Chy1G004660 (gene) Cucumber (hystrix) v1

Overview


Name	Chy1G004660
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	O-methyltransferase
Location	chrH01: 3372922 .. 3374485 (-)
RNA-Seq Expression	Chy1G004660
Synteny	Chy1G004660

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATATGGAGGGTGGAAAGTTGTTAGCAATGGGAGGGGATGAGTTGTTAGAAGCTCAATCCCACATATGGAATCACATCTTCAACTTCATAAACTCAATGTCTCTCAAATGTGCAATCCAACTTGGAATTCCAGACGCCATCCATAGCCATGGCCCCAACCCTATGCCCCTCTCTCTTCTTGTTTCATCTCTCCAACTTCACCCTAATAAAACCCAATTCATTTACCGTTTGATGCGTTTACTAACTCACTCTGGCTTCTTTGTTCAACAAGAAGAAGGATATATTCTTACCAATTCATCTCGCCTTCTTCTTAAAGACAATCCTTGTGCTGTATCTCCTTTCCTACTTTCCATGCTCGAACCAGCCCTCACAGATCCATGGCAGTTTCTCTCAATCTGGTTTCAAACTGACGACCGAACACCATTCGAGACGGCACATGGAATGCCGTTTTGGGAGTACACGGGGAATAAGCCAAAAGAAGGGGAGGTTTTTAATGCAGGCATGGCGAGTGATGCGAGGTTGGTGATGAGTGTGTTATTGGGGAAACATAAAAGTGTTTTTGAAGGAGTTGAGTCGTTGGTTGATGTTGGTGGTGGGACTGGAACAATGGCCAAAGCCATTTCAAAAGCTTTTCCACAAATGGAATGCACTGTGTTTGATCTTCCCCCAGTAGTGGCTCATTTGAAGGGAGATCAACCAAACCTCAACTATGTTGAAGGAGATATGTTTAAAGTTATTCCTCCTGCTGATGTGCTTCTCTTAAAGGTAAACTAACCTAATTATTATTATTCTTTTCCATCGTATTCCCACGATCTACGTATAAGATAATTGTAAAGTTAAAAGAAATATATATCCTAAAATCATATATTTCCTTCAAATTTTTCGTGTATCCTTTTAAGGGTAGCCATCAAATCCTTCCATAAACTCATAATATATCTTCATCGTTGAAGAATTAAATTTAAGAATCTTTATATTGTTGGTTTGATATTTTATACTTTAACGAATTATATTTAGTATCTTTATGTTATATATATTAAAAACAACATATCTCTTAGATCGATATATCTGTACCTTAGTTTTTCAGAAATTAACGAATCGACGTATCCATATTGTGTATATGTATCTATATCTAAGTTTCATAGCATGCAATATCCCTTATACACACATTTTCCCCCTTTGTATGAGCTTATGAAATATAATATAATCCCTGTGTATTGTGATAAAATATAAGATAATGTGGAGTGTGGCATGAATAATACAGTGGATATTACATGATTGGAGTGACGAAGAATGTGTGGAGATTCTGAAGAACTGCAAAGCAGCCATTAGAAGCAATGGTTACAAAGGAAAAGTGATGGTAATTGATATTGTACTGTTTGGGAATTATAGAAAGGACTCAATGGAAACTCAATTGTTGTTCGATATGTTGATGATGACATTAACTGGTGGAAAAGAAAGGGAAGAAAAAGAGTGGGCTCAATTGATTAAAGAAGCTGGATTTGGGAGCTACAAGATCTTTCCCATTATGGGTGTGAGATCTCTTATAGAGATTTACCCATAA

mRNA sequence

ATGAATATGGAGGGTGGAAAGTTGTTAGCAATGGGAGGGGATGAGTTGTTAGAAGCTCAATCCCACATATGGAATCACATCTTCAACTTCATAAACTCAATGTCTCTCAAATGTGCAATCCAACTTGGAATTCCAGACGCCATCCATAGCCATGGCCCCAACCCTATGCCCCTCTCTCTTCTTGTTTCATCTCTCCAACTTCACCCTAATAAAACCCAATTCATTTACCGTTTGATGCGTTTACTAACTCACTCTGGCTTCTTTGTTCAACAAGAAGAAGGATATATTCTTACCAATTCATCTCGCCTTCTTCTTAAAGACAATCCTTGTGCTGTATCTCCTTTCCTACTTTCCATGCTCGAACCAGCCCTCACAGATCCATGGCAGTTTCTCTCAATCTGGTTTCAAACTGACGACCGAACACCATTCGAGACGGCACATGGAATGCCGTTTTGGGAGTACACGGGGAATAAGCCAAAAGAAGGGGAGGTTTTTAATGCAGGCATGGCGAGTGATGCGAGGTTGGTGATGAGTGTGTTATTGGGGAAACATAAAAGTGTTTTTGAAGGAGTTGAGTCGTTGGTTGATGTTGGTGGTGGGACTGGAACAATGGCCAAAGCCATTTCAAAAGCTTTTCCACAAATGGAATGCACTGTGTTTGATCTTCCCCCAGTAGTGGCTCATTTGAAGGGAGATCAACCAAACCTCAACTATGTTGAAGGAGATATGTTTAAAGTTATTCCTCCTGCTGATGTGCTTCTCTTAAAGTGGATATTACATGATTGGAGTGACGAAGAATGTGTGGAGATTCTGAAGAACTGCAAAGCAGCCATTAGAAGCAATGGTTACAAAGGAAAAGTGATGGTAATTGATATTGTACTGTTTGGGAATTATAGAAAGGACTCAATGGAAACTCAATTGTTGTTCGATATGTTGATGATGACATTAACTGGTGGAAAAGAAAGGGAAGAAAAAGAGTGGGCTCAATTGATTAAAGAAGCTGGATTTGGGAGCTACAAGATCTTTCCCATTATGGGTGTGAGATCTCTTATAGAGATTTACCCATAA

Coding sequence (CDS)

ATGAATATGGAGGGTGGAAAGTTGTTAGCAATGGGAGGGGATGAGTTGTTAGAAGCTCAATCCCACATATGGAATCACATCTTCAACTTCATAAACTCAATGTCTCTCAAATGTGCAATCCAACTTGGAATTCCAGACGCCATCCATAGCCATGGCCCCAACCCTATGCCCCTCTCTCTTCTTGTTTCATCTCTCCAACTTCACCCTAATAAAACCCAATTCATTTACCGTTTGATGCGTTTACTAACTCACTCTGGCTTCTTTGTTCAACAAGAAGAAGGATATATTCTTACCAATTCATCTCGCCTTCTTCTTAAAGACAATCCTTGTGCTGTATCTCCTTTCCTACTTTCCATGCTCGAACCAGCCCTCACAGATCCATGGCAGTTTCTCTCAATCTGGTTTCAAACTGACGACCGAACACCATTCGAGACGGCACATGGAATGCCGTTTTGGGAGTACACGGGGAATAAGCCAAAAGAAGGGGAGGTTTTTAATGCAGGCATGGCGAGTGATGCGAGGTTGGTGATGAGTGTGTTATTGGGGAAACATAAAAGTGTTTTTGAAGGAGTTGAGTCGTTGGTTGATGTTGGTGGTGGGACTGGAACAATGGCCAAAGCCATTTCAAAAGCTTTTCCACAAATGGAATGCACTGTGTTTGATCTTCCCCCAGTAGTGGCTCATTTGAAGGGAGATCAACCAAACCTCAACTATGTTGAAGGAGATATGTTTAAAGTTATTCCTCCTGCTGATGTGCTTCTCTTAAAGTGGATATTACATGATTGGAGTGACGAAGAATGTGTGGAGATTCTGAAGAACTGCAAAGCAGCCATTAGAAGCAATGGTTACAAAGGAAAAGTGATGGTAATTGATATTGTACTGTTTGGGAATTATAGAAAGGACTCAATGGAAACTCAATTGTTGTTCGATATGTTGATGATGACATTAACTGGTGGAAAAGAAAGGGAAGAAAAAGAGTGGGCTCAATTGATTAAAGAAGCTGGATTTGGGAGCTACAAGATCTTTCCCATTATGGGTGTGAGATCTCTTATAGAGATTTACCCATAA

Protein sequence

MNMEGGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSLLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSMLEPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVLLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVEGDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYRKDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP*

Homology

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BLAST of Chy1G004660 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 1.3e-119
Identity = 216/355 (60.85%), Postives = 269/355 (75.77%), Query Frame = 0


Query: 15  ELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSLLVSSLQLHPNKTQF 74
           E+L AQ+HIWN IFNFINSMSLKCAIQLGIPD I++HG  PM +S L  +L ++  K+  
Sbjct: 25  EILGAQAHIWNQIFNFINSMSLKCAIQLGIPDIINNHG-KPMTISQLTLALPINRKKSPC 84

Query: 75  IYRLMRLLTHSGFFVQQ-----------EEGYILTNSSRLLLKDNPCAVSPFLLSMLEPA 134
           +YRLMR+L HSGFF  Q           EEGY++T++S+LLLKDNP +V+PFLL+ML+P 
Sbjct: 85  VYRLMRILIHSGFFALQKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLDPV 144

Query: 135 LTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVLLGK 194
           +T PW FLS WFQ  D TPF+TA+GM FW+Y  ++PK    FN  MASDARLV SV++ K
Sbjct: 145 MTKPWDFLSNWFQNGDPTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLVTSVVIEK 204

Query: 195 HKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVEGDM 254
            K VFEGVESLVDVGGGTGT+A +I+ AFP ++CTVFDLP VVA L+G   NLN+V GDM
Sbjct: 205 CKGVFEGVESLVDVGGGTGTVASSIAAAFPHIQCTVFDLPHVVADLQGGN-NLNFVGGDM 264

Query: 255 FKVIPPADVLLLKWILHDWSDEECVEILKNCKAAI-RSNGYKGKVMVIDIVLFGNYRKD- 314
           F  +P  +V+LLKWILHDW+DEE V+ILK CK AI +SN   GKV++ID+ +     +D 
Sbjct: 265 FVDVPATEVVLLKWILHDWNDEESVKILKKCKEAISKSNKKGGKVIIIDMKVENEKDEDD 324

Query: 315 -SMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 356
            S ETQL FDMLMM L  G+ER EKEWA+L K+AGF +YKI PI+G+RSLIE+YP
Sbjct: 325 ESYETQLFFDMLMMALVTGRERNEKEWAKLFKDAGFSNYKITPILGLRSLIEVYP 377

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BLAST of Chy1G004660 vs. ExPASy Swiss-Prot
Match: B6VJS4 (Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 2.7e-117
Identity = 207/351 (58.97%), Postives = 259/351 (73.79%), Query Frame = 0


Query: 15  ELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSLLVSSLQLHPNKTQF 74
           ELL AQ+H+WNHIFNFI SMSLKCAIQLGIPD IH+HG  PM L  LV+ L +HP ++Q 
Sbjct: 11  ELLHAQAHVWNHIFNFIKSMSLKCAIQLGIPDIIHNHG-KPMTLPELVAKLPVHPKRSQC 70

Query: 75  IYRLMRLLTHSGFFVQQ-------EEGYILTNSSRLLLKDNPCAVSPFLLSMLEPALTDP 134
           +YRLMR+L HSGF   Q       EEGY+LT++SRLLL D+  ++ P +L+ML+P LT P
Sbjct: 71  VYRLMRILVHSGFLAAQRVQQGKEEEGYVLTDASRLLLMDDSLSIRPLVLAMLDPILTKP 130

Query: 135 WQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVLLGKHKSV 194
           W +LS WFQ DD TPF TA+   FW+Y G++P+    FN  MASDARL+ SVLL + + V
Sbjct: 131 WHYLSAWFQNDDPTPFHTAYERSFWDYAGHEPQLNNSFNEAMASDARLLTSVLLKEGQGV 190

Query: 195 FEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVEGDMFKVI 254
           F G+ SLVDVGGGTG +AKAI+ AFP + CTV DL  VVA L+G + NLNY  GDMF+ I
Sbjct: 191 FAGLNSLVDVGGGTGKVAKAIANAFPHLNCTVLDLSHVVAGLQGSK-NLNYFAGDMFEAI 250

Query: 255 PPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLF---GNYRKDSME 314
           PPAD +LLKWILHDWS+EECV+ILK C+ AI S    GKV++ID+++    G+Y+  S E
Sbjct: 251 PPADAILLKWILHDWSNEECVKILKRCREAIPSKENGGKVIIIDMIMMKNQGDYK--STE 310

Query: 315 TQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 356
           TQL FDM MM    G+ER+E EW +L  +AGF  YKI PI+G+RSLIE+YP
Sbjct: 311 TQLFFDMTMMIFAPGRERDENEWEKLFLDAGFSHYKITPILGLRSLIEVYP 357

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BLAST of Chy1G004660 vs. ExPASy Swiss-Prot
Match: A0A0N9HMN6 (Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 PE=1 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 2.9e-103
Identity = 185/367 (50.41%), Postives = 252/367 (68.66%), Query Frame = 0


Query: 1   MNMEGGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSL 60
           + +  G      G+ELL AQ+HI+NHIFNFI+SM+LKCA++L IP+ +H+H P  + LS 
Sbjct: 9   LEIRNGNGYGDSGEELLAAQAHIYNHIFNFISSMALKCAVELNIPEILHNHQPKAVTLSE 68

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFV-------QQEEGYILTNSSRLLLKDNPCAVS 120
           LV +LQ+   K+  +YRL+R+L HSGFF          EEGY+ T SS+LLLK++P ++S
Sbjct: 69  LVQALQIPQAKSACLYRLLRILVHSGFFAITKIQSEGDEEGYLPTLSSKLLLKNHPMSMS 128

Query: 121 PFLLSMLEPALTDPWQFLSIWF-QTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASD 180
           P LL ++ P +  P  F S WF ++DD TPFE  HG   W+Y G  P   E+FN  M  +
Sbjct: 129 PCLLGLVNPTMVAPMHFFSDWFKRSDDMTPFEATHGASLWKYFGETPHMAEIFNEAMGCE 188

Query: 181 ARLVMSVLLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGD 240
            RL MSV+L + K   EG+ SLVDVGGGTG + +AI++AFP ++CTV DLP VV +LKG 
Sbjct: 189 TRLAMSVVLKECKGKLEGISSLVDVGGGTGNVGRAIAEAFPNVKCTVLDLPQVVGNLKGS 248

Query: 241 QPNLNYVEGDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDI 300
             NL +V GDMF+ IPPADV+ LKWILHDW+DEEC++ILK CK AI S    GK+++ID+
Sbjct: 249 N-NLEFVSGDMFQFIPPADVVFLKWILHDWNDEECIKILKRCKEAIPSKEEGGKLIIIDM 308

Query: 301 VL----FGNYRKDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVR 356
           V+     G+Y  +S ETQL +D+ +M L  G ER E EW +L   AGF SY I P++G++
Sbjct: 309 VVNDHNKGSY--ESTETQLFYDLTLMALLTGTERTETEWKKLFVAAGFTSYIISPVLGLK 368

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BLAST of Chy1G004660 vs. ExPASy Swiss-Prot
Match: Q6VMW0 (8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 9.6e-99
Identity = 182/357 (50.98%), Postives = 246/357 (68.91%), Query Frame = 0


Query: 15  ELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSLLVSSLQLHPNKTQF 74
           ELLEAQ+H+WNHI+++INSMSLKCAIQLGIPDAIH HG NP+ LS L  +L ++  K+  
Sbjct: 12  ELLEAQAHVWNHIYSYINSMSLKCAIQLGIPDAIHKHG-NPITLSQLADALNINKAKSHG 71

Query: 75  IYRLMRLLTHSGFF--------------VQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 134
           ++RLMR+L HSGFF               ++E+ Y LT +SRLLL+  P +V+PF L+M 
Sbjct: 72  LFRLMRILVHSGFFDKVKVKVKVEGEDEEEEEDAYSLTPASRLLLRSEPLSVAPFALAMS 131

Query: 135 EPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVL 194
           +P  T+ W  LS WF+ D    F+T +GM F EY     +   +FN  MA DA  V S+L
Sbjct: 132 DPVYTETWHHLSEWFRNDAVAAFDTKYGMTFPEYAVADDRLNVLFNEAMACDAGFVNSIL 191

Query: 195 LGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVE 254
             + + +F+G+ES+VDVGGGTG  AK I+ AFP MECTV DLP VV  LKG + NL++V 
Sbjct: 192 TTECREIFDGLESMVDVGGGTGATAKGIAAAFPGMECTVLDLPNVVGGLKGSE-NLSFVS 251

Query: 255 GDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAI-RSNGYKGKVMVIDIVLFGNYR 314
           GDMF  IP AD + +K+ILHDW+DEECV+ILK CK AI RSN    K+++++IV+     
Sbjct: 252 GDMFDFIPHADAIFMKFILHDWNDEECVKILKKCKEAISRSNNSCRKIILVEIVMEDEKE 311

Query: 315 -KDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 356
             ++ ET+L FDM M+ +  GKER EKEW +L  +AGF +YKI  ++G+RS+IE++P
Sbjct: 312 THEATETKLFFDMQMLAIITGKERSEKEWGKLFFDAGFTNYKITRVLGLRSVIEVFP 366

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BLAST of Chy1G004660 vs. ExPASy Swiss-Prot
Match: Q93WU3 (Chavicol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=CVOMT1 PE=1 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 1.4e-94
Identity = 179/354 (50.56%), Postives = 240/354 (67.80%), Query Frame = 0


Query: 9   LAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSLLVSSLQLH 68
           +++  ++LL+AQ+H+WNH++ F NSMSLKCAIQLGIPD +H H  +PM LS L+ ++ ++
Sbjct: 8   ISLSTEQLLQAQAHVWNHMYAFANSMSLKCAIQLGIPDILHKH-DHPMTLSQLLKAIPIN 67

Query: 69  PNKTQFIYRLMRLLTHSGFFVQ-----QEEGYILTNSSRLLLKDNPCAVSPFLLSMLEPA 128
             K+Q   RLMR L +S FF++     QE  Y LT +SRLLLK  P  V+P +  +L+P 
Sbjct: 68  KEKSQSFQRLMRALVNSNFFIEENSNNQEVCYWLTPASRLLLKGAPLTVAPLVQVVLDPT 127

Query: 129 LTDPWQFLSIWFQTDDR-TPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVLLG 188
            T+PW ++S WF+ ++  T FE A+G  FWE   NKP  G  F+  M+ D+RLV  VL  
Sbjct: 128 FTNPWHYMSEWFKHENHATQFEAANGCTFWEKLANKPSMGRFFDEAMSCDSRLVAHVLTK 187

Query: 189 KHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVEGD 248
            +K V +G+ +LVDVGGG GTMAKAI +A P M+CTV DLP VVA L+     L+Y+ GD
Sbjct: 188 DYKHVIDGIRTLVDVGGGNGTMAKAIVEAVPTMKCTVLDLPHVVAGLESTD-KLSYIGGD 247

Query: 249 MFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYRKDS 308
           MF+ IP AD +LLK+I+HDW DEE ++ILK CK A+   G  GKV++ID+V+  N+  D 
Sbjct: 248 MFQSIPSADAILLKFIIHDWDDEEGLKILKRCKDAV---GIGGKVIIIDVVVGVNHDVDE 307

Query: 309 -METQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 356
            +E QL FDM MM+    KER   EW +LI  AGF SYK+ P  GVRSLIE YP
Sbjct: 308 VLEDQLHFDMAMMSYFNAKERTMNEWEKLISAAGFTSYKLTPAFGVRSLIEAYP 356

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BLAST of Chy1G004660 vs. ExPASy TrEMBL
Match: A0A0A0K1E9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G039280 PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.0e-196
Identity = 334/355 (94.08%), Postives = 347/355 (97.75%), Query Frame = 0


Query: 1   MNMEGGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSL 60
           MNMEGG +LAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNP+PLS+
Sbjct: 26  MNMEGGNMLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSI 85

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120
           LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML
Sbjct: 86  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 145

Query: 121 EPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVL 180
           +PALTDPWQFLSIWFQTDD+TPFETAHG+PFW+Y  NKPKEGEVFNAGMASDARLV SVL
Sbjct: 146 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVNSVL 205

Query: 181 LGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVE 240
           LGKHKSVFEGV+SLVDVGGGTGTMAKAIS+AFPQMECTVFDLP VVAHL+GDQPNLNYVE
Sbjct: 206 LGKHKSVFEGVKSLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLRGDQPNLNYVE 265

Query: 241 GDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYRK 300
           GDMFK IPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNG KGKVMVIDIVLFGNY+K
Sbjct: 266 GDMFKRIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGNKGKVMVIDIVLFGNYKK 325

Query: 301 DSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 356
           DSMETQLLFDMLMMTL GGKEREEKEWA+LIKEAGFGSYKIFPIMGVRSL+EIYP
Sbjct: 326 DSMETQLLFDMLMMTLVGGKEREEKEWAKLIKEAGFGSYKIFPIMGVRSLVEIYP 380

[Close]

BLAST of Chy1G004660 vs. ExPASy TrEMBL
Match: A0A5A7UV91 (Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G00370 PE=3 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 3.9e-188
Identity = 327/356 (91.85%), Postives = 338/356 (94.94%), Query Frame = 0


Query: 1   MNME-GGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60
           MNME GGK LAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS
Sbjct: 1   MNMEAGGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60

Query: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSM 120
           LLVSSLQLHPNKTQFIYRLMRLLTHSGFFV QEEGY+LTNSSRLLLKDNPCAVSPFLLSM
Sbjct: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVLQEEGYLLTNSSRLLLKDNPCAVSPFLLSM 120

Query: 121 LEPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSV 180
           L+PALTDPWQFLSIW QTDDRTPFETAHGMPFWEY GNK K+GEVFN GMASDARLVMSV
Sbjct: 121 LQPALTDPWQFLSIWLQTDDRTPFETAHGMPFWEYMGNKAKDGEVFNEGMASDARLVMSV 180

Query: 181 LLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYV 240
           +L KHKSVFEGVESLVDVGGGTGTMAKAIS+AFPQMECTVFDLP VVAHLK DQPN  YV
Sbjct: 181 ILEKHKSVFEGVESLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLKEDQPNFKYV 240

Query: 241 EGDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYR 300
           EGDMFK+IPPADVLLLKWILHDWSDEECVEILKNCKAAI SNG +GKVMVIDIVLFGN +
Sbjct: 241 EGDMFKLIPPADVLLLKWILHDWSDEECVEILKNCKAAITSNGDRGKVMVIDIVLFGN-K 300

Query: 301 KDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 356
           KD METQLLFDMLMMTL GGKEREE++WA+LIKEAGF SYKIFPIMGVRSLIEIYP
Sbjct: 301 KDPMETQLLFDMLMMTLAGGKEREEEKWAELIKEAGFRSYKIFPIMGVRSLIEIYP 355

[Close]

BLAST of Chy1G004660 vs. ExPASy TrEMBL
Match: A0A1S3C0K5 (trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103495637 PE=3 SV=1)

HSP 1 Score: 664.8 bits (1714), Expect = 2.0e-187
Identity = 328/356 (92.13%), Postives = 338/356 (94.94%), Query Frame = 0


Query: 1   MNME-GGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60
           MNME GGK LAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS
Sbjct: 1   MNMEAGGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60

Query: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSM 120
           LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDN  AVSPFLLSM
Sbjct: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNHFAVSPFLLSM 120

Query: 121 LEPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSV 180
           L+PALTDPWQFLSIW QTDDRTPFETAHGMPFWEY GNK K+GEVFN GMASDARLVMSV
Sbjct: 121 LQPALTDPWQFLSIWLQTDDRTPFETAHGMPFWEYMGNKAKDGEVFNEGMASDARLVMSV 180

Query: 181 LLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYV 240
           +L KHKSVFEGVESLVDVGGGTGTMAKAIS+AFPQMECTVFDLP VVAHLK DQPN  YV
Sbjct: 181 ILEKHKSVFEGVESLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLKEDQPNFKYV 240

Query: 241 EGDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYR 300
           EGDMFK+IPPADVLLLKWILHDWSDEECVEILKNCKAAI SNG +GKVMVIDIVLFGN +
Sbjct: 241 EGDMFKLIPPADVLLLKWILHDWSDEECVEILKNCKAAITSNGDRGKVMVIDIVLFGN-K 300

Query: 301 KDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 356
           KDSMETQLLFDMLMMTL GGKEREE++WA+LIKEAGF SYKIFPIMGVRSLIEIYP
Sbjct: 301 KDSMETQLLFDMLMMTLAGGKEREEEKWAELIKEAGFRSYKIFPIMGVRSLIEIYP 355

[Close]

BLAST of Chy1G004660 vs. ExPASy TrEMBL
Match: A0A5D3DDN8 (Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold150G00330 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 5.7e-187
Identity = 326/356 (91.57%), Postives = 338/356 (94.94%), Query Frame = 0


Query: 1   MNME-GGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60
           MNME GGK LAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS
Sbjct: 1   MNMEAGGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60

Query: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSM 120
           LLVSSLQLHPNKTQFIYRLMRLLTHSGFFV QEEGY+LTNSSRLLLKDNPCAVSPFLLSM
Sbjct: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVLQEEGYLLTNSSRLLLKDNPCAVSPFLLSM 120

Query: 121 LEPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSV 180
           L+PALTDP+QFLSIW QTDDRTPFETAHGMPFWEY GNK K+GEVFN GMASDARLVMSV
Sbjct: 121 LQPALTDPFQFLSIWLQTDDRTPFETAHGMPFWEYMGNKAKDGEVFNEGMASDARLVMSV 180

Query: 181 LLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYV 240
           +L KHKSVFEGVESLVDVGGGTGTMAKAIS+AFPQMECTVFDLP VVAHLK DQPN  YV
Sbjct: 181 ILEKHKSVFEGVESLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLKEDQPNFKYV 240

Query: 241 EGDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYR 300
           EGDMFK+IPPADVLLLKWILHDWSDEECVEILKNCKAAI SNG +GKVMVIDIVLFGN +
Sbjct: 241 EGDMFKLIPPADVLLLKWILHDWSDEECVEILKNCKAAITSNGDRGKVMVIDIVLFGN-K 300

Query: 301 KDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 356
           KD METQLLFDMLMMTL GGKEREE++WA+LIKEAGF SYKIFPIMGVRSLIEIYP
Sbjct: 301 KDPMETQLLFDMLMMTLAGGKEREEEKWAELIKEAGFRSYKIFPIMGVRSLIEIYP 355

[Close]

BLAST of Chy1G004660 vs. ExPASy TrEMBL
Match: A0A0A0K5D5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G039790 PE=3 SV=1)

HSP 1 Score: 638.3 bits (1645), Expect = 2.0e-179
Identity = 308/357 (86.27%), Postives = 332/357 (93.00%), Query Frame = 0


Query: 1   MNMEGGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSL 60
           MNMEGGK+LAMGGDELLEAQSH+WNHIFNFINSMSLKCAIQLGIPDAIHSHGPNP+PLS+
Sbjct: 1   MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSI 60

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120
           LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML
Sbjct: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120

Query: 121 EPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVL 180
           +PALTDPWQFLSIWFQTDD+TPFETAHG+PFW+Y  NKPKEGEVFNAGMASDARLV++VL
Sbjct: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 180

Query: 181 LGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVE 240
           L KH+SVFEGVESLVDVGGGTGT+AKAIS+AFPQMECTV DLP VVAHLKGDQPN  YVE
Sbjct: 181 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 240

Query: 241 GDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNG-YKGKVMVIDIVLFGNYR 300
           GDMF +IPPAD +LLKWILHDWSDEECVEILK CK AI  +G  KGKVMVID+VLF    
Sbjct: 241 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 300

Query: 301 -KDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 356
             DS+ETQLL+DMLMM + GGKEREEKEWA+LIKEAGF +YKIFPI+ +RSLIEIYP
Sbjct: 301 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 357

[Close]

BLAST of Chy1G004660 vs. NCBI nr
Match: XP_004151089.3 (LOW QUALITY PROTEIN: trans-resveratrol di-O-methyltransferase [Cucumis sativus])

HSP 1 Score: 692 bits (1787), Expect = 1.08e-250
Identity = 335/355 (94.37%), Postives = 345/355 (97.18%), Query Frame = 0


Query: 1   MNMEGGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSL 60
           MNMEGG +LAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSL
Sbjct: 26  MNMEGGNMLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSL 85

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120
           LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML
Sbjct: 86  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 145

Query: 121 EPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVL 180
           +PALTDPWQFLSIWFQTDD+TPFETAHG  FW+Y  NKPKEGEVFNAGMASDARLV SVL
Sbjct: 146 QPALTDPWQFLSIWFQTDDQTPFETAHGXAFWKYMRNKPKEGEVFNAGMASDARLVNSVL 205

Query: 181 LGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVE 240
           LGKHKSVFEGV+SLVDVGGGTGTMAKAIS+AFPQMECTVFDLP VVAHL+GDQPNLNYVE
Sbjct: 206 LGKHKSVFEGVKSLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLRGDQPNLNYVE 265

Query: 241 GDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYRK 300
           GDMFK IPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNG KGKVMVIDIVLFGNY+K
Sbjct: 266 GDMFKRIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGNKGKVMVIDIVLFGNYKK 325

Query: 301 DSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 355
           DSMETQLLFDMLMMTL GGKEREEKEWA+LIKEAGFGSYKIFPIMGVRSL+EIYP
Sbjct: 326 DSMETQLLFDMLMMTLVGGKEREEKEWAKLIKEAGFGSYKIFPIMGVRSLVEIYP 380

[Close]

BLAST of Chy1G004660 vs. NCBI nr
Match: KAA0059128.1 (trans-resveratrol di-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 665 bits (1717), Expect = 1.98e-240
Identity = 327/356 (91.85%), Postives = 338/356 (94.94%), Query Frame = 0


Query: 1   MNME-GGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60
           MNME GGK LAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS
Sbjct: 1   MNMEAGGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60

Query: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSM 120
           LLVSSLQLHPNKTQFIYRLMRLLTHSGFFV QEEGY+LTNSSRLLLKDNPCAVSPFLLSM
Sbjct: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVLQEEGYLLTNSSRLLLKDNPCAVSPFLLSM 120

Query: 121 LEPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSV 180
           L+PALTDPWQFLSIW QTDDRTPFETAHGMPFWEY GNK K+GEVFN GMASDARLVMSV
Sbjct: 121 LQPALTDPWQFLSIWLQTDDRTPFETAHGMPFWEYMGNKAKDGEVFNEGMASDARLVMSV 180

Query: 181 LLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYV 240
           +L KHKSVFEGVESLVDVGGGTGTMAKAIS+AFPQMECTVFDLP VVAHLK DQPN  YV
Sbjct: 181 ILEKHKSVFEGVESLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLKEDQPNFKYV 240

Query: 241 EGDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYR 300
           EGDMFK+IPPADVLLLKWILHDWSDEECVEILKNCKAAI SNG +GKVMVIDIVLFGN +
Sbjct: 241 EGDMFKLIPPADVLLLKWILHDWSDEECVEILKNCKAAITSNGDRGKVMVIDIVLFGN-K 300

Query: 301 KDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 355
           KD METQLLFDMLMMTL GGKEREE++WA+LIKEAGF SYKIFPIMGVRSLIEIYP
Sbjct: 301 KDPMETQLLFDMLMMTLAGGKEREEEKWAELIKEAGFRSYKIFPIMGVRSLIEIYP 355

[Close]

BLAST of Chy1G004660 vs. NCBI nr
Match: XP_008455485.2 (PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 663 bits (1711), Expect = 1.62e-239
Identity = 328/356 (92.13%), Postives = 338/356 (94.94%), Query Frame = 0


Query: 1   MNME-GGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60
           MNME GGK LAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS
Sbjct: 1   MNMEAGGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60

Query: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSM 120
           LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDN  AVSPFLLSM
Sbjct: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNHFAVSPFLLSM 120

Query: 121 LEPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSV 180
           L+PALTDPWQFLSIW QTDDRTPFETAHGMPFWEY GNK K+GEVFN GMASDARLVMSV
Sbjct: 121 LQPALTDPWQFLSIWLQTDDRTPFETAHGMPFWEYMGNKAKDGEVFNEGMASDARLVMSV 180

Query: 181 LLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYV 240
           +L KHKSVFEGVESLVDVGGGTGTMAKAIS+AFPQMECTVFDLP VVAHLK DQPN  YV
Sbjct: 181 ILEKHKSVFEGVESLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLKEDQPNFKYV 240

Query: 241 EGDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYR 300
           EGDMFK+IPPADVLLLKWILHDWSDEECVEILKNCKAAI SNG +GKVMVIDIVLFGN +
Sbjct: 241 EGDMFKLIPPADVLLLKWILHDWSDEECVEILKNCKAAITSNGDRGKVMVIDIVLFGN-K 300

Query: 301 KDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 355
           KDSMETQLLFDMLMMTL GGKEREE++WA+LIKEAGF SYKIFPIMGVRSLIEIYP
Sbjct: 301 KDSMETQLLFDMLMMTLAGGKEREEEKWAELIKEAGFRSYKIFPIMGVRSLIEIYP 355

[Close]

BLAST of Chy1G004660 vs. NCBI nr
Match: TYK21633.1 (trans-resveratrol di-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 662 bits (1707), Expect = 6.61e-239
Identity = 326/356 (91.57%), Postives = 338/356 (94.94%), Query Frame = 0


Query: 1   MNME-GGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60
           MNME GGK LAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS
Sbjct: 1   MNMEAGGKKLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLS 60

Query: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSM 120
           LLVSSLQLHPNKTQFIYRLMRLLTHSGFFV QEEGY+LTNSSRLLLKDNPCAVSPFLLSM
Sbjct: 61  LLVSSLQLHPNKTQFIYRLMRLLTHSGFFVLQEEGYLLTNSSRLLLKDNPCAVSPFLLSM 120

Query: 121 LEPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSV 180
           L+PALTDP+QFLSIW QTDDRTPFETAHGMPFWEY GNK K+GEVFN GMASDARLVMSV
Sbjct: 121 LQPALTDPFQFLSIWLQTDDRTPFETAHGMPFWEYMGNKAKDGEVFNEGMASDARLVMSV 180

Query: 181 LLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYV 240
           +L KHKSVFEGVESLVDVGGGTGTMAKAIS+AFPQMECTVFDLP VVAHLK DQPN  YV
Sbjct: 181 ILEKHKSVFEGVESLVDVGGGTGTMAKAISQAFPQMECTVFDLPQVVAHLKEDQPNFKYV 240

Query: 241 EGDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYR 300
           EGDMFK+IPPADVLLLKWILHDWSDEECVEILKNCKAAI SNG +GKVMVIDIVLFGN +
Sbjct: 241 EGDMFKLIPPADVLLLKWILHDWSDEECVEILKNCKAAITSNGDRGKVMVIDIVLFGN-K 300

Query: 301 KDSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 355
           KD METQLLFDMLMMTL GGKEREE++WA+LIKEAGF SYKIFPIMGVRSLIEIYP
Sbjct: 301 KDPMETQLLFDMLMMTLAGGKEREEEKWAELIKEAGFRSYKIFPIMGVRSLIEIYP 355

[Close]

BLAST of Chy1G004660 vs. NCBI nr
Match: XP_004151735.2 (probable O-methyltransferase 3 [Cucumis sativus])

HSP 1 Score: 637 bits (1642), Expect = 5.72e-229
Identity = 308/357 (86.27%), Postives = 332/357 (93.00%), Query Frame = 0


Query: 1   MNMEGGKLLAMGGDELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPMPLSL 60
           MNMEGGK+LAMGGDELLEAQSH+WNHIFNFINSMSLKCAIQLGIPDAIHSHGPNP+PLS+
Sbjct: 1   MNMEGGKMLAMGGDELLEAQSHVWNHIFNFINSMSLKCAIQLGIPDAIHSHGPNPVPLSI 60

Query: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120
           LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML
Sbjct: 61  LVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQEEGYILTNSSRLLLKDNPCAVSPFLLSML 120

Query: 121 EPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVL 180
           +PALTDPWQFLSIWFQTDD+TPFETAHG+PFW+Y  NKPKEGEVFNAGMASDARLV++VL
Sbjct: 121 QPALTDPWQFLSIWFQTDDQTPFETAHGVPFWKYMRNKPKEGEVFNAGMASDARLVINVL 180

Query: 181 LGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVE 240
           L KH+SVFEGVESLVDVGGGTGT+AKAIS+AFPQMECTV DLP VVAHLKGDQPN  YVE
Sbjct: 181 LEKHRSVFEGVESLVDVGGGTGTVAKAISQAFPQMECTVLDLPQVVAHLKGDQPNFKYVE 240

Query: 241 GDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYK-GKVMVIDIVLFGNYR 300
           GDMF +IPPAD +LLKWILHDWSDEECVEILK CK AI  +G K GKVMVID+VLF    
Sbjct: 241 GDMFTLIPPADTILLKWILHDWSDEECVEILKKCKEAITGSGSKKGKVMVIDLVLFNTKN 300

Query: 301 K-DSMETQLLFDMLMMTLTGGKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIYP 355
             DS+ETQLL+DMLMM + GGKEREEKEWA+LIKEAGF +YKIFPI+ +RSLIEIYP
Sbjct: 301 DADSIETQLLYDMLMMIVPGGKEREEKEWAKLIKEAGFRAYKIFPILDLRSLIEIYP 357

[Close]

BLAST of Chy1G004660 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 218.8 bits (556), Expect = 7.1e-57
Identity = 133/363 (36.64%), Postives = 198/363 (54.55%), Query Frame = 0


Query: 14  DELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSH-GPNPMPLSLLVSSLQLHPNKT 73
           DE  +A   IW ++F F +  + KCAI L IP+AI +H    P+ L+ L S++   P+  
Sbjct: 24  DEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASPS-- 83

Query: 74  QFIYRLMRLLTHSGFFVQ------QEEGYILTNSSRLLL--KDNPCAVSPFLLSMLEPAL 133
             + R+MR L H G F +         GY+ T  SR L+  + +  +++PF+L    P +
Sbjct: 84  -HLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEM 143

Query: 134 TDPWQFLSIWFQT----DDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVL 193
             PW  LS    +        PF+  HG   W +  + P   ++ N  MA DAR V+  +
Sbjct: 144 LAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVPRV 203

Query: 194 LGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVE 253
            G    +F+GV ++VDVGGGTG     + K FP ++   FDLP V+  +      +  VE
Sbjct: 204 AGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVI-EVAEVLDGVENVE 263

Query: 254 GDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGN--- 313
           GDMF  IP  D + +KW+LHDW D++C++ILKNCK A+  N   GKV++++ V+  N   
Sbjct: 264 GDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPN--IGKVLIVESVIGENKKT 323

Query: 314 ----YRKDSME-TQLLFDMLMMTLTG-GKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLI 355
                R + +E  +L+ DM+MM  T  GKER  KEW  ++KEAGF  Y++  I  V+SLI
Sbjct: 324 MIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLI 380

[Close]

BLAST of Chy1G004660 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 187.2 bits (474), Expect = 2.3e-47
Identity = 121/357 (33.89%), Postives = 175/357 (49.02%), Query Frame = 0


Query: 14  DELLEAQSHIWNHIFNFINSMSLKCAIQLGIPDAIHSH-GPNPMPLSLLVSSLQLHPNKT 73
           DE  +A   IW ++F F +  + KCAI L IP+AI +H    P+ LS L S++   P+  
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPS-- 69

Query: 74  QFIYRLMRLLTHSGFFVQ------QEEGYILTNSSRLLLKDNPCAVSPFLLSMLEPALTD 133
             + R+MR L H G F +         GY  T  SR                        
Sbjct: 70  -HLRRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSR------------------------ 129

Query: 134 PWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVLLGKHKS 193
                        R      HG   W +  +     ++ N  MA DAR V+  + G  + 
Sbjct: 130 -------------RMMITKLHGKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQG 189

Query: 194 VFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVEGDMFKV 253
           +F+GV ++VDVGGGTG     + K FP ++   FDLP V+  +      +  VEGDMF  
Sbjct: 190 LFDGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVI-EVAQVLDGVENVEGDMFDS 249

Query: 254 IPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGYKGKVMVIDIV-------LFGNYR 313
           IP +D +++KW+LHDW D++C++ILKNCK A+  N   GKV++++ V       +    R
Sbjct: 250 IPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPN--IGKVLIVECVIGEKKNTMIAEER 309

Query: 314 KDSME-TQLLFDMLMMTLTG-GKEREEKEWAQLIKEAGFGSYKIFPIMGVRSLIEIY 355
            D +E  +L  DM+MM  T  GKER  KEW  ++ EAGF  Y++     V+SLI  Y
Sbjct: 310 DDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIAY 323

[Close]

BLAST of Chy1G004660 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 163.3 bits (412), Expect = 3.6e-40
Identity = 112/317 (35.33%), Postives = 169/317 (53.31%), Query Frame = 0


Query: 57  PLSLLVSSLQLHPNKTQFIYRLMRLLTHSGF----FVQQEEG-----YILTNSSRLLLKD 116
           P+ L   +   +P+    I RL+R L          V+ EEG     Y L    + L+KD
Sbjct: 56  PVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKD 115

Query: 117 -NPCAVSPFLLSMLEPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFN 176
            +  +++P++L+    A    W +L+   Q    + +E A+    +EY        ++FN
Sbjct: 116 EDGFSIAPYVLAGCTKAKGGVWSYLTEAIQEGGASAWERANEALIFEYMKKNENLKKIFN 175

Query: 177 AGMASDARLVMSVLLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVV 236
             M +   +VM  +L  +   FEGV   VDVGG  G+    I   +P ++   FDLP   
Sbjct: 176 ESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLP--- 235

Query: 237 AHLKGDQPNLNYVE---GDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGY 296
            H+  + P ++ VE   GDMF  IP  +V+L+KWILHDW+DE+CVEILKNCK A+     
Sbjct: 236 -HIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPET-- 295

Query: 297 KGKVMVIDIVLFGNYRKDSMETQ--LLFDMLMMTLT-GGKEREEKEWAQLIKEAGFGSYK 356
            G+++VI++++     +  + T+  L  D+ MM+LT GGKER +KE+  L KEAGF   K
Sbjct: 296 -GRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPK 355

[Close]

BLAST of Chy1G004660 vs. TAIR 10
Match: AT1G51990.2 (O-methyltransferase family protein )

HSP 1 Score: 159.1 bits (401), Expect = 6.7e-39
Identity = 111/317 (35.02%), Postives = 167/317 (52.68%), Query Frame = 0


Query: 57  PLSLLVSSLQLHPNKTQFIYRLMRLLTHSGF----FVQQEEG-----YILTNSSRLLLKD 116
           P+ L   +   +P+    I RL+R L          V+ EEG     Y L    + L+KD
Sbjct: 56  PVDLASMAAPKNPHAPMMIDRLLRFLVAYSVCTCKLVKDEEGRESRAYGLGKVGKKLIKD 115

Query: 117 -NPCAVSPFLLSMLEPALTDPWQFLSIWFQTDDRTPFETAHGMPFWEYTGNKPKEGEVFN 176
            +  +++P++L+    A    W  +    Q    + +E A+    +EY        ++FN
Sbjct: 116 EDGFSIAPYVLAGCTKAKGGVWYNVQHAIQEGGASAWERANEALIFEYMKKNENLKKIFN 175

Query: 177 AGMASDARLVMSVLLGKHKSVFEGVESLVDVGGGTGTMAKAISKAFPQMECTVFDLPPVV 236
             M +   +VM  +L  +   FEGV   VDVGG  G+    I   +P ++   FDLP   
Sbjct: 176 ESMTNHTSIVMKKILENYIG-FEGVSDFVDVGGSLGSNLAQILSKYPHIKGINFDLP--- 235

Query: 237 AHLKGDQPNLNYVE---GDMFKVIPPADVLLLKWILHDWSDEECVEILKNCKAAIRSNGY 296
            H+  + P ++ VE   GDMF  IP  +V+L+KWILHDW+DE+CVEILKNCK A+     
Sbjct: 236 -HIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILHDWNDEKCVEILKNCKKALPET-- 295

Query: 297 KGKVMVIDIVLFGNYRKDSMETQ--LLFDMLMMTLT-GGKEREEKEWAQLIKEAGFGSYK 356
            G+++VI++++     +  + T+  L  D+ MM+LT GGKER +KE+  L KEAGF   K
Sbjct: 296 -GRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGGKERTKKEFEDLAKEAGFKLPK 355

[Close]

BLAST of Chy1G004660 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 157.5 bits (397), Expect = 2.0e-38
Identity = 103/320 (32.19%), Postives = 158/320 (49.38%), Query Frame = 0


Query: 34  MSLKCAIQLGIPDAIHSHGPNPMPLSLLVSSLQLHPNKTQFIYRLMRLLTHSGFFVQQ-- 93
           M+LK A++L + + +  +G    P  +       +P     + R++RLLT          
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNR 93

Query: 94  -------EEGYILTNSSRLLLK-DNPCAVSPFLLSMLEPALTDPWQFLSIWFQTDDRTPF 153
                  E  Y L    + L K ++  +++   L   +  L + W  L      D   PF
Sbjct: 94  KLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAI-LDGGIPF 153

Query: 154 ETAHGMPFWEYTGNKPKEGEVFNAGMASDARLVMSVLLGKHKSVFEGVESLVDVGGGTGT 213
             A+GM  +EY G  P+  +VFN GM++ + + M  +L  +K  FEG+ SLVDVGGG G 
Sbjct: 154 NKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG-FEGLTSLVDVGGGIGA 213

Query: 214 MAKAISKAFPQMECTVFDLPPVVAHLKGDQPNLNYVEGDMFKVIPPADVLLLKWILHDWS 273
             K I   +P ++   FDLP V+       P + +V GDMF  +P  D + +KWI HDWS
Sbjct: 214 TLKMIVSKYPNLKGINFDLPHVIEDAP-SHPGIEHVGGDMFVSVPKGDAIFMKWICHDWS 273

Query: 274 DEECVEILKNCKAAIRSNGYKGKVMVIDIVLFGNYRKDSMETQLL-FDMLMMTLT-GGKE 333
           DE CV+ LKNC  ++  +   GKV++ + +L           Q++  D +M+    GGKE
Sbjct: 274 DEHCVKFLKNCYESLPED---GKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKE 333

Query: 334 REEKEWAQLIKEAGFGSYKI 342
           R EKE+  L K +GF   K+
Sbjct: 334 RTEKEFEALAKASGFKGIKV 347

The following BLAST results are available for this feature:

BLAST of Chy1G004660 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B0ZB57	1.3e-119	60.85	Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1	[more]
B6VJS4	2.7e-117	58.97	Trans-resveratrol di-O-methyltransferase OS=Vitis vinifera OX=29760 GN=ROMT PE=1...	[more]
A0A0N9HMN6	2.9e-103	50.41	Pluviatolide O-methyltransferase OS=Sinopodophyllum hexandrum OX=93608 GN=OMT3 P...	[more]
Q6VMW0	9.6e-99	50.98	8-hydroxyquercetin 8-O-methyltransferase OS=Mentha piperita OX=34256 GN=OMT2 PE=...	[more]
Q93WU3	1.4e-94	50.56	Chavicol O-methyltransferase OS=Ocimum basilicum OX=39350 GN=CVOMT1 PE=1 SV=1	[more]

BLAST of Chy1G004660 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K1E9	1.0e-196	94.08	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G039280 PE=3 SV=1	[more]
A0A5A7UV91	3.9e-188	91.85	Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3C0K5	2.0e-187	92.13	trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A5D3DDN8	5.7e-187	91.57	Trans-resveratrol di-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A0A0K5D5	2.0e-179	86.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G039790 PE=3 SV=1	[more]

BLAST of Chy1G004660 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004151089.3	1.08e-250	94.37	LOW QUALITY PROTEIN: trans-resveratrol di-O-methyltransferase [Cucumis sativus]	[more]
KAA0059128.1	1.98e-240	91.85	trans-resveratrol di-O-methyltransferase-like [Cucumis melo var. makuwa]	[more]
XP_008455485.2	1.62e-239	92.13	PREDICTED: trans-resveratrol di-O-methyltransferase-like [Cucumis melo]	[more]
TYK21633.1	6.61e-239	91.57	trans-resveratrol di-O-methyltransferase-like [Cucumis melo var. makuwa]	[more]
XP_004151735.2	5.72e-229	86.27	probable O-methyltransferase 3 [Cucumis sativus]	[more]

BLAST of Chy1G004660 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	7.1e-57	36.64	O-methyltransferase family protein	[more]
AT4G35150.1	2.3e-47	33.89	O-methyltransferase family protein	[more]
AT1G51990.1	3.6e-40	35.33	O-methyltransferase family protein	[more]
AT1G51990.2	6.7e-39	35.02	O-methyltransferase family protein	[more]
AT5G54160.1	2.0e-38	32.19	O-methyltransferase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 128..336 e-value: 2.3E-61 score: 206.8
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 34..83 e-value: 6.5E-17 score: 61.3
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 11..104 e-value: 1.1E-37 score: 129.7
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 105..355 e-value: 1.4E-83 score: 281.8
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 33..354 e-value: 1.0E-89 score: 298.3
None	No IPR available	PANTHER	PTHR11746:SF250	O-METHYLTRANSFERASE	coord: 12..355
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 4..355 e-value: 4.5E-121 score: 402.1
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 12..355
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 24..355 score: 80.645943
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 111..353
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 13..109

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy1G004660.1	Chy1G004660.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity