Chy1G004380 (gene) Cucumber (hystrix) v1

Overview
NameChy1G004380
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionMitogen-activated protein kinase kinase kinase 2
LocationchrH01: 3150118 .. 3151236 (-)
RNA-Seq ExpressionChy1G004380
SyntenyChy1G004380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTGGACCCGACGATCCCCCGTCGGCAATGGCTCCTCCGCCACCGTCTACCTCGCCTCCACTGCCTCCTCCGGCCAACTATTTGCTGTCAAGTCCGCCGAGCTTCTCAAATCAGACTTCTTGAAAATAGAGCAAAGGGTTCTTTCTTCCCTGTCCAACCCTTCAATTGTCGGCTACAGGGGATTCGATGTAACCACAGAAAATGGGAAGCTAATGTACAATCTCTTCATGGAGTACGCTGCCGGTGGTACTCTCACTGATGAGATATTCCGGCGTGGTGGCCGGATTAAGGAGCCAACGGCGGCGTTTTACACGCGTGAAATTGTTAGAGGATTGGAGTACTTGCACAAACAGGGGTTGGTGCATTGTGATATCAAAGCTAAGAACATTTTAATTGCTGGAGATGGTTTGAAGATTGCTGATTTTGGATGCTCCAGATGGGTTTGTGAGGCCGAGGCAGTAATAGGCGGAACGCCGATGTTCATGGCACCGGAGGTGGCGCGTGGGGAAAAACAGGGGATTTCCTCTGACATATGGGCACTTGGGTGTACATTAATCGAAATGGTCACCGGCGCTCCACCATGGAAAATTACCGACGACCCAGTTTCGGTGTTGTACCGGATCGGATATTCTGGCGAGTCTCCGGAAATTCCGAGCTTTCTGTCGGAGAAAGGAAAGGATTTTTTAAGGAAATGCTTGAGAAGGGAAGCAACAGAAAGATGGAGTGCGAGTCAACTTCTTGAACATCCATTTTTGGGGGAATTGAGTTCTGGGTTGGAGGAAATCAAGGAATTACATTTATATTCTCAATCTCCGACGAGTATTTTAGATCAAAGCCTCTGGAATTCCTTAGAAGAAGAATCAGAAACTTTGTTGAGAACAGAGCAATGGGAGGATGACAGAATCGAACGGCTGGCTACGTTTTCAGGAGAAATTAAGTGGGAATTGGGAGACGAGAATTGGATCACAATCAGAAGCTATGTAGATGGTGAAGATGAAGATGAATTAAGTTTTAGTAGAAATGATTTAGACTTAGTGGAAGAGGGAAGAATAGTTAGTGAGAAAATCCAGTATTTGGAATTGTTAGATAAAACAGTTAGCTTTAGAGTAAGGTAG

mRNA sequence

ATGGCCTGGACCCGACGATCCCCCGTCGGCAATGGCTCCTCCGCCACCGTCTACCTCGCCTCCACTGCCTCCTCCGGCCAACTATTTGCTGTCAAGTCCGCCGAGCTTCTCAAATCAGACTTCTTGAAAATAGAGCAAAGGGTTCTTTCTTCCCTGTCCAACCCTTCAATTGTCGGCTACAGGGGATTCGATGTAACCACAGAAAATGGGAAGCTAATGTACAATCTCTTCATGGAGTACGCTGCCGGTGGTACTCTCACTGATGAGATATTCCGGCGTGGTGGCCGGATTAAGGAGCCAACGGCGGCGTTTTACACGCGTGAAATTGTTAGAGGATTGGAGTACTTGCACAAACAGGGGTTGGTGCATTGTGATATCAAAGCTAAGAACATTTTAATTGCTGGAGATGGTTTGAAGATTGCTGATTTTGGATGCTCCAGATGGGTTTGTGAGGCCGAGGCAGTAATAGGCGGAACGCCGATGTTCATGGCACCGGAGGTGGCGCGTGGGGAAAAACAGGGGATTTCCTCTGACATATGGGCACTTGGGTGTACATTAATCGAAATGGTCACCGGCGCTCCACCATGGAAAATTACCGACGACCCAGTTTCGGTGTTGTACCGGATCGGATATTCTGGCGAGTCTCCGGAAATTCCGAGCTTTCTGTCGGAGAAAGGAAAGGATTTTTTAAGGAAATGCTTGAGAAGGGAAGCAACAGAAAGATGGAGTGCGAGTCAACTTCTTGAACATCCATTTTTGGGGGAATTGAGTTCTGGGTTGGAGGAAATCAAGGAATTACATTTATATTCTCAATCTCCGACGAGTATTTTAGATCAAAGCCTCTGGAATTCCTTAGAAGAAGAATCAGAAACTTTGTTGAGAACAGAGCAATGGGAGGATGACAGAATCGAACGGCTGGCTACGTTTTCAGGAGAAATTAAGTGGGAATTGGGAGACGAGAATTGGATCACAATCAGAAGCTATGTAGATGGTGAAGATGAAGATGAATTAAGTTTTAGTAGAAATGATTTAGACTTAGTGGAAGAGGGAAGAATAGTTAGTGAGAAAATCCAGTATTTGGAATTGTTAGATAAAACAGTTAGCTTTAGAGTAAGGTAG

Coding sequence (CDS)

ATGGCCTGGACCCGACGATCCCCCGTCGGCAATGGCTCCTCCGCCACCGTCTACCTCGCCTCCACTGCCTCCTCCGGCCAACTATTTGCTGTCAAGTCCGCCGAGCTTCTCAAATCAGACTTCTTGAAAATAGAGCAAAGGGTTCTTTCTTCCCTGTCCAACCCTTCAATTGTCGGCTACAGGGGATTCGATGTAACCACAGAAAATGGGAAGCTAATGTACAATCTCTTCATGGAGTACGCTGCCGGTGGTACTCTCACTGATGAGATATTCCGGCGTGGTGGCCGGATTAAGGAGCCAACGGCGGCGTTTTACACGCGTGAAATTGTTAGAGGATTGGAGTACTTGCACAAACAGGGGTTGGTGCATTGTGATATCAAAGCTAAGAACATTTTAATTGCTGGAGATGGTTTGAAGATTGCTGATTTTGGATGCTCCAGATGGGTTTGTGAGGCCGAGGCAGTAATAGGCGGAACGCCGATGTTCATGGCACCGGAGGTGGCGCGTGGGGAAAAACAGGGGATTTCCTCTGACATATGGGCACTTGGGTGTACATTAATCGAAATGGTCACCGGCGCTCCACCATGGAAAATTACCGACGACCCAGTTTCGGTGTTGTACCGGATCGGATATTCTGGCGAGTCTCCGGAAATTCCGAGCTTTCTGTCGGAGAAAGGAAAGGATTTTTTAAGGAAATGCTTGAGAAGGGAAGCAACAGAAAGATGGAGTGCGAGTCAACTTCTTGAACATCCATTTTTGGGGGAATTGAGTTCTGGGTTGGAGGAAATCAAGGAATTACATTTATATTCTCAATCTCCGACGAGTATTTTAGATCAAAGCCTCTGGAATTCCTTAGAAGAAGAATCAGAAACTTTGTTGAGAACAGAGCAATGGGAGGATGACAGAATCGAACGGCTGGCTACGTTTTCAGGAGAAATTAAGTGGGAATTGGGAGACGAGAATTGGATCACAATCAGAAGCTATGTAGATGGTGAAGATGAAGATGAATTAAGTTTTAGTAGAAATGATTTAGACTTAGTGGAAGAGGGAAGAATAGTTAGTGAGAAAATCCAGTATTTGGAATTGTTAGATAAAACAGTTAGCTTTAGAGTAAGGTAG

Protein sequence

MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGYRGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQGLVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIWALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATERWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWEDDRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELSFSRNDLDLVEEGRIVSEKIQYLELLDKTVSFRVR*
Homology
BLAST of Chy1G004380 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 333.6 bits (854), Expect = 2.9e-90
Identity = 183/369 (49.59%), Postives = 242/369 (65.58%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           M WTR   +G GS+ATVY A+  +S ++ AVKS+E+ +S+FL+ E ++LSSLS+P ++GY
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  RGFDVTTE-NGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQ 120
           RG +   E NG +MYNL MEYA  GTLTD   + GGR+ E     YTR+I++GLEY+H +
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GLVHCDIKAKNILIAGDG-LKIADFGCSRWVCEA-EAVIGGTPMFMAPEVARGEKQGISS 180
           G+VHCD+K  N++I+  G  KIADFGC++ V    E+ + GTP FMAPEVARGEKQG  S
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DIWALGCTLIEMVTGAPPWKITD---DPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCL 240
           DIWA+GCT+IEMVTG+PPW   D   DPVSVLYR+GYS E+PE+P  L+E+ KDFL KCL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 RREATERWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESE---- 300
           +REA ERW+A+QLL HPFL      +E +    L S SPTS+ DQ+ W S+EEE E    
Sbjct: 241 KREANERWTATQLLNHPFL-TTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETE 300

Query: 301 -------TLLRTEQW--EDDRIERLATFSG--EIKWELGDENWITIRSYVDGE----DED 345
                   L R   W    +RI RL    G    + ++   +WI +R+  +G      + 
Sbjct: 301 EIQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGTRCDMEGGDWIMVRARCEGTMISGSQK 360

BLAST of Chy1G004380 vs. ExPASy Swiss-Prot
Match: Q9ZVP5 (Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK18 PE=1 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 6.8e-87
Identity = 180/347 (51.87%), Postives = 229/347 (65.99%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           M WTR   +G GS+ATV  A+   SG+  AVKSAE  +S+FL+ E ++LSSL++P ++GY
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  RGFDVTTE----NGK-LMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEY 120
           RG ++T E    NG+   Y+L MEYA  GTLTD   + GG I E     YTR+I+ GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 LH-KQGLVHCDIKAKNILIAGDG-LKIADFGCSRWV-CEAEAVIGGTPMFMAPEVARGEK 180
           +H  +G+ HCDIK  N+L+  +G  KIADFGC++WV  E    + GTP FMAPE ARGE+
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGISSDIWALGCTLIEMVTGAPPWKITD--DPVSVLYRIGYSGESPEIPSFLSEKGKDFL 240
           QG  SDIWA+GCT+IEMVTG+ PW   D  DPVSVLYR+GY GE PE+P  L+E+ KDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 RKCLRREATERWSASQLLEHPFL----GELSSGLEEIKELHLYSQSPTSILDQSLWNSLE 300
            KCL++EATERW+ASQLL HPFL     EL +G        L + SPTS+ DQ  W S+E
Sbjct: 241 GKCLKKEATERWTASQLLNHPFLVNKEPELVTG--------LVTNSPTSVTDQMFWRSVE 300

Query: 301 EE-SETLLRTEQWEDDRIERLATFSG------EIKWELGDENWITIR 327
           EE SE   R+  WE    ER+   S       E  W+L  E+WIT+R
Sbjct: 301 EEVSED--RSSWWECHEDERIGVLSWIGHVVVESTWDLDGEDWITVR 337

BLAST of Chy1G004380 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.7e-50
Identity = 127/349 (36.39%), Postives = 189/349 (54.15%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLAS-TASSGQ---LFAVKSAELLKSDFLKIEQRVLSSLSN-P 60
           M W R   +G G+ +TV  A+ + +SG    L AVKS +   +  L  E+ VL SL + P
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  SIVGYRGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGR-IKEPTAAFYTREIVRGLE 120
            I+   G D T ENG+ M+NL +EYA+ G+L   + + GG  + E T   +T  ++RGL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 YLHKQGLVHCDIKAKNILIAGDG-LKIADFGCSRWV------CEAEAVIGGTPMFMAPEV 180
           ++H +G  HCDIK  NIL+  DG +KIADFG +  V            I GTP++MAPE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 ARGEKQGISSDIWALGCTLIEMVTGAPPWKITDDP--VSVLYRIGYSGESPEIPSFLSEK 240
               + G ++D+WALGC ++EM +G   W + +    +S+L RIG   E P+IP  LSE+
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 GKDFLRKCLRREATERWSASQLLEHPFL------GELSSGLEEIKELHLYSQSPTSILDQ 300
           GKDFL KC  ++  +RW+A  LL H F+          + + ++K+      SP    + 
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEF 300

Query: 301 SLWNSLEEESETLLRTEQWEDDRIERLATF-SGEI-KWELGDENWITIR 327
             W+S   +S          D  +ERL +  SG I  W +G  +W+T+R
Sbjct: 301 DDWDSFTLDSNPSF------DSPVERLGSLVSGSIPDWSVGG-SWLTVR 342

BLAST of Chy1G004380 vs. ExPASy Swiss-Prot
Match: Q40541 (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097 GN=NPK1 PE=1 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 8.9e-47
Identity = 107/268 (39.93%), Postives = 153/268 (57.09%), Query Frame = 0

Query: 3   WTRRSPVGNGSSATVYLASTASSGQLFAVK---------SAELLKSDFLKIEQRV--LSS 62
           W +   +G G+   VY+     SG+L A+K         S E  ++   ++E+ V  L +
Sbjct: 80  WRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEVNLLKN 139

Query: 63  LSNPSIVGYRGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVR 122
           LS+P+IV Y G     E G L  N+ +E+  GG+++  +  + G   E     YT++++ 
Sbjct: 140 LSHPNIVRYLG--TAREAGSL--NILLEFVPGGSIS-SLLGKFGSFPESVIRMYTKQLLL 199

Query: 123 GLEYLHKQGLVHCDIKAKNILIAGDG-LKIADFGCSRWVCEAEAVIG-----GTPMFMAP 182
           GLEYLHK G++H DIK  NIL+   G +K+ADFG S+ V E   + G     GTP +MAP
Sbjct: 200 GLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWMAP 259

Query: 183 EVARGEKQGISSDIWALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEK 242
           EV        S+DIW++GCT+IEM TG PPW      V+ L+ IG +   P IP  LS +
Sbjct: 260 EVILQTGHSFSADIWSVGCTIIEMATGKPPWSQQYQEVAALFHIGTTKSHPPIPEHLSAE 319

Query: 243 GKDFLRKCLRREATERWSASQLLEHPFL 254
            KDFL KCL++E   R SAS LL+HPF+
Sbjct: 320 SKDFLLKCLQKEPHLRHSASNLLQHPFV 342

BLAST of Chy1G004380 vs. ExPASy Swiss-Prot
Match: O22042 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=ANP3 PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.7e-45
Identity = 103/268 (38.43%), Postives = 150/268 (55.97%), Query Frame = 0

Query: 3   WTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDF-----------LKIEQRVLSS 62
           W +   +G G+   VY+     SG+L A+K   +  S             L+ E ++L +
Sbjct: 68  WRKGELIGCGAFGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLKN 127

Query: 63  LSNPSIVGYRGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVR 122
           LS+P+IV Y G     E+  L  N+ ME+  GG+++  +  + G   EP    YT++++ 
Sbjct: 128 LSHPNIVRYLG--TVRESDSL--NILMEFVPGGSIS-SLLEKFGSFPEPVIIMYTKQLLL 187

Query: 123 GLEYLHKQGLVHCDIKAKNILIAGDG-LKIADFGCSRWVCEAEAVIG-----GTPMFMAP 182
           GLEYLH  G++H DIK  NIL+   G +++ADFG S+ V E   V G     GTP +MAP
Sbjct: 188 GLEYLHNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYWMAP 247

Query: 183 EVARGEKQGISSDIWALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEK 242
           EV        S+DIW++GCT+IEM TG PPW       + +  IG +   P IP  LS +
Sbjct: 248 EVILQTGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIPEDLSPE 307

Query: 243 GKDFLRKCLRREATERWSASQLLEHPFL 254
            KDFL KCL +E + R SA++LL+HPF+
Sbjct: 308 AKDFLMKCLHKEPSLRLSATELLQHPFV 330

BLAST of Chy1G004380 vs. ExPASy TrEMBL
Match: A0A0A0K6U1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G043040 PE=3 SV=1)

HSP 1 Score: 728.8 bits (1880), Expect = 1.2e-206
Identity = 362/372 (97.31%), Postives = 369/372 (99.19%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIE+RVLSSLSNPSIVGY
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIERRVLSSLSNPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           +GFDVT ENGKLMYNLFMEYAAGGTL DEIFRRGGRIKE TAAFYTREIVRGLEYLHKQG
Sbjct: 61  KGFDVTRENGKLMYNLFMEYAAGGTLADEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCDIKAKNILIAGDGLKIADFGCSRWVCE+EAVIGGTPMFMAPEVARGEKQGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCESEAVIGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE
Sbjct: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWED 300
           RWSASQLLEHPFLGELSSGLEEIKELHLYS+SPTSILDQSLWNSLEEESETLLRTEQW+D
Sbjct: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSESPTSILDQSLWNSLEEESETLLRTEQWDD 300

Query: 301 DRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELSFSRNDLDLVEEGRIVSEKIQYL 360
           DRIERLATFSGEIKWELGDENWITIRSYVDGEDEDEL+FSRNDLDLVEEGRIVSE+IQYL
Sbjct: 301 DRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELNFSRNDLDLVEEGRIVSEEIQYL 360

Query: 361 ELLDKTVSFRVR 373
           ELLDKTVSFRVR
Sbjct: 361 ELLDKTVSFRVR 372

BLAST of Chy1G004380 vs. ExPASy TrEMBL
Match: A0A5A7T9T3 (Mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G004350 PE=3 SV=1)

HSP 1 Score: 701.4 bits (1809), Expect = 2.0e-198
Identity = 353/378 (93.39%), Postives = 363/378 (96.03%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTRRSPVGNGSSATVYLASTASSG+LFAVKSAELLKSDFLK EQR+LSSLS+PSIVGY
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGELFAVKSAELLKSDFLKTEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           RGFDVT ENGKLMYNLFMEYA GGTLTDEIFRRGGRIKE TAAFYTREIVRGLEYLHKQG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYADGGTLTDEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCDIKAKNILIAGDGLKIADFGCS+WVCEAEAV GGTPMFMAPEVARGEKQGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSKWVCEAEAVKGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           ALGCTLIEMVTGA PWK TDDPVSVLYRIGYSGESPE+PSFLSEKGKDFLRKCLRREATE
Sbjct: 181 ALGCTLIEMVTGALPWKTTDDPVSVLYRIGYSGESPEVPSFLSEKGKDFLRKCLRREATE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQW-- 300
           RWSASQLLEHPFLGELSSGLEEIKELH YS+SPTSILDQSLWNSLEEESETLL+TEQW  
Sbjct: 241 RWSASQLLEHPFLGELSSGLEEIKELHSYSESPTSILDQSLWNSLEEESETLLKTEQWDD 300

Query: 301 ---EDDRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELSFSRNDL-DLVEEGRIVS 360
              +DDRIERLATFSGEIKWELGDENWITIRSYVDGEDEDEL+FSRNDL DL EEGRIVS
Sbjct: 301 EDEDDDRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELNFSRNDLDDLEEEGRIVS 360

Query: 361 EKIQYLELLDKTVSFRVR 373
           EK+QYLELLDKTVSFRVR
Sbjct: 361 EKVQYLELLDKTVSFRVR 378

BLAST of Chy1G004380 vs. ExPASy TrEMBL
Match: A0A1S3C1N1 (mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo OX=3656 GN=LOC103495608 PE=3 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 1.7e-197
Identity = 352/378 (93.12%), Postives = 361/378 (95.50%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTRRSPVGNGSSATVYLASTASSG+LFAVKSAELLKSDFLK EQR+LSSLS+PSIVGY
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGELFAVKSAELLKSDFLKTEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           RGFDVT ENGKLMYNLFMEYA GGTLTDEIFRRGGRIKE TAAFYTREIVRGLEYLHKQG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYADGGTLTDEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCDIKAKNILIAGDGLKIADFGCS+WVCEAEAV GGTPMFMAPEVARGEKQGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSKWVCEAEAVKGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           ALGCTLIEMVTGA PWK TDDPVSVLYRIGYSGESPE+PSFLSEKG DFLRKCLRREATE
Sbjct: 181 ALGCTLIEMVTGALPWKTTDDPVSVLYRIGYSGESPEVPSFLSEKGNDFLRKCLRREATE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQW-- 300
           RWSASQLLEHPFLGELSSGLEEIKELH YS+SPTSILDQSLWNSLEEESETLL+TEQW  
Sbjct: 241 RWSASQLLEHPFLGELSSGLEEIKELHSYSESPTSILDQSLWNSLEEESETLLKTEQWDD 300

Query: 301 ---EDDRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELSFSRNDL-DLVEEGRIVS 360
              +DDRIERLATFSGEIKWE GDENWITIRSYVDGEDEDEL+FSRNDL DL EEGRIVS
Sbjct: 301 EDEDDDRIERLATFSGEIKWEFGDENWITIRSYVDGEDEDELNFSRNDLDDLEEEGRIVS 360

Query: 361 EKIQYLELLDKTVSFRVR 373
           EKIQYLELLDKTVSFRVR
Sbjct: 361 EKIQYLELLDKTVSFRVR 378

BLAST of Chy1G004380 vs. ExPASy TrEMBL
Match: A0A6J1GLZ4 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111455196 PE=3 SV=1)

HSP 1 Score: 533.5 bits (1373), Expect = 7.1e-148
Identity = 260/333 (78.08%), Postives = 293/333 (87.99%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTR + VG+GS ATVYLA+++SSG LFAVKSAELL SDFL+ EQR+LSSLS+PSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           +GFDVTTENGKLMYNLFMEYAAGG+L DEI RRGGRIKE TAAFYTR+IVRGLEYLHK+G
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCD+KA+NILI  DGLKIADFGCS+WV E+ A + GTPMFMAPEVARGE QGISSDIW
Sbjct: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           ALGCTLIEMV+GAPPW    DPVS LYRI YSG+SPEIPSFLSEK KDFL KCLRR  TE
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWED 300
           RWSA++LL+HPFLGELS G E I+ELH   +SPTSILDQ  WNSLEE S+TLL+TE WED
Sbjct: 241 RWSANRLLKHPFLGELSFGFEIIRELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300

Query: 301 DRIERLATFSGEIKWELGDENWITIRSYVDGED 334
           DRI+RL+ FSGEIKWE+ D+NWITIRS V+ ++
Sbjct: 301 DRIKRLSMFSGEIKWEMRDDNWITIRSCVNDDN 333

BLAST of Chy1G004380 vs. ExPASy TrEMBL
Match: A0A6J1HZ76 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111468247 PE=3 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 1.0e-146
Identity = 259/333 (77.78%), Postives = 290/333 (87.09%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTR + VG+GS ATVYLA+++SSG LFAVKSAELL SDFL+ EQR+LSSLS+PSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLQTEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           +GFDVTTENGKLMYNLFMEYAA G+L DEI RRGGRIKE TAAFYTR+IVRGLEYLHK+G
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAACGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCD+KAKNILI GDGLKIADFGCS+WV E+ A + GTPMFMAPEVARGE QGISSDIW
Sbjct: 121 LVHCDVKAKNILIGGDGLKIADFGCSKWVLESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           ALGCTLIEMV+GAPPW    DPVS LYRI YSG+SPEIPSFLS   KDFL KCLRR+  E
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSATAKDFLGKCLRRDPAE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWED 300
           RWS SQLL+HPFLGELS G E IKELH   +SPTSILDQ  WNSLEE S+TLL+TE WED
Sbjct: 241 RWSTSQLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300

Query: 301 DRIERLATFSGEIKWELGDENWITIRSYVDGED 334
           DRI+RL+ FSGEIKW++ D+NWITIRS V+ ++
Sbjct: 301 DRIKRLSVFSGEIKWKMRDDNWITIRSCVNDDN 333

BLAST of Chy1G004380 vs. NCBI nr
Match: XP_004144594.1 (mitogen-activated protein kinase kinase kinase 18 [Cucumis sativus] >KGN43511.1 hypothetical protein Csa_020329 [Cucumis sativus])

HSP 1 Score: 722 bits (1864), Expect = 2.93e-262
Identity = 362/372 (97.31%), Postives = 369/372 (99.19%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIE+RVLSSLSNPSIVGY
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIERRVLSSLSNPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           +GFDVT ENGKLMYNLFMEYAAGGTL DEIFRRGGRIKE TAAFYTREIVRGLEYLHKQG
Sbjct: 61  KGFDVTRENGKLMYNLFMEYAAGGTLADEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCDIKAKNILIAGDGLKIADFGCSRWVCE+EAVIGGTPMFMAPEVARGEKQGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCESEAVIGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE
Sbjct: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWED 300
           RWSASQLLEHPFLGELSSGLEEIKELHLYS+SPTSILDQSLWNSLEEESETLLRTEQW+D
Sbjct: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSESPTSILDQSLWNSLEEESETLLRTEQWDD 300

Query: 301 DRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELSFSRNDLDLVEEGRIVSEKIQYL 360
           DRIERLATFSGEIKWELGDENWITIRSYVDGEDEDEL+FSRNDLDLVEEGRIVSE+IQYL
Sbjct: 301 DRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELNFSRNDLDLVEEGRIVSEEIQYL 360

Query: 361 ELLDKTVSFRVR 372
           ELLDKTVSFRVR
Sbjct: 361 ELLDKTVSFRVR 372

BLAST of Chy1G004380 vs. NCBI nr
Match: KAA0040234.1 (mitogen-activated protein kinase kinase kinase 2 [Cucumis melo var. makuwa] >TYK07114.1 mitogen-activated protein kinase kinase kinase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 695 bits (1793), Expect = 2.43e-251
Identity = 353/378 (93.39%), Postives = 363/378 (96.03%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTRRSPVGNGSSATVYLASTASSG+LFAVKSAELLKSDFLK EQR+LSSLS+PSIVGY
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGELFAVKSAELLKSDFLKTEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           RGFDVT ENGKLMYNLFMEYA GGTLTDEIFRRGGRIKE TAAFYTREIVRGLEYLHKQG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYADGGTLTDEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCDIKAKNILIAGDGLKIADFGCS+WVCEAEAV GGTPMFMAPEVARGEKQGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSKWVCEAEAVKGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           ALGCTLIEMVTGA PWK TDDPVSVLYRIGYSGESPE+PSFLSEKGKDFLRKCLRREATE
Sbjct: 181 ALGCTLIEMVTGALPWKTTDDPVSVLYRIGYSGESPEVPSFLSEKGKDFLRKCLRREATE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWED 300
           RWSASQLLEHPFLGELSSGLEEIKELH YS+SPTSILDQSLWNSLEEESETLL+TEQW+D
Sbjct: 241 RWSASQLLEHPFLGELSSGLEEIKELHSYSESPTSILDQSLWNSLEEESETLLKTEQWDD 300

Query: 301 -----DRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELSFSRNDLD-LVEEGRIVS 360
                DRIERLATFSGEIKWELGDENWITIRSYVDGEDEDEL+FSRNDLD L EEGRIVS
Sbjct: 301 EDEDDDRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELNFSRNDLDDLEEEGRIVS 360

Query: 361 EKIQYLELLDKTVSFRVR 372
           EK+QYLELLDKTVSFRVR
Sbjct: 361 EKVQYLELLDKTVSFRVR 378

BLAST of Chy1G004380 vs. NCBI nr
Match: XP_008455448.1 (PREDICTED: mitogen-activated protein kinase kinase kinase 2 [Cucumis melo])

HSP 1 Score: 692 bits (1785), Expect = 4.02e-250
Identity = 352/378 (93.12%), Postives = 361/378 (95.50%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTRRSPVGNGSSATVYLASTASSG+LFAVKSAELLKSDFLK EQR+LSSLS+PSIVGY
Sbjct: 1   MAWTRRSPVGNGSSATVYLASTASSGELFAVKSAELLKSDFLKTEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           RGFDVT ENGKLMYNLFMEYA GGTLTDEIFRRGGRIKE TAAFYTREIVRGLEYLHKQG
Sbjct: 61  RGFDVTRENGKLMYNLFMEYADGGTLTDEIFRRGGRIKEATAAFYTREIVRGLEYLHKQG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCDIKAKNILIAGDGLKIADFGCS+WVCEAEAV GGTPMFMAPEVARGEKQGISSDIW
Sbjct: 121 LVHCDIKAKNILIAGDGLKIADFGCSKWVCEAEAVKGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           ALGCTLIEMVTGA PWK TDDPVSVLYRIGYSGESPE+PSFLSEKG DFLRKCLRREATE
Sbjct: 181 ALGCTLIEMVTGALPWKTTDDPVSVLYRIGYSGESPEVPSFLSEKGNDFLRKCLRREATE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWED 300
           RWSASQLLEHPFLGELSSGLEEIKELH YS+SPTSILDQSLWNSLEEESETLL+TEQW+D
Sbjct: 241 RWSASQLLEHPFLGELSSGLEEIKELHSYSESPTSILDQSLWNSLEEESETLLKTEQWDD 300

Query: 301 -----DRIERLATFSGEIKWELGDENWITIRSYVDGEDEDELSFSRNDLD-LVEEGRIVS 360
                DRIERLATFSGEIKWE GDENWITIRSYVDGEDEDEL+FSRNDLD L EEGRIVS
Sbjct: 301 EDEDDDRIERLATFSGEIKWEFGDENWITIRSYVDGEDEDELNFSRNDLDDLEEEGRIVS 360

Query: 361 EKIQYLELLDKTVSFRVR 372
           EKIQYLELLDKTVSFRVR
Sbjct: 361 EKIQYLELLDKTVSFRVR 378

BLAST of Chy1G004380 vs. NCBI nr
Match: XP_038887174.1 (mitogen-activated protein kinase kinase kinase 18 [Benincasa hispida])

HSP 1 Score: 605 bits (1559), Expect = 2.69e-215
Identity = 309/386 (80.05%), Postives = 338/386 (87.56%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTR+S +G+GSSATVYLA+  SSG+LFAVKSA+LLKSDFLK EQR+LSSLS+PSIVGY
Sbjct: 1   MAWTRQSAIGHGSSATVYLATAVSSGELFAVKSADLLKSDFLKTEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           RGFDVT ENGKLM++LFMEYAA GTLTDEI RRGGRIKE  AAFYTR+IV GLEYLHKQG
Sbjct: 61  RGFDVTRENGKLMFHLFMEYAASGTLTDEIVRRGGRIKETAAAFYTRQIVGGLEYLHKQG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
            VHCDIKAKNILI  DGLKIADFGCS+WVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW
Sbjct: 121 FVHCDIKAKNILIGEDGLKIADFGCSKWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           +LGCTLIEMVTGAPPW I  DPVSVLYRIGYSGESPEIPSFLSEK KDFL KCLRR+ATE
Sbjct: 181 SLGCTLIEMVTGAPPWPIPGDPVSVLYRIGYSGESPEIPSFLSEKAKDFLSKCLRRDATE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWED 300
           RWSA+QLLEHPFL ELS G EEIKELH +S+SPTSILDQSLWNSL+ ESETLL+TEQW+D
Sbjct: 241 RWSANQLLEHPFLRELSHGFEEIKELHPHSKSPTSILDQSLWNSLKTESETLLKTEQWDD 300

Query: 301 D-----RIERLATFSGEIKWELGDENWITIRSYVDGEDEDE----------LSFSRNDLD 360
           D     RI+RLA+ SGE+  E GDENWITIR+ VD EDEDE          ++F RNDL+
Sbjct: 301 DDDHDDRIQRLASISGEMNLEFGDENWITIRNCVDSEDEDEDESMFRSTSEMNFRRNDLN 360

Query: 361 LVEEGRIVSEKIQYLELLDKTVSFRV 371
           +VE+GRI S KIQ LELLDKTVSFRV
Sbjct: 361 IVEQGRIGSGKIQCLELLDKTVSFRV 386

BLAST of Chy1G004380 vs. NCBI nr
Match: KAG6572287.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 530 bits (1364), Expect = 1.00e-186
Identity = 261/329 (79.33%), Postives = 290/329 (88.15%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           MAWTR + VG+GS ATVYLA+++SSG LFAVKSAELL SDFL+ EQR+LSSLS+PSIVGY
Sbjct: 1   MAWTRLAAVGHGSFATVYLAASSSSGDLFAVKSAELLNSDFLETEQRILSSLSHPSIVGY 60

Query: 61  RGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQG 120
           +GFDVTTENGKLMYNLFMEYAAGG+L DEI RRGGRIKE TAAFYTR+IVRGLEYLHK+G
Sbjct: 61  KGFDVTTENGKLMYNLFMEYAAGGSLADEIVRRGGRIKEVTAAFYTRQIVRGLEYLHKRG 120

Query: 121 LVHCDIKAKNILIAGDGLKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIW 180
           LVHCD+KA+NILI  DGLKIADFGCS+WV E+ A + GTPMFMAPEVARGE QGISSDIW
Sbjct: 121 LVHCDVKARNILIGADGLKIADFGCSKWVRESPAAVCGTPMFMAPEVARGETQGISSDIW 180

Query: 181 ALGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATE 240
           ALGCTLIEMV+GAPPW    DPVS LYRI YSG+SPEIPSFLSEK KDFL KCLRR  TE
Sbjct: 181 ALGCTLIEMVSGAPPWPTAGDPVSTLYRIAYSGDSPEIPSFLSEKAKDFLGKCLRRNPTE 240

Query: 241 RWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWED 300
           RWSA++LL+HPFLGELS G E IKELH   +SPTSILDQ  WNSLEE S+TLL+TE WED
Sbjct: 241 RWSANRLLKHPFLGELSFGFEIIKELHSDFESPTSILDQGPWNSLEEGSQTLLKTETWED 300

Query: 301 DRIERLATFSGEIKWELGDENWITIRSYV 329
           DRI+RL+ FSGEIKWE+ D+NWITIRS V
Sbjct: 301 DRIKRLSMFSGEIKWEMRDDNWITIRSCV 329

BLAST of Chy1G004380 vs. TAIR 10
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17 )

HSP 1 Score: 333.6 bits (854), Expect = 2.1e-91
Identity = 183/369 (49.59%), Postives = 242/369 (65.58%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           M WTR   +G GS+ATVY A+  +S ++ AVKS+E+ +S+FL+ E ++LSSLS+P ++GY
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  RGFDVTTE-NGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQ 120
           RG +   E NG +MYNL MEYA  GTLTD   + GGR+ E     YTR+I++GLEY+H +
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GLVHCDIKAKNILIAGDG-LKIADFGCSRWVCEA-EAVIGGTPMFMAPEVARGEKQGISS 180
           G+VHCD+K  N++I+  G  KIADFGC++ V    E+ + GTP FMAPEVARGEKQG  S
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DIWALGCTLIEMVTGAPPWKITD---DPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCL 240
           DIWA+GCT+IEMVTG+PPW   D   DPVSVLYR+GYS E+PE+P  L+E+ KDFL KCL
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 RREATERWSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESE---- 300
           +REA ERW+A+QLL HPFL      +E +    L S SPTS+ DQ+ W S+EEE E    
Sbjct: 241 KREANERWTATQLLNHPFL-TTKPDIEPVLVPGLISNSPTSVTDQTFWRSVEEEEEEETE 300

Query: 301 -------TLLRTEQW--EDDRIERLATFSG--EIKWELGDENWITIRSYVDGE----DED 345
                   L R   W    +RI RL    G    + ++   +WI +R+  +G      + 
Sbjct: 301 EIQKDSRDLDRLSLWGCYSERIGRLKCVGGLDGTRCDMEGGDWIMVRARCEGTMISGSQK 360

BLAST of Chy1G004380 vs. TAIR 10
Match: AT1G05100.1 (mitogen-activated protein kinase kinase kinase 18 )

HSP 1 Score: 322.4 bits (825), Expect = 4.8e-88
Identity = 180/347 (51.87%), Postives = 229/347 (65.99%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGY 60
           M WTR   +G GS+ATV  A+   SG+  AVKSAE  +S+FL+ E ++LSSL++P ++GY
Sbjct: 1   MNWTRGKTLGRGSTATVSAATCHESGETLAVKSAEFHRSEFLQREAKILSSLNSPYVIGY 60

Query: 61  RGFDVTTE----NGK-LMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEY 120
           RG ++T E    NG+   Y+L MEYA  GTLTD   + GG I E     YTR+I+ GLEY
Sbjct: 61  RGCEITREPFHNNGEATTYSLLMEYAPYGTLTDVATKNGGFIDEARVVKYTRQILLGLEY 120

Query: 121 LH-KQGLVHCDIKAKNILIAGDG-LKIADFGCSRWV-CEAEAVIGGTPMFMAPEVARGEK 180
           +H  +G+ HCDIK  N+L+  +G  KIADFGC++WV  E    + GTP FMAPE ARGE+
Sbjct: 121 IHNSKGIAHCDIKGSNVLVGENGEAKIADFGCAKWVEPEITEPVRGTPAFMAPEAARGER 180

Query: 181 QGISSDIWALGCTLIEMVTGAPPWKITD--DPVSVLYRIGYSGESPEIPSFLSEKGKDFL 240
           QG  SDIWA+GCT+IEMVTG+ PW   D  DPVSVLYR+GY GE PE+P  L+E+ KDFL
Sbjct: 181 QGKESDIWAVGCTVIEMVTGSQPWIGADFTDPVSVLYRVGYLGELPELPCSLTEQAKDFL 240

Query: 241 RKCLRREATERWSASQLLEHPFL----GELSSGLEEIKELHLYSQSPTSILDQSLWNSLE 300
            KCL++EATERW+ASQLL HPFL     EL +G        L + SPTS+ DQ  W S+E
Sbjct: 241 GKCLKKEATERWTASQLLNHPFLVNKEPELVTG--------LVTNSPTSVTDQMFWRSVE 300

Query: 301 EE-SETLLRTEQWEDDRIERLATFSG------EIKWELGDENWITIR 327
           EE SE   R+  WE    ER+   S       E  W+L  E+WIT+R
Sbjct: 301 EEVSED--RSSWWECHEDERIGVLSWIGHVVVESTWDLDGEDWITVR 337

BLAST of Chy1G004380 vs. TAIR 10
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15 )

HSP 1 Score: 320.1 bits (819), Expect = 2.4e-87
Identity = 173/368 (47.01%), Postives = 235/368 (63.86%), Query Frame = 0

Query: 3   WTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGYRG 62
           W R   +G GS+ATV L  T +SG  FAVKSAE   S FL+ EQ +LS LS+P IV Y G
Sbjct: 6   WIRGPIIGRGSTATVSLGIT-NSGDFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYIG 65

Query: 63  FDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQGLV 122
            +VT EN KLMYNL MEY +GG+L D I   GG++ EP    YTR+I++GL YLH QG+V
Sbjct: 66  SNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQGIV 125

Query: 123 HCDIKAKNILIAGDGLKIADFGCSRWVCEAEAV-IGGTPMFMAPEVARGEKQGISSDIWA 182
           HCD+K++N++I G+  KI D GC++ V E E +   GTP FM+PEVARGE+Q   +D+WA
Sbjct: 126 HCDVKSQNVMIGGEIAKIVDLGCAKTVEENENLEFSGTPAFMSPEVARGEEQSFPADVWA 185

Query: 183 LGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATER 242
           LGCT+IEM TG+ PW   +D V+ +Y+IG++GESP IP +LSEKG+DFLRKCLR++  +R
Sbjct: 186 LGCTVIEMATGSSPWPELNDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDPKQR 245

Query: 243 WSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQ---- 302
           W+  +LL+HPFL E  +  ++     L S SP+++LDQ  W+  E      ++ +     
Sbjct: 246 WTVEELLQHPFLDEEDNDSDQTGNC-LNSSSPSTVLDQRFWDLCETSRSRFIKEDHEDPF 305

Query: 303 -------WED-----DRIERLA--TFSGEIKWELGDENWITIRSYVD--GEDEDELSFSR 350
                  W+D     DRI++LA    SGE  WE     WI +R  ++   E+EDE     
Sbjct: 306 ANSTNFLWDDDSLPGDRIKKLAGDESSGEPDWE--TNGWIEVRGEIEKRNEEEDENCVEA 365

BLAST of Chy1G004380 vs. TAIR 10
Match: AT4G26890.1 (mitogen-activated protein kinase kinase kinase 16 )

HSP 1 Score: 300.4 bits (768), Expect = 2.0e-81
Identity = 165/376 (43.88%), Postives = 239/376 (63.56%), Query Frame = 0

Query: 3   WTRRSPVGNGSSATVYLASTASSGQLFAVKSAELLKSDFLKIEQRVLSSLSNPSIVGYRG 62
           WTR   +G GS+ATV +A  +SSG+LFAVKSA+L  S  L+ EQ +LS+LS+P +V Y G
Sbjct: 5   WTRGPIIGRGSTATVSIA-ISSSGELFAVKSADLSSSSLLQKEQSILSTLSSPHMVKYIG 64

Query: 63  FDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLEYLHKQGLV 122
             +T E+  L+YN+ MEY +GG L D I   GG++ EP    YTR+I+ GL YLH++G+V
Sbjct: 65  TGLTRESNGLVYNILMEYVSGGNLHDLIKNSGGKLPEPEIRSYTRQILNGLVYLHERGIV 124

Query: 123 HCDIKAKNILIAGDG-LKIADFGCSRWVCEAEAVIGGTPMFMAPEVARGEKQGISSDIWA 182
           HCD+K+ N+L+  +G LKIAD GC++ V ++E    GTP FMAPEVARGE+Q   +D+WA
Sbjct: 125 HCDLKSHNVLVEENGVLKIADMGCAKSVDKSE--FSGTPAFMAPEVARGEEQRFPADVWA 184

Query: 183 LGCTLIEMVTGAPPWKITDDPVSVLYRIGYSGESPEIPSFLSEKGKDFLRKCLRREATER 242
           LGCT+IEM+TG+ PW   +D V+ +Y+IG+SGESP IP+++S+K KDFL+ CL+ +  +R
Sbjct: 185 LGCTMIEMMTGSSPWPELNDVVAAMYKIGFSGESPAIPAWISDKAKDFLKNCLKEDQKQR 244

Query: 243 WSASQLLEHPFLGELSSGLEEIKELHLYSQSPTSILDQSLWNSLEEESETLLRTEQWED- 302
           W+  +LL+HPFL +     +    L   + SP+++LDQ  W+S E     L+  +  +  
Sbjct: 245 WTVEELLKHPFLDD-DEESQTSDCLKNKTSSPSTVLDQRFWDSCESSKSHLVSIDHEDPF 304

Query: 303 -----------DRIERLA--TFSGEIKWELGDE-NWITIRSYVDGEDEDELSFSRNDLDL 362
                      DRIE+LA   FS  + W+  D+  WI +R    GE   E      D D+
Sbjct: 305 AEYSESLDSPADRIEKLAGDEFSSLLDWDTEDDGGWIQVR----GEKHKETEKRDGDEDV 364

BLAST of Chy1G004380 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 213.8 bits (543), Expect = 2.4e-55
Identity = 130/299 (43.48%), Postives = 178/299 (59.53%), Query Frame = 0

Query: 1   MAWTRRSPVGNGSSATVYLASTASSG-----QLFAVKSAELLKSDFLKIEQRVLSSLSNP 60
           M W RR  +G+GS +TV LA+T+ S       L AVKS+ ++ S  L+ E+ VL  L + 
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 61  S-IVGYRGFDVTTENGKLMYNLFMEYAAGGTLTDEIFRRGGRIKEPTAAFYTREIVRGLE 120
           S IV   G   T ENG+ +YNLF+EYA+GG+L D I   G  + E     +TR IV+GL 
Sbjct: 61  SEIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGLC 120

Query: 121 YLHKQGLVHCDIKAKNILIAGDG-LKIADFG-CSRWVCEAEAVIGGTPMFMAPEVARGEK 180
           ++H  G  HCDIK +N+L+ GDG +KI+DFG   R   E    I GTP++MAPE     +
Sbjct: 121 HIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKRRSGEVCVEIRGTPLYMAPESVNHGE 180

Query: 181 QGISSDIWALGCTLIEMVTGAPPWKITD----DPVSVLYRIGYSGESPEIPSFLSEKGKD 240
               +DIWALGC+++EM +G   W + D    + +S+L RIG   E P IP  LSE+GKD
Sbjct: 181 FESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKD 240

Query: 241 FLRKCLRREATERWSASQLLEHPFLG-ELSSGLEEIKELHLYSQSPTSILDQSLWNSLE 287
           F+ KC  + A ERW+A  LL+HPFL  +  SG E+       S SP +  D   WNS++
Sbjct: 241 FVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEED----EACSVSPRNPFDFPGWNSVQ 295

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O808882.9e-9049.59Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370... [more]
Q9ZVP56.8e-8751.87Mitogen-activated protein kinase kinase kinase 18 OS=Arabidopsis thaliana OX=370... [more]
Q9SND61.7e-5036.39Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370... [more]
Q405418.9e-4739.93Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum OX=4097... [more]
O220423.7e-4538.43Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702... [more]
Match NameE-valueIdentityDescription
A0A0A0K6U11.2e-20697.31Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G043... [more]
A0A5A7T9T32.0e-19893.39Mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3C1N11.7e-19793.12mitogen-activated protein kinase kinase kinase 2 OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1GLZ47.1e-14878.08mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=... [more]
A0A6J1HZ761.0e-14677.78mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36... [more]
Match NameE-valueIdentityDescription
XP_004144594.12.93e-26297.31mitogen-activated protein kinase kinase kinase 18 [Cucumis sativus] >KGN43511.1 ... [more]
KAA0040234.12.43e-25193.39mitogen-activated protein kinase kinase kinase 2 [Cucumis melo var. makuwa] >TYK... [more]
XP_008455448.14.02e-25093.12PREDICTED: mitogen-activated protein kinase kinase kinase 2 [Cucumis melo][more]
XP_038887174.12.69e-21580.05mitogen-activated protein kinase kinase kinase 18 [Benincasa hispida][more]
KAG6572287.11.00e-18679.33Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper... [more]
Match NameE-valueIdentityDescription
AT2G32510.12.1e-9149.59mitogen-activated protein kinase kinase kinase 17 [more]
AT1G05100.14.8e-8851.87mitogen-activated protein kinase kinase kinase 18 [more]
AT5G55090.12.4e-8747.01mitogen-activated protein kinase kinase kinase 15 [more]
AT4G26890.12.0e-8143.88mitogen-activated protein kinase kinase kinase 16 [more]
AT4G36950.12.4e-5543.48mitogen-activated protein kinase kinase kinase 21 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 3..253
e-value: 9.0E-69
score: 244.5
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 5..253
e-value: 1.6E-53
score: 181.8
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 3..253
score: 40.979427
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 2..313
e-value: 4.7E-67
score: 228.5
NoneNo IPR availablePIRSRPIRSR037921-1PIRSR037921-1coord: 76..210
e-value: 1.3E-8
score: 32.4
NoneNo IPR availablePANTHERPTHR48011CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATEDcoord: 1..336
NoneNo IPR availablePANTHERPTHR48011:SF76MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 15coord: 1..336
NoneNo IPR availableCDDcd06606STKc_MAPKKKcoord: 3..253
e-value: 1.87592E-124
score: 357.216
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 121..133
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 9..32
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 5..297

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G004380.1Chy1G004380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity