Chy12G214400 (gene) Cucumber (hystrix) v1

Overview


Name	Chy12G214400
Type	gene
Organism	Cucumis hystrix (Cucumber (hystrix) v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like
Location	chrH12: 13670267 .. 13671988 (+)
RNA-Seq Expression	Chy12G214400
Synteny	Chy12G214400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATCAGAAGCCTGGGCTTTCATCAAACAAGTGGTTGAAGGACGATGGTTTTCGGTATGGGCGGGTATGATGATGATGATCGGTAACGGTACAACATACATTTTCGGAACATACTCCAAAGTCCTCAAGTCAGAATTTGATTACAGCCAAACCCAAGTTAACATGTTAGGATTTGCTAAAGATCTCGGTAACAATGCTGGGATTATCGCGGGTCTACTCTCCGAGTTCGTTCCAACGTGGGTTCTCTTCATGATCGGCGCATTCCAAAACTTCACTGGCTACTTCTTGATATGGCTATCCATGACACGCCGGATCAGTCAACCAGCATTTTGGCAAATGTTTCTTTGCGTTTGCTTTGGTTCTAATTCTTCTAATTATTCCAATACAGCCATTATGGTCACTAGTCTTAGAAATTTCCCGGACCGCCGTGGTATCATTTTAGGCTTACTCAAAGGGTATGTTGGTATTGGAGGTGCCATTTTAACCCAAGTTTGTTTAGGTCTTTATGGCCCTGAAGATCCATCAAATATTGTTCTTCTTTTCGCTTGGTTTCCCTCAGTCCTAATCCTTCTTATTTCCCATTCAATTCGACCAATTCTTATCCGTAAGCATCCAGAAGAGCTCAAAGTTTTTTACCATTTACTTTATGTTTCTATTGTTCTTGCAATCTTCATCTTGTTTTTGACCATGTCACAAAAACAAGTTATTTTTTCTCAGTCTGCTTATGCGAGTGGCGCCTCCGTCGTCGTGGCCTTACTCTTTTTACCTCTTTTGATTGCTTGTAGAGAAGAATTCTTATTGTACAAACTCAAGAAACAAAACCATAACCTTGATCCTTCTGTCACCCTCTCCATTATTGATCAAAAGGTACCAAACAGTCACAAACCATTTTCTACATTGGAAGAAATTGCAGAGATTTCTCCGTCTTGTTTGTCGAACATATGTAACAAACCACATCGAGGCGACGATTTCACCATCTTACAAGCTGTCTTCAGTATAGATATGGTTTTAATTTGTTTAGCTACTTTTGCTGGATGTGGGTCTTCTCTTGCAGCCATTGATAATTTAGGTCAAATTGGCGAATCACTCGGCTACCCACCACAAGCCATAGGCATCTTCGTTTCTTGGGTTTCGATATTCAATTTCTTCGGCCGAGTCGTCTCTGGTTTCATCTCAGAATTGATGATGATCAAATACAAATTACCTCGTCCTCTAATGTTTGCCTTAGCCTTTCTTCTCACTTGCATTGGCCAGCTTTGTATAGCGTATCCGTTTCCAGGTTCCCTGTATGTAGCTTCCATCGTTATCGGATTCGGATTCGGAGCACAAAATCCATTGCTGTTCGCGGTAATCTCAGAGATGTTTGGCCTAAAACATTACTCCATATTGTTCAATTGTGGGCAATTGGCAGTCCCATTAGGGTCTTACATACTAAATGTGGATATAGTTGGGAAACTGTACGACGCAGAAGCGTTACGTGAAGGTAAAAATACGACGGGCAGAGGAGTAAATTGCAGTGGAGCTCATTGTTTTGGTGGATCTTTTACAATTTTGGCTGCATTCACATTGTTTGGAGCATTGGTAATGCTTGTATTGGCTTATAGGACAAGAGACTATTATAGATGGGATGTATATAAGAATTACAAAGAGGATATGTGGATTCCTCAAGCAGAGATGGAATTTTATCGCTTAGACAACAAGAAGAACATTGATGACTAA

mRNA sequence

ATGGGATCAGAAGCCTGGGCTTTCATCAAACAAGTGGTTGAAGGACGATGGTTTTCGGTATGGGCGGGTATGATGATGATGATCGGTAACGGTACAACATACATTTTCGGAACATACTCCAAAGTCCTCAAGTCAGAATTTGATTACAGCCAAACCCAAGTTAACATGTTAGGATTTGCTAAAGATCTCGGTAACAATGCTGGGATTATCGCGGGTCTACTCTCCGAGTTCGTTCCAACGTGGGTTCTCTTCATGATCGGCGCATTCCAAAACTTCACTGGCTACTTCTTGATATGGCTATCCATGACACGCCGGATCAGTCAACCAGCATTTTGGCAAATGTTTCTTTGCGTTTGCTTTGGTTCTAATTCTTCTAATTATTCCAATACAGCCATTATGGTCACTAGTCTTAGAAATTTCCCGGACCGCCGTGGTATCATTTTAGGCTTACTCAAAGGGTATGTTGGTATTGGAGGTGCCATTTTAACCCAAGTTTGTTTAGGTCTTTATGGCCCTGAAGATCCATCAAATATTGTTCTTCTTTTCGCTTGGTTTCCCTCAGTCCTAATCCTTCTTATTTCCCATTCAATTCGACCAATTCTTATCCGTAAGCATCCAGAAGAGCTCAAAGTTTTTTACCATTTACTTTATGTTTCTATTGTTCTTGCAATCTTCATCTTGTTTTTGACCATGTCACAAAAACAAGTTATTTTTTCTCAGTCTGCTTATGCGAGTGGCGCCTCCGTCGTCGTGGCCTTACTCTTTTTACCTCTTTTGATTGCTTGTAGAGAAGAATTCTTATTGTACAAACTCAAGAAACAAAACCATAACCTTGATCCTTCTGTCACCCTCTCCATTATTGATCAAAAGGTACCAAACAGTCACAAACCATTTTCTACATTGGAAGAAATTGCAGAGATTTCTCCGTCTTGTTTGTCGAACATATGTAACAAACCACATCGAGGCGACGATTTCACCATCTTACAAGCTGTCTTCAGTATAGATATGGTTTTAATTTGTTTAGCTACTTTTGCTGGATGTGGGTCTTCTCTTGCAGCCATTGATAATTTAGGTCAAATTGGCGAATCACTCGGCTACCCACCACAAGCCATAGGCATCTTCGTTTCTTGGGTTTCGATATTCAATTTCTTCGGCCGAGTCGTCTCTGGTTTCATCTCAGAATTGATGATGATCAAATACAAATTACCTCGTCCTCTAATGTTTGCCTTAGCCTTTCTTCTCACTTGCATTGGCCAGCTTTGTATAGCGTATCCGTTTCCAGGTTCCCTGTATGTAGCTTCCATCGTTATCGGATTCGGATTCGGAGCACAAAATCCATTGCTGTTCGCGGTAATCTCAGAGATGTTTGGCCTAAAACATTACTCCATATTGTTCAATTGTGGGCAATTGGCAGTCCCATTAGGGTCTTACATACTAAATGTGGATATAGTTGGGAAACTGTACGACGCAGAAGCGTTACGTGAAGGTAAAAATACGACGGGCAGAGGAGTAAATTGCAGTGGAGCTCATTGTTTTGGTGGATCTTTTACAATTTTGGCTGCATTCACATTGTTTGGAGCATTGGTAATGCTTGTATTGGCTTATAGGACAAGAGACTATTATAGATGGGATGTATATAAGAATTACAAAGAGGATATGTGGATTCCTCAAGCAGAGATGGAATTTTATCGCTTAGACAACAAGAAGAACATTGATGACTAA

Coding sequence (CDS)

ATGGGATCAGAAGCCTGGGCTTTCATCAAACAAGTGGTTGAAGGACGATGGTTTTCGGTATGGGCGGGTATGATGATGATGATCGGTAACGGTACAACATACATTTTCGGAACATACTCCAAAGTCCTCAAGTCAGAATTTGATTACAGCCAAACCCAAGTTAACATGTTAGGATTTGCTAAAGATCTCGGTAACAATGCTGGGATTATCGCGGGTCTACTCTCCGAGTTCGTTCCAACGTGGGTTCTCTTCATGATCGGCGCATTCCAAAACTTCACTGGCTACTTCTTGATATGGCTATCCATGACACGCCGGATCAGTCAACCAGCATTTTGGCAAATGTTTCTTTGCGTTTGCTTTGGTTCTAATTCTTCTAATTATTCCAATACAGCCATTATGGTCACTAGTCTTAGAAATTTCCCGGACCGCCGTGGTATCATTTTAGGCTTACTCAAAGGGTATGTTGGTATTGGAGGTGCCATTTTAACCCAAGTTTGTTTAGGTCTTTATGGCCCTGAAGATCCATCAAATATTGTTCTTCTTTTCGCTTGGTTTCCCTCAGTCCTAATCCTTCTTATTTCCCATTCAATTCGACCAATTCTTATCCGTAAGCATCCAGAAGAGCTCAAAGTTTTTTACCATTTACTTTATGTTTCTATTGTTCTTGCAATCTTCATCTTGTTTTTGACCATGTCACAAAAACAAGTTATTTTTTCTCAGTCTGCTTATGCGAGTGGCGCCTCCGTCGTCGTGGCCTTACTCTTTTTACCTCTTTTGATTGCTTGTAGAGAAGAATTCTTATTGTACAAACTCAAGAAACAAAACCATAACCTTGATCCTTCTGTCACCCTCTCCATTATTGATCAAAAGGTACCAAACAGTCACAAACCATTTTCTACATTGGAAGAAATTGCAGAGATTTCTCCGTCTTGTTTGTCGAACATATGTAACAAACCACATCGAGGCGACGATTTCACCATCTTACAAGCTGTCTTCAGTATAGATATGGTTTTAATTTGTTTAGCTACTTTTGCTGGATGTGGGTCTTCTCTTGCAGCCATTGATAATTTAGGTCAAATTGGCGAATCACTCGGCTACCCACCACAAGCCATAGGCATCTTCGTTTCTTGGGTTTCGATATTCAATTTCTTCGGCCGAGTCGTCTCTGGTTTCATCTCAGAATTGATGATGATCAAATACAAATTACCTCGTCCTCTAATGTTTGCCTTAGCCTTTCTTCTCACTTGCATTGGCCAGCTTTGTATAGCGTATCCGTTTCCAGGTTCCCTGTATGTAGCTTCCATCGTTATCGGATTCGGATTCGGAGCACAAAATCCATTGCTGTTCGCGGTAATCTCAGAGATGTTTGGCCTAAAACATTACTCCATATTGTTCAATTGTGGGCAATTGGCAGTCCCATTAGGGTCTTACATACTAAATGTGGATATAGTTGGGAAACTGTACGACGCAGAAGCGTTACGTGAAGGTAAAAATACGACGGGCAGAGGAGTAAATTGCAGTGGAGCTCATTGTTTTGGTGGATCTTTTACAATTTTGGCTGCATTCACATTGTTTGGAGCATTGGTAATGCTTGTATTGGCTTATAGGACAAGAGACTATTATAGATGGGATGTATATAAGAATTACAAAGAGGATATGTGGATTCCTCAAGCAGAGATGGAATTTTATCGCTTAGACAACAAGAAGAACATTGATGACTAA

Protein sequence

MGSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLLFAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQSAYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTLEEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIGESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLCIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNVDIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYYRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD*

Homology

[Close]

BLAST of Chy12G214400 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 137.9 bits (346), Expect = 3.6e-31
Identity = 145/540 (26.85%), Postives = 232/540 (42.96%), Query Frame = 0


Query: 16  RWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAGIIAGLLS 75
           +W  + A + +    GT + F  YS  LKS    SQ ++N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 76  EFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYSNTAIMVT 135
            + P  V+    A   F GY + WL +T  I+ P +  +FLC      S  + NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 136 SLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLLFAWFPSVLILLISH 195
            +R+FP+ R + L L   + GI  A L  +      P   +  +LL +  P V+     +
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAA-LYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALY 222

Query: 196 SI--RPIL-------IRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQSAYASG 255
            +  +P L        R+H   +   + +L V  V+  F L L+ S      + SA  + 
Sbjct: 223 PVLTKPSLDTTPDYDSRRHDSHV---FTILNVLAVITSFHLLLSSSS-----TSSARLNF 282

Query: 256 ASVVVALLF---LPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTLEE 315
              VV L+F    PLL+  R+ FL     + NH     V L+I + K   +     T  E
Sbjct: 283 IGAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYE 342

Query: 316 IAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIGES 375
               +        N    GD+ +    +  ++  L  +A F G    L   +NLGQI +S
Sbjct: 343 HMGTAKE-----GNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQS 402

Query: 376 LGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIG--QLC 435
           LG   Q     V+  S F+FFGR++S    + M  +++L R   FA+A L T I    L 
Sbjct: 403 LG---QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLA 462

Query: 436 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 495
           ++     +L  A+ +IG   G       ++ S++FG     +  N     +P+GS +   
Sbjct: 463 VSSSQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY 522

Query: 496 DIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYY 542
            I   +Y+A A  +        + C G  C+  +F      ++ G +  L L  RT+  Y
Sbjct: 523 -IAASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

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BLAST of Chy12G214400 vs. ExPASy TrEMBL
Match: A0A1S3CMS0 (uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=4 SV=1)

HSP 1 Score: 1007.3 bits (2603), Expect = 2.6e-290
Identity = 502/573 (87.61%), Postives = 537/573 (93.72%), Query Frame = 0


Query: 1   MGSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA 60
           M SEAWAFIKQVV+GRWFSV+AGMMMMIGNGTTYIFGTYSKVLK+EFDYSQTQVNMLGFA
Sbjct: 1   MESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFA 60

Query: 61  KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF 120
           KDLG+N GIIAGLL+EFVPTWVLF IGAFQNFT YF IWLSMTRRI QP FWQMFL VCF
Sbjct: 61  KDLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCF 120

Query: 121 GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVL 180
           GSNSSNY+NTAIMV SLRNFPDRRGIILGLLKGYVGIGGAILTQ+ LG+YGPEDPS++VL
Sbjct: 121 GSNSSNYANTAIMVISLRNFPDRRGIILGLLKGYVGIGGAILTQIYLGIYGPEDPSDLVL 180

Query: 181 LFAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQ 240
           LFAWFPSVLILLIS SIRPI IRKHPEELKVFYHLLYVSI++AIFI FL+++QKQV FS 
Sbjct: 181 LFAWFPSVLILLISTSIRPIHIRKHPEELKVFYHLLYVSIIIAIFIFFLSITQKQVTFSP 240

Query: 241 SAYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFST 300
           +AYASGA VVVALL LPLLI CREEFLLYKLKKQN N DPSV LSI+DQKV N  K FST
Sbjct: 241 AAYASGAYVVVALLCLPLLIVCREEFLLYKLKKQNLNRDPSVALSIVDQKVTNCPKSFST 300

Query: 301 LEEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQI 360
           LEEIAEISPSC+SNICNKP RG+DFT+LQA+FSIDM+LICLATF+GCGSSLAAIDNLGQI
Sbjct: 301 LEEIAEISPSCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSSLAAIDNLGQI 360

Query: 361 GESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQL 420
           GESLGYP +AI IFVSWVSIFNFFGRV SGFISELM+IKYKLPRPLMFA AFLLTC+GQL
Sbjct: 361 GESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAFAFLLTCLGQL 420

Query: 421 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 480
           CIAYPFPGS+YVAS +IGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQL VPLGSYILN
Sbjct: 421 CIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLVVPLGSYILN 480

Query: 481 VDIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDY 540
           VDIVGKLYDAEALREG+  TG+G+ C+GAHCFGGSFTILAA TLFGALVML+LAYRTRDY
Sbjct: 481 VDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVMLLLAYRTRDY 540

Query: 541 YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 574
           YRWD+YKNYKEDMWIPQAEMEFYRLDN+KNIDD
Sbjct: 541 YRWDIYKNYKEDMWIPQAEMEFYRLDNRKNIDD 573

[Close]

BLAST of Chy12G214400 vs. ExPASy TrEMBL
Match: A0A6J1HLS6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464776 PE=4 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 6.9e-251
Identity = 436/572 (76.22%), Postives = 499/572 (87.24%), Query Frame = 0


Query: 2   GSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAWAFIKQVV GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L +YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQS 241
           FAW PS+LIL++  SIRPI IRKHP+ELKVFY LLYVSI+LAIFILFLT++QKQV+FSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS VV LL +P+LIACREE LLYKL K+     PSV LS++ Q  P    P S  
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKE--TAAPSVALSMLHQNPPT--LPTSAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RG+DFTILQAVFS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLC 421
           ESLGY  +AIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL FA AFL+TC+GQL 
Sbjct: 366 ESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGSLY+A+I+IGFGFGAQNP+LFAVISEMFGLK YS LFNCGQLA P GSYILNV
Sbjct: 426 IAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILNV 485

Query: 482 DIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYY 541
           D+VGKLYD EALRE K   G+G+NC+GAHCFGGSFT+LAA TLFGA+VM VLAYRTR++Y
Sbjct: 486 DVVGKLYDMEALRERKQIAGKGLNCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 574
           + DVYKN+ E++WIPQ EMEFYRLDNKKNI+D
Sbjct: 546 KRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Chy12G214400 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 2.0e-250
Identity = 434/572 (75.87%), Postives = 501/572 (87.59%), Query Frame = 0


Query: 2   GSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAW FIKQVV GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNF G+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q  L +YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQS 241
           FAW PS+LIL++  SIRPI IRKHP ELKVFY LLYVSI+LAIFILFLT++QKQV+FSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS VV+LL +P+LIACREE LLYKL KQ     PSVT+S++ Q  P    P S  
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQ--TAGPSVTVSMVHQNPPT--LPASAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RG+DFTILQAVFS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLC 421
           ESLGYP +AIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL F+ AFL+TC+GQL 
Sbjct: 366 ESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGS+Y+A+I+IGFGFGAQNP+LFAVISEMFGL+ YS LFNCGQLAVPLGSYILNV
Sbjct: 426 IAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILNV 485

Query: 482 DIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYY 541
           D+VGKLYD EALREGK   G+G+ C+GAHCFGGSFTILAA TLFGA+VMLVLAYRTR++Y
Sbjct: 486 DVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 574
           + DVYKN+ E++WIPQ +MEFYRLD+KK+I+D
Sbjct: 546 KRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

[Close]

BLAST of Chy12G214400 vs. ExPASy TrEMBL
Match: A0A6J1HLT1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464780 PE=4 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 3.4e-250
Identity = 435/572 (76.05%), Postives = 498/572 (87.06%), Query Frame = 0


Query: 2   GSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAWAFIKQVV GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L +YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQS 241
           FAW PS+LIL++  SIRPI IRKHP+ELKVFY LLYVSI+LAIFILFLT++QKQV+FSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS VV LL +P+LIACREE LLYKL K+     PSV LS++ Q  P    P S  
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKE--TAAPSVALSMLHQNPPT--LPTSAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RG+DFTILQAVFS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLC 421
           ESLGY  +AIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL FA AFL+TC+GQL 
Sbjct: 366 ESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGSLY+A+I+IGFGFGAQNP+LFAVISEMFGLK YS LFNCGQLA P GSYILNV
Sbjct: 426 IAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILNV 485

Query: 482 DIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYY 541
           D+VGKLYD EALRE K   G+G+ C+GAHCFGGSFT+LAA TLFGA+VM VLAYRTR++Y
Sbjct: 486 DVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 574
           + DVYKN+ E++WIPQ EMEFYRLDNKKNI+D
Sbjct: 546 KRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Chy12G214400 vs. ExPASy TrEMBL
Match: A0A6J1I2B3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469248 PE=4 SV=1)

HSP 1 Score: 873.2 bits (2255), Expect = 5.8e-250
Identity = 432/572 (75.52%), Postives = 499/572 (87.24%), Query Frame = 0


Query: 2   GSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAW FIKQVV GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF++GAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L +YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQS 241
           FAW PS+LIL++  SIRPI IRKHP ELKVFY LLYVSI+LAIFILFLT++QKQV+FSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS VV+LL +P+LIACREE LLYKL KQ     PSVT+S++ Q  P      S  
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQ--TAGPSVTVSMVHQNPPTLSA--SAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EI PSC  NI NKP RG+DFTILQAVFS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLC 421
           ESLGYP +AIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL F+ AFL+TC+GQL 
Sbjct: 366 ESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGS+Y A+I+IGFGFGAQNP+LFAVISEMFGL+HYS LFNCGQL VPLGSYILNV
Sbjct: 426 IAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSYILNV 485

Query: 482 DIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYY 541
           D+VGKLYD EAL EGK   G+G+ C+GAHCFGGSFTILAA TLFGA+VMLVLAYRTR++Y
Sbjct: 486 DVVGKLYDMEALHEGKQIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 574
           + DVYKN+ E++WIPQ +MEFYRLDNKK+I+D
Sbjct: 546 KRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

[Close]

BLAST of Chy12G214400 vs. NCBI nr
Match: XP_004147308.1 (uncharacterized protein LOC101204150 [Cucumis sativus] >KAE8652748.1 hypothetical protein Csa_014134 [Cucumis sativus])

HSP 1 Score: 1111 bits (2874), Expect = 0.0
Identity = 554/573 (96.68%), Postives = 566/573 (98.78%), Query Frame = 0


Query: 1   MGSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA 60
           MGSEAWAFIKQV EGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA
Sbjct: 1   MGSEAWAFIKQVAEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA 60

Query: 61  KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF 120
           KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF
Sbjct: 61  KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF 120

Query: 121 GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVL 180
           GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQ+CLG YGPEDPSNIVL
Sbjct: 121 GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQICLGFYGPEDPSNIVL 180

Query: 181 LFAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQ 240
           LFAWFPSVLILLIS+SIRPI IRKHPEELKVFYHLLYVSIVLAIFILFLTMS+KQV+FSQ
Sbjct: 181 LFAWFPSVLILLISNSIRPIHIRKHPEELKVFYHLLYVSIVLAIFILFLTMSEKQVVFSQ 240

Query: 241 SAYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFST 300
           SAYASGASVV+ALLFLPLLIACREEFLLYKLKKQNHNL+PSVTLSIIDQKVPNSHKPFST
Sbjct: 241 SAYASGASVVIALLFLPLLIACREEFLLYKLKKQNHNLEPSVTLSIIDQKVPNSHKPFST 300

Query: 301 LEEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQI 360
           LEEIAEISPSCLSNICNKPHRG+DFTILQA+FS+DMVLICLATFAGCGSSLAAIDNLGQI
Sbjct: 301 LEEIAEISPSCLSNICNKPHRGEDFTILQAIFSVDMVLICLATFAGCGSSLAAIDNLGQI 360

Query: 361 GESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQL 420
           GESLGYPP+AIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFA AFLLTCIGQL
Sbjct: 361 GESLGYPPRAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFAFAFLLTCIGQL 420

Query: 421 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 480
           CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN
Sbjct: 421 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 480

Query: 481 VDIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDY 540
           VDIVGKLYDAEALREGK  TGRG+NCSGAHCFGGSFTILAA TLFGALVMLVLAYRTR+Y
Sbjct: 481 VDIVGKLYDAEALREGKKMTGRGINCSGAHCFGGSFTILAASTLFGALVMLVLAYRTREY 540

Query: 541 YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD
Sbjct: 541 YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573

[Close]

BLAST of Chy12G214400 vs. NCBI nr
Match: XP_008464773.1 (PREDICTED: uncharacterized protein LOC103502580 [Cucumis melo])

HSP 1 Score: 1008 bits (2606), Expect = 0.0
Identity = 502/573 (87.61%), Postives = 537/573 (93.72%), Query Frame = 0


Query: 1   MGSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFA 60
           M SEAWAFIKQVV+GRWFSV+AGMMMMIGNGTTYIFGTYSKVLK+EFDYSQTQVNMLGFA
Sbjct: 1   MESEAWAFIKQVVQGRWFSVYAGMMMMIGNGTTYIFGTYSKVLKTEFDYSQTQVNMLGFA 60

Query: 61  KDLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCF 120
           KDLG+N GIIAGLL+EFVPTWVLF IGAFQNFT YF IWLSMTRRI QP FWQMFL VCF
Sbjct: 61  KDLGSNVGIIAGLLAEFVPTWVLFTIGAFQNFTCYFFIWLSMTRRIPQPEFWQMFLSVCF 120

Query: 121 GSNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVL 180
           GSNSSNY+NTAIMV SLRNFPDRRGIILGLLKGYVGIGGAILTQ+ LG+YGPEDPS++VL
Sbjct: 121 GSNSSNYANTAIMVISLRNFPDRRGIILGLLKGYVGIGGAILTQIYLGIYGPEDPSDLVL 180

Query: 181 LFAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQ 240
           LFAWFPSVLILLIS SIRPI IRKHPEELKVFYHLLYVSI++AIFI FL+++QKQV FS 
Sbjct: 181 LFAWFPSVLILLISTSIRPIHIRKHPEELKVFYHLLYVSIIIAIFIFFLSITQKQVTFSP 240

Query: 241 SAYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFST 300
           +AYASGA VVVALL LPLLI CREEFLLYKLKKQN N DPSV LSI+DQKV N  K FST
Sbjct: 241 AAYASGAYVVVALLCLPLLIVCREEFLLYKLKKQNLNRDPSVALSIVDQKVTNCPKSFST 300

Query: 301 LEEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQI 360
           LEEIAEISPSC+SNICNKP RG+DFT+LQA+FSIDM+LICLATF+GCGSSLAAIDNLGQI
Sbjct: 301 LEEIAEISPSCISNICNKPDRGNDFTVLQAIFSIDMLLICLATFSGCGSSLAAIDNLGQI 360

Query: 361 GESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQL 420
           GESLGYP +AI IFVSWVSIFNFFGRV SGFISELM+IKYKLPRPLMFA AFLLTC+GQL
Sbjct: 361 GESLGYPARAISIFVSWVSIFNFFGRVFSGFISELMIIKYKLPRPLMFAFAFLLTCLGQL 420

Query: 421 CIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILN 480
           CIAYPFPGS+YVAS +IGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQL VPLGSYILN
Sbjct: 421 CIAYPFPGSVYVASSIIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLVVPLGSYILN 480

Query: 481 VDIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDY 540
           VDIVGKLYDAEALREG+  TG+G+ C+GAHCFGGSFTILAA TLFGALVML+LAYRTRDY
Sbjct: 481 VDIVGKLYDAEALREGRKMTGKGITCTGAHCFGGSFTILAASTLFGALVMLLLAYRTRDY 540

Query: 541 YRWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           YRWD+YKNYKEDMWIPQAEMEFYRLDN+KNIDD
Sbjct: 541 YRWDIYKNYKEDMWIPQAEMEFYRLDNRKNIDD 573

[Close]

BLAST of Chy12G214400 vs. NCBI nr
Match: XP_022964775.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 877 bits (2266), Expect = 0.0
Identity = 436/572 (76.22%), Postives = 499/572 (87.24%), Query Frame = 0


Query: 2   GSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAWAFIKQVV GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L +YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQS 241
           FAW PS+LIL++  SIRPI IRKHP+ELKVFY LLYVSI+LAIFILFLT++QKQV+FSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS VV LL +P+LIACREE LLYKL K+     PSV LS++ Q  P    P S  
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKET--AAPSVALSMLHQNPPTL--PTSAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RG+DFTILQAVFS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLC 421
           ESLGY  +AIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL FA AFL+TC+GQL 
Sbjct: 366 ESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGSLY+A+I+IGFGFGAQNP+LFAVISEMFGLK YS LFNCGQLA P GSYILNV
Sbjct: 426 IAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILNV 485

Query: 482 DIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYY 541
           D+VGKLYD EALRE K   G+G+NC+GAHCFGGSFT+LAA TLFGA+VM VLAYRTR++Y
Sbjct: 486 DVVGKLYDMEALRERKQIAGKGLNCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ EMEFYRLDNKKNI+D
Sbjct: 546 KRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Chy12G214400 vs. NCBI nr
Match: XP_022970365.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 875 bits (2262), Expect = 0.0
Identity = 434/572 (75.87%), Postives = 501/572 (87.59%), Query Frame = 0


Query: 2   GSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAW FIKQVV GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNF G+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q  L +YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQS 241
           FAW PS+LIL++  SIRPI IRKHP ELKVFY LLYVSI+LAIFILFLT++QKQV+FSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS VV+LL +P+LIACREE LLYKL KQ     PSVT+S++ Q  P    P S  
Sbjct: 246 GYVGGASFVVSLLCVPILIACREELLLYKLNKQTAG--PSVTVSMVHQNPPTL--PASAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RG+DFTILQAVFS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLC 421
           ESLGYP +AIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL F+ AFL+TC+GQL 
Sbjct: 366 ESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGS+Y+A+I+IGFGFGAQNP+LFAVISEMFGL+ YS LFNCGQLAVPLGSYILNV
Sbjct: 426 IAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSYILNV 485

Query: 482 DIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYY 541
           D+VGKLYD EALREGK   G+G+ C+GAHCFGGSFTILAA TLFGA+VMLVLAYRTR++Y
Sbjct: 486 DVVGKLYDMEALREGKKIAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ +MEFYRLD+KK+I+D
Sbjct: 546 KRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

[Close]

BLAST of Chy12G214400 vs. NCBI nr
Match: XP_022964780.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 875 bits (2260), Expect = 1.34e-315
Identity = 435/572 (76.05%), Postives = 498/572 (87.06%), Query Frame = 0


Query: 2   GSEAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAK 61
           GSEAWAFIKQVV GRWFSV+AG++MMIGNGTTY+F TYSKV+K++FDYSQTQ+N LGFAK
Sbjct: 6   GSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINTLGFAK 65

Query: 62  DLGNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFG 121
           DLG+N GIIAGLL+E  PTWVLF+IGAFQNFTG+FLIWLS+T RI++P FWQMF+ VCFG
Sbjct: 66  DLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFILVCFG 125

Query: 122 SNSSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLL 181
           +NSSN++NTAIMVTS+RNFPDRRGIILGLLKGYVGIGGAI++Q+ L +YG  DPSN+VLL
Sbjct: 126 TNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPSNLVLL 185

Query: 182 FAWFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQS 241
           FAW PS+LIL++  SIRPI IRKHP+ELKVFY LLYVSI+LAIFILFLT++QKQV+FSQ+
Sbjct: 186 FAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQVVFSQA 245

Query: 242 AYASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTL 301
            Y  GAS VV LL +P+LIACREE LLYKL K+     PSV LS++ Q  P    P S  
Sbjct: 246 GYVGGASFVVCLLCVPILIACREELLLYKLNKET--AAPSVALSMLHQNPPTL--PTSAD 305

Query: 302 EEIAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIG 361
            EI EISPSC  NI NKP RG+DFTILQAVFS DM LICLATF+GCGSSLAAIDNLGQ+G
Sbjct: 306 SEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNLGQVG 365

Query: 362 ESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLC 421
           ESLGY  +AIGI VSWVSIFNFFGRV SGFISE +M KYKLPRPL FA AFL+TC+GQL 
Sbjct: 366 ESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCVGQLF 425

Query: 422 IAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNV 481
           IAYPFPGSLY+A+I+IGFGFGAQNP+LFAVISEMFGLK YS LFNCGQLA P GSYILNV
Sbjct: 426 IAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSYILNV 485

Query: 482 DIVGKLYDAEALREGKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYY 541
           D+VGKLYD EALRE K   G+G+ C+GAHCFGGSFT+LAA TLFGA+VM VLAYRTR++Y
Sbjct: 486 DVVGKLYDMEALRERKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVLAYRTREFY 545

Query: 542 RWDVYKNYKEDMWIPQAEMEFYRLDNKKNIDD 573
           + DVYKN+ E++WIPQ EMEFYRLDNKKNI+D
Sbjct: 546 KRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Chy12G214400 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 544.7 bits (1402), Expect = 9.1e-155
Identity = 280/577 (48.53%), Postives = 394/577 (68.28%), Query Frame = 0


Query: 4   EAWAFIKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDL 63
           E   F+     GRWF V+A  ++M   G TY+FGTYSK +KS   Y QT +N+LGF KDL
Sbjct: 5   ETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDL 64

Query: 64  GNNAGIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSN 123
           G N G+++GL++E  PTW +  IG+  NF GYF+IWL++T ++++P  WQM L +C G+N
Sbjct: 65  GANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGAN 124

Query: 124 SSNYSNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLLFA 183
           S N++NT  +VT ++NFP+ RG++LGLLKGYVG+ GAI TQ+   +YG  D  +++LL A
Sbjct: 125 SQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYG-HDSKSLILLIA 184

Query: 184 WFPSVLILLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQSAY 243
           W P+ + L+  + IR   + +   EL VFY  LY+SI LA+F++ + +++KQV FS++AY
Sbjct: 185 WLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAY 244

Query: 244 ASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTLEE 303
           A+ A++  ALLF+PL ++ ++E  ++ + K        V +    +++       + +  
Sbjct: 245 AASATICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAAKVNG 304

Query: 304 IAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIGES 363
             + + SC S + + P RG+D+TILQA+ S DM+++ +ATF G GSSL A+DNLGQIGES
Sbjct: 305 EEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGES 364

Query: 364 LGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLCIA 423
           LGYP   +  FVS VSI+N+FGRV SGF+SE ++ KYKLPRPLM  L  LL+C G L IA
Sbjct: 365 LGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIA 424

Query: 424 YPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNVDI 483
           +P PGS+Y+ASI++GF FGAQ PLLFA+ISE+FGLK+YS LFNCGQLA PLGSYILNV +
Sbjct: 425 FPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRV 484

Query: 484 VGKLYDAEALREGKNTTGRG--------VNCSGAHCFGGSFTILAAFTLFGALVMLVLAY 543
            G LYD EAL   K  T RG        + C G+ C+   F ILAA T FGALV L LA 
Sbjct: 485 TGMLYDKEAL---KQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAI 544

Query: 544 RTRDYYRWDVYKNYKEDMWIPQAEMEFYRLDNKKNID 573
           RTR++Y+ D+YK ++E    P++E E    D++K ++
Sbjct: 545 RTREFYKGDIYKKFRES---PESESELVP-DSRKAVE 573

[Close]

BLAST of Chy12G214400 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 520.4 bits (1339), Expect = 1.8e-147
Identity = 264/560 (47.14%), Postives = 375/560 (66.96%), Query Frame = 0


Query: 11  QVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAGII 70
           Q++ GRWF  +  +++M   G TY+FG YS  +K    Y QT +N+L F KDLG N G++
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 71  AGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYSNT 130
           AGLL+E  P W + +IGA  NF GYF+IWL++T RIS+P  W M L +C G+NS +++NT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 131 AIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLLFAWFPSVLI 190
             +VT ++NFP+ RG++LG+LKGYVG+ GAI+TQ+    YG ED   ++L+  W P+++ 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 191 LLISHSIRPILIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQSAYASGASVV 250
                +IR + +++   ELKVFY+ LY+S+ LA F++ + +  K   F+QS +   A+VV
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 251 VALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTLEEIAEI--- 310
           + LL LP+++   EE  L+K K+   N    + +     K+ +S       EE  E+   
Sbjct: 256 IVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEESKEVVEK 315

Query: 311 --SPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIGESLG 370
             +PSC + + N P RGDD+TILQA+FS+DM+++ LAT  G G +L AIDNLGQIG SLG
Sbjct: 316 VKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNSLG 375

Query: 371 YPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLCIAYP 430
           YP +++  FVS VSI+N++GRVVSG +SE+ +IKYK PRPLM  +  LL+C G L IA+ 
Sbjct: 376 YPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIAFN 435

Query: 431 FPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNVDIVG 490
            PG LYVAS++IGF FGAQ PLLFA+ISE+FGLK+YS L+N G +A P+GSY+LNV + G
Sbjct: 436 VPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRVAG 495

Query: 491 KLYDAEALREGK-----NTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDY 550
            LYD EA ++ K        G+ +NC G  CF  SF I+AA TLFG LV +VL  RT+ +
Sbjct: 496 YLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRTKKF 555

Query: 551 YRWDVYKNYKEDMWIPQAEM 561
           Y+ D+YK ++E     + EM
Sbjct: 556 YKSDIYKKFREKALAAEMEM 574

[Close]

BLAST of Chy12G214400 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 302.4 bits (773), Expect = 7.9e-82
Identity = 185/561 (32.98%), Postives = 304/561 (54.19%), Query Frame = 0


Query: 13  VEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAGIIAG 72
           +  RW    A M +    G  Y+FG+ S V+KS  +Y+Q +++ LG AKDLG++ G IAG
Sbjct: 13  INNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSRLGVAKDLGDSVGFIAG 72

Query: 73  LLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYSNTAI 132
            LSE +P W   ++GA QN  GY  +WL +T R      W M + +  G+N   Y NT  
Sbjct: 73  TLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCVLIFVGNNGETYFNTGA 132

Query: 133 MVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLLFAWFPSVLILL 192
           +V+ ++NFP  RG ++G+LKG+ G+GGAI++Q+   ++   +P++++L+ A  P+V+++ 
Sbjct: 133 LVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHS-SNPASLILMVAVTPAVVVVC 192

Query: 193 ISHSIRPILIRK--HPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQSAYASGASVV 252
           +   IRP+   K   P +   F  +  V ++LA +++ + + Q  V+ S +       V+
Sbjct: 193 LMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQDLVVVSHNVITVFTIVL 252

Query: 253 VALLFLPLLIACR--------------EEFLLYKLKKQNHNLD-PSVTLSIIDQKVPNS- 312
             +L +P+L+                 EE L+ K + Q   L  P + LS ++ + P   
Sbjct: 253 FVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQTPDLILSEVEDEKPKDV 312

Query: 313 --------HKPFSTLEE---IAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLAT 372
                   HK  + L+     A    +   N    PHRG+DFT+ QA+   D  LI  + 
Sbjct: 313 DLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTLTQALVKADFWLIFFSL 372

Query: 373 FAGCGSSLAAIDNLGQIGESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLP 432
             G GS L  IDNLGQ+ +SLGY      + VS +SI+NF GR+  G+ SEL++  Y  P
Sbjct: 373 LLGSGSGLTVIDNLGQMSQSLGY--DNTHVLVSMISIWNFLGRIGGGYFSELVVRDYAYP 432

Query: 433 RPLMFALAFLLTCIGQLCIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSI 492
           RP+  A+A L+  +G +  AY +PG++Y+ +++IG G+GA   ++ A  SE+FGLK +  
Sbjct: 433 RPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAIVPATASELFGLKKFGA 492

Query: 493 LFNCGQLAVPLGSYILNVDIVGKLYDAEALREGKNTT---GRGVNCSGAHCFGGSFTILA 542
           L+N   LA P GS + +  I   +YD EA R+   +       + C+G+ CF  +  I++
Sbjct: 493 LYNFLTLANPAGSLVFSGMIASSIYDREAERQAHGSVFDPDDALRCNGSICFFLTSLIMS 552

[Close]

BLAST of Chy12G214400 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 293.1 bits (749), Expect = 4.8e-79
Identity = 185/570 (32.46%), Postives = 304/570 (53.33%), Query Frame = 0


Query: 9   IKQVVEGRWFSVWAGMMMMIGNGTTYIFGTYSKVLKSEFDYSQTQVNMLGFAKDLGNNAG 68
           +K  +  RW    A M +    G  Y+FG+ S V+KS  +Y+Q Q++ LG AKDLG++ G
Sbjct: 9   VKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVG 68

Query: 69  IIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNYS 128
            +AG LSE +P W   ++G+ QN  GY  +WL +T R      W M + +  G+N   Y 
Sbjct: 69  FLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYF 128

Query: 129 NTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLLFAWFPSV 188
           NTA +V+ ++NFP  RG ++G+LKG+ G+GGAIL+QV   ++   D ++++ + A  PSV
Sbjct: 129 NTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHS-SDRASLIFMVAVAPSV 188

Query: 189 LILLISHSIRPILIRK--HPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQSAYASG 248
           +++ +   IRP+   +     +   F  +  V I+LA +++ + + +  +  S S   + 
Sbjct: 189 VVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAF 248

Query: 249 ASVVVALL----FLPLLIAC----------REEFLLYKLKKQNHNLD------PSVTLSI 308
             V+ A+L    F+P+  +C           EE LL   + Q+          P +  S 
Sbjct: 249 TVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSE 308

Query: 309 IDQKVPNSHKPFSTLE---EIAEISPSCLSNIC---------NKPHRGDDFTILQAVFSI 368
           ++ + P        +E    IA++    +               PHRG+DFT+ QA+   
Sbjct: 309 VEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKA 368

Query: 369 DMVLICLATFAGCGSSLAAIDNLGQIGESLGYPPQAIGIFVSWVSIFNFFGRVVSGFISE 428
           D  LI  +   G GS L  IDNLGQ+ +SLGY      +FVS +SI+NF GR+  G+ SE
Sbjct: 369 DFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGY--DNTHVFVSMISIWNFLGRIGGGYFSE 428

Query: 429 LMMIKYKLPRPLMFALAFLLTCIGQLCIAYPFPGSLYVASIVIGFGFGAQNPLLFAVISE 488
           L++  Y  PRP+  A+A L+  +G +  AY +PG++++ +++IG G+GA   ++ A  SE
Sbjct: 429 LIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASE 488

Query: 489 MFGLKHYSILFNCGQLAVPLGSYILNVDIVGKLYDAEALREGKNTTGRG---VNCSGAHC 542
           +FGLK +  L+N   LA P GS + +  I   +YD EA R+ + +       + C G+ C
Sbjct: 489 LFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQAQGSLFNPDDVLRCRGSIC 548

[Close]

BLAST of Chy12G214400 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 288.1 bits (736), Expect = 1.5e-77
Identity = 180/539 (33.40%), Postives = 300/539 (55.66%), Query Frame = 0


Query: 9   IKQVVEGRWFSVWAGMMMMIGNGTTYIF-GTYSKVLKSEFDYSQTQVNMLGFAKDLGNNA 68
           ++ +V  RW      M +    G  Y+F G+ S  +K+   Y+Q Q+ +LG AK+LG+  
Sbjct: 9   LELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAI 68

Query: 69  GIIAGLLSEFVPTWVLFMIGAFQNFTGYFLIWLSMTRRISQPAFWQMFLCVCFGSNSSNY 128
           G ++G LSE  P+WV+ ++GA QN  GY ++WL +T ++     W +F+ +  G+N   Y
Sbjct: 69  GFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETY 128

Query: 129 SNTAIMVTSLRNFPDRRGIILGLLKGYVGIGGAILTQVCLGLYGPEDPSNIVLLFAWFPS 188
            NTA +V+ + NFP+ RG ++G+LKG+ G+ GAILTQV L ++ P   S+++L+ A  P 
Sbjct: 129 YNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPP 188

Query: 189 VLILLISHSIRPI----LIRKHPEELKVFYHLLYVSIVLAIFILFLTMSQKQVIFSQSAY 248
           V++L +   +RP+          ++L+ F  +    +VLA+++L L + Q     +Q+  
Sbjct: 189 VVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTII 248

Query: 249 ASGASVVVALLFLPLLIACREEFLLYKLKKQNHNLDPSVTLSIIDQKVPNSHKPFSTLEE 308
            +  +++V  + +P+L+     F+         ++ P    S +DQ     H+   TL E
Sbjct: 249 TTSGAILVIFMVVPVLVPFSSVFI---SGNNVTSVKPEEGTSNVDQ-----HEA-RTLIE 308

Query: 309 IAEISPSCLSNICNKPHRGDDFTILQAVFSIDMVLICLATFAGCGSSLAAIDNLGQIGES 368
            ++  P         P  G+DFT+LQA+   D  LI ++   G GS +  IDNLGQI  S
Sbjct: 309 RSDRPPEKK----RAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYS 368

Query: 369 LGYPPQAIGIFVSWVSIFNFFGRVVSGFISELMMIKYKLPRPLMFALAFLLTCIGQLCIA 428
           LGY      IFVS +SI NF GRV  G+ SEL++ K  LPR L  ++   +  +G +  A
Sbjct: 369 LGY--SNTKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYA 428

Query: 429 YPFPGSLYVASIVIGFGFGAQNPLLFAVISEMFGLKHYSILFNCGQLAVPLGSYILNVDI 488
             +PG +YV +IVIG G+GA   +  A +S++FGLK +  L+N    A+P+GS++ +  I
Sbjct: 429 IDWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVI 488

Query: 489 VGKLYDAEALRE-GKNTTGRGVNCSGAHCFGGSFTILAAFTLFGALVMLVLAYRTRDYY 542
              +YD  A ++ G  T    + C+G+ C+  + ++++   L   ++ L + YRTR +Y
Sbjct: 489 ASNIYDYYARKQAGPTTETESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKFY 530

The following BLAST results are available for this feature:

BLAST of Chy12G214400 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	3.6e-31	26.85	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]

BLAST of Chy12G214400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CMS0	2.6e-290	87.61	uncharacterized protein LOC103502580 OS=Cucumis melo OX=3656 GN=LOC103502580 PE=...	[more]
A0A6J1HLS6	6.9e-251	76.22	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1I5D7	2.0e-250	75.87	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469...	[more]
A0A6J1HLT1	3.4e-250	76.05	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1I2B3	5.8e-250	75.52	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469...	[more]

BLAST of Chy12G214400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004147308.1	0.0	96.68	uncharacterized protein LOC101204150 [Cucumis sativus] >KAE8652748.1 hypothetica...	[more]
XP_008464773.1	0.0	87.61	PREDICTED: uncharacterized protein LOC103502580 [Cucumis melo]	[more]
XP_022964775.1	0.0	76.22	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata]	[more]
XP_022970365.1	0.0	75.87	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 prote...	[more]
XP_022964780.1	1.34e-315	76.05	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata]	[more]

BLAST of Chy12G214400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28120.1	9.1e-155	48.53	Major facilitator superfamily protein	[more]
AT2G39210.1	1.8e-147	47.14	Major facilitator superfamily protein	[more]
AT5G14120.1	7.9e-82	32.98	Major facilitator superfamily protein	[more]
AT3G01930.2	4.8e-79	32.46	Major facilitator superfamily protein	[more]
AT5G50630.1	1.5e-77	33.40	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (hystrix) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 340..539 e-value: 5.6E-11 score: 42.0
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 16..261 e-value: 7.3E-74 score: 248.4
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 311..547 e-value: 8.1E-12 score: 46.9
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 16..539
None	No IPR available	PANTHER	PTHR21576:SF29	PROTEIN NUCLEAR FUSION DEFECTIVE 4	coord: 3..552
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 3..552

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Chy12G214400.1	Chy12G214400.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity