Chy12G219340 (gene) Cucumber (hystrix) v1

Overview
NameChy12G219340
Typegene
OrganismCucumis hystrix (Cucumber (hystrix) v1)
Descriptionglucuronoxylan 4-O-methyltransferase 1
LocationchrH12: 17680641 .. 17681576 (+)
RNA-Seq ExpressionChy12G219340
SyntenyChy12G219340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTTTAAATTTCCATATCCCAAATCTCTTTACTTCAACAACAAAAGAAAAATAGTAATAACAATGAGGCAAAAAACATACCCCATTCATCAATTTCTCAACCTCAAAACCCTCCTCTTAGTTCTATTTCTTTCACTTGTTCTCATCTTTCTCTTCAAATCTAACATTCTTAACTCACCCACACAAAACCCATCTCCAAAGTTACAATCTTTTTCACAAAAGTTAACTCATTGCTCCTCTCCATCTTTTTGCAACAAAATCCCTCCCTCATTTTCCCACACTCTCATCCATTACTCCACCTCCTCCATAACCCCACAACAAACATTCAAAGAAATCTCCGTCGCCGCTGCCGTCCTCCTCGACCGATCCCCATGCAACTTCCTTGTCTTCGGCCTCGGCCACGACAGCCTCATGTGGGCCACTCTCAACCACGGTGGTCGGACATTATTCCTTGAAGAAGACAAATCATGGATCCAACAAATAAGTCGTCGTTTTCCGATGCTTGAGTCTCGTCACGTTATATATGATAGCAAGGTCCATCAGGCCGACGGTTTGATGGATGTTGGGAAGGGGCCAGAGTGTACTGCAGTCGGAGACCCGAAATACTCAGTTTGCCCGTTGGCGATTAAAGGTTTGCCGGAGGAAGTGTATGCGGTGAAATGGGATTTGATTATGGTGGACGCGCCGACGGGGTTCCACGACGAGGCGCCGGGGAGGATGACGGCGATTTATACAGCAGGGATGATGGCGAGGAATAGAGAGGAAGGAGGAAGTACGGACGTGTTTGTGCATGATGTGAATAGAGAAGTGGAAGATAAGTTCTCAATGGAGTTTTTATGCGAAGGTTATATGGTTAAACAAGAAGGGAGACTGAGACATTTTACCATTCCTTCTCATAGGGATGATTTAGATAAACCTTTTTGCCCTTTCTAA

mRNA sequence

ATGGCCTTTAAATTTCCATATCCCAAATCTCTTTACTTCAACAACAAAAGAAAAATAGTAATAACAATGAGGCAAAAAACATACCCCATTCATCAATTTCTCAACCTCAAAACCCTCCTCTTAGTTCTATTTCTTTCACTTGTTCTCATCTTTCTCTTCAAATCTAACATTCTTAACTCACCCACACAAAACCCATCTCCAAAGTTACAATCTTTTTCACAAAAGTTAACTCATTGCTCCTCTCCATCTTTTTGCAACAAAATCCCTCCCTCATTTTCCCACACTCTCATCCATTACTCCACCTCCTCCATAACCCCACAACAAACATTCAAAGAAATCTCCGTCGCCGCTGCCGTCCTCCTCGACCGATCCCCATGCAACTTCCTTGTCTTCGGCCTCGGCCACGACAGCCTCATGTGGGCCACTCTCAACCACGGTGGTCGGACATTATTCCTTGAAGAAGACAAATCATGGATCCAACAAATAAGTCGTCGTTTTCCGATGCTTGAGTCTCGTCACGTTATATATGATAGCAAGGTCCATCAGGCCGACGGTTTGATGGATGTTGGGAAGGGGCCAGAGTGTACTGCAGTCGGAGACCCGAAATACTCAGTTTGCCCGTTGGCGATTAAAGGTTTGCCGGAGGAAGTGTATGCGGTGAAATGGGATTTGATTATGGTGGACGCGCCGACGGGGTTCCACGACGAGGCGCCGGGGAGGATGACGGCGATTTATACAGCAGGGATGATGGCGAGGAATAGAGAGGAAGGAGGAAGTACGGACGTGTTTGTGCATGATGTGAATAGAGAAGTGGAAGATAAGTTCTCAATGGAGTTTTTATGCGAAGGTTATATGGTTAAACAAGAAGGGAGACTGAGACATTTTACCATTCCTTCTCATAGGGATGATTTAGATAAACCTTTTTGCCCTTTCTAA

Coding sequence (CDS)

ATGGCCTTTAAATTTCCATATCCCAAATCTCTTTACTTCAACAACAAAAGAAAAATAGTAATAACAATGAGGCAAAAAACATACCCCATTCATCAATTTCTCAACCTCAAAACCCTCCTCTTAGTTCTATTTCTTTCACTTGTTCTCATCTTTCTCTTCAAATCTAACATTCTTAACTCACCCACACAAAACCCATCTCCAAAGTTACAATCTTTTTCACAAAAGTTAACTCATTGCTCCTCTCCATCTTTTTGCAACAAAATCCCTCCCTCATTTTCCCACACTCTCATCCATTACTCCACCTCCTCCATAACCCCACAACAAACATTCAAAGAAATCTCCGTCGCCGCTGCCGTCCTCCTCGACCGATCCCCATGCAACTTCCTTGTCTTCGGCCTCGGCCACGACAGCCTCATGTGGGCCACTCTCAACCACGGTGGTCGGACATTATTCCTTGAAGAAGACAAATCATGGATCCAACAAATAAGTCGTCGTTTTCCGATGCTTGAGTCTCGTCACGTTATATATGATAGCAAGGTCCATCAGGCCGACGGTTTGATGGATGTTGGGAAGGGGCCAGAGTGTACTGCAGTCGGAGACCCGAAATACTCAGTTTGCCCGTTGGCGATTAAAGGTTTGCCGGAGGAAGTGTATGCGGTGAAATGGGATTTGATTATGGTGGACGCGCCGACGGGGTTCCACGACGAGGCGCCGGGGAGGATGACGGCGATTTATACAGCAGGGATGATGGCGAGGAATAGAGAGGAAGGAGGAAGTACGGACGTGTTTGTGCATGATGTGAATAGAGAAGTGGAAGATAAGTTCTCAATGGAGTTTTTATGCGAAGGTTATATGGTTAAACAAGAAGGGAGACTGAGACATTTTACCATTCCTTCTCATAGGGATGATTTAGATAAACCTTTTTGCCCTTTCTAA

Protein sequence

MAFKFPYPKSLYFNNKRKIVITMRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSPSFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWATLNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPKYSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDVFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF*
Homology
BLAST of Chy12G219340 vs. ExPASy Swiss-Prot
Match: Q6NMK1 (Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 PE=1 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.4e-106
Identity = 191/292 (65.41%), Postives = 231/292 (79.11%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSP 82
           MR K    H+   LK LL+ L  +L+LIF+ +S + +S       + +S     T CS  
Sbjct: 1   MRPKANQNHK---LKVLLVFLLATLILIFIVRSTLTSSQEHQTPQETRS-----TRCSGA 60

Query: 83  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 142
             CNK+P S +  LIHYSTS ITPQQT KEI+V++ VL  +SPCNFLVFGLGHDSLMW++
Sbjct: 61  --CNKLPRSLAQALIHYSTSVITPQQTLKEIAVSSRVLGKKSPCNFLVFGLGHDSLMWSS 120

Query: 143 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 202
           LN+GGRT+FLEED++WI+QI RRFPMLES HV YDSKV+QAD L++VGKGPECTA+GDP+
Sbjct: 121 LNYGGRTVFLEEDEAWIKQIKRRFPMLESYHVTYDSKVNQADNLIEVGKGPECTAIGDPR 180

Query: 203 YSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 262
           YS+C LA+KGLP E+Y   WDLIMVDAPTG++DEAPGRMTAIYTAGMMARNR++GG TDV
Sbjct: 181 YSMCQLALKGLPAEIYETGWDLIMVDAPTGYYDEAPGRMTAIYTAGMMARNRKQGGETDV 240

Query: 263 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRD----DLDKPFCP 311
           FVHDVNRE+EDKFS  FLCEGYM KQEGRLRHF IPS+RD    + ++PFCP
Sbjct: 241 FVHDVNREIEDKFSKAFLCEGYMKKQEGRLRHFIIPSYRDGSESESNRPFCP 282

BLAST of Chy12G219340 vs. ExPASy Swiss-Prot
Match: Q9LQ32 (Glucuronoxylan 4-O-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GXM3 PE=1 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 7.6e-92
Identity = 165/283 (58.30%), Postives = 212/283 (74.91%), Query Frame = 0

Query: 34  LNLKTLLL-VLFLSLVLIFLFKSNILNSPTQ-----NPSPKLQSFSQKLTHCSSPSFCNK 93
           LNLK + +    L L++I+L +SNI +S ++     N S + +    K   C +   C K
Sbjct: 9   LNLKVIFIGSSILILIIIYLARSNISSSSSKPISKTNLSQEEEETQHKQEGCPTTQQCTK 68

Query: 94  IPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWATLNHGG 153
           +P S S  L+HY TS++TPQQTF E+SV+  VL  +SPCNFLVFGLGHDSLMWA+LNHGG
Sbjct: 69  MPLSLSDALVHYVTSNVTPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLNHGG 128

Query: 154 RTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPKYSVCP 213
           RTLF+EED++WI  ++++FP LES HV+YD+KV  +D LM++G+  EC +V DP+ S C 
Sbjct: 129 RTLFIEEDQAWIAIVTKKFPNLESYHVVYDTKVKDSDKLMELGRSEECRSVSDPRNSKCD 188

Query: 214 LAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDVFVHDV 273
           LA+K  P + Y  KWDLIMVDAPTG+H+EAPGRM+AIYTAG++ARNRE+ G TDVFVHDV
Sbjct: 189 LALKDFPADFYETKWDLIMVDAPTGYHEEAPGRMSAIYTAGLLARNRED-GETDVFVHDV 248

Query: 274 NREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCP 311
           NR VED+FS  FLC+GYM +Q GRLRHFTIPSHR    +PFCP
Sbjct: 249 NRPVEDEFSATFLCKGYMREQNGRLRHFTIPSHRARAGRPFCP 290

BLAST of Chy12G219340 vs. ExPASy Swiss-Prot
Match: Q9T0F7 (Glucuronoxylan 4-O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GXM2 PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 1.9e-90
Identity = 154/275 (56.00%), Postives = 206/275 (74.91%), Query Frame = 0

Query: 36  LKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSPSFCNKIPPSFSHT 95
           + + L+ +  S++++F+      +  + + +     + QK    S+P  C K+P S S  
Sbjct: 9   ISSKLIFICCSILVLFILFLKRASFSSNSTATIRDEYHQKSKCPSTPQQCTKLPTSLSDA 68

Query: 96  LIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWATLNHGGRTLFLEED 155
           L+HY TS ITPQQTF E+SV+  VL  +SPCNFLVFGLGHDSLMWA+LNHGGRTLFLEED
Sbjct: 69  LVHYVTSEITPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLNHGGRTLFLEED 128

Query: 156 KSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPKYSVCPLAIKGLPE 215
           ++WI+ ++++FP LES HV+YD+KV  ++ LM++ +  +C AV DP+ S C L++KG P 
Sbjct: 129 EAWIETVTKKFPNLESYHVVYDTKVKDSNKLMELKRTEDCKAVSDPRDSKCALSLKGFPA 188

Query: 216 EVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDVFVHDVNREVEDKF 275
           +VY  +WD+IMVDAPTG+HDEAPGRM+AIYTAG++ARNR +GG TDVFVHD+NR VED+F
Sbjct: 189 DVYETQWDVIMVDAPTGYHDEAPGRMSAIYTAGLLARNRYDGGETDVFVHDINRPVEDEF 248

Query: 276 SMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCP 311
           S+ FLC GYM +Q+GRLRHF IPSHR     PFCP
Sbjct: 249 SVAFLCGGYMKEQQGRLRHFNIPSHRASFGTPFCP 283

BLAST of Chy12G219340 vs. ExPASy Swiss-Prot
Match: Q9C7F9 (Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g27930 PE=1 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 8.8e-56
Identity = 108/209 (51.67%), Postives = 142/209 (67.94%), Query Frame = 0

Query: 96  LIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWATLNHGGRTLFLEED 155
           ++HY+TS++ PQQ   EIS++  +L   +P NFLVFGLG DSLMWA+LN  G+TLFLEED
Sbjct: 68  IVHYATSNVVPQQNLAEISISFNILKKLAPANFLVFGLGRDSLMWASLNPRGKTLFLEED 127

Query: 156 KSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGK-GPECTAV-----GDPKYSVCPLA 215
             W Q++++  P L + HV Y +++ QAD L+   K  P+C        G+ K   C LA
Sbjct: 128 LEWFQKVTKDSPFLRAHHVRYRTQLQQADSLLRSYKTEPKCFPAKSYLRGNEK---CKLA 187

Query: 216 IKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDVFVHDVNR 275
           + GLP+E Y  +WDL+MVDAP G+  EAPGRM AI++A +MARNR++ G T VF+HDVNR
Sbjct: 188 LTGLPDEFYDTEWDLLMVDAPKGYFAEAPGRMAAIFSAAVMARNRKKPGVTHVFLHDVNR 247

Query: 276 EVEDKFSMEFLCEGYMVKQEGRLRHFTIP 299
            VE  F+ EFLC  Y V   GRL HF IP
Sbjct: 248 RVEKTFAEEFLCRKYRVNAAGRLWHFAIP 273

BLAST of Chy12G219340 vs. ExPASy Swiss-Prot
Match: Q9SNE5 (Protein IRREGULAR XYLEM 15 OS=Arabidopsis thaliana OX=3702 GN=IRX15 PE=2 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.1e-37
Identity = 89/233 (38.20%), Postives = 133/233 (57.08%), Query Frame = 0

Query: 80  SSPSFCNKIPPSFSHTLIHY-STSSITPQQTFKEI-SVAAAVLLDRSPCNFLVFGLGHDS 139
           SSP   + +P S  + L+HY S S+ +   ++ E+ S++  +     PCN LVFGL H++
Sbjct: 76  SSPISNSNLPTSAINALLHYASRSNDSFHMSYGEMKSISDVLRRCAPPCNLLVFGLTHET 135

Query: 140 LMWATLNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGK---GPE 199
           L+W +LNH GRT+F+EE++ +        P ++   V Y +K H+A  L+   K   G E
Sbjct: 136 LLWKSLNHNGRTVFIEENRYYAAYFEEIHPEIDVFDVQYTTKAHEAGELVTAAKEAAGNE 195

Query: 200 CTAVGDPKYSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNR 259
           C  V +  +S C L +  LP  VY V WD+I VD P G   E PGRM++I+TA ++AR++
Sbjct: 196 CRPVQNLLFSDCKLGLNDLPNHVYDVDWDVIFVDGPRGDAHEGPGRMSSIFTAAVLARSK 255

Query: 260 EEG-GSTDVFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDK 307
           + G   T VFVHD  R+VE     EFLC   +V+    L H+ +    D +DK
Sbjct: 256 KGGTPKTHVFVHDYYRDVERLCGDEFLCRENLVESNDLLAHYVL----DKMDK 304

BLAST of Chy12G219340 vs. ExPASy TrEMBL
Match: A0A0A0LTA4 (Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G044930 PE=4 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 2.9e-163
Identity = 283/289 (97.92%), Postives = 286/289 (98.96%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSP 82
           MRQKTYPIHQFLNLKTLLLVLFLS VLIFLFKSNIL+SPTQNPSPKLQSFSQKLTHCSSP
Sbjct: 1   MRQKTYPIHQFLNLKTLLLVLFLSFVLIFLFKSNILDSPTQNPSPKLQSFSQKLTHCSSP 60

Query: 83  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 142
           SFCNKIPPSFSH LIHYSTSSITPQQTFKEISVAAAVLL+RSPCNFLVFGLGHDSLMWAT
Sbjct: 61  SFCNKIPPSFSHALIHYSTSSITPQQTFKEISVAAAVLLNRSPCNFLVFGLGHDSLMWAT 120

Query: 143 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 202
           LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK
Sbjct: 121 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 180

Query: 203 YSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 262
           YSVCPLAIKGLPEEVY VKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV
Sbjct: 181 YSVCPLAIKGLPEEVYEVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 240

Query: 263 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 312
           FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSH+DDLDKPFCPF
Sbjct: 241 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHKDDLDKPFCPF 289

BLAST of Chy12G219340 vs. ExPASy TrEMBL
Match: A0A1S3ATM9 (glucuronoxylan 4-O-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103482841 PE=4 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 8.0e-161
Identity = 282/289 (97.58%), Postives = 283/289 (97.92%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSP 82
           MRQKT PIHQFLNLKTLLLVLFLS  LIFLFKSNI +SPTQNPSPKLQSFSQKLTHC SP
Sbjct: 1   MRQKTNPIHQFLNLKTLLLVLFLSFFLIFLFKSNIFDSPTQNPSPKLQSFSQKLTHC-SP 60

Query: 83  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 142
           SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT
Sbjct: 61  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 120

Query: 143 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 202
           LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK
Sbjct: 121 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 180

Query: 203 YSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 262
           YSVCPLAIKGLPEEVY VKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV
Sbjct: 181 YSVCPLAIKGLPEEVYEVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 240

Query: 263 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 312
           FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF
Sbjct: 241 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 288

BLAST of Chy12G219340 vs. ExPASy TrEMBL
Match: A0A6J1GSM4 (glucuronoxylan 4-O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111457072 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 5.4e-133
Identity = 240/290 (82.76%), Postives = 254/290 (87.59%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFS-QKLTHCSS 82
           MR KT+P+HQFLNLKTL+L LFLSL  IF  KS I +S TQNPSPK QSFS Q   +C  
Sbjct: 1   MRPKTHPMHQFLNLKTLILALFLSLFFIFFMKSTIFDSSTQNPSPKFQSFSPQAPKNC-- 60

Query: 83  PSFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWA 142
            + CNKIP S SH LIHY+TSSITPQQT KEI+VAA+VL  RSPCNFLVFGLGHDSLMWA
Sbjct: 61  -THCNKIPRSLSHALIHYATSSITPQQTLKEITVAASVLAVRSPCNFLVFGLGHDSLMWA 120

Query: 143 TLNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDP 202
           TLNHGGRTLFLEED+SWIQQI RRFPMLESRH  YDSKV QAD LMDVGKG EC AVGDP
Sbjct: 121 TLNHGGRTLFLEEDESWIQQIHRRFPMLESRHACYDSKVQQADSLMDVGKGLECRAVGDP 180

Query: 203 KYSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTD 262
           KYSVC LA KGLPEEVY VKWDLIMVDAPTG++D+APGRMTAIYTAGMMARNREEGG TD
Sbjct: 181 KYSVCQLAQKGLPEEVYDVKWDLIMVDAPTGYYDDAPGRMTAIYTAGMMARNREEGGITD 240

Query: 263 VFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 312
           VFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHF IPSHRD+LDKPFCPF
Sbjct: 241 VFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFRIPSHRDELDKPFCPF 287

BLAST of Chy12G219340 vs. ExPASy TrEMBL
Match: A0A6J1JWG6 (glucuronoxylan 4-O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111490300 PE=4 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 2.1e-132
Identity = 238/289 (82.35%), Postives = 252/289 (87.20%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSP 82
           MR KT+P+HQFLNLKTL+L LFLSL  IFL KS I +S TQNPS K QSFS +     +P
Sbjct: 1   MRPKTHPMHQFLNLKTLILALFLSLFFIFLMKSTIFDSSTQNPSQKFQSFSSQAPKNCTP 60

Query: 83  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 142
             CNKIP S SH LIHY+TSSITPQQT KEI VAA+VL  RSPCNFLVFGLGHDSLMWAT
Sbjct: 61  --CNKIPRSLSHALIHYATSSITPQQTLKEIMVAASVLAVRSPCNFLVFGLGHDSLMWAT 120

Query: 143 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 202
           LNHGGRTLFLEED+SWIQQI RRFPMLESRH  YDSKV QAD LMDVGKG EC AVGDPK
Sbjct: 121 LNHGGRTLFLEEDESWIQQIHRRFPMLESRHACYDSKVQQADSLMDVGKGLECRAVGDPK 180

Query: 203 YSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 262
           YSVC LA KGLPEEVY +KWDLIMVDAPTG++D+APGRMTAIYTAGMMARNREEGG TDV
Sbjct: 181 YSVCQLAQKGLPEEVYDMKWDLIMVDAPTGYYDDAPGRMTAIYTAGMMARNREEGGITDV 240

Query: 263 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 312
           FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHF IPSHRD+LDKPFCPF
Sbjct: 241 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFRIPSHRDELDKPFCPF 287

BLAST of Chy12G219340 vs. ExPASy TrEMBL
Match: A0A6J1C6R8 (glucuronoxylan 4-O-methyltransferase 1-like OS=Momordica charantia OX=3673 GN=LOC111008917 PE=4 SV=1)

HSP 1 Score: 459.5 bits (1181), Expect = 1.1e-125
Identity = 229/295 (77.63%), Postives = 248/295 (84.07%), Query Frame = 0

Query: 23  MRQKTY-PIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSS 82
           MR KT+ PIHQ  NLKTLLL LFL+  LIFLFKS+       NPSPK QSFS K  + S+
Sbjct: 1   MRPKTHPPIHQLFNLKTLLLALFLAFPLIFLFKSHFF----ANPSPKFQSFSPKTLNYST 60

Query: 83  -----PSFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHD 142
                P+ CNKIPPS +H L+HYSTSSI PQQT KEISV A VL +RSPCNFLVFGLGHD
Sbjct: 61  TRNCPPNSCNKIPPSLAHALVHYSTSSIIPQQTLKEISVTAGVLDERSPCNFLVFGLGHD 120

Query: 143 SLMWATLNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECT 202
           SLMWATLNHGGRT+FLEED SWIQQI RRFPMLES HV YD+KV+QAD LMDVGKGPECT
Sbjct: 121 SLMWATLNHGGRTVFLEEDDSWIQQIRRRFPMLESYHVTYDTKVNQADILMDVGKGPECT 180

Query: 203 AVGDPKYSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREE 262
           AVGDPKYS+C LA+KGLPEEVY VKWDLIMVDAPTG++D+APGRM AIYTAGMMARNREE
Sbjct: 181 AVGDPKYSMCQLALKGLPEEVYEVKWDLIMVDAPTGYYDDAPGRMAAIYTAGMMARNREE 240

Query: 263 GGSTDVFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 312
           GG TDVFVHDVNREVED+FS EFLC GYM  QEGRLRHF IPSHRDD+D PFCPF
Sbjct: 241 GGITDVFVHDVNREVEDRFSKEFLCRGYMKNQEGRLRHFRIPSHRDDMDIPFCPF 291

BLAST of Chy12G219340 vs. NCBI nr
Match: XP_004152531.1 (glucuronoxylan 4-O-methyltransferase 1 [Cucumis sativus] >KGN64244.1 hypothetical protein Csa_013930 [Cucumis sativus])

HSP 1 Score: 580 bits (1496), Expect = 1.39e-208
Identity = 283/289 (97.92%), Postives = 286/289 (98.96%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSP 82
           MRQKTYPIHQFLNLKTLLLVLFLS VLIFLFKSNIL+SPTQNPSPKLQSFSQKLTHCSSP
Sbjct: 1   MRQKTYPIHQFLNLKTLLLVLFLSFVLIFLFKSNILDSPTQNPSPKLQSFSQKLTHCSSP 60

Query: 83  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 142
           SFCNKIPPSFSH LIHYSTSSITPQQTFKEISVAAAVLL+RSPCNFLVFGLGHDSLMWAT
Sbjct: 61  SFCNKIPPSFSHALIHYSTSSITPQQTFKEISVAAAVLLNRSPCNFLVFGLGHDSLMWAT 120

Query: 143 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 202
           LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK
Sbjct: 121 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 180

Query: 203 YSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 262
           YSVCPLAIKGLPEEVY VKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV
Sbjct: 181 YSVCPLAIKGLPEEVYEVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 240

Query: 263 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 311
           FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSH+DDLDKPFCPF
Sbjct: 241 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHKDDLDKPFCPF 289

BLAST of Chy12G219340 vs. NCBI nr
Match: XP_008437427.1 (PREDICTED: glucuronoxylan 4-O-methyltransferase 1 [Cucumis melo])

HSP 1 Score: 573 bits (1476), Expect = 1.50e-205
Identity = 282/289 (97.58%), Postives = 283/289 (97.92%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSP 82
           MRQKT PIHQFLNLKTLLLVLFLS  LIFLFKSNI +SPTQNPSPKLQSFSQKLTHCS P
Sbjct: 1   MRQKTNPIHQFLNLKTLLLVLFLSFFLIFLFKSNIFDSPTQNPSPKLQSFSQKLTHCS-P 60

Query: 83  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 142
           SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT
Sbjct: 61  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 120

Query: 143 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 202
           LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK
Sbjct: 121 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 180

Query: 203 YSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 262
           YSVCPLAIKGLPEEVY VKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV
Sbjct: 181 YSVCPLAIKGLPEEVYEVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 240

Query: 263 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 311
           FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF
Sbjct: 241 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 288

BLAST of Chy12G219340 vs. NCBI nr
Match: XP_038895348.1 (glucuronoxylan 4-O-methyltransferase 1 [Benincasa hispida])

HSP 1 Score: 524 bits (1349), Expect = 3.65e-186
Identity = 259/291 (89.00%), Postives = 271/291 (93.13%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLT--HCS 82
           MRQKT+PIHQFLNLKTLLL LFLS  LIFL KSNI +SPTQNPSPKLQSFSQK T  HCS
Sbjct: 1   MRQKTHPIHQFLNLKTLLLALFLSFFLIFLLKSNIFHSPTQNPSPKLQSFSQKTTKNHCS 60

Query: 83  SPSFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMW 142
            PS CNKIPPSFSHTLIHYSTSSITPQQTFKEISV AAVLL+RSPCNFLVFGLGHDSLMW
Sbjct: 61  -PSACNKIPPSFSHTLIHYSTSSITPQQTFKEISVTAAVLLNRSPCNFLVFGLGHDSLMW 120

Query: 143 ATLNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGD 202
           ATLNHGGRTLFLEED+SWI+QI  RFPMLES HV Y+SKV QAD LMDVGKGPEC AVGD
Sbjct: 121 ATLNHGGRTLFLEEDESWIEQIRHRFPMLESHHVRYNSKVDQADSLMDVGKGPECMAVGD 180

Query: 203 PKYSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGST 262
           PKYSVC LA+KGLPEEVY VKWDLIMVDAPTG+++EAPGRMTAIYTAGMMARNREEGGST
Sbjct: 181 PKYSVCQLALKGLPEEVYEVKWDLIMVDAPTGYYEEAPGRMTAIYTAGMMARNREEGGST 240

Query: 263 DVFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 311
           D+FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDL+KPFCPF
Sbjct: 241 DLFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLNKPFCPF 290

BLAST of Chy12G219340 vs. NCBI nr
Match: XP_022954973.1 (glucuronoxylan 4-O-methyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 480 bits (1236), Expect = 5.33e-169
Identity = 240/290 (82.76%), Postives = 254/290 (87.59%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFS-QKLTHCSS 82
           MR KT+P+HQFLNLKTL+L LFLSL  IF  KS I +S TQNPSPK QSFS Q   +C+ 
Sbjct: 1   MRPKTHPMHQFLNLKTLILALFLSLFFIFFMKSTIFDSSTQNPSPKFQSFSPQAPKNCT- 60

Query: 83  PSFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWA 142
              CNKIP S SH LIHY+TSSITPQQT KEI+VAA+VL  RSPCNFLVFGLGHDSLMWA
Sbjct: 61  --HCNKIPRSLSHALIHYATSSITPQQTLKEITVAASVLAVRSPCNFLVFGLGHDSLMWA 120

Query: 143 TLNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDP 202
           TLNHGGRTLFLEED+SWIQQI RRFPMLESRH  YDSKV QAD LMDVGKG EC AVGDP
Sbjct: 121 TLNHGGRTLFLEEDESWIQQIHRRFPMLESRHACYDSKVQQADSLMDVGKGLECRAVGDP 180

Query: 203 KYSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTD 262
           KYSVC LA KGLPEEVY VKWDLIMVDAPTG++D+APGRMTAIYTAGMMARNREEGG TD
Sbjct: 181 KYSVCQLAQKGLPEEVYDVKWDLIMVDAPTGYYDDAPGRMTAIYTAGMMARNREEGGITD 240

Query: 263 VFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 311
           VFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHF IPSHRD+LDKPFCPF
Sbjct: 241 VFVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFRIPSHRDELDKPFCPF 287

BLAST of Chy12G219340 vs. NCBI nr
Match: XP_022994652.1 (glucuronoxylan 4-O-methyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 478 bits (1231), Expect = 3.08e-168
Identity = 238/289 (82.35%), Postives = 252/289 (87.20%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSP 82
           MR KT+P+HQFLNLKTL+L LFLSL  IFL KS I +S TQNPS K QSFS +     +P
Sbjct: 1   MRPKTHPMHQFLNLKTLILALFLSLFFIFLMKSTIFDSSTQNPSQKFQSFSSQAPKNCTP 60

Query: 83  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 142
             CNKIP S SH LIHY+TSSITPQQT KEI VAA+VL  RSPCNFLVFGLGHDSLMWAT
Sbjct: 61  --CNKIPRSLSHALIHYATSSITPQQTLKEIMVAASVLAVRSPCNFLVFGLGHDSLMWAT 120

Query: 143 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 202
           LNHGGRTLFLEED+SWIQQI RRFPMLESRH  YDSKV QAD LMDVGKG EC AVGDPK
Sbjct: 121 LNHGGRTLFLEEDESWIQQIHRRFPMLESRHACYDSKVQQADSLMDVGKGLECRAVGDPK 180

Query: 203 YSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 262
           YSVC LA KGLPEEVY +KWDLIMVDAPTG++D+APGRMTAIYTAGMMARNREEGG TDV
Sbjct: 181 YSVCQLAQKGLPEEVYDMKWDLIMVDAPTGYYDDAPGRMTAIYTAGMMARNREEGGITDV 240

Query: 263 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCPF 311
           FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHF IPSHRD+LDKPFCPF
Sbjct: 241 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFRIPSHRDELDKPFCPF 287

BLAST of Chy12G219340 vs. TAIR 10
Match: AT1G09610.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 387.5 bits (994), Expect = 1.0e-107
Identity = 191/292 (65.41%), Postives = 231/292 (79.11%), Query Frame = 0

Query: 23  MRQKTYPIHQFLNLKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSP 82
           MR K    H+   LK LL+ L  +L+LIF+ +S + +S       + +S     T CS  
Sbjct: 1   MRPKANQNHK---LKVLLVFLLATLILIFIVRSTLTSSQEHQTPQETRS-----TRCSGA 60

Query: 83  SFCNKIPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWAT 142
             CNK+P S +  LIHYSTS ITPQQT KEI+V++ VL  +SPCNFLVFGLGHDSLMW++
Sbjct: 61  --CNKLPRSLAQALIHYSTSVITPQQTLKEIAVSSRVLGKKSPCNFLVFGLGHDSLMWSS 120

Query: 143 LNHGGRTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPK 202
           LN+GGRT+FLEED++WI+QI RRFPMLES HV YDSKV+QAD L++VGKGPECTA+GDP+
Sbjct: 121 LNYGGRTVFLEEDEAWIKQIKRRFPMLESYHVTYDSKVNQADNLIEVGKGPECTAIGDPR 180

Query: 203 YSVCPLAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDV 262
           YS+C LA+KGLP E+Y   WDLIMVDAPTG++DEAPGRMTAIYTAGMMARNR++GG TDV
Sbjct: 181 YSMCQLALKGLPAEIYETGWDLIMVDAPTGYYDEAPGRMTAIYTAGMMARNRKQGGETDV 240

Query: 263 FVHDVNREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRD----DLDKPFCP 311
           FVHDVNRE+EDKFS  FLCEGYM KQEGRLRHF IPS+RD    + ++PFCP
Sbjct: 241 FVHDVNREIEDKFSKAFLCEGYMKKQEGRLRHFIIPSYRDGSESESNRPFCP 282

BLAST of Chy12G219340 vs. TAIR 10
Match: AT1G33800.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 338.6 bits (867), Expect = 5.4e-93
Identity = 165/283 (58.30%), Postives = 212/283 (74.91%), Query Frame = 0

Query: 34  LNLKTLLL-VLFLSLVLIFLFKSNILNSPTQ-----NPSPKLQSFSQKLTHCSSPSFCNK 93
           LNLK + +    L L++I+L +SNI +S ++     N S + +    K   C +   C K
Sbjct: 9   LNLKVIFIGSSILILIIIYLARSNISSSSSKPISKTNLSQEEEETQHKQEGCPTTQQCTK 68

Query: 94  IPPSFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWATLNHGG 153
           +P S S  L+HY TS++TPQQTF E+SV+  VL  +SPCNFLVFGLGHDSLMWA+LNHGG
Sbjct: 69  MPLSLSDALVHYVTSNVTPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLNHGG 128

Query: 154 RTLFLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPKYSVCP 213
           RTLF+EED++WI  ++++FP LES HV+YD+KV  +D LM++G+  EC +V DP+ S C 
Sbjct: 129 RTLFIEEDQAWIAIVTKKFPNLESYHVVYDTKVKDSDKLMELGRSEECRSVSDPRNSKCD 188

Query: 214 LAIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDVFVHDV 273
           LA+K  P + Y  KWDLIMVDAPTG+H+EAPGRM+AIYTAG++ARNRE+ G TDVFVHDV
Sbjct: 189 LALKDFPADFYETKWDLIMVDAPTGYHEEAPGRMSAIYTAGLLARNRED-GETDVFVHDV 248

Query: 274 NREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCP 311
           NR VED+FS  FLC+GYM +Q GRLRHFTIPSHR    +PFCP
Sbjct: 249 NRPVEDEFSATFLCKGYMREQNGRLRHFTIPSHRARAGRPFCP 290

BLAST of Chy12G219340 vs. TAIR 10
Match: AT4G09990.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 334.0 bits (855), Expect = 1.3e-91
Identity = 154/275 (56.00%), Postives = 206/275 (74.91%), Query Frame = 0

Query: 36  LKTLLLVLFLSLVLIFLFKSNILNSPTQNPSPKLQSFSQKLTHCSSPSFCNKIPPSFSHT 95
           + + L+ +  S++++F+      +  + + +     + QK    S+P  C K+P S S  
Sbjct: 9   ISSKLIFICCSILVLFILFLKRASFSSNSTATIRDEYHQKSKCPSTPQQCTKLPTSLSDA 68

Query: 96  LIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWATLNHGGRTLFLEED 155
           L+HY TS ITPQQTF E+SV+  VL  +SPCNFLVFGLGHDSLMWA+LNHGGRTLFLEED
Sbjct: 69  LVHYVTSEITPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLNHGGRTLFLEED 128

Query: 156 KSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKGPECTAVGDPKYSVCPLAIKGLPE 215
           ++WI+ ++++FP LES HV+YD+KV  ++ LM++ +  +C AV DP+ S C L++KG P 
Sbjct: 129 EAWIETVTKKFPNLESYHVVYDTKVKDSNKLMELKRTEDCKAVSDPRDSKCALSLKGFPA 188

Query: 216 EVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDVFVHDVNREVEDKF 275
           +VY  +WD+IMVDAPTG+HDEAPGRM+AIYTAG++ARNR +GG TDVFVHD+NR VED+F
Sbjct: 189 DVYETQWDVIMVDAPTGYHDEAPGRMSAIYTAGLLARNRYDGGETDVFVHDINRPVEDEF 248

Query: 276 SMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKPFCP 311
           S+ FLC GYM +Q+GRLRHF IPSHR     PFCP
Sbjct: 249 SVAFLCGGYMKEQQGRLRHFNIPSHRASFGTPFCP 283

BLAST of Chy12G219340 vs. TAIR 10
Match: AT1G71690.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 310.1 bits (793), Expect = 2.1e-84
Identity = 164/275 (59.64%), Postives = 198/275 (72.00%), Query Frame = 0

Query: 39  LLLVLFLSLVLIFLF--------KSNILNSPTQNPSPKLQSFSQKLTHCSSPSFCNKIPP 98
           L+L  F+SLV++ LF         S+  + P  N      S S   +   SP  CNKIPP
Sbjct: 13  LILSSFVSLVVLTLFFITRTGFSPSSSFHQPLNN--TLRISTSSTGSKLQSPRSCNKIPP 72

Query: 99  SFSHTLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWATLNHGGRTL 158
           S +  L+HY++S++TPQQT  EISV    L  +SPCNFLVFGLGHDSLMWATLNHGGRT+
Sbjct: 73  SLADALVHYASSNVTPQQTLSEISVTKKELEKKSPCNFLVFGLGHDSLMWATLNHGGRTI 132

Query: 159 FLEEDKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKG-PECTAVG-DPKYSVCPL 218
           FL+ED+SWI QI+ +FP LES HV Y +KV  A+ LM   K   EC  V  D + S C L
Sbjct: 133 FLDEDESWIHQIAEKFPSLESYHVRYKTKVRDAEALMAATKDREECRRVSTDLRVSTCEL 192

Query: 219 AIKGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNR-EEGGSTDVFVHDV 278
           A+KGLPE VY  +WDLIMVDAPTGFH+EAPGRM+AIYTAGM+AR R +E  +T VFVHDV
Sbjct: 193 ALKGLPEVVYETEWDLIMVDAPTGFHEEAPGRMSAIYTAGMIARRRKDEEETTAVFVHDV 252

Query: 279 NREVEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRD 303
           +R+VED+FSMEFLC  YM KQEGRLRHFT+PSHR+
Sbjct: 253 DRKVEDEFSMEFLCRDYMTKQEGRLRHFTVPSHRN 285

BLAST of Chy12G219340 vs. TAIR 10
Match: AT1G67330.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 230.7 bits (587), Expect = 1.6e-60
Identity = 116/222 (52.25%), Postives = 154/222 (69.37%), Query Frame = 0

Query: 95  TLIHYSTSSITPQQTFKEISVAAAVLLDRSPCNFLVFGLGHDSLMWATLNHGGRTLFLEE 154
           +++HY+TS   PQQ+F+EIS++  VL +R PCNFLVFGLG DSLMWA+LN GG T+FLEE
Sbjct: 70  SIVHYATSHTVPQQSFEEISISLNVLKERLPCNFLVFGLGRDSLMWASLNPGGTTVFLEE 129

Query: 155 DKSWIQQISRRFPMLESRHVIYDSKVHQADGLMDVGKG-PEC-TAVGDP-KYS-VCPLAI 214
           D  WI+ + +  P L + HV Y + + +A  L+   K  P C  A   P +Y+  CPLA+
Sbjct: 130 DPEWIEAVLKDAPSLRAHHVQYRTHLSEAGRLLSTYKNEPMCLPAKAFPIRYNEKCPLAL 189

Query: 215 KGLPEEVYAVKWDLIMVDAPTGFHDEAPGRMTAIYTAGMMARNREEGGSTDVFVHDVNRE 274
             LP+E Y  +WDLIMVDAP G+  EAPGRM AI+++ +MARNR+  G+T VF+HDVNR+
Sbjct: 190 TSLPDEFYDTEWDLIMVDAPKGYFPEAPGRMAAIFSSAIMARNRKGDGTTHVFLHDVNRK 249

Query: 275 VEDKFSMEFLCEGYMVKQEGRLRHFTIPSHRDDLDKP---FC 310
           VE+ F+ EFLCE Y V   GRL HF IP+  +  D+P   FC
Sbjct: 250 VENAFANEFLCEKYKVNSVGRLWHFEIPNAANMTDQPGDRFC 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NMK11.4e-10665.41Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 P... [more]
Q9LQ327.6e-9258.30Glucuronoxylan 4-O-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GXM3 P... [more]
Q9T0F71.9e-9056.00Glucuronoxylan 4-O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GXM2 P... [more]
Q9C7F98.8e-5651.67Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g2793... [more]
Q9SNE53.1e-3738.20Protein IRREGULAR XYLEM 15 OS=Arabidopsis thaliana OX=3702 GN=IRX15 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0LTA42.9e-16397.92Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G04... [more]
A0A1S3ATM98.0e-16197.58glucuronoxylan 4-O-methyltransferase 1 OS=Cucumis melo OX=3656 GN=LOC103482841 P... [more]
A0A6J1GSM45.4e-13382.76glucuronoxylan 4-O-methyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC... [more]
A0A6J1JWG62.1e-13282.35glucuronoxylan 4-O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A6J1C6R81.1e-12577.63glucuronoxylan 4-O-methyltransferase 1-like OS=Momordica charantia OX=3673 GN=LO... [more]
Match NameE-valueIdentityDescription
XP_004152531.11.39e-20897.92glucuronoxylan 4-O-methyltransferase 1 [Cucumis sativus] >KGN64244.1 hypothetica... [more]
XP_008437427.11.50e-20597.58PREDICTED: glucuronoxylan 4-O-methyltransferase 1 [Cucumis melo][more]
XP_038895348.13.65e-18689.00glucuronoxylan 4-O-methyltransferase 1 [Benincasa hispida][more]
XP_022954973.15.33e-16982.76glucuronoxylan 4-O-methyltransferase 1-like [Cucurbita moschata][more]
XP_022994652.13.08e-16882.35glucuronoxylan 4-O-methyltransferase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT1G09610.11.0e-10765.41Protein of unknown function (DUF579) [more]
AT1G33800.15.4e-9358.30Protein of unknown function (DUF579) [more]
AT4G09990.11.3e-9156.00Protein of unknown function (DUF579) [more]
AT1G71690.12.1e-8459.64Protein of unknown function (DUF579) [more]
AT1G67330.11.6e-6052.25Protein of unknown function (DUF579) [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021148Polysaccharide biosynthesis domainPFAMPF04669Polysacc_synt_4coord: 111..295
e-value: 6.4E-70
score: 234.6
IPR006514IRX15/IRX15L/IGXMTIGRFAMTIGR01627TIGR01627coord: 87..309
e-value: 1.4E-87
score: 291.1
IPR006514IRX15/IRX15L/IGXMPANTHERPTHR31444OS11G0490100 PROTEINcoord: 31..310
NoneNo IPR availablePANTHERPTHR31444:SF26GLUCURONOXYLAN 4-O-METHYLTRANSFERASE 2-RELATEDcoord: 31..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Chy12G219340.1Chy12G219340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0009834 plant-type secondary cell wall biogenesis
biological_process GO:0045492 xylan biosynthetic process
cellular_component GO:0000139 Golgi membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030775 glucuronoxylan 4-O-methyltransferase activity