVRSPIVVKVV 737
SDGKHHVRSPIVVKVV
Sbjct: 758 SDGKHHVRSPIVVKVV 773
BLAST of Chy1G005480 vs. ExPASy TrEMBL
Match:
A0A1S3CG03 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103500491 PE=3 SV=1)
HSP 1 Score: 1439.5 bits (3725), Expect = 0.0e+00
Identity = 704/736 (95.65%), Postives = 722/736 (98.10%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGG+KPADVVA+SHH+FLQPFLKSGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38 MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHPRVVSVFLNRGRKLHTTRSWEFMGLE ENGVI SESIWKKARFGEDTIIGNLDTGV
Sbjct: 98 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGV 157
Query: 121 WAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
WAESKSFSDDE GPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS
Sbjct: 158 WAESKSFSDDEMGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 217
Query: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFDA 240
PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPK GNECFDA
Sbjct: 218 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 277
Query: 241 DILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
DILA FD AIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT
Sbjct: 278 DILAAFDLAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 337
Query: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 360
VTNVAPWQITVGASTMDRKFPSLVVLGN+KQIEGESLSQ+ALPSKKLYPLMNAADVRLAN
Sbjct: 338 VTNVAPWQITVGASTMDRKFPSLVVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLAN 397
Query: 361 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 420
ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI
Sbjct: 398 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 457
Query: 421 LADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 480
LADPHVLPA HIN+TDGS VFAYINSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTV
Sbjct: 458 LADPHVLPALHINYTDGSAVFAYINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 517
Query: 481 TPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 540
TPEILKPDITAPGLSVIAAY+EAEGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+T
Sbjct: 518 TPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRT 577
Query: 541 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIYDI 600
LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASY VA+PFNYGAGHVHPNGATDPGL+YDI
Sbjct: 578 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDI 637
Query: 601 EVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRLKN 660
+VNEYLSFLCALGY+KAQISQFS+GPFNCSEPIS TNLNYPSITVP+LSRSI ITRRLKN
Sbjct: 638 DVNEYLSFLCALGYDKAQISQFSDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKN 697
Query: 661 VGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYGDLIW 720
VGSPGTYE +IRKPAGISVWVKPKKLNFT+LGEELSFKVFMKVK HKVAKKNYVYGDLIW
Sbjct: 698 VGSPGTYEAKIRKPAGISVWVKPKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIW 757
Query: 721 SDGKHHVRSPIVVKVV 737
SDGKHHVRSPIVVKVV
Sbjct: 758 SDGKHHVRSPIVVKVV 773
BLAST of Chy1G005480 vs. ExPASy TrEMBL
Match:
A0A5A7UYI5 (Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G002010 PE=3 SV=1)
HSP 1 Score: 1367.4 bits (3538), Expect = 0.0e+00
Identity = 676/738 (91.60%), Postives = 700/738 (94.85%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGG+KPADVVA+SHH+FLQPFLKSGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38 MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHPRVVSVFLNRGRKLHTTRSWEFMGLE ENGVI SESIWKKARFGEDTIIGNLDTGV
Sbjct: 98 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGV 157
Query: 121 WAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
WAESKSFSDDE GPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS
Sbjct: 158 WAESKSFSDDEMGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 217
Query: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFDA 240
PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPK GNECFDA
Sbjct: 218 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 277
Query: 241 DILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
DILA FD AIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT
Sbjct: 278 DILAAFDLAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 337
Query: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIE--GESLSQDALPSKKLYPLMNAADVRL 360
VTNVAPWQITVGASTMDRKFPSLVVLGN+KQIE G + L + +N + L
Sbjct: 338 VTNVAPWQITVGASTMDRKFPSLVVLGNKKQIEETGTIIFCSLLAIEYTAYYLNYQYLTL 397
Query: 361 ANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGN 420
+ + +QLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGN
Sbjct: 398 TSWTPF-SQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGN 457
Query: 421 EILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPN 480
EILADPHVLPA HIN+TDGS VFAYINSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPN
Sbjct: 458 EILADPHVLPALHINYTDGSAVFAYINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPN 517
Query: 481 TVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLL 540
TVTPEILKPDITAPGLSVIAAY+EAEGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL
Sbjct: 518 TVTPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLL 577
Query: 541 KTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIY 600
+TLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASY VA+PFNYGAGHVHPNGATDPGL+Y
Sbjct: 578 RTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVY 637
Query: 601 DIEVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRL 660
DI+VNEYLSFLCALGY+KAQISQFS+GPFNCSEPIS TNLNYPSITVP+LSRSI ITRRL
Sbjct: 638 DIDVNEYLSFLCALGYDKAQISQFSDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRL 697
Query: 661 KNVGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYGDL 720
KNVGSPGTYE +IRKPAGISVWVKPKKLNFT+LGEELSFKVFMKVK HKVAKKNYVYGDL
Sbjct: 698 KNVGSPGTYEAKIRKPAGISVWVKPKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDL 757
Query: 721 IWSDGKHHVRSPIVVKVV 737
IWSDGKHHVRSPIVVKVV
Sbjct: 758 IWSDGKHHVRSPIVVKVV 774
BLAST of Chy1G005480 vs. ExPASy TrEMBL
Match:
A0A6J1HWD2 (subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111468122 PE=3 SV=1)
HSP 1 Score: 1321.6 bits (3419), Expect = 0.0e+00
Identity = 645/738 (87.40%), Postives = 688/738 (93.22%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGG+KPAD+VA+SH E L+P+L+SG EF DVIFYSYTRHINGFAAMLEDEVAA+
Sbjct: 57 MGAHSHGGRKPADIVADSHRELLRPYLESGNEFAEDVIFYSYTRHINGFAAMLEDEVAAR 116
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHP VVSVF NRGR+LHTTRSWEFM LE +GVI+SESIWKKARFGEDTIIGNLDTGV
Sbjct: 117 LAKHPEVVSVFPNRGRRLHTTRSWEFMELENNHGVISSESIWKKARFGEDTIIGNLDTGV 176
Query: 121 WAESKSFSD-DEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFH 180
W ESKSFSD D+ GPIP RW+GICQNQNDPSFHCNRKLIGARYFNKGY SVVG LNSSF+
Sbjct: 177 WPESKSFSDYDDLGPIPQRWRGICQNQNDPSFHCNRKLIGARYFNKGYKSVVGHLNSSFN 236
Query: 181 SPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFD 240
SPRD EGHGSHTLSTAGGNFVAGASVFGLGKG AKGGSPRARVAAYKVCWPP GNECFD
Sbjct: 237 SPRDNEGHGSHTLSTAGGNFVAGASVFGLGKGIAKGGSPRARVAAYKVCWPPAAGNECFD 296
Query: 241 ADILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAG 300
ADILA FD AIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHA++HGIVVICSAGNSGP AG
Sbjct: 297 ADILAAFDVAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAVRHGIVVICSAGNSGPTAG 356
Query: 301 TVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLA 360
+VTN+APWQITVGASTMDRKFPS VVLGN+KQIEGESL+ + LPS+K YPLM+AADVRLA
Sbjct: 357 SVTNIAPWQITVGASTMDRKFPSRVVLGNKKQIEGESLTPEGLPSRKFYPLMSAADVRLA 416
Query: 361 NASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNE 420
NASAHEAQLCKAGTLDP KAKGKILVCLRGDNARVDKGEQA+LAGA GMILANNELSGNE
Sbjct: 417 NASAHEAQLCKAGTLDPKKAKGKILVCLRGDNARVDKGEQAMLAGAVGMILANNELSGNE 476
Query: 421 ILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNT 480
ILADPHVLPASHINFTDG VFAYINSTK+P+AYIT ATTQLG RPAPFMAAFSSVGP+T
Sbjct: 477 ILADPHVLPASHINFTDGVAVFAYINSTKFPQAYITGATTQLGTRPAPFMAAFSSVGPST 536
Query: 481 VTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLK 540
+TPEILKPD+TAPGLSVIAAY+EAEGPTNQE+D RR+ FNSVSGTSMSCPHVSGIAGLL+
Sbjct: 537 ITPEILKPDVTAPGLSVIAAYTEAEGPTNQEYDKRRVAFNSVSGTSMSCPHVSGIAGLLR 596
Query: 541 TLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIYD 600
TLYPHWSPAAIKSAIMTTASTLDNNF+PLLNASYSVA+PFNYGAGH+HPN ATDPGL+YD
Sbjct: 597 TLYPHWSPAAIKSAIMTTASTLDNNFKPLLNASYSVATPFNYGAGHIHPNKATDPGLVYD 656
Query: 601 IEVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRLK 660
I + EYLSFLCALGYNKAQ+SQFSNGPFNCSEPIS TNLNYPSITVP LSRSI ITRRLK
Sbjct: 657 ITITEYLSFLCALGYNKAQLSQFSNGPFNCSEPISLTNLNYPSITVPNLSRSITITRRLK 716
Query: 661 NVGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAK-KNYVYGDL 720
NVGSPGTY+ EIR+PAGISV VKPKKL+FTRLGEELSFKV MKVKE V + KNYVYGDL
Sbjct: 717 NVGSPGTYKAEIRRPAGISVSVKPKKLSFTRLGEELSFKVLMKVKEGNVGREKNYVYGDL 776
Query: 721 IWSDGKHHVRSPIVVKVV 737
IWSDGKHHVRSPIVV +
Sbjct: 777 IWSDGKHHVRSPIVVMAI 794
BLAST of Chy1G005480 vs. NCBI nr
Match:
XP_011658647.1 (subtilisin-like protease SBT5.3 [Cucumis sativus] >KAE8645706.1 hypothetical protein Csa_020514 [Cucumis sativus])
HSP 1 Score: 1453 bits (3762), Expect = 0.0
Identity = 713/736 (96.88%), Postives = 722/736 (98.10%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFT DVIFYSYTRHINGFAAMLEDEVA Q
Sbjct: 37 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAVQ 96
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHP+VVSVFLNRGRKLHTTRSWEFMGLE +NGVINSESIWKKARFGEDTIIGNLDTGV
Sbjct: 97 LAKHPKVVSVFLNRGRKLHTTRSWEFMGLENKNGVINSESIWKKARFGEDTIIGNLDTGV 156
Query: 121 WAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
WAESKSFSDDEYGPIPHRWKGICQNQ DPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS
Sbjct: 157 WAESKSFSDDEYGPIPHRWKGICQNQKDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 216
Query: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFDA 240
PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPK GNECFDA
Sbjct: 217 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 276
Query: 241 DILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
DILA FDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT
Sbjct: 277 DILAAFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 336
Query: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 360
VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN
Sbjct: 337 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 396
Query: 361 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 420
AS HEAQLCKAGTL+PMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI
Sbjct: 397 ASVHEAQLCKAGTLNPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 456
Query: 421 LADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 480
LADPHVLPASHINFTDGS VFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV
Sbjct: 457 LADPHVLPASHINFTDGSAVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 516
Query: 481 TPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 540
TPEILKPDITAPGLSVIAAY+EAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT
Sbjct: 517 TPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 576
Query: 541 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIYDI 600
LYPHWSPAAIKSAIMTTAS LDNNFEPLLNASYSVASPFNYGAGHVHPNGA DPGL+YDI
Sbjct: 577 LYPHWSPAAIKSAIMTTASILDNNFEPLLNASYSVASPFNYGAGHVHPNGAADPGLVYDI 636
Query: 601 EVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRLKN 660
EVNEYLSFLCALGYNKAQISQFSNGPFNCS+PISPTNLNYPSITVP+LSRSI ITRRLKN
Sbjct: 637 EVNEYLSFLCALGYNKAQISQFSNGPFNCSDPISPTNLNYPSITVPKLSRSITITRRLKN 696
Query: 661 VGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYGDLIW 720
VGSPGTY+ EIRKPAGISVWVKPKKL+FTRLGEELSFKV MKVKE KVAKKNYVYGDLIW
Sbjct: 697 VGSPGTYKAEIRKPAGISVWVKPKKLSFTRLGEELSFKVLMKVKERKVAKKNYVYGDLIW 756
Query: 721 SDGKHHVRSPIVVKVV 736
SDGKHHVRSPIVVKVV
Sbjct: 757 SDGKHHVRSPIVVKVV 772
BLAST of Chy1G005480 vs. NCBI nr
Match:
TYK03381.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa])
HSP 1 Score: 1438 bits (3723), Expect = 0.0
Identity = 704/736 (95.65%), Postives = 722/736 (98.10%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGG+KPADVVA+SHH+FLQPFLKSGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38 MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHPRVVSVFLNRGRKLHTTRSWEFMGLE ENGVI SESIWKKARFGEDTIIGNLDTGV
Sbjct: 98 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGV 157
Query: 121 WAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
WAESKSFSDDE GPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS
Sbjct: 158 WAESKSFSDDEMGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 217
Query: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFDA 240
PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPK GNECFDA
Sbjct: 218 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 277
Query: 241 DILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
DILA FD AIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT
Sbjct: 278 DILAAFDLAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 337
Query: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 360
VTNVAPWQITVGASTMDRKFPSLVVLGN+KQIEGESLSQ+ALPSKKLYPLMNAADVRLAN
Sbjct: 338 VTNVAPWQITVGASTMDRKFPSLVVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLAN 397
Query: 361 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 420
ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI
Sbjct: 398 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 457
Query: 421 LADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 480
LADPHVLPA HIN+TDGS VFAYINSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTV
Sbjct: 458 LADPHVLPALHINYTDGSAVFAYINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 517
Query: 481 TPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 540
TPEILKPDITAPGLSVIAAY+EAEGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+T
Sbjct: 518 TPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRT 577
Query: 541 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIYDI 600
LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASY VA+PFNYGAGHVHPNGATDPGL+YDI
Sbjct: 578 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDI 637
Query: 601 EVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRLKN 660
+VNEYLSFLCALGY+KAQISQFS+GPFNCSEPIS TNLNYPSITVP+LSRSI ITRRLKN
Sbjct: 638 DVNEYLSFLCALGYDKAQISQFSDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKN 697
Query: 661 VGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYGDLIW 720
VGSPGTYE +IRKPAGISVWVKPKKLNFT+LGEELSFKVFMKVK HKVAKKNYVYGDLIW
Sbjct: 698 VGSPGTYEAKIRKPAGISVWVKPKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIW 757
Query: 721 SDGKHHVRSPIVVKVV 736
SDGKHHVRSPIVVKVV
Sbjct: 758 SDGKHHVRSPIVVKVV 773
BLAST of Chy1G005480 vs. NCBI nr
Match:
XP_008462019.1 (PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo])
HSP 1 Score: 1438 bits (3723), Expect = 0.0
Identity = 704/736 (95.65%), Postives = 722/736 (98.10%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGG+KPADVVA+SHH+FLQPFLKSGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38 MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHPRVVSVFLNRGRKLHTTRSWEFMGLE ENGVI SESIWKKARFGEDTIIGNLDTGV
Sbjct: 98 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGV 157
Query: 121 WAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
WAESKSFSDDE GPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS
Sbjct: 158 WAESKSFSDDEMGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 217
Query: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFDA 240
PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPK GNECFDA
Sbjct: 218 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 277
Query: 241 DILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
DILA FD AIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT
Sbjct: 278 DILAAFDLAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 337
Query: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 360
VTNVAPWQITVGASTMDRKFPSLVVLGN+KQIEGESLSQ+ALPSKKLYPLMNAADVRLAN
Sbjct: 338 VTNVAPWQITVGASTMDRKFPSLVVLGNKKQIEGESLSQEALPSKKLYPLMNAADVRLAN 397
Query: 361 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 420
ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI
Sbjct: 398 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 457
Query: 421 LADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 480
LADPHVLPA HIN+TDGS VFAYINSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPNTV
Sbjct: 458 LADPHVLPALHINYTDGSAVFAYINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 517
Query: 481 TPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 540
TPEILKPDITAPGLSVIAAY+EAEGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL+T
Sbjct: 518 TPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLLRT 577
Query: 541 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIYDI 600
LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASY VA+PFNYGAGHVHPNGATDPGL+YDI
Sbjct: 578 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVYDI 637
Query: 601 EVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRLKN 660
+VNEYLSFLCALGY+KAQISQFS+GPFNCSEPIS TNLNYPSITVP+LSRSI ITRRLKN
Sbjct: 638 DVNEYLSFLCALGYDKAQISQFSDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRLKN 697
Query: 661 VGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYGDLIW 720
VGSPGTYE +IRKPAGISVWVKPKKLNFT+LGEELSFKVFMKVK HKVAKKNYVYGDLIW
Sbjct: 698 VGSPGTYEAKIRKPAGISVWVKPKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDLIW 757
Query: 721 SDGKHHVRSPIVVKVV 736
SDGKHHVRSPIVVKVV
Sbjct: 758 SDGKHHVRSPIVVKVV 773
BLAST of Chy1G005480 vs. NCBI nr
Match:
XP_038888965.1 (subtilisin-like protease SBT5.3 [Benincasa hispida])
HSP 1 Score: 1369 bits (3544), Expect = 0.0
Identity = 667/736 (90.62%), Postives = 703/736 (95.52%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGG+KPADVVA+SHH+FL+PFLK ++FT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 37 MGAHSHGGRKPADVVADSHHKFLRPFLKR-DDFTEDVIFYSYTRHINGFAAMLEDEVAAQ 96
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHP+VVS+FLN+GRKLHTTRSWEFMG+E NGVI SESIWKKARFGEDTIIGNLDTGV
Sbjct: 97 LAKHPKVVSIFLNKGRKLHTTRSWEFMGMENNNGVITSESIWKKARFGEDTIIGNLDTGV 156
Query: 121 WAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
W ESKSFSDD+ GPIP RW+GICQNQNDPSFHCNRKLIGARYFNKGY SVVGPLNSSF+S
Sbjct: 157 WPESKSFSDDDLGPIPLRWRGICQNQNDPSFHCNRKLIGARYFNKGYESVVGPLNSSFNS 216
Query: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFDA 240
PRDKEGHGSHTLSTAGGNFVAGASVFGLG GTAKGGSPRARVAAYKVCWPP GNECFDA
Sbjct: 217 PRDKEGHGSHTLSTAGGNFVAGASVFGLGNGTAKGGSPRARVAAYKVCWPPAAGNECFDA 276
Query: 241 DILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
DILA FD AIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHA+KHGIVVICSAGNSGPAAGT
Sbjct: 277 DILAAFDSAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAVKHGIVVICSAGNSGPAAGT 336
Query: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRLAN 360
VTN+APWQITVGASTMDRKFPSLVVLGN+KQIEGESLSQ+ALPS+K YPL++AADVRLAN
Sbjct: 337 VTNIAPWQITVGASTMDRKFPSLVVLGNKKQIEGESLSQNALPSRKFYPLVSAADVRLAN 396
Query: 361 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGNEI 420
ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGA GMILANNE SGNEI
Sbjct: 397 ASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAVGMILANNEPSGNEI 456
Query: 421 LADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPNTV 480
LADPHVLPASHINFTDG+ V YINSTK+P+AYIT ATTQLGIRPAPFMAAFSSVGPNT+
Sbjct: 457 LADPHVLPASHINFTDGTAVLTYINSTKFPKAYITRATTQLGIRPAPFMAAFSSVGPNTI 516
Query: 481 TPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKT 540
PEILKPD+TAPGLSVIAAY+EAEGPTNQE+DNRR+PFNSVSGTSMSCPHVSGIAGLL+T
Sbjct: 517 IPEILKPDVTAPGLSVIAAYTEAEGPTNQEYDNRRLPFNSVSGTSMSCPHVSGIAGLLRT 576
Query: 541 LYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIYDI 600
LYPHWSPAAIKSAIMTTA+TLDNNFEPLLNASYSVA+P NYGAGHVHPNGATDPGLIYDI
Sbjct: 577 LYPHWSPAAIKSAIMTTANTLDNNFEPLLNASYSVATPLNYGAGHVHPNGATDPGLIYDI 636
Query: 601 EVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRLKN 660
EVNEYL FLCALGYN+AQISQFS+GPFNCSEPI+ TNLNYPSITVPELSRSI ITRRLKN
Sbjct: 637 EVNEYLLFLCALGYNQAQISQFSSGPFNCSEPINLTNLNYPSITVPELSRSITITRRLKN 696
Query: 661 VGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYGDLIW 720
VGSPGTY+ +IRKPAGISVWVKPKKLNFTRLGEE SFKV MKVKE AKKNYVYGDLIW
Sbjct: 697 VGSPGTYKAQIRKPAGISVWVKPKKLNFTRLGEEQSFKVLMKVKEQNAAKKNYVYGDLIW 756
Query: 721 SDGKHHVRSPIVVKVV 736
SDGKHHVRSPIVVKVV
Sbjct: 757 SDGKHHVRSPIVVKVV 771
BLAST of Chy1G005480 vs. NCBI nr
Match:
KAA0059266.1 (subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa])
HSP 1 Score: 1366 bits (3536), Expect = 0.0
Identity = 676/738 (91.60%), Postives = 700/738 (94.85%), Query Frame = 0
Query: 1 MGAHSHGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQ 60
MGAHSHGG+KPADVVA+SHH+FLQPFLKSGEEFT DVIFYSYTRHINGFAAMLEDEVAAQ
Sbjct: 38 MGAHSHGGRKPADVVADSHHQFLQPFLKSGEEFTKDVIFYSYTRHINGFAAMLEDEVAAQ 97
Query: 61 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGV 120
LAKHPRVVSVFLNRGRKLHTTRSWEFMGLE ENGVI SESIWKKARFGEDTIIGNLDTGV
Sbjct: 98 LAKHPRVVSVFLNRGRKLHTTRSWEFMGLENENGVIESESIWKKARFGEDTIIGNLDTGV 157
Query: 121 WAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 180
WAESKSFSDDE GPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS
Sbjct: 158 WAESKSFSDDEMGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSFHS 217
Query: 181 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECFDA 240
PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPK GNECFDA
Sbjct: 218 PRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKAGNECFDA 277
Query: 241 DILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 300
DILA FD AIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT
Sbjct: 278 DILAAFDLAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAAGT 337
Query: 301 VTNVAPWQITVGASTMDRKFPSLVVLGNRKQIE--GESLSQDALPSKKLYPLMNAADVRL 360
VTNVAPWQITVGASTMDRKFPSLVVLGN+KQIE G + L + +N + L
Sbjct: 338 VTNVAPWQITVGASTMDRKFPSLVVLGNKKQIEETGTIIFCSLLAIEYTAYYLNYQYLTL 397
Query: 361 ANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGN 420
+ + +QLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGN
Sbjct: 398 TSWTPF-SQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGN 457
Query: 421 EILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPN 480
EILADPHVLPA HIN+TDGS VFAYINSTK+PEAYITPATTQLGIRPAPFMAAFSSVGPN
Sbjct: 458 EILADPHVLPALHINYTDGSAVFAYINSTKFPEAYITPATTQLGIRPAPFMAAFSSVGPN 517
Query: 481 TVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLL 540
TVTPEILKPDITAPGLSVIAAY+EAEGPTNQEFDNRR+PFNSVSGTSMSCPHVSGIAGLL
Sbjct: 518 TVTPEILKPDITAPGLSVIAAYTEAEGPTNQEFDNRRVPFNSVSGTSMSCPHVSGIAGLL 577
Query: 541 KTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIY 600
+TLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASY VA+PFNYGAGHVHPNGATDPGL+Y
Sbjct: 578 RTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYFVATPFNYGAGHVHPNGATDPGLVY 637
Query: 601 DIEVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISPTNLNYPSITVPELSRSIAITRRL 660
DI+VNEYLSFLCALGY+KAQISQFS+GPFNCSEPIS TNLNYPSITVP+LSRSI ITRRL
Sbjct: 638 DIDVNEYLSFLCALGYDKAQISQFSDGPFNCSEPISLTNLNYPSITVPKLSRSITITRRL 697
Query: 661 KNVGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYGDL 720
KNVGSPGTYE +IRKPAGISVWVKPKKLNFT+LGEELSFKVFMKVK HKVAKKNYVYGDL
Sbjct: 698 KNVGSPGTYEAKIRKPAGISVWVKPKKLNFTKLGEELSFKVFMKVKGHKVAKKNYVYGDL 757
Query: 721 IWSDGKHHVRSPIVVKVV 736
IWSDGKHHVRSPIVVKVV
Sbjct: 758 IWSDGKHHVRSPIVVKVV 774
BLAST of Chy1G005480 vs. TAIR 10
Match:
AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )
HSP 1 Score: 957.6 bits (2474), Expect = 5.8e-279
Identity = 474/739 (64.14%), Postives = 572/739 (77.40%), Query Frame = 0
Query: 2 GAHSHGGQKPADV---VANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVA 61
GAHSH G+ D V +H++FL F S E T D IFYSYT+HINGFAA L+ ++A
Sbjct: 37 GAHSHVGEITEDAMDRVKETHYDFLGSFTGSRERAT-DAIFYSYTKHINGFAAHLDHDLA 96
Query: 62 AQLAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDT 121
+++KHP VVSVF N+ KLHTTRSW+F+GLE N + S SIW+KARFGEDTII NLDT
Sbjct: 97 YEISKHPEVVSVFPNKALKLHTTRSWDFLGLE-HNSYVPSSSIWRKARFGEDTIIANLDT 156
Query: 122 GVWAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVGPLNSSF 181
GVW ESKSF D+ GPIP RWKGICQNQ D +FHCNRKLIGARYFNKGYA+ VG LNSSF
Sbjct: 157 GVWPESKSFRDEGLGPIPSRWKGICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSF 216
Query: 182 HSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECF 241
SPRD +GHGSHTLSTA G+FV G S+FG G GTAKGGSPRARVAAYKVCWPP GNEC+
Sbjct: 217 DSPRDLDGHGSHTLSTAAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECY 276
Query: 242 DADILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAA 301
DAD+LA FD AIHDG DV+SVSLGG+P FNDSVAIGSFHA K IVV+CSAGNSGPA
Sbjct: 277 DADVLAAFDAAIHDGADVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPAD 336
Query: 302 GTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADVRL 361
TV+NVAPWQITVGASTMDR+F S +VLGN K +G+SLS ALP K YP+M + + +
Sbjct: 337 STVSNVAPWQITVGASTMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKA 396
Query: 362 ANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSGN 421
NASA +AQLCK G+LDP+K KGKILVCLRG N RV+KG L G GM+L N ++GN
Sbjct: 397 KNASALDAQLCKLGSLDPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGN 456
Query: 422 EILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGPN 481
++LADPHVLPA+ + D V YI+ TK P A+ITP+ T LG++PAP MA+FSS GP+
Sbjct: 457 DLLADPHVLPATQLTSKDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPS 516
Query: 482 TVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGLL 541
V P+ILKPDITAPG+SVIAAY+ A PTN++FD RR+ FN++SGTSMSCPH+SGIAGLL
Sbjct: 517 IVAPQILKPDITAPGVSVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLL 576
Query: 542 KTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGLIY 601
KT YP WSPAAI+SAIMTTA+ +D+ P+ NA+ A+PF++GAGHV PN A +PGL+Y
Sbjct: 577 KTRYPSWSPAAIRSAIMTTATIMDDIPGPIQNATNMKATPFSFGAGHVQPNLAVNPGLVY 636
Query: 602 DIEVNEYLSFLCALGYNKAQISQFSNGPFNCSEP-ISPTNLNYPSITVPELSRS-IAITR 661
D+ + +YL+FLC+LGYN +QIS FS F CS P IS NLNYPSITVP L+ S + ++R
Sbjct: 637 DLGIKDYLNFLCSLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSR 696
Query: 662 RLKNVGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVYG 721
+KNVG P Y V++ P G+ V VKP LNFT++GE+ +FKV + + VA K YV+G
Sbjct: 697 TVKNVGRPSMYTVKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVA-KGYVFG 756
Query: 722 DLIWSDGKHHVRSPIVVKV 736
+L+WSD KH VRSPIVVK+
Sbjct: 757 ELVWSDKKHRVRSPIVVKL 772
BLAST of Chy1G005480 vs. TAIR 10
Match:
AT5G59810.1 (Subtilase family protein )
HSP 1 Score: 890.2 bits (2299), Expect = 1.1e-258
Identity = 439/740 (59.32%), Postives = 554/740 (74.86%), Query Frame = 0
Query: 1 MGAHSHGGQKPA---DVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEV 60
+G+H+H Q + D VA+SH FL F+ S E + IFYSY RHINGFAA+L++
Sbjct: 46 LGSHAHLPQISSAHLDGVAHSHRTFLASFVGSHEN-AKEAIFYSYKRHINGFAAILDENE 105
Query: 61 AAQLAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLD 120
AA++AKHP VVSVF N+GRKLHTT SW FM L K NGV++ S+W KA +GEDTII NLD
Sbjct: 106 AAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLLAK-NGVVHKSSLWNKAGYGEDTIIANLD 165
Query: 121 TGVWAESKSFSDDEYGPIPHRWKGICQNQNDPSFHCNRKLIGARYFNKGYASVVG-PLNS 180
TGVW ESKSFSD+ YG +P RWKG C CNRKLIGARYFNKGY + G P N+
Sbjct: 166 TGVWPESKSFSDEGYGAVPARWKGRCHK----DVPCNRKLIGARYFNKGYLAYTGLPSNA 225
Query: 181 SFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNE 240
S+ + RD +GHGSHTLSTA GNFV GA+VFG+G GTA GGSP+ARVAAYKVCWPP G E
Sbjct: 226 SYETCRDHDGHGSHTLSTAAGNFVPGANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAE 285
Query: 241 CFDADILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGP 300
CFDADILA + AI DGVDVLS S+GGD +D +AIGSFHA+K+G+ V+CSAGNSGP
Sbjct: 286 CFDADILAAIEAAIEDGVDVLSASVGGDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGP 345
Query: 301 AAGTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQDALPSKKLYPLMNAADV 360
+GTV+NVAPW ITVGAS+MDR+F + V L N + +G SLS+ LP +K+Y L++AAD
Sbjct: 346 KSGTVSNVAPWVITVGASSMDREFQAFVELKNGQSFKGTSLSK-PLPEEKMYSLISAADA 405
Query: 361 RLANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELS 420
+AN + +A LCK G+LDP K KGKILVCLRGDNARVDKG QA AGAAGM+L N++ S
Sbjct: 406 NVANGNVTDALLCKKGSLDPKKVKGKILVCLRGDNARVDKGMQAAAAGAAGMVLCNDKAS 465
Query: 421 GNEILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVG 480
GNEI++D HVLPAS I++ DG +F+Y++STK P+ YI T L +PAPFMA+FSS G
Sbjct: 466 GNEIISDAHVLPASQIDYKDGETLFSYLSSTKDPKGYIKAPTATLNTKPAPFMASFSSRG 525
Query: 481 PNTVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAG 540
PNT+TP ILKPDITAPG+++IAA++EA GPT+ + DNRR PFN+ SGTSMSCPH+SG+ G
Sbjct: 526 PNTITPGILKPDITAPGVNIIAAFTEATGPTDLDSDNRRTPFNTESGTSMSCPHISGVVG 585
Query: 541 LLKTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVASPFNYGAGHVHPNGATDPGL 600
LLKTL+PHWSPAAI+SAIMTT+ T +N +P+++ S+ A+PF+YG+GHV PN A PGL
Sbjct: 586 LLKTLHPHWSPAAIRSAIMTTSRTRNNRRKPMVDESFKKANPFSYGSGHVQPNKAAHPGL 645
Query: 601 IYDIEVNEYLSFLCALGYNKAQISQFSNGP-FNCSEPISPTNLNYPSITVPELSRSIAIT 660
+YD+ +YL FLCA+GYN + F+ P + C + + + NYPSITVP L+ SI +T
Sbjct: 646 VYDLTTGDYLDFLCAVGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVT 705
Query: 661 RRLKNVGSPGTYEVEIRKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKNYVY 720
R+LKNVG P TY R+P G+ V V+PK+L F + GE F+ M ++ V YV+
Sbjct: 706 RKLKNVGPPATYNARFREPLGVRVSVEPKQLTFNKTGEVKIFQ--MTLRPLPVTPSGYVF 765
Query: 721 GDLIWSDGKHHVRSPIVVKV 736
G+L W+D H+VRSPIVV++
Sbjct: 766 GELTWTDSHHYVRSPIVVQL 776
BLAST of Chy1G005480 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 614.0 bits (1582), Expect = 1.6e-175
Identity = 342/741 (46.15%), Postives = 472/741 (63.70%), Query Frame = 0
Query: 4 HSHGGQKPA--DVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQL 63
H Q P+ D+ +N + L+ S E + Y+Y I+GF+ L E A L
Sbjct: 35 HMAKSQMPSSFDLHSNWYDSSLRSISDSAE------LLYTYENAIHGFSTRLTQEEADSL 94
Query: 64 AKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVINSESIWKKARFGEDTIIGNLDTGVW 123
P V+SV +LHTTR+ F+GL++ ++ ++ +A D ++G LDTGVW
Sbjct: 95 MTQPGVISVLPEHRYELHTTRTPLFLGLDE-----HTADLFPEAGSYSDVVVGVLDTGVW 154
Query: 124 AESKSFSDDEYGPIPHRWKGICQ-NQNDPSFHCNRKLIGARYFNKGYASVVGPLNSS--F 183
ESKS+SD+ +GPIP WKG C+ N + CNRKLIGAR+F +GY S +GP++ S
Sbjct: 155 PESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKES 214
Query: 184 HSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAKGGSPRARVAAYKVCWPPKGGNECF 243
SPRD +GHG+HT STA G+ V GAS+ G GTA+G +PRARVA YKVCW GG CF
Sbjct: 215 RSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCW--LGG--CF 274
Query: 244 DADILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVAIGSFHAIKHGIVVICSAGNSGPAA 303
+DILA D AI D V+VLS+SLGG + + D VAIG+F A++ GI+V CSAGN+GP++
Sbjct: 275 SSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSS 334
Query: 304 GTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESLSQ-DALPSKKLYPLMNAADVR 363
+++NVAPW TVGA T+DR FP+L +LGN K G SL + +ALP KL P + A +
Sbjct: 335 SSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPD-KLLPFIYAGNA- 394
Query: 364 LANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKGEQALLAGAAGMILANNELSG 423
++A LC GTL P K KGKI++C RG NARV KG+ AG GMILAN +G
Sbjct: 395 ---SNATNGNLCMTGTLIPEKVKGKIVMCDRGINARVQKGDVVKAAGGVGMILANTAANG 454
Query: 424 NEILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPATTQLGIRPAPFMAAFSSVGP 483
E++AD H+LPA+ + G ++ Y+ + P A I+ T +G++P+P +AAFSS GP
Sbjct: 455 EELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGP 514
Query: 484 NTVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIPFNSVSGTSMSCPHVSGIAGL 543
N++TP ILKPD+ APG++++AA++ A GPT D+RR+ FN +SGTSMSCPHVSG+A L
Sbjct: 515 NSITPNILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAAL 574
Query: 544 LKTLYPHWSPAAIKSAIMTTASTLDNNFEPLLN-ASYSVASPFNYGAGHVHPNGATDPGL 603
LK+++P WSPAAI+SA+MTTA + +PLL+ A+ ++PF++GAGHV P AT+PGL
Sbjct: 575 LKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGL 634
Query: 604 IYDIEVNEYLSFLCALGYNKAQISQFSNGPFNC--SEPISPTNLNYPSITV-PELSRSIA 663
IYD+ +YL FLCAL Y QI S + C S+ S +LNYPS V + +
Sbjct: 635 IYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYK 694
Query: 664 ITRRLKNVGSPGTYEVEI-RKPAGISVWVKPKKLNFTRLGEELSFKVFMKVKEHKVAKKN 723
TR + +VG GTY V++ + G+ + V+P LNF E+ S+ V V K + N
Sbjct: 695 YTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSN 754
Query: 724 YVYGDLIWSDGKHHVRSPIVV 734
+G + WSDGKH V SP+ +
Sbjct: 755 -SFGSIEWSDGKHVVGSPVAI 754
BLAST of Chy1G005480 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 606.7 bits (1563), Expect = 2.5e-173
Identity = 335/718 (46.66%), Postives = 452/718 (62.95%), Query Frame = 0
Query: 38 IFYSYTRHINGFAAMLEDEVAAQLAKHPRVVSVFLNRGRKLHTTRSWEFMGLEKENGVIN 97
I ++Y +GF+A L + A+QL HP V+SV + R LHTTRS EF+GL +
Sbjct: 62 IIHTYDTVFHGFSARLTSQDASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRS----TD 121
Query: 98 SESIWKKARFGEDTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGIC-QNQNDPSFHCNRK 157
+ +++ FG D +IG +DTGVW E SF D GP+P +WKG C +Q+ P CNRK
Sbjct: 122 KAGLLEESDFGSDLVIGVIDTGVWPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRK 181
Query: 158 LIGARYFNKGYASVVGPLN--SSFHSPRDKEGHGSHTLSTAGGNFVAGASVFGLGKGTAK 217
L+GAR+F GY + G +N + F SPRD +GHG+HT S + G +V AS G G A
Sbjct: 182 LVGARFFCGGYEATNGKMNETTEFRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAA 241
Query: 218 GGSPRARVAAYKVCWPPKGGNECFDADILAGFDFAIHDGVDVLSVSLGGDPNPLFNDSVA 277
G +P+AR+AAYKVCW + C+D+DILA FD A+ DGVDV+S+S+GG P + D++A
Sbjct: 242 GMAPKARLAAYKVCW----NSGCYDSDILAAFDTAVADGVDVISLSVGGVVVPYYLDAIA 301
Query: 278 IGSFHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEG 337
IG+F AI GI V SAGN GP A TVTNVAPW TVGA T+DR FP+ V LGN K I G
Sbjct: 302 IGAFGAIDRGIFVSASAGNGGPGALTVTNVAPWMTTVGAGTIDRDFPANVKLGNGKMISG 361
Query: 338 ESL-SQDALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNAR 397
S+ L ++YPL+ L + + LC G+LDP KGKI++C RG N+R
Sbjct: 362 VSVYGGPGLDPGRMYPLVYGGS--LLGGDGYSSSLCLEGSLDPNLVKGKIVLCDRGINSR 421
Query: 398 VDKGEQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSLVFAYIN------ST 457
KGE G GMI+AN G ++AD HVLPA+ + + G + YI+ S+
Sbjct: 422 ATKGEIVRKNGGLGMIIANGVFDGEGLVADCHVLPATSVGASGGDEIRRYISESSKSRSS 481
Query: 458 KYPEAYITPATTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYSEAEGPT 517
K+P A I T+LGIRPAP +A+FS+ GPN TPEILKPD+ APGL+++AA+ + GP+
Sbjct: 482 KHPTATIVFKGTRLGIRPAPVVASFSARGPNPETPEILKPDVIAPGLNILAAWPDRIGPS 541
Query: 518 NQEFDNRRIPFNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASTLDNNFEP 577
DNRR FN +SGTSM+CPHVSG+A LLK +P WSPAAI+SA++TTA T+DN+ EP
Sbjct: 542 GVTSDNRRTEFNILSGTSMACPHVSGLAALLKAAHPDWSPAAIRSALITTAYTVDNSGEP 601
Query: 578 LLNASY-SVASPFNYGAGHVHPNGATDPGLIYDIEVNEYLSFLCALGYNKAQISQFSNGP 637
+++ S + +S +YG+GHVHP A DPGL+YDI +Y++FLC Y + I +
Sbjct: 602 MMDESTGNTSSVMDYGSGHVHPTKAMDPGLVYDITSYDYINFLCNSNYTRTNIVTITRRQ 661
Query: 638 FNCS---EPISPTNLNYPSITV-----PELSRSIAITRRLKNVG-SPGTYEVEIRKPAGI 697
+C NLNYPS +V E S R + NVG S YE++IR P G
Sbjct: 662 ADCDGARRAGHVGNLNYPSFSVVFQQYGESKMSTHFIRTVTNVGDSDSVYEIKIRPPRGT 721
Query: 698 SVWVKPKKLNFTRLGEELSFKVFMKVKEHKVA--KKNYVYGDLIWSDGKHHVRSPIVV 734
+V V+P+KL+F R+G++LSF V +K E K++ N G ++WSDGK +V SP+VV
Sbjct: 722 TVTVEPEKLSFRRVGQKLSFVVRVKTTEVKLSPGATNVETGHIVWSDGKRNVTSPLVV 769
BLAST of Chy1G005480 vs. TAIR 10
Match:
AT5G45650.1 (subtilase family protein )
HSP 1 Score: 588.2 bits (1515), Expect = 9.3e-168
Identity = 335/766 (43.73%), Postives = 448/766 (58.49%), Query Frame = 0
Query: 6 HGGQKPADVVANSHHEFLQPFLKSGEEFTNDVIFYSYTRHINGFAAMLEDEVAAQLAKHP 65
H G K + HH +LQ +K EE + YSY INGFAA L + A++L K
Sbjct: 34 HKGDKAFHEIEEHHHSYLQS-VKESEEDARASLLYSYKHSINGFAAELTPDQASKLEKLA 93
Query: 66 RVVSVFLNRGRK--LHTTRSWEFMGLEKENGVIN--------------SESIWKKARFGE 125
VVSVF + RK HTTRSWEF+GLE+E + + KKA+ G+
Sbjct: 94 EVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSDVPRRKNDADDRFRVGRNFLKKAKHGD 153
Query: 126 DTIIGNLDTGVWAESKSFSDDEYGPIPHRWKGICQ-NQNDPSFHCNRKLIGARYFNKGYA 185
I+G LD+GVW ESKSF+D GP+P WKGICQ S HCNRK+IGARY+ KGY
Sbjct: 154 GIIVGVLDSGVWPESKSFNDKGMGPVPKSWKGICQTGVAFNSSHCNRKIIGARYYVKGYE 213
Query: 186 SVVGPLNSS----FHSPRDKEGHGSHTLSTAGGNFVAGASVF-GLGKGTAKGGSPRARVA 245
G N++ F SPRD +GHGSHT STA G V GAS G KG+A GG+P AR+A
Sbjct: 214 RYYGAFNATANKDFLSPRDPDGHGSHTASTAVGRRVLGASALGGFAKGSASGGAPLARLA 273
Query: 246 AYKVCWPPKG-----GNECFDADILAGFDFAIHDGVDVLSVSLG-GDPNPLFNDSVAIGS 305
YK CW GN C + D+LA D AI DGV V+S+S+G +P P D +A+G+
Sbjct: 274 IYKACWAKPNAEKVEGNICLEEDMLAAIDDAIADGVHVISISIGTTEPFPFTQDGIAMGA 333
Query: 306 FHAIKHGIVVICSAGNSGPAAGTVTNVAPWQITVGASTMDRKFPSLVVLGNRKQIEGESL 365
HA+K IVV SAGNSGP GT++N+APW ITVGAST+DR F +VLGN I+ +S+
Sbjct: 334 LHAVKRNIVVAASAGNSGPKPGTLSNLAPWIITVGASTLDRAFVGGLVLGNGYTIKTDSI 393
Query: 366 SQDALPSKKLYPLMNAADVRLANASAHEAQLCKAGTLDPMKAKGKILVCLRGDNARVDKG 425
+ A K PL+ A++V + + +E C +L P GK+++CLRG +R+ KG
Sbjct: 394 T--AFKMDKFAPLVYASNVVVPGIALNETSQCLPNSLKPELVSGKVVLCLRGAGSRIGKG 453
Query: 426 EQALLAGAAGMILANNELSGNEILADPHVLPASHINFTDGSLVFAYINSTKYPEAYITPA 485
+ AG AGMIL N +GNE+ +D H +P + + T + YI + K P+A+I P
Sbjct: 454 MEVKRAGGAGMILGNIAANGNEVPSDSHFVPTAGVTPTVVDKILEYIKTDKNPKAFIKPG 513
Query: 486 TTQLGIRPAPFMAAFSSVGPNTVTPEILKPDITAPGLSVIAAYSEAEGPTNQEFDNRRIP 545
T + AP M FSS GPN V P ILKPDITAPGL ++AA+S A+ P+ D R
Sbjct: 514 KTVYKYQAAPSMTGFSSRGPNVVDPNILKPDITAPGLYILAAWSGADSPSKMSVDQRVAG 573
Query: 546 FNSVSGTSMSCPHVSGIAGLLKTLYPHWSPAAIKSAIMTTASTLDNNFEPLLNASYSVAS 605
+N SGTSMSCPHV+G LLK ++P WS AAI+SA+MTTA ++ +P+ + + A+
Sbjct: 574 YNIYSGTSMSCPHVAGAIALLKAIHPKWSSAAIRSALMTTAWMTNDKKKPIQDTTGLPAN 633
Query: 606 PFNYGAGHVHPNGATDPGLIYDIEVNEYLSFLCALGYNKAQISQFSNGPFNCSEPISP-T 665
PF G+GH P A DPGL+YD YL + C++ + F C I P
Sbjct: 634 PFALGSGHFRPTKAADPGLVYDASYRAYLLYGCSVNITNI------DPTFKCPSKIPPGY 693
Query: 666 NLNYPSITVPELSRSIAITRRLKNVG---SPGTYEVEIRKPAGISVWVKPKKLNFTRLGE 725
N NYPSI VP L +++ + R + NVG S TY ++ P+GISV P L+F R+G+
Sbjct: 694 NHNYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQ 753
Query: 726 ELSFKVFMKVKEHKV----AKKNYVYGDLIWSDGKHHVRSPIVVKV 736
+ FK+ +K +++V K Y +G W+D H VRSPI V +
Sbjct: 754 KQRFKIVIKPLKNQVMNATEKGQYQFGWFSWTDKVHVVRSPIAVSL 790
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q9ZSP5 | 8.2e-278 | 64.14 | Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=... | [more] |
F4JXC5 | 1.6e-257 | 59.32 | Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S... | [more] |
I1N462 | 1.3e-198 | 50.26 | Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P... | [more] |
O65351 | 2.2e-174 | 46.15 | Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 S... | [more] |
Q9LUM3 | 3.5e-172 | 46.66 | Subtilisin-like protease SBT1.5 OS=Arabidopsis thaliana OX=3702 GN=SBT1.5 PE=2 S... | [more] |
Match Name | E-value | Identity | Description | |
A0A0A0K313 | 0.0e+00 | 96.88 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G030520 PE=3 SV=1 | [more] |
A0A5D3BUG6 | 0.0e+00 | 95.65 | Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=119469... | [more] |
A0A1S3CG03 | 0.0e+00 | 95.65 | subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo OX=3656 GN=LOC1035004... | [more] |
A0A5A7UYI5 | 0.0e+00 | 91.60 | Subtilisin-like protease SBT5.3 isoform X1 OS=Cucumis melo var. makuwa OX=119469... | [more] |
A0A6J1HWD2 | 0.0e+00 | 87.40 | subtilisin-like protease SBT5.3 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... | [more] |
Match Name | E-value | Identity | Description | |
XP_011658647.1 | 0.0 | 96.88 | subtilisin-like protease SBT5.3 [Cucumis sativus] >KAE8645706.1 hypothetical pro... | [more] |
TYK03381.1 | 0.0 | 95.65 | subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa] | [more] |
XP_008462019.1 | 0.0 | 95.65 | PREDICTED: subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo] | [more] |
XP_038888965.1 | 0.0 | 90.63 | subtilisin-like protease SBT5.3 [Benincasa hispida] | [more] |
KAA0059266.1 | 0.0 | 91.60 | subtilisin-like protease SBT5.3 isoform X1 [Cucumis melo var. makuwa] | [more] |