Chy12G214410 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G214410
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	berberine bridge enzyme-like 18
Location	chrH12: 13672909 .. 13673286 (-)
RNA-Seq Expression	Chy12G214410
Synteny	Chy12G214410

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGAAATTTCTGAATCAGAAACCCCCTCCCCACATCGAGCTGGATATCCCATACATATTGGCTACTACCTTACATGGCAACGACCCGACGCCGATTCAAGACACTTGAAGTGGGCTCGCGAGCTTCACAATTACATGACTCCTTTTGTGTCGAAATCGCCTAGAGCAGCATATGTAAATTACAGAGATCTTGACATGGGAACAAACAACGACGATGGGGTCCCGACCAGATGTGAGGAAGCAAGCATTTGGGGGCATCGATACTTTGGGAATAATTTTGAAAGGTTGATGGAAGTGAAAAGGAAGGTGGATCCATTTAACTTCTTCCGGCATGAGCAAAGCATACCACCAGCACCACCAAGAGTAGGAATCTAA

mRNA sequence

ATGAATGAAATTTCTGAATCAGAAACCCCCTCCCCACATCGAGCTGGATATCCCATACATATTGGCTACTACCTTACATGGCAACGACCCGACGCCGATTCAAGACACTTGAAGTGGGCTCGCGAGCTTCACAATTACATGACTCCTTTTGTGTCGAAATCGCCTAGAGCAGCATATGTAAATTACAGAGATCTTGACATGGGAACAAACAACGACGATGGGGTCCCGACCAGATGTGAGGAAGCAAGCATTTGGGGGCATCGATACTTTGGGAATAATTTTGAAAGGTTGATGGAAGTGAAAAGGAAGGTGGATCCATTTAACTTCTTCCGGCATGAGCAAAGCATACCACCAGCACCACCAAGAGTAGGAATCTAA

Coding sequence (CDS)

ATGAATGAAATTTCTGAATCAGAAACCCCCTCCCCACATCGAGCTGGATATCCCATACATATTGGCTACTACCTTACATGGCAACGACCCGACGCCGATTCAAGACACTTGAAGTGGGCTCGCGAGCTTCACAATTACATGACTCCTTTTGTGTCGAAATCGCCTAGAGCAGCATATGTAAATTACAGAGATCTTGACATGGGAACAAACAACGACGATGGGGTCCCGACCAGATGTGAGGAAGCAAGCATTTGGGGGCATCGATACTTTGGGAATAATTTTGAAAGGTTGATGGAAGTGAAAAGGAAGGTGGATCCATTTAACTTCTTCCGGCATGAGCAAAGCATACCACCAGCACCACCAAGAGTAGGAATCTAA

Protein sequence

MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPPRVGI*

Homology

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BLAST of Chy12G214410 vs. ExPASy Swiss-Prot
Match: Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.2e-35
Identity = 64/121 (52.89%), Postives = 86/121 (71.07%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           M+EISES  P PHRAG    + Y  TW++ + + +H+ W R ++N+ TP+VS++PR AY+
Sbjct: 423 MDEISESAIPFPHRAGIMYELWYTATWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYL 482

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLD+G  N +  P    +A IWG +YFG NF RL++VK K DP NFFR+EQSIPP P
Sbjct: 483 NYRDLDLGKTNPES-PNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPLP 542

Query: 121 P 122
           P
Sbjct: 543 P 542

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BLAST of Chy12G214410 vs. ExPASy Swiss-Prot
Match: A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-35
Identity = 66/120 (55.00%), Postives = 87/120 (72.50%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           M+EISES  P PHRAG    + Y  +W++ + + +HL W R ++N+MTP+VSK+PR AY+
Sbjct: 422 MDEISESAIPFPHRAGILYELWYICSWEKQEDNEKHLNWIRNIYNFMTPYVSKNPRLAYL 481

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLD+G  ND   P    +A IWG +YFG NF+RL++VK  VDP NFFR+EQSIPP P
Sbjct: 482 NYRDLDIGI-NDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPLP 540

[Close]

BLAST of Chy12G214410 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 3.5e-35
Identity = 65/122 (53.28%), Postives = 87/122 (71.31%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           M+EISES  P PHRAG    I Y  +W++ + + +H+ W R ++N+ TP+VS++PR AY+
Sbjct: 423 MDEISESAIPFPHRAGITYEIWYIASWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYL 482

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLD+G  N +  P    +A IWG +YFG NF RL++VK KVDP NFFR+EQSIPP P
Sbjct: 483 NYRDLDLGKTNFES-PNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLP 542

Query: 121 PR 123
            R
Sbjct: 543 LR 543

[Close]

BLAST of Chy12G214410 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 3.5e-35
Identity = 64/121 (52.89%), Postives = 85/121 (70.25%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           M EISES  P PHRAG    + Y  +W++ + + +H+ W R ++N+ TP+VS++PR AY+
Sbjct: 423 MEEISESAIPFPHRAGIMYELWYTASWEKQEDNEKHINWVRSVYNFTTPYVSQNPRLAYL 482

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLD+G  N    P    +A IWG +YFG NF RL++VK KVDP NFFR+EQSIPP P
Sbjct: 483 NYRDLDLGKTN-HASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLP 542

Query: 121 P 122
           P
Sbjct: 543 P 542

[Close]

BLAST of Chy12G214410 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 4.5e-35
Identity = 65/122 (53.28%), Postives = 87/122 (71.31%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           M+EISES  P PHRAG    I Y  +W++ + + +H+ W R ++N+ TP+VS++PR AY+
Sbjct: 423 MDEISESAIPFPHRAGIMYEIWYIASWEKQEDNEKHINWIRNVYNFTTPYVSQNPRMAYL 482

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLD+G  N +  P    +A IWG +YFG NF RL++VK KVDP NFFR+EQSIPP P
Sbjct: 483 NYRDLDLGKTNFES-PNNYTQARIWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPLP 542

Query: 121 PR 123
            R
Sbjct: 543 LR 543

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BLAST of Chy12G214410 vs. ExPASy TrEMBL
Match: A0A0A0LS77 (BBE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G096090 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 6.5e-69
Identity = 121/124 (97.58%), Postives = 122/124 (98.39%), Query Frame = 0


Query: 2   NEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYVN 61
           NEISESETPSPHRAGYPIHIGYYLTWQRPDADS+HLKWARELHNYMTPFVSKSPRAAYVN
Sbjct: 113 NEISESETPSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVN 172

Query: 62  YRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPP 121
           YRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 
Sbjct: 173 YRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPT 232

Query: 122 RVGI 126
            VGI
Sbjct: 233 SVGI 236

[Close]

BLAST of Chy12G214410 vs. ExPASy TrEMBL
Match: A0A5D3D0K7 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2273G00130 PE=3 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.1e-66
Identity = 116/125 (92.80%), Postives = 123/125 (98.40%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           MNEISESETPSPHRAGYPIHIGYYLTWQ+P+ADSR+L+WARELH+YMTPFVSKSPRAAYV
Sbjct: 388 MNEISESETPSPHRAGYPIHIGYYLTWQQPNADSRNLEWARELHDYMTPFVSKSPRAAYV 447

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLDMGTNNDDGVPTR +EAS+WGHRYFGNNFERLMEVK KVDPFNFFRHEQSIPPAP
Sbjct: 448 NYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAP 507

Query: 121 PRVGI 126
           PRVGI
Sbjct: 508 PRVGI 512

[Close]

BLAST of Chy12G214410 vs. ExPASy TrEMBL
Match: A0A5A7U7I2 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold60G003280 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.1e-66
Identity = 116/125 (92.80%), Postives = 123/125 (98.40%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           MNEISESETPSPHRAGYPIHIGYYLTWQ+P+ADSR+L+WARELH+YMTPFVSKSPRAAYV
Sbjct: 76  MNEISESETPSPHRAGYPIHIGYYLTWQQPNADSRNLEWARELHDYMTPFVSKSPRAAYV 135

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLDMGTNNDDGVPTR +EAS+WGHRYFGNNFERLMEVK KVDPFNFFRHEQSIPPAP
Sbjct: 136 NYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAP 195

Query: 121 PRVGI 126
           PRVGI
Sbjct: 196 PRVGI 200

[Close]

BLAST of Chy12G214410 vs. ExPASy TrEMBL
Match: A0A1S3CIZ3 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103501494 PE=3 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.1e-66
Identity = 116/125 (92.80%), Postives = 123/125 (98.40%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           MNEISESETPSPHRAGYPIHIGYYLTWQ+P+ADSR+L+WARELH+YMTPFVSKSPRAAYV
Sbjct: 388 MNEISESETPSPHRAGYPIHIGYYLTWQQPNADSRNLEWARELHDYMTPFVSKSPRAAYV 447

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLDMGTNNDDGVPTR +EAS+WGHRYFGNNFERLMEVK KVDPFNFFRHEQSIPPAP
Sbjct: 448 NYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAP 507

Query: 121 PRVGI 126
           PRVGI
Sbjct: 508 PRVGI 512

[Close]

BLAST of Chy12G214410 vs. ExPASy TrEMBL
Match: A0A6J1HYZ7 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3 SV=1)

HSP 1 Score: 227.6 bits (579), Expect = 2.8e-56
Identity = 97/125 (77.60%), Postives = 114/125 (91.20%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           MNEISE++TP+PHRA +PIHIGYY+TW RPDADSRHL+W RELH+YMTPFVS+SPRAAY+
Sbjct: 429 MNEISETDTPNPHRASHPIHIGYYVTWHRPDADSRHLEWTRELHDYMTPFVSRSPRAAYM 488

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NY+DLD+G NN+DG+PT  +EAS+WG+RYFG NFERL+EVK KVDPFNFFRHEQSIPPA 
Sbjct: 489 NYKDLDIGKNNEDGIPTSYDEASVWGYRYFGINFERLVEVKTKVDPFNFFRHEQSIPPAA 548

Query: 121 PRVGI 126
           P  GI
Sbjct: 549 PMGGI 553

[Close]

BLAST of Chy12G214410 vs. NCBI nr
Match: KAA0051883.1 (tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 260 bits (665), Expect = 1.34e-86
Identity = 116/125 (92.80%), Postives = 123/125 (98.40%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           MNEISESETPSPHRAGYPIHIGYYLTWQ+P+ADSR+L+WARELH+YMTPFVSKSPRAAYV
Sbjct: 76  MNEISESETPSPHRAGYPIHIGYYLTWQQPNADSRNLEWARELHDYMTPFVSKSPRAAYV 135

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLDMGTNNDDGVPTR +EAS+WGHRYFGNNFERLMEVK KVDPFNFFRHEQSIPPAP
Sbjct: 136 NYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAP 195

Query: 121 PRVGI 125
           PRVGI
Sbjct: 196 PRVGI 200

[Close]

BLAST of Chy12G214410 vs. NCBI nr
Match: XP_004147307.2 (berberine bridge enzyme-like 27 [Cucumis sativus])

HSP 1 Score: 270 bits (690), Expect = 6.20e-86
Identity = 121/124 (97.58%), Postives = 122/124 (98.39%), Query Frame = 0


Query: 2   NEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYVN 61
           NEISESETPSPHRAGYPIHIGYYLTWQRPDADS+HLKWARELHNYMTPFVSKSPRAAYVN
Sbjct: 418 NEISESETPSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVN 477

Query: 62  YRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPP 121
           YRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 
Sbjct: 478 YRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPT 537

Query: 122 RVGI 125
            VGI
Sbjct: 538 SVGI 541

[Close]

BLAST of Chy12G214410 vs. NCBI nr
Match: KAE8652747.1 (hypothetical protein Csa_013703 [Cucumis sativus])

HSP 1 Score: 270 bits (690), Expect = 1.64e-83
Identity = 121/124 (97.58%), Postives = 122/124 (98.39%), Query Frame = 0


Query: 2   NEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYVN 61
           NEISESETPSPHRAGYPIHIGYYLTWQRPDADS+HLKWARELHNYMTPFVSKSPRAAYVN
Sbjct: 677 NEISESETPSPHRAGYPIHIGYYLTWQRPDADSKHLKWARELHNYMTPFVSKSPRAAYVN 736

Query: 62  YRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPP 121
           YRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 
Sbjct: 737 YRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAPT 796

Query: 122 RVGI 125
            VGI
Sbjct: 797 SVGI 800

[Close]

BLAST of Chy12G214410 vs. NCBI nr
Match: XP_008463307.2 (PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >TYK17150.1 tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa])

HSP 1 Score: 260 bits (665), Expect = 1.66e-82
Identity = 116/125 (92.80%), Postives = 123/125 (98.40%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           MNEISESETPSPHRAGYPIHIGYYLTWQ+P+ADSR+L+WARELH+YMTPFVSKSPRAAYV
Sbjct: 388 MNEISESETPSPHRAGYPIHIGYYLTWQQPNADSRNLEWARELHDYMTPFVSKSPRAAYV 447

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLDMGTNNDDGVPTR +EAS+WGHRYFGNNFERLMEVK KVDPFNFFRHEQSIPPAP
Sbjct: 448 NYRDLDMGTNNDDGVPTRYKEASLWGHRYFGNNFERLMEVKMKVDPFNFFRHEQSIPPAP 507

Query: 121 PRVGI 125
           PRVGI
Sbjct: 508 PRVGI 512

[Close]

BLAST of Chy12G214410 vs. NCBI nr
Match: XP_038895067.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 236 bits (602), Expect = 8.30e-73
Identity = 105/125 (84.00%), Postives = 113/125 (90.40%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDADSRHLKWARELHNYMTPFVSKSPRAAYV 60
           MNEISESETPS HRAGYPIHIGYY+ WQ PDADSRHL+W REL+NYMTPFVSKSPRAAY 
Sbjct: 414 MNEISESETPSAHRAGYPIHIGYYVKWQHPDADSRHLEWTRELYNYMTPFVSKSPRAAYA 473

Query: 61  NYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPAP 120
           NYRDLD+GTNN+D + T  EEAS+WGH+YFGNNFERLMEVK KVD FNFFRHEQSIPPAP
Sbjct: 474 NYRDLDIGTNNEDRILTSYEEASVWGHQYFGNNFERLMEVKAKVDQFNFFRHEQSIPPAP 533

Query: 121 PRVGI 125
           PRV I
Sbjct: 534 PRVRI 538

[Close]

BLAST of Chy12G214410 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 143.7 bits (361), Expect = 1.0e-34
Identity = 67/119 (56.30%), Postives = 82/119 (68.91%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPD-ADSRHLKWARELHNYMTPFVSKSPRAAY 60
           M +I ES+ P PHR G    + Y  +W   D   SRH+ W R+L++YMTP+VS +PR AY
Sbjct: 418 MAKIPESQIPFPHRKGVLFKVQYVTSWLDSDKRPSRHINWIRDLYSYMTPYVSSNPREAY 477

Query: 61  VNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 119
           VNYRDLD+G N  D V T  ++A +WG  YF NNF RLM +K KVDP NFFRHEQSIPP
Sbjct: 478 VNYRDLDLGRNTKD-VKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPP 535

[Close]

BLAST of Chy12G214410 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 139.4 bits (350), Expect = 1.9e-33
Identity = 67/121 (55.37%), Postives = 81/121 (66.94%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPD-ADSRHLKWARELHNYMTPFVSKSPRAAY 60
           M++I ESE P PHR G    I YY +W   +   +R  KW REL+ YMTP+VS +PR AY
Sbjct: 422 MDKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKWIRELYGYMTPYVSSNPRQAY 481

Query: 61  VNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPA 120
           VNYRDLD+G N D+   +   EA IWG  YF +NF RL+ +K KVDP NFFRHEQSIP  
Sbjct: 482 VNYRDLDLGQNKDNS-KSNFIEAKIWGANYFKDNFNRLVRIKSKVDPDNFFRHEQSIPTL 541

[Close]

BLAST of Chy12G214410 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 138.7 bits (348), Expect = 3.3e-33
Identity = 65/121 (53.72%), Postives = 80/121 (66.12%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPD-ADSRHLKWARELHNYMTPFVSKSPRAAY 60
           M++I ES  P PHR G    I YY  W   D   SR + W +E++NYM P+VS +PR AY
Sbjct: 421 MSKIPESAIPFPHRNGTLFKILYYANWLENDKTSSRKINWIKEIYNYMAPYVSSNPRQAY 480

Query: 61  VNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPPA 120
           VNYRDLD G N ++       EA IWG +YF  NF+RL+++K KVDP NFFRHEQSIPP 
Sbjct: 481 VNYRDLDFGQNKNN-AKVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 540

[Close]

BLAST of Chy12G214410 vs. TAIR 10
Match: AT4G20860.1 (FAD-binding Berberine family protein )

HSP 1 Score: 137.9 bits (346), Expect = 5.7e-33
Identity = 63/120 (52.50%), Postives = 87/120 (72.50%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDAD--SRHLKWARELHNYMTPFVSKSPRAA 60
           +++ISE+E+P PHR G   +I Y + W+  + +  ++H++W R LH+YMTP+VSKSPR A
Sbjct: 411 ISKISETESPYPHRRGNLYNIQYMVKWKVNEVEEMNKHVRWMRSLHDYMTPYVSKSPRGA 470

Query: 61  YVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 119
           Y+NYRDLD+G+    G+ T  E+A  WG  YF  NF+RL  VK K+DP NFFR+EQSIPP
Sbjct: 471 YLNYRDLDLGSTK--GINTSFEDARKWGETYFKGNFKRLGLVKGKIDPTNFFRNEQSIPP 528

[Close]

BLAST of Chy12G214410 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 137.1 bits (344), Expect = 9.7e-33
Identity = 65/120 (54.17%), Postives = 82/120 (68.33%), Query Frame = 0


Query: 1   MNEISESETPSPHRAGYPIHIGYYLTWQRPDAD--SRHLKWARELHNYMTPFVSKSPRAA 60
           M EI E+ETP PHR G   +I Y + W+  D     +H+ W R L+ YM  +VS SPR A
Sbjct: 412 MYEIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGA 471

Query: 61  YVNYRDLDMGTNNDDGVPTRCEEASIWGHRYFGNNFERLMEVKRKVDPFNFFRHEQSIPP 119
           Y+NYRDLD+G N   GV T  E+A +WG RYFG+NF+RL  VK K+DP NFFR+EQS+PP
Sbjct: 472 YLNYRDLDLGMNR--GVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPP 529

The following BLAST results are available for this feature:

BLAST of Chy12G214410 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q33DQ2	1.2e-35	52.89	Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1	[more]
A6P6V9	1.6e-35	55.00	Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1	[more]
A6P6W1	3.5e-35	53.28	Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=...	[more]
Q8GTB6	3.5e-35	52.89	Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV...	[more]
A6P6W0	4.5e-35	53.28	Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=...	[more]

BLAST of Chy12G214410 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LS77	6.5e-69	97.58	BBE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G096090 PE=4 SV...	[more]
A0A5D3D0K7	5.1e-66	92.80	Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A5A7U7I2	5.1e-66	92.80	Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695...	[more]
A0A1S3CIZ3	5.1e-66	92.80	tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC10350149...	[more]
A0A6J1HYZ7	2.8e-56	77.60	berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111469396 PE=3...	[more]

BLAST of Chy12G214410 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0051883.1	1.34e-86	92.80	tetrahydrocannabinolic acid synthase-like [Cucumis melo var. makuwa]	[more]
XP_004147307.2	6.20e-86	97.58	berberine bridge enzyme-like 27 [Cucumis sativus]	[more]
KAE8652747.1	1.64e-83	97.58	hypothetical protein Csa_013703 [Cucumis sativus]	[more]
XP_008463307.2	1.66e-82	92.80	PREDICTED: tetrahydrocannabinolic acid synthase-like [Cucumis melo] >TYK17150.1 ...	[more]
XP_038895067.1	8.30e-73	84.00	berberine bridge enzyme-like 18 [Benincasa hispida]	[more]

BLAST of Chy12G214410 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G44400.1	1.0e-34	56.30	FAD-binding Berberine family protein	[more]
AT5G44390.1	1.9e-33	55.37	FAD-binding Berberine family protein	[more]
AT5G44380.1	3.3e-33	53.72	FAD-binding Berberine family protein	[more]
AT4G20860.1	5.7e-33	52.50	FAD-binding Berberine family protein	[more]
AT5G44360.1	9.7e-33	54.17	FAD-binding Berberine family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.462.20		coord: 1..54 e-value: 6.4E-46 score: 159.4
None	No IPR available	PANTHER	PTHR32448	OS08G0158400 PROTEIN	coord: 1..118
None	No IPR available	PANTHER	PTHR32448:SF113	BERBERINE BRIDGE ENZYME-LIKE 18	coord: 1..118
IPR012951	Berberine/berberine-like	PFAM	PF08031	BBE	coord: 58..117 e-value: 1.5E-23 score: 82.6
IPR016169	FAD-binding, type PCMH, subdomain 2	GENE3D	3.30.465.10		coord: 55..118 e-value: 6.4E-46 score: 159.4

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G214410.1	Chy12G214410.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0050660	flavin adenine dinucleotide binding