Chy1G004450 (gene) Cucumber (hystrix) v1

Overview
NameChy1G004450
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionfatty acid hydroperoxide lyase, chloroplastic
LocationchrH01: 3187425 .. 3192667 (-)
RNA-Seq ExpressionChy1G004450
SyntenyChy1G004450
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACCTCACCATGACCTCCGGCGGAATGCCTTCCATACCTTCATCTATTTCCCCCCCGCCGGTCTCTTTACCTCTCAGAAATATCCCCGGCAGCTACGGTTTGCCGCTGCTGGGATCCATCGCTGACCGACTCGATTACTACTGGTTTCAAGGACCCGATAAGTTCTTCAGGACTCGTATGGAGAAGAATCGAAGCACGGTTTTCAGAACAAATGTTCCTCCGTCTTTCCCTTTCATCTCCGCTGATCCCAGAGTAGTTGCGGTTCTGGATTGCAAGTCCTTTGCGCATCTATTCGACATGGAAATCGTGGAGAAAAATAATGTTCTGGTCGGTGATTTCATGCCCAGCATTAGTTTCACCGGAAATATGAGAGTGTGTGCTTATTTGGATACCTCGGAACCCAATCACTCCAAGGTACGATCTGGATAATTTCTTCTTCCATTCTGAGGTCAACTATTTGATTCCCATTTTCTCATTTGTTTTTAAAATGGAAATTAGGGTTCCGATTGGCATTTTGGATTTGGGGATTTTTTTTTACCCCCATTTTTAGTCCGTTCCGCGCCAAGAAAGACGTCTCACGTAACCCAGCTTTTCTCATTTTATTCCTTTTCTTCCATTTATATAAAGAAGCTTCATTATAATTAAACAACTATTACAATAATAATAATCAAGCACCACTAACTCCCCATTTCTTCTTAAAATATTTTCATCTTTTTAAATTTTGTAACTTCAAAATTCAAAATTACTTCTACTTTTAATAACCCATCATTATTCATTGTTTATAATCAAAGTTTAATTTTGTTTTTTTTTTCGACTAAATAACAATTTTAACATTTTAAAAATTAGGACTAATAAGGTTTATCTATGATAGATTAATGTTTGTAATATGGTATTAGTGATTAATTTTATGAACGATAGATTTTGACAAATTGTTTTTTATGTTCTATTTTATTAAAAGTAACTAAAATTCAACGTAGATTATTCTTATTTCTAGACAATTTTAATAAAGGTACTCAAAATATTGAATATATTTCCAGGCATATTTGGTAATATGTTTTTTGTTTTTATCAAAATTAATTTTTAAAAATAATACATTTTATTTTTGAAGAAGAGAAACAAAATCAGTTCCCAAATATGATATTTGTTTATAATAATATAAAATTGAAAAAAACAGAAAAAAGAAAAACAAAATATTGAAATGGTGACCTGAGCTTAGCTTTTATTATCCCATTTTTTCTCATAAGTGCTTTGGAATTGGAGTGGGGCCATTTCTTCTCCGTTTAAAATTCGGTTGATATTTTATTTTTAGAAGTTGAGACATTGTTTCGCAATTTAACTTTCTGTACGTACTTACACATGTCATTGTCTTGCCTTATAGAACCTTTTGGGTCATGATTCATTTCTTCTCTTTTTGAAAAGCATTAAGAATCAATATTCCCCATTTTTAACTAAAATAAGATACTTACTCATACTGCCTTCATCTTTCAACTCCTCTCATCACTCATTTCAACTTTTAATCGCTATTTGTAGAGTTATAATTATCCCTACTCTCTCTTTCTCTTATATTTAATACATAAGAAATTTCACTAATTCTTTCTACACATTCAAATTTTGTTTTTAACGAGTTCTCCTGCCTTTTCTTTTCTTTTCTTTTCCTTTTTGTTATTATAAATTATGGGTGCGATATTTGAATCATAGACTTCTTCGATCACATCTATTTGTCAGTTTAGCTAAGTATTTGGTAATTTTTTTTTTTGTTTGTTTGCTACTATAGAGGTTAAAGAAAAAGACTTTGGTTCAAATATCATTTTGGTTTCTATATTTTAATGTTGTTAATCTTTCGTTTATGTTATTTTAAATGTCCAATTTTAATACTTACGCCTATCAAGAAATATAAAGTTTATCCAATGCTTTGTTTTTTATGAAAATTATTATTATTATTAGTAAGTCAATTTCAGGTTTTTTTTTCTTTTTTTGGCTAAATTTAAGATTTAAGTACAAAAGATTAAATTGAATAAATAAAAAAAGGCTATTTTTAAACAAAAATTTATGATATATAATTTGTATTTATTTTCAAGATAAATGGTTGGTACAAAAAATATATATATATTTGTGGTGTTGGAGTTTTTGGATGTAAATATCTATTGTTCGAGAGGAGCCTTCTATTTCATACGCATTTTAAACATTGAGATATGGATATGGTAGGGAATGCAACAACTGCAGGTTTTTGTCTGTTCACAATAATTGTTTGCAAATATGTTTTTCATACTTTTGTTCGTTTTTTAAACGAAAATGAGAATTTTCCTAACACTTCCAATTGTGTTCCCACTTATTAAATGCCATCATTTCTCTCCTCTTATTAAAATTTTCAAATCATATCCATAATTACATATTCAGATACTATTTTCTTAAAATAAAGTTTACATTTTTTTTTCACAAAAAGATAATATATATGTATATATTTAAAATGGCTAATTTGTGTTTGTGCAGTCAGTTTGAAAGTTAGGATTGATACTTCTAATATTTTAATATTATTATATATCTCTCTTTTTCACTCATCCTCACCAGAGAGAGGATTCCTTAGATATTACATTAAATTCATTTCACTAATTAACTTAAGCTTTTTAGCTTTAACTTTTATGTAAGAATCAAATAAATGTGGATGAAGAAAGAAGAAAGAAGGTGGAAGAGGCCGAAATGGAGGAGATGAAAGAAGAAAGGAGGAAAATAGAAAAAATGACATGGTGATATGTTGATGTTGATGTAGAGTTGCAATGAAGAAGAGGGGAGAGAAAAAAAAAATAGAGTGGTGTGGTGAGCATGTGACGAAAGTCCCACAGTATAAGTTAAAAAGGGGAGATTGTTTTTGGTATATATTTTGATCAGGTATACATATTGAAATAAAAATAAGTATAATATCCACAAGATTAAATATAATGTAGAATTACAAAGTTGAAATTTGTTTGTTTTTCATAGATTTCTGGTATTCGAATCCTTATAGTACATTTTTCGATTTTGTGTTAAAAGTGAATCTTAAATTTGTGTTATGAAACAATGTCTTGGTCAATTACAATTTTCCATTCATAATTTTGAATGACATCGAATTTGAACTCTACAAAGTTCTTCATCGGAATTGAGTTGAATGGAATTCTCATGCAGGTAAAGAACTTCATTACAGACATTCTACGGCGGAGCTCAAGGATATGGATATCGGAGTTGGAATCGAACCTGTCGACGATGTGGGACGGCATTGAATTAGAAATGGCAAAGAACAAACAATCGGGCTTCAGAAACTTTCTCCAACCAGCTCTTTTCAACTTCTTCTCCAAAACTCTTGCCGGCGCCGACACGGCAAAATCACCGGAAGTGGCAAAATCCGGGTACATAGACGTCATAATATGGCTCGGCCTCCAGCTCGTTCCCACCATCCACATCGGCGTTCTCCAGCCCCTTGAAGAAATATTCCTTCACTCTTTCCGATTACCCTTTTTCCCCATTGCTTCCCGCTACCAAAGACTCTACGATTTCTTTCAAAAAGAAGGTACTCTCTCCTCTAAACCACTCATTATCTCTCTTTCTCTTTTTCATTTTCATTAATGATAAGTAAGCTTAATCGACCATCCATTGGTTAATGAATTGAGTATTTACCACCTTAAATGTTCGAATTTAATGTTTCTAACTTTATATATAGTATTTATAGTTTGATAAACTCCTTATAAATAATCTTTAGTATAACCGATAAAAATCAATTTTTCTGTTTATTAATGATATATACTAAGGTCAATTTCACTTTCAATTTAAGATCATAATATACATTTTTTTAACGTTCGAAGGATCTATTAAAGATAAAATTGATAGTTCAAAAGCTTATTAGATATTTTTTTATAATGAACAAGGTAATATAGATTTAAATTTTAGAGTAGAAGGACTAAAACTAAAATCCAGAGATAATTGTACCATTAGCCCAAAATTAGGGATAGAATGAAAATTGCCACTTTTTAAAATGTAATTTTTTTGTTGTTGTCATCAACGGAAATTATTGAAACGACTTTATGATGCATGCATATAGGACTCTCAACTCTGGACTCTGTTTTGCATTTGCCACACACTCCTTGCTCCTCCATTTTCAATCATGCAACTTTAAAATCTGAGAGCAGTGAGAGCAAGATCACAACCCATGCAGACTATAATCCATTTTGATAATTTCTCTTTGATATAACATAAAGAAAAAAATCTAAAAACTCAAGTTTTTGAAAAGTAGGGATATTTCTTCTTGTTCTGTTTTATTTTTTTCTCAAAATTTGTTTGGGTTACTTGTCTGAAATACACTGAGAGATGTTTGGACCCCAACAATTATAATAGTTGTTGTTTTCAACTATTCTAACATATAACAATTTCCAATATTACCATTTCAAATCTCTCTTTATTATCATGTTTAATGTTACACGGTTTACTACCAATCTCTTTCTCTCCATTATTTACTATATTCTACTCAAACTAAAACAGTCTACTCTAAACAATCTCAGACTATGATAACTAATTTAACGTTTTAAACACGTTGTAAAATTTAGGGTTTGTTGTGTTTTGGAACAGGGGCGGAAGTTGTTGGGCGAGGCGTCACCGAGTTTGGATTAACAAAGGAAGAAGCCATTCACAATCTCATCTTCACCATGGGCTTCAACGCCTACGGTGGCTTCAGTCTCTTCTTCCCGGTTCTACTCGACCGTATACTCAACGACAAAACCGGTCTACAACAAAGAATCCTCGAGGAAGTCAGGTCAAAATCCAGCTCCGGTCTGACCTTCGAGTCGGTCAAGGAGATGGACCTCATCTACTCCGTCGTCTACGAGACACTCCGGCTCGACCCCCCGGTTCCCTCCCAATACGCCAGAGCTAGAAAGGATTTCAAACTAACTTCCTACGATTCAATGTATAACATCAAGAAAGGGGAACTGCTTTGTGGGTATCAGCCGCTGGTGATGAGAGATCCGAAGGTGTTCGATGAACCGGAGACGTTTAATCCAGACCGGTTCCGAGGAGAGAAAGGGGCAGCGCTGCTGGATTATTTATTCTGGTCGAATGGGCCGCAAACGGGGACGCCGAGCGAGAAGAACAAGCAGTGCGCTGGGAAGGATTTAGTGGTGCTGACGGGGGTGGTGTTTGTGGCTTACATATTTAAAAGGTATGATTCGATTGCTGGGGAAGGAGGTTCCATTACAGCTTTTCAAAGGGCTAATTGA

mRNA sequence

ATGCACCTCACCATGACCTCCGGCGGAATGCCTTCCATACCTTCATCTATTTCCCCCCCGCCGGTCTCTTTACCTCTCAGAAATATCCCCGGCAGCTACGGTTTGCCGCTGCTGGGATCCATCGCTGACCGACTCGATTACTACTGGTTTCAAGGACCCGATAAGTTCTTCAGGACTCGTATGGAGAAGAATCGAAGCACGGTTTTCAGAACAAATGTTCCTCCGTCTTTCCCTTTCATCTCCGCTGATCCCAGAGTAGTTGCGGTTCTGGATTGCAAGTCCTTTGCGCATCTATTCGACATGGAAATCGTGGAGAAAAATAATGTTCTGGTCGGTGATTTCATGCCCAGCATTAGTTTCACCGGAAATATGAGAGTGTGTGCTTATTTGGATACCTCGGAACCCAATCACTCCAAGGTAAAGAACTTCATTACAGACATTCTACGGCGGAGCTCAAGGATATGGATATCGGAGTTGGAATCGAACCTGTCGACGATGTGGGACGGCATTGAATTAGAAATGGCAAAGAACAAACAATCGGGCTTCAGAAACTTTCTCCAACCAGCTCTTTTCAACTTCTTCTCCAAAACTCTTGCCGGCGCCGACACGGCAAAATCACCGGAAGTGGCAAAATCCGGGTACATAGACGTCATAATATGGCTCGGCCTCCAGCTCGTTCCCACCATCCACATCGGCGTTCTCCAGCCCCTTGAAGAAATATTCCTTCACTCTTTCCGATTACCCTTTTTCCCCATTGCTTCCCGCTACCAAAGACTCTACGATTTCTTTCAAAAAGAAGGGGCGGAAGTTGTTGGGCGAGGCGTCACCGAGTTTGGATTAACAAAGGAAGAAGCCATTCACAATCTCATCTTCACCATGGGCTTCAACGCCTACGGTGGCTTCAGTCTCTTCTTCCCGGTTCTACTCGACCGTATACTCAACGACAAAACCGGTCTACAACAAAGAATCCTCGAGGAAGTCAGGTCAAAATCCAGCTCCGGTCTGACCTTCGAGTCGGTCAAGGAGATGGACCTCATCTACTCCGTCGTCTACGAGACACTCCGGCTCGACCCCCCGGTTCCCTCCCAATACGCCAGAGCTAGAAAGGATTTCAAACTAACTTCCTACGATTCAATGTATAACATCAAGAAAGGGGAACTGCTTTGTGGGTATCAGCCGCTGGTGATGAGAGATCCGAAGGTGTTCGATGAACCGGAGACGTTTAATCCAGACCGGTTCCGAGGAGAGAAAGGGGCAGCGCTGCTGGATTATTTATTCTGGTCGAATGGGCCGCAAACGGGGACGCCGAGCGAGAAGAACAAGCAGTGCGCTGGGAAGGATTTAGTGGTGCTGACGGGGGTGGTGTTTGTGGCTTACATATTTAAAAGGTATGATTCGATTGCTGGGGAAGGAGGTTCCATTACAGCTTTTCAAAGGGCTAATTGA

Coding sequence (CDS)

ATGCACCTCACCATGACCTCCGGCGGAATGCCTTCCATACCTTCATCTATTTCCCCCCCGCCGGTCTCTTTACCTCTCAGAAATATCCCCGGCAGCTACGGTTTGCCGCTGCTGGGATCCATCGCTGACCGACTCGATTACTACTGGTTTCAAGGACCCGATAAGTTCTTCAGGACTCGTATGGAGAAGAATCGAAGCACGGTTTTCAGAACAAATGTTCCTCCGTCTTTCCCTTTCATCTCCGCTGATCCCAGAGTAGTTGCGGTTCTGGATTGCAAGTCCTTTGCGCATCTATTCGACATGGAAATCGTGGAGAAAAATAATGTTCTGGTCGGTGATTTCATGCCCAGCATTAGTTTCACCGGAAATATGAGAGTGTGTGCTTATTTGGATACCTCGGAACCCAATCACTCCAAGGTAAAGAACTTCATTACAGACATTCTACGGCGGAGCTCAAGGATATGGATATCGGAGTTGGAATCGAACCTGTCGACGATGTGGGACGGCATTGAATTAGAAATGGCAAAGAACAAACAATCGGGCTTCAGAAACTTTCTCCAACCAGCTCTTTTCAACTTCTTCTCCAAAACTCTTGCCGGCGCCGACACGGCAAAATCACCGGAAGTGGCAAAATCCGGGTACATAGACGTCATAATATGGCTCGGCCTCCAGCTCGTTCCCACCATCCACATCGGCGTTCTCCAGCCCCTTGAAGAAATATTCCTTCACTCTTTCCGATTACCCTTTTTCCCCATTGCTTCCCGCTACCAAAGACTCTACGATTTCTTTCAAAAAGAAGGGGCGGAAGTTGTTGGGCGAGGCGTCACCGAGTTTGGATTAACAAAGGAAGAAGCCATTCACAATCTCATCTTCACCATGGGCTTCAACGCCTACGGTGGCTTCAGTCTCTTCTTCCCGGTTCTACTCGACCGTATACTCAACGACAAAACCGGTCTACAACAAAGAATCCTCGAGGAAGTCAGGTCAAAATCCAGCTCCGGTCTGACCTTCGAGTCGGTCAAGGAGATGGACCTCATCTACTCCGTCGTCTACGAGACACTCCGGCTCGACCCCCCGGTTCCCTCCCAATACGCCAGAGCTAGAAAGGATTTCAAACTAACTTCCTACGATTCAATGTATAACATCAAGAAAGGGGAACTGCTTTGTGGGTATCAGCCGCTGGTGATGAGAGATCCGAAGGTGTTCGATGAACCGGAGACGTTTAATCCAGACCGGTTCCGAGGAGAGAAAGGGGCAGCGCTGCTGGATTATTTATTCTGGTCGAATGGGCCGCAAACGGGGACGCCGAGCGAGAAGAACAAGCAGTGCGCTGGGAAGGATTTAGTGGTGCTGACGGGGGTGGTGTTTGTGGCTTACATATTTAAAAGGTATGATTCGATTGCTGGGGAAGGAGGTTCCATTACAGCTTTTCAAAGGGCTAATTGA

Protein sequence

MHLTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRMEKNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPSQYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN*
Homology
BLAST of Chy1G004450 vs. ExPASy Swiss-Prot
Match: K4CF70 (Fatty acid hydroperoxide lyase, chloroplastic OS=Solanum lycopersicum OX=4081 GN=HPL PE=1 SV=1)

HSP 1 Score: 604.0 bits (1556), Expect = 1.5e-171
Identity = 294/477 (61.64%), Postives = 368/477 (77.15%), Query Frame = 0

Query: 10  MPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRMEKNRSTVF 69
           M S P S +P PV+LP+R+IPGSYGLPL+G IADRLDY+WFQ P+ FF  RMEK++STVF
Sbjct: 1   MNSAPLS-TPAPVTLPVRSIPGSYGLPLVGPIADRLDYFWFQKPENFFTKRMEKHKSTVF 60

Query: 70  RTNVPPSFPFI-SADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNMRVCA 129
           RTNVPP FPF  S +P VVAVLD KSF+HLFDMEIVEK NVLVGDFMPS+ +TG+MRVCA
Sbjct: 61  RTNVPPCFPFFGSVNPNVVAVLDVKSFSHLFDMEIVEKANVLVGDFMPSVVYTGDMRVCA 120

Query: 130 YLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRNFLQP 189
           YLDTSEP H+++KNF  DIL+R S+ W+  L   L TM+   E +++K+  +     LQ 
Sbjct: 121 YLDTSEPKHAQIKNFSQDILKRGSKTWVPTLLKELDTMFTTFEADLSKSNTASLLPALQK 180

Query: 190 ALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHSFRLP 249
            LFNFFS T+ GAD + SPE+A SGYI +  WL +QL PT+ IGVLQPLEEI +HSF  P
Sbjct: 181 FLFNFFSLTILGADPSVSPEIANSGYIFLDSWLAIQLAPTVSIGVLQPLEEILVHSFAYP 240

Query: 250 FFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLFFPVL 309
           FF +   Y++L  F + E  EV+ R  TEF LT++EAIHNL+F +GFNA+GGFS+F P L
Sbjct: 241 FFLVKGNYEKLVQFVKNEAKEVLSRAQTEFQLTEQEAIHNLLFILGFNAFGGFSIFLPTL 300

Query: 310 LDRILNDKTG-LQQRILEEVRSK---SSSGLTFESVKEMDLIYSVVYETLRLDPPVPSQY 369
           L  + ++K   +Q+++ +EVR K   +   L+FESVKEM+L+ S VYETLRL PPVPSQY
Sbjct: 301 LGNLGDEKNADMQEKLRKEVRDKVGVNPENLSFESVKEMELVQSFVYETLRLSPPVPSQY 360

Query: 370 ARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALLDY 429
           ARARKDFKL+S+DS+Y IKKGELLCGYQPLVM+DPKVFDEPE F  +RF  EKG  LL+Y
Sbjct: 361 ARARKDFKLSSHDSVYEIKKGELLCGYQPLVMKDPKVFDEPEKFVLERFTKEKGKELLNY 420

Query: 430 LFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN 482
           LFWSNGPQTG P+E NKQCA KD+V LT  + VAYIF++YDS++   GS+T+ ++A+
Sbjct: 421 LFWSNGPQTGRPTESNKQCAAKDMVTLTASLIVAYIFQKYDSVSFSSGSLTSVKKAS 476

BLAST of Chy1G004450 vs. ExPASy Swiss-Prot
Match: Q9ZSY9 (Linolenate hydroperoxide lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74B2 PE=1 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 3.6e-157
Identity = 262/465 (56.34%), Postives = 348/465 (74.84%), Query Frame = 0

Query: 20  PPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRMEKNRSTVFRTNVPPSFPF 79
           PP  LPLR +PGSYG PL+G ++DRLDY+WFQGPDKFFRTR EK +STVFRTN+PP+FPF
Sbjct: 27  PPSQLPLRTMPGSYGWPLVGPLSDRLDYFWFQGPDKFFRTRAEKYKSTVFRTNIPPTFPF 86

Query: 80  I-SADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNMRVCAYLDTSEPNHS 139
             + +P +VAVLD KSF+HLFDM++V+K +VL+GDF PS+ F G + V   LDT+EP H+
Sbjct: 87  FGNVNPNIVAVLDVKSFSHLFDMDLVDKRDVLIGDFRPSLGFYGGVCVGVNLDTTEPKHA 146

Query: 140 KVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRNFLQPALFNFFSKTL 199
           K+K F  + L+RSS++W+ EL SNL+  W  IE E++KN  + +   LQ  +F+F   +L
Sbjct: 147 KIKGFAMETLKRSSKVWLQELRSNLNIFWGTIESEISKNGAASYIFPLQRCIFSFLCASL 206

Query: 200 AGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVL-QPLEEIFLHSFRLPFFPIASRYQ 259
           AG D + SP++A++G+  +  WL LQ++PT  +GV+ QPLEEI LH++  P   IA  Y+
Sbjct: 207 AGVDASVSPDIAENGWKTINTWLALQVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYK 266

Query: 260 RLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLFFPVLLDRILNDKT 319
           +LY+F  +   + +  G  EF LT++EAI NL+F +GFNAYGGFS+F P L+ RI  D +
Sbjct: 267 KLYNFIDENAGDCLRLGQEEFRLTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNS 326

Query: 320 GLQQRILEEVRSKSSSG--LTFESVKEMDLIYSVVYETLRLDPPVPSQYARARKDFKLTS 379
           GLQ+RI  EVR    SG  L F++V EM+L+ SVVYETLR +PPVP Q+ARARKDF+++S
Sbjct: 327 GLQERIRTEVRRVCGSGSDLNFKTVNEMELVKSVVYETLRFNPPVPLQFARARKDFQISS 386

Query: 380 YDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALLDYLFWSNGPQTGT 439
           +D+++ +KKGELLCGYQPLVMRD  VFDEPE F PDR+ GE G+ LL+YL+WSNGPQTGT
Sbjct: 387 HDAVFEVKKGELLCGYQPLVMRDANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGT 446

Query: 440 PSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRA 481
           PS  NKQCA KD+V LT  + VA +F RYD+I G+ GSI A  +A
Sbjct: 447 PSASNKQCAAKDIVTLTASLLVADLFLRYDTITGDSGSIKAVVKA 491

BLAST of Chy1G004450 vs. ExPASy Swiss-Prot
Match: B3LF83 (Probable inactive linolenate hydroperoxide lyase OS=Arabidopsis thaliana OX=3702 GN=CYP74B2 PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 1.1e-121
Identity = 207/383 (54.05%), Postives = 280/383 (73.11%), Query Frame = 0

Query: 101 MEIVEKNNVLVGDFMPSISFTGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELE 160
           M++V+K +VL+GDF PS+ F G +RV  YLDT+EP H+K+K F  + L+RSS++W+ EL 
Sbjct: 1   MDLVDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELR 60

Query: 161 SNLSTMWDGIELEMAKNKQSGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIW 220
           SNL+  W  IE E++KN  + +   LQ  +F+F   +LAG D + SP++A++G+  +  W
Sbjct: 61  SNLNIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTW 120

Query: 221 LGLQLVPTIHIGVL-QPLEEIFLHSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFG 280
           L LQ++PT  +GV+ QPLEEI LH++  P   IA  Y++LY+F  +   + +  G  EFG
Sbjct: 121 LALQVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFG 180

Query: 281 LTKEEAIHNLIFTMGFNAYGGFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSG--LTF 340
           LT++EAI NL+F +GFNAYGGFS+F P L+ RI  D +GLQ+RI  EVR    SG  L F
Sbjct: 181 LTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNF 240

Query: 341 ESVKEMDLIYSVVYETLRLDPPVPSQYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMR 400
           ++V EM+L+ SVVYETLR  PPVP Q+ARARKDF+++S+D+++ +KKGELLCGYQPLVMR
Sbjct: 241 KTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMR 300

Query: 401 DPKVFDEPETFNPDRFRGEKGAALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFV 460
           D  VFDEPE F PDR+ GE G+ LL+YL+WSNGPQTGTPS  NKQCA KD+V LT  + V
Sbjct: 301 DANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLV 360

Query: 461 AYIFKRYDSIAGEGGSITAFQRA 481
           A +F RYD+I G+ GSI A  +A
Sbjct: 361 ADLFLRYDTITGDSGSIKAVVKA 383

BLAST of Chy1G004450 vs. ExPASy Swiss-Prot
Match: P48417 (Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.3e-109
Identity = 201/470 (42.77%), Postives = 296/470 (62.98%), Query Frame = 0

Query: 13  IPSSISPPPVS-----LPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRMEKNRST 72
           IP   S PP S     LP+R IPG YGLP +G I DRLDY++ QG ++FF++R++K +ST
Sbjct: 54  IPGITSQPPPSSDETTLPIRQIPGDYGLPGIGPIQDRLDYFYNQGREEFFKSRLQKYKST 113

Query: 73  VFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNMRVC 132
           V+R N+PP  PFI+++PRV+ +LD KSF  LFDM  VEK ++  G +MPS   TG  R+ 
Sbjct: 114 VYRANMPPG-PFIASNPRVIVLLDAKSFPVLFDMSKVEKKDLFTGTYMPSTELTGGYRIL 173

Query: 133 AYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRNFLQ 192
           +YLD SEPNH+K+K  + ++++      I E  S+ + + + +E ++A   ++ F +  +
Sbjct: 174 SYLDPSEPNHTKLKQLLFNLIKNRRDYVIPEFSSSFTDLCEVVEYDLATKGKAAFNDPAE 233

Query: 193 PALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHSFRL 252
            A FNF S+   G     +P + K     +  W+   L P + +G+ + +EE  LHS RL
Sbjct: 234 QAAFNFLSRAFFGVKPIDTP-LGKDAPSLISKWVLFNLAPILSVGLPKEVEEATLHSVRL 293

Query: 253 PFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLFFPV 312
           P   + + Y RLY+FF      V+     + G++++EA HN++F + FN++GGF + FP 
Sbjct: 294 PPLLVQNDYHRLYEFFTSAAGSVLDE-AEQSGISRDEACHNILFAVCFNSWGGFKILFPS 353

Query: 313 LLDRI----LNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPSQ 372
           L+  I    L   T L Q I   ++S     +T  ++++M L+ SVVYETLR++PPV  Q
Sbjct: 354 LMKWIGRAGLELHTKLAQEIRSAIQSTGGGKVTMAAMEQMPLMKSVVYETLRIEPPVALQ 413

Query: 373 YARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALLD 432
           Y +A+KDF L S+++ Y +K+GE+L GYQP   +DPK+FD PE F  DRF GE G  L++
Sbjct: 414 YGKAKKDFILESHEAAYQVKEGEMLFGYQPFATKDPKIFDRPEEFVADRFVGE-GVKLME 473

Query: 433 YLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGS 474
           Y+ WSNGP+T TPS  NKQCAGKD VV+   +FV  +FKRYDS   E G+
Sbjct: 474 YVMWSNGPETETPSVANKQCAGKDFVVMAARLFVVELFKRYDSFDIEVGT 519

BLAST of Chy1G004450 vs. ExPASy Swiss-Prot
Match: Q40778 (Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2)

HSP 1 Score: 396.0 bits (1016), Expect = 6.2e-109
Identity = 200/457 (43.76%), Postives = 296/457 (64.77%), Query Frame = 0

Query: 21  PVSLPLRNIPGSYGLPLLGSIADRLDY-YWFQGPDKFFRTRMEKNRSTVFRTNVPPSFPF 80
           P S PLR IPGSYG+P    I DRL+Y Y   G D++FR+RM+K +STVFR N+PP  PF
Sbjct: 3   PSSKPLREIPGSYGIPFFQPIKDRLEYFYGTGGRDEYFRSRMQKYQSTVFRANMPPG-PF 62

Query: 81  ISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNMRVCAYLDTSEPNHSK 140
           +S++P+V+ +LD KSF  LFD+  VEK ++  G +MPS   TG  RV +YLD SEP H++
Sbjct: 63  VSSNPKVIVLLDAKSFPILFDVSKVEKKDLFTGTYMPSTKLTGGYRVLSYLDPSEPRHAQ 122

Query: 141 VKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRNFLQPALFNFFSKTLA 200
           +KN +  +L+ SS   I + E+  + +++G+E E+AKN ++ F +  + A F F  +   
Sbjct: 123 LKNLLFFMLKNSSNRVIPQFETTYTELFEGLEAELAKNGKAAFNDVGEQAAFRFLGRAYF 182

Query: 201 GADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHSFRLPFFPIASRYQRL 260
            ++  ++ ++  S    +  W+   L PT+ +G+   L+E  LH+FRLP F I S Y +L
Sbjct: 183 NSNPEET-KLGTSAPTLISSWVLFNLAPTLDLGLPWFLQEPLLHTFRLPAFLIKSTYNKL 242

Query: 261 YDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLFFPVLLDRILNDKTGL 320
           YD+FQ     V+ +   + G+ K+EA+HN++F + FN +GG  + FP  L  I      L
Sbjct: 243 YDYFQSVATPVMEQ-AEKLGVPKDEAVHNILFAVCFNTFGGVKILFPNTLKWIGLAGENL 302

Query: 321 QQRILEEVR----SKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPSQYARARKDFKLTS 380
             ++ EE+R    S     +T E++++M L  SVVYE+LR++PPVP QY +A+ +F + S
Sbjct: 303 HTQLAEEIRGAIKSYGDGNVTLEAIEQMPLTKSVVYESLRIEPPVPPQYGKAKSNFTIES 362

Query: 381 YDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALLDYLFWSNGPQTGT 440
           +D+ + +KKGE+L GYQP   +DPKVFD PE + PDRF G+ G ALL Y++WSNGP+T +
Sbjct: 363 HDATFEVKKGEMLFGYQPFATKDPKVFDRPEEYVPDRFVGD-GEALLKYVWWSNGPETES 422

Query: 441 PSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGG 473
           P+ +NKQCAGKD VVL   +FV  +F+RYDS   E G
Sbjct: 423 PTVENKQCAGKDFVVLITRLFVIELFRRYDSFEIELG 455

BLAST of Chy1G004450 vs. ExPASy TrEMBL
Match: A0A0A0K5I0 (13-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G041960 PE=4 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.3e-274
Identity = 469/477 (98.32%), Postives = 473/477 (99.16%), Query Frame = 0

Query: 5   MTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRMEKN 64
           MTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSI DRLDYYWFQGPDKFFRTRMEKN
Sbjct: 1   MTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIGDRLDYYWFQGPDKFFRTRMEKN 60

Query: 65  RSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNM 124
           RSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNM
Sbjct: 61  RSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNM 120

Query: 125 RVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRN 184
           RVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRN
Sbjct: 121 RVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRN 180

Query: 185 FLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHS 244
           FLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHS
Sbjct: 181 FLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHS 240

Query: 245 FRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLF 304
           FRLPFFPIASRYQRLYDFFQKEGAEVV RGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLF
Sbjct: 241 FRLPFFPIASRYQRLYDFFQKEGAEVVERGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLF 300

Query: 305 FPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPSQY 364
           FPVLLDRILNDKTGLQQRIL+EVRSKSSSGLTFESVKEMDLIYS+VYETLRLDPPVPSQY
Sbjct: 301 FPVLLDRILNDKTGLQQRILKEVRSKSSSGLTFESVKEMDLIYSIVYETLRLDPPVPSQY 360

Query: 365 ARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALLDY 424
           ARARKDFKLTSY+S YNIKKGELLCGYQPLVMRDP+VFDEPE FNPDRFRGEKGAALLDY
Sbjct: 361 ARARKDFKLTSYNSTYNIKKGELLCGYQPLVMRDPEVFDEPEAFNPDRFRGEKGAALLDY 420

Query: 425 LFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN 482
           LFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN
Sbjct: 421 LFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN 477

BLAST of Chy1G004450 vs. ExPASy TrEMBL
Match: A0A1S4E196 (fatty acid hydroperoxide lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103495615 PE=4 SV=1)

HSP 1 Score: 936.4 bits (2419), Expect = 4.7e-269
Identity = 461/482 (95.64%), Postives = 466/482 (96.68%), Query Frame = 0

Query: 1   MHLTMT-SGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRT 60
           MHLTMT SGGMPSIPSSISPPPVSLPLRNIPGSYG PLLGSI+DRLDYYWFQGPDKFFRT
Sbjct: 1   MHLTMTSSGGMPSIPSSISPPPVSLPLRNIPGSYGFPLLGSISDRLDYYWFQGPDKFFRT 60

Query: 61  RMEKNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSIS 120
           RMEKNRSTVFRTNVPPSFPF   DPRV+AVLDCKSFAHLFDMEIVEK NVLVGDFMPSIS
Sbjct: 61  RMEKNRSTVFRTNVPPSFPFFPTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSIS 120

Query: 121 FTGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQ 180
           FTGNMRVCAYLDTSE  HSK+KNFITDILRRSSRIWISELESNLSTMWDGIE E+AKNK 
Sbjct: 121 FTGNMRVCAYLDTSESQHSKIKNFITDILRRSSRIWISELESNLSTMWDGIESEIAKNKT 180

Query: 181 SGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEE 240
           SGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEE
Sbjct: 181 SGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEE 240

Query: 241 IFLHSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYG 300
           IFLHSFRLPFFPIASRYQRLYDFFQKEG EVV RGVTEFGLTKEEAIHNLIFTMGFNAYG
Sbjct: 241 IFLHSFRLPFFPIASRYQRLYDFFQKEGKEVVERGVTEFGLTKEEAIHNLIFTMGFNAYG 300

Query: 301 GFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPP 360
           GFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYS+VYETLRLDPP
Sbjct: 301 GFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSLVYETLRLDPP 360

Query: 361 VPSQYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGA 420
           VPSQYARARKDFKLTSYDS YNIKKGELLCGYQPLVMRDPKVFDEPE FNPDRFRGEKG 
Sbjct: 361 VPSQYARARKDFKLTSYDSAYNIKKGELLCGYQPLVMRDPKVFDEPEAFNPDRFRGEKGV 420

Query: 421 ALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQR 480
           ALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLT VVFVAYIFKRYDSIAGEGGSITAFQR
Sbjct: 421 ALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTAVVFVAYIFKRYDSIAGEGGSITAFQR 480

Query: 481 AN 482
           AN
Sbjct: 481 AN 482

BLAST of Chy1G004450 vs. ExPASy TrEMBL
Match: Q66UT1 (13-hydroperoxide lyase OS=Citrullus lanatus OX=3654 PE=2 SV=1)

HSP 1 Score: 881.3 bits (2276), Expect = 1.8e-252
Identity = 426/481 (88.57%), Postives = 452/481 (93.97%), Query Frame = 0

Query: 1   MHLTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTR 60
           M +TMTSGGMPSIPSSISPPPV+LPLRNIPGSYGLPL GSI DRLDY+WFQGP+KFFR+R
Sbjct: 1   MKVTMTSGGMPSIPSSISPPPVTLPLRNIPGSYGLPLFGSIGDRLDYFWFQGPEKFFRSR 60

Query: 61  MEKNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISF 120
           MEKN+STVFRTNVPPSFPF   DPRV+AVLDCKSFAHLFDMEIVEK NVLVGDFMPS SF
Sbjct: 61  MEKNQSTVFRTNVPPSFPFFFTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 120

Query: 121 TGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQS 180
           TGNMRVCAYLDTSE  HSK+KNF+ D+LRRSSRIWI ELESNLSTMWD IE E+AK+ +S
Sbjct: 121 TGNMRVCAYLDTSESQHSKIKNFVMDVLRRSSRIWIQELESNLSTMWDSIESEIAKDTKS 180

Query: 181 GFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEI 240
            FRN LQP LFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIG+LQPLEEI
Sbjct: 181 SFRNHLQPTLFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGILQPLEEI 240

Query: 241 FLHSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGG 300
           FLHSFRLPFFPIASRYQRLYDF QKEG EVV RGV+EFGLTK+EAIHNLIFTMGFNAYGG
Sbjct: 241 FLHSFRLPFFPIASRYQRLYDFIQKEGEEVVERGVSEFGLTKDEAIHNLIFTMGFNAYGG 300

Query: 301 FSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPV 360
           FSLFFPVLLDRILNDKTGLQQRILEEV++K+ SGLTFESVKEMDLIYSVVYETLRLDPPV
Sbjct: 301 FSLFFPVLLDRILNDKTGLQQRILEEVKAKTGSGLTFESVKEMDLIYSVVYETLRLDPPV 360

Query: 361 PSQYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAA 420
           P+QYARARKDFKL+SYDS Y+IKKGELLCGYQPLVMRDPKVF++P+TFNP RFRGEKGAA
Sbjct: 361 PTQYARARKDFKLSSYDSAYSIKKGELLCGYQPLVMRDPKVFNKPKTFNPGRFRGEKGAA 420

Query: 421 LLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRA 480
           LLDYLFWSNGPQTG PSE NKQCAGKDLVVLT VVFVAYIF+RYD IAGEGGSITAFQR 
Sbjct: 421 LLDYLFWSNGPQTGLPSEHNKQCAGKDLVVLTAVVFVAYIFRRYDWIAGEGGSITAFQRT 480

Query: 481 N 482
           N
Sbjct: 481 N 481

BLAST of Chy1G004450 vs. ExPASy TrEMBL
Match: A0A6J1HZX6 (fatty acid hydroperoxide lyase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111468182 PE=4 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 1.1e-238
Identity = 399/479 (83.30%), Postives = 438/479 (91.44%), Query Frame = 0

Query: 3   LTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRME 62
           + M S G+PSIPSSISPPPV+LPL+NIPGS+GLPL GSIADRLDY+WFQGPDKFFR+RME
Sbjct: 2   VAMASSGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFRSRME 61

Query: 63  KNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTG 122
           KNRSTVFRTNVPPSFPFI +DP+V AVLDCKSFAHLFDME+VEK NVLVGDFMPS SFTG
Sbjct: 62  KNRSTVFRTNVPPSFPFILSDPKVTAVLDCKSFAHLFDMEVVEKKNVLVGDFMPSTSFTG 121

Query: 123 NMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGF 182
           NMRVCAYLDTSE  HSK+KNF  DILRRSS+IWI ELESNLST WDGIE E+A N++S F
Sbjct: 122 NMRVCAYLDTSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQKSSF 181

Query: 183 RNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFL 242
           RN LQPALFNFFSKTL GADTAKSPEVAKS +IDVIIWLGLQLVPTIH+G+ QPLEEIFL
Sbjct: 182 RNLLQPALFNFFSKTLVGADTAKSPEVAKSAHIDVIIWLGLQLVPTIHLGIFQPLEEIFL 241

Query: 243 HSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFS 302
           HSFRLPFFPIA RY+RLYDF +KEG EVV RGV+EFGL K+EAIHNLIFT+GFNAYGGFS
Sbjct: 242 HSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKDEAIHNLIFTLGFNAYGGFS 301

Query: 303 LFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPS 362
           LF PVL+DRILND+TGLQQRI++EVR+KS SGLTFESVKEM+L+YS VYETLRLDPPVP+
Sbjct: 302 LFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSFVYETLRLDPPVPT 361

Query: 363 QYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALL 422
           QYARARKDFKL+S+D++++IKKGELLCGYQPLVMRDP VF+EPE F PDRFR EKGAALL
Sbjct: 362 QYARARKDFKLSSHDAVFDIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEKGAALL 421

Query: 423 DYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN 482
           DYLFWSNGPQTG  SE NKQCA KDLVVLT VVFVAYIF+RYDSIAGEGGSIT  QR N
Sbjct: 422 DYLFWSNGPQTGMASEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITTLQRPN 480

BLAST of Chy1G004450 vs. ExPASy TrEMBL
Match: A0A6J1GJZ6 (fatty acid hydroperoxide lyase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455056 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.3e-238
Identity = 397/479 (82.88%), Postives = 440/479 (91.86%), Query Frame = 0

Query: 3   LTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRME 62
           + M SGG+PSIPSSISPPPV+LPL+NIPGS+GLPL GSIADRLDY+WFQGPDKFFR+R+E
Sbjct: 56  VAMASGGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFRSRIE 115

Query: 63  KNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTG 122
           KNRSTVFRTNVPPSFPF  +DP+V+AVLDCKSFAHLFDME+VEK NVLVGDFMPS SFTG
Sbjct: 116 KNRSTVFRTNVPPSFPFFLSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMPSTSFTG 175

Query: 123 NMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGF 182
           NMR+CAYLD SE  HSK+KNF  DILRRSS+IWI ELESNLST WDGIE E+A N++S +
Sbjct: 176 NMRMCAYLDPSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQKSTY 235

Query: 183 RNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFL 242
           RN LQPALFNFFSKTLAGADTAKSPEVAKS YIDVIIWLGLQLVPTIH+G+LQPLEEIFL
Sbjct: 236 RNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGILQPLEEIFL 295

Query: 243 HSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFS 302
           HSFRLPFFPIA RY+RLYDF +KEG EVV RGV+EFGL KEEAIHNLIFT+GFNAYGGFS
Sbjct: 296 HSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFTLGFNAYGGFS 355

Query: 303 LFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPS 362
           LF PVL+DRILND+TGLQQRI++EVR+KS SGLTFESVKEM+L+YS+VYE LRLDPPVP+
Sbjct: 356 LFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYEALRLDPPVPT 415

Query: 363 QYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALL 422
           QYARARK+FKL+S+D+++ IKKGELLCGYQPLVMRDP VF+EPE F PDRFR EKGAALL
Sbjct: 416 QYARARKEFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEKGAALL 475

Query: 423 DYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN 482
           DYLFWSNGPQTG  SE NKQCA KDLVVLT VVFVAYIF+RYDSIAGEGGSITA Q+ N
Sbjct: 476 DYLFWSNGPQTGMSSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITALQKPN 534

BLAST of Chy1G004450 vs. NCBI nr
Match: XP_004144503.1 (fatty acid hydroperoxide lyase, chloroplastic [Cucumis sativus] >KAE8645736.1 hypothetical protein Csa_020415 [Cucumis sativus])

HSP 1 Score: 956 bits (2472), Expect = 0.0
Identity = 473/481 (98.34%), Postives = 477/481 (99.17%), Query Frame = 0

Query: 1   MHLTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTR 60
           MHLTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSI DRLDYYWFQGPDKFFRTR
Sbjct: 1   MHLTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIGDRLDYYWFQGPDKFFRTR 60

Query: 61  MEKNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISF 120
           MEKNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISF
Sbjct: 61  MEKNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISF 120

Query: 121 TGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQS 180
           TGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQS
Sbjct: 121 TGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQS 180

Query: 181 GFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEI 240
           GFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEI
Sbjct: 181 GFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEI 240

Query: 241 FLHSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGG 300
           FLHSFRLPFFPIASRYQRLYDFFQKEGAEVV RGVTEFGLTKEEAIHNLIFTMGFNAYGG
Sbjct: 241 FLHSFRLPFFPIASRYQRLYDFFQKEGAEVVERGVTEFGLTKEEAIHNLIFTMGFNAYGG 300

Query: 301 FSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPV 360
           FSLFFPVLLDRILNDKTGLQQRIL+EVRSKSSSGLTFESVKEMDLIYS+VYETLRLDPPV
Sbjct: 301 FSLFFPVLLDRILNDKTGLQQRILKEVRSKSSSGLTFESVKEMDLIYSIVYETLRLDPPV 360

Query: 361 PSQYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAA 420
           PSQYARARKDFKLTSY+S YNIKKGELLCGYQPLVMRDP+VFDEPE FNPDRFRGEKGAA
Sbjct: 361 PSQYARARKDFKLTSYNSTYNIKKGELLCGYQPLVMRDPEVFDEPEAFNPDRFRGEKGAA 420

Query: 421 LLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRA 480
           LLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRA
Sbjct: 421 LLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRA 480

BLAST of Chy1G004450 vs. NCBI nr
Match: XP_016901760.1 (PREDICTED: fatty acid hydroperoxide lyase, chloroplastic [Cucumis melo])

HSP 1 Score: 929 bits (2402), Expect = 0.0
Identity = 461/482 (95.64%), Postives = 466/482 (96.68%), Query Frame = 0

Query: 1   MHLTMTS-GGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRT 60
           MHLTMTS GGMPSIPSSISPPPVSLPLRNIPGSYG PLLGSI+DRLDYYWFQGPDKFFRT
Sbjct: 1   MHLTMTSSGGMPSIPSSISPPPVSLPLRNIPGSYGFPLLGSISDRLDYYWFQGPDKFFRT 60

Query: 61  RMEKNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSIS 120
           RMEKNRSTVFRTNVPPSFPF   DPRV+AVLDCKSFAHLFDMEIVEK NVLVGDFMPSIS
Sbjct: 61  RMEKNRSTVFRTNVPPSFPFFPTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSIS 120

Query: 121 FTGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQ 180
           FTGNMRVCAYLDTSE  HSK+KNFITDILRRSSRIWISELESNLSTMWDGIE E+AKNK 
Sbjct: 121 FTGNMRVCAYLDTSESQHSKIKNFITDILRRSSRIWISELESNLSTMWDGIESEIAKNKT 180

Query: 181 SGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEE 240
           SGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEE
Sbjct: 181 SGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEE 240

Query: 241 IFLHSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYG 300
           IFLHSFRLPFFPIASRYQRLYDFFQKEG EVV RGVTEFGLTKEEAIHNLIFTMGFNAYG
Sbjct: 241 IFLHSFRLPFFPIASRYQRLYDFFQKEGKEVVERGVTEFGLTKEEAIHNLIFTMGFNAYG 300

Query: 301 GFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPP 360
           GFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYS+VYETLRLDPP
Sbjct: 301 GFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSLVYETLRLDPP 360

Query: 361 VPSQYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGA 420
           VPSQYARARKDFKLTSYDS YNIKKGELLCGYQPLVMRDPKVFDEPE FNPDRFRGEKG 
Sbjct: 361 VPSQYARARKDFKLTSYDSAYNIKKGELLCGYQPLVMRDPKVFDEPEAFNPDRFRGEKGV 420

Query: 421 ALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQR 480
           ALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLT VVFVAYIFKRYDSIAGEGGSITAFQR
Sbjct: 421 ALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTAVVFVAYIFKRYDSIAGEGGSITAFQR 480

BLAST of Chy1G004450 vs. NCBI nr
Match: AAU12570.1 (13-hydroperoxide lyase [Citrullus lanatus])

HSP 1 Score: 874 bits (2259), Expect = 0.0
Identity = 426/481 (88.57%), Postives = 452/481 (93.97%), Query Frame = 0

Query: 1   MHLTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTR 60
           M +TMTSGGMPSIPSSISPPPV+LPLRNIPGSYGLPL GSI DRLDY+WFQGP+KFFR+R
Sbjct: 1   MKVTMTSGGMPSIPSSISPPPVTLPLRNIPGSYGLPLFGSIGDRLDYFWFQGPEKFFRSR 60

Query: 61  MEKNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISF 120
           MEKN+STVFRTNVPPSFPF   DPRV+AVLDCKSFAHLFDMEIVEK NVLVGDFMPS SF
Sbjct: 61  MEKNQSTVFRTNVPPSFPFFFTDPRVIAVLDCKSFAHLFDMEIVEKKNVLVGDFMPSTSF 120

Query: 121 TGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQS 180
           TGNMRVCAYLDTSE  HSK+KNF+ D+LRRSSRIWI ELESNLSTMWD IE E+AK+ +S
Sbjct: 121 TGNMRVCAYLDTSESQHSKIKNFVMDVLRRSSRIWIQELESNLSTMWDSIESEIAKDTKS 180

Query: 181 GFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEI 240
            FRN LQP LFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIG+LQPLEEI
Sbjct: 181 SFRNHLQPTLFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGILQPLEEI 240

Query: 241 FLHSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGG 300
           FLHSFRLPFFPIASRYQRLYDF QKEG EVV RGV+EFGLTK+EAIHNLIFTMGFNAYGG
Sbjct: 241 FLHSFRLPFFPIASRYQRLYDFIQKEGEEVVERGVSEFGLTKDEAIHNLIFTMGFNAYGG 300

Query: 301 FSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPV 360
           FSLFFPVLLDRILNDKTGLQQRILEEV++K+ SGLTFESVKEMDLIYSVVYETLRLDPPV
Sbjct: 301 FSLFFPVLLDRILNDKTGLQQRILEEVKAKTGSGLTFESVKEMDLIYSVVYETLRLDPPV 360

Query: 361 PSQYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAA 420
           P+QYARARKDFKL+SYDS Y+IKKGELLCGYQPLVMRDPKVF++P+TFNP RFRGEKGAA
Sbjct: 361 PTQYARARKDFKLSSYDSAYSIKKGELLCGYQPLVMRDPKVFNKPKTFNPGRFRGEKGAA 420

Query: 421 LLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRA 480
           LLDYLFWSNGPQTG PSE NKQCAGKDLVVLT VVFVAYIF+RYD IAGEGGSITAFQR 
Sbjct: 421 LLDYLFWSNGPQTGLPSEHNKQCAGKDLVVLTAVVFVAYIFRRYDWIAGEGGSITAFQRT 480

BLAST of Chy1G004450 vs. NCBI nr
Match: XP_023554457.1 (fatty acid hydroperoxide lyase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 830 bits (2145), Expect = 2.29e-301
Identity = 400/479 (83.51%), Postives = 440/479 (91.86%), Query Frame = 0

Query: 3   LTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRME 62
           + M SGG+PSIPSSISPPPV+LPL+NIPGS+GLPL GSIADRLDY+WFQGPDKFFR+R+E
Sbjct: 5   VAMASGGVPSIPSSISPPPVALPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFRSRIE 64

Query: 63  KNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTG 122
           KNRSTVFRTNVPPSFPFI +DP+V+AVLDCKSFAHLFDME+VEK NVLVGDFMPS SFTG
Sbjct: 65  KNRSTVFRTNVPPSFPFILSDPKVIAVLDCKSFAHLFDMEVVEKKNVLVGDFMPSTSFTG 124

Query: 123 NMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGF 182
           NMRVCAYLD SE  HSK+KNF  DILRRSS+IWI ELESNLST WDGIE E+A N++S +
Sbjct: 125 NMRVCAYLDPSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQKSSY 184

Query: 183 RNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFL 242
           RN LQPALFNFFSKTLAGADTAKSPEVAKS YIDVIIWLGLQLVPTIH+GV QPLEEIFL
Sbjct: 185 RNLLQPALFNFFSKTLAGADTAKSPEVAKSAYIDVIIWLGLQLVPTIHLGVFQPLEEIFL 244

Query: 243 HSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFS 302
           HSFRLPFFPIA RY+RLYDF +KEG EVV RGV+EFGL KEEAIHNLIF +GFNAYGGFS
Sbjct: 245 HSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKEEAIHNLIFALGFNAYGGFS 304

Query: 303 LFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPS 362
           LF PVL+DRILND+TGLQQRI++EVR+KS SGLTFESVKEM+L+YS+VYETLRLDPPVP+
Sbjct: 305 LFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSLVYETLRLDPPVPT 364

Query: 363 QYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALL 422
           QYARARKDFKL+S+D+++ IKKGELLCGYQPLVMRDP VF+EPE F PDRFR EKGAALL
Sbjct: 365 QYARARKDFKLSSHDAVFEIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEKGAALL 424

Query: 423 DYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN 481
           DYLFWSNGPQTG  SE NKQCA KDLVVLT VVFVAYIF+RYDSIAGEGGSITA Q+ N
Sbjct: 425 DYLFWSNGPQTGMTSEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITALQKPN 483

BLAST of Chy1G004450 vs. NCBI nr
Match: XP_022969073.1 (fatty acid hydroperoxide lyase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 828 bits (2139), Expect = 1.68e-300
Identity = 399/479 (83.30%), Postives = 438/479 (91.44%), Query Frame = 0

Query: 3   LTMTSGGMPSIPSSISPPPVSLPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRME 62
           + M S G+PSIPSSISPPPV+LPL+NIPGS+GLPL GSIADRLDY+WFQGPDKFFR+RME
Sbjct: 2   VAMASSGVPSIPSSISPPPVTLPLKNIPGSHGLPLFGSIADRLDYFWFQGPDKFFRSRME 61

Query: 63  KNRSTVFRTNVPPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTG 122
           KNRSTVFRTNVPPSFPFI +DP+V AVLDCKSFAHLFDME+VEK NVLVGDFMPS SFTG
Sbjct: 62  KNRSTVFRTNVPPSFPFILSDPKVTAVLDCKSFAHLFDMEVVEKKNVLVGDFMPSTSFTG 121

Query: 123 NMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGF 182
           NMRVCAYLDTSE  HSK+KNF  DILRRSS+IWI ELESNLST WDGIE E+A N++S F
Sbjct: 122 NMRVCAYLDTSESQHSKIKNFTIDILRRSSKIWIPELESNLSTFWDGIESEIANNQKSSF 181

Query: 183 RNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFL 242
           RN LQPALFNFFSKTL GADTAKSPEVAKS +IDVIIWLGLQLVPTIH+G+ QPLEEIFL
Sbjct: 182 RNLLQPALFNFFSKTLVGADTAKSPEVAKSAHIDVIIWLGLQLVPTIHLGIFQPLEEIFL 241

Query: 243 HSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFS 302
           HSFRLPFFPIA RY+RLYDF +KEG EVV RGV+EFGL K+EAIHNLIFT+GFNAYGGFS
Sbjct: 242 HSFRLPFFPIAGRYRRLYDFIEKEGEEVVQRGVSEFGLKKDEAIHNLIFTLGFNAYGGFS 301

Query: 303 LFFPVLLDRILNDKTGLQQRILEEVRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPS 362
           LF PVL+DRILND+TGLQQRI++EVR+KS SGLTFESVKEM+L+YS VYETLRLDPPVP+
Sbjct: 302 LFLPVLMDRILNDRTGLQQRIVDEVRAKSGSGLTFESVKEMELVYSFVYETLRLDPPVPT 361

Query: 363 QYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALL 422
           QYARARKDFKL+S+D++++IKKGELLCGYQPLVMRDP VF+EPE F PDRFR EKGAALL
Sbjct: 362 QYARARKDFKLSSHDAVFDIKKGELLCGYQPLVMRDPNVFEEPEAFKPDRFREEKGAALL 421

Query: 423 DYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSITAFQRAN 481
           DYLFWSNGPQTG  SE NKQCA KDLVVLT VVFVAYIF+RYDSIAGEGGSIT  QR N
Sbjct: 422 DYLFWSNGPQTGMASEHNKQCAAKDLVVLTAVVFVAYIFRRYDSIAGEGGSITTLQRPN 480

BLAST of Chy1G004450 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 438.3 bits (1126), Expect = 7.8e-123
Identity = 207/383 (54.05%), Postives = 280/383 (73.11%), Query Frame = 0

Query: 101 MEIVEKNNVLVGDFMPSISFTGNMRVCAYLDTSEPNHSKVKNFITDILRRSSRIWISELE 160
           M++V+K +VL+GDF PS+ F G +RV  YLDT+EP H+K+K F  + L+RSS++W+ EL 
Sbjct: 1   MDLVDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELR 60

Query: 161 SNLSTMWDGIELEMAKNKQSGFRNFLQPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIW 220
           SNL+  W  IE E++KN  + +   LQ  +F+F   +LAG D + SP++A++G+  +  W
Sbjct: 61  SNLNIFWGTIESEISKNGAASYIFPLQRCIFSFLCASLAGVDASVSPDIAENGWKTINTW 120

Query: 221 LGLQLVPTIHIGVL-QPLEEIFLHSFRLPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFG 280
           L LQ++PT  +GV+ QPLEEI LH++  P   IA  Y++LY+F  +   + +  G  EFG
Sbjct: 121 LALQVIPTAKLGVVPQPLEEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEEFG 180

Query: 281 LTKEEAIHNLIFTMGFNAYGGFSLFFPVLLDRILNDKTGLQQRILEEVRSKSSSG--LTF 340
           LT++EAI NL+F +GFNAYGGFS+F P L+ RI  D +GLQ+RI  EVR    SG  L F
Sbjct: 181 LTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDLNF 240

Query: 341 ESVKEMDLIYSVVYETLRLDPPVPSQYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMR 400
           ++V EM+L+ SVVYETLR  PPVP Q+ARARKDF+++S+D+++ +KKGELLCGYQPLVMR
Sbjct: 241 KTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLVMR 300

Query: 401 DPKVFDEPETFNPDRFRGEKGAALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFV 460
           D  VFDEPE F PDR+ GE G+ LL+YL+WSNGPQTGTPS  NKQCA KD+V LT  + V
Sbjct: 301 DANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASLLV 360

Query: 461 AYIFKRYDSIAGEGGSITAFQRA 481
           A +F RYD+I G+ GSI A  +A
Sbjct: 361 ADLFLRYDTITGDSGSIKAVVKA 383

BLAST of Chy1G004450 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 389.0 bits (998), Expect = 5.4e-108
Identity = 197/453 (43.49%), Postives = 291/453 (64.24%), Query Frame = 0

Query: 24  LPLRNIPGSYGLPLLGSIADRLDYYWFQGPDKFFRTRMEKNRSTVFRTNVPPSFPFISAD 83
           LP+RNIPG+YGLP++G I DR DY++ QG ++FF++R+ K  STV+R N+PP   FI+ +
Sbjct: 52  LPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMPPG-AFIAEN 111

Query: 84  PRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNMRVCAYLDTSEPNHSKVKNF 143
           P+VVA+LD KSF  LFD++ VEK ++  G +MPS   TG  R+ +YLD SEP H K+KN 
Sbjct: 112 PQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEPKHEKLKNL 171

Query: 144 ITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRNFLQPALFNFFSKTLAGADT 203
           +  +L+ S      E ++  S ++D +E E++   ++ F        FNF ++   G + 
Sbjct: 172 LFFLLKSSRNRIFPEFQATYSELFDSLEKELSLKGKADFGGSSDGTAFNFLARAFYGTNP 231

Query: 204 AKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHSFRLPFFPIASRYQRLYDFF 263
           A +   A +  + +  W+   L P + IG+ + +EE  +H+F LP   + S YQRLY+FF
Sbjct: 232 ADTKLKADAPGL-ITKWVLFNLHPLLSIGLPRVIEEPLIHTFSLPPALVKSDYQRLYEFF 291

Query: 264 QKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLFFPVLLDRILNDKTGLQQRI 323
            +   E++     + G+++EEA HNL+F   FN +GG  + FP ++ RI      +  R+
Sbjct: 292 LESAGEILVE-ADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRIGRAGHQVHNRL 351

Query: 324 LEEVRS--KSSSG-LTFESVKEMDLIYSVVYETLRLDPPVPSQYARARKDFKLTSYDSMY 383
            EE+RS  KS+ G LT  ++++M+L  SVVYE LR +PPV +QY RA+KD  + S+D+ +
Sbjct: 352 AEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKKDLVIESHDAAF 411

Query: 384 NIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALLDYLFWSNGPQTGTPSEKN 443
            +K GE+L GYQPL  RDPK+FD  + F P+RF GE+G  LL ++ WSNGP+T TP+  N
Sbjct: 412 KVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSNGPETETPTVGN 471

Query: 444 KQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGS 474
           KQCAGKD VVL   +FV  IF+RYDS   E G+
Sbjct: 472 KQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGT 501

BLAST of Chy1G004450 vs. TAIR 10
Match: AT1G55940.1 (cytochrome P450, family 708, subfamily A, polypeptide 1 )

HSP 1 Score: 77.8 bits (190), Expect = 2.7e-14
Identity = 102/466 (21.89%), Postives = 184/466 (39.48%), Query Frame = 0

Query: 30  PGSYGLPLLGSIADRLDYYWFQGPDKFFRTRMEKNRSTVFRTNVPPSFPFISADPRV--- 89
           PGS G P++G   +    Y F     F + RM K+  ++FRTN+  S   +S DP V   
Sbjct: 182 PGSMGFPVIGETVEFFKPYSFNEIHPFVKKRMFKHGGSLFRTNILGSKTIVSTDPEVNFE 241

Query: 90  VAVLDCKSFAHLFDMEIVE---KNNVLVGDFMPSISFTGNMRVCA--------------- 149
           +   + + F   +   +V    K+N+    F     F   MR  A               
Sbjct: 242 ILKQENRCFIMSYPEALVRIFGKDNLF---FKQGKDFHRYMRHIALQLLGPECLKQRFIQ 301

Query: 150 YLDTSEPNHSKVKNF--ITDILRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRNFL 209
            +D +   H K  +F  + D+   S R+    LE  +  +   I+ E        FR+F 
Sbjct: 302 QIDIATSEHLKSVSFQGVVDVKDTSGRL---ILEQMILMIISNIKPETKSKLIESFRDFS 361

Query: 210 QPALFNFFSKTLAGADTAKSPEVAKSGYIDVIIWLGLQLVPTIHIGVLQPLEEIFLHSFR 269
              + + F  +   A          +G +D  +WL   L P  ++     + ++    F+
Sbjct: 362 FDLVRSPFDPSFWNA--------LYNGLMD-FVWLLNILDPLFYVKARSNVMKMLKRMFK 421

Query: 270 LPFFPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLFFP 329
                  S   +  DF +    EV   G T      EE    LI ++   +Y   S    
Sbjct: 422 ERREEATSDDSKYGDFMETMIYEVEKEGDT----INEERSVELILSLLIASYETTSTMTA 481

Query: 330 VLLDRILNDKTGL-----QQRILEEVRSKSSSGLTFESVKE-MDLIYSVVYETLRLDPPV 389
           + +  I  +   L     +   + + R+   SG+T++  +  M+  + V+ E+LRL    
Sbjct: 482 LTVKFIAENPKVLMELKREHETILQNRADKESGVTWKEYRSMMNFTHMVINESLRLGSLS 541

Query: 390 PSQYARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRG-EKGA 449
           P+ + +A  D ++      Y I  G ++     L+  DP+++++P  FNP R+ G E  +
Sbjct: 542 PAMFRKAVNDVEIKG----YTIPAGWIVLVVPSLLHYDPQIYEQPCEFNPWRWEGKELLS 601

Query: 450 ALLDYLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYD 466
               ++ +  G          + CAG +   L   +F+ ++   YD
Sbjct: 602 GSKTFMAFGGGA---------RLCAGAEFARLQMAIFLHHLVTTYD 615

BLAST of Chy1G004450 vs. TAIR 10
Match: AT1G12740.1 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 74.7 bits (182), Expect = 2.3e-13
Identity = 111/472 (23.52%), Postives = 185/472 (39.19%), Query Frame = 0

Query: 30  PGSYGLPLLGS----------------IADRLDYYWFQGPDKFFRTRMEKNRSTVFRTNV 89
           PGS G PLLG                 I +R+  Y   GP   F+T +   R  +  T+ 
Sbjct: 33  PGSMGFPLLGESIQFFKPNKTSDIPPFIKERVKKY---GP--IFKTNL-VGRPVIVSTDA 92

Query: 90  PPSFPFISADPRVVAVLDCKSFAHLFDMEIVEKNNVLVGDFMPSISFTGNMRVCAYLDTS 149
             S+   + + R        +F H+F     +KN   +  FM    +  NM +  +    
Sbjct: 93  DLSYFVFNQEGRCFQSWYPDTFTHIFG----KKNVGSLHGFM--YKYLKNMVLTLF---- 152

Query: 150 EPNHSKVKNFITDI-LRRSSRIWISELESNLSTMWDGIELEMAKNKQSGFRNFLQPALFN 209
              H  +K  +  + +  + R+   EL SN     D +EL+ A              +F+
Sbjct: 153 --GHDGLKKMLPQVEMTANKRL---ELWSN----QDSVELKDATASM----------IFD 212

Query: 210 FFSKTLAGADTAKSPEVAKSGYIDVI---IWLGLQLVPTIHIGVLQPLEEIFLHSFRLPF 269
             +K L   D  KS E  ++ ++  I   I     +  T +   LQ   +       +  
Sbjct: 213 LTAKKLISHDPDKSSENLRANFVAFIQGLISFPFDIPGTAYHKCLQGRAKAMKMLRNMLQ 272

Query: 270 FPIASRYQRLYDFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLFFPVLL 329
               +  +   DFF     E+   G     LT+E A+ +L+F + F ++   SL   + +
Sbjct: 273 ERRENPRKNPSDFFDYVIEEIQKEGTI---LTEEIAL-DLMFVLLFASFETTSLALTLAI 332

Query: 330 DRILNDKTGLQQRILEE------VRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPSQ 389
            + L+D   + +R+ EE       R  + SGLT+E  K M   +  + ET RL   VP+ 
Sbjct: 333 -KFLSDDPEVLKRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAI 392

Query: 390 YARARKDFKLTSYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALLD 449
           + +A +D K       Y I  G  +    P V  +P+++ +P  FNP R+ G K      
Sbjct: 393 FRKALRDIKFKD----YTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASK 452

Query: 450 YLFWSNGPQTGTPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSIT 476
           +     G          + C G D   L    F+  +  +Y     +GG+IT
Sbjct: 453 HFMAFGGGM--------RFCVGTDFTKLQMAAFLHSLVTKYRWEEIKGGNIT 452

BLAST of Chy1G004450 vs. TAIR 10
Match: AT1G12740.2 (cytochrome P450, family 87, subfamily A, polypeptide 2 )

HSP 1 Score: 71.2 bits (173), Expect = 2.5e-12
Identity = 60/221 (27.15%), Postives = 97/221 (43.89%), Query Frame = 0

Query: 261 DFFQKEGAEVVGRGVTEFGLTKEEAIHNLIFTMGFNAYGGFSLFFPVLLDRILNDKTGLQ 320
           DFF     E+   G     LT+E A+ +L+F + F ++   SL   + + + L+D   + 
Sbjct: 255 DFFDYVIEEIQKEGTI---LTEEIAL-DLMFVLLFASFETTSLALTLAI-KFLSDDPEVL 314

Query: 321 QRILEE------VRSKSSSGLTFESVKEMDLIYSVVYETLRLDPPVPSQYARARKDFKLT 380
           +R+ EE       R  + SGLT+E  K M   +  + ET RL   VP+ + +A +D K  
Sbjct: 315 KRLTEEHETILRNREDADSGLTWEEYKSMTYTFQFINETARLANIVPAIFRKALRDIKFK 374

Query: 381 SYDSMYNIKKGELLCGYQPLVMRDPKVFDEPETFNPDRFRGEKGAALLDYLFWSNGPQTG 440
                Y I  G  +    P V  +P+++ +P  FNP R+ G K      +     G    
Sbjct: 375 D----YTIPAGWAVMVCPPAVHLNPEMYKDPLVFNPSRWEGSKVTNASKHFMAFGGGM-- 434

Query: 441 TPSEKNKQCAGKDLVVLTGVVFVAYIFKRYDSIAGEGGSIT 476
                 + C G D   L    F+  +  +Y     +GG+IT
Sbjct: 435 ------RFCVGTDFTKLQMAAFLHSLVTKYRWEEIKGGNIT 458

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
K4CF701.5e-17161.64Fatty acid hydroperoxide lyase, chloroplastic OS=Solanum lycopersicum OX=4081 GN... [more]
Q9ZSY93.6e-15756.34Linolenate hydroperoxide lyase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
B3LF831.1e-12154.05Probable inactive linolenate hydroperoxide lyase OS=Arabidopsis thaliana OX=3702... [more]
P484171.3e-10942.77Allene oxide synthase, chloroplastic OS=Linum usitatissimum OX=4006 GN=CYP74A PE... [more]
Q407786.2e-10943.76Allene oxide synthase OS=Parthenium argentatum OX=35935 GN=CYP74A2 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0K5I01.3e-27498.3213-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G041960 PE=4 SV=1[more]
A0A1S4E1964.7e-26995.64fatty acid hydroperoxide lyase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Q66UT11.8e-25288.5713-hydroperoxide lyase OS=Citrullus lanatus OX=3654 PE=2 SV=1[more]
A0A6J1HZX61.1e-23883.30fatty acid hydroperoxide lyase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1GJZ63.3e-23882.88fatty acid hydroperoxide lyase, chloroplastic OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
XP_004144503.10.098.34fatty acid hydroperoxide lyase, chloroplastic [Cucumis sativus] >KAE8645736.1 hy... [more]
XP_016901760.10.095.64PREDICTED: fatty acid hydroperoxide lyase, chloroplastic [Cucumis melo][more]
AAU12570.10.088.5713-hydroperoxide lyase [Citrullus lanatus][more]
XP_023554457.12.29e-30183.51fatty acid hydroperoxide lyase, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022969073.11.68e-30083.30fatty acid hydroperoxide lyase, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT4G15440.17.8e-12354.05hydroperoxide lyase 1 [more]
AT5G42650.15.4e-10843.49allene oxide synthase [more]
AT1G55940.12.7e-1421.89cytochrome P450, family 708, subfamily A, polypeptide 1 [more]
AT1G12740.12.3e-1323.52cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
AT1G12740.22.5e-1227.15cytochrome P450, family 87, subfamily A, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 380..394
score: 26.15
coord: 396..414
score: 36.84
coord: 343..359
score: 39.82
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 24..476
e-value: 4.8E-96
score: 324.5
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 24..467
IPR001128Cytochrome P450PFAMPF00067p450coord: 319..419
e-value: 5.3E-17
score: 61.7
NoneNo IPR availablePANTHERPTHR24286:SF49INACTIVE LINOLENATE HYDROPEROXIDE LYASE-RELATEDcoord: 19..471
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 19..471

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G004450.1Chy1G004450.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen