Chy1G002340 (gene) Cucumber (hystrix) v1

Overview
NameChy1G002340
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
DescriptionAldolase-type TIM barrel family protein
LocationchrH01: 1622223 .. 1625901 (+)
RNA-Seq ExpressionChy1G002340
SyntenyChy1G002340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTTGGGGGCTTACTTGGTTTTCAATATGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCGGTCCCGAGCTCGTTGCTGCCGTTGCCAATGCCGGTGGACTTGGTCTTCTCCGAGCCCCCGATTGGGTACTTCACTGTTTATTGTTTTTTCTTGTTTTTTTTTTTCCTTTACCCATCTTGGAAATGAATGTAATGTGTTGTGGAGAAGTATCTCTGATGTGCGATACGTGAGTCGAAACGGAGTTTCTTCTTATTTTGTTGATTGATTGTTCGATCATACGTATACGGATTTTAAAACTTTTAATTCTTGATCGATTTGAATGTTTGGCGTTTTAGTTTTTGCAACTGCTTGTTGCATGGTTTTATAGGAATTCGGACGATTTTGGTGATGATTGGTTATGGAATTATGGAGGAGTGTTGGGTTTAACTGGAGACGTAGTCGGGGGAATTGATAATGTCAATGAAGAGTTGGATCATTGATCTTGTCAATCTGATATGAACAGTTTGTATATGATATGAATGTAAAGAGTTTTCTTAAAACTATGGATATAATTAAATGTACCATAACCTTTTCTGTAAATTGGTGATTTAACTTACTCATTTCTATCTAATTAATATTTATTTCCATTTGTTGTGCTCTCAAGTGCGGATGTATGGATATAATTAAAATTATTGTTTAGGATTGATAAGTGATGCAAGCTTCTTACAGCTATTTTCTTCCTTCATTTGTTCTTCTATTAGTTAGGAAATTAGTTGTAAAATTTTATAACTATTTTGTCTTTTTTTTTGTCTAATAGCTTGTGTATGCAGTATTTAGCTTTAGTTTATATTTTCTGCAATCGTTTTGAGATTTTTGCCAAAATTTGACCAATCAATCAATTTTGTTTCGAGAATTACATGTTTTTGCTTTCTGAACCTTGCTATGATCTCAAAAGTATTTATAGGCATGTATTAAGGGCTGAACAGAGTTATATTCTTAAATTGGCAACATACAGTAAACTGATTATCAAACAGTTTTTTTATTATAAAGTTAAAAGGTGTATATCTTCCCATTTATCTCAACTGTTTATCTACATCATTCCTATTCTCCACTCTAATTGAGTAGCAAGGAGGATACGCTTTAAAATTTTATGTGTACCAGGAGTCACCTGATTATGTACGAGAATTGATAAGGAAGACCCGAGCTTTAACGGATAAACCATTTGGAATAGGTGTTATTTTAGCATTTCCTCACGAGCAAAATCTAAAGGCCATATTAGACGAGAAAGTAGCTGTTGTGCAAGTTTACTGGGGAGAATGTTCGAAGGATCTTGTGGATCAAGTTCATTCTGCTGGGGTGAAAATTATTCCACAAGTGAGTATTCTCTTGCAAATCATGTTAACAATCCATTTCCTAAGCATCAAATCAAGTCTGATAGGTTAATCTCATCATTAAACTTTTTATGCATGGTTGTGGTGGCTTAATTTCTATAAAAACATTTTGTTTATTGGAAAAAAATTGTGCATGGACGTAGAACTTGGAATTATAGTCTGAAATTACGTGCTTATCTTGTTTTCTAGTCTATCACTTCAGGCTTCATTATTCTGTTCTAGGATGTTATTGGTTTTCTACTCATATTGTGGCATGTTACAACTTAAAAATACTTTTGCAGGTTGGGAGTGTTGAAGAAGCAAGTAAAGCAGTTGATGTTGGCGTTGATGCAATAATTGTGCAAGGTCGGGAAGCAGGAGGGCATGTTATTGGTCAAGTATAATCTTCTTCTTCCCATTAATTTCATTAATTCATGATGCCATTGTATTTGTTTCCAACTTTTCTACTGTTTCCATTATAGGGTTTTTCCCATCCTCTTAATCACTTGGAGGCTATGTTTTTGTCCGGTTCTTCTCCTTAGTAAAAATTCTCTAAATTACTAGAAAAGAAATAAATTAACAAGGAAAGATAATTTGACTTATATTAGAAAGCTAATTTCATCAAACATCTGTTTCTCAATTCATTAAGAGGCTTTTGTCTTAGTTCAGGTACCCACATACTTACCCCGCTCCAACTTCACCTTTTGAAGAATATTCAACTTTGGTTTTGTTGAGACAATGTTTACGTTAGAAGCATGTATTCAGTTTGAAAATCACCTTTGGAGAGAAGCAGAATTTAAGAAATTGATCTTTATCATATAAGTATTTGAGCAGTCTTCCTTTTTCACAATCATGCATGCGTATACGCTTTAAGTAATGCCTACTTTTCTTCTTTAAATTTTATACTGCTGGTTTTTTATTTGCTGCGCCCAGTAAGTGATATTACTTTGGTTCATGTTTCATTAGTCATATTCTTCATCTTTCTTCACATTTGTATTCCAACTATATCATAACTTATCTCAAATATATATCTATTTCATTATTGGAAAAGATTGAAATCGTCTACAGAAAATTGACTTGGTTTTTTACAGGAAGGTTTGATTTCCATCTTACCAAGAGTAGTTGAAATTGCTGGGGATAAAGATATACCTGTAATTGCGGCTGGCAGTATTTCAGATTCCCGAGGTTATGTTGCTGCCCTGGCCCTTGGCGCTCATGGTGTCTGCCTTGGAACTAGGTTAGAAAACCTCAACTGTGACCATTCACAACTTGAAAACTTATTTTAAAAAGCTGGTAGATCCATGCTACAAAATATCGCTCAATTTGAAATAATTTCCAGCATTAAAGTAGAACGTTGACACTTTTCGGTTAGGTTTGGTTCGGAGTCAATATGCAAGTATGATAAGAATGCCAATCCAGAAACTTTGTTATCGGTCCATGACTTGCATTCTTAAAGATGTGTCTAATTTTCGAACATCGACACCATGAAAATATAGCCATGAGAATGGTGTTGGAGGAAGGTTTAAGGTTGAATGGCCATATCATAATCCATCTACTAAATAGTTCAGAGGCAGAAAACCTGTCTTTCTTTGGTGTAATCCAAAAGTTGCCGTTATTTTTATTATATTATTGTAATTGGAACGATTGTATAATCTAATAACGAAACAAATCTCATAAATTTTAGGTAATGCAACAGTTGATCTTTTACATCCTCATAATGAGTTAAATGGAATATTGCAGATTTGTTGCTACTGAAGAAAGCAATGCTCACCCTACATACAAGAGGAAGTTGGTGGAACTGGAAGCAACTGATTATACAAACATTTTCGGCCGTGCAAGATGGCCTGGTGCGCCACAGCGTGTTCTGCAGACTCCCTTTTACGATGATTGGAAGTCACTTCCTGCTAATGAAAATGAAAGCAACCAGCCTGTCATCGGGCGCTCAACAATTAACGGCTTGGTGAGCTCCTCCTTGATCTCTTTTCTACTTAAACATGTGCACATGCTTGAGAACAAATTCTGCAATTAGTTTGTATGCTGGATCATTACAAGATCAAATATTAGGTTGAGGGTATTCTGTTCTGCTCTAATCTAAATCAATCCTTCGTTCTTGGTACAGGACATAGATATACGGCGCTTTGCGGGTACTGTACCAAATGCAACGACAAAAGGTGACATTGAGAGCATGGCAATGTATGCAGGGCAAGGAGTAGGACTGATCAAGGAGATTCTACCAGCGGGAGAAGTGGTAAGGAGGCTGGTGGAAGGTGCTCAACATTTGATCCAAACACATCTTAGTAGTTTTGTTCATAAAGATTGA

mRNA sequence

ATGGGTTTTGGGGGCTTACTTGGTTTTCAATATGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCGGTCCCGAGCTCGTTGCTGCCGTTGCCAATGCCGGTGGACTTGGTCTTCTCCGAGCCCCCGATTGGGAGTCACCTGATTATGTACGAGAATTGATAAGGAAGACCCGAGCTTTAACGGATAAACCATTTGGAATAGGTGTTATTTTAGCATTTCCTCACGAGCAAAATCTAAAGGCCATATTAGACGAGAAAGTAGCTGTTGTGCAAGTTTACTGGGGAGAATGTTCGAAGGATCTTGTGGATCAAGTTCATTCTGCTGGGGTGAAAATTATTCCACAAGTTGGGAGTGTTGAAGAAGCAAGTAAAGCAGTTGATGTTGGCGTTGATGCAATAATTGTGCAAGGTCGGGAAGCAGGAGGGCATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGAGTAGTTGAAATTGCTGGGGATAAAGATATACCTGTAATTGCGGCTGGCAGTATTTCAGATTCCCGAGGTTATGTTGCTGCCCTGGCCCTTGGCGCTCATGGTGTCTGCCTTGGAACTAGATTTGTTGCTACTGAAGAAAGCAATGCTCACCCTACATACAAGAGGAAGTTGGTGGAACTGGAAGCAACTGATTATACAAACATTTTCGGCCGTGCAAGATGGCCTGGTGCGCCACAGCGTGTTCTGCAGACTCCCTTTTACGATGATTGGAAGTCACTTCCTGCTAATGAAAATGAAAGCAACCAGCCTGTCATCGGGCGCTCAACAATTAACGGCTTGGACATAGATATACGGCGCTTTGCGGGTACTGTACCAAATGCAACGACAAAAGGTGACATTGAGAGCATGGCAATGTATGCAGGGCAAGGAGTAGGACTGATCAAGGAGATTCTACCAGCGGGAGAAGTGGTAAGGAGGCTGGTGGAAGGTGCTCAACATTTGATCCAAACACATCTTAGTAGTTTTGTTCATAAAGATTGA

Coding sequence (CDS)

ATGGGTTTTGGGGGCTTACTTGGTTTTCAATATGGCATAGTTCAGGCTCCGCTTGGACCTGATATCTCCGGTCCCGAGCTCGTTGCTGCCGTTGCCAATGCCGGTGGACTTGGTCTTCTCCGAGCCCCCGATTGGGAGTCACCTGATTATGTACGAGAATTGATAAGGAAGACCCGAGCTTTAACGGATAAACCATTTGGAATAGGTGTTATTTTAGCATTTCCTCACGAGCAAAATCTAAAGGCCATATTAGACGAGAAAGTAGCTGTTGTGCAAGTTTACTGGGGAGAATGTTCGAAGGATCTTGTGGATCAAGTTCATTCTGCTGGGGTGAAAATTATTCCACAAGTTGGGAGTGTTGAAGAAGCAAGTAAAGCAGTTGATGTTGGCGTTGATGCAATAATTGTGCAAGGTCGGGAAGCAGGAGGGCATGTTATTGGTCAAGAAGGTTTGATTTCCATCTTACCAAGAGTAGTTGAAATTGCTGGGGATAAAGATATACCTGTAATTGCGGCTGGCAGTATTTCAGATTCCCGAGGTTATGTTGCTGCCCTGGCCCTTGGCGCTCATGGTGTCTGCCTTGGAACTAGATTTGTTGCTACTGAAGAAAGCAATGCTCACCCTACATACAAGAGGAAGTTGGTGGAACTGGAAGCAACTGATTATACAAACATTTTCGGCCGTGCAAGATGGCCTGGTGCGCCACAGCGTGTTCTGCAGACTCCCTTTTACGATGATTGGAAGTCACTTCCTGCTAATGAAAATGAAAGCAACCAGCCTGTCATCGGGCGCTCAACAATTAACGGCTTGGACATAGATATACGGCGCTTTGCGGGTACTGTACCAAATGCAACGACAAAAGGTGACATTGAGAGCATGGCAATGTATGCAGGGCAAGGAGTAGGACTGATCAAGGAGATTCTACCAGCGGGAGAAGTGGTAAGGAGGCTGGTGGAAGGTGCTCAACATTTGATCCAAACACATCTTAGTAGTTTTGTTCATAAAGATTGA

Protein sequence

MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRGYVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD*
Homology
BLAST of Chy1G002340 vs. ExPASy Swiss-Prot
Match: Q0S7Q1 ((3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA dehydrogenase OS=Rhodococcus jostii (strain RHA1) OX=101510 GN=ipdC PE=3 SV=1)

HSP 1 Score: 141.0 bits (354), Expect = 2.5e-32
Identity = 93/239 (38.91%), Postives = 131/239 (54.81%), Query Frame = 0

Query: 6   LLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 65
           L+G Q+ +VQ  +G  ++GP LVA  ANAGGLG+L +      + +   I KT+ LTDKP
Sbjct: 11  LVGVQHPVVQTGMG-WVAGPRLVAGTANAGGLGILASATMTYTE-LESAITKTKTLTDKP 70

Query: 66  FGIGV---ILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEE 125
           FG+ +       PH  +L  ++ E V V         ++L+ ++ +AGV +IP +G+ + 
Sbjct: 71  FGVNIRADASDAPHRIDL--LIRESVRVASFALAP-KQELIAKLKAAGVVVIPSIGAAKH 130

Query: 126 ASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRGYV 185
           A K    G DA+IVQG E GGH  G      +LP V++     DIPV+AAG   D RG  
Sbjct: 131 AKKVASWGADAVIVQGGEGGGHT-GPVATTLLLPSVLDAV---DIPVVAAGGFYDGRGLA 190

Query: 186 AALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQT 242
           AALA GA GV +GTRF+ T+ES    + K + +     D T      +  G P RVL T
Sbjct: 191 AALAYGAAGVAMGTRFLLTQESTVPDSVKHEYLARGLQDTT---VSRKVDGMPHRVLNT 237

BLAST of Chy1G002340 vs. ExPASy Swiss-Prot
Match: P71847 ((3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA dehydrogenase OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=ipdC PE=1 SV=3)

HSP 1 Score: 135.6 bits (340), Expect = 1.1e-30
Identity = 91/247 (36.84%), Postives = 132/247 (53.44%), Query Frame = 0

Query: 6   LLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 65
           L+G ++ +VQ  +G  ++G  LV+A ANAGGLG+L A    + D +   I K +A+TDKP
Sbjct: 10  LIGIEHPVVQTGMG-WVAGARLVSATANAGGLGIL-ASATMTLDELAAAITKVKAVTDKP 69

Query: 66  FGIGVIL-AFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEAS 125
           FG+ +   A      ++ ++ E V V         + L+ ++  AG  +IP +G+ + A 
Sbjct: 70  FGVNIRADAADAGDRVELMIREGVRVASFALAP-KQQLIARLKEAGAVVIPSIGAAKHAR 129

Query: 126 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRGYVAA 185
           K    G DA+IVQG E GGH  G      +LP V++      IPVIAAG   D RG  AA
Sbjct: 130 KVAAWGADAMIVQGGEGGGHT-GPVATTLLLPSVLDAVAGTGIPVIAAGGFFDGRGLAAA 189

Query: 186 LALGAHGVCLGTRFVATEESNAHPTYKRKLVE--LEATDYTNIFGRARWPGAPQRVLQTP 245
           L  GA GV +GTRF+ T +S      KR+ ++  L+ T  T      R  G P RVL+T 
Sbjct: 190 LCYGAAGVAMGTRFLLTSDSTVPDAVKRRYLQAGLDGTVVT-----TRVDGMPHRVLRTE 247

Query: 246 FYDDWKS 250
             +  +S
Sbjct: 250 LVEKLES 247

BLAST of Chy1G002340 vs. ExPASy Swiss-Prot
Match: B2TEK6 (Nitronate monooxygenase OS=Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN) OX=398527 GN=Bphyt_4144 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 5.8e-29
Identity = 115/358 (32.12%), Postives = 162/358 (45.25%), Query Frame = 0

Query: 6   LLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKP 65
           LLG    I+QAP+   +S P L AAV NAGGLG L      + D  R++IR+TRALTDKP
Sbjct: 13  LLGIDKPIIQAPMA-GVSTPALAAAVCNAGGLGSLGVGAMNA-DGARKVIRETRALTDKP 72

Query: 66  FGIGVILAFPHEQN---------------------------------------LKAILDE 125
           F I V    P + +                                       L   L+E
Sbjct: 73  FNINVFCHRPAQADAAVEQQWLSWLAPHFEKYGATPPAKLSDIYTSFLADPAMLAVFLEE 132

Query: 126 KVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGGH-- 185
           K A+V  ++G  S D++ ++  AG++++    +++EA++    GVDAI+ QG EAGGH  
Sbjct: 133 KPAIVSFHFGLPSADVIAELKKAGIRLLASATNLQEAAQVEAAGVDAIVAQGIEAGGHRG 192

Query: 186 VIGQEGL---ISILPRVVEIAGDKDIPVIAAGSISDSRGYVAALALGAHGVCLGTRFVAT 245
           V   +     +        +A +  +PVIAAG I D  G  AALALGA    LGT FVA 
Sbjct: 193 VFDPDAFDDRLGTFALTRLLAKECRLPVIAAGGIMDGAGIAAALALGAQAAQLGTAFVAC 252

Query: 246 EESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPV 305
            E++    Y+R L+   A        R  +  A    L     + + +L A +  S +P 
Sbjct: 253 TETSIDEGYRRALLGEAAR-------RTTFTAAISGRLARSMANSFTALGA-DPRSPEPA 312

Query: 306 IGRSTINGLDIDIRRFAGTVPNAT--TKGDIESMAMYAGQGVGLIKEILPAGEVVRRL 318
                       I   AG   NA    KG+    A +AGQ   L +  LPA E+V +L
Sbjct: 313 ---------TYPIAYDAGKALNAAAKAKGEFGYGAHWAGQAAALARS-LPAAELVAQL 350

BLAST of Chy1G002340 vs. ExPASy Swiss-Prot
Match: Q9HWH9 (Nitronate monooxygenase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=nmoA PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.6e-29
Identity = 115/363 (31.68%), Postives = 163/363 (44.90%), Query Frame = 0

Query: 3   FGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALT 62
           F  LLG Q  I+QAP+   +S P L AAV+NAGGLG + A    + +  R LIR+ R LT
Sbjct: 5   FTRLLGIQQPIIQAPM-LGVSTPALAAAVSNAGGLGSI-AITGSAAEKGRALIREVRGLT 64

Query: 63  DKPFGIGVILAFPHEQN---------------------------------------LKAI 122
           DKPF + +    P + +                                       L  +
Sbjct: 65  DKPFNVNLFCHRPGQADPARERAWLDYLKPLFAEFGAEPPVRLKNIYLSFLEDPTLLPML 124

Query: 123 LDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGG 182
           L+E+ A V  ++G   +D V  + + G++++    + EEA+     G DA++ QG EAGG
Sbjct: 125 LEERPAAVSFHFGAPPRDQVRALQAVGIRVLVCATTPEEAALVEAAGADAVVAQGIEAGG 184

Query: 183 H--VIGQE---GLISILPRVVEIAGDKDIPVIAAGSISDSRGYVAALALGAHGVCLGTRF 242
           H  V   E     I  L  V  +A    +PV+AAG I D RG  AAL LGA  V +GT F
Sbjct: 185 HRGVFEPERGDAAIGTLALVRLLAARGSLPVVAAGGIMDGRGIRAALELGASAVQMGTAF 244

Query: 243 VATEESNAHPTYKRKLVELEA--TDYTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENE 302
           V   ES+A+  Y+  L    A  T  T         G P R+    F+D   + P     
Sbjct: 245 VLCPESSANAAYREALKGPRAARTALTVTMSGRSARGLPNRM----FFD--AAAPGVPPL 304

Query: 303 SNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRR 320
            + P +  +T                 A  +G+ +  A +AGQG  L +E LPA E++R 
Sbjct: 305 PDYPFVYDAT-----------KALQTAALARGNHDFAAQWAGQGAALARE-LPAAELLRT 347

BLAST of Chy1G002340 vs. ExPASy Swiss-Prot
Match: O05413 (Probable nitronate monooxygenase OS=Bacillus subtilis (strain 168) OX=224308 GN=yrpB PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 9.3e-27
Identity = 96/348 (27.59%), Postives = 159/348 (45.69%), Query Frame = 0

Query: 13  IVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDKPFGIGVIL 72
           I+QAP+   I+ P L +AV+N G LG L A  + +PD + + I++   LTD PF I V +
Sbjct: 14  IIQAPMAGGITKPRLASAVSNQGALGSL-ASGYLTPDLLEQQIKEIFELTDAPFQINVFV 73

Query: 73  AFPHE----------------------------------QNLKAILDEKVAVVQVYWGEC 132
               E                                  Q +  IL  KV      +   
Sbjct: 74  PLGLEMPPKDQIKKWKENIPLANQVNQFTSVQEEWDDFYQKIDLILKYKVKACSFTFDLP 133

Query: 133 SKDLVDQVHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGGH-----VIGQEGLIS 192
            +D V ++ +AG  +I    +VEEA    + G+D +++QG EAGGH         E  + 
Sbjct: 134 PEDAVKELKTAGCCLIGTASTVEEALLMEERGMDIVVLQGSEAGGHRGAFLPSKGESAVG 193

Query: 193 ILPRVVEIAGDKDIPVIAAGSISDSRGYVAALALGAHGVCLGTRFVATEESNAHPTYKRK 252
           ++  + + A    +PVIAAG + D RG  AAL LGA GV +G+ F+   ESNAHP +K+K
Sbjct: 194 LMALIPQAADALSVPVIAAGGMIDHRGVKAALTLGAQGVQIGSAFLICHESNAHPVHKQK 253

Query: 253 LVELEATD--YTNIFGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLD 312
           ++E    D   T +F      G   + ++     + ++LP                N L 
Sbjct: 254 ILEANEADTKLTTLFSGKEARGIVNKWMEENEQFETQTLPYPYQ------------NTLT 313

Query: 313 IDIRRFAGTVPNATTKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVE 320
             +R+       A+ + + + M+++AGQG+  + E +   +++ +L +
Sbjct: 314 KAMRQ------KASLQNNHDQMSLWAGQGIRSLTEEISVKQLLNQLCQ 342

BLAST of Chy1G002340 vs. ExPASy TrEMBL
Match: A0A0A0K422 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G064010 PE=4 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 2.4e-187
Identity = 333/336 (99.11%), Postives = 334/336 (99.40%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 55  MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 114

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 115 LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 174

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEI GDKDIPVIAAGSISDSRG
Sbjct: 175 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRG 234

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ
Sbjct: 235 YVAALALGARGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 294

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 295 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 354

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 337
           VGLIKEILPAGEVVRRLVEGAQHLI+THLSSFVHKD
Sbjct: 355 VGLIKEILPAGEVVRRLVEGAQHLIRTHLSSFVHKD 390

BLAST of Chy1G002340 vs. ExPASy TrEMBL
Match: A0A5A7SPI9 (Putative nitronate monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002230 PE=4 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 8.6e-185
Identity = 328/336 (97.62%), Postives = 332/336 (98.81%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M FGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVD GVDAIIVQGREAGGHVIGQEGLISILPRVVEI G++DIPVIAAGSISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES+AHPTYKRKLVE+EATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 337
           VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336

BLAST of Chy1G002340 vs. ExPASy TrEMBL
Match: A0A1S3C1I8 (probable nitronate monooxygenase OS=Cucumis melo OX=3656 GN=LOC103495406 PE=4 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 8.6e-185
Identity = 328/336 (97.62%), Postives = 332/336 (98.81%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M FGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVD GVDAIIVQGREAGGHVIGQEGLISILPRVVEI G++DIPVIAAGSISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES+AHPTYKRKLVE+EATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 337
           VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336

BLAST of Chy1G002340 vs. ExPASy TrEMBL
Match: A0A6J1GL38 (uncharacterized protein LOC111454946 OS=Cucurbita moschata OX=3662 GN=LOC111454946 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 3.7e-180
Identity = 316/336 (94.05%), Postives = 329/336 (97.92%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M  GG+LGF+YGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDYVRELIRKTRA
Sbjct: 1   MDSGGILGFRYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAV+QVYWGECSKDLVDQ HSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKDLVDQAHSAGVKIIPQVGSV 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVV+I G++DIP+IAAG ISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES+AHP YKRKLVEL+ATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 337
           VGLIKEILPAGEVVR+LVEGAQHLIQTHLSSFVHKD
Sbjct: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 336

BLAST of Chy1G002340 vs. ExPASy TrEMBL
Match: A0A6J1I3E9 (uncharacterized protein LOC111470639 OS=Cucurbita maxima OX=3661 GN=LOC111470639 PE=4 SV=1)

HSP 1 Score: 632.1 bits (1629), Expect = 1.3e-177
Identity = 312/335 (93.13%), Postives = 326/335 (97.31%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M  GG+LGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MDSGGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAV+QVYWGECSKDLVDQ HSAGVKIIPQVG +
Sbjct: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKDLVDQAHSAGVKIIPQVGCI 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVV+I G++DIP+IAAG ISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES+AHP YKRKLVEL+ATDYT+IFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTDIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPA+ENESNQPVIGRS INGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPADENESNQPVIGRSRINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHK 336
           VGLIKEILPAGEVVR+LVEGAQHLIQTHLSSFVHK
Sbjct: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHK 335

BLAST of Chy1G002340 vs. NCBI nr
Match: XP_031744639.1 (uncharacterized protein LOC101221123 [Cucumis sativus])

HSP 1 Score: 659 bits (1701), Expect = 1.24e-238
Identity = 333/336 (99.11%), Postives = 334/336 (99.40%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEI GDKDIPVIAAGSISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGDKDIPVIAAGSISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336
           VGLIKEILPAGEVVRRLVEGAQHLI+THLSSFVHKD
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIRTHLSSFVHKD 336

BLAST of Chy1G002340 vs. NCBI nr
Match: XP_008455173.1 (PREDICTED: probable nitronate monooxygenase [Cucumis melo] >KAA0031461.1 putative nitronate monooxygenase [Cucumis melo var. makuwa] >TYK06915.1 putative nitronate monooxygenase [Cucumis melo var. makuwa])

HSP 1 Score: 651 bits (1679), Expect = 2.80e-235
Identity = 328/336 (97.62%), Postives = 332/336 (98.81%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M FGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA
Sbjct: 1   MSFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVD GVDAIIVQGREAGGHVIGQEGLISILPRVVEI G++DIPVIAAGSISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVEIVGNEDIPVIAAGSISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES+AHPTYKRKLVE+EATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPTYKRKLVEMEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336
           VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336

BLAST of Chy1G002340 vs. NCBI nr
Match: XP_022952200.1 (uncharacterized protein LOC111454946 [Cucurbita moschata] >KAG7011538.1 yrpB [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 635 bits (1639), Expect = 3.50e-229
Identity = 316/336 (94.05%), Postives = 329/336 (97.92%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M  GG+LGF+YGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDYVRELIRKTRA
Sbjct: 1   MDSGGILGFRYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAV+QVYWGECSKDLVDQ HSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKDLVDQAHSAGVKIIPQVGSV 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVV+I G++DIP+IAAG ISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES+AHP YKRKLVEL+ATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336
           VGLIKEILPAGEVVR+LVEGAQHLIQTHLSSFVHKD
Sbjct: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 336

BLAST of Chy1G002340 vs. NCBI nr
Match: XP_023511402.1 (uncharacterized protein LOC111776237 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634 bits (1635), Expect = 1.42e-228
Identity = 316/336 (94.05%), Postives = 328/336 (97.62%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M  GG+LGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLR+PDWESPDYVRELIRKTRA
Sbjct: 1   MDSGGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRSPDWESPDYVRELIRKTRA 60

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDKPFG+GVILAFPHEQNLKAILDEKVAV+QVYWGECSK LVDQ HSAGVKIIPQVGSV
Sbjct: 61  LTDKPFGVGVILAFPHEQNLKAILDEKVAVLQVYWGECSKALVDQAHSAGVKIIPQVGSV 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVV+I G++DIP+IAAG ISDSRG
Sbjct: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPIIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES+AHP YKRKLVEL+ATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESHAHPAYKRKLVELDATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLDID+RRFAGTVPNATTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDVRRFAGTVPNATTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336
           VGLIKEILPAGEVVR+LVEGAQHLIQTHLSSFVHKD
Sbjct: 301 VGLIKEILPAGEVVRKLVEGAQHLIQTHLSSFVHKD 336

BLAST of Chy1G002340 vs. NCBI nr
Match: XP_038888874.1 (nitronate monooxygenase isoform X1 [Benincasa hispida])

HSP 1 Score: 633 bits (1632), Expect = 4.08e-228
Identity = 318/336 (94.64%), Postives = 325/336 (96.73%), Query Frame = 0

Query: 1   MGFGGLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRA 60
           M F G+LGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPD+VR LIRKTRA
Sbjct: 1   MDFRGILGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDFVRGLIRKTRA 60

Query: 61  LTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120
           LTDK FG+GVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV
Sbjct: 61  LTDKTFGVGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSV 120

Query: 121 EEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRG 180
           EEASKAVD GVDAIIVQGREAGGHVIGQEGLISILPRVV+I G++DIPVIAAG ISDSRG
Sbjct: 121 EEASKAVDAGVDAIIVQGREAGGHVIGQEGLISILPRVVDIVGNEDIPVIAAGGISDSRG 180

Query: 181 YVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240
           YVAALALGA GVCLGTRFVATEES AHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ
Sbjct: 181 YVAALALGARGVCLGTRFVATEESRAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQ 240

Query: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQG 300
           TPFYDDWKSLPANENESNQPVIGRSTINGLD+DIRRFAGTVPN TTKGDIESMAMYAGQG
Sbjct: 241 TPFYDDWKSLPANENESNQPVIGRSTINGLDVDIRRFAGTVPNETTKGDIESMAMYAGQG 300

Query: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHLSSFVHKD 336
           VGLIKEILPAGEVVRRLVEGAQHLIQTH SSFVHKD
Sbjct: 301 VGLIKEILPAGEVVRRLVEGAQHLIQTHFSSFVHKD 336

BLAST of Chy1G002340 vs. TAIR 10
Match: AT5G64250.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 527.7 bits (1358), Expect = 6.8e-150
Identity = 251/321 (78.19%), Postives = 292/321 (90.97%), Query Frame = 0

Query: 5   GLLGFQYGIVQAPLGPDISGPELVAAVANAGGLGLLRAPDWESPDYVRELIRKTRALTDK 64
           G+LGF+YGIVQAPLGPDISGPELVAAVANAGG+GLLR PDWE PDY+RELIRKT+ LT+K
Sbjct: 3   GILGFEYGIVQAPLGPDISGPELVAAVANAGGIGLLRCPDWECPDYLRELIRKTKTLTEK 62

Query: 65  PFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQVHSAGVKIIPQVGSVEEAS 124
           PFGIGV+LAFPH+ N+K IL+EKVAV+Q+YWG+CS++LVD  H AGVK+IPQVG+VEEA 
Sbjct: 63  PFGIGVVLAFPHDLNIKTILEEKVAVLQLYWGDCSQELVDDAHFAGVKVIPQVGNVEEAR 122

Query: 125 KAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDKDIPVIAAGSISDSRGYVAA 184
           KAV VGVDAIIVQGREAGGHVIG++GL S+LPRVV++ G++DIPVIAAG I D RGYVAA
Sbjct: 123 KAVAVGVDAIIVQGREAGGHVIGKDGLFSLLPRVVDLVGERDIPVIAAGGIVDGRGYVAA 182

Query: 185 LALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNIFGRARWPGAPQRVLQTPFY 244
           L+LGA GVCLGTRFVAT ES AHP YKRKL+E E T+YT++FGRARWPGAPQRVL+TPF+
Sbjct: 183 LSLGAQGVCLGTRFVATHESYAHPIYKRKLIEYEKTEYTDVFGRARWPGAPQRVLETPFF 242

Query: 245 DDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNATTKGDIESMAMYAGQGVGLI 304
           DDW+SLPA+ENE NQP+IGRSTI+G++ +IRRF+GTVPN TT GD+ESMAMYAGQ VGLI
Sbjct: 243 DDWRSLPAHENEVNQPIIGRSTIHGVEKEIRRFSGTVPNMTTTGDLESMAMYAGQSVGLI 302

Query: 305 KEILPAGEVVRRLVEGAQHLI 326
           KEILPAGEVV+ LVE AQ LI
Sbjct: 303 KEILPAGEVVKSLVEEAQALI 323

BLAST of Chy1G002340 vs. TAIR 10
Match: AT5G64250.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 449.5 bits (1155), Expect = 2.4e-126
Identity = 214/280 (76.43%), Postives = 252/280 (90.00%), Query Frame = 0

Query: 46  ESPDYVRELIRKTRALTDKPFGIGVILAFPHEQNLKAILDEKVAVVQVYWGECSKDLVDQ 105
           E PDY+RELIRKT+ LT+KPFGIGV+LAFPH+ N+K IL+EKVAV+Q+YWG+CS++LVD 
Sbjct: 4   ECPDYLRELIRKTKTLTEKPFGIGVVLAFPHDLNIKTILEEKVAVLQLYWGDCSQELVDD 63

Query: 106 VHSAGVKIIPQVGSVEEASKAVDVGVDAIIVQGREAGGHVIGQEGLISILPRVVEIAGDK 165
            H AGVK+IPQVG+VEEA KAV VGVDAIIVQGREAGGHVIG++GL S+LPRVV++ G++
Sbjct: 64  AHFAGVKVIPQVGNVEEARKAVAVGVDAIIVQGREAGGHVIGKDGLFSLLPRVVDLVGER 123

Query: 166 DIPVIAAGSISDSRGYVAALALGAHGVCLGTRFVATEESNAHPTYKRKLVELEATDYTNI 225
           DIPVIAAG I D RGYVAAL+LGA GVCLGTRFVAT ES AHP YKRKL+E E T+YT++
Sbjct: 124 DIPVIAAGGIVDGRGYVAALSLGAQGVCLGTRFVATHESYAHPIYKRKLIEYEKTEYTDV 183

Query: 226 FGRARWPGAPQRVLQTPFYDDWKSLPANENESNQPVIGRSTINGLDIDIRRFAGTVPNAT 285
           FGRARWPGAPQRVL+TPF+DDW+SLPA+ENE NQP+IGRSTI+G++ +IRRF+GTVPN T
Sbjct: 184 FGRARWPGAPQRVLETPFFDDWRSLPAHENEVNQPIIGRSTIHGVEKEIRRFSGTVPNMT 243

Query: 286 TKGDIESMAMYAGQGVGLIKEILPAGEVVRRLVEGAQHLI 326
           T GD+ESMAMYAGQ VGLIKEILPAGEVV+ LVE AQ LI
Sbjct: 244 TTGDLESMAMYAGQSVGLIKEILPAGEVVKSLVEEAQALI 283

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q0S7Q12.5e-3238.91(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA deh... [more]
P718471.1e-3036.84(3aS,4S,5R,7aS)-5-hydroxy-7a-methyl-1-oxo-octahydro-1H-indene-4-carboxyl-CoA deh... [more]
B2TEK65.8e-2932.12Nitronate monooxygenase OS=Paraburkholderia phytofirmans (strain DSM 17436 / LMG... [more]
Q9HWH97.6e-2931.68Nitronate monooxygenase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644... [more]
O054139.3e-2727.59Probable nitronate monooxygenase OS=Bacillus subtilis (strain 168) OX=224308 GN=... [more]
Match NameE-valueIdentityDescription
A0A0A0K4222.4e-18799.11Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G064010 PE=4 SV=1[more]
A0A5A7SPI98.6e-18597.62Putative nitronate monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3C1I88.6e-18597.62probable nitronate monooxygenase OS=Cucumis melo OX=3656 GN=LOC103495406 PE=4 SV... [more]
A0A6J1GL383.7e-18094.05uncharacterized protein LOC111454946 OS=Cucurbita moschata OX=3662 GN=LOC1114549... [more]
A0A6J1I3E91.3e-17793.13uncharacterized protein LOC111470639 OS=Cucurbita maxima OX=3661 GN=LOC111470639... [more]
Match NameE-valueIdentityDescription
XP_031744639.11.24e-23899.11uncharacterized protein LOC101221123 [Cucumis sativus][more]
XP_008455173.12.80e-23597.62PREDICTED: probable nitronate monooxygenase [Cucumis melo] >KAA0031461.1 putativ... [more]
XP_022952200.13.50e-22994.05uncharacterized protein LOC111454946 [Cucurbita moschata] >KAG7011538.1 yrpB [Cu... [more]
XP_023511402.11.42e-22894.05uncharacterized protein LOC111776237 [Cucurbita pepo subsp. pepo][more]
XP_038888874.14.08e-22894.64nitronate monooxygenase isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT5G64250.26.8e-15078.19Aldolase-type TIM barrel family protein [more]
AT5G64250.12.4e-12676.43Aldolase-type TIM barrel family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004136Nitronate monooxygenasePFAMPF03060NMOcoord: 76..319
e-value: 6.3E-44
score: 150.7
IPR004136Nitronate monooxygenaseCDDcd04730NPD_likecoord: 10..234
e-value: 1.49807E-78
score: 238.152
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 3..335
e-value: 3.0E-97
score: 327.7
NoneNo IPR availablePANTHERPTHR32332:SF35SUBFAMILY NOT NAMEDcoord: 2..335
NoneNo IPR availablePANTHERPTHR323322-NITROPROPANE DIOXYGENASEcoord: 2..335
NoneNo IPR availableSUPERFAMILY51412Inosine monophosphate dehydrogenase (IMPDH)coord: 3..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy1G002340.1Chy1G002340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0018580 nitronate monooxygenase activity
molecular_function GO:0003824 catalytic activity